BR112015011061A2 - EXPRESSION CASSETTE SUITABLE FOR EXPRESSION OF A POLYPEPTIDE OF INTEREST, EXPRESSION VECTOR, METHODS FOR PRODUCING A EUKARYOTIC HOST CELL AND POLYPEPTIDE OF INTEREST, AND USE OF A 5'UTR SEQUENCE IN COMBINATION WITH AN HCD33 SECRETIONAL LEADER SEQUENCE IN A CASSETTE OF EXPRESSION - Google Patents
EXPRESSION CASSETTE SUITABLE FOR EXPRESSION OF A POLYPEPTIDE OF INTEREST, EXPRESSION VECTOR, METHODS FOR PRODUCING A EUKARYOTIC HOST CELL AND POLYPEPTIDE OF INTEREST, AND USE OF A 5'UTR SEQUENCE IN COMBINATION WITH AN HCD33 SECRETIONAL LEADER SEQUENCE IN A CASSETTE OF EXPRESSIONInfo
- Publication number
- BR112015011061A2 BR112015011061A2 BR112015011061A BR112015011061A BR112015011061A2 BR 112015011061 A2 BR112015011061 A2 BR 112015011061A2 BR 112015011061 A BR112015011061 A BR 112015011061A BR 112015011061 A BR112015011061 A BR 112015011061A BR 112015011061 A2 BR112015011061 A2 BR 112015011061A2
- Authority
- BR
- Brazil
- Prior art keywords
- expression
- polypeptide
- interest
- cassette
- hcd33
- Prior art date
Links
- 229920001184 polypeptide Polymers 0.000 title abstract 7
- 102000004196 processed proteins & peptides Human genes 0.000 title abstract 7
- 108090000765 processed proteins & peptides Proteins 0.000 title abstract 7
- 108020003589 5' Untranslated Regions Proteins 0.000 title abstract 3
- 239000013604 expression vector Substances 0.000 title abstract 3
- 230000028327 secretion Effects 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/30—Vector systems having a special element relevant for transcription being an enhancer not forming part of the promoter region
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
Abstract
CASSETE DE EXPRESSÃO ADEQUADO PARA EXPRESSÃO DE UM POLIPEPTÍDEO DE INTERESSE, VETOR DE EXPRESSÃO, MÉTODOS PARA PRODUZIR UMA CÉLULA HOSPEDEIRA EUCARIÓTICA E UM POLIPEPTÍDEO DE INTERESSE, E USO DE UMA SEQUÊNCIA 5'UTR EM COMBINAÇÃO COM UMA SEQUÊNCIA LÍDER DE SECREÇÃO DE HCD33 EM UM CASSETE DE EXPRESSÃO. A presente invenção refere-se à descoberta de que a combinação de uma sequência de polinucleotídeos 5'UTR específica (vide SEQ ID NO: 1) e a sequência líder de secreção de hCD33 em um cassete de expressão para expressão de um polipeptídeo de interesse resulta em um nível de expressão surpreendentemente melhor do polipeptídeo de interesse comparado com os cassetes de expressão do estado da técnica. Com base nesta descoberta, a presente invenção proporciona, inter alia, novos cassetes de expressão, vetores de expressão e métodos para produção de um polipeptídeo de interesse com um rendimento elevado.EXPRESSION CASSETTE SUITABLE FOR EXPRESSION OF A POLYPEPTIDE OF INTEREST, EXPRESSION VECTOR, METHODS FOR PRODUCING A EUKARYOTIC HOST CELL AND POLYPEPTIDE OF INTEREST, AND USE OF A 5'UTR SEQUENCE IN COMBINATION WITH AN HCD33 SECRETIONAL LEADER SEQUENCE IN A CASSETTE OF EXPRESSION. The present invention relates to the discovery that the combination of a specific 5'UTR polynucleotide sequence (see SEQ ID NO: 1) and the hCD33 secretion leader sequence in an expression cassette for expression of a polypeptide of interest results in at a surprisingly better level of expression of the polypeptide of interest compared to prior art expression cassettes. Based on this discovery, the present invention provides, inter alia, new expression cassettes, expression vectors and methods for producing a polypeptide of interest in high yield.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261728459P | 2012-11-20 | 2012-11-20 | |
| US61/728,459 | 2012-11-20 | ||
| PCT/EP2013/074114 WO2014079819A1 (en) | 2012-11-20 | 2013-11-18 | Optimized expression cassette for expressing a polypeptide with high yield |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| BR112015011061A2 true BR112015011061A2 (en) | 2017-08-22 |
| BR112015011061A8 BR112015011061A8 (en) | 2020-03-17 |
| BR112015011061B1 BR112015011061B1 (en) | 2022-09-27 |
Family
ID=49585414
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112015011061-4A BR112015011061B1 (en) | 2012-11-20 | 2013-11-18 | EXPRESSION CASSETTE SUITABLE FOR EXPRESSION OF A POLYPEPTID OF INTEREST, EXPRESSION VECTOR, METHODS FOR PRODUCING A EUKARYOTIC HOST CELL AND A POLYPEPTIDE OF INTEREST, AND USE OF A 5'UTR SEQUENCE IN COMBINATION WITH A SECRETION LEAD SEQUENCE OF HCD33 IN A CASSETTE OF EXPRESSION |
Country Status (18)
| Country | Link |
|---|---|
| US (2) | US9663797B2 (en) |
| EP (1) | EP2922962B1 (en) |
| JP (1) | JP6388592B2 (en) |
| KR (1) | KR102286389B1 (en) |
| CN (2) | CN113981002A (en) |
| AU (1) | AU2013349844B2 (en) |
| BR (1) | BR112015011061B1 (en) |
| CA (1) | CA2891388C (en) |
| DK (1) | DK2922962T3 (en) |
| ES (1) | ES2620325T3 (en) |
| IL (1) | IL238703B (en) |
| LT (1) | LT2922962T (en) |
| MX (1) | MX359950B (en) |
| PL (1) | PL2922962T3 (en) |
| PT (1) | PT2922962T (en) |
| RU (1) | RU2682884C2 (en) |
| SG (1) | SG11201503326PA (en) |
| WO (1) | WO2014079819A1 (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114561386B (en) * | 2022-02-21 | 2024-05-28 | 湖南农业大学 | Gene element with intramolecular chaperone characteristic and application thereof |
| CN118667797A (en) * | 2023-03-14 | 2024-09-20 | 北京据德医药科技有限公司 | Recombinant acid alpha-glucosidase and application thereof |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3431140A1 (en) | 1984-08-24 | 1986-03-06 | Behringwerke Ag, 3550 Marburg | ENHANCER FOR EUKARYOTIC EXPRESSION SYSTEMS |
| GB8717430D0 (en) | 1987-07-23 | 1987-08-26 | Celltech Ltd | Recombinant dna product |
| WO1994004678A1 (en) | 1992-08-21 | 1994-03-03 | Casterman Cecile | Immunoglobulins devoid of light chains |
| DK1716233T3 (en) | 2004-01-30 | 2009-10-26 | Maxygen Holdings Ltd | Regulated stop codon readout |
| EP1788088B1 (en) * | 2004-07-27 | 2009-05-27 | Riken | Vector expressing human n-deacetylase/n-sulfotransferase 2 |
| CA2651478C (en) | 2006-05-17 | 2015-02-03 | Josef Endl | Polypeptide producing cells |
| WO2008023247A2 (en) * | 2006-08-23 | 2008-02-28 | Selexis S.A. | Matrix attachment regions (mars) for increasing transcription and uses thereof |
| US7790676B2 (en) * | 2007-03-28 | 2010-09-07 | Zymogenetics, Inc. | Soluble IL-17RA/RC fusion proteins |
| PT2227546T (en) * | 2007-12-21 | 2016-08-12 | Novartis Ag | Mammalian expression vector |
| WO2009080759A1 (en) | 2007-12-21 | 2009-07-02 | Novartis Ag | Organic compounds |
| WO2010006415A1 (en) * | 2008-06-30 | 2010-01-21 | Atgcell Inc. | Mammalian cell expression vectors and utilization |
| JP5788796B2 (en) | 2008-08-28 | 2015-10-07 | ノバルティス アーゲー | Cell surface presentation of polypeptide isoforms by stop codon readthrough |
| DK2401377T3 (en) | 2009-02-27 | 2016-08-29 | Novartis Ag | Expression vector system comprising two selection markers |
-
2013
- 2013-11-18 EP EP13792030.2A patent/EP2922962B1/en active Active
- 2013-11-18 PT PT137920302T patent/PT2922962T/en unknown
- 2013-11-18 ES ES13792030.2T patent/ES2620325T3/en active Active
- 2013-11-18 MX MX2015006376A patent/MX359950B/en active IP Right Grant
- 2013-11-18 WO PCT/EP2013/074114 patent/WO2014079819A1/en active Application Filing
- 2013-11-18 KR KR1020157016125A patent/KR102286389B1/en active IP Right Grant
- 2013-11-18 SG SG11201503326PA patent/SG11201503326PA/en unknown
- 2013-11-18 US US14/443,863 patent/US9663797B2/en active Active
- 2013-11-18 CA CA2891388A patent/CA2891388C/en active Active
- 2013-11-18 CN CN202111282575.3A patent/CN113981002A/en active Pending
- 2013-11-18 BR BR112015011061-4A patent/BR112015011061B1/en active IP Right Grant
- 2013-11-18 LT LTEP13792030.2T patent/LT2922962T/en unknown
- 2013-11-18 CN CN201380060589.8A patent/CN104968791B/en active Active
- 2013-11-18 PL PL13792030T patent/PL2922962T3/en unknown
- 2013-11-18 JP JP2015543403A patent/JP6388592B2/en active Active
- 2013-11-18 DK DK13792030.2T patent/DK2922962T3/en active
- 2013-11-18 AU AU2013349844A patent/AU2013349844B2/en active Active
- 2013-11-18 RU RU2015124041A patent/RU2682884C2/en active
-
2015
- 2015-05-07 IL IL238703A patent/IL238703B/en active IP Right Grant
-
2017
- 2017-04-21 US US15/493,837 patent/US11230718B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| IL238703B (en) | 2019-02-28 |
| DK2922962T3 (en) | 2017-03-27 |
| AU2013349844B2 (en) | 2017-03-02 |
| IL238703A0 (en) | 2015-06-30 |
| WO2014079819A1 (en) | 2014-05-30 |
| US20170356006A1 (en) | 2017-12-14 |
| RU2682884C2 (en) | 2019-03-22 |
| LT2922962T (en) | 2017-04-10 |
| KR20150085537A (en) | 2015-07-23 |
| CN104968791B (en) | 2021-11-19 |
| PL2922962T3 (en) | 2017-06-30 |
| CA2891388C (en) | 2021-09-14 |
| EP2922962B1 (en) | 2016-12-21 |
| MX2015006376A (en) | 2015-10-05 |
| ES2620325T3 (en) | 2017-06-28 |
| CN104968791A (en) | 2015-10-07 |
| CA2891388A1 (en) | 2014-05-30 |
| KR102286389B1 (en) | 2021-08-05 |
| JP2015535427A (en) | 2015-12-14 |
| MX359950B (en) | 2018-10-17 |
| US11230718B2 (en) | 2022-01-25 |
| US9663797B2 (en) | 2017-05-30 |
| US20160186206A1 (en) | 2016-06-30 |
| CN113981002A (en) | 2022-01-28 |
| BR112015011061A8 (en) | 2020-03-17 |
| SG11201503326PA (en) | 2015-06-29 |
| PT2922962T (en) | 2017-03-29 |
| EP2922962A1 (en) | 2015-09-30 |
| AU2013349844A1 (en) | 2015-05-21 |
| JP6388592B2 (en) | 2018-09-12 |
| BR112015011061B1 (en) | 2022-09-27 |
| RU2015124041A (en) | 2017-01-10 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B06F | Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette] | ||
| B06F | Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette] | ||
| B06I | Publication of requirement cancelled [chapter 6.9 patent gazette] |
Free format text: ANULADA A PUBLICACAO CODIGO 6.6.1 NA RPI NO 2462 DE 13/03/2018 POR TER SIDO INDEVIDA. |
|
| B06U | Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette] | ||
| B06A | Patent application procedure suspended [chapter 6.1 patent gazette] | ||
| B09A | Decision: intention to grant [chapter 9.1 patent gazette] | ||
| B16A | Patent or certificate of addition of invention granted [chapter 16.1 patent gazette] |
Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 18/11/2013, OBSERVADAS AS CONDICOES LEGAIS |