BR112014012208A2 - fibrinogen assay - Google Patents

fibrinogen assay

Info

Publication number
BR112014012208A2
BR112014012208A2 BR112014012208A BR112014012208A BR112014012208A2 BR 112014012208 A2 BR112014012208 A2 BR 112014012208A2 BR 112014012208 A BR112014012208 A BR 112014012208A BR 112014012208 A BR112014012208 A BR 112014012208A BR 112014012208 A2 BR112014012208 A2 BR 112014012208A2
Authority
BR
Brazil
Prior art keywords
fibrinogen
sample
amount
monoclonal antibody
intact
Prior art date
Application number
BR112014012208A
Other languages
Portuguese (pt)
Inventor
Deanglis Ashley
Burcoglu Elif
Original Assignee
Ethicon Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ethicon Inc filed Critical Ethicon Inc
Publication of BR112014012208A2 publication Critical patent/BR112014012208A2/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/86—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood coagulating time or factors, or their receptors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Electrochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

resumo patente de invenção: "ensaio de fibrinogênio". a presente invenção refere-se a um método de detecção de fibrinogênio intacto, que compreende as etapas de: a) fornecer uma amostra contendo ao menos certa quantidade de fibrinogênio opcionalmente convertida, ao menos em parte, em fibrina, e contendo opcionalmente trombina; b) solubilizar a amostra em uma solução solubilizante que inibe a atividade da trombina; c) após sds-page opcional, transferir/aplicar uma porção da dita amostra em uma membrana de ligação à proteína; d) reagir o fibrinogênio com um anticorpo monoclonal primário capaz de se ligar à porção do fibrinopeptídeo a; e e) detectar a quantidade de fibrinogênio intacto na amostra pela quantificação da quantidade do anticorpo monoclonal primário ligado.patent summary: "fibrinogen assay". The present invention relates to an intact fibrinogen detection method comprising the steps of: a) providing a sample containing at least a certain amount of fibrinogen optionally converted at least in part to fibrin and optionally containing thrombin; b) solubilize the sample in a solubilizing solution that inhibits thrombin activity; c) after optional sds-pages transfer / apply a portion of said sample to a protein binding membrane; d) reacting the fibrinogen with a primary monoclonal antibody capable of binding to the fibrinopeptide portion a; and e) detecting the amount of intact fibrinogen in the sample by quantifying the amount of bound primary monoclonal antibody.

BR112014012208A 2011-11-21 2012-11-19 fibrinogen assay BR112014012208A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US13/300,795 US8900822B2 (en) 2011-11-21 2011-11-21 Fibrinogen assay
PCT/US2012/065770 WO2013078107A1 (en) 2011-11-21 2012-11-19 Fibrinogen assay

Publications (1)

Publication Number Publication Date
BR112014012208A2 true BR112014012208A2 (en) 2017-05-30

Family

ID=48427300

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112014012208A BR112014012208A2 (en) 2011-11-21 2012-11-19 fibrinogen assay

Country Status (10)

Country Link
US (2) US8900822B2 (en)
EP (1) EP2791351B1 (en)
JP (1) JP6181062B2 (en)
CN (1) CN103946391A (en)
AU (1) AU2012340850B2 (en)
BR (1) BR112014012208A2 (en)
CA (1) CA2855139C (en)
ES (1) ES2644283T3 (en)
RU (1) RU2619256C2 (en)
WO (1) WO2013078107A1 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8900822B2 (en) * 2011-11-21 2014-12-02 Ethicon, Inc. Fibrinogen assay
CN104458367B (en) * 2014-11-06 2019-03-26 上海长岛生物技术有限公司 A kind of d-dimer and the compound quality-control product of FDP and preparation method thereof
EP3234613B1 (en) * 2014-12-18 2019-02-20 Koninklijke Philips N.V. Device, system and method for determining a fibrinogen level in a blood sample
KR101700513B1 (en) * 2015-06-05 2017-01-31 인천대학교 산학협력단 Method for detecting thrombin using surface-modified gold electrode
CN105548562A (en) * 2015-12-22 2016-05-04 山东博科生物产业有限公司 An immunoturbidimetric detection kit for fibrinogen
US10268369B2 (en) * 2017-06-06 2019-04-23 Polycom, Inc. Detecting erasure gestures in an electronic presentation system
RU2712643C1 (en) * 2019-10-23 2020-01-30 федеральное государственное бюджетное учреждение "Национальный медицинский исследовательский центр профилактической медицины" Министерства здравоохранения Российской Федерации (ФГБУ "НМИЦ ПМ" Минздрава России) Method for determining fibrinogen and evaluating its functionality

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61178927A (en) * 1981-06-25 1986-08-11 セラフアルム　ジ−エムビ−エイチ　アンド　カンパニ−　ケイジイ Dry medicine containing fibrinogen and manufacture
US4438209A (en) 1981-07-17 1984-03-20 Mallinckrodt, Inc. Radioimmunoassay for fibrinopeptide A
JPH0284175A (en) 1988-06-10 1990-03-26 New York Blood Center Inc Monoclonal antibody specific to human fibrinopeptide a
SE9001582D0 (en) * 1990-05-03 1990-05-03 Dagny Birgitta Hessel SET FOR QUANTITATIVE DETERMINATION OF FIBRINOGEN, FIBRONECTIN, ALFA-2 ANTIPLASMIN OR FACTOR XIII
AU644205B2 (en) 1990-08-23 1993-12-02 New York Blood Center, Inc., The Assays using a soluble fibrin-like monomer
ES2300102T3 (en) 1993-11-02 2008-06-01 Mitsubishi Kagaku Iatron, Inc. HUMAN SOLUBLE ANTI-FIBRINE ANTIBODIES, HYBRIDOMA THAT PRODUCES THEM AND IMMUNO TEST PROCEDURE.
US5817768A (en) 1995-06-07 1998-10-06 The New York Blood Center, Inc. Monospecific antibodies against a subunit of fibrinogen
US5876947A (en) 1997-07-25 1999-03-02 The New York Blood Center, Inc. Monospecific antibody reactive with Fibrinogen and fibrinopeptide B
EP1281089A1 (en) 2000-02-23 2003-02-05 Besst-Test APS METHOD FOR CORRELATING BLOOD COAGULATION ACTIVITY WITH MARKERS IN BODY FLUIDS, e.g. URINE
US6448024B1 (en) * 2000-10-03 2002-09-10 Roche Diagnostics Corporation Method, reagent, cartridge, and device for determining fibrinogen
FR2831170B1 (en) 2001-10-19 2004-03-19 Inst Nat Sante Rech Med MODIFIED PROTEINS C DIRECTLY ACTIVABLE WITH THROMBIN
DE10261126A1 (en) * 2002-08-13 2004-03-04 Aventis Behring Gmbh Storage-stable, liquid fibrinogen formulation
US9358318B2 (en) 2004-10-20 2016-06-07 Ethicon, Inc. Method of making a reinforced absorbable multilayered hemostatic wound dressing
JP5058808B2 (en) 2004-10-20 2012-10-24 エシコン・インコーポレイテッド Reinforced absorbent multilayer fabric for medical devices and method for producing the same
US20060093655A1 (en) 2004-10-20 2006-05-04 Lillian Bar Method for making a reinforced absorbable multilayered hemostatic wound dressing
WO2007030531A2 (en) 2005-09-06 2007-03-15 Molecular Image Inc. Reagents for testing and molecular imaging of liver cancer
US7790410B2 (en) 2008-04-25 2010-09-07 General Electric Company Method and apparatus for determining hemocompatibility
RU2462721C2 (en) * 2008-05-22 2012-09-27 Этикон, Инк. Method for thrombin and fibrinogen activity and functionality test
JP5539332B2 (en) * 2008-05-22 2014-07-02 エシコン・インコーポレイテッド Protein assay
US8900822B2 (en) * 2011-11-21 2014-12-02 Ethicon, Inc. Fibrinogen assay

Also Published As

Publication number Publication date
JP2014533835A (en) 2014-12-15
US20130130282A1 (en) 2013-05-23
CN103946391A (en) 2014-07-23
AU2012340850A1 (en) 2014-07-03
RU2619256C2 (en) 2017-05-12
WO2013078107A1 (en) 2013-05-30
CA2855139A1 (en) 2013-05-30
US20150050673A1 (en) 2015-02-19
US8900822B2 (en) 2014-12-02
JP6181062B2 (en) 2017-08-16
EP2791351A4 (en) 2015-01-07
US9017955B2 (en) 2015-04-28
RU2014125266A (en) 2015-12-27
EP2791351B1 (en) 2017-08-09
CA2855139C (en) 2020-01-14
EP2791351A1 (en) 2014-10-22
ES2644283T3 (en) 2017-11-28
AU2012340850B2 (en) 2017-08-24

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Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according art. 34 industrial property law
B06U Preliminary requirement: requests with searches performed by other patent offices: suspension of the patent application procedure
B11B Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements