BR102019000701A2 - INNOVATIVE AGROCHEMICAL COMBINATIONS - Google Patents

Abstract

the present invention discloses a combination for treating seeds with fipronil; azoxystrobin; at least one benzimidazole fungicide; and a multisite fungicide or a neonicotinoid insecticide.

Description

The present invention relates to agrochemical combinations for the control of plant pests.

More particularly, the present invention relates to innovative combinations, to compositions comprising such combinations, and to methods of using the combinations for the treatment of plant propagating material.

BACKGROUND AND PREVIOUS TECHNIQUE:

[0002] The combination of insecticides and fungicides is used to broaden the spectrum of fungi and insect pest control, reduce the dosage, thus reducing the environmental impact, and decreasing the chances of resistance development. The combination of insecticides and fungicides sometimes demonstrates an additive or synergistic effect, which results in improved pest control.

[0003] Damage to seeds caused by fungi and insect pests is another important concern for the farmer. There are several diseases like seed rot etc. which reduce germination rates and cause a significant decrease in yield. The treatment of the seed with combinations of insecticides and fungicides helps to reduce the damage caused by such soil pests. Another advantage of treating seeds or other plant propagating material is improving germination rates, increasing yield and improving plant health.

[0004] There are many combinations of insecticides and fungicides known in the art for the control of soil-borne pests. For example, US5877194A teaches combinations of fipronil and triticonazole for seed treatment. US7732374B2 teaches combinations of protioconazole and insecticides to control pests in seeds, soils, etc.

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2/28 [0005] However, there is a need to improve these combinations. Combinations of isolated assets used over a long period of time resulted in resistance. With the beginning of resistance to certain pests, there is a need in the art for a combination of assets that reduces the chances of resistance and improves the spectrum of disease and pest control.

OBJECTIVES OF THE INVENTION:

[0006] Therefore, an objective of the present invention is to provide improved combinations of insecticides and fungicides for the control of soil-borne pests.

[0007] Another objective of the present invention is to provide a method and composition for controlling insect pests and fungal diseases around plant propagating material.

[0008] Yet another objective of the present invention is to provide improved combinations of insecticides and fungicides that promote plant health.

[0009] Modalities of the present invention can therefore alleviate one or more of the problems mentioned above.

SUMMARY OF THE INVENTION:

[0010] Thus, an aspect of the present invention can provide combinations of fipronil; azoxystrobin; at least one benzimidazole fungicide; and a multisite fungicide or a neonicotinoid insecticide.

[0011] Another aspect of the present invention can provide a method to improve yield in agricultural crops, the method comprising applying, to a plant propagating material of said culture, a combination comprising fipronil, azoxystrobin, at least one benzimidazole fungicide and a multisite fungicide or a neonicotinoid insecticide.

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3/28 [0012] Yet another aspect of the present invention can provide a method for improving plant health, the method comprising applying to a plant propagating material of said culture, a combination comprising fipronil, azoxystrobin, a benzimidazole fungicide and a multisite fungicide or a neonicotinoid insecticide.

[0013] Another aspect of the present invention can provide a composition for the treatment of plant propagating material, the composition comprising fipronil, azoxystrobin, at least one benzimidazole fungicide and a multisite fungicide or a neonicotinoid insecticide.

[0014] In another aspect, the present invention can provide a method for controlling pests by treating a plant propagating material with a composition comprising fipronil, azoxystrobin, at least one benzimidazole fungicide and a multisite fungicide or a neonicotinoid insecticide.

[0015] In yet another aspect, the present invention can provide a composition to improve the health of the plant, the method comprising applying to a plant propagating material of said culture a combination comprising fipronil, azoxystrobin, a benzimidazole fungicide and a multisite fungicide or a neonicotinoid insecticide.

DETAILED DESCRIPTION OF THE INVENTION [0016] The term plant propagation material refers to parts of the plant, such as seeds, that can be used for the propagation of the plant and vegetative plant material, such as seedlings and tubers (for example, potatoes). For example, seeds (in the strict sense), roots, fruits, tubers, bulbs, rhizomes, parts of plants can be mentioned. Germinated plants or young plants, which can be

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4/28 transplanted after germination or after emergence from the soil. The term insecticide refers to the ability of a substance to increase mortality or to inhibit the rate of growth of insects. The term fungicide refers to the ability of a substance to decrease or inhibit the growth of fungi. The term amount of agronomically acceptable active ingredient refers to an amount of an active ingredient that kills or inhibits the disease of the plant that you want to control, in an amount not significantly toxic to the plant to be treated. The term insect control or control means to inhibit, through a toxic effect, the ability of pest insects to survive, grow, feed and / or reproduce, or limit damage or losses related to insects in crop plants, or denotes control and disease prevention. The effects of control include all deviations from natural development, for example: death, delay or decrease in fungal disease or insect pest. Controlling insects may or may not mean killing insects, although it preferably means killing insects. The term amount of agronomically acceptable active ingredient refers to an amount of an active ingredient that kills or inhibits the disease of the plant that you want to control, in an amount not significantly toxic to the plant to be treated.

[0017] The term enhanced seed vigor refers to the properties of the seed that determine the potential for rapid and uniform emergence, and the development of seedlings under a wide range of field conditions, compared to the same characteristic in a plant control that was grown under the same conditions in the absence of the method of the invention. These traits can include early and / or improved germination, improved emergence, improved root growth, a developed root system, nodulation

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5/28 increased root, increased sprout growth, improved plant height, increased plant weight (fresh or dried), larger leaf blades, greener leaf color, higher pigment content, greater photosynthetic activity, flowering precocious, longer panicles, precocious grain maturity, greater seed size, fruit or pod, greater number of pods or ears, greater number of seeds per pod or ear, greater seed mass, improved seed filling, senescence delay, and improved plant vitality.

[0018] The present inventors observed unexpected synergy when fipronil and azoxystrobin were combined with a benzimidazole fungicide and either a multisite fungicide or a neonicotinoid insecticide. More specifically, the present inventors discovered unexpected synergy when fipronil and azoxystrobin were combined with carbendazim or methyl and imidacloprid thiophanate or a multisite fungicide, or both.

[0019] Fipronil is a phenylpyrazole insecticide that acts on the central nervous system of insects, blocking chloride channels controlled by GABA and chloride channels controlled by glutamate. Its chemical name is (RS) -5amino-1- [2,6-dichloro-4- (trifluoromethyl) -phenyl] -4 (trifluoromethylsulfinyl) -1H-pyrazol-3-carbonitrile.

[0020] Azoxystrobin is a systemic fungicide belonging to the class of strobilurin compounds. Its mode of action is due to the fixation to the Qo site of complex III of the mitochondrial electron transport chain, thus preventing the production of ATP. Its chemical name is methyl (E) -2- {2- [6- (2-cyanophenoxy) pyrimidin-4yloxy] phenyl} -3-methoxyacrylate.

[0021] Benzimidazole fungicides inhibit mitosis and cell division in fungi. They are broad spectrum fungicides that are known to control a wide variety of fungi.

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6/28 [0022] Neonicotinoid insecticides are systemic with contact and stomach action. Acetylcholine receptor (nAChR) agonist. These insecticides are known to control a wide spectrum of insect pests.

[0023] Multisite fungicides are known to attack fungi at multiple sites. These fungicides are used to control broad spectrum disease. Known multisite fungicides include, but are not limited to, dithiocarbamates, phthalimides, chloronitriles, inorganic fungicides, sulfamides, bis-guanidines, triazines, quinones, quinoxaline, dicocarboxamides, etc.

[0024] The present inventors believe that the combination of the present invention surprisingly results in a synergistic action. The combinations of the present invention allow for a broad spectrum of pest control and surprisingly improved the vigor and productivity of the plants. The broad spectrum of the present combination also provides a solution to prevent the development of resistance.

[0025] Thus, an aspect of the present invention can provide combinations comprising:

i) fipronil;

ii) azoxystrobin;

iii) a benzimidazole fungicide; and iv) at least one active agent selected from a neonicotinoid insecticide or a multisite fungicide, or both.

[0026] In one embodiment, the benzimidazole fungicide is selected from at least one of albendazole, benomile, carbendazim, chlorphenazole, cipendazole, debacarb, fuberidazole, mecarbinzide, rabenzazole, thiabendazole, furoate, thiophanate, methyl thiophanate.

[0027] In one embodiment, the benzimidazole fungicide is selected from benomile, carbendazim and thiophanate or methyl thiophanate.

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[0028] In one mode, The fungicide in benzimidazole is carbendazim. [0029] In one mode, O fungicide in benzimidazole is methyl thiophanate. [0030] In one mode, O fungicide multisite is one fungicide selected from group that It consists in

dithiocarbamates, phthalimides, chloronitriles, inorganic fungicides, sulfamides, bis-guanidines, triazines, quinones, quinoxalines and dicarboxamides.

[0031] In one embodiment, the dithiocarbamate fungicide selected from asamobam, asomato, azithirame, carbamorph, cufranebe, cuprobame, disulfirame, ferbame, metam, nabame, tecorame, tirame, urbacida, zirame, dazomete, eteme, milnebe, mannobre, mannebe, maneuvers , maneb, methram, polycarbamate, propineb and zineb.

[0032] In a preferred modality, the fungicide of

dithiocarbamate is selected among manebe, mancozebe and zineb. [0033] In an modality, the dithiocarbamate fungicide is mancozebe. [0034] In an modality, the multisite fungicide is one

phthalimide fungicide selected from captana, captafol and folpet.

[0035] In one modality, the multisite fungicide it is a fungicide in chloronitrile, as chlorothalonil. [0036] In one modality, the multisite fungicide it is a

sulfamide fungicide selected from diclofluanide and tolylfluanide.

[0037] In one embodiment, the multisite fungicide is a bis-guanidine fungicide selected from guazatin and iminoctadine.

[0038] In one embodiment, the multisite fungicide is a triazine fungicide selected from anilazine.

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8/28 [0039] In one embodiment, the multisite fungicide is a quinone dithianone fungicide.

[0040] In one embodiment, the multisite fungicide is a quinoxaline fungicide selected from quinomethionate and chloroquine.

[0041] In one embodiment, the multisite fungicide is a dicarboxamide fungicide selected from fluoroimide.

[0042] In one embodiment, the multisite fungicide is an inorganic fungicide selected from copper fungicides, including copper (II) hydroxide, copper oxychloride, copper (II) sulfate, basic copper sulfate, Bordeaux mixture, copper salicylate,

C7H4O3 * Cu, cuprous oxide

Cu2O; or sulfur.

0043]

In one embodiment, neonicotinoid insecticide can be selected from the group consisting of acetamipride, clothianidin, imidacloprid, nitenpyram, nitiazine, thiaclopride and thiamethoxam.

[0044] In a preferred embodiment, the neonicotinoid insecticide is selected from the group consisting of acetamipride, thiamethoxam and imidaclopride.

[0045] Thus, one embodiment of the present invention can provide combinations for the treatment of plant propagating material, which comprise:

i) fipronil;

ii) azoxystrobin;

iii) a benzimidazole fungicide, selected from carbendazim or methyl thiophanate; and iv) at least one active agent selected from imidacloprid, acetamiprid, thiamethoxam, mancozeb, maneb, zineb, folpete, chlorothalonil and tribasic copper sulfate.

[0046] Another embodiment of the present invention can provide combinations for the treatment of plant propagating material, which comprise:

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i) fipronil;

ii) azoxystrobin;

iii) a benzimidazole fungicide selected from carbendazim or methyl thiophanate; and iv) imidaclopride.

[0047] Another embodiment of the present invention can provide combinations comprising:

i) fipronil;

ii) azoxystrobin;

iii) a benzimidazole fungicide selected from carbendazim or methyl thiophanate; and iv) mancozebe.

[0048] Another modality of gift invention can to provide combinations comprising: i) fipronil; ii) azoxystrobin; iii) carbendazim; and iv) imidaclopride. [0049] Another modality of combinations comprising: i) fipronil; ii) azoxystrobin; iii) carbendazim; and iv) mancozebe. gift invention can to provide [0050] Another modality of combinations comprising: i) fipronil; ii) azoxystrobin; iii) methyl thiophanate; and iv) mancozebe. gift invention can to provide [0051] Another modality of combinations comprising: gift invention can to provide

i) fipronil;

ii) azoxystrobin;

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Iii) methyl thiophanate; and iv) imidaclopride.

[0052] Another embodiment of the present invention can provide combinations for the treatment of plant propagating material, which comprise:

i) fipronil;

ii) azoxystrobin;

iii) a benzimidazole fungicide, selected from carbendazim or methyl thiophanate; and iv) acetamiprid.

[0053] Another embodiment of the present invention can provide combinations for the treatment of plant propagating material, which comprise:

i) fipronil;

ii) azoxystrobin;

iii) a benzimidazole fungicide, selected from carbendazim or methyl thiophanate; and iv) thiamethoxam.

[0054] Yet another embodiment of the present invention can provide combinations for the treatment of plant propagating material, which comprise:

i) fipronil;

ii) azoxystrobin;

iii) a benzimidazole fungicide, selected from carbendazim or methyl thiophanate; and iv) chlorothalonil.

[0055] Yet another embodiment of the present invention can provide combinations for the treatment of plant propagating material, which comprise:

i) fipronil;

ii) azoxystrobin;

iii) a benzimidazole fungicide, selected from carbendazim or methyl thiophanate; and iv) tribasic copper sulfate.

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11/28 [0056] In one embodiment, the fungicides constituting the combination of the present invention can be mixed in a ratio of (1 to 80) :( 1 to 80): (1 to 80) :( 1 to 80) of fipronil , azoxystrobin, benzimidazole fungicide, neonicotinoid or multisite fungicide, respectively.

[0057] The combinations of the present invention can be mixed with suitable agrochemical adjuvants to form compositions.

[0058] Thus, an aspect of the present invention can provide a seed treatment composition comprising:

i) fipronil;

ii) azoxystrobin;

iii) a benzimidazole fungicide selected from carbendazim or methyl thiophanate;

iv) an active ingredient selected from a neonicotinoid or a multisite fungicide, or both; and

v) agrochemically acceptable adjuvants.

[0059] Another aspect of the present invention can provide a seed treatment composition comprising:

i) fipronil;

ii) azoxystrobin;

iii) a benzimidazole fungicide selected from carbendazim or methyl thiophanate;

iv) at least one active agent selected from imidacloprid, acetamipride, thiamethoxam, mancozeb, maneb, zineb, folpete, chlorothalonil and tribasic copper sulfate; and

v) agrochemically acceptable adjuvants.

[0060] In one embodiment, the total amount of fipronil in the composition can typically be in the range of 0.1 to 99% by weight, preferably 0.2 to 90% by weight. The total amount of azoxystrobin in the composition may be

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12/28 in the range of 0.1 to 99% by weight. The total amount of benzimidazole inhibitor in the composition can be in the range of 0.1 to 99%, by weight. The total amount of neonicotinoids in the composition can be in the range of 0.1 to 99% by weight, or the total amount of multisite fungicide in the composition can be in the range of 0.1 to 99% by weight.

[0061] In one embodiment, the fungicides and insecticides that make up the composition of the present invention can be mixed by adding in a ratio of (1 to 80) :( 1 to 80) :( 1 to 80) :( 1 to 80) of fipronil, azoxystrobin, benzimidazole fungicide, and neonicotinoid or multisite fungicide, respectively.

[0062] In one embodiment, the constituents of the composition of the present invention can be applied, according to known application methods, to seeds and other plant propagating material or transplanted seedlings.

[00 63] In one embodiment of the present invention, the compositions can be formulated in solid or liquid formulations suitable for application to plant propagating material. The formulation can be produced as known formulation types, such as wettable powders (WP), solutions

LS), emulsions (ES), suspension concentrates (FS), water dispersible granules (WG), or aqueous suspension concentrates (CS).

[0064] formulation can be applied by means of various methods, such as methods using conventional treatment techniques and machines, such as fluidized bed techniques, the roller mill method, rotostatic seed treaters and drum coaters, spouted beds, etc. .

Pre- and post-coating procedures, such as separation by size, etc., can also be performed.

Such procedures are known in the art.

It is readily understood that plant propagating material will only be

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13/28 treated when it has already been removed from the plant and is ready to be reseeded.

[0065] In one embodiment, the treatment can take place before the plant propagation material is sown so that the seed material has been pre-treated with the combination. In particular, seed coating or seed pelleting is preferred in the treatment of the combinations according to the invention. As a result of the treatment, the active ingredients in the combination are adhered to the seed and, therefore, available for pest and / or disease control.

[0066] Formulations may contain standard agriculturally acceptable adjuvants, vehicles, diluents, emulsifiers, fillers, antifoam agents, thickening agents, antifreeze agents, biocides, pigments etc.

[0067] Suitable agricultural adjuvants and vehicles may include, but are not limited to, agricultural oil concentrates; methylated seed oils, emulsified methylated seed oil, nonylphenol ethoxylate; benzylcocoalkylimethyl quaternary ammonium salt; mixture of petroleum hydrocarbons, alkyl esters, organic acid and anionic surfactant;

C ₉ -C 11 alkyl polyglycoside;

phosphate alcohol ethoxylate;

ethoxylate

C12-C16) of natural primary alcohol;

block copolymer of di-sec-butylphenol EO-PO;

polysiloxane-methyl block;

nonylphenol ethoxylate, ammonium nitrate and urea;

synthetic tridecyl alcohol ethoxylate)

8EO);

tallow amine ethoxylate; dioleate-99 of PEG (400), alkyl sulfates, such as diethanolammonium lauryl sulfate; alkylarylsulfonate salts, such as calcium dodecylbenzenesulfonate; alkylenealkyl oxide addition products, such as C18 nonylphenol ethoxylate; alkylene-alcohol oxide addition products, such as C16-tridecyl alcohol ethoxylate; soaps, such as sodium stearate;

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14/28 alkyl naphthalene sulfonate salts, such as dibutyl naphthalenesulfonate; dialkyl esters of sulfosuccinate salts, such as sodium di (2-ethylhexyl) sulfosuccinate; sorbitol esters, such as sorbitol oleate; quaternary amines, such as lauryltrimethylammonium chloride; polyethylene glycol esters of fatty acids, such as polyethylene glycol stearate; block copolymers of ethylene oxide and propylene oxide; salts of mono and dialkyl phosphate esters; vegetable or seed oils, such as soybean oil, rapeseed / canola oil, olive oil, castor oil, sunflower seed oil, coconut oil, corn oil, cottonseed oil, flaxseed oil, oil palm oil, peanut oil, safflower oil, sesame oil, tung oil and the like; and esters of the above vegetable oils and, in certain embodiments, methyl esters.

[0068] Suitable liquid vehicles that can be employed in a composition of the present invention can include water or organic solvents. Organic solvents include, but are not limited to, petroleum or hydrocarbon fractions, such as mineral oil, aromatic solvents, paraffinic oils and the like; vegetable oils, such as soybean oil, seed oil

rapeseed, oil in olive, oil in castor, oil seed in sunflower, oil in coconut, oil in corn, oil seed in cotton, oil in palm, oil in peanut oil safflower, oil Sesame, oil of tung and the like; esters From

vegetable oils mentioned above; esters of dihydric, trihydric monoalcohols or polyalcohols or other lower polyalcohols (containing 4-6 hydroxy), such as 2-ethyl hexyl stearate, n-butyl oleate, isopropyl myristate, propylene glycol dioleate, di-succinate octyl, di-butyl adipate, di-octyl phthalate and the like;

esters of mono-, di- and polycarboxylic acids and the like. Organic solvents include, but are not limited to, toluene, xylene, petroleum naphtha, vegetable oil, acetone, methyl ketone and

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15/28 ethyl, cyclohexanone, trichlorethylene, perchlorethylene, ethyl acetate, amyl acetate, butyl acetate, propylene glycol monomethyl ether and diethylene glycol monomethyl ether, methyl alcohol, ethyl alcohol, isopropyl alcohol, amyl alcohol, ethylene glycol, propylene glycol , glycerin, N-methyl-2-pyrrolidone, N, N-dimethylalkylamides, dimethyl sulfoxide.

[0069] Solid vehicles that can be used in the compositions of the present invention may include, but are not limited to, atapulgite, pyrophyllite clay, silica, kaolin clay, kieselguhr, limestone, diatomaceous earth, lime, calcium carbonate, bentonite clay, earth of Fuller, talc, cottonseed husks, wheat flour, soy flour, pumice stone, wood flour, nutshell flour, lignin, cellulose, etc.

[0070] Thickeners such as silicates, such as metal silicates, carboxymethyl cellulose, methyl cellulose, ethyl cellulose, polyvinyl alcohol, sodium alginate, sodium polyacrylate, xanthan gum, welana gum, arabic gum, montmorillonite, lignosulfonates, hydroxy methyl cellulose, hydroxyl methyl cellulose, hydroxyl methyl cellulose, hydroxyl methyl cellulose, hydroxyl methyl cellulose, hydroxyl methyl cellulose, hydroxyl methyl cellulose, hydroxyl methyl cellulose, hydroxyl methyl cellulose, hydroxyl methyl cellulose. starch and mixtures thereof;

[0071] Anti-freezing agents such as glycerin, ethylene glycol, propylene glycol, preferably propylene glycol and the like. Defoaming agents such as silicone oils, mineral oils, fatty acid ester; biocides such as sodium benzoate, 1,2-benzisothiazolin-3-one, 2-methyl-4-isothiazolon3-one, 5-chloro-2-methyl-4-isothiazolin-3-one, potassium sorbate, para-hydroxybenzoates and similar. Pigments can also be included in the formulation and may include, but are not limited to, blue pigment 15: 4, blue pigment 15: 3, blue pigment 15: 2, blue pigment 15: 1, blue pigment 80, yellow pigment 1, yellow pigment 13, red pigment 112, red pigment 48: 2, red pigment 48: 1, red pigment 57: 1, red pigment 53: 1,

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16/28 orange pigment 43, orange pigment 34, orange pigment 5, green pigment 36, green pigment 7, white pigment 6, brown pigment 25, basic violet 10, basic violet 49, violet 23, acid red 51, acid red 52, acid red 14, acid blue 9, acid yellow 23, basic red 10, basic red 108.

[0072] Polymers such as polyvinyl alcohols, polyvinyl pyrrolidone, gel-forming carrageenans, water-soluble gelatin and casein, superabsorbent polymers like polyacrylamides and polyacrylate-based polymers, semi-synthetic superabsorbent polymers or fully synthetic polymers based on protein peptides / proteins collagen base, elastin polypeptides, polyaspartic acid, polyaspartates, polyglutamic acid, polyglutamate; Semi-synthetic or fully synthetic polysaccharides: carboxymethyl starch, sulfoethyl starch, carboxymethyl cellulose, sulfoethyl cellulose, hydroxypropyl cellulose, hydroxyethyl cellulose, methyl cellulose, chitosan; cross-linked polysaccharides as CMS cross-linked with multifunctional carboxylic acids or multifunctional epoxides and the like. Polysaccharide graft copolymer: Polysaccharides obtained by graft polymerization of a monomer into a polysaccharide, the monomer being selected from acrylonitrile, acrylic acid, methacrylic acid, acrylamide, methacrylamide, 2-acrylamido2-methyl-propane, sulfonic acid (AM) vinyl sulphonic acid, ethyl acrylate and potassium acrylate and the like.

[0073] The combination of the present invention is effective against phytopathogenic fungi, which occur especially in plants, including fungi transmitted by seeds and belonging to the following classes, such as those selected from ascomycetes (for example Penicillium, Gaeumannomyces graminis); basidiomycetes (for example the genus Hemileia, Rhizoctonia, Puccinia); imperfect fungi (for example,

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Botrytis,

Helminthosporium,

Rhynchosporium,

Fusarium,

Septoria,

Cercospora,

Alternaria,

Pyricularia

Pseudocercosporella herpotrichoides);

oomycetes for example, Phytophthora,

Peronospora,

Bremia, Pythium,

Plasmopara); zygomycetes (e.g.,

Rhizopus spp.). The combination of the present invention is especially effective against Alternaria spp.,

Ascochyta spp., Aspergillus spp.,

Claviceps purpurea, Cochliobolus spp., Colletotrichum spp.,

Diplodia maydis, Erysiphe graminis, Fusarium spp.

how

Fusarium culmorum, Fusarium oxysporum, Fusarium solani,

Fusarium graminearum and Fusarium moniliforme, Fusarium subglutinans), Gaeumannomyces graminis, Giberella fujikuroi,

Giberella zeae, Helminthosporium spp. (such as Helminthosporium graminearum, Helminthosporium oryzae, Helminthosporium solani), Monographella nivalis,

Penicillium spp., Puccinia spp., Pyrenophora spp.

like Pyrenophora graminea),

Peronosclerospora spp.,

Peronspora spp.,

Phakopsora pachyrhizi, Phythium spp.,

Phoma spp., Phomopsis spp.,

Rhizoctonia spp. (such as Rhizoctonia cerealis, Rhizoctonia solani), Septoria spp., Pseudocercosporella spp., Tilletia spp., Rhizopus spp., Thielaviopsis basicola, Typhula spp.,

Ustilago spp., Sphacelotheca spp. (for example, Spacelotheca reilliani), Thanatephorus cucumeris, and Verticillium spp.

[0074] The present combinations can be used to control insect pests such as those of the order Lepidoptera, pests such as Acleris spp., Adoxophyes spp., Aegeria spp.,

Agrotis spp., Alabama argillaceae, Amylois spp.,

Anticarsia gemmatalis, Archips spp.,

Argyrotaenia spp.,

Autographa spp.,

Busseola beetle,

Cadra cautella,

Carposina nipponensis, Chilo spp.,

Choristoneura spp.,

Clysia ambiguella, Cnaphalocrocis spp., Cnephasia spp., Cochylis spp., Coleophora spp., Crocidolomia spp., Leukotreta, Crysodeixis includens, Cydia

Cryptophlebia spp., Diatraea spp., Diparopsis castanea, Earias spp., Elasmopalpus spp.,

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Ephestia spp., Eucosma spp., Eupoecilia ambiguella, Euproctis spp., Euxoa spp., Grapholita spp., Hedya nubiferana, Helíothís spp., Hellula undalis, Hyphantria cunea, Keiferia lycopersicella, Leucoptera scitella, Lyocolla spit. spp., Lyonetia spp., Malacosoma spp., Mamestra brassicae, Manduca sexta, Operophtera spp., Ostrinia nubilalis, Pammene spp., Pandemis spp., Panolis flammea, Pectinophora gossypiella, Phthorimaea operculella, Pieris rapae, Pieris spyl. , Prays spp., Scirpophaga spp., Sesamia spp., Sparganothis spp., Spodoptera spp., Synanthedon spp., Thaumetopoea spp., Tortrix spp., Trichoplusia ni and Yponomeuta spp .; of the order Coleoptera, for example, Agriotes spp., Anthonomus spp., Atomaria linearis, Ceutorhynchus spp., Chaetocnema tibialis, Cosmopolites spp., Curculio spp., Dermestes spp., Diabrotica spp., Epilachna spp., Eremnus spp., Gonemen spp., Heteronychus spp., Leptinotarsa decemlineata, Lissorhoptrus spp., Melolontha spp., Orycaephilus spp., Otiorhynchus spp., Phlyctinus spp., Phyllotreta spp., Popillia spp., Protostrophus spp., Psyll. Scarabeidae, Sitophilus spp., Sitotroga spp., Tenebrio spp., Tribolium spp. and Trogoderma spp .; of the order Orthoptera, for example, Blatta spp., Blattella spp., Gryllotalpa spp., Leucophaea maderae, Locusta spp., Periplaneta spp. and Schistocerca spp .; of the order Isoptera, for example, Reticulitermes spp .; of the order Psocoptera, for example, ü poscelis spp; of the order Anoplura, for example, Haematopinus spp., Linognathus spp., Pediculus spp., Pemphigus spp. and Phylloxera spp .; of the order Mallophaga, for example, Damalinea spp. and Trichodectes spp .; of the order Thysanoptera, for example, Frankliniella spp., Hercinothrips spp., Taeniothrips spp., Thrips palmi, Thrips tabaci and Scirtothrips aurantii; of the order Heteroptera, for example, Dichelops melacanthus,

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Distantiella theobroma, Dysdercus spp., Euchistus spp.,

Eurygaster spp., Leptocorisa spp., Nezara spp., Piesma spp., Rhodnius spp.,

Sahlbergella singularis

Scotinophara spp. and Triatoma spp .; of the order Homoptera, for example, insects such as Aleurothrixus floccosus, Aleyrodes brassicae,

Aonidiella spp., Aphididae,

Aphis spp., Aspidiotus spp.,

Bemisia tabaci, Ceroplaster spp., Chrysomphalus aonidium,

Chrysomphalus dictyospermi,

Coccus hesperidum,

Empoasca spp., Eriosoma larigerum

Erythroneura spp.,

Gascardia spp., Laodelphax spp.,

Lecanium corni, Lepidosaphes spp.,

Macrosiphus spp.,

Myzus spp., Nephotettix spp., Nilaparvata spp., Paratoria spp.,

Pemphigus spp., Planococcus spp.,

Pseudaulacaspis spp.,

Pseudococcus spp., Psylla spp.,

Pulvinaria aethiopica,

Quadraspidiotus spp.,

Rhopalosiph um spp., Saissetia spp.,

Scaphoideus spp., Schizaphis spp.,

Sitobion spp.,

Trialeurodes vaporariorum, Trioza erytreae and

Unaspis citri; of the order Hymenoptera, for example,

Acromyrmex, Athalia rosae, Atta spp.,

Cephus spp.,

Diprion spp., Diprionidae, Gilpinia polytoma, Hoplocampa spp.,

Lasius spp., Monomorium pharaonis, Neodiprion spp.,

Solenopsis spp. and Vespa spp .; of the order Diptera, for example, Antherigona soccata, Bibio hortulanus, Ceratitis spp., Chrysomyia spp., Culex spp., Cuterebra spp., Dacus spp., Delia spp.,

Drosophila melanogaster, Liriomyza spp.,

Melanagromyza spp.,

Orseolia spp.,

Oscinella frit, Pegomyia hyoscyami,

Phorbia spp., Rhagoletis pomonella

Sciara spp.,; of the order Acarina, for example, Acarus siro,

Aceria sheldoni,

Aculus schlechtendali

Amblyomma spp.,

Argas spp., Brevipalpus spp.,

Bryobia praetiosa, Calipitrimerus spp., Chorioptes spp., Dermanyssus gallinae, Eotetranychus carpini, Eriophyes spp., Hyalomma spp., Olygonychus pratensis, Ornithodoros spp., Panonychus spp., Phyllocoptrute spp., Latvia, Polypha Rhizoglyphus spp.,

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Sarcoptes spp.,

Tarsonemus spp. and Tetranychus spp .; and from the Nematoda class, for example, the Meloidogyne spp.

for example,

Meloidogyne incoginita and Meloidogyne javanica),

Heterodera spp.

for example,

Heterodera glycines,

Heterodera schachtii,

Heterodora avenae e

Heterodora trifolii),

Globodera spp. (for example,

Globodera rostochiensis), Radopholus spp. (for example,

Radopholus similes), Rotylenchulus spp., Pratylenchus spp.

(for example, Pratylenchus neglectans and Pratylenchus penetrans), Aphelenchoides spp., Helicotylenchus spp., Hoplolaimus spp., Paratrichodorus spp., Longidorus spp., Nacobbus spp., Subanguina spp. Belonlaimus spp., Criconemella spp., Criconemoides spp., Ditylenchus spp., Dolichodorus spp., Hemicriconemoides spp., Hemicycliophora spp., Hirschmaniella spp., Hypsoperine spp., Macroposthonia spp., Meliniodus spp., Punctual ., Scutellonema spp., Xiphinema spp. and Tylenchorhynchus spp.

[0075] In one embodiment, the combination of the present invention can be used to treat plant propagation materials from crops such as cereals, such as wheat, barley, rye, oats, corn, rice, sorghum, triticale and related crops; beet, such as sugar beet and

beet forage; leguminous plants such as beans, Lentil, peas, soybeans; oil plants, such as rapeseed, mustard and sunflower; cucurbits, such as zucchinis, cucumbers, melons; fibrous plants, such as cotton, flax,

hemp, jute; vegetables such as spinach, lettuce, asparagus, cabbage, carrots, onions, tomatoes, potatoes, paprika, as well as ornamental plants such as flowers, shrubs, broad-leaved and persistent trees, such as conifers. Plant propagation material can also be used in varieties of plant propagation material, such as conventional, hybrid or transgenic plants.

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21/28 [0076] In one embodiment, the combinations of the present invention may also contain herbicides, fungicides, insecticides, fertilizers, biological agents, other plant additives such as mycorrhiza, silicic acid, growth promoters derived from plants etc.

[0077] In one aspect, the present invention can provide methods for improving the vigor or productivity of the plant, comprising applying to a plant propagating material, before sowing, a combination comprising:

i) fipronil;

ii) azoxystrobin;

iii) a benzimidazole fungicide selected from carbendazim or methyl thiophanate; and iv) an active agent selected from imidacloprid, acetamiprid, thiamethoxam, or a multisite fungicide, or both.

[0078] In one embodiment, the multisite fungicide is a dithiocarbamate fungicide.

[0079] In one embodiment, the dithiocarbamate fungicide is mancozeb.

[0080] In one embodiment, the multisite fungicide is a phthalimide fungicide selected from captana, captafol and folpet.

[0081] In one modality, the multisite fungicide it is a fungicide in chloronitrile, as chlorothalonil. [0082] In one modality, the multisite fungicide it is a

sulfamide fungicide selected from diclofluanide and tolylfluanide.

[0083] In one embodiment, the multisite fungicide is a bis-guanidine fungicide selected from guazatin and iminoctadine.

[0084] In one embodiment, the multisite fungicide is a triazine fungicide selected from anilazine.

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22/28 [0085] In one embodiment, the multisite fungicide is a quinone fungicide selected from dithianone.

[0086] In one embodiment, the multisite fungicide is a quinoxaline fungicide and selected from quinomethionate and chloroquine.

[0087] In one embodiment, the multisite fungicide is a dicarboxamide fungicide selected from fluoroimide.

[0088] In one embodiment, the multisite fungicide is an inorganic fungicide selected from copper fungicides, including copper (II) hydroxide, copper oxychloride, copper (II) sulfate, basic copper sulfate, Bordeaux mixture, copper salicylate , C ₇ H ₄ O ₃ * Cu, CU ₂ O cuprous oxide; or sulfur.

[0089] In another aspect, the present invention can provide methods to control fungi and insect pests transmitted by the soil, the method comprising applying to a plant propagating material, before sowing, a combination comprising:

i) fipronil;

ii) azoxystrobin;

iii) a benzimidazole fungicide selected from carbendazim or methyl thiophanate; and iv) an active ingredient selected from imidacloprid, acetamipride, thiamethoxam, mancozebe, manebe, zineb, folpete, chloratalonyl and tribasic copper sulfate or multisite fungicide, or both.

[0090] In one embodiment, the multisite fungicide is a dithiocarbamate fungicide.

[0091] In one embodiment, the dithiocarbamate fungicide is mancozeb.

[0092] In one embodiment, the multisite fungicide is a phthalimide fungicide selected from captana, captafol and folpet.

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[0093] In one modality, the multisite fungicide is one fungicide in chloronitrile, as chlorothalonil. [0094] In one modality, the multisite fungicide is one fungicide in sulfamide selected from diclofluanide and

tolylfluanide.

[0095] In one modality, the fungicide multisite is one fungicide bis-guanidine selected among guazatina and iminoctadine. [0096] In one modality, the fungicide multisite is one fungicide indigo selected triazine azine. [0097] In one modality, the fungicide multisite is one fungicide quinone selected from dithianone. [0098] In one modality, the fungicide multisite is one

quinoxaline fungicide selected from quinomethionate and chloroquine.

[0099] In one embodiment, the multisite fungicide is a dicarboxamide fungicide selected from fluoroimide.

[0100] In one embodiment, the multisite fungicide is an inorganic fungicide selected from copper fungicides, including copper (II) hydroxide, copper oxychloride, copper (II) sulfate, basic copper sulfate, Bordeaux mixture, copper salicylate , C ₇ H ₄ O ₃ * Cu, cuprous oxide Cu2O; or sulfur.

[0101] In one aspect, the combination of the present invention can be presented in the form of a kit of parts, or in the form of a ready-mix formulation that can be readily applied to plant propagating material or that can be applied to the plant propagation material before being sold commercially.

[0102] Surprisingly, the present inventors found that combinations of fipronil, azoxystrobin, a benzimidazole fungicide and at least one among imidacloprid or a multisite fungicide, when applied individually, were ineffective in controlling

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24/28 fungi and insect pests transmitted by the soil, but demonstrated excellent synergistic control over weeds, when applied together. The combination controlled the fungi and insect pests transmitted by the soil. The combination of fipronil, azoxystrobin, a benzimidazole fungicide and at least one among imidacloprid or a multisite fungicide controlled for diseases transmitted by the seed, such as seed rot, among others. The present invention therefore provides advantageous methods against fungi and insect pests transmitted by the soil. The present method also provides a broader spectrum of control of such pests and helps in the management of resistance, thus improving the germination rate and the production of plants with improved vigor and a broader spectrum of control at lower rates of use.

Advantages of the present invention:

[0103] The combinations of the present invention have at least one of the following advantages:

[0104] These combinations prevent mycelial development, spore germination and the formation of appressoria and haustoria, controlling the main soil and seed pathogens, such as Rhizoctonia solani and Sclerotinia sclerotiorum. It was found that the combinations were totally selective for the crops and, not interfering in the organisms responsible for nodulation in vegetables, containing the first multisite fungicide for seed treatment that has multiple action in relation to the cellular functions of pathogens, form a barrier on the seed surface. The combinations of the invention thus eliminated the fungi present on the surface of the seeds and prevented the germination of the spores that appeared after the treatment, presenting a synergistic effect between the three fungicides, enhanced by the mancozebe.

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25/28 [0105] It was found that the storage time of the seeds thus treated is greater than 120 days without interfering with the germination and vigor patterns.

[0106] The combination of the invention could be adapted to a violet color associated with optical brightness, providing nighttime glow by means of black light, making it difficult to falsify the product and assisting in the differentiation between grain and treated seed.

[0107]

The combinations exhibited an improvement in the plant's defense mechanism against pathogens. They promoted the growth of seedlings by increasing the root system, with greater depth of the roots and better use of nutrients.

Increased photosynthesis, consequently less energy consumption of the seedling, providing vigorous growth, conditioning the plant to achieve superior productivity even under stress conditions.

[0108] The combinations of the invention facilitated a better coverage of the seeds due to the adhesion products through a differentiated and specific formulation for the treatment of the seeds and, consequently less loss of the active ingredient.

Example:

[0109] Experiments were carried out to study the combinations of the present invention for the treatment of seeds in corn. These studies aimed to verify the effectiveness of doses of combinations of the inventions compared to standard products. The following combinations were mixed in a tank and used to treat corn seeds.

Serial No. Composition Combination components 1 UPL1 Fipronil 150 g / L + Imidacloprid 150 g / L + Azoxystrobin 30 g / L + Cabendazime 90 g / L 2 UPL2 Fipronil 150 g / L + Imidacloprid 150 g / L + Azoxystrobin 30 g / L + Thiophanate-methyl 120 g / L

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3 UPL3 Fipronil 160 g / L + Mancozebe 160 g / L + Azoxystrobin 23 g / L + Thiophanate-methyl 90 g / L 4 Comparative P1 Metalaxyl 20 g / L + Thiabendazole 150 g / L + Fludioxonil 25 g / L 5 Comparative P2 Thiamethoxam 350 g / L 6 Comparative P3 Tiodicarb 450 g / L + Imidacloprid 150 g / L 7 Comparative P4 Clothianidin 600 g / L

[0110] These combinations were used to treat corn seeds before sowing, and the treated seeds were tested for plant resistance after emergence. The resistance of the plant was tested by measuring the percentage of plants found lying (fallen) 70 days after emergence (DAE) in the field. The following results were tabulated:

Table 1:

Serial No. Treatment Fallen plants (percentage (%)) at 70 DAE 1 Untreated 77.5 2 UPL1 at 0.30 kg / Ha 7.6 3 UPL1 at 0.50 kg / Ha 6.6 4 UPL2 at 0.40 kg / Ha 1.2 5 UPL3 at 0.30 kg / Ha 0.0 6 UPL3 at 0.40 g / Ha 10.0 7 UPL3 at 0.50 kg / Ha 7.5 8 P3 + Carbendazime 150 g / L + Thyme 350 g / L 42.5 9 P1 + P2 + Fipronil at 250 g / L 61.2 10 Pyroclostrobin 25 g / L + Methyl thiophanate 225 g / L + Fipronil 250 g / L 12.5

[0111] It can be concluded, then, that plants emerging from seeds treated with the combinations according to the present invention showed surprisingly improved plant resistance.

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Example 2:

[0112] The UPL2 combination according to the present invention was tested for the advantages of seed treatment in dry beans, and compared to the industry standard of thiadicarb 225 g / L and imidacloprid 75 g / L. Dry bean seeds were treated with the following regime, and the percentage of Bemisia tabaci nymph control was calculated using Abbott's formula. The following results were tabulated:

Table 2:

Serial No. Treatment [/ per 100 kg of seeds] Bemisia tabaci Control (%) of nymphs Abbott formula 7 DAE 15 DAE 21 DAE 3 0 AED 4 5 DAE 1 Untreated 0.0 0.0 0.0 0.0 0.0 2 UPL2 at 0.40 kg 80.2 66.3 39.4 68.8 38.1 3 UPL2 at 0.50 kg 81.0 73.6 33.3 25.0 33.3 4 UPL2 at 0.60 kg 94.3 89.6 42.4 62.5 23.8 5 UPL2 at 0.70 kg 89.1 82.8 27.3 72.5 66.7 6 Thiadicarb 225 g / L and imidacloprid 75 g / L [at 0.50 kg] 54.9 54.7 15.2 37.5 4.7

[0113] The control percentage (%) for Bemisia tabaci adults and nymphs was also calculated using Abbott's formula and tabulated below:

Table 3:

Serial No. Treatment [/ per 100 kg of seeds] Bemisia tabaci Control (%) of nymphs + adults Abbott Formula 7 DAE 15 DAE 21 DAE 3 0 AED 4 5 DAE 1 Untreated 0.0 0.0 0.0 0.0 0.0 2 UPL2 at 0.40 kg 80.2 66.3 84.4 49.7 0.0 3 UPL2 at 0.50 kg 81.0 73.6 30.5 21.7 15.4 4 UPL2 at 0.60 kg 94.3 89.6 77.3 63.7 40.0 5 UPL2 at 0.70 kg 89.1 82.8 81.3 70.1 44.6 6 Thiadicarb 225 g / L e imidaclopride 75 54.9 54.7 11.7 0.6 0.0

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g / L [at 0.50 kg]

[0114] It was concluded, therefore, that the combinations of the present invention were surprisingly effective for the control of Bemisia tabaci nymphs and adults during the treatment of dry bean seeds.

[0115] Although the aforementioned written description of the invention allows a person skilled in the art to make and use what is presently considered to be the best mode of the same, those skilled in the art will understand and recognize the existence of variations, combinations and equivalents of the modality, of the method and specific examples of the present invention. The invention, therefore, should not be limited by the modality, method and examples described above, but by all modalities and methods within the scope and spirit of the invention.

Claims (11)

1. Combination for seed treatment, characterized by the fact that it comprises: The. fipronil; B. azoxystrobin; ç. a benzimidazole fungicide; and d. at least one active agent selected from a neonicotinoid insecticide or a multisite fungicide, or both. 2. Combination according to claim 1, characterized by the fact that the benzimidazole fungicide is selected from albendazole, benomile, carbendazim, chlorphenazole, cipendazole, debacarb, fuberidazole, mecarbinzide, rabenzazole, thiabendazole, furofanate, thiophanate and thiophanate. 3. Combination, according to claim 1, characterized by the fact that the multisite fungicide is a fungicide selected from the group consisting of dithiocarbamates, phthalimides, chloronitriles, inorganic fungicides, sulfamides, bis-guanidines, triazines, quinones, quinoxalins and dicarboxamides. 4. Combination, according to claim 1, characterized by the fact that the dithiocarbamate fungicide is selected from asamobam, asomato, azithiram, carbamorph, cufranebe, cuprobame, disulfirame, ferbame, metame, nabame, tirame, urbacida, zirame, dazomete , eteme, milnebe, tecorame, mancobre, mancozebe, manebe, metirame, polycarbamate, propineb and zineb; the phthalimide fungicide is selected from captana, captafol and folpete; the chloronitrile fungicide is chlorothalonil; the sulfamide fungicide is selected from diclofluanide and tolylfluanide; the bis-guanidine fungicide is selected from guazatin and iminoctadine; Petition 870190004010, of 01/14/2019, p. 127/130 2/3 the multisite fungicide is a triazine fungicide selected from anilazine; the multisite fungicide is a quinone dithianone fungicide; the multisite fungicide is a quinoxaline fungicide selected from quinomethionate and chloroquinox is a dicarboxamide fungicide selected from fluoroimide; an inorganic fungicide selected from copper fungicides, including copper (II) hydroxide, copper oxychloride, copper (II) sulphate, basic copper sulphate, Bordeaux mixture, copper salicylate C7H4O3 * Cu, cuprous oxide CU2O; or sulfur. 5. Combination according to claim 1 characterized by the fact that the neonicotinoid insecticide can be selected from the group consisting of acetamiprid, clothianidin, imidacloprid, nitenpyram, nitiazine, thiaclopride and thiamethoxam. 6. Combination, according to claim 1, said combination being characterized by the fact that it comprises: The. fipronil, azoxyestrobin, carbendazim; and mancozebe; B. fipronil, azoxyestrobin, carbendazim; and imidaclopride; fipronil, azoxyestrobin, methyl thiophanate; and mancozebe; d. fipronil, azoxyestrobin, methyl thiophanate; imidaclopride; and. fipronil, azoxyestrobin, carbendazim; and acetamipride; f. fipronil, azoxyestrobin, methyl thiophanate; acetamipride; g. fipronil, azoxyestrobin, carbendazim; and thiamethoxam; H. fipronil, azoxyestrobin, methyl thiophanate; thiamethoxam; i. fipronil, azoxyestrobin, carbendazim; and chlorothalonil; j. fipronil, azoxyestrobin, methyl thiophanate; chlorothalonil; Petition 870190004010, of 01/14/2019, p. 128/130 3/3 k. fipronil, azoxyestrobin, carbendazim; and tribasic copper sulfate; l. fipronil, azoxyestrobin, methyl thiophanate; and tribasic copper sulfate. 7. Combination, according to claim 1, said combination being characterized by the fact that it is mixed by addition in the ratio of (1 to 80) :( 1 to 80) :( 1 to 80) :( 1 to 80). 8. Composition, characterized by the fact that it comprises a combination as defined in claims 1 to 7. 9. Composition, according to claim 8, characterized by the fact that fipronil is present in the range of 0.1 to 99%, by weight; azoxystrobin is present in the range of 0.1 to 99%, by weight; the benzimidazole inhibitor is present in the range of 0.1% to 99%, by weight; neonicotinoid is present in the range of 0.1 to 99% by weight, or the multisite fungicide is present in the range of 0.1 to 99% by weight. 10. Method to improve the vigor or productivity of the plant, characterized by the fact of understanding to apply to the plant propagation material, before sowing, a combination as defined in claims 1 to 8. 11. Method for to control fungus pests and insects transmitted fur ground, characterized by fact in comprising applying to a plant propagating material, prior to sowing, a combination comprising a combination as defined in claims 1 to 8. 12. Kit, characterized by the fact that it comprises a kit of parts or a ready-mix formulation that can be readily applied to plant propagation material, or can be applied to plant propagation material in the form of a seed treatment combination , as claimed in claims 1 to 8.