BR0317765A - Artificial promoter, DNA fragment from an artificial promoter, cassette, DNA vector, bacterial cell, plant cell, transgene plant, plant progeny, and purification and use of a recombinant protein - Google Patents

Artificial promoter, DNA fragment from an artificial promoter, cassette, DNA vector, bacterial cell, plant cell, transgene plant, plant progeny, and purification and use of a recombinant protein

Info

Publication number
BR0317765A
BR0317765A BR0317765-3A BR0317765A BR0317765A BR 0317765 A BR0317765 A BR 0317765A BR 0317765 A BR0317765 A BR 0317765A BR 0317765 A BR0317765 A BR 0317765A
Authority
BR
Brazil
Prior art keywords
plant
promoter
dna
artificial
artificial promoter
Prior art date
Application number
BR0317765-3A
Other languages
Portuguese (pt)
Inventor
Guillermo Selman-Housein Sosa
Alberto Salazar Rodriguez
Daymi Abreu Remedios
Osmany Ramos Gonzalez
Original Assignee
Ct Ingenieria Genetica Biotech
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=40293792&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=BR0317765(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Ct Ingenieria Genetica Biotech filed Critical Ct Ingenieria Genetica Biotech
Publication of BR0317765A publication Critical patent/BR0317765A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

"PROMOTOR ARTIFICIAL, FRAGMENTO DE DNA DE UM PROMOTOR ARTIFICIAL, CASSETE, VETOR DE DNA, CéLULA BACTERIANA, CéLULA VEGETAL, PLANTA TRANSGêNCIA, DESCENDêNCIA DE PLANTAS, E, PURIFICAçãO E UTILIZAçãO DE UMA PROTEìNA RECOMBINANTE". A invenção diz respeito a um promotor artificial que é caracterizado em que compreende uma molécula quimérica de DNA recombinante, o qual, uma vez introduzido nas células de plantas de qualquer classe, promove altos níveis de expressão de qualquer molécula de DNA que seja fundida à sua extremidade 3<39>. Os elementos genéticos básicos da molécula do promotor da invenção são como a seguir: um núcleo de promotor com uma TATA-boxe de consenso seguida por uma região éxon/ìntron/éxon e um elemento de potenciação da atividade translacional, todos dos quais sendo produzidos artificialmente. Os elementos reguladores da expressão transcricional podem ser introduzidos a montante do promotor de modo a fornecer a expressão com a capacidade de resposta temporal específica de órgão ou tecido. Os elementos genéticos artificiais projetados podem ser funcionalmente introduzidos entre qualquer promotor ativo nas células de plantas e qualquer seq³ência de DNA, de modo a aumentar os níveis de transcrição/tradução desta última."ARTIFICIAL PROMOTER, DNA FRAGMENT OF AN ARTIFICIAL PROMOTER, CASSETTE, DNA VECTOR, BACTERIAL CELL, VEGETABLE CELL, PLANT PLANT, AND PURIFICATION AND USE OF A RECOMMENDATION. The invention relates to an artificial promoter which comprises a recombinant DNA chimeric molecule which, once introduced into plant cells of any class, promotes high levels of expression of any DNA molecule that is fused to its structure. end 3 <39>. The basic genetic elements of the promoter molecule of the invention are as follows: a promoter nucleus with a consensus TATA box followed by an exon / intron / exon region and a translational activity enhancing element, all of which are artificially produced . Regulatory elements of transcriptional expression may be introduced upstream of the promoter to provide expression with organ or tissue specific temporal responsiveness. Engineered artificial genetic elements can be functionally introduced between any active promoter in plant cells and any DNA sequence in order to increase transcription / translation levels of the latter.

BR0317765-3A 2002-12-27 2003-12-19 Artificial promoter, DNA fragment from an artificial promoter, cassette, DNA vector, bacterial cell, plant cell, transgene plant, plant progeny, and purification and use of a recombinant protein BR0317765A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CU20020337 2002-12-27
PCT/CU2003/000018 WO2004058979A1 (en) 2002-12-27 2003-12-19 Artificial promoter for the expression of dna sequences in vegetal cells

Publications (1)

Publication Number Publication Date
BR0317765A true BR0317765A (en) 2005-11-22

Family

ID=40293792

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0317765-3A BR0317765A (en) 2002-12-27 2003-12-19 Artificial promoter, DNA fragment from an artificial promoter, cassette, DNA vector, bacterial cell, plant cell, transgene plant, plant progeny, and purification and use of a recombinant protein

Country Status (13)

Country Link
US (1) US20060277628A1 (en)
EP (1) EP1577395A1 (en)
JP (1) JP2006512067A (en)
KR (1) KR20050090411A (en)
CN (1) CN1738902A (en)
AR (1) AR042552A1 (en)
AU (1) AU2003291914A1 (en)
BR (1) BR0317765A (en)
CA (1) CA2518966A1 (en)
MX (1) MXPA05006983A (en)
RU (1) RU2326167C2 (en)
WO (1) WO2004058979A1 (en)
ZA (1) ZA200505067B (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2118287B1 (en) * 2007-01-05 2014-11-05 Metahelix Life Sciences Private Limited A chimeric promoter and a method thereof
JP5158639B2 (en) 2008-04-11 2013-03-06 独立行政法人農業生物資源研究所 Genes specifically expressed in the endosperm of plants, promoters of the genes, and use thereof
JP5594683B2 (en) * 2010-03-19 2014-09-24 国立大学法人静岡大学 promoter
RU2644205C2 (en) * 2011-03-25 2018-02-08 Монсанто Текнолоджи Ллс Plant regulatory elements and their application
MX352668B (en) * 2012-12-19 2017-12-01 Monsanto Technology Llc Plant regulatory elements and uses thereof.
CA2903226C (en) * 2013-03-15 2022-03-15 Bayer Cropscience Lp Constitutive soybean promoters
RU2699982C2 (en) * 2014-01-10 2019-09-11 Медикаго Инк. Enhancer elements cpmv
JP2023015421A (en) * 2020-01-10 2023-02-01 日本たばこ産業株式会社 enhancer

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ZA88319B (en) * 1987-02-06 1988-08-12 Lubrizol Enterprises, Inc. Ocs enhancer
WO1991009948A1 (en) * 1990-01-05 1991-07-11 Cornell Research Foundation, Inc. Rice actin gene and promoter
US5641876A (en) * 1990-01-05 1997-06-24 Cornell Research Foundation, Inc. Rice actin gene and promoter
DE4222407C1 (en) * 1992-07-08 1993-10-07 Max Planck Gesellschaft Modular promoter construct
US6072050A (en) * 1996-06-11 2000-06-06 Pioneer Hi-Bred International, Inc. Synthetic promoters
AU729929B2 (en) * 1996-06-11 2001-02-15 Pioneer Hi-Bred International, Inc. A synthetic plant core promoter and upstream regulatory element
WO1998010081A2 (en) * 1996-09-03 1998-03-12 Plant Genetic Systems, N.V. Improved barstar-gene
US6573438B1 (en) * 1997-03-14 2003-06-03 Syngenta Participations Ag Inbred Maize line 2044BT
US6346655B1 (en) * 1999-03-31 2002-02-12 Syngenta Participations Ag Trichothecne-Resistant transgenic plants

Also Published As

Publication number Publication date
KR20050090411A (en) 2005-09-13
AU2003291914A1 (en) 2004-07-22
US20060277628A1 (en) 2006-12-07
EP1577395A1 (en) 2005-09-21
CN1738902A (en) 2006-02-22
MXPA05006983A (en) 2006-02-22
RU2005123815A (en) 2006-01-27
RU2326167C2 (en) 2008-06-10
JP2006512067A (en) 2006-04-13
CA2518966A1 (en) 2004-07-15
ZA200505067B (en) 2006-05-31
AR042552A1 (en) 2005-06-22
WO2004058979A1 (en) 2004-07-15

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Legal Events

Date Code Title Description
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE A 7A ANUI DADE.

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 2112 DE 28/06/2011.