BR0303224A - Detecção de nucleotìdeos por transferência de energia de ressonância fluorescente e métodos para a mesma - Google Patents

Detecção de nucleotìdeos por transferência de energia de ressonância fluorescente e métodos para a mesma

Info

Publication number
BR0303224A
BR0303224A BR0303224-8A BR0303224A BR0303224A BR 0303224 A BR0303224 A BR 0303224A BR 0303224 A BR0303224 A BR 0303224A BR 0303224 A BR0303224 A BR 0303224A
Authority
BR
Brazil
Prior art keywords
methods
energy transfer
resonance energy
fluorescent resonance
nucleotide
Prior art date
Application number
BR0303224-8A
Other languages
English (en)
Inventor
Gerardo Turcatti
Original Assignee
Applied Research Systems
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Applied Research Systems filed Critical Applied Research Systems
Publication of BR0303224A publication Critical patent/BR0303224A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

"DETECçãO DE NUCLEOTìDEOS POR TRANSFERêNCIA DE ENERGIA DE RESSONâNCIA FLUORESCENTE E MéTODOS PARA A MESMA". A presente invenção fornece novos processos para a detecção da incorporação de um nucleotídeo após uma reação de extensão de bases isoladas. Os processos fazem uso do efeito da Transferência de Energia de Ressonância Fluorescente (FRET) gerado por dois fluoróforos ligados ao mesmo filamento de ácido nucléico. Os processos podem ser utilizados, em solução ou em um suporte sólido, para aplicações que requerem a identificação rápida e precisa de um nucleotídeo em uma posição específica de um ácido nucléico, como para estudos de genotipagem.
BR0303224-8A 2002-02-11 2003-02-07 Detecção de nucleotìdeos por transferência de energia de ressonância fluorescente e métodos para a mesma BR0303224A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP02100124 2002-02-11
PCT/EP2003/050013 WO2003068986A1 (en) 2002-02-11 2003-02-07 Nucleotide detection by fluorescence resonance energy transfer and methods thereof

Publications (1)

Publication Number Publication Date
BR0303224A true BR0303224A (pt) 2004-07-06

Family

ID=27675737

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0303224-8A BR0303224A (pt) 2002-02-11 2003-02-07 Detecção de nucleotìdeos por transferência de energia de ressonância fluorescente e métodos para a mesma

Country Status (4)

Country Link
AU (1) AU2003226829A1 (pt)
BR (1) BR0303224A (pt)
NO (1) NO20034592L (pt)
WO (1) WO2003068986A1 (pt)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0324456D0 (en) * 2003-10-20 2003-11-19 Isis Innovation Parallel DNA sequencing methods
WO2008002659A2 (en) * 2006-06-29 2008-01-03 Cdex, Inc. Methods and apparatus for molecular species detection, inspection and classification using ultraviolet to near infrared enhanced photoemission spectroscopy

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1273552A (en) * 1985-12-23 1990-09-04 Michael J. Heller Fluorescent stokes shift probes for polynucleotide hybridization assays
JP3439221B2 (ja) * 1995-12-18 2003-08-25 ワシントン ユニヴァーシティ 蛍光共鳴エネルギー移動を利用する核酸分析方法
DE60143974D1 (de) * 2000-03-29 2011-03-10 Lgc Ltd Hybridisierungsprobe und Methode zum schnellen Nachweis und zur schnellen Unterscheidung von Sequenzen

Also Published As

Publication number Publication date
WO2003068986A1 (en) 2003-08-21
NO20034592D0 (no) 2003-10-13
NO20034592L (no) 2003-12-09
AU2003226829A1 (en) 2003-09-04

Similar Documents

Publication Publication Date Title
Wu et al. INSIGHT: A population-scale COVID-19 testing strategy combining point-of-care diagnosis with centralized high-throughput sequencing
Berndl et al. Comparison of a nucleosidic vs non-nucleosidic postsynthetic “click” modification of DNA with base-labile fluorescent probes
Hsiao et al. Methyltransferase-Glo: a universal, bioluminescent and homogenous assay for monitoring all classes of methyltransferases
Laszlo et al. Detection and mapping of 5-methylcytosine and 5-hydroxymethylcytosine with nanopore MspA
Schallig et al. Molecular biological applications in the diagnosis and control of leishmaniasis and parasite identification
EP1746170A3 (en) Probes and methods for hepatitis C virus typing using multidimensional probe analysis
BR0214683A (pt) Molécula de nucleotìdeo ou de nucleosìdeo, métodos de rotular uma molécula de ácido nucléico e para determinar a sequência de um polinucleotìdeo alvo de filamento único, e, kit
Li et al. Specific nucleic acid detection based on fluorescent light-up probe from fluorogens with aggregation-induced emission characteristics
Ylihärsilä et al. Oligonucleotide array-in-well platform for detection and genotyping human adenoviruses by utilizing upconverting phosphor label technology
ATE305476T1 (de) Met/fret markierte amplifikationsoligonukleotide und ihre vewendung in verfahren
BR0206747A (pt) Polinucleotìdeos para uso como etiquetas e complementos de etiquetas, fabricação e uso dos mesmos
Chen et al. Detection of foot-and-mouth disease virus RNA by reverse transcription loop-mediated isothermal amplification
DE69233553D1 (de) Dns "typing" mit kurzen tandem-repetitiven polimorphismen und identifikation von kurzen polimorphen tandem-wiederholungen
CN107760762A (zh) 一种检测dna腺嘌呤甲基转移酶的荧光化学传感器及其检测方法
CA2814552A1 (en) Luminophore-labeled molecules coupled with particles for microarray-based assays
BR0212773A (pt) Métodos para detecção de sequências-alvo de ácido nucléico com marcadores diagnósticos
BRPI0414422A (pt) método para a análise de assinatura de identificação de gene (gis)
Hickey et al. Structure-guided design of fluorescent S-adenosylmethionine analogs for a high-throughput screen to target SAM-I riboswitch RNAs
DE60235005D1 (de) Nachweis der instabilität von mikrosatelliten und
Cheong et al. A fluorescent methylation-switchable probe for highly sensitive analysis of FTO N 6-methyladenosine demethylase activity in cells
BR0309231A (pt) Bandeja para ensaio biológico termicamente condutora, e, método para fabricação da mesma
Hindson et al. Diagnostic evaluation of multiplexed reverse transcription-PCR microsphere array assay for detection of foot-and-mouth and look-alike disease viruses
BR0303224A (pt) Detecção de nucleotìdeos por transferência de energia de ressonância fluorescente e métodos para a mesma
Sýkorová et al. Polymerase synthesis of DNA containing iodinated pyrimidine or 7‐deazapurine nucleobases and their post‐synthetic modifications through the Suzuki‐Miyaura cross‐coupling reactions
ATE484747T1 (de) Teststück fur proteinassay

Legal Events

Date Code Title Description
B11A Dismissal acc. art.33 of ipl - examination not requested within 36 months of filing
B11Y Definitive dismissal - extension of time limit for request of examination expired [chapter 11.1.1 patent gazette]