BR0110289A - Method for determining amylase concentrations - Google Patents

Method for determining amylase concentrations

Info

Publication number
BR0110289A
BR0110289A BR0110289-3A BR0110289A BR0110289A BR 0110289 A BR0110289 A BR 0110289A BR 0110289 A BR0110289 A BR 0110289A BR 0110289 A BR0110289 A BR 0110289A
Authority
BR
Brazil
Prior art keywords
sample
amylase
concentrations
mixture
concentration
Prior art date
Application number
BR0110289-3A
Other languages
Portuguese (pt)
Inventor
Roger Dohmen
Winfried Linxweiler
Original Assignee
Johnson Diversey Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Johnson Diversey Inc filed Critical Johnson Diversey Inc
Publication of BR0110289A publication Critical patent/BR0110289A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/40—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving amylase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)

Abstract

"MéTODO DE DETERMINAçãO DAS CONCENTRAçõES DE AMILASE". Método para a determinação das concentrações de amilase é proporcionado, compreendendo as etapas de: (a) incubar uma parte da amostra possuindo uma concentração desconhecida de amilase a uma temperatura especificada em uma solução de amido tamponada miscível em água contendo amido a uma concentração entre 20-200 mg/litro; (b) deixar a mistura obtida desse modo reagir durante um período especificado de tempo; (c) acrescentar uma parcela de uma solução aquosa de iodo à essa mistura para se obter uma primeira amostra; (d) preparar uma segunda amostra mediante a adição de outra parte da amostra a uma mistura compreendendo uma segunda parcela da solução de iodo e uma segunda parcela da solução de amido; (e) medir a densidade ou a remissão ótica de ambas as amostras a um comprimento de onda entre 300-800 nm; e (f) calcular a atividade amilase na amostra usando uma curva de calibração; onde ambas a primeira e a segunda amostras contêm concentrações iguais da amostra e da solução de amido, e onde o tempo necessário para a realização do método é menor que 10 minutos, preferivelmente menos de 6 minutos. Esse método é adequado para o monitoramento da concentração de amilase em processos industriais de limpeza."METHOD OF DETERMINING AMYLASE CONCENTRATIONS". A method for determining amylase concentrations is provided, comprising the steps of: (a) incubating a portion of the sample having an unknown concentration of amylase at a specified temperature in a starch-containing water-miscible buffered starch solution at a concentration between -200 mg / liter; (b) allowing the mixture thus obtained to react for a specified period of time; (c) adding a portion of an aqueous iodine solution to that mixture to obtain a first sample; (d) preparing a second sample by adding another part of the sample to a mixture comprising a second portion of the iodine solution and a second portion of the starch solution; (e) measuring the density or optical remission of both samples at a wavelength between 300-800 nm; and (f) calculate amylase activity in the sample using a calibration curve; wherein both the first and second samples contain equal concentrations of the sample and starch solution, and where the time required to perform the method is less than 10 minutes, preferably less than 6 minutes. This method is suitable for monitoring amylase concentration in industrial cleaning processes.

BR0110289-3A 2000-04-26 2001-04-17 Method for determining amylase concentrations BR0110289A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP00201500 2000-04-26
PCT/EP2001/004360 WO2001081617A1 (en) 2000-04-26 2001-04-17 Method of determining amylase concentrations

Publications (1)

Publication Number Publication Date
BR0110289A true BR0110289A (en) 2003-02-18

Family

ID=8171410

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0110289-3A BR0110289A (en) 2000-04-26 2001-04-17 Method for determining amylase concentrations

Country Status (7)

Country Link
EP (1) EP1278888A1 (en)
JP (1) JP2003530891A (en)
AU (1) AU2001260219A1 (en)
BR (1) BR0110289A (en)
CA (1) CA2407563A1 (en)
TR (1) TR200202424T2 (en)
WO (1) WO2001081617A1 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2005302085B2 (en) * 2004-11-07 2011-03-17 Cupron Inc. Copper containing materials for treating wounds, burns and other skin conditions
US20100240082A1 (en) 2009-03-18 2010-09-23 Syngenta Participations Ag Methods for detecting and measuring polysaccharide-hydrolyzing enzymes
FI20165270A (en) * 2016-03-31 2017-10-01 Kemira Oyj Method and system for determining starch in a sample
EP3463058B1 (en) 2016-05-31 2023-11-15 Indian Institute of Technology, Guwahati A transmittance based system/kit for point-of-care quantification of biomarkers sample and use thereof

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3888739A (en) * 1973-05-07 1975-06-10 Bio Reagents & Diagnostics Inc Reagents and methods for determining amylase concentrations
JPS6058099A (en) * 1983-09-12 1985-04-04 Konishiroku Photo Ind Co Ltd Element for dry analysis of alpha-amylase

Also Published As

Publication number Publication date
CA2407563A1 (en) 2001-11-01
TR200202424T2 (en) 2003-02-21
AU2001260219A1 (en) 2001-11-07
EP1278888A1 (en) 2003-01-29
JP2003530891A (en) 2003-10-21
WO2001081617A1 (en) 2001-11-01

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Legal Events

Date Code Title Description
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE A 4A, 5A, 6A E 7A ANUIDADES.

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: REFERENTE AO DESPACHO PUBLICADO NA RPI 1963 DE 19.08.2008.