BR0110214A - Yeast Cell and Methods for Detecting the Interaction of a First Peptide with a Second Peptide of a Peptide Binding Pair, Determining the Interaction of a Test Sample with a First or Second Peptides of a Peptide Binding Pair, and simultaneous detection of the interaction of two different peptide binding pairs - Google Patents
Yeast Cell and Methods for Detecting the Interaction of a First Peptide with a Second Peptide of a Peptide Binding Pair, Determining the Interaction of a Test Sample with a First or Second Peptides of a Peptide Binding Pair, and simultaneous detection of the interaction of two different peptide binding pairsInfo
- Publication number
- BR0110214A BR0110214A BR0110214-1A BR0110214A BR0110214A BR 0110214 A BR0110214 A BR 0110214A BR 0110214 A BR0110214 A BR 0110214A BR 0110214 A BR0110214 A BR 0110214A
- Authority
- BR
- Brazil
- Prior art keywords
- peptide
- interaction
- peptide binding
- binding pair
- fusion protein
- Prior art date
Links
- 108090000765 processed proteins & peptides Proteins 0.000 title abstract 12
- 230000027455 binding Effects 0.000 title abstract 6
- 230000003993 interaction Effects 0.000 title abstract 6
- 238000000034 method Methods 0.000 title abstract 3
- 238000001514 detection method Methods 0.000 title abstract 2
- 210000005253 yeast cell Anatomy 0.000 title abstract 2
- 102000004196 processed proteins & peptides Human genes 0.000 title 1
- 108020001507 fusion proteins Proteins 0.000 abstract 5
- 102000037865 fusion proteins Human genes 0.000 abstract 5
- 230000029279 positive regulation of transcription, DNA-dependent Effects 0.000 abstract 5
- 108090000623 proteins and genes Proteins 0.000 abstract 5
- 210000004027 cell Anatomy 0.000 abstract 4
- 102000004169 proteins and genes Human genes 0.000 abstract 3
- 230000004568 DNA-binding Effects 0.000 abstract 2
- 108060001084 Luciferase Proteins 0.000 abstract 1
- 239000013611 chromosomal DNA Substances 0.000 abstract 1
- 238000012217 deletion Methods 0.000 abstract 1
- 230000037430 deletion Effects 0.000 abstract 1
- 238000012216 screening Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/72—Receptors; Cell surface antigens; Cell surface determinants for hormones
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
- C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/61—Growth hormone [GH], i.e. somatotropin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Endocrinology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Gastroenterology & Hepatology (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Toxicology (AREA)
- Mycology (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Immunology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
Abstract
"CéLULA DE LEVEDURA E MéTODOS DE DETECçãO DA INTERAçãO DE UM PRIMEIRO PEPTìDIO COM UM SEGUNDO PEPTìDIO DE UM PAR DE LIGAçãO DE PEPTìDIOS, DE DETERMINAçãO DA INTERAçãO DE UMA AMOSTRA DE TESTE COM UM PRIMEIRO OU UM SEGUNDO PEPTìDIOS DE UM PAR DE LIGAçãO DE PEPTìDIOS E DE DETECçãO SIMULTâNEA DA INTERAçãO DE DOIS PARES DE LIGAçãO DE PEPTìDIOS DIFERENTES". Esta invenção refere-se a novas células hospedeiras modificadas que expressam proteínas fundidas heterólogas e métodos de triagem de amostras de teste com atividade de ligação de peptídios em que a célula hospedeira modificada compreende: (a) uma seq³ência de genes que codifica uma proteína de fusão heteróloga, compreendendo a referida proteína de fusão um primeiro peptídio de um par de ligação de peptídios ou segmento do citado primeiro peptídio, que se junta, quer a um domínio de ligação de DNA, quer ao seu correspondente domínio de ativação transcricional de uma proteína de ativação transcricional; (b) uma seq³ência de genes que codifica uma proteína de fusão heteróloga, compreendendo a referida proteína de fusão um segundo peptídio do par de ligação de peptídios de (a) ou um segmento do mesmo, fundido, quer a um domínio de ligação de DNA, quer ao seu domínio correspondente de ativação transcricional, nenhum dos quais é empregado em (a); (c) um gene luciferase operativamente associado à proteína de ativação transcricional ou parte da mesma; (d) opcionalmente, uma deleção ou mudança no DNA cromossómico da célula hospedeira para a proteína de ativação transcricional, se presente na célula hospedeira selecionada."Yeast Cell and Methods of Detecting the Interaction of a FIRST PEPTIDUS WITH A SECOND PETITUDE CONNECTION, DETERMINING THE INTERACTION OF A TEST SAMPLE WITH A FIRST AND A SECOND PEPTID PETITUS DETECTION PROTECTION OF THE INTERACTION OF TWO DIFFERENT PETIDE CONNECTION PAIRS ". This invention relates to novel modified host cells expressing heterologous fused proteins and peptide binding activity test sample screening methods wherein the modified host cell comprises: (a) a gene sequence encoding a fusion protein said fusion protein, said fusion protein comprising a first peptide of a peptide binding pair or segment of said first peptide, which joins either a DNA binding domain or its corresponding transcriptional activation domain of a transcriptional activation; (b) a gene sequence encoding a heterologous fusion protein, said fusion protein comprising a second peptide of the peptide binding pair of (a) or a segment thereof fused to either a DNA binding domain. either its corresponding transcriptional activation domain, none of which is employed in (a); (c) a luciferase gene operably associated with or part of the transcriptional activation protein; (d) optionally, a deletion or change in chromosomal DNA from the host cell to the transcriptional activation protein, if present in the selected host cell.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/556,390 US6770446B1 (en) | 1994-06-14 | 2000-04-24 | Cell systems having specific interaction of peptide binding pairs |
| PCT/US2001/013006 WO2001081548A1 (en) | 2000-04-24 | 2001-04-23 | Novel cell systems having specific interaction of peptide binding pairs |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR0110214A true BR0110214A (en) | 2003-01-21 |
Family
ID=24221147
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR0110214-1A BR0110214A (en) | 2000-04-24 | 2001-04-23 | Yeast Cell and Methods for Detecting the Interaction of a First Peptide with a Second Peptide of a Peptide Binding Pair, Determining the Interaction of a Test Sample with a First or Second Peptides of a Peptide Binding Pair, and simultaneous detection of the interaction of two different peptide binding pairs |
Country Status (7)
| Country | Link |
|---|---|
| EP (1) | EP1283870A4 (en) |
| JP (1) | JP2004527204A (en) |
| AU (1) | AU2001257157A1 (en) |
| BR (1) | BR0110214A (en) |
| CA (1) | CA2407382A1 (en) |
| MX (1) | MXPA02010446A (en) |
| WO (1) | WO2001081548A1 (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004041193A2 (en) * | 2002-11-01 | 2004-05-21 | Decode Genetics Ehf. | HUMAN TYPE II DIABETES GENE-Kv CHANNEL-INTERACTING PROTEIN (KChIP1) LOCATED ON CHROMOSOME 5 |
| JP4900718B2 (en) * | 2005-06-09 | 2012-03-21 | 独立行政法人産業技術総合研究所 | Reporter assay using secretory luminescent enzyme |
| EP2771461B1 (en) * | 2011-10-28 | 2019-12-04 | Merck Sharp & Dohme Corp. | Engineered lower eukaryotic host strains for recombinant protein expression |
| GB201520021D0 (en) * | 2015-11-13 | 2015-12-30 | Asterion Ltd | Fusion polypeptide |
| CN113234748B (en) * | 2021-05-11 | 2022-11-08 | 中国海洋大学 | RXR yeast transcription activation system and labeled sediment toxicology experiment method thereof |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5283173A (en) * | 1990-01-24 | 1994-02-01 | The Research Foundation Of State University Of New York | System to detect protein-protein interactions |
| US5283179A (en) * | 1990-09-10 | 1994-02-01 | Promega Corporation | Luciferase assay method |
| US5622852A (en) * | 1994-10-31 | 1997-04-22 | Washington University | Bcl-x/Bcl-2 associated cell death regulator |
| US5837478A (en) * | 1993-12-23 | 1998-11-17 | Icos Corporation | Method of identifying modulators of binding between and VCAM-1 |
| US5525490A (en) * | 1994-03-29 | 1996-06-11 | Onyx Pharmaceuticals, Inc. | Reverse two-hybrid method |
| US5989808A (en) * | 1994-06-14 | 1999-11-23 | American Cyanamid Company | Identification of compounds affecting specific interaction of peptide binding pairs |
-
2001
- 2001-04-23 EP EP01930643A patent/EP1283870A4/en not_active Withdrawn
- 2001-04-23 JP JP2001578619A patent/JP2004527204A/en active Pending
- 2001-04-23 MX MXPA02010446A patent/MXPA02010446A/en unknown
- 2001-04-23 WO PCT/US2001/013006 patent/WO2001081548A1/en not_active Application Discontinuation
- 2001-04-23 CA CA002407382A patent/CA2407382A1/en not_active Abandoned
- 2001-04-23 AU AU2001257157A patent/AU2001257157A1/en not_active Abandoned
- 2001-04-23 BR BR0110214-1A patent/BR0110214A/en not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| EP1283870A4 (en) | 2004-05-12 |
| JP2004527204A (en) | 2004-09-09 |
| AU2001257157A1 (en) | 2001-11-07 |
| MXPA02010446A (en) | 2003-04-25 |
| EP1283870A1 (en) | 2003-02-19 |
| WO2001081548A1 (en) | 2001-11-01 |
| CA2407382A1 (en) | 2001-11-01 |
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