BR0013896A - Nucleotide sequence, vector, transformed filamentous fungi, method of producing a protein of interest, protein, and, expression system to produce a protein of interest - Google Patents

Nucleotide sequence, vector, transformed filamentous fungi, method of producing a protein of interest, protein, and, expression system to produce a protein of interest

Info

Publication number
BR0013896A
BR0013896A BR0013896-7A BR0013896A BR0013896A BR 0013896 A BR0013896 A BR 0013896A BR 0013896 A BR0013896 A BR 0013896A BR 0013896 A BR0013896 A BR 0013896A
Authority
BR
Brazil
Prior art keywords
protein
interest
producing
vector
produce
Prior art date
Application number
BR0013896-7A
Other languages
Portuguese (pt)
Inventor
Theresa White
Sylvia Mchugh
Christopher D Hindle
Original Assignee
Iogen Bio Products Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/392,476 external-priority patent/US6939704B1/en
Application filed by Iogen Bio Products Corp filed Critical Iogen Bio Products Corp
Publication of BR0013896A publication Critical patent/BR0013896A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0055—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10)
    • C12N9/0057—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10) with oxygen as acceptor (1.10.3)
    • C12N9/0061—Laccase (1.10.3.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2445—Beta-glucosidase (3.2.1.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01021—Beta-glucosidase (3.2.1.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

"SEQuêNCIA DE NUCLEOTìDEO, VETOR, FUNGOS FILAMENTOSOS TRANSFORMADOS, MéTODO DE PRODUZIR UMA PROTEìNA DE INTERESSE, PROTEìNA, E, SISTEMA DE EXPRESSãO PARA PRODUZIR UMA PROTEìNA DE INTERESSE". A presente invenção refere-se ao aumento da produção de uma proteina de interesse de um hospedeiro fúngico. A invenção descreve seq³ências de nucleotídeo compreendendo uma região reguladora em associação operativa com uma seq³ência de secreção de xilanase e um gene de interesse. O gene de interesse codifica uma proteina selecionada de farmacêutico, nutracêutico, industrial, alimentação animal, aditivo alimenticio ou uma enzima. Preferivelmente, o gene de interesse codifica uma celulase, hemicelulase, uma enzima de degradação da lignina, pectinase, protease ou peroxidase. A presente invenção também refere-se a vetores e hospedeiros compreendendo estas seq³ências de ácido nucleico e a métodos para a produção de uma proteina de interesse."NUCLEOTIDE SEQUENCE, VECTOR, TRANSFORMED FILMARY FUNGI, METHOD OF PRODUCING A PROTEIN OF INTEREST, PROTEIN, AND, EXPRESSION SYSTEM TO PRODUCE A PROTEIN OF INTEREST". The present invention relates to increasing the production of a protein of interest to a fungal host. The invention describes nucleotide sequences comprising a regulatory region in operative association with a xylanase secretion sequence and a gene of interest. The gene of interest encodes a protein selected from pharmaceutical, nutraceutical, industrial, animal feed, food additive or an enzyme. Preferably, the gene of interest encodes a cellulase, hemicellulase, a lignin-degrading enzyme, pectinase, protease or peroxidase. The present invention also relates to vectors and hosts comprising these nucleic acid sequences and methods for producing a protein of interest.

BR0013896-7A 1999-09-09 2000-09-01 Nucleotide sequence, vector, transformed filamentous fungi, method of producing a protein of interest, protein, and, expression system to produce a protein of interest BR0013896A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/392,476 US6939704B1 (en) 1998-03-10 1999-09-09 Enhanced expression of proteins in genetically modified fungi
PCT/CA2000/000997 WO2001018218A1 (en) 1999-09-09 2000-09-01 Expression of proteins in genetically modified fungi

Publications (1)

Publication Number Publication Date
BR0013896A true BR0013896A (en) 2002-05-14

Family

ID=23550748

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0013896-7A BR0013896A (en) 1999-09-09 2000-09-01 Nucleotide sequence, vector, transformed filamentous fungi, method of producing a protein of interest, protein, and, expression system to produce a protein of interest

Country Status (7)

Country Link
EP (1) EP1214431A1 (en)
CN (1) CN1390260A (en)
AU (1) AU6974400A (en)
BR (1) BR0013896A (en)
CA (1) CA2382609C (en)
MX (1) MX250271B (en)
WO (1) WO2001018218A1 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK1516053T3 (en) 2002-06-14 2013-03-25 Verenium Corp Xylanases, their coding nucleic acids, and processes for their preparation and use
WO2007095398A2 (en) 2006-02-14 2007-08-23 Verenium Corporation Xylanases, nucleic acids encoding them and methods for making and using them
US8198046B2 (en) 2006-07-11 2012-06-12 Danisco Us Inc. KEX2 cleavage regions of recombinant fusion proteins
US20080026376A1 (en) * 2006-07-11 2008-01-31 Huaming Wang KEX2 cleavage regions of recombinant fusion proteins
US8518684B2 (en) 2008-11-18 2013-08-27 Novozymes, Inc. Methods and compositions for degrading cellulosic material
CN111690629B (en) * 2020-05-29 2022-04-19 浙江工业大学 Endoglucanase mutant, gene, engineering bacterium and application thereof
CN114426987B (en) * 2022-01-14 2024-05-24 湖南省蔬菜研究所 Genetic transformation method for capsicum southern blight germ

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993000426A1 (en) * 1991-06-25 1993-01-07 Novo Nordisk A/S Mammalian pancreatic lipase and variant thereof
US5591619A (en) * 1994-09-30 1997-01-07 University Of Georgia Research Foundation, Inc. Aureobasidium pullulans xylanase, gene and signal sequence
US6015703A (en) * 1998-03-10 2000-01-18 Iogen Corporation Genetic constructs and genetically modified microbes for enhanced production of beta-glucosidase

Also Published As

Publication number Publication date
WO2001018218A9 (en) 2002-11-07
EP1214431A1 (en) 2002-06-19
MX250271B (en) 2007-10-12
WO2001018218B1 (en) 2001-04-19
CN1390260A (en) 2003-01-08
MXPA02002516A (en) 2005-11-17
WO2001018218A1 (en) 2001-03-15
CA2382609C (en) 2012-08-07
CA2382609A1 (en) 2001-03-15
AU6974400A (en) 2001-04-10

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Legal Events

Date Code Title Description
B11A Dismissal acc. art.33 of ipl - examination not requested within 36 months of filing
B11Y Definitive dismissal - extension of time limit for request of examination expired [chapter 11.1.1 patent gazette]