BR0012873A - Nucleic acid sequences encoding polyenoic fatty acid isomerase and uses thereof - Google Patents

Nucleic acid sequences encoding polyenoic fatty acid isomerase and uses thereof

Info

Publication number
BR0012873A
BR0012873A BR0012873-2A BR0012873A BR0012873A BR 0012873 A BR0012873 A BR 0012873A BR 0012873 A BR0012873 A BR 0012873A BR 0012873 A BR0012873 A BR 0012873A
Authority
BR
Brazil
Prior art keywords
nucleic acid
acid sequences
isomerase
fatty acid
sequences encoding
Prior art date
Application number
BR0012873-2A
Other languages
Portuguese (pt)
Inventor
Wei Zheng
Ling Yuan
James George Metz
Original Assignee
Monsanto Technology Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Monsanto Technology Llc filed Critical Monsanto Technology Llc
Publication of BR0012873A publication Critical patent/BR0012873A/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

"SEQuêNCIAS DE áCIDO NUCLéICO QUE CODIFICAM ISOMERASE DE áCIDO GRAXO POLIENóICO E USOS DAS MESMAS". São proporcionados métodos e sequências de ácido nucleico para a produção de ácidos graxos conjugados em células hospedeiras. As seq³ências de ácido nucleico são obtidas a partir de fontes algais as quais codificam a isomerase de ácido graxo polienóico. As seq³ências de ácido nucleico podem ser usadas em estruturas de expressão para dirigir a expressão das seq³ências de PFI em células hospedeiras. A expressão da PFI em células hospedeiras transgênicas proporcionar a produção de ácidos graxos conjugados na célula hospedeira."NUCLEIC ACID SEQUENCES THAT CODE POLYENOIC FATTY ACID ISOMERASE AND USES OF THE SAME". Nucleic acid methods and sequences for the production of conjugated fatty acids in host cells are provided. The nucleic acid sequences are obtained from algal sources which encode the polyenoic fatty acid isomerase. Nucleic acid sequences can be used in expression structures to direct the expression of PFI sequences in host cells. The expression of PFI in transgenic host cells provide the production of conjugated fatty acids in the host cell.

BR0012873-2A 1999-07-30 2000-07-28 Nucleic acid sequences encoding polyenoic fatty acid isomerase and uses thereof BR0012873A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US14645899P 1999-07-30 1999-07-30
PCT/US2000/020597 WO2001009296A1 (en) 1999-07-30 2000-07-28 Nucleic acid sequences encoding polyenoic fatty acid isomerase and uses thereof

Publications (1)

Publication Number Publication Date
BR0012873A true BR0012873A (en) 2002-04-16

Family

ID=22517454

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0012873-2A BR0012873A (en) 1999-07-30 2000-07-28 Nucleic acid sequences encoding polyenoic fatty acid isomerase and uses thereof

Country Status (6)

Country Link
EP (1) EP1218497A1 (en)
AU (1) AU6749400A (en)
BR (1) BR0012873A (en)
CA (1) CA2379813A1 (en)
MX (1) MXPA02001086A (en)
WO (1) WO2001009296A1 (en)

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5856149A (en) * 1995-06-01 1999-01-05 Wisconsin Alumni Research Foundation Method of producing conjugated fatty acids
JP2002508929A (en) * 1997-12-23 2002-03-26 ディーシーブイ・インコーポレイテッド・ドゥーイング・ビジネス・アズ・バイオ−テクニカル・リソーシィズ Linoleate isomerase
JPH11253162A (en) * 1998-03-10 1999-09-21 Mitsubishi Chemical Corp Dna encoding monoamine oxidase

Also Published As

Publication number Publication date
EP1218497A1 (en) 2002-07-03
WO2001009296A1 (en) 2001-02-08
AU6749400A (en) 2001-02-19
MXPA02001086A (en) 2002-08-12
CA2379813A1 (en) 2001-02-08

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Legal Events

Date Code Title Description
FA10 Dismissal: dismissal - article 33 of industrial property law
B11Y Definitive dismissal - extension of time limit for request of examination expired [chapter 11.1.1 patent gazette]