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The method is used in the production of insulin preparations. This method reduces the high percentage of non-specific binding in radioimmunological assays of porcine proinsulin in insulin preparations, since the proposed method provides increased purity of such preparations. Implementation of the method involves incubation of the substance to be tested with a buffer system and antiserum for four hours, addition of <125>I-tagged proinsulin, and continued incubation for an additional 16 hours, followed by separation of the non-radioactively-tagged material and measurement of the radioactivity.
BG9546391A1991-11-131991-11-13Method for radioimmunological determination of swine proinsulin
BG51657A1
(en)