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Priority to BG9048989ApriorityCriticalpatent/BG49634A1/en
Publication of BG49634A1publicationCriticalpatent/BG49634A1/en
The hormone is applied in biotechnology and medicine for diagnostic and experimental purposes. The method is easily implementable and it derives a hormone of relatively high output - from 20 to 30 mkg/g of tissue, with specific activity of 11.2 ME/mg of protein and enhanced summating hormonal activity calculated on the basis of weight of hypophisic tissue. It consists of extraction of frozen and homogeneized human hypophisic glands with a buffer, centrifusion and subsequent precipitation with ammonium sulphate. The extraction is carried out with a buffer at pH from 8.4 to 8.6, while the homogen is centrifuged at low revolutions. The supernatante is subject to ionic exchange chromatography, the protein is eluated and the derived eluate is precipitated with ammonium sulphate and is centrifuged at low revolutions, after which the precipitation is consecutively solved, desalinated and concentrated via ultra-filtration. The concentrate is subject to ionic-exchange chromatography, then eluation and the eluate is treated through adsorption chromatography. The hormonal product is eluated. The derived eluate is concentrated through ultra-filtration and the concentrate is cleansed through gel-filtration.
BG9048989A1989-11-291989-11-29Method for prepraing of thioreotropen hormone
BG49634A1
(en)
Human derived monocyte attracting purified protein product useful in a method of treating infection and neoplasm in human body, and the cloning of full length cdna thereof
PEPTIDES COMPRISING THE SERYL-ASPARTYL-LYSYL-PROLYLE SEQUENCE, PROCESS FOR THE EXTRACTION OF THE CORRESPONDING TETRAPEPTIDE, AND APPLICATIONS, IN PARTICULAR FOR THE PROTECTION OF THE BONE MARROW DURING CHEMOTHERAPY ANTI-CANCER TREATMENTS
Biologically MEANS THAT HAS immunomodulating property, the way it is received, preparations based on it NORMALIZATION AND METHOD physiological state of the human body and animals in diseases generated MAINLY functional insufficiency of organs and tissues, using such preparation