BG48747A1 - Method for unsulating and cleaning of beta- galactosidase - Google Patents
Method for unsulating and cleaning of beta- galactosidaseInfo
- Publication number
- BG48747A1 BG48747A1 BG8723689A BG8723689A BG48747A1 BG 48747 A1 BG48747 A1 BG 48747A1 BG 8723689 A BG8723689 A BG 8723689A BG 8723689 A BG8723689 A BG 8723689A BG 48747 A1 BG48747 A1 BG 48747A1
- Authority
- BG
- Bulgaria
- Prior art keywords
- mpa
- pressure
- filtration
- cleaning
- pore size
- Prior art date
Links
Landscapes
- Separation Using Semi-Permeable Membranes (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
This method is intended for use in the biotechnology industry. Using this method the enzyme is rapidly and effectively isolated from the biological bulk of Kluyveromyces Lactis and is cleaned without loss of its natural properties. This method is based on separation of the Kl. lactis cells from the fermenting liquid by micro filtration through membranes of 2.00 micrometer pore size at a pressure of 0.1 MPa. The cells are then suspended in a potassium/phosphate buffer of up to 5% dry weight and are destroyed by a homogenizing device, as a result of the high pressure of the flat head with a ballpoint operating at 65 MPa. The flow rate is 23.3 dm<3>/h, the temperature of the flow is 285 degrees K, which facilitates separation of the enzyme solution from the cell remains by filtration through a 0.4 micrometer pore size membrane under a pressure of 0.12 MPa. The substance is then subjected to cleaning and concentration using ultra filtration at a pressure of 1 MPa through 200 000 permeability limit membranes.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
BG8723689A BG48747A1 (en) | 1989-02-14 | 1989-02-14 | Method for unsulating and cleaning of beta- galactosidase |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
BG8723689A BG48747A1 (en) | 1989-02-14 | 1989-02-14 | Method for unsulating and cleaning of beta- galactosidase |
Publications (1)
Publication Number | Publication Date |
---|---|
BG48747A1 true BG48747A1 (en) | 1991-05-15 |
Family
ID=3921546
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BG8723689A BG48747A1 (en) | 1989-02-14 | 1989-02-14 | Method for unsulating and cleaning of beta- galactosidase |
Country Status (1)
Country | Link |
---|---|
BG (1) | BG48747A1 (en) |
-
1989
- 1989-02-14 BG BG8723689A patent/BG48747A1/en unknown
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