The United States Of America, As Represented By The Secretary Of Agriculture
Andrew ALPERT
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Priority claimed from US09/420,850external-prioritypatent/US6150172A/en
Application filed by The United States Of America, As Represented By The Secretary Of Agriculture, Andrew ALPERTfiledCriticalThe United States Of America, As Represented By The Secretary Of Agriculture
Publication of BG105791ApublicationCriticalpatent/BG105791A/en
Publication of BG64291B1publicationCriticalpatent/BG64291B1/en
A method for extracting prion protein from a biological material, e.g., an animal tissue or product. In a specific example, abnormal prion protein is extracted from homogenized sheep brain with hexafluoro-2-propanol. The hexafluoto-2-propanol is separated from the aqueous brain preparation by increasing the ionic strength of the aqueous solution. Prion protein in the organic extract can be further purified, or the extract can be tested, e.g., by immunoassay, for the presence of prion protein, and more particularly abnormal prion protein. The extraction process permits testing for the presence of abnormal prion protein, e.g., for diagnoses or transmissible spongioform encephalopathies (TSE). 26 claims, 2 figures
BG105791A1999-01-082001-08-07Method and kit for extracting prion protein
BG64291B1
(en)
Light and electron microscopic immunocytochemical localization of parvalbumin in the dorsal lateral geniculate nucleus of the cat: evidence for coexistence with GABA
Characterization of the Translocation of Protein Kinase C (PKC) by 3, 4-methylenedioxymethamphetamine (MDMA/Ecstasy) in Synaptosomes:: Evidence for a Presynaptic Localization Involving the Serotonin Transporter (SERT)