BE792290A - Orgatein isolation - using protidolytic enzymes, thermic degradation and chromatography - Google Patents
Orgatein isolation - using protidolytic enzymes, thermic degradation and chromatographyInfo
- Publication number
- BE792290A BE792290A BE792290DA BE792290A BE 792290 A BE792290 A BE 792290A BE 792290D A BE792290D A BE 792290DA BE 792290 A BE792290 A BE 792290A
- Authority
- BE
- Belgium
- Prior art keywords
- orgotein
- proteins
- protidolytic
- haemoglobin
- eluate
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0089—Oxidoreductases (1.) acting on superoxide as acceptor (1.15)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Peptides Or Proteins (AREA)
Abstract
Orgateins are isolated from mixtures of soluble proteins using >=1 of the following purification stages (1) an aq. soln of the protein mixture is treated with a protidolytic enzyme which selectively degrades one fraction of the proteins other than orgotein (2) at least the erythrocyte fraction of whole blood is heated at 60-80 degrees C and pH 5-8 to precipitate haemoglobin, the precipitate is separated and orgotein isolated from supernatant liquid (3). A mixture of orgotein and other proteins is dissolved in an aqs. buffer at pH 6 to give a soln of ionic strength 0-01 M and chromatographed on a weakly basic ion exchange resin (e.g. DEAE-cellulose TEAE-cellulose or cross-linked dextran having diethylaminoethyl gps). The resin is selectively eluted with an eluent having greater ionic strength (pref. increased progressively from 0.02 M to >0.03 M) at pH 6 with simultaneous determination of the absorption at 2650 and/or 2800 angstroms and the Cu and/or Zn content of the eluate and separation of the eluate which has a maximum non-ionised metal content and a minimum absorption at 265 or 280 nm. When the soluble protein mixture is obtained from erythrocytes the haemoglobin and carbonic-anhydrase are removed by washing the column prior to the elution of the orgotein.
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US20560971A | 1971-12-07 | 1971-12-07 | |
| US20561071A | 1971-12-07 | 1971-12-07 | |
| US27327772A | 1972-07-19 | 1972-07-19 | |
| US00273278A US3806411A (en) | 1972-07-19 | 1972-07-19 | Enzymatic treatment of protein mixtures containing orgotein |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BE792290A true BE792290A (en) | 1973-03-30 |
Family
ID=27498583
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BE792290D BE792290A (en) | 1971-12-07 | 1972-12-05 | Orgatein isolation - using protidolytic enzymes, thermic degradation and chromatography |
Country Status (2)
| Country | Link |
|---|---|
| AR (1) | AR194149A1 (en) |
| BE (1) | BE792290A (en) |
-
1972
- 1972-12-05 BE BE792290D patent/BE792290A/en unknown
- 1972-12-07 AR AR24554772A patent/AR194149A1/en active
Also Published As
| Publication number | Publication date |
|---|---|
| AR194149A1 (en) | 1973-06-22 |
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