AU7940194A - Method for culturing bipolar-adhesion hepatocytes - Google Patents

Method for culturing bipolar-adhesion hepatocytes

Info

Publication number
AU7940194A
AU7940194A AU79401/94A AU7940194A AU7940194A AU 7940194 A AU7940194 A AU 7940194A AU 79401/94 A AU79401/94 A AU 79401/94A AU 7940194 A AU7940194 A AU 7940194A AU 7940194 A AU7940194 A AU 7940194A
Authority
AU
Australia
Prior art keywords
matrix
serum
free culture
hepatocytes
bipolar
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU79401/94A
Inventor
Augustinus Bader
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of AU7940194A publication Critical patent/AU7940194A/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • C12N5/0671—Three-dimensional culture, tissue culture or organ culture; Encapsulated cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

A method of adding extracellular matrix components to the stationary phase of the matrix for bipolar adhesion of hepatocytes enables these substances to be concentrated and bonded to a defined base matrix composed of a simple base matrix (e.g. type I collagen). One advantage, for example when fibronectin is added, is to provide a completely serum-free culture of liver cells while maintaining normal morphology and function; as a result conventional culture media previously unsuited to serum-free culture of liver cells are now also suitable for relatively long-term serum-free culture. Furthermore, reproducibility of trial results and mechanical stability are substantially improved over the conventional form of matrix layering. The method is applied using a specially adapted kit. Other matrix components can also be added to the matrix by this method, and thus concentration and bonding can be effected within the fibrillary matrix skeleton after the base matrix has gelled.
AU79401/94A 1993-10-26 1994-10-25 Method for culturing bipolar-adhesion hepatocytes Abandoned AU7940194A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE4336399 1993-10-26
DE4336399A DE4336399A1 (en) 1993-10-26 1993-10-26 Process for improving the matrix conditions of bipolar adhered hepatocytes and for producing a suitably configured cell culture kit
PCT/EP1994/003501 WO1995011963A1 (en) 1993-10-26 1994-10-25 Method for culturing bipolar-adhesion hepatocytes

Publications (1)

Publication Number Publication Date
AU7940194A true AU7940194A (en) 1995-05-22

Family

ID=6500981

Family Applications (1)

Application Number Title Priority Date Filing Date
AU79401/94A Abandoned AU7940194A (en) 1993-10-26 1994-10-25 Method for culturing bipolar-adhesion hepatocytes

Country Status (11)

Country Link
EP (1) EP0725816B1 (en)
JP (1) JP2004537951A (en)
AT (1) ATE190648T1 (en)
AU (1) AU7940194A (en)
CA (1) CA2174925A1 (en)
DE (2) DE4336399A1 (en)
DK (1) DK0725816T3 (en)
ES (1) ES2145845T3 (en)
NO (1) NO961653L (en)
PT (1) PT725816E (en)
WO (1) WO1995011963A1 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996001426A1 (en) * 1994-07-01 1996-01-18 W.R. Grace & Co.-Conn. Hepatocyte test kit
CN100510060C (en) 2001-08-15 2009-07-08 宝生物工程株式会社 Method of extended culture for antigen-specific cytotoxic t lymphocytes
ATE491021T1 (en) 2002-03-25 2010-12-15 Takara Bio Inc METHOD FOR PRODUCING A CYTOTOXIC LYMPHOCYTE
DE602004025591D1 (en) * 2003-08-22 2010-04-01 Takara Bio Inc METHOD FOR PRODUCING CYTOTOXIC LYMPHOCYTES
CN101243187B (en) 2005-08-17 2012-07-11 宝生物工程株式会社 Method of producing lymphocytes
JP6454172B2 (en) * 2015-02-12 2019-01-16 日本バイリーン株式会社 Hepatocyte culture method

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4206585C2 (en) * 1992-03-03 1994-11-24 Augustinus Dr Med Bader Device for mass culture of cells
DE4222345A1 (en) * 1992-07-08 1994-01-13 Augustinus Dr Med Bader Process for growing a cell type in a co-culture process with liver cells

Also Published As

Publication number Publication date
JP2004537951A (en) 2004-12-24
ES2145845T3 (en) 2000-07-16
DK0725816T3 (en) 2000-07-31
CA2174925A1 (en) 1995-05-04
NO961653D0 (en) 1996-04-25
DE59409216D1 (en) 2000-04-20
NO961653L (en) 1996-04-25
EP0725816B1 (en) 2000-03-15
ATE190648T1 (en) 2000-04-15
DE4336399A1 (en) 1995-04-27
WO1995011963A1 (en) 1995-05-04
PT725816E (en) 2000-08-31
EP0725816A1 (en) 1996-08-14

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