AU782952B2 - Therapeutic immune condition compositions and methods - Google Patents

Description

WO 01/91792 PCT/IB00/02077 THERAPEUTIC IMMUNE CONDITION COMPOSITIONS AND METHODS CROSS-REFERENCE TO RELATED APPLICATIONS This application claims benefit from U.S. provisional application serial no.

60/150,652 filed August 25, 1999, the entire contents of which are hereby incorporated by reference.

BACKGROUND OF THE INVENTION Field of the Invention This invention relates to compositions for the treatment and/or prevention of immune conditions, particularly inflammation and cancer, and methods for making and using such compositions.

Summary of the Related Art Two immune conditions that affect the health of humans and domesticated animals are inflammation and cancer. Gallin et al. (Inflammation: Basic Principles and Clinical Correlates, Raven Press, New York, 1988) teaches that inflammation is a complex protective physiologic response elicited by various stimuli such as infectious agent, localized tissue injury, or other trauma. The inflammatory response involves numerous mediators and various immune cells. Issekutz et aL (Imununology 88: 569-576, 1996) teaches that the migration of white blood cells leukocytes) from the blood stream through the endothelial cell wall to sites of injury, infection, or immunological reaction is recognized as a characteristic feature and a critical step of the inflammatory response. The accumulation of leukocytes into inflamed tissues occurs as a consequence of endothelial cell activation and leukocyte migration to the inflammatory foci.

Diaz-GonzAlez and Sanchez-Madrid (Inununology Today 19(4): 1-3, 1998) teach that the process of leukocyte migration requires an interaction between leukocytes and the endothelial cells lining the blood vessel. Leukocyte-endothelial cell interaction is a multistep process, which involves a cascade of sequential cellular adhesive events that can be divided into four successive steps as illustrated in Figure 1. The initial contact between WO 01/91792 PCT/IB00/02077 leukocytes and the blood vessel wall is a reversible process that involves rolling of leukocytes on the activated endothelium (step one). This rolling of the leukocytes on endothelial cells is facilitated by the adhesion of a selectin molecule L-selectin) on the leukocyte to its ligand sialyl Lewis X or Sialyl Lewis A) on the endothelial cell. Rolling of leukocytes is followed by activation of the leukocytes by chemokines and cytokines (step two), and firm adhesion of activated leukocytes to the endothelium (step three). Crockett-Torabi and Fantone (Immuno Res. 14: 237-251, 1995) teach that in the last step, leukocytes extravasate into the surrounding tissues by squeezing themselves between endothelial cell junctions and moving toward the inflammatory foci The majority of inflammatory disorders are frequently chronic in duration, and of particular importance are the autoimmune diseases. Autoimmune disease is a group of chronic diseases, in which the immune system not only fail to perform its regular function in protecting the host from invading agents, but also reacts particularly against some of the host autoantigens. This autoimmune response is initiated by cellular, humoral, or both mechanisms, and leads to disease with local inflammation and tissue damage. Multiple sclerosis systemic lupus erythematosus (SLE), Crohns disease, and rheumatoid arthritis (RA) are just few examples of autoimmune diseases.

Pitzalis et aL (Annals of Rheumatic Diseases 53: 287-288, 1994) discuss the role of adhesion molecules in the pathogenesis of RA and other autoimmune diseases. Leukocyte infiltration into tissues is believed to be a major component of the pathologic process leading to joint injury in chronic arthritis, including rheumatoid arthritis. Therefore, adhesion molecules represent another attractive immunotherapeutic target for intervention in RA and other autoimmune diseases.

Selectins and their carbohydrate ligands have been considered very important targets in inflammatory conditions because of their important role in the initial contact between leukocytes and the vascular endothelium at sites of inflammation. McMurray, R.W.

(Seminars in Arthritis Rheumatism 25: 215-234, 1996) teaches that selectins are carbohydrate binding adhesion molecules that share a common structural component consisting of a Ca dependent N-terminal lectin binding domain. McEver, R.P. (Curr Opin Immunol 6: 75-84, 1994) teaches that selectins recognize and bind to specific carbohydrate structures on ligands that are composed of sialic acid, fucose, galactose, mannose, and/or an anionic sulfate or phosphate ester moieties such as sialyl Lewis X sialyl Lewis A and related structures.

WO 01/91792 PCT/IB00/02077 Munro et al. (American Journal of Pathology 141(6): 1397-1405, 1992) teach that the carbohydrate ligand, SLe(x)) [NeuAca2-3Galbl-4(Fucal-3) GlcNAc-] is expressed on the cell surface of monocytes, a polymorphonuclear leukocyte (PMNL), and on approximately or peripheral blood T lymphocytes. SLe(x) is recognized by all selectin adhesion molecules. The SLe(x) isomeric epitope, SLe(a) [NeuAca2-3Galbl-3(Fucal-4) GlcNAc-] (Zhang et al., Tumor Biology 18: 175-187, 1997), is recognized and bound to by E and Pselectins, and possibly L-selectin (Paavonen and Renkonen, American Journal of Pathology 141(6): 1259-1261, 1992). Takada et aL (BiochemBiophys Res Commun 179: 713-19, 1991) teach that SLe(a) is absent on most peripheral blood cells.

Blocking selectins or their ligands with antibodies or oligosaccharides has been attempted in various animal models of inflammation. For example, Weiser et al. (Shock 5(6): 402-407, 1996) describe that in rats that had undergone hour of hindlimb tourniquet ischemia, the administration of anti-P-selectin monoclonal antibody (mAb), PB1.3, allegedly reduced hindlimb injury, but not leukosequestration. Yamada et al. (European Journal of Pharmacology 346: 222-224, 1998) describe that the same anti-P-selectin mAb PB1.3 allegedly reduced infarct size by approximately 38% (2 mg/kg) and 28% (5 mg/kg) in rabbits undergoing 30 minutes of ischemia followed by 5 hour reperfusion. Tojo et al.

(Glycobiology 463-9, 1996) allege that the anti-rat-P-selectin mAb, ARP2-4, attenuated infarct size in rat myocardial ischemia and reperfusion injury model Ohnishi et al.

(Inununopharmacology 34: 161-170, 1996) describe that the anti-rat-P-selectin mAb, ARP2- 4, allegedly reduced the footpad swelling and inhibited the associated polymorphonuclear leukocyte (PMNL) accumulation when administered to rats who had undergone dermal injury induced by Arthus reaction.

Altavilla et al. (European Journal of Pharmacology 270: 45-51,1994) allege that the use of the anti-E-selectin mAb, BBIG-E5, resulted in the reduction in infarct size as well as PMNL accumulation in rodent model.

A study on endotoxin-induced uveitis in mice (Whitcup et al., Clinical Inumuology and Iununopathology 83(1): 45-52, 1997) reported that neutrophil migration into the eye was allegedly effectively inhibited with a combination of anti- E-selectin and anti-P-selectin monoclonal antibodies administered intraperitoneally, either before or after endotoxin injection. In this study, anti-E-selectin antibody alone allegedly had little effect on endotoxin-induced uveitis, while anti-P-selectin mAb decreased ocular inflammation by 37% when administered at the time of or 6 hours after endotoxin injection.

WO 01/91792 PCT/IB00/02077 Ma et al. (Circulation 88: 649-658, 1993) allege that a reduction in infarct size as well as PMNL accumulation was observed with the use anti-L-selectin mAb, DREG-200, in a feline model. Pizcueta and Luscinskas (American Journal of Pathology 145(2): 461-467, 1994) disclose an in vivo murine model of experimentally induced chronic inflammation of the peritoneum in which anti-L-selectin mAb (MEL-14) was infused intravenously to investigate the role of L-selectin in the recruitment of mononuclear leukocytes to chronic sites of inflammation (48 hours). Results from this study showed an allegedly reduced accumulation of macrophages and lymphocytes by 60% and 90%, respectively, at 48 hours.

Similarly, MEL-14 mAb allegedly dramatically inhibited granulocyte influx by 80% at 6 hours and by 50% at 24 and 48 hours.

Most of the studies on selectin-carbohydrate ligands have targeted Sialyl Lewis X oligosaccharide molecule. Tojo et al. (Glycobiology 463-9, 1996) describe the effect of SLe(x)-oligosaccharide on rat myocardial ischemia and reperfusion injury. In this study, the administration of SLe(x)-oligosaccharide allegedly caused significant reduction in infarct size without affecting hemodynamic parameters or circulating leukocyte numbers.

Han et al. (Journal of Immunology 155(8): 4011-5, 1995) describe the effect of a soluble SLe(x) on ear edema and necrosis in rabbits where the ear vascular supply was occluded for 6 hours, and allowed to reperfuse later. In this study, tissue edema and necrosis were allegedly significantly reduced in animals treated with SLe(x) (25 mg kg bolus i.v.

followed by 50 mg/kg infusion over 10 hour) immediately upon reperfusion or after a 1-hour delay, but not in animals for whom the treatment was delayed 4 or 12 hours.

Schmid et al. (Journal of Heart Lung Transplantation 16(10): 1054-1061, 1997) evaluated the inhibitory effect of CY-1503, an analogue of SLe(x), on PMNL migration and reperfusion injury in canine left lung allografts (35 mg/kg i.v. bolus) where the recipient contralateral right pulmonary artery and bronchus were ligated. In this study, allograft gas exchange and hemodynamics results allegedly demonstrated that the group treated with CY- 1503 had a reduction in PMNL adhesion, migration, and subsequent reperfusion injury in preserved canine lung allografts. Birnbaum et al. (Journal of Molecular Cellular Cardiology 29(8): 2013-25, 1997) discloses that the treatment with CY-1503 (30 mg/kg i.v.

bolus) allegedly did not limit infarct size or prevent the "no-reflow" phenomenon in rabbits who were subjected to 30 minutes of coronary artery occlusion and 4 hour of reperfusion.

Yet additional studies demonstrated that SLe(x) oligosaccharide allegedly reduced infarct 28. Jul. 2005 10:34 Shelston IP No. 7515 P. 4 size by 83% and inhibited PMNL adhesion to endothelial cells in the feline model (Buerke et al., Journal of Clinical Investigation 93: 1140-1148,1994); and in the canine model it allegedly reduced infarct size by 67% and ameliorated PMNL accumulation in myocardium by 63% (Lefer et al., Circulation 90: 2390-2401,1994).

In addition, the effect of anti-SLe(x) mAb and the expression of SLe(x) were examined in rat hearts subjected to 30 minutes of ischemia followed by reperfusion (Yoshinori et al.. Journal ofPathology 180: 305-310,1996). Reperfusion of ischaemic myocardial tissue in this study allegedly resulted in enhanced expression of SLe(x) on the luminal surface of vascular endothelial cells, as well as myocytes. Furthermore, the in vive administration of anti-SLe(x) mAb significantly reduced the extent of the myocardial infarction.

The mortality from cancer often arises not from the primary tumor site, but from the secondary tumor sites caused by migration of metastatic cancer cells. Many cancer cells, like many blood cells, express sialyl Lewis X and/or sialyl Lewis A (Ravindranath et AL., Cancer 79(9): 1686-1697, 1997 Tomlinson et al., Int. J. Oncol. 16(2):347-353, 2000; Skorstengaard et al, J. Urol. 161(4):1316-1323, 1999 Groves et al., Am. J.

Pathol 143(4): 1220-1225, 1993; Farmer et al., Head Neck 20(8):726-731,1998).

Fukuda et al.. Cancer Res. 60(2):450-456, 2000) allege that a peptide mimic ofE- Sselectin ligand inhibits the lung colonization of melanoma cells expressing sialyl Lewis *20 X.

Although numerous studies have attempted to use antibodies against adhesion 1 molecules to reduce immune conditions in animal models, because such high dosages of antibody are required, these approaches may result in antibody-dependent cellular cytotoxicity (ADCC) or antibody-mediated toxicity in human patients. Thus, there remains a need to develop safer approaches to reducing immune conditions that allow for the administration of lower dosages of binding agents, such as antibodies, to adhesion molecules.

Any discussion of the prior art throughout the specification should in no way be considered as an admission that such prior art is widely known or forms part of common general knowledge in the field.

SUMMARY OF THE INVENTION The invention provides compositions for reducing an immune condition. In addition, the invention provides methods of administration of an agent, preferably a binding agent COMS ID No: SBMI-01383081 Received by IP Australia: Time 10:41 Date 2005-07-28 28. JuI. 2005 10:35 SheIston IP No.7516 P. -6- (such as an antibody) to an adhesion molecule, particularly an adhesion molecule involved in the extravasion of white blood cells or cancer cells from the blood into the surrounding tissue, to an animal suffering from the immune condition (or predisposed to develop the immune condition). The relatively low dosage of the agent according to the compositions and methods of the invention allow for a reduction in the risk of a treated animal developing antibody-dependent cellular cytotoxicity (ADCC) or antibodymediated toxicity.

According to the first aspect, the present invention provides a method for reducing an immune condition in an animal suffering from an immune condition or predisposed to suffer from the immune condition, wherein the immune condition is inflammation, comprising administering to the animal a therapeutically effective amount of an antibody or antigen binding fragment thereof that specifically binds to a Sialyl Lewis A epitope on an adhesion molecule expressed on the surface of or secreted by a blood-borne cell or expressed on the surface of or released by an endothelial cell lining a blood vessel, wherein the antibody or antigen binding fragment thereof induces an immune response to the antibody or antigen binding fragment thereof that inhibits the interaction between Sialyl Lewis A and its ligand in the animal.

In some embodiments of the first aspect of the invention, the adhesion molecule is a molecule comprising a carbohydrate epitope. In certain embodiments, the carbohydrate 20 epitope is Sialyl Lewis X or Sialyl Lewis A. In some embodiments, the adhesion molecule is a selectin. In preferred embodiments, the selectin is P-selectin or E-selectin.

In certain embodiments of the first aspect of the invention, the immune condition is inflammation. In certain embodiments, the blood-borne cell is a white blood cell. In certain embodiments, the white blood cell is selected from the group consisting of a lymphocyte, a neutrophil, a polyrnorphonuclear leukocyte, and a monocyte.

In certain embodiments of the first aspect of the invention, the immune condition is cancer. In certain embodiments, the blood-borne cell is a cancer cell. In certain embodiments, the cancer cell is selected from the group consisting of a breast cancer cell, a melanoma cell, a liver cancer cell, a lung cancer cell, a leukemic cell, and a lymphoma cell..

In various embodiments of the first aspect, the binding agent is an antibody, such as a monoclonal antibody. Preferably, the binding agent is a murine monoclonal antibody.

mfAA4 4 Fa312 COMS ID No: SBMI-01383081 Received by IP Australia: Time 10:41 Date 2005-07-28 28. Jul. 2005 10:35 Shelston IP No. 7516 P. 6 -7- In certain embodiments of the first aspect of the invention, the immune response results in the generation of an antibody that specifically binds to the binding agent. In certain embodiments, the immune response results in the generation of an antibody that specifically binds to the adhesion molecule. In some embodiments, the immune response comprises a humoral and a cellular immune response.

In various embodiments of the first aspect, the therapeutically effective amount of the binding agent is a dosage of the binding agent that does not induce antibodydependent cellular cytotoxicity (ADCC) in the animal. In certain embodiments, the therapeutically effective amount of the binding agent is a dosage of the binding agent that does not induce antibody-mediated toxicity in the animal. In certain embodiments, the therapeutically effective amount of the binding agent is a dosage of the binding agent that is less than about 8 mg per 30 kg body weight of the animal.

According to the second aspect, the present invention provides a method for reducing an immune condition in an animal suffering from an immune condition or 00" 15 predisposed to suffer from the immune condition, wherein the immune condition is 0000 :inflammation associate with cancer, comprising administering to the animal a therapeutically effective amount of an antibody or antigen binding fragment thereof that specifically binds to Sialyl Lewis A, wherein the antibody or antigen binding fragment thereof induces an immune response to the antibody or antigen binding fragment thereof that inhibits the interaction between Sialyl Lewis A and its ligand in the animal and 00 whreinhe aontiody ora anigem idnaramnlhro.i aal f euigsi 0':":Preferably, the animal is a domesticated. More preferably, the animal is a mammal, 00 .00. such as a humnan.

0 0: In some embodiments of the second aspect of the invention, the adhesion molecule is a molecule comprising a carbohydrate epitope. In certain embodiments, the carbohydrate epitope is Sialyl Lewis X or Sialyl Lewis A. In some embodiments, the adhesion molecule isoa selectin. In preferred embodiments, the selectin is P-selectin or E-selectin.

In certain embodiments of the second aspect of the invention, the immune condition is inflammation. In certain embodiments, the blood-borne cell is a white blood cell. In certain embodiments, the white blood cell is selected from the group consisting of a lymphocyte, a neutrophil, a polymorphonuclear leukocyte, and a monocyte.

WVCI 4 rra" COMS IO No: SBMI-01383081 Received by IP Australia: Time 10:41 Date 2005-07-28 28. Jul. 2005 10:35 Shelston IP No. 7516 P. 7 -8in certain embodiments of the second aspect of the invention, the immune condition is-cancer. In certain embodiments, the blood-borne cell is a cancer cell. In certain embodiments, the cancer cell is selected from the group consisting of a breast cancer cell, a melanoma cell. a liver cancer cell, a lung cancer cell, a leukemic cell, and a lymphoma cell.

In various embodiments of the second aspect, the binding agent is an antibody, such as a monoclonal antibody. Preferably, the binding agent is a murine monoclonal antibody.

In certain embodiments of the second aspect of the invention, the immune response results in the generation of an antibody that specifically binds to the binding agent. In certain embodiments, the immune response results in the generation of an antibody that S*specifically binds to the adhesion molecule. In some embodiments, the immune response comprises a humoral and a cellular immune response.

In various embodiments of the second aspect, the therapeutically effective amount of the binding agent is a dosage of the binding agent that does not induce antibody- S.dependent cellular cytotoxicity (ADCC) in the animal. In certain embodiments, the therapeutically effective amount of the binding agent is a dosage of the binding agent that does not induce antibody-mediated toxicity in the animal. In certain embodiments, the therapeutically effective amount of the binding agent is a dosage of the binding agent that is less than about 8 mg per 30 kg body weight of the animal.

In a certain preferred embodiment of the second aspect of the invention, the composition also comprises a pharmaceutically-acceptable carrier.

According to the invention, agents of the invention, such as binding agents found to e* :specifically bind to an adhesion molecule, may be administered in a therapeutically effective amount to an animal suffering from inflammation, cancer, or predisposed to suffer from inflammation or cancer. For example, a therapeutically effective amount of an antibody that specifically binds to Sialyl Lewis X may be administered with a pharmaceutically-acceptable carrier physiological sterile saline solution) via any route of administration to a patient suffering from inflammation in an attempt to alleviate any resulting disease symptom necrosis). For example, the agent of the invention may be delivered subcutaneously, intravenously, intraperitoneally, intraarterially, intradermally, or intra-muscularly. Pharmaceutically-acceptable carriers and their formulations are well-known and generally described in, for example, Remington's CAA94 an COMS ID No: SBMI-01383081 Received by IP Australia: Time 10:41 Date 2005-07-28 28.MJu. 2005 10~:36 Sheiston IP No. 7516 P. 8 -9- Pharmaceutical1 Sciences (I18th Edition, ed. A. Gennaro, Mack Publishing Co. Easton, PA, 1990).

Unless the context clearly requires otherwise, throughout the dcscription and the claims, the words 'comprise', 'comprising', and the like are to be construed in an inclusive sense as opposed to an exclusive or exhaustive sense; that is to say, in the sense of "including, but not limited to".

v COMS ID No: SBMVI-0133081 Received by IP Australia: Time 10:41 Date 2005-07-28 WO 01/91792 PCT/IB00/02077 BRIEF DESCRIPTION OF THE DRAWINGS Figure 1 is a schematic representation illustrating the different steps in the adhesion cascade between leukocytes and endothelial cells (Dfaz-Gonzalez and Sanchez-Madrid, Immunology Today 19(4): 1-3, 1998).

Figure 2 is a schematic representation of the idiotypic cascade, a non-limiting theory of the present invention, showing reactivity with ligand and/or receptor by mirror image. In this schematic, Abl (the injected antibody) which specifically binds to and blocks the receptor antigen on the surface of a cell a tumor cell) is depicted as having a diamond shape. The injected animal then generates an immune response to Abl resulting in the production of Ab2, depicted in this schematic as having a shape. Ab2 in this schematic specifically binds to the natural ligand (depicted as a solid triangle) of the receptor. The presence of Ab2 results in the production by the immune system of the animal a third antibody, Ab3, which is depicted as having a pentagonal shape that mimicks the natural ligand of the receptor.

Figure 3 is a schematic representation illustrating the use of the enzyme linked immunosorbent assay (ELISA) for the detection of Ab2 antibodies. Note that where the Abl antibody is a murine antibody which is injected into a rat, this ELISA will also detect rat antimouse antibodies (RAMA).

Figure 4 is a schematic representation illustrating the use of ELISA for the detection of Ab3. Note that the CA19-9 antigen bears the Sialyl Lewis A antigen.

Figure 5 is a schematic representation showing SDS-polyacrylamide Gel Electrophoresis of purified monoclonal antibodies (10% acrylamide Tris-HCI under reducing conditions). Molecular weight (MW) is indicated at the left for the standard loaded into Lane 1. The antibodies are as follows: F5 (IgM) (Lane 2; purified by Mannan binding protein affinity chromatography); 6C2C5C12 (IgM) (Lane 3; purified by the euglobulin precipitation method); ALT-4 (also called HB1; IgM) (Lane 4; purified by Mannan binding protein affinity chromatography); Alt-4 (also called HB 1; IgM) (Lane 5; old sample of purified antibody); Flopc-21 (IgG3) (Lane 6; purified by protein A affinity chromatography) ALT-3 (IgG3) (Lane 7; purified by the euglobulin precipitation method); and ALT-3 (IgG3) (Lane 8; old sample of purified antibody).

Figure 6 is a schematic representation showing the binding of various representative, non-limiting murine monoclonal antibodies (7C2C5C12, F5, ALT-3, and Flopc-21 (at the WO 01/91792 PCT/IB00/02077 indicated concentrations) to immobilized tumor antigen CA19-9, a non-limiting representative antigen of the invention.

Figure 7 is a schematic representation showing the binding of various representative, non-limiting murine monoclonal antibodies (7C2C5C12, F5, ALT-3, and Flopc-21 (at the indicated concentrations) to immobilized polyacrylamide-sialyl Lewis A oligosaccharide, a non-limiting representative antigen of the invention.

Figure 8 is a schematic representation showing the inhibitory effect of sialyl Lewis A antigen, a non-limiting representative antigen of the invention, on the binding of Iodine-125 radiolabeled HB 1 (also called ALT-4) antibody, a non-limiting representative therapeutic antibody of the invention, to immobilized CA19-9 antigen, a non-limiting antigen of the invention. Radio-immunoassay plates were coated with CA19-9 at 65 U/mL and incubated with radiolabeled HB1 at 0.33 p.g/mL (a non-saturating amount) and increasing concentrations of Sialyl Lewis A antigen (0-100 gg/ml).

Figure 9 is a schematic representation showing Scatchard analysis of the binding of Iodine-125 radiolabeled HB1 mAb (also called ALT-4), a non-limiting representative therapeutic monoclonal antibody of the invention, to CA19-9 antigen, a non-limiting representative antigen of the invention. Radioimmunoassay strips were coated with CA19-9 antigen at 65 U/mL, and radiolabeled antibody was added to strips at different dilutions ranging from 0.0005 pig/mL to 10.5 gpg/mL. The affinity of HB1 antibody to CA19-9 was determined by measuring the radioactivity of free and bound fractions in individual wells by Gamma counter.

Figures 10A, 10B and 10C are schematic representations showing the inflammatory response in different rat groups for Example I as measured by the caliper method. Eight measurements were performed, and the results are represented as AUC percent change in effect. Figures 10A, 10B and 10C represent the same data, illustrated as a bar graph (Fig.

a column scatter (Fig. 10B), and a whicker box (Fig. Figures 11A, 11B, and 1 1C are schematic representations showing the inflammatory response in different rat groups for Example I as measured by the caliper method. Eight measurements were performed, and the results are represented as percent change in maximum effect. These figures represent the same data, illustrated as a bar graph (Fig. 11A), a column scatter (Fig. 11B), and a whicker box (Fig. 1lC).

WO 01/91792 PCT/IB00/02077 Figures 12A, 12B, and 12C are schematic representations showing the inflammatory response in different rat groups for Example I as measured by the water displacement method. Eight measurements were performed, and the results are represented as AUC percent change in effect. Figures 12A, 12B and 12C represent the same data, illustrated as a bar graph (Fig. 12A), a column scatter (Fig. 12B), and a whicker box (Fig. 12C).

Figures 13A, 13B, and 13C are schematic representations showing the inflammatory response in different rat groups for Example I as measured by the water displacement method. Eight measurements were performed, and the results are represented as percent change in maximum effect. These figures represent the same data, illustrated as a bar graph (Fig. 13A), a column scatter (Fig. 13B), and a whicker box (Fig. 13C).

Figure 14 is a schematic representation of a column scatter graph showing the RAMA.

response with the inflammatory response in different rat groups for Example I. Figs. 10B and 12B are reiterated below Fig. 14 to allow comparison the of the extent of the inflammatory response as measured by the caliper method (Fig. 10B) or the water displacement method (Fig. 12B) with the level of immune response (Fig. 14) Figures 15A, 15B and 15C are schematic representations showing the inflammatory response in different rat groups for Example II as measured by caliper method. Eight measurements were performed, and the results are represented as AUC percent change.

Figures 15A, 15B and 15C represent the same data, illustrated as a bar graph (Fig. 15A), a column scatter (Fig. 15B), and a whicker box (Fig. Figures 16A, 16B, and 16C are schematic representations showing the inflammatory response in different rat groups for Example II as measured by caliper method. Eight measurements were performed, and the results are represented as percent change in maximum effect. These figures represent the same data, illustrated as a bar graph (Fig. 16A), a column scatter (Fig. 16B), and a whicker box (Fig. 16C).

Figures 17A, 17B, and 17C are schematic representations showing the inflammatory response in different rat groups for Example I as measured by water displacement method.

Eight measurements were performed, and the results are represented as AUC percent change.

These figures represent the same data, illustrated as a bar graph (Fig. 17A), a column scatter (Fig. 17B), and a whicker box (Fig. 17C).

Figures 18A, 18B, and 18C are schematic representations showing the inflammatory response in different rat groups for Example II as measured by water displacement method.

WO 01/91792 PCT/IB00/02077 Eight measurements were performed, and the results are represented as percent change in maximum effect. These figures represent the same data, illustrated as a bar graph (Fig. 18A), a column scatter (Fig. 18B), and a whicker box (Fig. 18C).

Figure 19 is a schematic representation of a column scatter graph showing the RAMA response with the inflammatory response in different rat groups for Example II. Figs. and 17B are reiterated below Fig. 19 to allow comparison the of the extent of the inflammatory response as measured by the caliper method (Fig. 15B) or the water displacement method (Fig. 17B) with the level of immune response (Fig. 19) Figures 20A-20H are schematic representations of line graphs showing the correlation between the RAMA response and the inflammatory response at different time measurements in the group of rats treated with HB1 antibody, a non-limiting representative therapeutic antibody of the invention, in Example II. The inflammatory response was measured at liours (Fig. 20A), 2.5 hours (Fig. 20B), 3.5 hours (Fig. 20C), 4.5 hours (Fig. 20D), 5.5 hours (Fig. 20E), 6.5 hours (Fig. 20F), or 7.5 hours (Fig. 20G) after injection of carrageenan. In Fig. 20H, the AUC percent change in effect was plotted against the RAMA (Ab2) response.

Figures 21A, 21B and 21C are schematic representations showing the inflammatory response in different rat groups for Example II as measured by the caliper method. Seven measurements were performed, and the results are represented as AUC percent change in effect. Figures 21A, 21B, and 21C represent the same data, illustrated as a bar graph (Fig.

21A), a column scatter (Fig. 21B), and a whicker box (Fig. 21C).

Figures 22A, 22B, and 22C are schematic representations showing the inflammatory response in different rat groups for Example III as measured by the caliper method. Seven measurements were performed, and the results are represented as percent change in maximum effect. These figures represent the same data, illustrated as a bar graph (Fig. 22A), a column scatter (Fig. 22B), and a whicker box (Fig. 22C).

Figures 23A, 23B, and 23C are schematic representations showing the inflammatory response in different rat groups for Example I as measured by the water displacement method. Seven measurements were performed, and the results are represented as AUC percent change in effect. Figures 23A, 23B, and 23C represent the same data, illustrated as a bar graph (Fig. 23A), a column scatter (Fig. 23B), and a whicker box (Fig. 23C).

Figures 24A, 24B, and 24C are schematic representations showing the inflammatory response in different rat groups for Example III as measured by the water displacement WO 01/91792 PCT/IB00/02077 method. Seven measurements were performed, and the results are represented as percent change in maximum effect. Figures 24A, 24B and 24C represent the same data, illustrated as a bar graph (Fig. 24A), a column scatter (Fig. 24B), and a whicker box (Fig. 24C).

Figure 25 is a schematic representation of a column scatter graph showing the RAMA response with the inflammatory response in different rat groups for Example If. Figures 21B and 23B are reiterated below Figure 20 to allow comparison the of the extent of the inflammatory response as measured by the caliper method (Fig. 21B) or the water displacement method (Fig. 23B) with the level of immune response (Fig. Figures 26A-26G are schematic representations showing the correlation between the RAMA response and the inflammatory response at different time measurements in the group of rats treated with HB1 antibody, a non-limiting representative therapeutic antibody of the invention, in Example III. The inflammatory response was measured at 1.5 hours (Fig. 26A), hours (Fig. 26B), 3.5 hours (Fig. 26C), 4.5 hours (Fig. 26D), 5.5 hours (Fig. 26E), hours (Fig. 26F), or 7.5 hours (Fig. 26G) after injection of carrageenan.

Figures 27A-27G are schematic representations showing the correlation between the RAMA response and the inflammatory response at different time measurements in the group of rats treated with F5 antibody, a non-limiting representative antibody of the invention, in Example III. The inflammatory response was measured at 1.5 hours (Fig. 27A), 2.5 hours (Fig. 27B), 3.5 hours (Fig. 27C), 4.5 hours (Fig. 27D), 5.5 hours (Fig. 27E), 6.5 hours (Fig.

27F), or 7.5 hours (Fig. 27G) after injection of carrageenan.

Figures 28A, 28B and 28C are schematic representations showing the inflammatory response in different rat groups for Example IV as measured by caliper method. Eight measurements were performed, and the results are represented as AUC percent change.

These figures represent the same data, illustrated as a bar graph (Fig. 28A), a column scatter (Fig. 28B), and a whicker box (Fig. 28C).

Figures 29A, 29B, and 29C are schematic representations showing the inflammatory response in different rat groups for Example IV as measured by caliper method. Eight measurements were performed, and the results are represented as percent change in maximum effect. These figures represent the same data, illustrated as a bar graph (Fig. 29A), a column scatter (Fig. 29B), and a whicker box (Fig. 29C).

Figures 30A, 30B, and 30C are schematic representations showing the inflammatory response in different rat groups for Example IV as measured by water displacement method.

WO 01/91792 PCT/IB00/02077 Eight measurements were performed, and the results are represented as AUC percent change.

These figures represent the same data, illustrated as a bar graph (Fig. 30A), a column scatter (Fig. 30B), and a whicker box (Fig. Figures 31A, 31B, and 31C are schematic representations showing the inflammatory response in different rat groups for Example IV as measured by water displacement method.

Eight measurements were performed, and the results are represented as percent change in maximum effect. These figures represent the same data, illustrated as a bar graph (Fig. 31A), a column scatter (Fig. 31B), and a whicker box (Fig. 31C).

Figure 32 is a schematic representation of a column scatter graph showing the RAMA (Ab2) response with the inflammatory response in different rat groups for Example IV. Figs.

28B and 30B are reiterated below Fig. 32 to allow comparison the of the extent of the inflammatory response as measured by the caliper method (Fig. 28B) or the water displacement method (Fig. 30B) with the level of immune response (Fig. 32) Figures 33A, 33B and 33C are schematic representations showing the inflammatory response in different rat groups for Example V as measured by caliper method. Eight measurements were performed, and the results are represented as AUC percent change.

These figures represent the same data, illustrated as a bar graph (Fig. 33A), a column scatter (Fig. 33B), and a whicker box (Fig. 33C).

Figures 34A, 34B, and 34C are schematic representations showing the inflammatory response in different rat groups for Example V as measured by caliper method. Eight measurements were performed, and the results are represented as percent change in maximum effect. These figures represent the same data, illustrated as a bar graph (Fig. 34A), a column scatter (Fig. 34B), and a whicker box (Fig. 34C).

Figures 35A, 35B, and 35C are schematic representations showing the inflammatory response in different rat groups for Example V as measured by water displacement method.

Eight measurements were performed, and the results are represented as AUC percent change.

These figures represent the same data, illustrated as a bar graph (Fig. 35A), a column scatter (Fig. 35B), and a whicker box (Fig. Figures 36A, 36B, and 36C are schematic representations showing the inflammatory response in different rat groups for Example V as measured by water displacement method.

Eight measurements were performed, and the results are represented as percent change in WO 01/91792 PCT/IB00/02077 maximum effect. These figures represent the same data, illustrated as a bar graph (Fig. 36A), a column scatter (Fig. 36B), and a whicker box (Fig. 36C).

Figure 37 is a schematic representation of a column scatter graph showing the RAMA (Ab2) response with the inflammatory response in different rat groups for Example V. Figs.

33B and 35B are reiterated below Figure 37 to allow comparison the of the extent of the inflammatory response as measured by the caliper method (Fig. 33B) or the water displacement method (Fig. 35B) with the level of immune response (Fig. 37) Figures 38A-38H are schematic representations of histograms showing the binding of therapeutic monoclonal antibodies to human neutrophils as determined by single step indirect immunofluorescence flow cytometry (FACS). In Figs. 38A and 38B, colo 205 cells, which are known to express the Sialyl Lewis A epitope, were used as a positive control Figs. 38C- 38H are neutrophils stained with control (no primary antibody) (Fig. 38C), ALT-4 IgM antibody (Fig. 38D), ALT-3 IgG3 antibody (Fig. 38E), 7C2C5C12 IgM antibody (Fig. 38F), IgM antibody (Fig. 38G), and Flopc-21 IgG3 antibody (Fig. 38H).

Figures 39A and 39B are schematic representations showing the instrumental setup and the schematic diagram of the in vitro inhibition of leukocyte rolling and adhesion in microchips channels coated with selectins.

Figure 40 is a schematic representation of a bar graph showing the inhibition of human polymorphonuclear leukocyte (PMNL) rolling in microchip channels coated with recombinant E-selectin, a non-limiting representative adhesion molecule of the invention, by the various indicated agents. The graph bars show the percentage of human PMNL rolling.

Figure 41 is a schematic representation showing the inhibition of human PMNL rolling in microchip channels coated with recombinant P-selectin, a non-limiting representative adhesion molecule of the invention, by the various indicated agents. The graph bars show the percentage of human PMNL rolling.

WO 01/91792 PCT/IB00/02077 DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS The present inventors have developed methods and therapeutic compositions for reducing inflammation. The methods and therapeutic compositions of the invention are useful as analytical tools and as therapeutic tools. The invention also provides methods and therapeutic compositions which may be manipulated and fine-tuned to fit the condition(s) to be treated while producing fewer side effects. Standard reference works setting forth the general principles of the technology described herein include Ausubel et al., Current Protocols in Molecular Biology, John Wiley Sons, New York, 1994; Sambrook et al., Molecular Cloning: A Laboratory Manual 2d Ed., Cold Spring Harbor Laboratory Press, Plainview, New York, 1989; Kaufman et al. Handbook of Molecular and Cellular Methods in Biology and Medicine, CRC Press, Boca Raton, 1995; and McPherson, Ed., Directed Mutagenesis: A Practical Approach, IRL Press, Oxford, 1991. Standard reference works setting for the general principles of immunology and inflammation include Gallin et at, Inflammation: Basic Principles and Clinical Correlates, Raven Press, New York, 1988; Kuby, Immunology. 3 d ed., W.H. Freeman, New York, 1997; Coligan et al. (Eds.), Current Protocols in in Immunology, John Wiley Sons, New York, 1991; and Hurley, J.V., Acute Inflammation, 2 d ed., Churchill Livingstone, New York, 1983.

The published patent and scientific literature referred to herein establishes knowledge that is available to those with skill in the art: The issued U.S. patents, allowed applications, published foreign patent applications, and references, including GenBank database sequences, that are cited herein are hereby incorporated by reference to the same extent as if each was specifically and individually indicated to be incorporated by reference. Any inconsistency between these publications and the present disclosure shall be resolved in favor of the present disclosure.

In a first aspect, the invention provides a method for reducing an immune condition in an animal suffering from the immune condition or predisposed to suffer from the immune condition, comprising administering to the animal a therapeutically effective amount of a binding agent that specifically binds to an adhesion molecule expressed on the surface of or secreted by a blood-borne cell or expressed on the surface of or released by an endothelial cell lining a blood vessel, wherein the binding agent induces an immune response in the animal to the binding agent. Preferably, the binding agent is administered with a pharmaceutically-acceptable carrier.

WO 01/91792 PCTIIBOO/02077 In a second aspect, the invention provides a therapeutic composition for reducing an immune condition in an animal suffering from the immune condition or predisposed to suffer from the immune condition, comprising a therapeutically effective amount of a binding agent that specifically binds to an adhesion molecule expressed on the surface of or secreted by a blood-borne cell or expressed on the surface of or released by an endothelial cell lining a blood vessel, wherein the binding agent induces an immune response in the animal to the binding agent. Preferably, the composition further comprises a pharmaceutically-acceptable carrier.

In a third aspect, the invention provides a therapeutic composition comprising an agent that is capable of reducing inflammation and reducing cancer in an animal when administered to the animal in a therapeutically effective amount. Preferably, the composition further comprises a pharmaceutically-acceptable carrier.

In a fourth aspect, the invention provides a method for reducing an immune condition in an animal suffering from cancer or inflammation or predisposed to suffer from cancer and inflammation, comprising administering to the animal a therapeutically effective amount of an agent that is capable of reducing inflammation and reducing cancer in an animal As used herein, by "agent" is meant a molecule of any size which may be naturally occurring or synthetic. Thus, an agent includes, without limitation, a chemical a protein, a carbohydrate, a lipid, a nucleic acid, an acid, a base, a synthetic polymer, and a resin.

As used herein, by "immune condition" is meant cancer and/or inflammation. By "predisposed to suffer from an immune condition "is meant an animal that has a genetically or environmentally caused predisposition to suffer from an immune condition, but is not yet suffering from the immune condition. For example, the animal may have a certain type of MHC haplotype rendering it more susceptible to develop an immune condition, such as rheumatoid arthritis, but has not yet developed the condition. Another example is an animal which has been exposed to intense sunlight, but has not yet developed a sunburn. Another example is an animal which has been exposed to a carcinogen, but has not yet developed cancer.

In certain embodiments of the first, second, third, and fourth aspects of the invention, the immune condition is cancer. In accordance with the invention, by "cancer" is meant the excessive growth of an abnormal cell in an animal resulting in the migration of metastatic WO 01/91792 PCT/IB00/02077 cells via the blood stream from the original anatomical site of the original abnormal cell to other parts in the body. In accordance with the invention, the detection of a secondary site of cancer caused by the migration of a metastatic cell is not required for an animal to be diagnosed as suffering from cancer. Rather, an animal a human) may have a cell growth biopsied. Upon a physician's inspection of the biopsied tissue and an assessment that the biopsied cells are likely malignant capable of metastasis), the animal may be treated in accordance with the invention.

One non-limiting cancer of the invention is melanoma. Other non-limiting cancers of the invention include breast cancer, colorectal cancer, bladder cancer, leukemia, lymphoma, cervix cancer, prostate cancer, testicular cancer, liver cancer, lung cancer, ovarian cancer, and pancreatic cancer.

In certain embodiments of the first, second, third, and fourth aspects of the invention, the immune condition is inflammation. In accordance with the invention, by "inflammation" is meant a local response to cellular injury that is marked by any or all of capillary dilatation, leukocyte infiltration, redness, heat, and pain and that serves as a mechanism initiating the elimination of the inflammation-inducing antigen and of damaged tissue. The term includes acute inflammation, chronic inflammation, and excessive inflammation, such as that leading to inflammatory disease.

Acute inflammation exhibits a rapid onset and is of short duration. The characteristic signs of an acute localized inflammatory response generally include swelling, redness, heat, pain and loss of function (Kuby, Immunology, 3 rd ed., W.H. Freeman, New York, pages 361-377, 1997). Infiltration ofleukocytes into the tissue peaks within the first 6 hours of an acute inflammatory response, and most of them disappear from the inflamed area within 24- 48 hours (Kuby, supra).

Chronic inflammation develops during persistence of an antigen due to infection or various pathologic conditions. The characteristic hallmark of chronic inflammation is the formation of granuloma. Many different types of cells may be found in the extravascular tissues in areas of chronic inflammation, including any or all of neutrophils, cosinophils, macrophages, epithelioid cells, plasma cells, lymphocytes, and fibroblasts (Hurley, J.V., Acute Inflammation, 2 n d ed., Churchill Livingstone, New York, pages 135-144, 1983).

Excessive inflammation, either secondary to abnormal recognition of host tissue as "foreign" or deviation from an otherwise normal inflammatory response, leads to WO 01/91792 PCT/IBOO/02077 inflammatory disease (GaUlin et al., Inflammation: Basic Principles and Clinical Correlates, Raven Press, New York, 1988). Most forms of acute and chronic inflammation are amplified as well as propagated as a result of the recruitment of humoral and cellular response (Gallin et al., supra).

As used herein, by "reducing an immune condition" is meant the ability of an agent a binding agent) of the invention to alleviate the symptoms of and/or prevent the onset of an immune condition in an animal suffering from the immune condition (or predisposed to suffer from the immune condition an animal exposed to the sun which has not yet developed a sunburn; an animal who has been exposed to a carcinogen which has not yet developed cancer; or an animal genetically predisposed to develop an immune condition which has not yet developed the immune condition)) when administered to that animal.

Preferably, the alleviation of symptoms and/or prevention of onset is reduced in a treated animal as compared to the immune condition in an animal to which an agent of the invention has not been administered. Preferably, the reduction in the immune condition in an animal to which has been administered a binding agent of the invention is at least 10% lower as compared to an untreated animal; more preferably, the reduction is at least 25% lower; still more preferably, the reduction is at least 50% lower; even more preferably, the reduction is at least 75% lower; and most preferably, the reduction in the immune condition in an animal to which has been administered a binding agent of the invention is at least 100% lower as compared to an untreated animal an animal to which a binding agent of the invention has not been administered). Methods for determining the amount of an immune condition in an animal, where the condition is inflammation, include, without limitation, visual detection of redness and measurement of swelling by the caliper method or by the water displacement method described below. Methods for determining the amount of an immune condition in an animal, where the condition is cancer, include, without limitation, detection of the presence of a metastatic cancer cell and detection of cancer cell-induced angiogenesis.

In certain embodiments of the first, second, third, and fourth aspects of the invention, the animal suffering from an immune condition is preferably a domesticated animal including, without limitation, domesticated fowl ducks, geese, chickens, turkeys, Cornish hens, and ostriches), domesticated livestock cattle, llamas, elephants, camels, pigs, and sheep), domesticated pets horses, cats, dogs, ferrets, hamsters, and guinea pigs); and laboratory animals primates baboons, Rhesus monkeys) and rodents mice, rats)).

WO 01/91792 PCT/IB00/02077 In a preferred embodiment of the first, second, third, and fourth aspects of the invention, the animal suffering from the immune condition is a mammal. In a preferred embodiment of the first and second aspects of the invention, the animal suffering from the immune condition is a human.

As used herein, by the term, "induces an immune response" is meant that a cellular and/or humoral immune response is induced or increased in an animal to which has been administered a binding agent of the invention, such that the immune response is directed toward the administered binding agent. Thus, in preferred embodiments of the first, second, third, and fourth aspects of the invention, the immune response comprises both a humoral and a cellular immune response.

The presence of such an immune response can be detected by any of the well known immune response detection methods including, without limitation, detection of the presence of a T cell a cytotoxic T cell or helper T cell) whose T cell receptor specifically binds to the binding agent and/or to the adhesion molecule (in the context of the major histocompatibility complex), detection of the presence of a B cell whose B cell receptor surface-expressed IgM) specifically binds to the binding agent and/or to the adhesion molecule, and detection of the presence of an antibody (of any serotype) which specifically binds to the binding agent and/or to the adhesion molecule.

In certain embodiments of the first, second, third, and fourth aspects of the invention, the immune response results in the generation of an antibody that specifically binds to the binding agent. Such an antibody that specifically binds to the binding agent is generated as a result of an immune response in the treated animal to the administered binding agent. One non-limiting theory explaining such an immune response is the idiotypic cascade first described by Jerne (Jerne, Annals of Innunology 125C: 373-389, 1974). This theory, as illustrated for an idiiotypic cascade of antibodies, where the first antibody specifically binds to a tumor associated antigen, is illustrated on Figure 2 (Kingsbury et al., Leukemia 12: 982-991, 1998).

Adapting this theory to the present invention, an administered binding agent an antibody) that specifically binds to an adhesion molecule is designated Abl. Abl reacts with epitopic determinants on the adhesion molecule. The animal to which has been administered a binding agent of the invention generates an immune response to that binding agent. Where the immune response comprises antibodies that bind to the binding agent, these antibodies are WO 01/91792 PCT/IB00/02077 designated Ab2. Some Ab2 antibodies (namely the Ab2p, recognize the adhesion moleculebinding site of Abl and resemble the original adhesion molecule epitope recognized by Abl.

Other Ab2 antibodies recognize sites present elsewhere on the variable region, and may (Ab2y) or may not (Ab2a) interfere with the binding site (Steinitz et al., Journal of Immunology 141: 3516-3522, 1988).

Furthermore, the idotypic network theory postulated that each Ab2 might serve as the antigen for an additional antibody (Ab3). This Ab3 antibody, like the original binding agent (Abl) specifically binds to the adhesion molecule to which Abl specifically bound. Thus, in certain preferred embodiments of the first and second aspects of the invention, the immune response results in the generation of an antibody that specifically binds to the adhesion molecule.

In one non-limiting example, the injection of a selectin E-selectin) specific binding agent (Abl) into an animal suffering from an immune condition, where the immune condition is inflammation, could inhibit leukocyte white blood cell) rolling on the endothelial cells lining the walls of a blood vessel through two different mechanisms. The first mechanism of this non-limiting example is dependent on the direct effect of Ab I that will act to block the selectin on either the white blood cell surface or endothelial cell membrane, and make it unavailable for binding by a selectin ligand sialyl Lewis A)bearing cell The effect of Abl would be direct but unfortunately would also be short- term due to the rapid clearance of Abl from the circulation (Abl usually remained in the circulation for several hours or few days only). The second mechanism is dependent on the induction of anti-idiotypic (Ab2) and anti-anti-idiotypic (Ab3) Abs. Since Ab2 would mimic a selectin, it will inhibit through its binding to selectin molecules the interaction between selectins and their natural ligands. Ab3 will act as Abl and compete with the naturally existing carbohydrte selectin ligand in being bound by its selectin and as a result, inhibit the interaction between selectins and their ligands through competitive inhibition.

In another non-limiting example, the injection of a carbohydrate selectin ligand sialyl Lewis A) specific binding agent (Abl) into an animal suffering from an immune condition, where the immune condition is cancer, could inhibit metastatic cancer cell melanoma cell) rolling on the endothelial cells lining the walls of a blood vessel through two different mechanisms. The first mechanism is dependent on the direct effect of Abl that will act to block selectin ligand on either white blood cell surface or endothelial cell membrane, and make it unavailable for binding by a selectin-bearing cell. The direct effect of Abl WO 01/91792 PCT/IB00/02077 would be direct but short- term. The second mechanism is dependent on the induction of anti-idiotypic (Ab2) antibodies which mimic the selectin ligand and anti-anti-idiotypic (Ab3) antibodies which mimic the Abl antibody that specifically bound to the selectin ligand. Ab2 would mimic a selectin ligand, thus inhibiting cancer cell rolling on endothelial cells and extravasation through its binding to selectin molecules. Ab3 will inhibit cancer cell rolling and extravasaion by competing with the naturally existing selectin molecule in binding to carbohydrate selectin ligand.

The effect of Ab2 and Ab3 the indirect effect of Abl) would likely to be more efficient than the direct effect of Abl for the inhibition of leukocyte or cancer cell migration, since Ab2 and Ab3 antibodies are produced by the treated animal and remain in the circulation for a considerable period of time (usually several months).

As used herein, the term "therapeutically effective amount" is used to denote a dosage of binding agent effective to reduce an immune condition in the treated animal and/or a dosage of binding agent effective to induce an immune response to the binding agent in the treated animal Preferably, such administration should be intravenous, intra-arterial, subcutaneous, parenteral, or transdermal. Preferably, a binding agent of the invention is administered at a dosage of less than about 8 mg per 30 kg body weight of the animal to be treated; preferably less than about 5 mg per 30 kg body weight; preferably less than about 3 mg per 30 kg body weight; still more preferably from about 0.5 mg to about 2 mg per 30 kg body weight; still more preferably from about 0.5 mg to about 1.5 mg per 30 kg body weight; and most preferably, a binding agent of the invention is administered at a dosage of about 1 mg per 30 kg body weight of the animal to be treated.

In certain embodiments of the first, second, third, and fourth aspects of the invention, the therapeutically effective amount of a binding agent is a dosage of the binding agent that, when administered to an animal, does not induce antibody-dependent cellular cytotoxicity (ADCC) in the treated animal. In certain preferred embodiments of the first and second aspects of the invention, the therapeutically effective amount of a binding agent is a dosage of the binding agent that is the maximum amount of binding agent that, when administered to an animal, does not induce antibody-dependent cellular cytotoxicity (ADCC) in the treated animal. In these embodiments, ADCC may be assessed by incubating 5Cr-labeled tumor cells with a binding agent according to the invention and adding fresh human peripheral blood mononuclear cells (PBMCs), followed by incubation for four hours and then WO 01/91792 PCT/IB00/02077 measurement of specific lysis (as determined by 5 1 Cr release). ADCC is absent if specific lysis is less than In certain embodiments of the first, second, third, and fourth aspects of the invention, the therapeutically effective amount of a binding agent is a dosage of the binding agent that, when administered to an animal, does not induce antibody-mediated toxicity in the treated animal. In certain preferred embodiments of the first and second aspects of the invention, the therapeutically effective amount of a binding agent is a dosage of the binding agent that is the maximum amount of binding agent that, when administered to an animal, does not induce antibody-mediated toxicity in the treated animal. By "antibody-mediated toxicity" is meant clinical toxicity, such as abnormal serum chemistries, impaired renal function, signs and symptoms of serum sickness, or anaphylaxis. Antibody-mediated toxicity is well-known to immunologist and described, for example, in Abbas et al., Cellular and Molecular Immunology, 3 rd ed., W. B. Saunders Co., Philadelphia, PA 1997 (see particularly pages 425- 434). In certain embodiments, a single such dosage will therapeutically treat the animaL In other embodiments, the treatment may be ongoing, administration of a dosage of a binding agent four times a year for three or more years. In one example, the administration of a dosage of a binding agent will be performed one injection per month for about three months to years.

In accordance with the invention, the term, "blood-borne cell," means any cell that can be carried by the blood stream. Thus, the definition includes bone marrow derived cells such as red blood cells and white blood cells which include, without limitation, lymphocytes (both T and natural killer cells, neutrophils (also called polymorphonuclear leukocytes or PMNL), cosinophils, basophils, monocytes, megakaryocytes, and platelets. Also included as blood-borne cells are metastatic cancer cells which have migrated (or are attempting to migrate) from the site of the original abnormal cell.

Thus, in those embodiments of the first, second, third, and fourth aspects of the invention, where the immune condition is inflammation, the blood-borne cell is a white blood cell. In certain embodiments, the white blood cell is, without limitation, a lymphocyte, a platelet, a granulocyte a a neutrophil, eosinophil, or a basophil), or a monocyte.

In those embodiments of the first, second, third, and fourth aspects of the invention, where the immune condition is cancer, the blood-borne cell is a cancer cell. Any cancer cell that is carried by the blood stream is included in the invention. In certain embodiments, the WO 01/91792 PCT/IB00/02077 cancer cell is, without limitation, a breast cancer cell, a melanoma cell, a liver cancer cell, a lung cancer cell, a leukemic cell, and a lymphoma cell.

As used herein, by "adhesion molecule" is meant a molecule which is expressed on the surface of or released by either a blood-borne cell or a cell an endothelial cell) that lines a blood vessel and which can form an association with another adhesion molecule expressed on the surface of either a blood-borne cell or a cell lining a blood vessel. By "released by" means an adhesion molecule that is either cleaved from the cell surface of the indicated cell, secreted by the indicated cell, or released from the indicated cell upon lysis of that cell. Preferably, an adhesion molecule of the invention is a molecule expressed on the surface of or released by either on a blood-borne cell or an endothelial cell lining a blood vessel that forms an association with a second adhesion molecule on a blood-borne cell or an endothelial cell lining a blood vessel, such that association formed is between a blood-borne cell-expressed or released adhesion molecule and an endothelial cell-expressed or released adhesion molecule. Preferably, an adhesion molecule of the invention is one that is involved in the extravasion of white blood cells or cancer cells from the blood into the surrounding tissue. Preferably, the definition of "adhesion molecule" excludes the B cell receptor, the T cell receptor, and/or the signalling molecules comprising the B cell receptor complex Ig-a) and the T cell receptor complex CD3). The site on an adhesion molecule of the invention which is specifically bound by the binding agent is called an "epitope." Non-limiting examples of adhesion molecules include the molecules which are members of the integrin family of proteins including, without limitation, LFA-1 (CD1la/CD18), VLA-4 (CD49d/CD29), and Mac-1 (CDllb/CD18). Adhesion molecules also include those molecules which are members of the immunoglobulin gene family of proteins including, without limitation, ICAM-1, B7, CD4, and CD28. Adhesion molecules also include those molecules which are members of the selectin family of molecules including, without limitation, P-selectin ELAM-1, CD62E), E-selectin (ELAM-1, CD62E) and L-selectin (CD62L) (Bevilacqua and Nelson, Journal of Clinical Investigation 91: 370- 387, 1993. Thus, in certain embodiments of the first and second aspects of the invention, the adhesion molecule is a selectin, such as P-selectin, E-selectin, or L-selectin.

Adhesion molecules of the invention also include glycoproteins, such as PSGL-1 (Pselectin glycoprotein ligand 1) and molecules mucins or mucoproteins) comprising carbohydrate epitopes, including the Sialyl Lewis A (SLe(a)) carbohydrate epitope and the Sialyl Lewis X (SLe(x)) carbohydrate epitope. Thus, in certain embodiments of the first and WO 01/91792 PCT/IB00/02077 second aspects of the invention, the adhesion molecule of the invention is a molecule comprising a carbohydrate epitope, such as a molecule comprising a Sialyl Lewis A epitope.

Preferably, a molecule comprising a carbohydrate epitope is specifically bound by a selectin molecule.

In various embodiments of the third and fourth aspects of the invention, the agent is a binding agent that specifically binds to an adhesion molecule.

As used herein, by "binding agent" is meant a molecule or macromolecule that specifically binds to an adhesion molecule either in water, under physiological conditions in an animal's body), or under conditions which approximate physiological conditions with respect to ionic strength, 140 mM NaCI, 5 mM MgClz. By "specifically binds" is meant a binding agent of the invention recognizes and forms a covalent association or, preferably, a non-covalent association with an adhesion molecule of the invention, but does not substantially recognize and form a covalent or non-covalent association with other molecules in a sample, proteins that are not adhesion molecules. Likewise, an adhesion molecule of the invention bound by a binding agent of the invention that specifically binds to that adhesion molecule is said to be "specifically bound" by that binding agent. Preferably, a binding agent of the invention that specifically binds to an adhesion molecule of the invention forms an association with that adhesion molecule with an affinity of at least 106 M more preferably, at least 107 M' 1 even more preferably, at least 108 and most preferably, at least 10 9

M.

In certain embodiments, a binding agent of the invention is a peptide, a peptidomimetic, or a carbohydrate. By "peptide" is meant a molecule comprised of a linear array of two or more amino acid residues connected to each other in the linear array by peptide bonds. By "peptidomimetic" is meant a non-peptide molecule that mimics the structure and/or function of a peptide. By "carbohydrate or oligosaccharide" is meant a molecule comprising sugar residues. Non-limiting binding agents that are carbohydrates include SLe(x) oligosaccharide (Buerke et al., Journal of Clinical Investigation 93: 1140- 1148, 1994; Lefer et al., Circulation 90: 2390-2401, 1994), and SLe(a) oligosaccharide.

Non-limiting binding agents of the invention include antibodies. Thus, in certain embodiments of the first, second, third, and fourth aspects of the invention, the binding agent is an antibody, such as a monoclonal antibody. In certain preferred embodiments, the binding agent is a murine monoclonal antibody.

WO 01/91792 PCT/IB00/02077 For example, the monoclonal antibody of the invention, HB1 (also called ALT-4), is a murine IgM monoclonal antibody (mAb) secreted by the HB 1 hybridoma cell line. HB1 specifically binds to the tumor marker, CA19-9, which comprises the carbohydrate epitope Sialyl Lewis A (Mukae et al., Am. Rev. Respir. Dis. 148(3): 744-751, 1993). Immunoassay analysis has revealed that HBE1 also specifically binds to Sialyl Lewis A. HB1 hybridoma cells were maintained in standard media (RPMI-1640 supplemented with 2 mM L-glutamine, U/ml penicillin and 50 U/ml streptomycin, 10% v/v fetal bovine serum (FBS)).

Another non-limiting antibody of the invention is HB2 (also called ALT-3), a IgG3 murine monoclonal antibody secreted by the HB2 hybridoma cell line. HB2 specifically binds to the tumor marker CA19-9, and has also been shown to specifically bind to sialyl Lewis A. HB2 hybridoma cells were maintained in standard media.

Yet additional non-limiting binding agents that are antibodies include PB1.3, an anti- P selectin monoclonal antibody (Weiser et al., Shock 402-407, 1996; Yamada et al., European Journal of Pharmacology 346: 222-224, 1998); ARP2-41, an anti-rat-P-selectin mAb (Tojo et al., Glycobiology 463-9, 1996; Ohnishi et al. Immunopharmacology 34: 161-170, 1996); BBIG-E5, an anti-E-selectin mAb (Altavilla et al., European Journal of Pharmacology 270: 45-51,1994); DREG-200, an anti-L-selectin mAb, (Ma et al., Circulation 88: 649-658, 1993); MEL-14, an anti-L-selectin mAb (MEL-14) (Pizcueta and Luscinskas, American Journal of Pathology 145(2): 461-467, 1994); anti- SLe(x) mAb (Yoshinori et al., Journal of Pathology 180: 305-310, 1996); anti- SLe(x) mAb (anti-human and rat) (clone no.

commercially available from BD Pharmingen, San Jose, CA); anti- SLe(x) mAb (clone no. CSLEX; commercially available from Becton Dickinson Immunocytometry Systems, San Jose, CA); anti- SLe(x) mAb (clone no. CSLEX1; commercially available from Signet Laboratories, Inc., Dedham, MA); anti-PSGL-1 (CD162) antibodies commercially available from Biodesign IntL, Saco, ME clone no. 3E2.25.5(PL1) (Catalog No.

P91090M) and clone no. 5D8.8.12 (PL2) (Catalog No. P91209M) and the various anti-SLe(a) mAb commercially available from Biodesign IntL, Saco, ME clone no. 241 (Catalog No. M37241B); clone no. 59 (95-330-07) (Catalog No. M37059M); clone no. 62 (Catalog No. M37062M); clone no. 67 (Catalog No. M37067M); clone no. 192 (Catalog No.

M37192M); and clone no. 239 (Catalog No. M37239M)).

Yet additional non-limiting binding agents of the invention include the anti-human Eselectin and anti-human P-selectin antibodies commercially available from R D Systems (Minneapolis, MN, USA).

WO 01/91792 PCT/IB00/02077 In certain embodiments, the binding agents of the invention comprises a complementarity determining region (CDR) of an antibody or a T cell receptor that specifically binds to an adhesion molecule of the invention. By "complementarity determining region (CDR) of an antibody" is meant a portion of an antibody that specifically binds to an epitope, including any framework regions necessary for such binding, and which is preferably comprised of a subset of amino acid residues encoded by the immunoglobulin gene heavy chain V, D, and J regions, the immunoglobulin gene light chain V and J regions, and/or any combinations thereof. By "complementarity determining region (CDR) of a T cell receptor" is meant a portion of a T cell receptor that specifically binds to an epitope in context of the major histocompatibility complex), including any framework regions necessary for such binding, and which is preferably comprised of a subset of amino acid residues encoded by the T cell receptor P chain gene V, D, and J regions, the T cell receptor a chain gene V and J regions, and/or any combinations thereof. Preferably, the CDR of the invention is derived from a human or a murine antibody.

Given the numerous antibodies disclosed herein, one of skill in the art are enabled to make a variety of antibody derivatives, wherein such antibody derivatives are also included as binding agents of the invention. For example, Jones et al. (Nature 321: 522-525, 1986) disclose replacing the CDRs of a human antibody with those from a murine antibody. Marx, J.L. (Science 229(4712): 455-456, 1985) discusses chimeric antibodies having murine variable regions and human constant regions. Rodwell, J.D. (Nature 342(6245): 99-100, 1989) discusses lower molecular weight recognition elements derived from antibody CDR information. Clackson, T.P. (Br. J. RheumatoL 30 Suppl 2:36-39, 1991) discusses genetically engineered monoclonal antibodies, including Fv fragment derivatives, single chain antibodies, fusion proteins, chimeric antibodies, and humanized rodent antibodies.

Reichman et al. (Nature 332: 323-327, 1988) discloses a human antibody on which rat hypervariable regions have been grafted. And Verhoeyen et al. (Science 239: 1534-1536, 1988) teaches the grafting of a mouse antigen binding site onto a human antibody.

In addition, given the numerous antibodies disclosed herein, those of skill in the art are enabled to design and produce peptidomimetics having binding characteristics similar or superior to such complementarity determining regions, wherein such peptidomimetics are included as binding agents of the invention. Peptidomimetics are well known and described, for example, in Horwell, Bioorg. Med. Chem 4: 1573-1576, 1996; Liskamp et al., Recl.

WO 01/91792 PCT/IB00/02077 Trav. Chim. Pays-Bas. 1: 113, 1994; Gante et aL, Angew. Chem. Int. Ed. Engl. 33: 1699, 1994; and Seebach et al., Helv. Chim. Acta 79: 913, 1996.

The binding agents of the present invention are preferably purified binding agents.

By "purified" is meant that a binding agent is at least 60%, preferably at least 75%, even more preferably at least 90%, and most preferably at least 95% free from contaminants, such as endotoxins.

In a non-limiting example, where the binding agents of the invention are monoclonal antibodies, to purify the antibodies HB1 and HB2), the antibodies were purified from ascites. For ascites preparation, adult mice (age 6 weeks) were primed by injecting 0.5 ml of pristane (2,6,10,14-tetramethyldecanoic acid) into the peritoneum Two weeks later the mice were injected Lp., with 5 x 106 hybridoma cells HB1 or HB2) resuspended in ml of PBS. Ascetic fluid was tapped from mice 2 to 3 times in due time, and the collected fluid was incubated for 1 hour at room temperature and transferred to 4°C overnight. The fluid was centrifuged at 2000 rpm for 15 minutes and the supernatant was stored at -20 °C.

The monoclonal antibodies were then purified and concentrated. As various methods for purifying and concentrating antibodies are known to those of skill in the art, the following purification methods are non-limiting examples of methods that can be used for purifying antibodies from ascites. It should be noted that these methods can also be used to purify and concentrate antibodies from the supernatant of hybridoma cells grown in vitro under standard tissue culture conditions.

For mannan binding protein (MBPP affinity chromatography purification, the ascetic fluid containing the IgM was dialyzed against binding buffer (10 Mm Tris, 1.25 M NaCI, mM CaC12, and 0.02 Sodium azide (NaN 3 at a pH of after which the ascetic sample was diluted 1: 1 v/v with binding buffer, and filtered with 0.22 mm filter. The MBP column (commercially available from Pierce, Rockford, IL, USA) was prewashed with 2 column volume (20 ml) of elution buffer (10 mM Tris, 1.25 M NaCI, 2mM EDTA and 0.02% NaN 3 and then equilibrated with 4 column volume (40 ml) of binding buffer. The ascetic fluid (maximum 1.5 ml/ 5 ml of gel) was applied to the column, allowed to completely enter the gel, and incubated at 4 °C for 30 minutes. The column was washed with 9 column volumes of the binding buffer to remove the unbound proteins. The wash was monitored for the presence of proteins by measuring the absorbance at 280 nm, using the binding buffer as a reference.

The column was removed from the cold and incubated with the elution buffer at room WO 01/91792 PCT/IB00/02077 temperature for 1 hour, after which the column was washed with the elution buffer, and minimum of 14 fractions were collected each with a volume of 3 ml. The elution of IgM was monitored for the presence of proteins by spectrophotometer at 280 run using the elution buffer as a reference. At the completion of the purification the column was washed with 2 column volume of deionized water and then by 2 column volumes of 4 °C binding buffer and stored at 4 oC.

The euglobulin precipitation method is a non-chromatographic. method used for the purification of both murine IgG 3 and IgM mAbs, which takes advantage of their euglobulin properties. This method was performed as essentially described by (Garcia-Gonzalez et at, Journal of Imiunological Methods 111: 17-23, 1988). Briefly, CaC12 was added to the ascetic fluid (final concentration, 25 mM) to generate fibrin formation. When the clot was formed, it was removed by paper filtration. Then the filtered ascetic fluid was dialyzed for 2 hours at 20 °C (IgG3), or for 15 hours at 4 oC (IgM) against 100x volume of demineralized water (pH The ascetic fluid was centrifuged in a BECKMAN L8-55 ultracentrifuge at 22,000 x g for 30 minutes and the precipitate was recovered and suspended in 1M NaCl/0.1 M Tris-HC1 pH 8. Dialysis and precipitation were repeated twice. Purified pellet with a high lipid content was mixed with 1.7 M NaCI and centrifuged for 3 hours at 27,000 x g. Lipid supernatant was discarded, the clarified mAbs solution was dialyzed against 0.1M Tris HC1, 1M NaCI pH 8, centrifuged at 22,000 x g for 30 minutes. The purified pellet was suspended in phosphate buffered saline (PBS).

For Protein A affinity chromatography purification, a 5 ml Protein A column (commercially available from Pierce, Rockford, IL, USA) was washed with 2 column volumes of elution buffer (0.1 mM Glycine, ImM NaC1, 0.001 tween 20, pH Then the column was equilibrated with 5 column volumes of binding buffer (50 mM Tris-HC1, ImM NaC1, 0.001 tween 20, pH The ascetic sample containing IgG 3 antibody was diluted 1:1 v/v with binding buffer and loaded to the column at a rate of 0.75 ml/ minute, then the column was washed with 50 ml of binding buffer, at a rate of 1.5 ml minute. The collected fractions containing unbound proteins were checked by spectrophotometer at 280 nm, using the binding buffer as a reference. The column was washed with 15 ml of elution buffer. Then the eluted fractions were collected at a rate of 1 ml /minute and adjusted to a pH of 7 using mM Tris-HCl. Elution of bound proteins was monitored by spectrophotometer at 280 nm, using the elution buffer as a reference.

WO 01/91792 PCT/IB00/02077 For removal of endotoxins from purified immunoglobulins, a 10 ml Detoxi-Gel column (commercially available from Pierce, Rockford, IL, USA) was regenerated by washing the gel with 5 column volumes of 1% sodium deoxycholate. Then the column was washed with 3 column volumes of pyrogen-free water. The purified and concentrated immunoglobulin was loaded to the column, and then the sample was collected by gravity flow using PBS as elution buffer.

Before use, the ultrafilter YM-30 membrane (MW cutoff 30,000 Dalton) (commercially available from Fisher Scientific, Nepean, ON, Canada) was floated with distilled water for one hour, changing the water three times. Then the membrane was mounted in an ultrafiltration cell and rinsed with distilled water at 20 psi (3.7 atm) for at least minutes. The samples of purified Igs were loaded in the cell, and the sample flow through the ultrafilter membrane was operated under a maximum pressure of 40 psi (4.7 atm). The antibody solution was concentrated approximately 10x and then washed at least three time using PBS.

The quantification of purified F5 IgM, 7C2C5C12 IgM and Flopc-21 IgG 3 mAbs was done by ELISA where plates were coated with goat anti-mouse Ig (commercially available from Sigma Chemical Co., St. Louis, MO, USA). Note that this ELISA was also used to detect the presence of either Ab2 antibody in a treated animal of the invention, or the presence of rat anti-mouse antibody, since all of the injected antibodies are murine antibodies injected into rats. A schematic diagram of this assay is shown on Figure 3.

The quantification of HB 1 IgM and HB2 IgG3 mAbs was done by ELISA where the plates were coated with CA19-9 antigen. Note that this ELISA was also used to detect the presence of Ab3 antibodies. A schematic diagram of this assay is shown on Figure 4.

For each tested mAb, an isotype matched control mAb was used as a standard.

Clarified ascites containing the mouse IgG3k monoclonal antibody Flopc-21 was obtained from SIGMA Chemical Co. (St Louis, MO, USA). Control F5 antibody is murine IgM monoclonal antibody secreted by the F5 hybridoma cell line (commercially available from the American Type Culture Collection, Maryland, USA). Control 7C2C5C12 antibody is a murine IgM monoclonal antibody which specifically binds to Trichinella spiralis and which is secreted by the 7C2C5C12 hybridoma cell line (commercially available from the American Type Culture Collection, Maryland, USA). Both F5 hybridoma cells and 7C2C5C12 hybridoma cells were maintained in standard media (RPMI-1640 supplemented with 2 mM WO 01/91792 PCT/IB00/02077 L-glutamine, 50 U/ml penicillin and 50 U/ml streptomycin, 10% v/v fetal bovine serum

(FBS)).

For the ELISA assay for determining the concentration or the binding activity of mAbs, microtiter strips were coated with 100 ml of PBS containing either 2.5 jlg/ml of goat anti-mouse Ig (whole molecule), 75 U/ml of CA19-9 antigen (commercially available from Altarex Corp., Edmonton, Alberta, Canada), or 2.5 g.g/ml of polyacrylamide-SLe(a) (commercially available from Altarex Corp., Edmonton, Alberta, Canada) and incubated overnight at 4 oC. The coating solution was discarded and 150 pjl of blocking buffer (2% sucrose, 2% BSA, 0.06% thimerosal in PBS) was added to each well in order to block the non-specific binding sites on the wells. After one hour of incubation at room temperature the plates were washed three times with PBST. 100 pl of binding buffer containing mAbs was added in different dilutions. All wells were incubated for 1 hour at room temperature, followed by three washings with PBST (PBS containing 0.1% Tween-20, pH Then 100 p.l of binding buffer containing goat anti-mouse Ig -HRP labeled (commercially available from Southern Biotechnology Associates Inc., Birmingham, AL, USA) was added to each well at a dilution of 1/5000 v/v, incubated for 1 hour, and followed by three washings with PBST. The activity of bound mAbs was determined by adding a 100 .tl of ABTS (Peroxidase solution B and ABTS peroxidase substrate, 1:1 v/v dilution) to each well (ABTS Peroxidase solution and ABTS peroxidase substrate commercially available from LifeTechnologies GIBCO BRL (Burlington, Ontario). The optical density was measured at dual wavelength, 405 and 492 nm using an ELISA reader (Titertek multiskan Plus MK II).

An SDS-polyacrylamide gel electrophoresis was used to check the purity of each of the purified mAbs. Ab samples were diluted 1:4 v/v with SDS-PAGE sample buffer (0.5 M Tris-HCI at pH 6.8, 20% glycerol, 10% w/v SDS, 10% b- mercaptoethanol and 0.5 bromphenol blue) and heated for 4 minutes at 95 oC. The Tris-HCL ready gel sandwich acrylamide) (commercially available from BioRad, Hercules, CA, USA) was inserted into the Mini-PROTEAN II cell clamp assembly and aligned properly. Then the gel sandwich was attached to the inner core of the Mini-PROTEAN II cell (commercially available from BioRad). The upper and the lower buffer chambers were filled with approximately 200 ml of lx running buffer Tris base, 1.45% glycine, 0.1% SDS, pH 6 pl of molecular weight markers in sample buffer was applied to one of the wells, while the antibody samples, each with a volume of 30 pl (1.5-5 jig of antibody) were applied WO 01/91792 PCT/IB00/02077 to the rest of the wells in the gel The core assembly was inserted into the lower buffer tank and the gel was run at 200 V constant voltage for approximately 45 minutes. The gel was then removed from the gel sandwich, immersed in staining solution (10% acetic acid, 0.025% Coomassie BlueG-250, methanol 40%) for 1.5 hour, and destained in destaining solution (10% acetic acid, 40% methanol) until the desired destaining was reached.

Antibodies of the invention were purified and then resolved by SDS-polyacrylamide gel electrophoresis. As shown in Figure 5, antibodies purified by the mannan binding protein affinity chromatograph (lanes 2 and by the euglobin precipitation method (lanes 3 and 7), and by protein A affinity chromatography (lane 6) are "purified" for the purposes of the present invention (compare to lanes 5 and 8 for old samples of purified monoclonal antibodies.

In the non-limiting embodiment where the binding agent of the invention is a monoclonal antibody, the binding of the various murine monoclonal antibodies to tumor antigen CA19-9 and to sialyl Lewis A was determined. Using the ELISA assay described above and schematically depicted in Figure 4 (using CA19-9 coated plates), the various purified monoclonal antibodies were tested for binding to purified CA19-9. A range of mAb concentration from 0 to 0.64 mg/ml was used. As can be seen in Figure 6, for both HB1 (ALT-4) and HB2 (ATL-3) mAbs, even at a concentration of only 10 ng/ml, maximum binding to CA19-9 was observed which is likely to be due to the presence of multiple sialyl Lewis A epitopes within the CA19-9 antigen. As expected, the two control mAbs F5 and 7C2C5C12 did not bind to CA19-9 antigen. Surprisingly, however, the control mAb Flopc- 21 that was used in the same concentration range bound to CA19-9 with an observed dose effect.

In order to determine more specifically the epitope recognized by HB1 and HB2, a second ELISA assay (as described above) was performed which used the carbohydrate antigen SLe(a) conjugated to polyacrylamide in order to carry a direct binding assay. As shown in Figure 7, both HB1 (ALT-4) and HB2 (ALT-3) mAbs recognized the carbohydrate epitope SLe(a). In addition, Figure 7 demonstrated that Flopc-21 mAb is directed against a different CA19-9 epitope determinant than the SLe(a) epitope recognized by HB2 and HB 1 and, thus, does not interact with SLe(a) carbohydrate oligosaccharide.

The specificity of HB 1 mAb for SLe(a) was also studied. To do this, HB I was first radiolabeled with 25 I (iodinated). 50 glg of HB1 mAb was mixed with approximately 600 WO 01/91792 PCT/IB00/02077 I.Ci of Na 12sI. 10 ml of 2.5 mg/ml of chloramine-T (CT) (commercially available from Sigma Chemical Co.) was added to the reaction vial and gently shaken for 30 seconds. Then, Pl of 5 mg/ml sodium metabisulfate (Na-Met) was added to the mixture and shaken gently for 15 seconds. Finally, 20 pl of 1M sodium iodide (Nal) was added and the whole mixture was gently shaken for 5 seconds. The reaction mixture was loaded to the Desalting Econopac 10 DG chromatographic column (commercially available from BioRad, Hercules, CA, USA) pre-washed three times with 0.5% BSA in PBS. The vial was rinsed with 100 pl of PBS and transferred to the column. The column was then washed with 0.5 ml PBS fractions 12 to 14 times. Each elute was collected separately in numbered tubes. The radioactivity of elutes was monitored by a Geiger counter. The fractions containing the mAbs were pooled, labeled, placed in lead containers, and stored at 4 OC. The quantification of 1 25 labeled HB1 mAb in the pooled fractions was done by ELISA as described above (and depicted in Figure 4) using plates coated with CA19-9. A nonradioactive control HB 1 mAb was used as standard.

For competitive inhibition assay, RIA strips coated with 50 p.1 of PBS containing a U/ml of tumor antigen CA19-9 were incubated overnight at 4 OC. After discarding the coating solution, the non-specific binding sites were blocked with 150 pl/well of blocking buffer (2% sucrose, 2% BSA, 0.06% thimerosal in PBS). The wells were incubated for 1 hour at room temperature, and then washed with PBST three times. Dilutions of SLe(a) antigen (commercially available from Oxford GlycoSciences Inc., MA, USA) were made serially in binding buffer BSA, 0.02% thimerosal in PBS) from 0 to 100 gpg ml and mixed with iodinated HB I mAb. A nonsaturating concentration (0.33 pg/ml) ofiodinated HB1 (ALT-4) mAb was chosen in order to obtain a final count per minute concentration of approximately 40,000 c.p.mJwell. 100 .l of each dilution was added to different wells and allowed to incubate for 1.5 hours. After that the supernatant was discarded, the strips were washed with PBST three times and broken down to individual wells and the radioactivity was measured (bound fraction).

As shown in Figure 8, only at high concentrations, namely 50 ig /ml and 100 tig/ml, was SLe(a) antigen able by competitive inhibition to reduce the binding of HB1 to CA19-9 antigen by 35% and 60% respectively. The requirement for such a high concentration of Sle" to inhibit the binding of HB1 mAb to CA19-9 could be due to the fact that HB1 Ab is an IgM and therefore has 10 binding sites for each SLe(a) molecule. In addition, the affinity of HB 1 WO 01/91792 PCT/IB00/02077 Ab for the SLe(a) epitope carried by CA19-9 is likely to be higher than the affinity of HB1 Ab for soluble SLe(a). Such difference in affinity may be due to a more stabilized threedimensional configuration of the SLe(a) carried by CA19-9 and/or to a longer sequence recognized by HBI within CA19-9 molecule.

The affinity of HB1 (ALT-4) mAb to Cal9-9 antibody was next determined by scatchard analysis using iodinated HB1 mAb. To do this, radio-immunoassay (RIA) strips were coated with 100 pl of PBS containing 65 U/ml of CA19-9 antigen overnight at 4 °C.

After discarding the coating solution, the nonspecific binding sites were blocked with 150 tl/well of blocking buffer sucrose, 2% BSA, 0.06% thimerosal in PBS). The wells were incubated for 1 hour at room temperature with gentle shaking, and then washed three times with PBST (PBS, 0.1% Tween-20, pH Each strip was then incubated with a range of concentration from 0.005 to 10.5 pgg/ml of 1125 labeled HB1 antibody in 100 pl1 of binding buffer BSA, 0.02% thimerosal in PBS) at room temperature for 1 hour. Then, 50 pC of the HB I supernatant was taken from each well and the radioactivity was measured (free fraction) gamma counter. The strips were again washed three times with PBST, broken into individual wells and the radioactivity of each well was measured (bound fraction) by Gamma counter.

Thus, the affinity of iodinated HB1 antibody to CA19-9 antigen was determined by measuring the radioactivity of free and bound fractions in individual wells by Gamma counter. As shown in Figure 9, the Kd value from this experiment was calculated to be equal to 0.94 nM.

The following examples are intended to further illustrate certain preferred embodiments of the invention and are not limiting in nature. Those skilled in the art will recognize, or be able to ascertain, using no more than routine experimentation, numerous equivalents to the specific substances and procedures described herein. Such equivalents are considered to be within the scope of this invention, and are covered by the following claims.

Example I Male Sprague Dawley (SD) rats age 6 weeks, weight 275-300 grams, were injected five times with 250 mg/rat of either keyhole limpet hemocyanin (KLH)-conjugated HB 1 mAb, control KLI-I-conjugated F5 mAb, or were simply received PBS injection via intraperitoneal route.

WO 01/91792 PCT/IB00/02077 Serum collection was routinely done prior to each immunization. The tails of rats were cut slightly at the tip and the blood was collected in serum separator microtainers.

During this procedure the rats were anaesthetized with ethyl ether. The collected blood was centrifuged at 300 x g for five minutes; serum was separated, and used to test for the detection of RAMA and Ab3 responses. The animals were kept under care until inflammation was induced and measured.

The mAbs were conjugated to KLH in order to enhance their immunogenicity.

Chemical conjugation is usually very efficient but is likely to decrease the binding activity of the mAbs. For each conjugation, we therefore determined the remaining binding activity of the KLH-mAbs. This was done by direct ELISA as described above and schematically depicted in Figure 4 using CA19-9 coated plates.

To conjugate antibodies with keyhole limpet hemocyanin (KLH), 1 mg of either HB1 mAb or F5 mAb (control) was suspended in PBS solution was mixed with 1 mg of KLH (commercially available from SIGMA Chemical Co., St Louis, MO, USA) and 2 ml of glutaraldehyde (final concentration, The mixture was incubated for 1 hour at room temperature and then 75 mg of glycine was added to the mixture. The mixture was stirred for an addition 1 hour and then dialyzed against distilled water for three days. The binding activity of the mAbs to CA19-9 antigen was measured before and after conjugation with KLH and compared by ELISA using CA19-9 antigen-coated plates.

Rat anti-mouse antibody (RAMA) and Ab3 responses were measured between each injection. The ELISA assays used for the measurement of Ab2 (RAMA) and Ab3 humoral responses are described above and illustrated in Figures 3 and 4, respectively.

The rats were then subjected to acute inflammation in the right hind paw by injection of carrageenan (carrageenan lambda type IV (gelatin, vegetable, Irish moss); commercially available from Sigma Chemical Co.) five days after the last injection of antibody. To induce inflammation in the rat right hind paw (intraplantary), 0.05 ml of 1% carrageenan in 0.9 NaCI as injected into the rats five days after the last immunization with antibody KLH conjugated HB 1, F5, or PBS only). The animals were lightly anaesthetized with ethyl ether for the injections and for inflammation measurements.

Paw edema was used as a characteristic sign of inflammation, and was quantitated at regular interval of time after carrageenan injection, with a total of 8 measurements. Paw edema was measured by two different methods. The caliper method was used to measure WO 01/91792 PCT/IB00/02077 paw thickness, and water displacement method to measure paw volume. The thickness of rat paw was measured in inches by a caliper device before and after carrageenan injection at desired time intervals. The paw volume was measured by immersing the paw up to the tibiotarsae articulation in a cylinder filled with water, and the fluid volume replaced by immersed paw was measured before and after carrageenan injection at desired time intervals.

Both paw thickness and paw volume measurements of the inflammatory response results were analyzed in the same way. The measurements were plotted as area under the curve (AUC) percent change in effect and as percent change in maximum effect. "Percent change in effect" means the percent change in inflammatory response in each rat from time zero to each consecutive time of inflammation measurement. "AUC percent change in effect (area under the curve)" means the sum of the percent changes in inflammatory response from time zero to each consecutive time of inflammation measurement for each rat. The average of AUC percent change in effect was calculated per rat group. "Percent change in maximum effect" means the percent change in inflammatory response from time zero to the time where maximum inflammation has occurred in each rat.

Paw thickness measurement results are illustrated as AUC percent change in effect in Figures 10A, 10B, and 10C; and as percent change in maximum effect in Figures 11A, 11B, and 11C. Paw volume measurement results are illustrated as AUC percent change in effect in figures 12A, 12B, and 12C; and as percent change in maximum effect in Figures 13A, 13B, and 13C.

For both paw thickness and paw volume measurements, the least edema was observed in HB1 group, but this difference in change in effect, measured by caliper method 0.631) or by water displacement method 0.655), was not statistically significant.

Ab3 was not detected in any of the rat groups tested. The results obtained for the measurement of the RAMA immune response are shown in Figure 14. The RAMA response was detected in only one rat in the group of rats treated with HB1 mAb (rat 5, Figure The PBS only-injected group did not receive any mAb injection and consequently had no RAMA response (Figure 14). The absorbance obtained by ELISA corresponds therefore to background level (Figure 14). The F5 control mAb-injected group showed absorbance values above background level. These values correspond to anti-isotypic and/or anti-allotypic immune responses (the assay performed with HB 1 coated plates cannot measure the antiidiotypic response specific for F5 antibody). The HB1 therapeutic mAb-injected group WO 01/91792 PCT/IB00/02077 showed absorbance values above background level that corresponds to either anti-isotypic, anti-idiotypic or anti-allotypic immune response or a combination of them, among which only the idiotypic response will have the therapeutic effect.

It is worth noting that in the HB 1 group, one rat (rat 5) had a high RAMA response (see Fig. 14). Of particular interest, this particular rat did not develop any inflammatory response (see Figures 10B and 12B).

Example II Male Sprague Dawley rats (age six weeks) were injected four times with 500 tg/rat of either KLH-conjugated HB1, control KLH-conjugated F5, control KLH-conjugated 7C2C5C12 mAbs, or have simply received PBS injection via i.p. route.

RAMA and Ab3 responses were measured between each injection. Induction and measurement of inflammation were done by the same methods as described in Example I.

Paw edema was observed and measured at intervals of time.

Paw thickness and paw volume measurement results were analyzed and plotted as described in Example I. Paw thickness measurement results are illustrated as AUC percent change in effect in Figure 15A, 15B, and 15C; and as percent change in maximum effect in Figures 16A, 16B, and 16C. Paw volume measurement results are illustrated as AUC percent change in effect in Figures 17A, 17B, and 17C; and percent change in maximum effect in Figures 18A, 18B, and 18C.

For both paw thickness and paw volume measurements, the least edema was observed in HBl-injected group, but this difference in change in effect, measured by caliper method 0.134) or by water displacement methods (0.073) was not statistically significant.

Ab3 was not detected in any of the rat groups tested. A very low RAMA immune response was detected in the control groups (F5 and 7C2C5C12). The HB1-injected group was determined using the method depicted in Figure 3 to be the only group with a high RAMA immune response (Figure 19). Despite the fact that the group treated with HB1 antibody had the highest immune response, no significant correlation was found between the RAMA response and the therapeutic efficacy as shown in Figure 19.

The inflammatory responses of the HB 1 injected rats at different measurement times was potted as percent change in effect against the RAMA (Ab2) response. As shown in WO 01/91792 PCT/IB00/02077 Figures 20A-20H, the correlation between the therapeutic efficacy and RAMA in HB1 group was not found to be significant at any time of the inflammation measurements.

Example II1 Male Sprague Dawley rats (age six weeks) were injected four times with 500 mg/rat of either HB1, control F5 mAbs mixed with QUIL A adjuvant (commercially available from Calbiochem, San Deigo, USA), or have simply received PBS injection via subcutaneous route. RAMA and Ab3 responses were measured between each injection at regular intervals.

Induction and measurement of inflammation was done by the same methods as described in Example I. Paw edema was observed and measured at regular intervals of time.

Paw thickness and paw volume measurement results were analyzed and plotted as in Example I. Paw thickness measurement results are illustrated as AUC percent change in effect in Figures 21A, 21B, and 21C; and as percent change in maximum effect in Figures 22A, 22B, and 22C. Paw volume measurement results are illustrated as AUC percent change in effect in Figures 23A, 23B, and 23C; and as percent change in maximum effect in Figures 24A, 24B, and 24C.

For both paw thickness and paw volume measurements the least edema was observed in HB1 group, but this difference in change in effect measured by caliper method 0.253) or by water displacement method 0.358) was not statistically significant.

Ab3 was not detected in any of the rat groups tested. RAMA immune response was detected in the therapeutic HB 1 group, and to a lesser extent in the control F5 group as shown in Figure 25 using the method as depicted in Figure 3.

The extent of inflammatory response was correlated with the extent of the level of RAMA immune response in HB1 group as shown in Figures 26A-26G. Of particular interest is the demonstration in HB 1 rat group of a good correlation between RAMA immune response and the inflammatory response, i.e. the higher the RAMA immune response, the lower the inflammatory response. Surprisingly, some correlation was also observed between the extent of the inflammatory response and the RAMA response in the group treated with mAb as shown in Figures 27A- 27G.

WO 01/91792 PCT/IB00/02077 Example IV Male Sprague Dawley rats (age six weeks) were injected four times with 500 gpg/rat of either HBI mAb or have simply received PBS injection via intravenous route. RAMA and Ab3 responses were measured between each injection at regular intervals. Induction and measurement of inflammation was done by the same methods as in experiment 1. Paw edema was observed and measured at regular intervals of time.

Paw thickness and paw volume measurement results were analyzed and plotted as in Example I. Paw thickness measurement results are illustrated as AUC percent change in effect in Figures 28A, 28B, and 28C; and as percent change in maximum effect in Figures 29A, 29B, and 29C. Paw volume measurement results are illustrated as AUC percent change in effect in Figures 30A, 30B, and 30C; and as percent change in maximum effect in Figures 31A, 31B, and 31C.

For both paw thickness and paw volume measurements, the least edema was observed in HB1 group, but this difference in change in effect measured by caliper method 0.111) or by water displacement method 0.188) was not statistically significant.

Ab3 was not detected in any of the rat groups tested.

As shown in Figure 32, the RAMA immune response was detected (using the method depicted in Figure 3) in one rat that belonged to the therapeutic HB 1 group. AUC percent change in effect from the measurements of caliper and water displacement methods showed that one rat in the therapeutic HB 1 group had responded with minimal inflammatory response as shown in Figures 28B and 30B, respectively. Therefore, the extent of the inflammatory response as shown in Figures 28B and 30B was compared to the level of RAMA immune response in each rat in Figure 32. In fact, the same rat that showed the highest RAMA immune response as shown in Figure 32 also showed the least inflammatory response measured by both caliper and water displacement methods as shown in Figures 28B and respectively.

Example V Rats were injected four times with 500 mg/rat of either KLH-conjugated HB2, control KLH-conjugated Flopc-21 mAbs, or have simply received PBS injection via i.p. route.

RAMA and Ab3 immune responses were measured between each injection. Induction and WO 01/91792 W T/OIBO/0277 measurement of inflammation were done by the same methods as described in Example I.

Paw edema was observed and measured at regular intervals of time.

Paw thickness and paw volume measurement results were analyzed and plotted as described in Example I. Paw thickness measurement results are illustrated as AUC percent change in effect in Figures 33A, 33B, and 33C; and as percent change in maximum effect in Figures 34A, 34B, and 34C. Paw volume measurement results are illustrated as AUC percent change in effect in Figures 35A, 35B, and 35C; and as percent change in maximum effect in Figures 36A, 36B, and 36C.

For both paw thickness and paw volume measurements the least edema was observed in HB 1 group, but this difference in change in effect measured by caliper method (P=0.074) or by water displacement method 0.065) was not statistically significant.

Ab3 was not detected in any of the rat groups tested in Example V. Using the method depicted in Figure 3, all tested groups showed minimal (background) RAMA immune response as shown in Figure 37.

Example VI The binding of HB 1 (ALT-4), HB2 (ALT-3), and control monoclonal antibodies (IgM), 7C2C5C12 (IgM), and Flopc-21 (IgG3) to PMNLs and lymphocytes was quantitated by FACScan and fluorescent microscopy. The binding ofHB1, HB2, and control mAbs to HUV-EC-C cells was also examined by fluorescent microscopy.

Human umbilical vein endothelial cells (HUV-EC-C) is an endothelial cell line derived from the vein of a normal, human umbilical cord, obtained from American Type Culture Collection (Maryland, USA). HUV-EC-C cells were maintained in MCDB 131 media supplemented with 10 ng/ ml of HEGF, 12 mg/ ml of BBE, 1 mg/ ml of hydrocortisone, 5 v/v FBS, 0.05 mg/ ml ofGentamycin sulfate, and 0.05 mg/ ml of amphotericin B.

To isolate human white blood cells, 6 ml of Histopaque -Ficoll solution was added to a 15 ml test tube, and then a 6-ml of heparin-treated fresh human blood was carefully layered on top of the gradient. Tubes were centrifuged at 1700 rpm (450 x g) for 35 minutes at room temperature. The band at the upper interface (mononuclear cells) was retrieved (5 ml) and mixed with 10 ml of 0.45% NaCI and 20 ml of PBS (8 mM Di-sodium hydrogen WO 01/91792 PCT/IB00/02077 orthophosphate, 3 mM potassium dihydrogen orthophosphate, 0.14 M NaCI). The tubes were centrifuged at 1500 rpm (400 g) for 15 minutes at 21 The pellet was retrieved and washed three times with 10 ml of 3 mM EDTA, 1% FBS in PBS solution without magnesium and without calcium and centrifuged at 800 rpm (250 x g) for 10 minutes. The pellet was suspended in 2 ml of RPMI 5% fetal bovine serum (FBS). In the case of isolating human PMNL, the band at the lower interface was retrieved and treated in the same way as with monouclear cells. The purified cell pellet (mononuclear or PMNL cells) was suspended at Ix 7 cells/ml in RPMI media, 5% FBS and kept at 37 °C until used. Cell viability was determined by exclusion of 0.4% trypan blue.

For FACS analysis, 1x10 6 white blood cells or HUV-EC-C washed in PBS, 1% bovine serum albumin (BSA), were incubated with primary antibody HB1, HB2, 7C2C5C12, or Floppc-21) at a concentration of 5 jpg/ ml in PBS, 1% BSA. The incubation was performed at 4 °C and after 45 minutes the cells were washed three times with PBS, 1% BSA. The cells were then incubated with fluorescein-isothiocyanate (FITC)-conjugated goat anti-mouse antibody at 4 OC for 45 minutes and washed three times. The stained cells were resuspended in PBS, 1% BSA. Fluorescence associated with the cells was analyzed either by FACScan flow cytometry (Becton Dickinson, Mountain View, CA) or by fluorescence microscopy. As negative control cells were incubated according to the same procedure but without primary antibody.

Examples of histograms obtained by FACS analysis are shown in Figures 38A-38H.

Colo 205 cells, which were used as a positive control (Figs. 38A and 38B), are known to express the Sialyl Lewis A epitope on their surface. Colo 205 cells are a human adenocarcinoma cell line derived from metastatic colon cancer that produces CA19-9 antigen.

Colo 205 cells were obtained from the American Type Culture Collection (Maryland, USA), and were maintained in standard media (RPMI-1640 supplemented with 2 mM L-glutamine, U/ml penicillin and 50 U/ml streptomycin, 10% v/v fetal bovine serum (FBS)).

As can be seen in Figures 38C-38H, all tested mAbs did not show significant binding to human neutrophils. None of the antibodies tested showed significant binding to rat neutrophils nor to HUVEC cells.

WO 01/91792 PCT/IB00/02077 Example VI The in vitro inhibition of leukocyte rolling on microchips by the therapeutic antibodies of the invention was next determined. For these studies, a microchip glass wafer device was used that consists of channels in which in vitro cell rolling and adhesion can be observed under flow conditions, the process that mimics the in vivo cell rolling and adhesion events.

To investigate the rolling and adhesion of cells, a 10 cm x 10 cm glass wafer with a series of simple mixer flow manifolds was used in this assay. The mixer flow manifold consisted of 50 microns in depth and 300 microns in width channels with a flow region downstream. These channels are structured in similar way to capillaries and therefore, the use of such channels allows the observation of cell rolling and adhesion in vitro that mimics the in vivo cell rolling and adhesion events. The microchip was secured into an aluminum microchip holder, and the microchip reservoirs were glued onto the microchip with epoxy resin. The channels were conditioned with concentrated nitric acid (HNO 3 2 M of sulfuric acid (H 2 S0 4 and 1 M of sodium hydroxide (NaOH) (10 minutes each) before and after each experiment. The channels were then coated with 20 jtg/ml of recombinant human E-selectin or P-selectin (commercially available from R D Systems, Minneapolis, MN, USA) for 2 hours at 37 0 C to mimic the surface of a vessel wall endothelial lining. A microsyringe pump that operated as a negative pressure source was used to drive the cells through the channels at a flow rate of 2.5 ml/min for the 50 micron deep device. This flow rate ensured a high enough shear rate (800 s 1 to mimic blood flow in a vascular capillary.

The channels were monitored using a Reichert Microstar (25X 280 x 220 mm field of view) and JVC color CCD camera mounted on the microscope (see Figure 39A). The experiments were recorded onto tapes with a JV VHS recorder.

A Ix 10 7 cells/ml solution of human PMNL or lymphocytes (isolated as described in Example VI) suspended in RPMI, 5% FBS was pumped to the channel through an injection route and allowed to run at an optimum flow rate for the purpose of achieving the rolling step. Since each channel had two sides, one side of each channel-was used to test the agent under study and the other side of the channel was used as a control for cell rolling only without the addition of any agents (see Figure 39B). The cell suspensions added to different sides of the channel were regulated by a luminal fluidic flow that allowed these cell suspensions to continue running at different sides of the channel and prevented cell diffusion from one side of the channel to the other.

WO 01/91792 PCT/IB00/02077 The following agents were tested at a volume of 10 tl and at a dilution of 1:10 v/v in RPMI and 5% FBS: fresh human serum (negative control), fresh rat serum, HB1 rat serum; and F5 rat serum. 10 p.1 of 100 tig/ml of anti-human E-selectin antibody (commercially available from R D Systems, Minneapolis, MN, USA) was used as positive control for Eselectin coated channels. Serum of rats immunized with HB 1 monoclonal antibody from Example II was obtained from rats who showed a high RAMA response (rat 2, 4, 5, 6, 7, and 8, collectively). Serum of rats immunized with F5 monoclonal antibody from Example III was obtained from rats who showed a high RAMA response (rat 4, 6, and 7, collectively). Once the rolling step was achieved, 10 pl of each of the above mentioned agent was added to different channels. The pump sucked the added sample into the channel to start the inhibition step. The images of cell rolling and inhibition steps were captured at different times and the number of cells was counted in both sides of the channel Non-adherent cells appeared as streaks on the video image. The cell counts were conducted on a PC computer installed with a perception video board and image Pro Plus software.

The same agents were also tested on P-selectin coated channels under the same conditions as with E-selectin coated channels. For P-selectin coated channels, anti-human Pselectin antibody (commercially available from R D Systems, Minneapolis, MN, USA) was used as a positive control Since there was no consistent rolling of rat leukocytes on the recombinant human Eor P-selectin coated channels and since rat E- and P-selectins were not available, the assay was carried only with human leukocytes (and not with rat leukocytes). The images of cell rolling and inhibition steps were captured at different times and the rolling cells were counted on both sides of the channel (see Figures 39A and 39B). The effect of each of the tested agents was determined as the percentage of rolling human PMNL from those in the control channel (100%) in which no agents were added.

As shown in Figures 40 and 41, respectively, normal human serum did not cause a reduction in human PMNL rolling on human E-selectin or human P-selectin coated channels.

The same experiment performed with normal rat serum instead of normal human serum revealed by microscopic observation that such treatment resulted in an increased adherence of a small number of leukocytes on both E- and P- selectin coated channels. This effect however was very mild and did not affect the rolling of cells on either E- or P- selectin coated channels as shown in Figures 40 and 41, respectively.

WO 01/91792 PCT/IB00/02077 Anti-E- and anti P-selectin mAbs were used to demonstrate effective rolling inhibition of PMNL on E-selectin (Fig. 40) and P- selectin (Fig. 41) coated channels, respectively.

There was only 1.4 of rolling cells observed with the addition of anti-E- selectin mAb, as compared to the side with the control rolling cells as shown in Figure 40, and 14.4% rolling cells with the addition of anti-P-selectin mAb as shown in Figure 41. Therefore, the rolling of human PMNL leukocytes on recombinant human E- and P- selectin coated channel was inhibited significantly by anti-E- and anti-P- selectin mAbs control" in Figs. 40 and 41).

It was of particular importance to observe that the addition of 1:10 dilution of the RAMA positive serum obtained from rats immunized with HB 1 antibody (rat 2, 4, 5, 6, 7 and 8 collectively, Example III) caused a significant inhibitory effect on PMNL rolling on both human E-selectin and human P- selectin coated channels ("ALT-4 rat serum Ab2 in Figs. 41 and 42, respectively). In fact, the HB1 rat serum the ALT-4 rat serum from rats who showed a positive RAMA response) was able to reduce the rolling of PMNL on Eselectin coated channels to 0% resulting in a 100 inhibition 0.001) (see Fig. 40). The same rat serum resulted in a 76 inhibitory effect on P-selectin coated channels 0.001), since it was able to reduce the rolling of PMNL on P-selectin coated channels from 100 to 23.2 (see Fig. 41). In opposition to normal serum, no change in cell adherence or cell morphology was observed with the addition of rat serum immunized with HB 1 antibody.

Unexpectedly, the RAMA positive control F5 rat serum also showed some inhibition of the rolling of PMNL leukocytes on recombinant human E- and P-selectin coated channels rat serum Ab2+" in Figs. 40 and 41). However, this inhibition was considerably less marked than the one observed with the therapeutic monoclonal antibody HB 1. The addition of serum obtained from rats immunized with F5 antibody who were positive for RAMA response (rats 4, 6 and 7 collectively, Example III) caused a 45 reduction in PMNL rolling on E-selectin coated channels 0.001); and a 33 reduction in PMNL rolling on P-selectin coated channels 0.001) as shown in Figures 40 and 41 respectively.

Example VIII A rodent model of melanoma is used to determine the effect of the binding agents of the invention in reducing melanoma. A murine melanoma cell line, such as clone B16 (commercially available as Catalog No. CRL-6322 from the American Type Culture WO 01/91792 PCT/IB00/02077 Collection, Manassas, VA) is injected subcutaneously or intra-peritoneally into syngeneic mice C57BL/6J mice) or immunodeficient nude mice (both commercially available from The Jackson Laboratory, Bar Harbor, ME) at an established primary site in an amount sufficient for the melanoma cells to establish tumor growth at the primary tumor site in the mice. A first set of mice is injected, and the time needed for metastasis of the melanoma cells to occur is recorded.

The experimental set of rats is injected with the melanoma cells. At the time metastasis is known to occur based on the results obtained from the first set of mice, the experimental set is divided into different groups of animals, each of which is administered a therapeutically effective amount of the various binding agents of the invention including, without limitation, HB1, HB2, ARP2-41, DREG-200, MEL-14, BBIG-E5, and anti-sialyl Lewis X antibodies via a subcutaneous, intraperitoneal, or intravenous injection. Control antibodies include 7C2C5C12 and Mice injected with binding agents of the invention that specifically bind to adhesion molecules expressed either on the cell surface of the melanoma cells or on the cell surface of endothelial cells lining blood vessel walls are found to have a reduced number of tumor formation at secondary sites sites distinct from the primary tumor site) than those mice injected with the control antibodies.

Claims (1)

1. 28. Jul. 2005 10:36 Shelston IP No. 7516 P. 9 -47 THE CLAIMS DEFINING THE INVENTION ARE AS FOLLOWS:- 1. A method for reducing an immune condition in an animal suffering from an immune condition or predisposed to suffer from the immune condition, wherein the immune condition is inflammation, comprising administering to the animal a therapeutically effective amount of an antibody or antigen binding fragment thereof that specifically binds to a Sialyl Lewis A epitope on an adhesion molecule expressed on the surface of or secreted by a blood-borne cell or expressed on the surface of or released by an endothelial cell lining a blood vessel, wherein the antibody or antigen binding firagment thereof induces an immune response to the antibody or antigen binding fragment thereof that inhibits the interaction between Sialyl Lewis A and its ligand in the animal. 2. The method of claim 1, wherein the adhesion molecule is a molecule comprising a carbohydrate epitope. 3. The method of claim 1 or claim 2, wherein the blood-borne cell is a white blood 15 cell. S•4. The method of claim 3, wherein the white blood cell is selected from a lymphocyte, a platelet, a granulocyte, and a monocyte. The method of any one of claims 1 to 4, wherein the antibody is a monoclonal antibody. 20 6. The method of any one of claims 1 to 5, wherein the antibody or antigen binding fragment thereof is xenogeneic. 7. The method of any one of claims 1 to 6, wherein the immune response results in the generation of an antibody that specifically binds to the antibody or antigen binding S• fragment thereof. 8. The method of any one of claims 1 to 6, wherein the immune response results in the generation of an antibody that specifically binds to the adhesion molecule. 9. Themethod of any one of claims 1 to 8, wherein the immune response comprises a humoral and a cellular immune response. The method of any one of claims 1 to 9, wherein the animal is a domesticated animal. 11. The method of any one of claims 1 to 9, wherein the animal is a human. COMS ID No: SBMI-01383081 Received by IP Australia: Time 10:41 Date 2005-07-28 28.MJu. 2005 10:36 Sheiston IP No. 7516 P. 48 12. The method of any one of claims I to 11, wherein the therapeutically effective amount of the antibody or antigen binding fragment thereof is a dosage that does not induce antibody-dependent cellular cytotoxicity (ADCC) in the animal. 13. The method of any one of claims 1 to 12, wherein the therapeutically effective amount of the antibody or antigen binding fragment thereof is a dosage that does not induce antibody-mediated toxicity in the animal. 14. The method of any one of claims i to 12, wherein the therapeutically effective amount of the antibody or antigen binding fragment thereof is a dosage that is less than about 8 mg per 30 kg body weight of the animal. 15, The method of any one of claims 1 to 14, wherein the inflammation results from an immnune reaction against an autoantigen. :00 :16. A method for reducing an immune condition in an animal suffering from an immne condition or predisposed to suffer from the immune condition, wherein the immune condition is inflammation associate with cancer, comprising administering to the animal a therapeutically effective amount of an antibody or antigen binding fragment *thereof that specifically binds to Sialyl Lewis A, wherein the antibody or antigen binding 0 fragment thereof induces an immune response to the antibody or antigen binding fragment thereof that inhibits the interaction between Sialyl Lewis A and its ligand in the **animal and wherein the antibody or antigen binding fragment thereof is capable of reducing said immune condition in an animal. 170h0ehdo0n oeo lisIt 6,weenteatbdyi l- rAt4 17. The method of any one of claims I to 16, wherein the antibody is antige orinding fragment thereof is selected from. a mnonoclonal antibody, a cbimeric antibody, a humanized antibody, a single chain variable fragment, a Fab fragment, a F(ab'), fragment and one or more CDRs. 19. A method for reducing an immune condition in an animal suffering from an immune condition or predisposed to suffer from the immune condition, wherein the immune condition is inflammation, substantially as herein described with reference to any one of the Examples. DATED this 28th day of July 2005 Sheiston 11? Attorneys for: ALTAREX MEDICAL CORP. aIAMr4 4 EqV2 COMS ID No: SBMI-01383081 Received by IP Australia: Time 10:41 Date 2005-07-28