AU759990B2 - Dithiolopyrrolones and their corresponding monoxides and dioxides as antineoplastic agents - Google Patents

Dithiolopyrrolones and their corresponding monoxides and dioxides as antineoplastic agents Download PDF

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AU759990B2
AU759990B2 AU90570/98A AU9057098A AU759990B2 AU 759990 B2 AU759990 B2 AU 759990B2 AU 90570/98 A AU90570/98 A AU 90570/98A AU 9057098 A AU9057098 A AU 9057098A AU 759990 B2 AU759990 B2 AU 759990B2
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hydrogen
aralkyl
cycloalkyl
aryl
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Genhui Chen
Jianxiong Li
John Malcolm Webster
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Welichem Biotech Inc
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    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07DHETEROCYCLIC COMPOUNDS
    • C07D495/00Heterocyclic compounds containing in the condensed system at least one hetero ring having sulfur atoms as the only ring hetero atoms
    • C07D495/02Heterocyclic compounds containing in the condensed system at least one hetero ring having sulfur atoms as the only ring hetero atoms in which the condensed system contains two hetero rings
    • C07D495/04Ortho-condensed systems
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    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00Medicinal preparations containing organic active ingredients
    • A61K31/33Heterocyclic compounds
    • A61K31/395Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/40Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with one nitrogen as the only ring hetero atom, e.g. sulpiride, succinimide, tolmetin, buflomedil
    • A61K31/407Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with one nitrogen as the only ring hetero atom, e.g. sulpiride, succinimide, tolmetin, buflomedil condensed with other heterocyclic ring systems, e.g. ketorolac, physostigmine
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00Antineoplastic agents

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Description

Replacement sheet 1 DITHILOPYRROLONES AND THEIR CORRESPONING MONOXIDES AND DIOXIDES AS ANTINEOPLASTIC AGENTS DESCRIPTION OF THE INVENTION FIELD OF THE INVENTION The present invention relates to dithiolopyrrolone derivatives, their corresponding monoxides (xenomins) and dioxides (xenorxides) having antineoplastic activities. The present invention also provides antineoplastic compositions comprising these compounds, the salts thereof, and methods of using the inventive compounds as antineoplastic agents.
SUMMARY OF THE INVENTION se The present invention provides dithiolopyrrolone derivatives, their corresponding monoxides (xenomins) and dioxides (xenorxides) having antineoplastic activities. The present invention also provides antineoplastic compositions comprising these compounds, the salts thereof, and methods of S using the inventive compounds as antineoplastic agents.
BRIEF DESCRIPTION OF THE DRAWINGS S. The following Figure 1, Figure 2 and Figure 3 represent the structural formula of dithiolopyrrolones, Stheir corresponding monoxides (xenomins) and dioxides (xenorxides), respectively.
R
1
S
S
R3 Figure 1 wherein Ri hydrogen, substituted or unsubstituted alkyl, cycloalkyl, aryl, aralkyl, or heterocyclyl group; R 2 hydrogen, substituted or unsubstituted alkyl, cycloalkyl, aryl, aralkyl, heterocyclyl or group acyl; R 3 hydrogen, alkyl, cycloalkyl, aralkyl, aryl or heterocyclyl group.
Replacement sheet 2 RI
R
I
0N
R
2 N -R S O r O S 2 N\
N
R3
R
3 Figure 2 wherein Ri= hydrogen, substituted or unsubstituted alkyl, cycloalkyl, aryl, aralkyl, or heterocyclyl group; R 2 hydrogen, substituted or unsubstituted alkyl, cycloalkyl, aryl, aralkyl, heterocyclyl or group acyl; R 3 hydrogen, alkyl, cycloalkyl, aralkyl, aryl or heterocyclyl group.
RI R1 O N -R 2 N -R 2 "O S Or 0 SO N N j 3 3 R 3 Figure 3 wherein R hydrogen, substituted or unsubstituted alkyl, cycloalkyl, aryl, aralkyl, or heterocyclyl group; R2 hydrogen, substituted or unsubstituted alkyl, cycloalkyl, aryl, aralkyl, heterocyclyl or group acyl; R 3 hydrogen, alkyl, cycloalkyl, aralkyl, aryl or heterocyclyl group.
BACKGROUND
Cancer is one of the major causes of death in humans and animals. Millions of people in the S world are diagnosed every year as having cancer, and a large proportion of these people subsequently die of cancer. Although large effort has been made, cancers remain to be hard-to-treat diseases. There continues to be an urgent need for effective anticancer drugs.
Soil organisms have been a traditional source ofbioactive materials for the Spharmaceutical and agrochemical industry. One of the recent developments has been the commercialization of a soil-living nematode-bacteria combination as biological control agents of insect pests. A crucial feature of this biocontrol agent is that the bacterial symbiont (Xenorhabdus spp. or Photorhabdus spp.) of the nematode produces a wide range of bioactive metabolites and some of these specific compounds have been isolated, identified and their structures elucidated (Forst and Nealson, 1996). Among these identified compounds, several are dithiolopyrrolone derivatives. Dithiolopyrrolones were initially WO 99/12543 PCT/CA98/00841 -3isolated from Streptomyces in the 40s and have since then been isolated from other organisms.
These compounds include aureothricin, thiolutin, holomycin and xenorhabdins (Hamao et al., 1948; Eisenman et al., 1953; Celmer and Solomons, 1955; von Daehne et al., 1969; McInerney et al., 1991). These compounds possess antimicrobial activity against a wide range of micro-organisms.
Recently, new antimicrobial substances, namely xenorxides (Webster et al., 1996), also were found from the bacterial cultures ofXenorhabdus spp. As a result of part of the ongoing exploration of the potential usefulness of these metabolites of Xenorhabdus, the xenorxides. xenomins and the dithiolopyrrolones have been found to be highly active against animal and human cancer cells, and is the subject of this invention.
Animal cells normally undergo various enzymatic and biochemical changes when influenced by various chemical factors. For example, when animal cells were challenged by carcinogens many enzymatic and biochemical activities can be promoted and some of the promoted activities can be experimentally detected. Among dithiolopyrrolones and their derivatives, thiolutin is the one that has been studied extensively. Thiolutin inhibits RNA polymerase synthesis (Jimenez et al.. 1973; Tipper, 1973) in yeast, has membrane stabilization activity and inhibit platelet aggregation (Yasuyuki et al., 1980) in rat. It has been reported (Menta and Moon, 1991; Sharma et al., 1994; Arnold et al., 1995) that thiolutin inhibited the promotion of several such activities that are possibly related to carcinogenesis in mammary cells that have been exposed to carcinogens.
This suggested that thiolutin might be involved in the initiation of cell transformation or the progression of premalignant cells becoming malignant cancer cells. However, this activity has not experimentally established. Although the study of dithiolopyrrolones has been on-going for about years. the anticancer activity of thiolutin and other dithiolopyrrolones against mammary cells has never been evaluated and reported until now. Xenorxides and xenomins have been discovered very recently, and there is no report in the prior art on their anticancer activity.
DETAILED DESCRIPTION OF THE INVENTION Although the compounds of the present invention may be synthesized chemically, the initial compounds were obtained from micro-organisms. The organisms used for this invention include Xenorhabdus bovienii, a symbiotic bacterium associated with entomopathogenic nematodes. X bovienii and its nematode symbiont Steinernemafeltiae, used in this invention, were collected from soil in British Columbia, Canada. Briefly, last instar larvae of the Greater Wax Moth, Galleria mellonella, were infected with infective juvenile (IJ) nematodes, carrying the X bovienii A21 strain, at a rate of 25 IJs/larvae. After 24 to 48 h the dead insect larvae were surface disinfected by dipping them into 95% EtOH and igniting them. The cadavers were aseptically dissected, haemolymph was streaked onto an SUBSTITUTE SHEET (RULE 26) WO 99/12543 PCT/CA98/00841 -4agar culture medium and incubated in the dark at room temperature. The agar medium has the following composition in one litre of distilled water: beef extract 3 g peptone 5 g bromothymol blue 0.025 g 2,3,5-triphenyltetrazolium 0.04 g Agar 15 g Sterilized at 121 OC for 15 minutes.
The resulting primary form ofX. bovienii was maintained and subcultured at 14 d intervals.
For consistency, 14% sucrose lyophilized powder of the bacteria stored at -20 0 C was frequently used as the starting material for cultures. Cultures ofX. bovienii A21 strain from which the inventive compounds are obtained exhibit the characteristics listed in Table 1 and Table 2: Table 1. Biochemical properties of Xenorhabdus bovienii A21 strain.
Gram reaction Cell size (rim) Mobility Cell peritrichous Pigmentation Catalase Oxidase Urease 5.3x2.2 yellow Lecithinase Lipase(Tween 80) positive; negative.
Table 2. Acid production and utilization of carbon sources by Xenorhabdus bovienii A21 strain.
Acid production* Utilization of carbon sources D-Arabinose Asparagine Esculine Cystine D-Fructose Glysine D-Galactose Tyrosine D-Glucose Nicotinic acid SUBSTITUTE SHEET (RULE 26) WO 99/12543 PCT/CA98/00841 Inositol Ethanol Inulin Methanol D-Lactose Inositol D-Maltose Mannose D-Mannitol D-Galatose D-Mannose D-Glucose D-Raffinose D-Lactose D-Sorbitol D-Mannitol L-Sorbose D-Sorbitol D-xvlose Ribose positive; weakly positive; negative.
These characteristics are in agreement with those described for X bovienii by Akhurst, R. J.
and N. E. Boemare (1988), and, therefore, establishes the identity of the organism as X bovienii.
The bacterial strain from which the compounds of this invention were isolated was deposited under the Budapest Treaty in the American Type Culture Collection (ATCC), Rochville, MD with a deposition number of ATCC 55743.
Cultivation of the microorganism X bovienii yields bioactive substances, xenomins.
xenorxides and dithiolopyrrolones. X bovienii may be cultivated (fermented), for example, at about °C under submerged aerobic conditions in an aqueous, nutrient medium containing assimilable carbon (carbohydrate) and nitrogen sources to obtain xenomins, xenorxides and dithiolopyrrolones.
The fermentation may be carried out for a time period such as approximately 48 to 96 hours, at the end of which time these compounds have been formed, and may be isolated from the fermentation medium and purified.
After the fermentation has been completed, the fermented broth may be filtered or centrifuged and the pH of the filtrate adjusted to about 7.0 by the addition of hydrochloric acid or kept as it is. The filtrate may then be extracted with a water immiscible, organic solvent, for example, with ethyl acetate or chloroform. The combined organic layers pooled ethyl acetate or chloroform extracts) may be concentrated under vacuum at about 30 °C to an oily residue.
The oily residue may be mixed with a small amount of organic solvent and chromatographed on a silica gel column. After introduction of the sample, chloroform or other organic solvent may be applied to elute the bioactive fraction. The bioactive fraction may be purified further by high performance, liquid chromatography (HPLC) with organic and/or aqueous solution.
SUBSTITUTE SHEET (RULE 26) WO 99/12543 PCT/CA98/00841 -6- In a conventional culture, the major compounds produced by X. bovienii are dithiolopyrrolones while xenorxides and xenomins are present in relatively small amounts.
Alternatively, dithiolopyrrolones may be produced from other micro-organisms, by chemical method(s) and/or a combination of both approaches.
The monoxides and dioxides of the corresponding dithiolopyrrolones isolated from X bovienii, namely, xenorxides and xenomins, respectively may be transformed from their corresponding dithiolopyrrolone derivatives by biological or chemical means. By biological means, the culture broth ofX bovienii, with the corresponding dithiolopyrrolone derivatives present, may be filtered or centrifuged. The cell-free filtrate may be open to the air for extended periods from one week up to one month with or without stirring at room temperature or at other temperatures.
This process may oxidize all or part of the corresponding dithiolopyrrolone derivatives to xenomins and xenorxides. Xenorxides and xenomins may be obtained by oxidation of the corresponding dithiolopyrrolone derivatives by chemical means. The pure dithiolopyrrolone derivatives or their mixture may be dissolved in a mixture of acetone and water, then oxidizing reagents such as potassium peroxymonosulfate and potassium bicarbonate may be added to the mixture. The mixture may be allowed to react for a period of several minutes up to more than one hour. The reaction mixture may be mixed with water, and extracted with organic solvent. The extracts may be combined, dried and purified by column chromatography or HPLC to obtain the corresponding xenorxides and/or xenomins. Additional monoxides and dioxides of the dithiolopyrrolones may be obtained from different microorganisms, synthesized chemically and/or produced by a combination of biolological and chemical methods, and several examples are disclosed (Takahashi, et al., 1995; Fujimoto, et al. 1996).
The compounds of the present invention include dithiolopyrrolones, their corresponding monoxides such as xenomins, their corresponding dioxides such as xenorxides, and salts thereof.
The term "salts", as used herein, denotes acidic and/or basic salts, formed with inorganic and/or organic acids and bases. Suitable acids include, for example, hydrochloric, sulfuric, nitric, benzenesulfonic, acetic, maleic, tartaric and the like which are pharmaceutically acceptable. It is well known to one skilled in the art that an appropriate salt is chosen based on physical and chemical stability, flowability, hydroscopicity and solubility. While pharmaceutically acceptable salts are preferred, particularly when employing the compounds of the invention as medicaments, other salts find utility, for example, in processing these compounds, or where non-medicamenttype uses are contemplated.
The antineoplastic agents and use thereof.
The present invention relates to pharmaceutical preparations which contain an active ingredient of these compounds or a pharmaceutically acceptable salt thereof. The dosage form and mode of SUBSTITUTE SHEET (RULE 26) WO 99/12543 PCT/CA98/00841 administration, as well as the dosage amount, may be selected by the skilled artisan. Exemplary daily dosages for an adult human are those within the range of about 2.5 mg to about 2,000 mg/day. Administration to a mammalian host may, for example, be oral, parenteral. or topical.
When these compounds or the salts thereof are used as therapeutics, they can be administrated alone or in a pharmaceutically suitable formulation containing, in addition to the active ingredient, one or more conventional carriers. Depending on the nature of the disease and/or route of administration, the composition of this invention can be formulated by known means.
Examples of pharmaceutical compositions include any solid (tablets, pills, capsules, granules, powder etc.) or liquid (solutions, suspensions or emulsions) compositions suitable for oral, topical or parenteral administration, and they may contain the pure compound or a salt thereof or in combination with any carrier or other pharmaceutically active compounds. These compositions may need to be sterile when administered parenterally.
The therapeutic compositions of the present invention being employed as antineoplastic agents for treatment of animal and human illness can be easily prepared in such unit dosage form with the employment of pharmaceutical materials which themselves are available in the art and can be prepared by established procedures. The appropriate solid or liquid vehicle or diluent may be selected, and the compositions prepared, by methods known to the skilled artisan. The administration of any of the inventive compound and/or its pharmacologically active and physiologically compatible derivatives is useful for treating animals or humans having a neoplastic disease, for example, colon cancer, cevercal cancer, breast cancer, leukemia, lung cancer. ovarian cancer. CNS cancer. renal cancer, prostate cancer, etc. using the accepted protocols of the National Cancer Institute (NCI). The dosage administered will be dependent upon the identity of the neoplastic disease. the type of host involved including its age, health and weight, the kind of concurrent treatment, if any, and the frequency of treatment and therapeutic ratio. Illustratively, dosage levels of the administered active ingredients are intravenous, 0. 1 to about 200 mg/kg; intramuscular, 1 to about 500 mg/kg and orally, 5 to about 1000 mg/kg of host body weight.
Expressed in terms of concentration, an active ingredient can be present in the compositions of the present invention for localized use in a concentration from about 0.01 to about 50% w/w of the composition, preferably about 1 to about 20% w/w of the composition, and for parenteral use in a concentration of from about 0. 05 to about 50% w/v of the composition and preferably from about to about 20% w/v. The active ingredients to be employed as antineoplastic agents, can be easily prepared in such unit dosage form with the employment of pharmaceutical materials which themselves are available in the art and can be prepared by established procedures.
SUBSTITUTE SHEET (RULE 26) WO 99/12543 PCT/CA98/00841 -8- EXAMPLE 1. preparation of xenorxides, xenomins and the dithiolopyrrolones A. Preparation of selected dithiolopyrrolones.
Several dithiolopyrrolones have been reported so far, these include aureothricin, thiolutin, holomycin, xenorhabdins and thiomarinol. Aureothricin was initially reported by Umezawa et al.
(1948), the structure were fully disclosed by Celmer and Solomons (1955). Thiolutin is produced by several bacterial species including Streptoverticillium album, which is available from the American Type Culture Collection(ATCC), Rockville, MD with an ATCC number of 33049. The production of thiolutin and holomycin by chemical synthesis was published by Schmidt and Geiger (1962), Hagio, K. and Yoneda, N. (1974) and Stachel, et al. (1992). Production of xenorhabdins was reported and fully reported by Mclnerney et al. (1991) and by Li et al.(1995).
The preparation of additional different compounds with the dithiolopyrrolone ring was recently disclosed by Baggaley et al. (1994a, b) and by Takahashi et al (1994). The dithiolopyrrolones used for the subject invention are prepared by the methods described in the cited references, and the structure of each dithiolopyrrolone derivative confirmed by its NMR spectroscopy. Skilled chemists will be able to use procedures as disclosed herein, and others, to obtain these dithiolopyrrolones from available stock substances. In carrying out such operations, any suitable filtration, chromatographic, and other purification techniques might be employed by those skilled in the art. It will be apparent to those skilled in the art that materials and reagents to carry out such operations are commercially available from chemical companies, so no details are given respecting them.
B. Preparation of the corresponding dioxides from dithiolopyrrolones by microbial fermentation.
Xenorxides: Cultures ofX bovienii were shaken at 180 rpm on an Eberbach gyrorotary shaker for 24 h at 25 °C Bacterial fermentation was initiated by adding 100 ml of this bacterial culture to 900 ml of tryptic soy broth in a 2,000 ml flask. The flask was incubated in the dark at °C on a gyrorotary shaker. After 96 h, the culture was immediately centrifuged (12,000 g, minutes, 4 °C to separate the bacterial cells. The cell-free broth was then extracted with ethyl acetate four times. The combined extracts were dried with anhydrous sodium sulfate and then filtered through filter paper. The filtrate was concentrated on a rotary evaporator below 30 OC under vacuum to yield a brown oil. After the above experiment was repeated 10 times, approximately 3 g of the oil was obtained. The crude extracts were then loaded onto a silica gel (200 g silica gel 60, 40 cm x 5 cm, EM Science, Darmstadt, Germany) chromatographic column.
The yellow bioactive fraction was eluted out with ether or ethyl acetate. This bioactive fraction was then subject to HPLC on a C 1 8 preparative column (Spherisorb 10 250 SUBSTITUTE SHEET (RULE 26) WO 99/12543 PCT/CA98/00841 -9- X 10 mm, 10 micro, Phenomenex, Torrance, CA) with a program (isocratic at 10% acetonitrile in water for 5 min, then gradually increasing to 85% acetonitrile in 35 min, isocratic for 5 min, then decreasing back to 10% in 2 min) at 2.5 ml/min. The eluate was monitored at 254 nm.
XENORXIDE 1 (about 0.3 mg per liter of the culture broth) was eluted at 33.6 min, and this was followed by XENORXIDE 2 (0.2 mg/1) which was eluted at 35.2 min.
C. Preparation of the corresponding monoxides from dithiolopyrrolones by microbial fermentation.
Xenomins: For the purification of xenomins, the culture and extraction conditions were the same as for xenorxides. After the extraction, the concentrated crude extract was processed through a silica gel chromatographic column with ethyl acetate as the eluent. After the less polar bioactive material was eluted, the more polar bioactive fraction was obtained by eluting with methanol. The more polar bioactive fraction was concentrated under vacuum, and separated by a Ci 8 chromatographic column first with water as eluent, then 25% methanol in water, 50% methanol, methanol and finally, pure methanol. The most bioactive fraction was eluated with methanol in water. This bioactive fraction was then concentrated and separated by HPLC on a C18 preparative column (Spherisorb 10 250 x 10 mm, 10 micro, Phenomenex, Torrance, CA) with a program (30% MeCN in H 2 0 for 1 min and gradually increasing to 70% MeCN in H 2 0 in 24 min, isocratic for 5 min) at 2.0 ml/min. The eluate was monitored at 254 nm. Active peak 1 (25.4 min) and peak 2 (25.8 min) were collected. Active peak 1 was concentrated, and further separated by preparative silica gel TLC with 60% ethyl acetate in dichloromethane as the eluent to give xenomin 1 (R 0.32). Active peak 2 was concentrated, and further separated by preparative silica gel TLC with 60% ethyl acetate in dichloromethane as the eluent to give xenomin 2 (Rf= 0.31).
D. Preparation of xenorxides from their corresponding dithiolopyrrolone derivatives by biological transformation.
The cell-free broth was obtained using the same method as described above, and was then stored at 4 OC to room temperature for 3 to 6 weeks. Then the aqueous broth was extracted with ethyl acetate, and the combined extracts were separated using the same process discussed above.
XENORXIDE 1 was eluted at 33.6 min (2 mg/1), XENORXIDE 2 was eluted at 35.2 min mg/1).
E. Preparation of the corresponding dioxides from dithiolopyrrolones by chemical oxidation.
One hundred and fifty two mg of 6-(hexanamido)-4-methyl-4,5-dihydro-1.2-dithiolo[4,3-b] (XN1) were dissolved in a mixture of 20 ml of acetone and 15 ml of water, and then cooled to 0 °C with ice. Potassium peroxymonosulfate (510 mg) was added to the solution at 0 °C.
The reaction mixture was stirred for one hour at 0 OC. Then 5 ml of saturated solution of potassium bicarbonate was added to the reaction mixture with continuous stirring for 0.5 h at 0°C. The reaction SUBSTITUTE SHEET (RULE 26) WO 99/12543 PCT/CA98/00841 mixture. after water was added, was extracted with ethyl acetate three times. The extracts were combined, dried over Na 2
SO
4 and evaporated to give the crude product, which was then purified by silica gel column chromatography with hexane: ethyl acetate to give pure xenorxide 1 (78 mg, 51% yield). Similarly, xenorxide 3 was produced from 6-(5'-methylhexanamido)-4,5-dihydro-1,2dithiolo[4,3-b] pyrrol-5one with 50% yield.
Example 2. Identification of the active components NMR spectra were recorded on a Bruker WM400 spectrometer in CDCl 3 using residual CDC13 as internal standard. Low resolution mass spectra were obtained on a Hewlett- Packard 5985B GC/MS system operating at 70 eV using a direct probe. High resolution MS spectra were recorded on a Kratos MS80 instrument. IR spectra were recorded as neat film on NaCl using a Perkin-Elmer 599B spectrometer. (Abbreviations used as follows: El Electron Impact, M Molecular Ion. t triplet, J coupling constant, Hz Hertz, d doublet, m multiplet, bs broad singlet).
6-(Acetamido)-4-methyl-4,5-dihydro- 1.2-dithiolo[4,3-b] pyrrol-5-one (Thiolutin) (XNO): 'HNMR (DMSO-d 6 6 2.05 (3H, 3.92 (3H, 7.19 (1H, s) and 9.85 (1H, broad EIMS m/e 228 186.
6-(Hexanamido)-4-methyl-4,5-dihydro-1.2-dithiolo[4,3-b] pyrrol-5-one (XN1): 'HNMR
(CDCI
3 8 0.90 (3H, t, J=6.9Hz), 1.35 (4H, 1.70 (2H, 2.35 (2H, t, J=7.4Hz), 3.35 (3H, s), 6.63 (1H, s) and 7.43 (1H, broad EIMS m/e 284 (M 186.
6-(5'-Methylhexanamido)-4,5-dihydro-1,2-dithiolo[4,3-b] pyrrol-5one (XN3): 'HNMR (CDC13) 8 0.89 (6H, d, J=6.6Hz), 1.24 (3H, 1.70 (2H, 2.32 (2H, t, J=7.6Hz), 6.74 (1H, s), 7.44 (1H. broad s) and 7.94 (1H, broad EIMS m/e 284 172.
XENORXIDE 1 (X01): EIMS: 317(2), 316(M+, 13), 220(9), 219(9), 218(100), 186(23), 154(16), 99(40). 71(39); HRMS: 316.0555 (Calc. for C12H16N204S2: 316.0551, 20), 217.9824 (Calc. for C6H6N203S2: 217.9820, 100), 154.0197 (Calc. for C6H6N2OS: 154.0201, 16); IR (KBr): 3448, 3298. 3275, 1720, 1686, 1654, 1637, 1560, 1522, 1310, 1139, 551 cm- 1
IHNMR
(CDC13) 8: 7.56 (1H, bs, CO-NH), 6.35 (1H, s, 3.20 (3H, s, N-Me), 2.38 (2H, t, CO-CH2, J=7.4 Hz), 1.67 (2H, m, CH2), 1.32 (4H, m, CH2CH2), 0.89 (3H, t, J=7.0 Hz); 13CNMR (CDC13) 171.6(s, CON), 164.7 CO), 145.4(s, C7), 121.3(s, C6), 116.2(s, C8), 109.2(d, C3), 36.4, 31.2, 27.8, 24.6, 22.3, 13.8.
XENORXIDE 2 (X02): EIMS: 330(M 10), 218(100); HRMS: 330.0707 (Calc. for C13H18N204S2: 330.0708, 18), 217.9829 (Calc. for C6H6N203S2: 217.9820, 100), 154.0213 (Calc. for C6H6N20S: 154.0201, 16); IR (KBr): 3438, 3298, 1719, 1686, 1654, 1637, 1560, 1522, 1400.
SUBSTITUTE SHEET (RULE 26) WO 99/12543 PCT/CA98/00841 -11- 1310, 1142, 551 cm-1; IHNMR (CDC13) 8: 7.56 (1H, bs,CO-NH), 6.35 (1H, s, 3.20 (3H, s, N-Me), 2.36 (2H, t, CO-CH 2 J=7.4 Hz), 1.67 (2H, m, CH 2 1.2-1.6 (1H, m, CH), 1.22 (2H, m, CH2), 0.89 (6H, d, J=6.6Hz); Different NOE experiment showed the NOE effect between the peak at 6.35 ppm and 3.20 ppm; 1 3 CNMR (CDC13) 8: 171.6(s, CON), 164.7 CO), 145.4(s, C7), 121.3(s, C6), 116.2(s, C8), 109.2(d, C3), 38.2(t, CH2), 36.7(t, CH2), 28.0(q, CH3), 27.8(d, CH), 22.8(t, CH2), 22.4(q, CH3).
XENORXIDE 3 (X03): EIMS: 316(M+, 218(9), 204(65) 185(7), 172(15), 105 95(95), 69(100), 43 1HNMR (CDC13) 8: 10.10 (1H, bs), 9.32 (1H, bs), 6.85 (IH, s, 2.53 (2H, t, CO-CH2, J=7.5 Hz), 1.66 (2H, m, CH2), 1.58 (1H, 1.21 (2H, m, 0.87 (6H, d, J=6.6 Hz).
Xenomin 1(XMI): EIMS: 300(M 13), 202(36), 186(44), 185(41), 85(62), 69(100); HRMS: 300.0605 (Calc. for CI 2 H16N 2 0 3
S
2 300.0602. 12), 201.9871 (Calc. for C6H 6
N
2 0 2
S
2 201.9871, 30); 'HNMR (CDC1 3 d: 7.52 (1H, bs.CO-NH), 6.46 s, 3.30 (3H, s. N-Me), 2.49 (2H. t, CO-CH2, J=7.4 Hz), 1.79 (2H. m. CH2), 1.41 (4H. m, CH 2
-CH
2 0.90 (3H, t, J=6.9).
Xenomin 2 (XM2): EIMS: 314(M 21), 218(15), 202(59), 187(18), 186(98), 185(73), 69(100); HRMS: 314.0759 (Calc. for C1 3
H
18
N
2 0 3
S
2 314.0759, 12), 201.9871 (Calc. for
C
6
H
6 N20 2
S
2 201.9871, 45); 'HNMR (CDC13) d: 7.43 (1H, bs,CO-NH), 6.46 s, 3.30 (3H, s, N-Me), 2.48 (2H, t, CO-CH 2 J=7.6 Hz), 1.71 (2H, m, CH2), 1.29 (3H, m, CH and CH 2 0.88 (6H, d, Example 3. Dithiolopyrrolones, the corresponding monoxides and dioxides as antineoplastic agents.
The anticancer activity of a particular compound can be demonstrated by standard assays.
The method normally used by the American National Cancer Institute (NCI) for the effectiveness of a compound is based on the LCso. The LCso is the compound concentration at which half of the cancer cell population is killed. The assay is commonly used by those skilled in the art and are accepted as indicative of anticancer activity in mammals. The test animal cancer cells are available from ATCC, NCI and other organizations. The anticancer activities of compounds of the present invention have been determined in cell cultures of various human cancer cells (see table below) using the standard NCI method with slight modifications. The specifications of the procedure was described by Skehan et al. (1990). Briefly, cancer cells were grown in RPMI-1640 medium with glutamine and 10% fetal calf serum, and were harvested from exponential-phase maintenance cultures. The harvested cells were counted and dispensed into replicate 96-well culture plates in 180 pl volumes for each well with a cell density of up to 2,500 cells/well. The cells were allowed to settle for about 4 hours at 37 Then 20 ul of medium containing the test compound was added into each well. resulting in a suitable final test compound concentration. The test plates were then incubated at 37 OC. The test was terminated after SUBSTITUTE SHEET (RULE 26) WO 99/12543 PCT/CA98/00841 -12incubation by adding to each well 50 pl of cold 50% trichloroacetic acid. The cells were fixed for an hour at 4 °C and then washed five times with tap water. The washed plates were air-dried and stained for 30 minutes with 0.4% (wt/vol) sulforhodamine B (SRB) which was dissolved in 1% acetic acid. At the end of the staining period, the SRB was removed and plates were quickly rinsed five times with 1% acetic acid. After being rinsed, the plates were air-dried and 100 gl of 10 mM tris base (pH 10.5) were added to each well to dissolve the dye bound to the cells. The plates were placed on a gyratory shaker and shaken (100 rpm) for 10 minutes. Finally, the plates were read in a microtiter plate reader at 570 rnm. All the compounds tested exhibited very strong anticancer activity against these cancer cells (see table below).
Antitncoplastic activity:
LC
5 010" NIM I Lanccr cells XN X\ X N3 XNO.)I NX02 XM1 Xi2 HT29 0.13 0.11 0.11 0.22 0.85 0.47 0.11 MCF-7 0.35 0.56 0.39 0.89 0.95 0.47 0.55 Hela 0.31 0.46 0.32 0.16 0. 13 0.80 0.21 P388 0.66 0.28 NC1-H46(0_ 0.03 0.28 0.22 Sk-Mel-28- 0.24 0.1) 0.73 LNCap 0.54 0.19 DlI'-145 (Prostate 0.14 1.80 1.60 1.0-31 (Renal cancer) 0.18 1.85 6.0 SF-295 (CNS cancer) 0.04 1.58 2.12 not tested.
From the foregoing embodiments and examples, it is apparent that an invention has been herein described and illustrated. While our above description contains many specificities, these should not be construed as limitations on the scope of the invention, but rather as examples of preferred embodiments. Accordingly, the scope of the invention should not be determined by the embodiments presented, but by the appended claims and their legal equivalents.
SUBSTITUTE SHEET (RULE 26) WO 99/12543 WO 9912543PCT/CA98/0084
I
-13- REFERENCES CITED 1. Akhurst, R. J. and N. E. Boemare, 1988. J. Gen. Microbiol. Vol. 134, 1835-1845 2. Arnold, J. T. et al., 1995. Cancer Research 55:537-543.
3. Baggaley, K.H. et al., 1994a.WO 94/26750.
4. Baggaley, K.H. ei al., 1994b. WO 94/28001.
Celmer, W. D. and 1. A. Solomons 1955. Amer. Chem. Soc. 77:2861-2865.
6. Eisenrnan, W. ei' al., 1953. Antibiotics and Chemotherapy 3: 385-392.
7. Forst, S. and K. Nealson, 1996. Microbiol. Rev. 60:21-43.
8. Fujimoto, K. et al.. 1996. WO 96/23795.
9. Hagia, K. and Yoneda, N. 1974. Bull. Chem. Soc. Japan. 47:1484-1489.
Jimenez. A. et al.. 1973 Anuirnicrob. Chernother. 729-73 8.
11. Li. et al., 1995.-App!. Environ. Microbiol. 61:43 29-4333.
12. Li. J. et al., 1995.1. Nat. Prod. 58:1081-1085.
13. Mclnerney, B. V. et al., 1991.1. Vat. Prod 54:774-784.
14. Menta R. G. and R. C. Moon, 1991. Anticancer Research 11:593-596.
Monks et al., 1991.1. Natl. Cancer Inst. 83:757-766.
16. Ninomiya, Y. T. et al., 1980. Chem. Pharm. Bull. 28:3157-3162.
17. Sharma, S. et al.: 1994. Cancer Research 54:5 848-5855.
18. Shroeder et al.. 1981. MAed Chem. 24:1078-1083.
19. Skehan, P. et al.. 1990. J. Natl. Cancer Inst 82:1107-1118.
Stachel, H.D. et 1992. Liebigs Ann. Chemn. 473-480.
21. Takahashi S. et 1994. US patent. 5,292,892.
22. Takahashi S. et 1995. US patent. 5,399,711.
23. Tipper D. J. 1973 Bacteriol. 116:245-256.
24. Umezawa, H. et a. 1948. Jap. Med. 1. 1:512-517.
Webster et al. WO: 96/32396.
26. von Daehne, et al., 1969. 1. Antibiotics 22:233-235.
SUBSTITUTE SHEET (RULE 26)

Claims (12)

1. A pharmaceutical composition for treating neoplastic diseases comprising a compound having the following structure formula R1 R 1 0 N-R 2 N-R 2 S O Or O N N •R o, 3 R 3 wherein R 1 hydrogen, substituted or unsubstituted alkyl, cycloalkyl, aryl, aralkyl, or heterocyclyl group; R 2 hydrogen, substituted or unsubstituted alkyl, cycloalkyl, aryl, aralkyl, or heterocyclyl, acyl group; R 3 hydrogen, alkyl, cycloalkyl, aralkyl, aryl or heterocyclyl group, or a pharmaceutically acceptable salt thereof, in association with a pharmaceutical carrier or dilution. 0
2. A pharmaceutical composition for treating neoplastic diseases comprising a compound of S claim 1, wherein R, hydrogen; R 2 acyl; R 3 hydrogen, methyl; or a pharmaceutically acceptable salt thereof, in association with a pharmaceutical carrier or dilution. S
3. A pharmaceutical composition for treating neoplastic diseases comprising a compound of claim 1, wherein R 1 hydrogen, R 2 acyl group with a straight or branched one to ten carbon chain; R 3 hydrogen, or methyl, or a pharmaceutically acceptable salt thereof in association with a pharmaceutical carrier or dilution.
4. A method of inhibiting the growth of mammalian tumors, comprising administrating to a Soo* 50 subject in need of such treatment, an effective antineoplastic amount of a compound of the structures shown below, Replacement sheet RI N -R 2 S S O N R3 wherein R 1 hydrogen, substituted or unsubstituted alkyl, cycloalkyl, aryl, aralkyl, or heterocyclyl group; R 2 hydrogen, substituted or unsubstituted alkyl, cycloalkyl, aryl, aralkyl, or heterocyclyl, acyl group; R 3 hydrogen, alkyl, cycloalkyl, aralkyl, aryl or S heterocyclyl group, or a pharmaceutically acceptable salt thereof, in association with a pharmaceutical carrier or dilution.
A method of inhibiting the growth of mammalian tumors, comprising administrating to a subject in need of such treatment, an effective antineoplastic amount of a compound of the Sstructures shown below, 00 R R @0 SN-R 2 N -R 2 r 0 S N N SR3 R 3 wherein RI= hydrogen, substituted or unsubstituted alkyl, cycloalkyl, aryl, aralkyl, or heterocyclyl group; R 2 hydrogen, substituted or unsubstituted alkyl, cycloalkyl, aryl, aralkyl, or heterocyclyl, acyl group; R 3 hydrogen, alkyl, cycloalkyl, aralkyl, aryl or S heterocyclyl group, or a pharmaceutically acceptable salt thereof, in association with a pharmaceutical carrier or dilution.
6. A method of inhibiting the growth of mammalian tumors, comprising administrating to a subject in need of such treatment, an effective antineoplastic amount of a compound of the structures shown below, Replacement sheet -16- ^Or l N R3 R3 wherein RI= hydrogen, substituted or unsubstituted alkyl, cycloalkyl, aryl, aralkyl, or heterocyclyl group; R2= hydrogen, substituted or unsubstituted alkyl, cycloalkyl, aryl, aralkyl, or heterocyclyl, acyl group; R 3 hydrogen, alkyl, cycloalkyl, aralkyl, aryl or heterocyclyl group, or a pharmaceutically acceptable salt thereof, in association with a Spharmaceutical carrier or dilution.
7. A method of inhibiting the growth of mammalian tumors, comprising administrating to a Ssubject in need of such treatment, an effective antineoplastic amount of a compound of claim 4, wherein R 1 hydrogen; R 2 acyl; R 3 hydrogen, methyl; or a pharmaceutically acceptable salt thereof, in association with a pharmaceutical carrier or dilution.
8. A method of inhibiting the growth of mammalian tumors, comprising administrating to a subject in need of such treatment, an effective antineoplastic amount of a compound of claim 5, wherein Ri hydrogen; R 2 acyl; R 3 hydrogen, methyl; or a pharmaceutically acceptable salt thereof, in association with a pharmaceutical carrier or dilution.
9. A method of inhibiting the growth of mammalian tumors, comprising administrating to a subject in need of such treatment, an effective antineoplastic amount of a compound of claim 6, wherein Ri hydrogen; R 2 acyl; R 3 hydrogen, methyl; or a pharmaceutically S* acceptable salt thereof, in association with a pharmaceutical carrier or dilution. S•
10. A method of inhibiting the growth of mammalian tumors, comprising administrating to a subject in need of such treatment, an effective antineoplastic amount of a compound of claim 4, wherein RI= hydrogen, R 2 acyl group with a straight or branched one to ten carbon chain; R 3 hydrogen, or methyl, or a pharmaceutically acceptable salt thereof in association with a pharmaceutical carrier or dilution.
11. A method of inhibiting the growth of mammalian tumors, comprising administrating to a IZ, bject in need of such treatment, an effective antineoplastic amount of a compound of claim Replacement sheet -17- wherein R 1 hydrogen, R 2 acyl group with a straight or branched one to ten carbon chain; R 3 hydrogen, or methyl, or a pharmaceutically acceptable salt thereof in association with a pharmaceutical carrier or dilution.
12. A method of inhibiting the growth of mammalian tumors, comprising administrating to a subject in need of such treatment, an effective antineoplastic amount of a compound of claim 6, wherein Ri= hydrogen, R 2 acyl group with a straight or branched one to ten carbon chain; R 3 hydrogen, or methyl, or a pharmaceutically acceptable salt thereof in association with a pharmaceutical carrier or dilution. 0 06 S S 00
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