AU759274B2 - 2-(4-(4-(4,5-dichloro-2-methylimidazol-1-YL) butyl)-1-piperazinyl)-5-fluoropyrimidine, its preparation and its therapeutic use - Google Patents

Description

AUSTRALIA

Patents Act COMPLETE SPECIFICATION

(ORIGINAL)

Class Int. Class Application Number: Lodged: Complete Specification Lodged: Accepted: Published: Priority IP Australia

E

CD

Documents received on: 0 1 M1 AY 2001 Batch No: Related Art: Name of Applicant: Laboratorios Del Dr. Esteve, S.A.

Actual Inventor(s): Ramon Merce-Vidal, Jordi Frigola-Costanza Address for Service: PHILLIPS ORMONDE FITZPATRICK Patent and Trade Mark Attorneys 367 Collins Street Melbourne 3000 AUSTRALIA Invention Title: 2-{4-[4-(4,5-DICHLORO-2-METHYLIMIDAZOL-1-YL)BUTYL]-1-PIPERAZINYL}-5- FLUOROPYRIMIDINE, ITS PREPARATION AND ITS THERAPEUTIC USE Our Ref: 643137 POF Code: 2975/113338 The following statement is a full description of this invention, including the best method of performing it known to applicant(s): -1- -l.

2-{4-[4-(4,5-DICHLORO-2-METHYLIMIDAZOL-1 -YL)BUTYL]-1 -PIPERAZINYL}- ITS PREPARATION AND ITS THERAPEUTIC USE This application is a divisional of Australian patent application 79170/98 (732722) the entire content of which is herein incorporated by reference.

The present invention relates to the novel compound 2-{4-[4-(4,5-dichloro- 2-methylimidazol-1-yl)butyl]-1-piperazinyl}-5-fluoropyrimidine, its preparation and its therapeutic use, its physiologically acceptable salts, the processes for their preparation and their application as medicaments in human and/or veterinary therapeutics, and the pharmaceutical compositions which comprise them.

Cl Cl N kN N N- N F C Y I

N

CH

3 Patents EP 392,637 and EP 497,659 of the Applicant Company disclosed various pyrimidinylpiperazinylalkylazole derivatives having anxiolytic and/or tranquilizing properties. Although Patent EP 382,637 claims pyrimidinylpiperazinylalkylazole pyrimidine by a halogen atom, only two examples of compounds of this type are disclosed and, in both cases, it is a bromine atom.

The above discussion of documents, acts, materials, devices, articles and the like is included in this specification solely for the purpose of providing a context for the present invention. It is not suggested or represented that any or all of these matters formed part of the prior art base or were common general knowledge in the field relevant to the present invention as it existed in Australia 0 before the priority date of each claim of this application.

Throughout the description and claims of the specification the word "comprise" and variations of the word, such as "comprising" and "comprises", is not intended to exclude other additives, components, integers or steps.

The Applicant Company has now discovered that the introduction of a fluorine atom as substituent at the 5-position of the pyrimidine, in the special case where the azole is an imidazole trisubstituted by a methyl group at the 2-position and by two chlorine atoms at the 4- and 5- positions, gives rise to the compound which is the subject-matter of the present patent, which compound exhibits some advantageous biological properties which make it of particular use Y:\fiona\NKI\Spccics\79170 doc in its application in human and/or veterinary therapeutics. In particular, the compound which is the subject-matter of the present patent is of use as an antiemetic against seasickness (nausea caused by motion), as an antidepressant or anxiolytic, as an inhibitor of gastric acid secretion or obsessive-compulsive disorders, in panic attacks and in sleep apnea in mammals, including man. In one aspect the present invention provides a method of treatment emesis comprising administration of a therapeutically effective amount of the compound 2-{4-[4,5-dichloro-2-methyl-imidazol-1-yl)butyl]-1and its physiologically acceptable salts of formula CI Cl

N

N N \N N F 'Y

N

CH

3 or of the compound 2-{4-[4-(4,5-dichloro-2-methyl-imidazole-1-yl)butyl]piperazinecitrate.

;i It is possible to prepare the compound 2-{4-[4-(4,5-dichloro-2-methylimidazol-1yl)butyl]-1-piperazinyl]-5-fluoropyrimidine and its physiologically acceptable salts according to the invention by one of the processes shown hereinbelow.

*15 a) by reaction of 8-(5-fluoro-2-pyrimidinyl)-8-aza-5-azoniaspiro[4.5] decane with 4,5-dichloro-2-methyl-1H-imidazole in the presence of potassium carbonate and in a dipolar aprotic solvent, such as dimethylformamide. The reaction temperature can vary between 70°C and the reflux temperature of the solvent, and the reaction time fluctuates 20 between 3 and 48 hours.

Cl Cl C**l C N i **Br HN

C

CH

3 b) by reaction of 5-fluoro-2-(piperazine-1-yl)-pyrimidine with 1-(4-chlorobutyl)- 4,5-dichloro-2-methyl-1H-imidazole in the presence of potassium carbonate and in a dipolar aprotic solvent, such as dimethylformamide. The reaction temperature can vary between 70°C and the reflux temperature of the solvent, and the reaction time varies between 3 and 48 hours. This reaction can also be carried out under phase transfer conditions by using an aqueous sodium hydroxide solution, toluene and a catalyst, such as Y:\fioin\NKI\Spccics\79 170.doc 3 tetrabutylammonium bromide. Under these conditions, the temperature of the reaction can vary between 50 0 C and 0 C, and the reaction time varies between 12 and 72 hours.

a NH 4VN a Np N F+ I N N C H CH,

F

c) By the reaction of 2-{4-[4-(4,5-dichloro- 2-methylimidazol-l-yl)butyl]piperazin-1-yl}-5-fluoropyrimidine and of a physiologically acceptable acid, such as citric acid, in a suitable solvent, such as ethanol.

CH, J S.The preparation of the novel compound which is the subject-matter of the invention and of its physiologically acceptable salts is shown in the following examples. A description is also given of some biological activities and some forms of use. The examples shown hereinbelow, given simply by way of illustration, must not, however, in any way limit the scope of the invention.

Example 1 Preparation of 2- chloro-2-methylimidazol-1-yl)butyl]piperazin-1-yl)dine A mixture of 3.17 g (0.01 mol) of 2-pyrimidinyl)-8-aza-5-azoniaspiro[4.5]decane, 2.26 g (0.015 mol) of 4,5-dichloro-2-methyl-1H-imidazole and 4 76 g (0.02 mol) of potassium carbonate in 80 ml of dimethylformamide is maintained at reflux for 12 hours.

The mixture is subsequently evaporated to dryness and the resulting crude product is redissolved in chloroform and washed repeatedly with water. The organic phase is dried and evaporated, and then the resulting crude product is purified by chromatography on a column of silica gel. 3.3 g (85% yield) of chloro-2-methylimidazol-1-yl)butyl]piperazin-1-yl}- 5-fluoropyrimidine are obtained in the form of an oil.

IR (film) cm1: 2944, 1610, 1555, 1503, 1449, 1402, 1361, 1243, 786.

1H NMR (CDC13, 300 MHz), 6: 1.54 2H), 1.73 2H), 2.34 3H) 2.38 2H), 2.43 4H) 3.74 4H), 15 3.85 2H), 8.15- 2H) Example 2 Preparation of chloro-2-methylimidazol-l-yl)butyl]piperazin-1-yl}- A mixture of 3.5 g (0.02 mol) of 2-(piperazin-l-yl)pyrimidine, 6.04 g (0.025 mol) of 1-(4-chlorobutyl)-4,5-dichloro-2-methyl-1H-imidazole and 4.14 g (0.03 mol) of potassium carbonate in 200 ml of dimethylformamide is maintained at reflux for 12 hours. The mixture is subsequently evaporated to dryness and the resulting crude product is redissolved in chloroform and washed repeatedly with water. The organic phase is dried and evaporated, and then the resulting crude product is purified by chromatography on a column of silica gel. 6.4 g (83% yield) of 2-{4-[4-(4,5-dichloro-2-methylimidazol-1-yl)butyl]are obtained in the form of an oil.

IR (film) cm1: 2944, 1610, 1555, 1503, 1449, 1402, 1361, 1243, 786.

iH NMR (CDC13, 300 MHz), 6: 1.54 2H), 1.73 2H), 2.34 3H) 2.38 2H), 2.43 4H) 3.74 4H), 3.85 2H), 8.15 2H).

5 Example 3 Preparation of chloro-2 -me thylimidazol-l-yl)butyl]piperazin-1-yl citrate 1.1 g (5.2 mmol) of citric acid monohydrate are added to a solution of 2 g (5.2 mol) of 2-{4-[4-(4,5-dichloro-2-methylimidazol-l-yl)butyl]in absolute ethanol.

After a certain period of time, 2.72 g (91% yield) of 2-{4-[4-(4,5-dichloro-2-methylimidazol-l-yl)butyl]piperazin-1-yl}-5-fluoropyrimidine citrate precipitate in the form of a solid with an M.p. of 151-153 0

C.

IR (KBr) cm-1: 3780-2270 (bb) 1707, 1616, 1482, 1429, 1375, 1244, 1214.

1 H NMR (d 6 -DMSO, 300 MHz), 6: 1.53 2H), 1.66 (m, 2H), 2.33 3H) 2.53-2.74 10H) 3.75 4H), 3.94 J 7.2 Hz, 2H), 8.46 2H).

ANTIEMETIC ACTIVITY: The compounds obtained in the invention are studied with regard to their effects on nausea in ferrets according to a method described by Costall et al. (Neuropharmacology, 1986, 25, 959-961).

Ferrets of both sexes (0.7-1.4 kg) are individually housed at 21 1 0 C and fed normally. The compound of the example or a vehicle is subsequently administered to them subcutaneously as pretreatment 15 minutes before the administration of copper sulfate S(CuSO 4 5H 2 0; 100 mg/kg by the intragastric route) or cisplatin (10 mg/kg i.v. by means of a permanent jugular cannula). The animals are observed at the beginning of vomiting and then for 30 minutes (copper sulfate) or 240 minutes (cisplatin). Vomiting is characterized by rhythmic abdominal contractions, either associated with the expulsion of solid or liquid matter (vomit) or not associated with the passage of matter via the mouth (nausea). The number of episodes and nausea or vomiting are recorded.

6 The compound of Example 3 is capable of altering the nausea induced by copper sulfate and of opposing the nausea induced by cisplatin.

a) Copper sulfate Compound Dose (mg/kg Number of episodes of nausea/vomiting Vehicle 8.2 1.4 Example 3 0.001 7.4 i 1.2 (NS) 0.01 3.2 0.6 0.1 1.0 0.2 1 1.7 0.3 p 0.05 compared with the values of the control vehicle b) Cisplatin Compound Dose (mg/kg Number of episodes of nausea/vomiting Vehicle 14.2 1.9 Example 3 0.001 13.8 2.4 (NS) 0.01 6.5 3.1 0.1 3.1 2.9 1 3.0 2.0 10 (C) p 0.05 compared with the values of the control vehicle STUDY OF TRAVEL SICKNESS IN THE SHREW (SUNCUS MURINUS) The compound of Example 3 is studied with regard to its effect on travel sickness (vertigo) induced in Suncus murinus according to the method described by N. Matsuki et al. ("Mechanisms and Control of Emesis", John Libbey Emotex, 1992, 233, 323-329).

Males of the species Suncus murinus weighing 58-72 grams are housed in groups of. 3 and are allowed free access to solid food and to water. One hour before the test, they are presented with cat food in their housing cage. At the beginning of the test, the animals receive the treatment with the test product by the i.p.

route. After 30 minutes, they are placed individually in the test boxes (15 x 15 x 10 cm). The boxes are 7 connected to a motor which displaces them over several wheels forward and backward with a frequency which induces nausea in them (travel sickness). This frequency is 1 hertz with an amplitude of displacement of 4 cm. The animals are left in the test boxes for minutes, in order for them to become used to their surroundings, and then the active movement of the cages is begun for 20 minutes, in order to determine the presence or the absence of nausea (without passage of matter from the digestive tract) and of vomiting (with passage of matter from the digestive tract).

The compound of Example 3 markedly inhibits the nausea caused by the movement (travel sickness), reducing both nausea and vomiting, between doses of 0.01 and 15 10 mg/kg by the i.p. route.

Compound Dose (mg/kg Number of episodes of nausea/vomiting Vehicle 10.0 1.39 Example 3 0.001 8.38 1.41 (NS) 0.01 0.33 0.21 0.1 2.17 0.83 0.33 0.21 10.0 0 0 (C) p 0.0001 compared with the values of the control vehicle ANXIOLYTIC AND ANTIDEPRESSANT ACTIVITY The anxiolytic and antidepressant activity is demonstrated by the affinity which the novel compounds exhibit for 5HTiA serotonin receptors Glitz, Drugs, 1991, 41, 11) and by means of the conditioned avoidance response test New et al., J. Med.

Chem., 1986, 29, 1476).

a) Binding to the 5HTIA serotonin receptor A rat hippocampus homogenate is used, a modification of the method of S.J. Peroutka (Neurochem., 1986, 47, 529) being followed. [3H]-8-HO-DPAT is used as radioligand and serotonin is used to measure the 8 nonspecific binding. The incubation time is 15 minutes at a temperature of 37°C. The radioligand bonded to the protein is separated by filtration through glass fiber filters and the radioactivity retained on the filter is determined by liquid scintillation. The inhibition constant (Ki, nM) is calculated by nonlinear regression analysis using the EBDA/LIGAND program (Munson and Rodbard, Analytical Biochemistry, 1980, 107, 220).

Compound of Example 3 Ki 19.4 nM b) Test of conditioned avoidance response In this test, use is made of male Wistar rats weighing 200 g trained to leap a barrier in a Letica 15 shuttle box (references LI 910 and LI 2700) during the 30 seconds which follow their introduction into the box.

Products having an anxiolytic or tranquilizing activity suppress the conditioned avoidance response.

Training: first day, 11 tests at intervals of 3 minutes. Electric shock in the paws at 30 seconds (5 mA, 0.1 s, 10 s) Second and third days: two daily tests, only with the selected rats {some of the grades of the first day (except the first test) 14}. Day of 25 the test: groups formed by the selected rats. Oral administration of the product or vehicle 45 minutes before the beginning of the study.

Compound of Example 3 ED 5 s 9.7 mg/kg, p.o.

INHIBITORY ACTIVITY WITH REGARD TO GASTRIC ACID

SECRETION

This activity is determined by means of the Shay procedure Shay et al., Gastroenterology, 1945, 43; Visscher et al., J. Pharmac. Exp. Ther., 1954, 110, 188) Male Wistar rats weighing from 200 to 250 g are used in this test, which rats are deprived of food up to the day preceding the test, with free access to water. Batches of 4 animals each are used, at least.

9 The rats are anesthetized with ethyl ether, a laparotomy is performed on them and their pylori are tied, and then an abdominal incision is made. The products are administered intraduodenally before suturing the abdominal incision. The dose administered in the first test is 40 mg/kg and then the median effective dose (EDs 0 is determined. The vehicle used is gum arabic at 5% w/v in doubly-distilled water.

Two hours after ligating the pylori, the rats are sacrificed by prolonged anesthesia with ethyl ether and the volume of gastric juice is measured. The total acidity is determined by means of a pH meter equipped with an automatic burette. For each product and for each dose tested, the percentage of inhibition of 15 gastric acid secretion is determined with respect to a reference batch.

Compound of Example 3 EDs 5 1.9 mg/kg, i.d.

The daily dosage in human or veterinary medicine is between 1 mg and 500 mg of product, which may be administered in one or more dispensations. The compositions are prepared in forms which are compatible Swith the administration route used, such as, for example, tablets, drag6es, hard gelatin capsules, suppositories, solutions or suspensions. These composi- 25 tions are prepared by means of known processes and they comprise from 1 to 60% by weight of the active principle and from 40 to 99% by weight of appropriate pharmaceutical vehicle compatible with the active principle and the physical form of the composition which is appropriate. By way of example, the formulae of three pharmaceutical dosage forms which comprise a product of the invention are presented.

Example of a tablet formulation Example 3 5 mg Lactose 60 mg Crystalline cellulose 25 mg Povidone K 90 5 mg Pregelatinized starch 3 mg 10 Colloidal silicon dioxide 1 mg Magnesium stearate 1 mg Total weight 100 mg Example of a hard gelatin capsule formulation Example 3 10 mg Polyoxyethylene glyceride 135 mg Glycerol behenate 5 mg Excipient 150 mg Example of an injectable preparation formulation Example 3 4 mg 8 mg Sodium chloride 15 mg 30 mg Water for injections q.s. for 2 ml .4 ml

Claims (2)

1. Use of the compound 2-{4-[4-(4,5-dichloro-2-methyl-imidazol-1-yl)butyl]- and its physiologically acceptable salts, of formula C1 C1 N F CH 3 or of the compound 2-{4-[4-(4,5-dichloro-2-methyl-imidazol-1 -yI)butyl]-1 citrate in the preparation of a medicament intended for the treatment of emesis. too

2. Method of treatment of emesis comprising administration of a therapeutically effective amount of the compound ,5-d ich loro-2-methyl- imidazol-1 -yl)butyl]-1 -piperazinyl)-5-fluoropyrimidine and its physiologically 15 acceptable salts of formula C1 C1 INN CH 3 or of the compound 2-{4-[4-(4,5-dichloro-2-methyl-imidazole-1 20 yl)butyl]piperazine-1 -yl}-5-fluoropyrimidime citrate. DATED: 24 January, 2003 PHILLIPS ORMVONDE FITZPATRICK Attorneys for: LABORATORIOS DEL DR ESTEVE S.A.