AU758797B2 - Virginiamycin mixture - Google Patents
Virginiamycin mixture Download PDFInfo
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- AU758797B2 AU758797B2 AU16372/01A AU1637201A AU758797B2 AU 758797 B2 AU758797 B2 AU 758797B2 AU 16372/01 A AU16372/01 A AU 16372/01A AU 1637201 A AU1637201 A AU 1637201A AU 758797 B2 AU758797 B2 AU 758797B2
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- mixture
- weight percent
- pharmaceutically acceptable
- virginiamycin
- feed grain
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Description
S&F Ref: 445892D1
AUSTRALIA
PATENTS ACT 1990 COMPLETE SPECIFICATION FOR A STANDARD PATENT
ORIGINAL
Name and Address of Applicant: Actual Inventor(s): Address for Service: Invention Title: Pfizer Inc.
235 East 42nd Street New York New York 10017 United States of America James William Carson, Frederic William Chapin and Charles Hollett Fahrenholz Spruson Ferguson St Martins Tower,Level 31 Market Street Sydney NSW 2000 Virginiamycin Mixture The following statement is a full description of this invention, including the best method of performing it known to me/us:ft 5845c VIRGINIAMYCIN MIXTURE Background of the Invention The present invention relates to a mixture comprising virginiamycin.
Virginiamycin antibiotics, in their most effective form, include both M and S components. Coccito, Micro. Rev., 43, 145 (1979). Virginiamycin has been used as an antibacterial and in the prevention of lactic acidosis United States Patents 5,137,900 and 5,242,938 and The Merck Index, 12 th Edition, pages 1702-1708). It has also been used in the form of a feed additive to improve growth in poultry, swine, and cattle. A possible mechanism for its use as a growth promotant could relate to an inhibition of intestinal flor. Coccito, supra. Virginiamycin's widely accepted use stems from having low toxicity, minimal production of resistant mutant strains, quick degradation in faeces, and minimal tissue retention.
Wettable powders have been used for the administration of various insecticides and herbicides.
S: 15s Summary of the Invention A first aspect of the present invention relates to a mixture comprising: a) virginiamycin; b) a pharmaceutically acceptable surfactant including sodium lauryl sulfate; c) a sufficient amount of a pharmaceutically acceptable buffering agent to provide a pH of from about 3.0 to about 7.0 when said mixture is added to water; and d) from about 0.5 weight percent to about 10.0 weight percent colloidal silicon dioxide, Swherein the ratio of weight percent of b) to the weight percent of a) is at least about 1.5:1.
25 A second aspect of the present invention provides feed grain treated with a water suspension of a mixture comprising: a) virginiamycin; b) a pharmaceutically acceptable surfactant including sodium lauryl sulfate; c) a sufficient amount of pharmaceutically acceptable buffering agent to maintain said suspension at a pH of from about 3.0 to about d) from about 0.5 weight percent to about 10.0 weight precent colloidal silicon dioxide, wherein the ratio of the weight percent of b) to the weight percent of a) percent by Sweight is at least about 1.5:1.
[R:\LIBA]05422.doc:jjp la- A third aspect of the present invention provides a method of treating feed grain with virginiamycin, comprising forming a mixture including: a) virginiamycin, b) a pharmaceutically acceptable surfactant including sodium lauryl sulfate, c) a sufficient amount of pharmaceutically acceptable buffering agent to provide a pH of from about 3.0 to about 7.0 when said mixture is added to water, and d) from about 0.5 weight percent to about 10.0 weight percent colloidal silicon dioxide, wherein the ratio of the weight percent of b) to the weight percent of a) percent by weight is at least about 1.5:1; suspending said mixture in water to form a suspension; and applying said suspension to feed grain.
A fourth aspect of the present invention provides feed grain treated by the method 15 of the third aspect of the present invention described above.
Detailed Description The mixture comprising virginiamycin is in the form of a wettable-type powder. The powder mixture can be added to water, including some forms of hard water, to produce a stable suspension of the virginiamycin, which can then be, for example, delivered directly to a patient orally) or applied to, for example, foodstuffs such as feed grain. The feed grain is then fed to animals, for example livestock and poultry, thereby administering the virginiamycin. The mixture should preferably be substantially 0 [R:\LIBA]05422.doc:ijp anhydrous so as to maximize the shelf life of the mixture prior to forming the water suspension.
Virginiamycin can be produced using known methods, for example, fermentation. For example, virginiamycin M can be produced by fermentation of a streptomyces species originally isolated from Indian soil samples and deposited with the Ar. rican Type Culture Collection, 12301-Parklawn Drive, Rockville, Maryland 20852, United States of America on July 29, 1986 and assigned ATCC number 53527, United States Patent 5,242,938.
Dose levels of virginiamycin are known in the art and can vary due to a number of factors, including, for example, compound activity, the route of administration, the time of administration, disease severity, excretion rate, combination of other drugs, as well as the age, body weight, sex, diet, and general health of the patient being treated.
Generally, for example, with a human subject, the daily effective dose can range from about 1.0 mg to about 1500 mg, preferably about 10 mg to about 500 mg, in single or divided doses. For a domestic animal, the effective dose can range from about 5 to about 200 parts per million per volume of food. United States Patent 5,242,938.
For veterinary purposes, dosage levels known in the art can also be found, for example, in the 1996 Feed Additive Compendium, Miller Publishing Co. (1996), pp.
324-328.
The claimed mixture comprises virginiamycin, preferably substantially pure, and a pharmaceutically acceptable surfactant, sodium lauryl sulfate (SLS), preferably substantially anhydrous and granulated. The ratio of the weight percent of SLS to the weight percent of virginiamycin should be at least about 1.5 to 1, preferably about 1.54 to 1. Substantially pure virginiamycin has an activity of about 200% (plus or minus 25 20%) as measured by methods known in the art (Gossele, et al., Analyst, 116, 1373 (1991) and Blain, et al., Analyst, 19, 361 (1994).
The powder mixture also includes a pharmaceutically acceptable anti-caking agent, colloidal silicon dioxide, which can act as scavenger for water as well as a wetting and suspending agent for the virginiamycin. The amount of colloidal silicon 30 dioxide can range from 0.5 weight percent of the mixture to about 10.0 weight percent of the mixture, preferably about 2.0 weight percent.
A pharmaceutically acceptable buffer is also included, for example phosphate or citrate buffers, preferably citric acid/sodium citrate. The amount of buffer should be sufficient to provide a pH of from about 3.0 to about 7.0, preferably from about 4.0 to about 5.5 when the mixture is added to water. Maintenance of pH permits the virginiamycin to be suspended in a water environment with minimal effect on its activity.
The powder mixture is added to water to form a stable suspension of the virginiamycin, which is then applied to, for example, feed grain. The amount of mixture dispensed in th,. water approximately 4.5% by weight of the suspension.
A pharmaceutically acceptable water soluble filler material can also be included.
Examples include sugars, such as lactose and dextrose, preferably lactose. Such fillers may also effect the flowability of the mixture.
A pharmaceutically acceptable anti-foaming agent, which is, for example, an emulsion and will not substantially effect the pH of the resulting suspension, can also be included in the anhydrous mixture, for example, polydimethylsiloxane. Once the suspension is made, the anti-foaming agent minimizes the amount of foam caused by the SLS upon agitation of the suspension. This can be important if, for example, the suspension is being sprayed and a pump is needed to drive the suspension through the spraying apparatus. The amount of anti-foaming agent in the mixture can range from about 0.5 weight percent to about 10.0 weight percent, preferably about 2.8 weight percent.
The mixture should preferably be maintained as substantially anhydrous prior to forming the suspension in order to minimize the breakdown of the components of the mixture. As a result of minimizing breakdown, the shelf life of the mixture can be maximized.
The mixture is formed by adding its ingredients together and thoroughly blending them together. Once the mixture is formed, a suspension can be made by 25 adding the mixture to water. The resulting suspension is substantially stable with minimal sedimentation of virginiamycin falling out of the suspension for about a day with periodic stirring or with little or no stirring of the suspension for about a couple hours, preferably about six hours once the suspension has been properly formed.
A pharmaceutically acceptable indicator dye can also be included, depending 30 upon acceptable food and drug regulations. The dye, for example, FD&C blue No. 1 powder, would give a blue color to the suspension and, in tum, to feed grain sprayed therewith. Thus, the treated grain can be readily identified.
A preferred formulation of the claimed invention is the following: INGREDIENT FORMULA W/W Virginiamycin Feed Grade* not available 18.8 (activity about 200%)1 Sodium Lauryl Sulfate C, 2
H
2 ,NaO,S 30.0 Colloidal Silicon Dioxide SiO 2 Citric Acid, anhydrous, fine granular C 6
HO
8 0 20.0 Sodium Citrate anhydrous powder C 6 HsNzO, 20.0 Antifoam AF emulsion [-(CH 3 2 SiOJn 2.8 FD&C Blue No. 1 powder FDA Lot AG4275 0.4 Wamer-Jenkinson Co. Code 05601 Lactose* anhydrous, 80 mesh C,,HO22 Virginiamycin and lactose calculated to provide 40% virginiamycin activity. Amounts will vary depending on purity of virginiamycin.
The following are intended as non-limiting examples of the present invention.
Example 1 The below listed ingredients were combined and mixed together until a uniform mixture was formed.
INGREDIENT wlv AMOUNT Virginiamycin Feed Grade 213% 0.94 9.40g Sodium Lauryl Sulfate 1.50 15.00g Colloidal Silicon Dioxide 0.10 1.00g Citric Acid U.S.P. 1.00 10.00g Sodium Citrate U.S.P. 1.00 10.00g Antifoam A.F. 0.10 1.00g A suspension was formed by adding the above mixture to 900 ml of distilled water in a 1 liter graduated cylinder until dispersion was complete. Then additional water was added to bring the total volume to 1 liter. pH 4.24.
Example 2 The below listed ingredients were combined and mixed together until a uniform mixture was formed.
INGREDIENT wlv AMOUNT Virginiamycin Feed GrAde 213% 0.94 9.40g Sodium Lauryl Sulfate 1.50 15.00g Colloidal Silicon Dioxide 0.10 1.00g Citrc Acid U.S.P. 1.00 10.00g Sodium Citrate U.S.P. 1.00 10.00g Antifoam A.F. 0.10 1.00g A suspension was formed by adding the above mixture to 900 ml of distilled water in a 1 liter graduate until dispersion was complete. Then additional water was added to bring the total volume to 1 liter. pH 4.30.
Example 3 The below listed ingredients were placed in a mixer and mixed for 15 minutes to form a base mixture.
INGREDIENT wlv
AMOUNT
Sodium Lauryl Sulfate 36.9 4500.0 g CricAd 24.6 3000.0 g Sodium Citrate 24.6 3000.0 g Anhydrous Lactose, 80 Mesh (Sheffield) 7.4 900.0 g Antifoam A. F. Emulsion 3.4 420.0 g 25 Colloidal Silicon Dioxide 2.6 300.0 g FD&C Blue No. 1 Powder 0.5 60.0 g A. 1126.8 g of virginiamycin animal feed grade (213% activity) was added to 4873.2 g of base mixture in a mixer and mixed for 15 minutes.
30 B. 956.1 g of virginiamycin animal feed grade (205% activity) was added to 3943.2 g of base mixture in a mixer and mixed for 15 minutes.
Claims (15)
1. A mixture comprising: a) virginiamycin; b) a pharmaceutically acceptable surfactant including sodium lauryl sulfate; c) a sufficient amount of pharmaceutically acceptable buffering agent to provide a pH of from about 3.0 to about 7.0 when said mixture is added to water; and d) from about 0.5 weight percent to about 10.0 weight percent colloidal silicon dioxide, wherein the ratio of the weight percent of to the weight percent of percent by weight 1o is at least about 1.5:1.
2. The mixture of claim 1, further including a pharmaceutically acceptable anti- foaming agent.
3. The mixture of claim 1, further including a pharmaceutically acceptable dye.
4. The mixture of claim 1, wherein said mixture is substantially anhydrous.
5. A virginiamycin mixture, substantially as hereinbefore described with reference to any one of the Examples.
6. Feed grain treated with a water suspension of a mixture comprising: a) virginiamycin; b) a pharmaceutically acceptable surfactant including sodium lauryl sulfate; c) a sufficient amount of pharmaceutically acceptable buffering agent to maintain said suspension at a pH of from about 3.0 to about d) from about 0.5 weight percent to about 10.0 weight percent colloidal silicon dioxide, wherein the ratio of the weight percent of to the weight percent of percent by weight 25 is at least about 1.5:1. i
7. The feed grain of claim 6, said mixture further including a pharmaceutically acceptable dye.
8. The feed grain of claim 6, further including a pharmaceutically acceptable anti- foaming agent in said mixture.
9. Feed grain, substantially as hereinbefore described with reference to any one of the Examples.
A method of treating feed grain with virginiamycin, comprising forming a mixture including: a) virginiamycin, b) a pharmaceutically acceptable surfactant including sodium lauryl sulfate, c) a sufficient amount of pharmaceutically acceptable buffering agent to provide a pH of from about 3.0 to about 7.0 when said mixture is added to water, and 7 d) from about 0.5 weight percent to about 10.0 weight percent colloidal silicon dioxide, wherein the ratio of the weight percent of to the weight percent of percent by weight is at least about 1.5:1; suspending said mixture in water to form a suspension; and applying said suspension to feed grain.
11. The method of claim 10, further including a pharmaceutically acceptable anti- foaming agent in said mixture.
12. The method of claim 10, further including a pharmaceutically acceptable dye in said mixture.
13. The method of claim 10, further including drying the feed grain after the applying step.
14. A method of treating feed grain with virginiamycin, substantially as hereinbefore described with reference to any one of the Examples.
15. Feed grain treated by the method of any one of claims 10 to 14. Dated 18 January 2001 Pfizer Inc. Patent Attorneys for the Applicant/Nominated Person SPRUSON FERGUSON e* o0 ego* 9. 5o o s< o. *oo
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AU16372/01A AU758797B2 (en) | 1996-07-01 | 2001-01-19 | Virginiamycin mixture |
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US60020512 | 1996-07-01 | ||
AU27862/97A AU2786297A (en) | 1996-07-01 | 1997-06-05 | Virginiamycin mixture |
AU16372/01A AU758797B2 (en) | 1996-07-01 | 2001-01-19 | Virginiamycin mixture |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
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AU27862/97A Division AU2786297A (en) | 1996-07-01 | 1997-06-05 | Virginiamycin mixture |
Publications (2)
Publication Number | Publication Date |
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AU1637201A AU1637201A (en) | 2001-04-12 |
AU758797B2 true AU758797B2 (en) | 2003-03-27 |
Family
ID=3716158
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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AU16372/01A Ceased AU758797B2 (en) | 1996-07-01 | 2001-01-19 | Virginiamycin mixture |
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Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
BE819863A (en) * | 1974-09-13 | 1975-03-13 | Microencapsulated virginiamycin compsns - coated with spermaceti contg. sodium lauryl sulphate |
-
2001
- 2001-01-19 AU AU16372/01A patent/AU758797B2/en not_active Ceased
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
BE819863A (en) * | 1974-09-13 | 1975-03-13 | Microencapsulated virginiamycin compsns - coated with spermaceti contg. sodium lauryl sulphate |
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