AU754118B2 - A process for the treatment of organophosphate poisoning - Google Patents

Description

WO 99/65501 PCT/NL98/00343 Title: A process for the treatment of organophosphate poisoning The invention relates to a process for the treatment of organophosphate poisoning.

The currently available treatment of organophosphate (OP)-poisoning (ir)reversible inhibition of acetylcholinesterase) is mainly based on a combined administration of a cholinesterase reactivator (oxime), a muscarinic receptor antagonist (atropine) and an anticonvulsant (diazepam). Experiments with primates in the past, however, have demonstrated that such a treatment, even when carried out immediately after OP-exposure, do not rapidly restore electroencephalographic (EEG) activity and fail to prevent neuronal brain damage and postintoxication incapacitation (Dawson et al 1995, Van Helden et al 1996, Busker et al 1996, Lalleman et al 1998, Shih and McDonough 1997). Moreover, clinical experience has indicated that oximes, although designed to reactivate the inhibited acetylcholinesterase (AChE), are not always sufficiently effective as reactivators even when administered at very high dosages (Van Helden et al 1996). Furthermore, none of the oximes can be regarded as a 'broad spectrum' (generic) antidote, effective against all currently existing nerve agents.

Therefore, it seems justified to conclude that the therapeutic efficacy of available oximes in nerve gas poisoning is less than optimal, especially in case of rapidly ageing (a process leading to oxime-resistance) nerve agents such as soman, and seems to have reached its limits (Dawson et al 1995; Van Helden et al 1996). Therefore, new and preferably generic approaches are necessary to improve protection.

Accumulation of acetylcholine (ACh) in the synaptic cleft is generally considered as the main cause of the symptoms that ultimately lead to death in case of OPpoisoning. Thus, it is proposed that drug-induced decrease of WO 99/65501 PCT/NL98/00343 2 ACh-release in brain and muscle can prevent and counteract convulsions that are a result of OP-poisoning and improve survival rate.

In accordance with the present invention, it has been found that adenosine Ai receptor agonists are well-suited for effectuating a decrease of ACh-release in the brain and in muscles. Hence, the invention provides a process for treating organophosphate poisoning in a mammal comprising the administration of an Ai receptor adenosine agonist.

When compared with oxime treatment, the present approach provides a generic protection, i.e. protection against all nerve agents (including soman, sarin, tabun, VX and many other AChE-inhibitors, such as insecticides and pesticides), independent of ageing of the inhibited AChE. A process according to the invention is directed towards accumulation of ACh which causes the symptoms and death in OP-poisoning. Furthermore, lower dosages than in the oxime treatment are required in order to achieve an effective treatment.

Endogenous adenosine elicits a large variety of physiological effects through interaction with cell-surface adenosine receptors, which are heterogeneous (Al, A 2 A, A 2 B and A3 receptors) and widely spread throughout the body (Collis and Hourani 1993). This large variety of physiological effects elicited by adenosine provides a potential for therapeutic application of adenosine analogues.

Adenosine itself has been registered under the name of Adenocard as an anti-arrythmic drug and for controlled hypotension during aneurysm surgery. Al adenosine agonists have been proposed as sedatives, in supraventricular tachycardia, in type II diabetes, stroke and seizures, whereas A2 adenosine agonists have been proposed as inhibitors of aggregation in thrombosis, in diagnosis of diseases in coronary arteries, in ischemia and reperfusion.

Adenosine agonists for the A3 receptor have been proposed for use in certain behavorial disturbances. Other conditions for 3 which activation of the adenosine receptors may be useful are inflammation and some pathophysiological situations, such as anxiety and panic attacks, sleep disturbances, schizophrenia, depression, epilepsy and convulsions.

Inherent to the widespread distribution of adenosine receptor subtypes is the difficulty in obtaining desirable drug actions without concomitant side effects. For example, the profound hemodynamic disturbances observed with adenosine Ai and A2A agonists have limited their use.

A new target is the inhibition of ACh-release to counteract organophosphate poisoning. This release inhibition is mediated by the Ai adenosine receptor.

All references, including any patents or patent applications, cited in this specification are hereby 15 incorporated by reference. No admission is made that any reference constitutes prior art. The discussion of the references states what their authors assert, and the applicants reserve the right to challenge the accuracy and pertinency of the cited documents. It will be clearly 20 understood that, although a number of prior art publications are referred to herein, this reference does not constitute an admission that any of these documents forms part of the common general knowledge in the art, in Australia or in any other country.

For the purposes of this specification it will be clearly understood that the word "comprising" means "including but not limited to", and that the word "comprises" has a corresponding meaning.

The present invention provides a process for treating organophosphate poisoning in a mammal comprising the administration of an Ai receptor adenosine agonist. In the context of the invention, an Ai receptor adenosine agonist is an adenosine agonist which is selective for the Ai receptor, i.e.

H:\bridgetg\keep\Speci\p40227.doc response.doc 04/09/02 3a interacts predominantly with the Ai receptor, particularly at lower dosages.

In addition, it has been found that application of adenosine agonists with reduced intrinsic activity, i.e. partial Ai adenosine agonists, is highly beneficial. In the context of the invention, a partial agonist is a compound that has a submaximal physiological effect at complete receptor occupancy in a certain system. It has been found that the activity of these drugs not only depends on receptor subtypes but on tissue differences (tissue selectivity) as well (Kenakin 1993). This results in less pronounced cardiovascular actions and a potential increase in selectivity of action, the o inhibition of ACh-release in the brain.

Accordingly, in a preferred embodiment the present 15 invention is directed to the treatment of organophosphate poisoning in mammals comprising the administration of a partial Ai adenosine agonist. In accordance with this preferred embodiment, severe adverse effects of the treatment with respect to blood pressure and heart rate can be significantly reduced.

H:\bridgetg\keep\Speci\p40227.doc response.doc 04/09/02 WO 99/65501 PCT/NL98/00343 4 Particularly preferred partial Ai adenosine agonists for use in a process according to the invention are 8alkylamino-substituted analogues of N 6 -cyclopentyladenosine, 8-substituted adenosine, 8-substituted theophylline-7-ribose analogues, and deoxyribose analogues of N 6 cyclopentyladenosine (CPA), N 6 -cyclohexyladenosine (CHA), N 6 R-phenylisopropyladenosine (R-PIA) and N 6

-S-

phenylisopropyladenosine (S-PIA). These adenosine agonists have a highly beneficial therapeutic window. In other words, they combine a high activity and therapeutic efficacy with a low toxicity.

Suitable examples of the class of 8-alkylaminosubstituted analogues of N 6 -cyclopentyladenosine have the formula (I)

NH

N

N

N N HO OH OH wherein R is -NHCH 3

-NHCH

2

CH

3

-NH(CH

2 2

CH

3

-NH(CH

2 3

CH

3 or -NH-cyclopentyl. These compounds may be prepared in any known manner Roelen et al 1996).

Suitable examples of the class of deoxyribose analogues of N 6 -cyclopentyladenosine (CPA), N 6 cyclohexyladenosine (CHA), N 6 -R-phenylisopropyladenosine

(R-

PIA) and N 6 -S-phenylisopropyladenosine (S-PIA) have the formula (II) WO 99/65501 PCT/NL98/00343

R'NH

N

N< N

HO

X

2 X

(II),

wherein R is cyclopentyl, cyclohexyl, R-phenylisopropyl, or S-phenylisopropyl, and wherein X, and X, are different from each other and chosen from hydrogen and hydroxyl. These compounds have been described by Van der Wenden et al 1995a.

Suitable 8-substituted adenosines and 8-substituted theophylline-7-ribose have for instance been described by Van der Wenden et al 1995b. Preferred 8-substituted adenosines have the formula (III)

NH

2 N: N

HO

OH OH (III), wherein R is methyl, ethyl, vinyl, thiophenyl, hydroxyl, oxymethyl, amino, aminoalkyl with from 1 to 5 carbon atoms, aminoalkylamine with from 1 to 5 carbon atoms, aminocyclopentyl, cyclohexyl, or halogen.

Preferred 8-substituted theophylline-7-riboses have the formula (IV)

CH

3 N NCH 3

HOO

OH OH (IV), wherein R is hydrogen, amino, aminoalkyl with from 1 to 7 carbon atoms, or aminophenyl.

It will be clear that it is also possible to use suitable combinations of the above A, adenosine agonists for WO99/65501 PCT/NL98/00343 6 treating an OP-poisoning. A treatment comprising such a combined administration of A, adenosine agonist is also encompassed by the invention.

As has been mentioned above, one of the great advantages of a process according to the invention is that it is a generic process. This means that it can be used to treat organophosphate poisoning resulting from substantially all nerve agents, such as soman, sarin, tabun, VX and so forth, as well as other AChE-inhibitors, such as a large number of insecticides and pesticides. An organophosphate poisoning has been found to be based on the inhibition of the enzyme acetylcholinesterase (AchE). Inactive AChE cannot hydrolyze acetylcholine (Ach) which will accumulate in the cholinergic synaps and as a result will paralyze the synaptic transmission. Apparent, outward symptoms are salivation, convulsions and respiratory paralysis.

The treatment of the invention can be applied to any mammal suffering from the effects of an OP-poisoning.

However, it will be mostly applied to primates, in particular to humans.

As the effects of an OP-poisoning can be lethal within a very short period of time after the intoxication, i.e. the exposure to the poisonous compound(s), it is preferred that the treatment according to the invention is performed as soon as possible after said exposure. Desirably, the administration of an A, receptor adenosine agonist in accordance with the invention is carried out within one minute after acute intoxication or on guidance of symptoms.

First mild symptoms are fatigue, headache, dizziness, numbness of extremities, nausea and vomiting, sweating, extreme salivation, diarrhoea, abdominal pain, frequent urination, and miosis. Moderate symptoms are generalized weakness, speech impediment, fasciculations, trembling, miosis, hampered motoric fasciculations, fever, tightness in the chest, involuntary urination and defecation.

WO 99/65501 PCT/NL98/00343 7 The dosage in which the A, adenosine agonist can suitably be administered may vary within the range of 0.1-20 mg/kg, but is highly dependent on efficacy and adverse effects. Preferably, the dosage is chosen within the range of 1-2 mg/kg.

Effective manners in which the A, adenosine agonist may be administered include intramuscular, intravenous, and intranasal administration. The most preferred manners of administration are intramuscular and intravenous administration since after these application routes the A, adenosine agonist reaches the site of the A, receptor, where it is intended to effect a decrease of ACh-release, very fast.

For the above manners of administration, the A, adenosine agonist can most suitably be formulated in the form of a saline solution. However, in case the A, adenosine agonist appears insufficiently soluble in water, it may be useful to formulate them in DMSO or ethanol, diluted .with a solution of sodium chloride in water (saline) to a final to 30 vol.% DMSO solution, or a 5-10 vol.% ethanol solution.

OP-poisoning will be mostly encountered by people under harsh conditions, e.g. soldiers at war, anti-terrorist squads, and so forth. Moreover, it is of great importance that the treatment in accordance with the invention is performed as soon as possible after exposure to the poison.

For these reasons, it is highly preferred to use a so-called 'auto-injector' device for the administration of the drug.

This device has for instance been developed by Astra Tech AB, M61ndal, Sweden and by Meridian Medical Technologies, Columbia, Maryland, USA. In order to administer an A, receptor adenosine agonist using an auto-injector, the autoinjector is put on a muscle a thigh muscle), and after pressing a button, a hollow needle penetrates the skin into the muscle and a unit-dose of the desired A receptor adenosine agonist is injected into the muscle. Thus, the invention also encompasses an auto-injector holding a WO 99/65501 PCT/NL98/00343 8 formulation comprising an Ai receptor adenosine agonist as disclosed hereinabove.

The invention will now be elucidated by the following, non-restricting examples.

Example I An experiment was carried out using ethylcarboxamido-adenosine (NECA), an agonist of A, and A 2 receptors, to treat OP-poisoning, using the nerve agent soman as an irreversible model AChE-inhibitor.

Based on in vitro experiments in which 300 nM of NECA appeared to decrease the release of ACh from a rat diaphragm endplate zone by 70%, NECA was tested in an in vivo experiment. The in vitro concentration was extrapolated to an in vivo dose. Assuming that NECA distributes homogeneously in the body and that 300 nM is the effective concentration in the brain (300 nM 300 nMol/lit/kg 0.1 mg/kg 0.1 mg/kg of NECA was administered intramuscularly In a practical protocol, this calculated dose was administered intramuscularly at 1 min following a subcutaneous poisoning with 1.5 or 2 LD 50 soman in unanaesthesized rats. Symptoms and survival were registered. The results of this pilot are presented in Table 1 and show that 0.1 mg/kg

NECA

prevents and postpones the appearance of convulsive activity, and tends to improve the survival rate.

Example II In this experiment cyclopentyl adenosine (CPA), a highly specific A, adenosine receptor agonist, was tested using a similar protocol as described in Example I. The therapeutic efficacy of two doses (1 and 2 mg/kg, of the latter compound was tested against 1.9 LDs 0 soman in a similar way as described in Example I for NECA. The results are presented in Table 2 showing that administration of CPA prevented convulsions and led to survival of each animal in a healthy condition judging from clinical observation.

WO 99/65501 PCT/NL98/00343 9 Example III In separate experiments, using a limited number of unanaesthesized rats, it was investigated to what degree CPA (2 mg/kg would be able to prevent the accumulation of ACh in the striate body (corpus striatum) of the brain upon soman poisoning (2 LD, 0 by performing brain microdialysis according to the method described by Moor et al (1994). Briefly, rats were anaesthetized with chloral hydrate (400 mg/kg and a dialysis probe was implanted in the striate body. The actual dialysis experiments were conducted around 20 h after surgery.

Probes were perfused with artificial cerebrospinal fluid containing in mM: NaCl 147, KC1 3.0, CaCI 2 1.2, and MgC1 2 1.2. The AChE-inhibitor neostigmine bromide (10 8 M) was added to this perfusion fluid to obtain detectable quantities of base-line ACh. The artificial cerebrospinal fluid was delivered by a syringe pomp (Carnegy Medicine, Sweden) at a rate of 2 ±l/min. Ten min samples were collected in a loop of an injection valve that was automatically activated by an electronic timer.

After stabilisation of the ACh levels, soman (1-2 LDs) was injected subcutaneously followed by intramuscular injection of CPA (2 mg/kg) 1 minute later. These preliminary results demonstrated a low level of extracellular brain ACh (0-50 fold increase in ACh) following CPA treatment of soman poisoning, which was in contrast to a large increase in the amount of extracellular ACh in soman-poisoned animals not treated with CPA (180-400 fold increase in ACh). This low level of ACh-release in the brain following soman poisoning and CPA treatment was associated with postponement or lack of symptoms, and survival of the animals. Soman poisoned animals (controls) showed convulsions and died within 20 minutes.

Example IV A number of partial A, receptor agonists was tested in a similar way as described for NECA and CPA in Examples

I

WO 99/65501 PCT/NL98/00343 and II. Advantageous therapeutic efficacy against soman and sarin in rats and-guinea pigs was demonstrated while the adverse effects on blood pressure and heart rate were less than in case of NECA and CPA.

The protocol in which the efficacy of the partial A, receptor agonists against AChE-inhibitors was tested, was not standard; both repetitive and prophylactic administration (intramuscularly or intravenously) were investigated. Neither the level of intoxication was standard; it was in the range of 0.5-3 LDs 0 of AChE-inhibitors tested.

WO 99/65501 PCT/NL98/00343 11 Table 1. Protection of NECA (0.1 mg/kg intramuscularly) injected 1 min. after soman-poisoning in rats (n indicates the number of animals tested).

Treatment Survival time Symptoms Soman 15 min. starting at 5-7 min.: (2 LD 50 salivation, convulsions, respiratory distress Soman (2 LD 50 24 h; normal respiration; NECA 24 h; alert, dry mouth, drank water; 33 min.; convulsions at t=21 min.; min.; convulsions at t=50 min.; min. convulsions at t=40 min.

Soman (1.5 24 h starting at 2-10 min.:

LD

50 convulsive activity for hours on end Soman (1.5 24 h no convulsions at all LDs, s.c.)

+NECA

(n=3) NECA (n=2) 24 h no pnysical signs WO 99/65501 PCTINL98/00343 12 Table 2. Protection of CPA (intramuscular) injected 1 min.

after soman-poisoning (1.9 LDso in rats (n indicates the number of animals tested).

Treatment Survival time Symptoms Soman 17-28 min. after 5-7 min.: chewing, salivation, convulsions, respiratory distress Soman CPA 24 h 3 rats: no symptoms; (1 mg/kg) 1 rat: chewing after 32 min., then salivation, convulsions and decreased respiration; 1 rat: decreased respiration frequently, and salivation next morning; 1 rat: chewing after 10 min.

Soman CPA 24 h 5 rats: no symptoms, normal (2 mg/kg) respiration, alert, dry mouth, drank water; 1 rat: some traces of blood around mouth next morning.

CPA (2 mg/kg) (n=2) 24 h no physical s WO 99/65501 PCT/NL98/00343 13 References *Busker RW, Zijlstra JJ, Philippens, Groen B, Melchers BPC (1996) Comparison of the efficacy of single or repeated HI-6 treatment following soman poisoning in guinea pigs and marmoset monkeys. Toxicol 112: 183-194 *Collis MG and Hourani SMO (1993) Adenosine receptor subtypes. TiPS 14: 360-366 *Correio-de-Sa P, Sebastiao AM, Ribeiro JA (1991) Inhibitory and excitatory effects of adenosine receptor agonists on evoked transmitter release from phrenic nerve ending of the rat. Br J Pharmacol 103 92): 1614-1620 *Dawson RM (1994) Review of oximes available for treatment of nerve agent poisoning. J Appl Toxicol 14:317-31 *Kenakin TP (1993) Pharmacological analysis of drugreceptor interaction, pp441-46 8 Raven Press, New York *Lallement G, Clarencon D, Masqueliez C, Baubichon D, Galonnier M, Burckhart M-F, Peoc'h M, Mestries J-C (1998) Nerve agent poisoning in primates: antilethal, anti-epileptic and neuroprotective effects of GK-11. Arch Toxicol 72:84-92 Moor E, De Boer P, Beldhuis HJA, Westerink

BHC

(1994) A novel approach for studying septo-hippocampal cholinergic neurons in freely moving rats: a microdialysis study with dual-probe design. Brain Res 648: 32-38 *Roelen H, Veldman N, Spek AL, Von Frijtag Drabbe Kunzel JK, Mathot RAA, IJzerman AP (1996) N6, C8disubstituted adenosine derivatives as partial agonists for adenosine Al receptors. J Med Chem 39: 1463-1471 WO 99/65501 PCT/NL98/00343 14 *Sebastiao AM, Stone TW, Ribeiro JA (1990) The inhibitory adenosine receptor at the neuromuscular junction and hippocampus of the rat: antagonism by 1,3,8-substituted xanthines. Br J Pharmacol 101, 453-459 *Shih TM, McDonough JH (1997) Neurochemical mechanisms in soman-induced seizures. J Appl Toxicol 17: 255- 264 *Van der Wenden EM, Von Frijtag Drabbe Kunzel JK, Mathot RAA, Danhof M, IJzerman AP, Soudijn W (1995a) Ribosemodified adenosine analogs as potential partial agonists for the adenosine receptor. J Med Chem 38: 4000-4006 *Van der Wenden EM, Hartog-Witte HR, Roelen HCPF, Von Frijtag Drabbe Kunzel JK, Pirovano IM, Mathot RAA, Danhof M, Van Aerschot A, Lidaks MJ, IJzerman AP, Soudijn W (1995b) 8- Substituted adenosine and theophylline-7-riboside analogs as potential partial agonists for the adenosine Al receptor. Eur J Pharmacol Mol Pharmacol Sect 290: 189-199 *Van Helden HPM, Busker RW, Melchers BPC, Bruijnzeel PLB (1996) Pharmacological effects of oximes: how relevant are they? Arch Toxicol 70:779-786 *Van Schaick EA, Mathot AA, Gubbens-Stibbe

JM,

Langemeijer MWE, Roelen HCPF, IJzerman AP, Danhof M (1997) 8- Alkylamino-substituted analogs of N6-cyclopentyladenosine are partial agonists for the cardiovascular adenosine Al receptors in vivo. J Pharmacol Exp Ther 283: 800-808

Claims (16)

1. A process for treating organophosphate poisoning in a mammal comprising the administration of an A, receptor adenosine agonist.

2. A process according to claim 1, wherein a partial A, receptor adenosine agonist is administered.

3. A process according to claim 2, wherein the partial A, receptor adenosine agonist is chosen from the group of 8- alkylamino-substituted analogues of N 6 -cyclopentyladenosine,

8-substituted adenosine, 8-substituted theophylline-7-ribose analogues, and deoxyribose analogues of N 6 cyclopentyladenosine (CPA), N 6 -cyclohexyladenosine (CHA), N 6 R-phenylisopropyladenosine (R-PIA) and N 6 -S- phenylisopropyladenosine. 4. A process according to claim 3, wherein the partial A, adenosine agonist is a 8-alkylamino-substituted analogue of N 6 -cyclopentyladenosine having the formula (I) NH RN NR N HOQ OH OH wherein R is -NHCH 3 -NHCH 2 CH 3 -NH(CH) 2 CH 3 -NH(CH 2 3CH 3 or -NH-cyclopentyl. A process according to claim 3, wherein the partial Al adenosine agonist is a deoxyribose analogue of N 6 cyclopentyladenosine (CPA), N6-cyclohexyladenosine (CHA), N 6 R-phenylisopropyladenosine (R-PIA) or N6-S- phenylisopropyladenosine having the formula (II) 16 R.NH N HO, Q X 2 X 1 (II), wherein R is cyclopentyl, cyclohexyl, R-phenylisopropyl, or S- phenylisopropyl, and wherein X, and X 2 are different from each other and chosen from hydrogen and hydroxyl. 6. A process according to claim 3, wherein the partial Ai adenosine agonist is an 8-substituted adenosine having the formula (III) 0. NH 2 R O HO OH OH (III), wherein R is methyl, ethyl, vinyl, thiophenyl, hydroxyl, 10 methyoxy, amino, aminoalkyl with from 1 to 5 carbon atoms, aminoalkylamine with from 1 to 5 carbon atoms, aminocyclopentyl, cyclohexyl, or halogen. 7. A process according to claim 3, wherein the partial Ai adenosine agonist is an 8-substituted theophylline-7-ribose having the formula (IV) CH3 N N NR "N'CH 3 HO 0 OH OH (IV), wherein R is hydrogen, amino, aminoalkyl with from 1 to 7 carbon atoms, or aminophenyl. 8. A process according to any one of claims 1 to 7, wherein a human is treated for organophosphate poisoning. H:\bridgetg\keep\Speci\p40227.doc response.doc 04/09/02 17

9. A process according to any one of claims 1 to 8, wherein the Ai receptor adenosine agonist is administered in a dosage of 0.1-20 mg/kg. A process according to any one of claims 1 to 9, wherein the Ai receptor adenosine agonist is administered intramuscularly or intravenously.

11. A process according to claim 10, wherein the Ai receptor adenosine agonist is administered in the form of a saline solution.

12. A process according to claim 11, wherein the saline solution further comprises 10-30 vol.% dimethylsulfoxide.

13. A process according to claim 11 or 12, wherein the saline solution further comprises 5-10 vol.% ethanol.

14. A process according to any one of claims 1 to 13, 15 wherein the Ai receptor adenosine agonist is administered by use of an injector, preferably an auto-injector.

15. An injector, preferably an auto-injector, comprising an Ai receptor adenosine agonist.

16. Use of an Ai receptor adenosine agonist for preparing a medicament for treating organophosphate poisoning in mammals.

17. A method of treating organophosphate poisoning in mammals comprising the step of administering to a subject in need thereof an effective amount of an Ai receptor adenosine agonist.

18. A process according to claim 1, substantially as herein described with reference to any of the examples.

19. An injector according to claim 15, substantially as herein described with reference to any of the examples.

20. A use according to claim 16, substantially as herein described with reference to any of the examples. H:\bridgetg\keep\Specixp40227.doc response.doc 04/09/02 18

21. A method according to claim 17, substantially as herein described with reference to any of the examples. Dated this 4th day of September 2002 NEDERLANDSE ORGANISATIE VOOR TOEGEPASTNATUURWETENSCHAPPELIJK ONDERZOEK TNO By their Patent Attorneys GRIFFITH HACK Fellows Institute of Patent and Trade Mark Attorneys of Australia see- H:\bridgetg\keep\Speci\p4O227.doc responsedoc 04/09/02