AU7526791A - Selective amplification system using q beta replicase - Google Patents

Selective amplification system using q beta replicase

Info

Publication number
AU7526791A
AU7526791A AU75267/91A AU7526791A AU7526791A AU 7526791 A AU7526791 A AU 7526791A AU 75267/91 A AU75267/91 A AU 75267/91A AU 7526791 A AU7526791 A AU 7526791A AU 7526791 A AU7526791 A AU 7526791A
Authority
AU
Australia
Prior art keywords
rna
replication
agent
mutant
beta replicase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU75267/91A
Inventor
James E. Stefano
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gene Trak Systems Corp
Original Assignee
Gene Trak Systems Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gene Trak Systems Corp filed Critical Gene Trak Systems Corp
Publication of AU7526791A publication Critical patent/AU7526791A/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Treating Waste Gases (AREA)
  • Amplifiers (AREA)

Abstract

Mutant forms of midivariant RNA are disclosed which are replicated efficiently by the enzyme Q beta replicase in the presence of an agent(s) which is useful for the real time, quantitative detection of the accumulating RNA products. This agent(s) also suppresses the replication of non-mutant wild type midivariant RNA. The mutant MDV RNAs, when modified by the addition of specific probe sequences, retain their replication properties in the presence of the detection agent. This method allows the replication to be monitored using a real time mode thereby providing a reliable method for monitoring the kinetics of the reaction, and determining the initial level of amplified RNA.
AU75267/91A 1990-04-25 1991-04-22 Selective amplification system using q beta replicase Abandoned AU7526791A (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US51416190A 1990-04-25 1990-04-25
US51451890A 1990-04-25 1990-04-25
US514161 1990-04-25
US514518 1990-04-25

Publications (1)

Publication Number Publication Date
AU7526791A true AU7526791A (en) 1991-11-07

Family

ID=27058117

Family Applications (1)

Application Number Title Priority Date Filing Date
AU75267/91A Abandoned AU7526791A (en) 1990-04-25 1991-04-22 Selective amplification system using q beta replicase

Country Status (6)

Country Link
EP (1) EP0454461B1 (en)
JP (1) JP3240151B2 (en)
AT (1) ATE139575T1 (en)
AU (1) AU7526791A (en)
CA (1) CA2040503A1 (en)
DE (1) DE69120342T2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU694199B2 (en) * 1990-12-31 1998-07-16 Promega Corporation Nucleic acid amplification with DNA-dependant RNA polymerase activity of RNA replicases

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5464744A (en) * 1992-09-24 1995-11-07 Norval B. Galloway Methods and compositions for reducing false positive signals in an RNA amplification system
US6534273B2 (en) 1997-05-02 2003-03-18 Gen-Probe Incorporated Two-step hybridization and capture of a polynucleotide
AU2379000A (en) 1999-12-22 2001-07-03 Procter & Gamble Company, The Disposable garment comprising meltblown nonwoven backsheet
IL160229A0 (en) 2001-08-10 2004-07-25 Genset Sa Polynucleotides, polypeptides encoded thereby and their use
EP1711517A4 (en) 2004-01-21 2008-02-13 Univ Utah Res Found MUTANT SODIUM CHANNEL NAv1.7 AND METHODS RELATED THERETO
WO2011084685A2 (en) 2009-12-17 2011-07-14 Children's Medical Center Corporation Saposin-a derived peptides and uses thereof
JP2013545439A (en) 2010-09-17 2013-12-26 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ Functional genomics assay to characterize the usefulness and safety of pluripotent stem cells
WO2013003112A1 (en) 2011-06-27 2013-01-03 The Jackson Laboratory Methods and compositions for treatment of cancer and autoimmune disease
US10378060B2 (en) 2011-10-14 2019-08-13 Dana-Farber Cancer Institute, Inc. ZNF365/ZFP365 biomarker predictive of anti-cancer response
WO2014200905A2 (en) 2013-06-10 2014-12-18 President And Fellows Of Harvard College Early developmental genomic assay for characterizing pluripotent stem cell utility and safety
WO2014205374A1 (en) 2013-06-20 2014-12-24 The Trustees Of The University Of Pennsylvania Methods for diagnosing pancreatic cancer

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1339981C (en) * 1984-05-25 1998-08-04 Fred R. Kramer Replicatable hybridizable recombinant rna probes and methods of using same
JP3276955B2 (en) * 1988-09-30 2002-04-22 ジーン−トラック・システムス Probe structure bound to end of RNA template and method of using the same

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU694199B2 (en) * 1990-12-31 1998-07-16 Promega Corporation Nucleic acid amplification with DNA-dependant RNA polymerase activity of RNA replicases

Also Published As

Publication number Publication date
CA2040503A1 (en) 1991-10-26
EP0454461A1 (en) 1991-10-30
DE69120342T2 (en) 1996-12-19
ATE139575T1 (en) 1996-07-15
JP3240151B2 (en) 2001-12-17
EP0454461B1 (en) 1996-06-19
DE69120342D1 (en) 1996-07-25
JPH04234999A (en) 1992-08-24

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