AU7498700A - Bran products and methods for production thereof - Google Patents

Description

WO 01/21012 PCT/AUOO/01113 1 BRAN PRODUCTS AND METHODS FOR PRODUCTION THEREOF Technical Field The present invention relates generally to methods of producing 5 improved bran products from cereals and in particular to an improved oat bran product and its method of production. Background Art The nutritional advantages of cereals such as oats, barley, wheat. rice, 10 maize and millet are well known. One of the key components of cereal that contributes to the nutritional aspects is dietary fibre. Foods rich in dietary fibre have been thought to result in benefits ranging from reduction in the risk of cancer to the reduction in cholesterol levels. Dietary fibre has been considered an important food source and has 15 been found to play an important role in the prevention of certain large intestine diseases, including cancer of the colon and diverticulitis. Total dietary fibre (TDF) comprises both soluble dietary fibre and insoluble dietary fibre and is considered to be the soluble and insoluble components of food that are not digested by enzymes in the human gastrointestinal tract. Oats 20 and barley have relatively high levels of soluble fibre which is predominantly in the form of p-glucan. The soluble fibre content of barley is roughly the same as that of oats although: the oat bran fraction is distinctly higher in soluble fibre than barley bran. There has. therefore. been considerable interest in the processing of oats to produce oat bran. 25 Oat bran has been defined by the American Association of Cereal Chemists (AACC 1989) as the food which is produced by grinding clean oat groats or rolled oats and separating the resulting fractions by sieving or other mechanisms. The oat bran fraction is not more than 50% of the starting material. and has a total of p-glucan content of at least 5.5% (dry weight 30 basis) and a total dietary fibre content of at least 16% (dry weight basis), and such that at least one third of the total dietary fibre is soluble fibre. A key characteristic of oat bran is that unlike most other cereal brands, it contains large amounts of adhering endosperm with accompanying significant concentrations of p-glucan and starch. Oat bran is a useful source of 35 complex carbohydrate in the diet and has a specific effect attributable to the soluble fibre, or p-glucan.

WO 01/21012 PCT/AUOO/01113 2 The commercial oat fraction that is commonly referred to as "bran" consists of the outer layers of the groat (pericarp, seed coat, nucellus and aleurone layers) plus one to several cell thickness of adhering starchy endosperm containing starch, protein and -glucan. 5 p-Glucan is found in the seed of cereals and other grasses, but is present in the highest concentrations in oats and barley. The P-glucan content of oats typically falls within the range of 25-60g/kg. The highest concentration of P-glucan is found in the bran fraction, specifically in the aleurone and sub-aleurone cell layers. P-Glucan has recently been 10 recognised as a useful soluble dietary fibre. and as a useful texturing agent for foods. As well as being a nutritional food, oats have been found to be beneficial in the prevention of or amelioration of disease states, including improvements in gastro-intestinal function, modulation of glucose metabolism,. and reducing blood cholesterol. As P-glucan is the likely active 15 principal in oat bran, there has been a search for technologies that will yield oat bran fractions enriched in P-glucan. Cereal grains such as oats are also rich in essential lipids including monounsaturated and polyunsaturated fats. Such fats are an important requirement in the every day diet of humans and animals. 20 Attempts to produce oat bran in ways analogist to those used for wheat bran have met with limited success. Oats have a soft kernel and a relatively high content of lipid in the endosperm. which leads to the clogging of rollers and sieves. Although many relatively hard-textured cereals (eg wheat, barley. corn. sorghum) can be dry milled to some extent in roller or abrasive 25 mills to produce concentrates of outer grain tissues (eg bran), the soft texture and usual high lipid levels of the oat kernel preclude such manipulations. The endosperm (flour) does not easily separate from the outer layers and the lipids contribute to a product that is not easily sieved. Typically, therefore, commercial oat bran products contain large amounts of adhering starchy 30 endosperm. Commercial oat bran is therefore not an anatomically pure bran which is a sieved fraction that is enriched in p-glucan. Commercial oat bran production remains essentially a dry grinding and sifting operation. The process generally begins with the cleaning of the incoming seeds to remove foreign matter. detaching the hull from the groat, 35 subjecting the groats to a process to inactivate enzymes and develop flavour followed by steaming and flaking. Groats contain 6 to 9% fat, which is WO 01/21012 PCT/AUOO/01113 3 higher than levels found in other commercial cereal grains. Lipases catalyse the hydrolysis of triglycerides to free fatty acids which cause the oat products to taste rancid and be unpalatable. Whole oats or cut oats that have been kilned may still retain a low level of their original enzyme activity. The 5 remaining enzymes have been found to be inactivated by steaming before the groat is rolled into flakes. Oat flakes or kilned groats may be used to produce commercial oat bran. Most prior art processes for producing cereal bran enriched with p glucan or in processes used to extract p-glucan, generally begin with a step of 10 defatting the groat to avoid rancidity of the final product and to allow for easy separation of the endosperm from the oat bran. Some prior art processes also remove the fibrous material during the process to stabilise the product. The resulting products from most prior art processes are, therefore, devoid of or low in essential fats such as monounsaturated and polyunsaturated fats 15 and/or fibre. Oats have a strong reputation as a nutritious cereal providing more protein than any other cereal as well as insoluble fiber. soluble fiber p glucan. minerals. vitamins, other phytochemicals and the unsaturated fatty acids, oleic and linoleic. Interest in soluble fibers within oats has been 20 spurred by the approval of approved health claims. The benefits for lipid lowering with oats and oat based products have been reported in animal and human studies. Oat gum soluble fiber has been reported to exert a greater hypocholesterolemic effect than several other fibers tested and found to be similar to that of cholestvramine. In humans, 25 the precise effects have been difficult to determine due to the variety of dosages. differing population groups and the nature of the study supplements. Soluble dietary fiber from oats has improved lipidemia, reported in metabolic ward studies and in free-living hyperlipidemic people. Other studies have reported no changes in lipids. In positive trials of oat 30 bran intakes varying between 25 and 106 g daily have been shown to significantly lower serum cholesterol by 5.4 and 12.8% and LDL by 8.5 and 12.4% in moderately hypercholesterolemic subjects. Studies of free living individuals LDL-lowering has been observed with consumption of 88 and 123 g of oat bran respectively. Larger reductions have 35 been reported whereas other well executed trials have proven to be negative.

WO 01/21012 PCT/AUOO/01113 4 There is a need for improved cereal products that retain more of the natural nutritional components and being useful as a food or food supplement. 5 Disclosure of the Invention The present inventors have determined that a bran product, which is enriched with at least two components, selected from P-glucan. fats and fibre derived from the original cereal may be provided through an improved method of processing bran from cereals. 10 In a first aspect, the present invention relates to a method of manufacturing a bran product derived from a grain, the method including the steps of: (a) providing a dehulled grain; (b) stabilising the dehulled grain; 15 (c) pressing or rolling the stabilised dehulled grain to a thickness in a manner that allows minimal disruption to an outer bran layer thereof; (d) milling the pressed or rolled dehulled grain to produce a first grain flour and a coarse grain bran in a manner that allows minimal disruption to the outer bran laver; and 20 (e) sieving and separating the first grain flour from the coarse grain bran; so as to provide the coarse grain bran enriched with at least two components selected from the group consisting of P-glucan. fats and fibre each of which being derived from the grain. In a second aspect. the present invention relates to a method of 25 manufacturing a bran product derived from a grain, the method comprising the steps of: (a) stabilising the grain: (b) dehulling the stabilised grain; (c) separating the stabilised dehulled grain; 30 (d) pressing or rolling the stabilised dehulled grain to a thickness in a manner that allows minimal disruption to an outer bran layer thereof; (e) milling the pressed or rolled dehulled grain to produce a first grain flour and a coarse grain bran in a manner that allows minimal disruption to the outer bran laver: and WO 01/21012 PCT/AUOO/01113 5 (f) sieving and separating the first grain flour from the coarse grain bran; so as to provide the coarse grain bran enriched with at least two components selected from f-glucan. fats and fibre each of which being derived from the grain. 5 In a preferred form, the bran product is enriched with all three components P-glucan. fats and fibre. Preferably. the p-glucan is present in an amount of greater than 10% (w/w): fats in an amount of greater than 10% (w/w); and fibre in an amount of greater than 20% (w/w). Proteinaceous material may also be present in an 10 amount of greater than 15% (w/w). A variety of grains may be used in the methods according to the present method, such as oats, barley, wheat, rice, corn, rye, maize, or millet. While the majority of grains have a hull, there are some varieties of grains that do not have hulls in their natural state. It is also envisaged that the 15 above methods will be applicable to legumes, pulses and oilseeds. The bran from these grains may include germ. The dehulling step may be carried out before or after the grain has been stabilised. Dehulling may be by carried out according to any known prior art method. Preferably, cleaned grains are dehulled prior to 20 stabilisation by impact/abrasion hullers. It will be appreciated that the dehulling step is not required for grains that do not have hulls in their natural state. In prior art process, grains are usually defatted to avoid rancidity in the final products and to ease the separation of the starchy endosperm from the 25 bran. Fat is complexed with starch in the endosperm layers which form part of the bran and it is believed that the disruption of this complex results in the availability of the fat for oxidative rancidity. Further. it is thought that the combination of fat and moisture in the grain make it difficult to separate the cell wall of the bran from the endosperm and by removing the fat, this 30 problem is overcome. In the methods of the present invention, the grain is not defatted. A grain bran with an increased concentration of fats derived from the grain may. therefore, be obtained according to the methods of the present invention. such that the grain bran is shelf stable and not susceptible to 35 rancidity. Such a grain bran has the nutritional advantages of the fats originally contained in the grain. The present inventors have manipulated WO 01/21012 PCT/AUOO/01113 6 the method of producing bran so that the intermediates and products are stable during the stages of the method. The present inventors have recognised that the method requires minimal disruption of the cell walls of the bran (aleurone and endosperm cell layers) as it is separated from the 5 endosperm, that is, minimal disruption to the outer bran layer. This has been achieved with controlled rolling and milling steps, which are discussed below. The grains are stabilised to inactivate the majority of the enzymes in the grain, such as lipases. P-glucanases and amylases and other unwanted 10 enzymes. which may result in the destruction of nutrients in the grain. The stabilisation process also appears to have a beneficial effect on the development of flavour and on resistance to oxidative rancidity. Stabilisation may be carried out by treatment with acids or, preferably, by the known method of kilning. The kilning method preferably involves heating 15 the grain with wet steam for a period to inactivate the enzymes, followed by a holding step for flavour enhancement, followed by a cooling step. Preferably, grain that needs to be dehulled is dehulled prior to stabilisation. Preferably, a dehulled grain is wet steamed at a temperature of 75 to 150 0 C. preferably 95 to 100 0 C. for a period of 15 to 50 minutes, 20 preferably, 20 to 35 minutes. The holding step may be carried out at 130 to 70'C. preferably. 95 to 80'C for a period of 30 minutes to 3 hours, preferably, 1 to 2 hours. The cooling step may be carried out from 130 to 20 0 C preferably. 80 to 30 0 C. for a period of 15 minutes to 2 hours, preferably 30 to 60 minutes. 25 Heat treatment is preferably carried out for a time sufficient to deactivate the majority of the enzymes. Preferably, 100% lipase deactivation (negative result to peroxidase test ) is achieved. The heat treatment step may also be carried out for a time sufficient to control the moisture content of the grain. A relatively high moisture content is desirable. Preferably, a moisture 30 content of 6 % to 16%. more preferably, 9% to 12% is achieved. A high moisture content is preferable in order to compensate the moisture lost during the process and to help make the cell wall of the outer bran more pliable and less likely to be disrupted during the process of removing the endosperm from the outer bran layer. 35 A second heat treatment step may be carried out after step (b) and before step (c) in the first aspect of the invention and after step (a) and before WO 01/21012 PCT/AUOO/01113 7 step (d) in the second aspect of the invention. This may be achieved by steaming, at 80 to 100C, preferably 95 to 90 0 C, over a period of 10 to 60 minutes, preferably 15 to 25 minutes. Preferably, the second heat treatment step is carried out for a time sufficient to inactivate any residual enzymes 5 that were not destroyed during stabilisation. It is believed that the second heat treatment step helps release the endosperm from the cell wall of the bran with minimal disruption. Preferably, the dehulled grain is then subject to milling. As previously stated. the present inventors have recognised that 10 minimal disruption of the outer bran layer of the grain is required to maximise the yield of the P-glucan. fibre and fat components of the bran. The present inventors have achieved this by minimal or no bumping of the grain,. that is. the grain is rolled so that there is minimal or no flattening of the grain. Preferably. the grain is rolled to a thickness of not less than about 15 900 to 1500 ptm. Preferably, the grain is rolled to about 1000 to 1100 pm. This is in contrast to most prior art processes in which the grain is typically rolled to 750 to 550 pm. Milling and sieving of the dehulled grain is carried out to separate grain bran from the first pass of grain flour containing starch. Again, these 20 steps are carried out preferably with minimal disruption to the outer bran layer. Milling may be carried out using a gristing mill or break rolls although it will be appreciated that other similar techniques may be used. Preferably, the gristing mill or break rolls have a flute specification of 10 - 36 flutes/inch D/D. more preferably 28 flutes/inch D/D. Sieving is preferably carried out 25 using a method that allows a dehulled stabilised grain with a relatively high fat and moisture content to be separated from a first pass of grain flour without substantial hindrance. such as clogging of the sieve. Sieving preferably takes place in a vibrosifter. Preferably the grain is sieved through a screen having pores greater than 350 pm, preferably about 350 to 600 pLm 30 and more preferably, about 400 to 500 pm. This step produces coarse bran, preferably as flakes and more preferably, with a diameter of approximately about 2 to 5 mm diameter. For example. in the production of course oat bran, >90% of the bran stayed on a 500 pm screen (see Table 3). These flakes have some adherence of endosperm. 35 The coarse grain bran may undergo a succession of grinding and sieving steps to separate flour from the grain bran to produce a grain bran WO 01/21012 PCT/AUOO/01113 8 product of varying density and particle size. Once again, these steps are preferably carried out in a manner such that there is minimal disruption to the outer bran layer until the endosperm is mostly removed. All grinding steps are preferably carried out at low speeds and sieving is preferably 5 carried out utilising density rather than particle size to minimise disruption to the outer bran and to facilitate removal of the endosperm. Preferably. the grinding step is followed by an air density separation step. Sieving may be achieved by known processes such as air classification or vibratory sifters or the like. More preferably. the air classification system is used. This system 10 preferably consists of an attrition type ring grinder, the output particle size of which is controlled by balancing the centrifugal force applied by the fixed speed rotor, with the air flow through the grinder as regulated by the variable speed classifier within. After grinding, two components may then be separated using a main classifier, which separates on the basis of density in a 15 moving variable air stream. The coarse grain bran may undergo a grinding step followed by a sieving step to separate fine grain bran from a second pass flour. Preferably, the air speed setting for air travelling through the grinder is about 125 to 1000 rpm, more preferably. about 250 to 650 rpm. Preferably, the coarse bran is 20 passed through an air classifier to remove a second pass of grain flour, the air classifier. preferably has a setting of about 75 to 475 rpm. more preferably, about 150 to 350 rpm. The resulting fine grain bran preferably has an average diameter of approximately about 100 to 1000 pm. For example, in the production of fine oat bran, > 70% of the bran passed through 500 ptm 25 screen and remained on a 250 pm screen (see Table 3). The fine grain bran may undergo a further grinding step and sieving step to separate a grain bran product that will hereinafter be referred to as the coarse grain bran concentrate, from a third pass of grain flour. Preferably, the air speed setting for air travelling through the grinder is about 200 to 1200 30 rpm, more preferably, about 400 to 800 rpm. Sieving may be achieved by known processes such as air classification, rotary sifters or the like. Preferably, the coarse bran is passed through an air classifier to remove a third pass of grain flour, the air classifier, preferably has a setting of about 75 to 600 rpm. more preferably. about 150 to 400 rpm. The resulting coarse 35 grain bran concentrate preferably has an average diameter of about 50 to 300 pm. For example. in the production of coarse oat bran concentrate, > 70% of WO 01/21012 PCT/AUOO/01113 9 the bran passed through 425 pm screen and remained on 150 ptm screen (see table 3). The coarse grain bran concentrate may undergo a further grinding step to produce a superfine grain bran concentrate. A further sieving step may be 5 carried out to separate a fine grain bran concentrate from a fourth pass of grain flour. Preferably, the air speed setting for air travelling through the grinder is about 400 to 2000 rpm, more preferably, about 600 to 1000 rpm. Sieving may be achieved by known processes such as air classification. Preferably. the ground coarse bran concentrate is passed through an air 10 classifier to remove a fourth pass of grain flour, the air classifier, preferably has a setting of about 100 to 500 rpm. more preferably. about 150 to 300 rpm. The resulting fine grain bran concentrate has an average diameter of about 20 to 150 jam. In the production of fine oat bran concentrate, > 70% of the bran passed through 250 pam screen (see Table 3). 15 In a more preferred embodiment, the present invention is directed to a method of manufacturing an improved bran product derived from a grain, the method comprising the steps of: (a) providing a dehulling grain: (b) stabilising the dehulled grain: 20 (c) subjecting the stabilised dehulled grain to a heat treatment step; (d) pressing or rolling the heat treated dehulled grain to a thickness in a manner that allows minimal disruption to an outer bran layer; (e) milling the pressed or rolled dehulled grain to produce a first grain flour and a coarse grain bran; 25 (f) sieving and separating the first grain flour from the coarse grain bran (g) grinding the coarse grain bran to produce a second pass flour and a fine grain bran: (h) sieving and separating the second pass flour from the fine grain bran; (i) grinding the fine grain bran to produce a third pass of grain flour and a 30 coarse grain bran concentrate; (j) sieving and separating the third pass of grain flour from the coarse grain bran concentrate: followed by either (k) grinding the coarse grain bran concentrate to produce a superfine grain bran concentrate: or 35 (1) grinding the coarse grain bran concentrate to produce a fourth pass of grain flour and a fine grain bran concentrate: WO 01/21012 PCT/AUOO/01113 10 (m) sieving and separating the fourth pass of flour from the superfine grain bran concentrate; to produce a coarse fine or superfine grain bran concentrate enriched with at least two components selected from p-glucan. lipids and fibre being derived 5 from the grain. In a preferred form, the bran product is enriched with all three components p-glucan. fats and fibre. Preferably, the P-glucan is present in an amount of greater than 10% (w/w): fats in an amount of greater than 10% (w/w); and fibre in an amount 10 of greater than 20% (w/w). Proteinaceous material may also be present in an amount of greater than 15% (w/w). Preferably. the invention according to the first or second aspect is carried out using oats. In a further preferred embodiment, the present invention relates to a 15 method of manufacturing an improved oat bran product derived from oat, the method comprising the steps of: (a) dehulling the oat (b) separating the dehulled oat; (c) subjecting the dehulled oat with a first heat treatment step; 20 (d) stabilising the first heat treated dehulled oat to a second heat treatment step: (e) rolling the second heat treated dehulled oat to a thickness in a manner that minimises disruption to an outer bran layer; (f) milling the oat to produce a first grain flour and a coarse oat bran; 25 (g) sieving and separating the first grain flour from the coarse oat bran: (h) grinding the coarse oat bran to produce a second pass of oat flour and a fine oat bran: (i) sieving and separating the second pass of oat flour from the fine oat bran: 30 (j) grinding the fine oat bran to produce a coarse oat bran concentrate and a third pass of oat flour: (k) sieving and separating the coarse oat bran concentrate from the third pass of oat flour (1) grinding the coarse oat bran concentrate to produce a superfine bran 35 concentrate: or WO 01/21012 PCT/AUOO/01113 11 (m) grinding the coarse oat bran concentrate to produce a fourth pass of oat flour and a fine oat bran concentrate; (n) sieving and separating the fourth pass of oat flour from the fine oat bran concentrate; 5 to produce a coarse, fine or superfine oat bran concentrate enriched with at least two components selected from p-glucan, lipids and fibre being derived from the oat. Preferably. the improved oat bran product in the form of the oat bran concentrate product is enriched with at least two of the following: 10 (a) greater than 10% (w/w) P-glucan; (b) greater than 10% (w/w) fat: and (c) greater than 20% (w/w) total dietary fibre (TDF); wherein the p-glucan, fat and TDF are derived from the oat. In a preferred form, the oat bran product is enriched with all three 15 components P-glucan, fats and fibre. Proteinaceous material may also be present in an amount of greater than 15% (w/w). The improved oat bran product may be used to produce oat bran enhanced food products such as bread, biscuits, cereal flakes, muesli bars, 20 extruded snacks and as a supplement for drinks. In a third aspect. the present invention is directed to grain brans and bran concentrates produced according to the first or second aspects of the present invention. In a fourth aspect, the present invention is directed to the use of grain 25 brans and grain bran concentrates obtained by the process according to the first or second aspect of the invention in the manufacture of food and/or drink products. The resulting grain brans and grain bran concentrates may be used in the preparation of food and drink products. For instance, they may be used 30 in the preparation of bakery products such as bread, biscuits and pastry; cereal products such as cereal flakes, muesli bars: as a powder supplement in drinks: meat and meat analog products: soups, sauces, pastes: confectionary products: butters: spreads; non-frozen and frozen desserts; dairy and dairy analogs: dietary supplements: cosmetics: stockfeed animal and veterinary 35 care products. The resulting food and drink products are nutritionally WO 01/21012 PCT/AUOO/01113 12 enriched with the grain bran and/or grain bran concentrate produced according to the first or second aspects of the invention. In a fifth aspect, the present invention is directed to a grain flour prepared according to the method according to the first or second aspect of 5 the invention. It will be appreciated that the grain flour may contain proteins, -glucan and fats which may be fractionated by known methods and further processed for use in food and drink products. In a sixth aspect. the present invention is directed to a method of producing a bran gel or paste hereinafter referred to as a bran gel, the method 10 comprising subjecting the grain bran or grain bran concentrate made according to the first or second aspect of the present invention to enzymatic starch hydrolysis. The coarse or fine grain bran or the grain bran concentrate in either, coarse. fine or superfine forms may undergo further processing steps to 15 produce a bran gel. More preferably. the grain bran concentrate is subject to enzymic starch hydrolysis to produce a bran gel, being an enzymically modified grain bran concentrate. In a preferred embodiment, the present invention is directed to a method of producing bran gel, the method comprising treating an aqueous 20 solution of the grain bran concentrate product with an c-amylase under conditions which will hvdrolyse the grain bran concentrate product to produce an bran gel. Preferably, an aqueous suspension of the grain bran concentrate, a amylase and optionally p-amylase is heated to a temperature within the 25 operable range 50 to 95'C. preferably 70 to 90 0 C. More preferably, the grain bran concentrate contains 16 to 70%. more preferably 35 to 45% solids. Preferably. the pH is maintained between 5.0 to 8.0. more preferably. 6.0 to 7.0. The mixture may be cooled to less than approximately 70 0 C. preferably less than 50 0 C. after approximately, 10 to 120 minutes and the pH is 30 preferably adjusted to 4.5 for approximately 10 to 30 minutes after which the pH is preferably adjusted to approximately 6.5. This step functions to deactivate the enzyme and it will be appreciated that other methods known in the art may be used eg addition of chelating agents such as poly phosphate or heat treatment such as UHT for approximately 4 secs at approximately 35 121'C. The final product may be pasteurised or sterilised and packed. Alternatively. the final product may be dehydrated by known means to WO 01/21012 PCT/AUOO/01113 13 produce a bran gel powder. The powder is suitable for reconstitution or rehydration into a liquid preparation. The powder may also be placed in a water limited environment, for example in a lipid based or cheese spread. The bran gel powder may be used in the production of drinks, ice 5 cream, dairy and dairy analogs, frozen and non-frozen desserts, meat and meat analogs, soup, sauces, pastes, dressings, spreads or as a fat replacer. The bran gel powder may also be used in stabilising syrup systems such as in food coating and binding such as syrup for muesli bars. The grain brans, grain bran concentrate products and grain bran gels 10 may be added to any number of food products and combined with any number of dietary supplements such as vitamins and minerals. Preferably, oat bran or an oat bran concentrate in either, coarse, fine or superfine forms undergoes further processing to produce an oat gel in the above described manner. 15 Oat gel has a nutritional profile equal to or better than traditional wet oat products such as, porridge but in a form that has broader organoleptic appeal and more readily consumable form. The oat gel is capable of being added to other products to enable delivery of the goodness of oats in a broader product format. By further processing the oat bran concentrate, it is 20 converted into a form that can be readily consumed as nutritious gel, delivering the enriched goodness of oats. The gel may be also added to other foods to fortify them with the nutritional benefits of oats. For instance, the oat gel powder can be used in the production of the food and drinks listed above and in stabilising syrup systems. 25 The oat bran and oat bran concentrate products according to the invention generally contain residual starch. The difference between the oat bran/oat bran concentrate and the oat gel powder is that the residual starch present in the oat bran/oat bran concentrate is converted into a form that does not interfere with liquid processes. The formation of the oat gel powder 30 employs the use of an enzyme that breaks down the starch and therefore reduces the viscosity of the oat gel powder. In a seventh aspect, the present invention consists in an improved grain bran product derived from grains, the grain bran product comprising at least two of the following components: 35 (a) greater than 10% (w/w) p-glucan; (b) greater than 10% (w/w) lipid; and WO 01/21012 PCT/AUOO/01113 14 (c) greater than 20% (w/w) total dietary fibre (TDF); wherein the p-glucan, lipid and TDF are derived from the grains used to make the grain bran product. Proteinaceous material may also be present in an amount of greater 5 than 15% (w/w). The grain bran product is not supplemented with additional quantities of components (a) to (c), all components are derived from the grains used to produce the grain bran. The grain bran product is shelf stable and despite the high lipid concentration does not go rancid under normal handling 10 conditions. Preferably, P-glucan is present in an amount of 10 to 20% (w/w), lipid in an amount of 10 to 20% (w/w) and TDF in an amount of 23 to 50% (w/w). The TDF is defined as containing non-starch polysaccharides (NSP), resistant starch (RS) and lignin and may be classified into soluble and non-soluble 15 fibre. Preferably, the soluble fibre is in an amount of 6 to 30% (w/w) and the insoluble fibre in 15 to 50% (w/w). The grain bran product may also include proteinaceous material in an amount of 15-50% (w/w), ash in an amount of 3 to 10% (w/w), carbohydrate in an amount of 30 to 70% and starch in an amount of 25 to 55% (w/w). The 20 grain bran product may further include vitamins such as thiamine, riboflavin, niacin. alpha-tocopherol. pyridoxine and folate and minerals such as calcium, iron. potassium. magnesium, sodium, phosphorous and zinc. In a further preferred embodiment. the present invention provides an improved oat bran product derived from oats. 25 In a preferred embodiment. the oat bran product comprises: (a) 10-18% (w/w) p-glucan: (b) 12 to 15% (w/w) lipid: (c) 17 to 19% (w/w) insoluble fibre: (d) 10 to 25% (w/w) soluble fibre: 30 (e) 18 to 26% (w/w) proteinaceous material; (f) 50 to 60% (w/w) carbohydrate; and (g) 4 to 5% ash (w/w); wherein the 0-glucan. fat and TDF are derived from the oats used to make the oat bran product. 35 Throughout this specification. unless the context requires otherwise, the word "comprise". or variations such as "comprises" or "comprising", will WO 01/21012 PCT/AUOO/01113 15 be understood to imply the inclusion of a stated element, integer or step, or group of elements, integers or steps, but not the exclusion of any other element, integer or step, or group of elements, integers or steps. Any description of prior art documents herein is not an admission that 5 the documents form part of the common general knowledge of the relevant art in Australia. In order that the present invention may be more clearly understood preferred forms will be described with reference to the following examples and drawings. 10 Brief Description of Drawings Figure 1 shows a flow diagram for the steps used to produce the oat bran and oat Bran concentrate products according to a preferred embodiment of the invention. 15 Figure 2 shows the viscosity of a number of oat bran products including an oat bran product according to the present invention. Modes for Carrying out the Invention METHODS 20 Preparation of stabilised groat (KDHO) - Cleaned oats were passed through an impact and abrasion hullers and - Then through a clipper to remove double hulls and tricomb hairs - The mixture is then aspirated to remove hulls. - Green groats are then steamed with live steam to a temperature of 95 25 100 0 C. 30 60 minutes - Flavour development is then carried out through kilning the groat by holding it a 90-70'C for 1-2hrs. -At which time the groats are air cooled to 1 hour 30-40 0 C. The moisture of the oat started at about 9-10% mc and while its moisture 30 increased in the steaming step, the cooling step counters it so that the final moisture was the same as it started.

WO 01/21012 PCT/AUOO/01113 16 Preparation of rolled oats - The stabilised groat was steamed for 10-25 mins, which brings the temperature to 95-90 0 C. - The groat was then fed into the flaking mill to produce a rolled oat. 5 For this process the preferred thickness, targeted, is 1050 pm. - The rolled oat was then cooled. Preparation of coarse oat bran - The rolled oat was then fed into a gristing mill (28 flutes/inch D/D). 10 Alternatelv a breaker mill could be used. - The material was then passed through a rotary sifter with a 450 ptm nylon screen, with the overtails material being described as a coarse oat bran. Approximately -50/50 yield of bran to flour was obtained. The analysis of the coarse oat bran obtained is indicated in Table 1. 15 Preparation of fine oat bran - Coarse oat bran was feed into grinder/classifier 1 speed setting 400rpm. - Then into Classifier 2 speed setting 290 rpm 20 The low-density fraction collected was Fine oat bran. Yield -70/30 bran: flour. Preparation of coarse Oat Bran concentrate - Fine oat bran was repassed through fine grinder/ classifier 1 speed 25 setting 590 rpm. - Then through Classifier 2 speed setting 290. The low-density fraction was coarse Oat Bran concentrate. Yield -60/40 bran:flour. 30 Preparation of oat Bran concentrate fine - Coarse Oat Bran concentrate was repassed through the fine grinder/classifier 1 speed setting 800 - Then through Classifier 2 speed setting 250 The low-density fraction was fine Oat Bran concentrate. Yield - 45/55 35 bran:flour WO 01/21012 PCT/AUOO/01113 17 Preparation of oat gel RECIPE: 200 kg water 50 kg Oat bran 0.15 kg a-amvlase PROCEDURE: 1. Add to tank and preheat water to 80'C. 2. Turn on mixer simultaneously add enzyme and oat bran 3. Hold at 80'C for one hour. 4. Cool product to 50"C. 5. Adjust to pH 4.5 with dilute HCl and hold for 15 min to deactivate enzyme. 6.. Adjust to pH 6.5 with dilute NaOH 7. Fill and pack. EXAMPLES 5 The following Examples demonstrate the use of the oat bran concentrate product according to the present invention in the manufacture of bakery products. Example 1- Extruded (Processed) Bran Formulation Including Oat Bran 10 concentrate Ingredient Kg Oat Bran concentrate 150.0 Fine Bran 30.0 Sugar 25.0 Natures Gold Flour 30.0 Liquid Malt 30.0 Water 40.0 Total 295.0 Method: 1. Blend Dry ingredients to achieve a homogenous mix. 2. Feed blended dry ingredients into extruder dry feed system. 15 3. Feed water and malt into the barrel of the extruder and introduce dry feed. 4. Extrude product: Screw speed = 750 WO 01/21012 PCT/AUOO/01113 18 Feed rate = 475 kg/hr Water rate = 78 L/hr Malt feed rate = 45 kg/hr Barrel Temperature = 100 0 C 5 5. Cut extruded product and dry through an oven at 165 0 C Example 2 - Flake Formulation Including Oat Bran concentrate Ingredient Kg Oat Bran concentrate 250.0 Wheat 125.0 Oats 250.0 Rice 75.0 Honey 35.0 Liquid Sugar 80.0 Water 200.0 Condensate 80 Total 1095.0 10 Method: 1. Blend Dry ingredients to achieve a homogenous mix. 2. Make up a syrup premix with the wet ingredients. 3. Load both the syrup premix and the dry premix into the rotary cooker. 4. Cook the raw materials in the cookers at a pressure of 140 kPa, for 22 15 minutes plus a ramp up and ramp down time. 5. When cooked. form product into pellets and dry through an oven. 6. Run pellets through flaking mills. 7. Toast flakes and allow to cool.

WO 01/21012 PCT/AUOO/01113 19 Example 3: Bread Recipe using Oat Bran concentrate Bread Recipe Formulation Bakers Flour 750g Oat bran concentrate 250g Gluten 50g Salt 20g Improver log Yeast 40g Water 730g 5 Method: Made as per rapid bread making method commonly known in the art. Example 4: Anzac Biscuit Recipe using Oat Bran concentrate Anzac Biscuit Recipe Formulation Shortening 125g Golden syrup 1 tablespoon Boiling water 2 tablespoons Bicarbonate of soda 1.5 teaspoons Rolled oats 1 cup Oat Bran concentrate 0.75 cup Desiccated coconut 0.5 cup Plain Flour 0.5 cup Sugar 1 cup 10 Method: Blend dry ingredients Blend melted shortening, golden syrup. boiling water and bicarbonate.

WO 01/21012 PCT/AUOO/01113 20 Combine wet and dry ingredients. mix Dispense controlled portion onto greased oven trays Bake 150 0 C for 20 minutes 5 Table 1: Typical Nutrient Analyses of Coarse Oat bran concentrate (a) Macronutrients % % (dwb) moisture 7.2 0.0 protein (N x 6.25) 22 23.7 fats (ee) 12.1 13.0 Fats (AH) 14.5 total carbohydrate 54.5 58.6 total sugars 2.6 2.8 TDF 28.4 30.6 insoluble fibre 17.5 18.9 soluble fibre 10.9 11.7 ash 4.3 4.6 beta-glucan 12.4 13.4 total starch 0.0 Yield <24 <24 (b) Micro-nutrients vitamins % minerals mg/100g thiamin 0.56 calcium 120 riboflavin 0.13 iron 5.9 niacin 1.7 potassium 770 beta-carotene - magnesium 390 alpha-tocopherol 0.6 sodium 2.2 Alpha-tocopherol aqu 0.82 phosphorous 1100 pyridoxine 0.12 zinc 6.6 free folate total folate 62.8 dwb = dry weight base 10 ee = ether extraction as per AOAC method 920.39C AH = acid hydrolysis as per AOAC method 922.06 WO 01/21012 PCT/AUOO/01113 21 CC~Z _- cq L Nv L n _ co ~ U ~ .C -rA NmC u Ntz Cj co Lo 06 t rZLr oc -. nc m ccl~J . . . o co como__ .0 L co m co 0 )c'i LO c. Ln ~ (0 m CZ m~ cc m 0Z m CC L (0~ O~ C' 0 - NC~ N CNIW C) N ( 0; UC, a)o)a x CCT CC ;a CD_ -S - - cn : I= Ln_ 0 WO 01/21012 PCT/AUOO/01 113 22 0w C) Lw coco coC) o -o Nw cor Lw co ,I C)C4 Lrw CA oCL c= CD o -~ C) c) C) Cl " WO 01/21012 PCT/AUOO/01113 23 Over 100 tonne of bran products have been produced in accordance with the present invention. The bran products according to the present invention have been used in a variety of food products and that use has not compromised the taste,. texture or shelf life of any of the products. This is in 5 contrast to many of the prior art bran products which can become rancid due to the fat component. Not only does the present invention provide an important dietary component or additive for food products, it does not adversely effect the character or quality of the food products. 10 Preparation of enriched p-glucan samples for rheological and molecular weight analysis Samples The following samples were received and analysed: 20476/1 Oat Gold 15 20476/2 H.W. Flakes 20476/3 Premix 20476/4 Bran 20476/5 Bread 20476/6 Muffins 20 20476/7 Rolled Oats Sample storage Samples 1-4 and 7 were stored at room temperature. Samples 5 and 6 were stored at -701C. After initial inactivation of endogenous enzymes, samples were stored in a desiccator at room temperature. 25 Sample preparation Mechanical treatment Samples 1 and 3 were used without any grinding. Samples 2, 4 and 7 were ground in a coffee mill. Sample 5 was frozen in liquid nitrogen and then ground with a mortar and pestle. Sample 6 was thawed, broken into 30 small pieces. re-frozen with liquid nitrogen and then ground with a mortar and pestle Inactivation of endogenous enzymes Ten grams of each sample (samples were not dried) were boiled in 100 ml 90% ethanol under reflux for 20-30 min. After cooling, the supernatant 35 was decanted, and the residue was washed in 50 ml 98% ethanol. After settling. the supernatant was again decanted and the residue was dried - WO 01/21012 PCT/AUOO/01113 24 initially in air and then in vacuum. Residual moisture was removed by freezing at -70 0 C and freeze-drying. Extraction of soluble P-glucans and de-starching Duplicate preparation of each sample was carried out. Two grams of 5 each sample were weighed into 50 ml Falcon tubes and suspended in 50 ml of 50 mM sodium phosphate buffer pH 6.5 containing 80 ppm calcium chloride. The tubes were then placed into a water bath preheated to 90'C. Tubes were initially shaken every -5 min. After 20 min, 500 sl of Thermamyl 120L type LS (Novo Nordisk, Denmark) was added. Two controls 10 which did not receive any thermamyl were also extracted. Extraction and digestion continued for a further 2 hours with shaking of the tubes every 15 20 min. The tubes were then centrifuged in a table top centrifuge at -2.400g for 10 min. The residue was re-extracted with 10 ml cold buffer, re centrifuged and the supernatants combined. The extracts were checked for 15 the presence of starch by iodine/iodide analysis. Samples were then centrifuged at -35.000g for 1 hour. The resulting supernatants were decanted through mira-cloth. and p-glucans were then precipitated with 2 vol ethanol. The precipitated samples were allowed to stand for at least 2 hours at room temperature before gentle centrifugation at -500g for 10 minutes to 20 pellet precipitated P-glucan (coherent "mats" or "globs" of precipitate were removed before centrifugation and combined with the pelleted material after centrifugation). The precipitate was washed first with 50 ml 66% ethanol and then with 15 ml pure ethanol. After initial air-drying. the samples were frozen and freeze-dried. The weight of each sample was recorded after 25 freeze-drying.

WO 01/21012 PCT/AUOO/01113 25 Results No starch was detected in any sample. Starch was abundant in controls that were not treated with Thermamyl. 5 Table 4: Yield after inactivation with ethanol and subsequent drying Sample Weight (g) Yield (%) 1 8.1 81 2 8.1 81 3 7.1 71 4 7.8 78 5 5.3 53 6 4.1 41 7 8.0 80 Table 5. Yield after extraction and drying Sample Weight (g)' Average Yield Fraction of (%)2 original sample (%)3 1 0.22 ; 0.22 11 8.9 2 0.13 :0.13 6.5 5.3 3 0.19 :0.19 9.5 6.7 4 0.16 :0.15 7.8 6.1 5 0.09 :0.05 3.5 1.9 6 0.13 0.10 5.8 2.4 7 0.11 :0.10 5.3 4.2 10 These are the weights of the duplicate samples after freeze-drying. Please note that this is the recorded weight of the total ethanol precipitate. These precipitates may contain small amounts of protein and other non starch polysaccharides. 15 2 This was the yield of precipitate as a percentage of the amount of sample used for extraction after pre-treatment in ethanol and drying. 3 This was the yield of precipitate as a percentage of the original sample.

WO 01/21012 PCT/AU0O/01113 26 Comments to preparation of p-glucan enriched samples The yield of -glucan extraction is very dependent on extraction conditions, especially temperature (Beer et al 1997 Cereal Chem. 74; 705 709). An extraction procedure was chosen that would extract the majority of 5 the extractable P-glucan. This procedure was essentially based on the "hot water" method described by Beer et al (1997). However, to increase the purity of the samples, -glucan was precipitated with ethanol prior to quantification and analysis (Doublier and Wood 1995 Cereal Chem. 72; 335 340: Johansson et al 2000 Carbohydr. Polym. 42; 143-148: Wikstr6m et al 10 1994 J. Food Sci. 59: 1077-1080; Wood et al 1994 Cereal Chem. 71; 301-307). It should be noted that the p-glucan preparations might contain small amounts of protein and other non-starch polysaccharides such as xyloglucans (Miller and Fulcher 1995 Cereal Chem 72; 428-432). A complete purification and quantification was not performed as the main purpose of the analysis 15 was to detect changes in molecular weight of the P-glucans as a function of processing conditions of the food samples. Characterisation of p-glucan from oats and products The following freeze-dried samples labelled 1-7 and A-F (duplicates of 20 1-7) were measured for shear viscosity, intrinsic viscosity and particle sizing. Methods Shear viscosity Measurement The freeze-dried samples were dispersed in distilled water and left to hydrate at room temperature for 2 hours. The dispersion was then heated to 25 70'C in a waterbath and then mixed with a Ystral high speed mixer for 30 seconds, it is then left to hydrate further for an hour at room temperature. A 1.0 % dispersion was used for shear viscosity measurement. Shear viscosity was measured on a 4 cm cone-plate system (59 sm gap, 1.590 angle) with the Carri-Med Controlled Stress Rheometer (CSL 100) at 30 25"C. Viscosity was plotted against shear rate. Intrinsic Viscosity Measurements The freeze-dried samples were dispersed in distilled water and left to hydrate at room temperature for 2 hours. The dispersion was then heated to 70'C in a waterbath and then mixed with a Ystral high speed mixer for 30 35 seconds, it was then left to hydrate further for an hour at room temperature. The dispersion was filtered with a 2 sm filter before measurement.

WO 01/21012 PCT/AUOO/01113 27 The intrinsic viscosity is measured with the Ubbelohde viscometer attached to a Schott AVS 350 and TA20 set-up for automatic dilution. The p glucan concentration was kept to 0.1%. The measurement was conducted in a constant 25 0 C water-bath. 5 Intrinsic viscosity was expressed as ml/g GPC-MALLS Measurements Several attempts to measure the molecular weights using GPC-MALLS. These were unsuccessful as the beta glucan appeared to irreversibly adsorb to the GPC columns. Flushing of the column with water was found to elute a 10 number of peaks. This indicates that the p-glucans are adsorbing to the columns at high salt. Other workers have measured p-glucans in O.1M NaNO 3 as undertaken in this study. The molecular weights measured were of order 1.5M. Dvnamic Light Scattering 15 The solution hydrodynamic size was measured using dynamic light scattering. The sizes reported below are in dilute solution and are a measure of the hydrodynamic sizes of the molecules in free solution. Comparison of these sizes with free dextran of 2M molecular weight yielding a size of 26 nm diameter indicates that the beta glucan exists as a very large molecule in 20 solution or as an aggregated species. The monodispersity of the 70 nm particles and their apparent large percentage of the material indicates that a single type of aggregate structure was present. Either acid or alkali treatment is required to determine the primary molecular size. 25 Table 6. Particle sizes: Diameter Sample D1 (nm) % vol Mwt x 10- % Vol D2 (nm) 1 71 60 38 40 505 2 73 30 36 70 360 3 46 80 23 20 303 4 75 70 37.5 30 552 5 72 50 36 50 650 6 60 70 30 30 380 7 103 30 70 70 600 WO 01/21012 PCT/AUOO/01113 28 Particle sizes indicate that the molecules are aggregated to a significant degree. The primary particle sizes. D1 and D2 vary significantly in their relative percentage by volume. This indicated that significant amounts of aggregated material is present after rehydration using the above procedure. 5 The stated molecular weights (from the smaller diameter) are calculated using dextran as a standard and assuming both polysaccharides are in the random coil state. The molecular weights interpreted from PCS measurement are significantly larger than those measured using GPC-MALLS by other (Cui and Wood 2000 Hydrocolloids - Part 1. Ed. Nishinari, Elsevier, 10 159- 169). The molecular weights determined using GPC-MALLS indicated lower values of order 0.4 through to 3.1M. The shear imposed on the system by the GPC column may be responsible for the reduction in the apparent molecular weights measured. Essentially. the data obtained in this study indicates that the p-glucan is in aggregated form in aqueous solution. The 15 aggregates will therefore be the species which show physiological activity unless the polymer aggregates disperse in the acid conditions of the stomach. Table 7. Viscosity and particle size of sample dispersion Sample Shear Intrinsic Powder/Solution viscosity viscosity characteristics (Poise, 100/s) (ml/g) 1 3.00 643 Fluffy white/clear 2 0.55 369 Hard Brittle/clear 3 0.55 415 Hard Brittle/clear 4 0.20 296 Hard brittle/clear 5 0.06 235 vellowish/clear 6 0.90 428 Fluffy white/clear 7 0.27 381 Brownish/Cloudy 20 WO 01/21012 PCT/AUOO/01113 29 Table 8. Duplicate samples Sample Intrinsic viscosity ml/g 1 623 2 423 3 449 4 290 5 259 6 460 7 502 Discussion 5 Intrinsic viscosity was used as a measure of duplication of the extraction process and Tables 7 and 8 clearly indicates that there was reproducibility to +/- 10 mL/g. Shear viscosity and intrinsic viscosity show similar trends. The shear viscosity can potentially be used as a quick measurement to determine 10 degradation of samples. Shear viscosity is usually less time consuming to perform and in factory environment is often the only available method for measurement of viscosity. Figure 2 shows the viscosity curves of the samples 1-6. Interestingly, there appears to be little correlation between the 15 hydrodynamic sizes measured using light scattering and the intrinsic viscosity measurements. Both measurements are of the dilute solution hydrodynamic size. The linearity of the intrinsic viscosity curves indicates that there is little or no interaction at these concentrations. The differences observed are due to the differences in the relative amounts of the aggregated 20 material. There was a correlation between the relative percentage of the two particle sizes and the measured intrinsic viscosities. Given that the degree of aggregation of the p-glucans is likely to increase in pH 2 solutions due to increased hydrogen bonding. Similar acid conditions are found in the stomach and therefore it is believe that the 25 aggregated material will be that which is found in the physiological conditions.

WO 01/21012 PCT/AUOO/01113 30 Conclusion The p-glucan extracted from the different oat sources all show significant aggregation and are not low molecular weight material. There appeared to be two major species present at approximately 70 nm and 300 5 600 nm diameters as aggregates in solution. Affects on Total Cholesterol by p-glucan product METHOD Experimental design 10 This section describes a single blind study of free living hvperlipidaemic participants testing a novel oat preparation derived predominately from the aleurone layer of oat bran through a new processing technique. The study began with a two-week control phase during which subjects 15 became accustomed to a diet avoiding barley and oat products, specialty margarines and psyllium. Subjects then proceeded to a 3-phase test period comprising low glucan, high glucan and low glucan supplemented foods. Each phase lasted 3 weeks. The design therefore provided two control periods flanking the test period. The habitual diets were determined by a 20 modified food frequency questionnaire. which served to establish for each person a constant background pattern for eating. The p-glucan was incorporated into three foods - cereal, muffins and bread in approximately equal amounts within 40 g breakfast cereal, 70 g sliced bread and a 50 g muffin. The low glucan periods comprised the same 25 study foods but without p-glucan supplementation. The foods were eaten through the day. All foods were colour-coded but only the subjects were blinded to the identity of the foods. The laboratories carrying out the various assays. however. were ignorant of the nature of the studv. 30 The p-glucan was produced according to the present invention. For this study. approximately 60 g of oat bran concentrate contained 8 g p glucan.

WO 01/21012 PCT/AUOO/01113 31 Subjects Fifteen men and women were recruited by advertisement that sought people with known hypercholesterolemia who had not been treated with lipid-lowering drugs. One subject clearly did not comply and was excluded. 5 Inclusion criterion was a total cholesterol >5.5 mmol/L. Exclusion criteria included smoking, alcohol exceeding 2 standard drinks per day, dietary supplements. medication likely to affect plasma lipids, bowel, liver and kidney disorders, thyroid dysfunction and diabetes mellitus. 10 Each subject completed a 3-day food frequency questionnaire modified to focus on fats, cholesterol and fiber at the beginning and end of the study. The Human Ethics Committee of the Alfred Group of Hospitals, Melbourne approved the study. and volunteers gave written consent following full disclosure and explanation of the study. 15 Laboratory Procedures Blood was drawn twice at the beginning and twice at the end of each phase of the study. Values for the sampling were averaged. Plasma was stored at -80 0 C for measurements of cholesterol, triglyceride and HDL 20 cholesterol by standard enzymatic techniques. LDL was calculated from the above. Statistical Analysis The experimental design allowed pairwise comparisons between low 25 and high glucan periods, so that analyses were based on paired t-test. RESULTS The average age of the 7 men and 7 women was 52± 10 yr (range 34-69) and their BMI was 25 3.4 kg/in (range 19.3-29.8) as shown in Table 9. 30 Plasma lipid values on screening showed that two subjects were inadvertently entered with a cholesterol value below 5.5 mmol/L (the inclusion criteria): 5.2 mmol/L, 5.4 mmol/L. They were therefore primarily hypercholesterolemic. All subjects were overweight with BMI greater than 25 cn 2 /kg, none were obese. All men and women had abdominal obesity 35 defined as > 0.9 for men and > 0.8 for women. Glucose readings were within the normal range.

WO 01/21012 PCT/AUOO/01113 32 Dietary record The 3-day food frequency questionnaire showed that the percent energy from fat (35 5 versus 34±6) and from saturated fat (13±4 versus 5 13t4) was not significantly different during the low and high glucan periods. Total fiber from the background diet (18: 5 g) remained constant. The average daily p-glucan consumption was 7.4 t 1.28 g during the supplemented phase, 92% of the 8 g target. Compliance, as judged from the daily records of test foods consumed and the 3-day food frequency 10 questionnaire,. was very satisfactory. The lower than targeted consumption of p-glucan was due to some people,. especially women, unable to eat the full ration of muffins. Lipid changes with low and high P-glucan 15 Plasma cholesterol concentrations fell significantly between low and high 4.4% (P=0.035) and high and low 4.6% (P=0.013) p-glucan diets as reported in Table 10. The effect of the beta glucan supplement was greater for LDL cholesterol: 9.1% (P=0.002) reduction between low and high and 7.7% (P=0.003) between high and low glucan periods. The minor changes in 20 triglyceride. HDL body weight, and BMI were not significant. DISCUSSION This study demonstrated that the major soluble fiber of oats, f-glucan decreased plasma cholesterol and LDL levels in a group of free-living. 25 hypercholesiezalaemic people (Table 10). Whereas the majority of similar trials have reported a reduction of blood cholesterol in hypercholesterolaemic subjects consuming oat bran other studies have found no significant effect. Several factors might account for this variability in studies: (i) large quantities of oat bran may lead to other specified dietary 30 modifications (ii) low B-glucan content of bran. (iii) different responses among normocholesterolaemic and hypercholesterolaemic subjects. In this study, a well-defined oat bran concentrate providing 8 g p glucan was added to a variety of foods. All subjects had plasma lipid values in excess of 5.2 mmol/L and dietary records showed similar intakes of other 35 key nutrients such as saturated fat throughout.

WO 01/21012 PCT/AUOO/01113 33 The average cholesterol lowering of 4% and LDL lowering of 9% is in line with results reported by others who used 95 g oat bran concentrate or 57 g of dry instant oats. There has been a reported almost twice the effect of the present study (9% vs 4.4% for serum cholesterol and 10% vs 9.1% LDL 5 lowering) in subjects with similar baseline cholesterol levels (6.76 t 0.13 vs outs 6.4 ± 0.80 ) utilizing only 3g P-glucan derived from oat bran compared with 8 g p-glucan from oat bran in our study. The meta-analysis reported that there was no evidence to support previous findings that patients with hypercholesterolaemia were more 10 responsive to soluble fiber than healthy individuals. Subgroup analysis of initial cholesterol concentrations showed that persons with moderate or severe hypercholesterolemia (concentrations > 6.20 mmol/L) showed only slightly larger decreases in total cholesterol that those with lower cholesterol concentrations. Nevertheless, initial LDL cholesterol was a moderately 15 significant predictor of LDL changes: 0.02 mmol/L per gram of soluble fiber, which is conservative and substantially less than in the present study. The confounding effects of dietary modification such as displacement of dietary fat and loss of body weight are largely eliminated in studies where the type and amounts of food are controlled by the investigators. Yet. in a 20 metabolic ward study. others 21 failed to find a cholesterol-lowering effect in 14 normocholesterolaemic subjects despite 9 g p-glucan from oat gum. In contrast, in a less controlled study, in which weight loss and fat displacement did occur. cholesterol lowering of 10% and 15% and LDL lowering of 15 and 21% with 2 and 8 g oat gum P-glucan in 23 25 normocholesterolaemic individuals has been reported. A possible explanation for the absent effect in the earlier study may lie in the nature of the processed p-glucan. which many have been viscous due to its low molecular mass (1000 kDa). By contrast the p-glucan consumed by the subjects in another study with had a higher molecular mass of 1200 kDa 30 that significantly lowered serum and LDL cholesterol in free living hypercholesterolemic subjects. Increased viscosity of gastrointestinal content induced by appropriate forms of p-glucan may be a key factor in cholesterol-lowering. Viscosity depends on the solubility of the p-glucan and its molecular weight, which in 35 turn is influenced by technological treatment, the cultivar and growing conditions. The hypothesis is that increased viscosity in the gut leads to an WO 01/21012 PCT/AUOO/01113 34 unstirred layer adjacent to the mucosa. This layer may serve as a physical barrier to bile acid reabsorption. which leads to increased uptake of LDL cholesterol into the liver to replenish hepatic cholesterol. 5 Table 9: Clinical Characteristics of subjects Parameter Value Age (yr) 53 + 10 Gender 8 males. 7 females Weight (kg) 76.6 + 16.81 BMI Kg/M 25.5 + 3.41 W/H ratio 1.0 + 0.68 Serum cholesterol mmol/L 6.40 + 0.80 Triglycerides mmol/L 1.22 + 0.40 Glucose mmol/L 5.32 + 0.34 WO 01/21012 PCT/AUOO/01113 35 Table 10: Plasma lipids following low, high and low p-Glucan Periods Total cholesterol (mmol/L) Low Glucan 1 6.42 + 0.7* High Glucan 6.14 + 0.53 Low Glucan 2 6.44 + 0.6** Triglycerides (mmol/L) Low Glucan 1 1.25 + 0.46 High Glucan 1.38 + 0.64 Low Glucan 2 1.43 + 0.86 LDL cholesterol mmol/L Low Glucan 1 4.59 + 0.59* High Glucan 4.17 + 0.58 Low Glucan 2 4.52 + 0.65** HDL cholesterol mmol/L Low Glucan 1 1.32 + 0.25 High Glucan 1.31 + 0.25 Low Glucan 2 1.29 + 0.25 * different from high P<0.05 ** different from high** P<0.05 5 It will be appreciated by persons skilled in the art that numerous variations and/or modifications may be made to the invention as shown in the specific embodiments without departing from the spirit or scope of the invention as broadly described. The present embodiments are. therefore, to 10 be considered in all respects as illustrative and not restrictive.

Claims (41)

1. A method of manufacturing a bran product derived from a grain, the method comprising the steps of: (a) providing a dehulled grain; 5 (b) stabilising the dehulled grain; (c) pressing or rolling the stabilised dehulled grain to a thickness in a manner that allows minimal disruption to an outer bran layer thereof; (d) milling the pressed or rolled dehulled grain to produce a first grain flour and a coarse grain bran in a manner that allows minimal disruption to 10 the outer bran layer: and (e) sieving and separating the first grain flour from the coarse grain bran; so as to provide a coarse grain bran product enriched with at least two components selected from the group consisting of p-glucan. fats and fibre each of which being derived from the grain. 15

2. The method according to claim 1 wherein steps (a) and (b) are replaced by the steps of stabilising the grain, dehulling the stabilised grain and separating the stabilised dehulled grain prior to step (c).

3. The method according to claim 1 or 2 wherein the grain is selected from the group consisting of oats. barley, wheat, rice, corn, rye, maize, and 20 millet.

4. The method according to claim 3 wherein the grain is oats.

5. The method according to any one of claims 1 to 4 wherein the p-glucan is present in an amount of greater than 10% (w/w); fats in an amount of greater than 10% (w/w); and fibre in an amount of greater than 20% (w/w). 25

6. The method according to claim 5 wherein proteinaceous material is also present in an amount of greater than 15% (w/w).

7. The method according to any one of claims 1 to 6 wherein the grain is stabilised to inactivate the majority of the enzymes in the grain, including as lipases, p-glucanases and amylases and other unwanted enzymes, which may 30 result in the destruction of nutrients in the grain.

8. The method according to claim 7 wherein the stabilising step is carried out by treatment with acids or kilning.

9. The method according to claim 8 wherein the kilning method involves heating the grain with wet steam for a period to inactivate the enzymes, 35 followed by a holding step for flavour enhancement, followed by a cooling step. WO 01/21012 PCT/AUOO/01113 37

10. The method according to claim 9 wherein a dehulled grain is wet steamed at a temperature of 75 to 150'C, preferably 95 to 100 0 C, for a period of 15 to 50 minutes, preferably, 20 to 35 minutes; the holding step is carried out at 130 to 70 0 C, preferably, 95 to 80"C for a period of 30 minutes to 3 5 hours, preferably, 1 to 2 hours; and the cooling step is out from 130 to 20'C preferably, 80 to 30'C. for a period of 15 minutes to 2 hours, preferably 30 to 60 minutes.

11. The method according to any one of claims 1 to 10 wherein a second heat treatment step is carried out after step (b) and prior to step (c) by 10 steaming, at 80 to 100C, preferably 95 to 90'C. over a period of 10 to 60 minutes. preferably 15 to 25 minutes.

12. The method according to any one of claims 1 to 11 wherein in step (c), the grain is rolled to a thickness of not less than about 900 to 1500 pLm, preferably to a thickness of about 1000 to 1100 ptm. 15

13. The method according to any one of claims 1 to 12 wherein the milling step (d) is carried out using a gristing mill or break rolls, and the sieving step (e) is carried out using a vibrosifter having a screen with pores greater than 350 4m, preferably about 350 to 600 pam. and more preferably, about 400 to 500 pm. 20

14. The method according to any one of claims 1 to 13 wherein the coarse grain bran undergoes a further sieving step to separate fine grain bran from a second pass flour.

15. The method according to claim 14 wherein the coarse bran is passed through an air classifier to remove a second pass of grain flour, the air 25 classifier, preferably has a setting of about 75 to 475 rpm, more preferably, about 150 to 350 rpm.

16. The method according to claim 15 wherein the fine grain bran has an average diameter of about 100 to 1000 pam.

17. The method according to claim 16 wherein the fine grain bran 30 undergoes a further grinding step and sieving step to separate a coarse grain bran concentrate, preferably having an average diameter of about 50 to 300 ln.

18. The method according to claim 17 wherein the coarse grain bran concentrate undergoes a further grinding step to produce a superfine grain 35 bran concentrate an average diameter of about 20 to 150 m. WO 01/21012 PCT/AUOO/01113 38

19. A method of manufacturing a bran product derived from a grain, the method comprising the steps of: (a) providing a dehulling grain; (b) stabilising the dehulled grain: 5 (c) subjecting the stabilised dehulled grain to a heat treatment step; (d) pressing or rolling the heat treated dehulled grain to a thickness in a manner that allows minimal disruption to an outer bran layer; (e) milling the pressed or rolled dehulled grain to produce a first grain flour and a coarse grain bran; 10 (f) sieving and separating the first grain flour from the coarse grain bran: (g) grinding the coarse grain bran to produce a second pass flour and a fine grain bran; (h) sieving and separating the second pass flour from the fine grain bran; (i) grinding the fine grain bran to produce a third pass of grain flour and a 15 coarse grain bran concentrate; (j) sieving and separating the third pass of grain flour from the coarse grain bran concentrate: followed by either (k) grinding the coarse grain bran concentrate to produce a superfine grain bran concentrate: or 20 (1) grinding the coarse grain bran concentrate to produce a fourth pass of grain flour and a fine grain bran concentrate; (in) sieving and separating the fourth pass of flour from the superfine grain bran concentrate: to produce a coarse fine or superfine grain bran concentrate enriched with at 25 least two components selected from p-glucan, lipids and fibre being derived from the grain.

20. The method according to claim 19 wherein P-glucan is present in an amount of greater than 10% (w/w): fats in an amount of greater than 10% (w/w): and fibre in an amount of greater than 20% (w/w). 30

21. The method according to claim 20 further including proteinaceous material present in an amount of greater than 15% (w/w).

22. The method according to claim 19 or 21 wherein the grain is selected from the group consisting of oats. barley, wheat. rice. corn, rye. maize. and millet. 35

23. The method according to claim 22 wherein the grain is oats. WO 01/21012 PCT/AU0O/01113 39

24. A bran product rich in p-glucan produced by the method according to any one of claims 1 to 18.

25. A bran product rich in P-glucan produced by the method according to any one of claims 19 to 23. 5

26. Use of a bran product according to claim 24 or 25 in the manufacture of a food and/or drink product.

27. The use according to claim 26 wherein the food product is a bakery product including bread, biscuits and pastry; a cereal product including cereal flakes, muesli bars; a powder supplement in drinks; meat and meat 10 analog products; soups, sauces, pastes; confectionary products; butters; spreads; non-frozen and frozen desserts; dairy and dairy analogs; dietary supplements; cosmetics; stockfeed; animal and veterinary care products.

28. A method of producing a bran gel or paste, the method comprising subjecting the grain bran or grain bran concentrate made by the method 15 according to any one of claims 1 18 to enzymatic starch hydrolysis.

29. The method according to claim 28 comprising treating an aqueous solution of grain bran concentrate product with an a-amylase under conditions which will hydrolyse the grain bran concentrate product to produce a bran gel. 20

30. The method according to claim 29 wherein an aqueous suspension of grain bran concentrate, a-amylase and optionally p-amylase is heated to a temperature within the operable range 50 to 95 0 C, preferably 70 to 90*C.

31. The method according to claim 29 wherein the grain bran concentrate contains 16-70%, preferably 35-45% solids; and pH is maintained between 25 5.0 to 8.0, preferably, 6.0 to 7.0.

32. The method according to claim 31 further comprising cooling the product to less than about 70 0 C, preferably less than 50'C, after about 10 to 120 minutes and adjusting the pH to 4.5 for approximately 10 to 30 minutes after which the pH is adjusted to approximately 6.5. 30

33. The method according to claim 32 wherein the final product is dehydrated to produce a bran gel powder.

34. Use of the bran gel powder produced by claim 33 in the production of drinks, ice cream, dairy and dairy analogs, frozen and non-frozen desserts, meat and meat analogs, soup, sauces, pastes, dressings, spreads, as a fat 35 replacer, stabilising syrup systems such as in food coating and binding such as syrup for food bars. WO 01/21012 PCT/AUOO/01113 40

35. A substantially shelf-stable bran product derived from grain, the bran product comprising at least two of the following components: (a) greater than 10% (w/w) p-glucan; (b) greater than 10% (w/w) lipid; and 5 (c) greater than 20% (w/w) total dietary fibre (TDF); wherein the P-glucan, lipid and TDF are derived from the grains used to make the grain bran product.

36. The bran product according to claim 35 further containing proteinaceous material in an amount of greater than 15% (w/w). 10

37. The bran product according to claim 35 or 36 wherein the P-glucan is present in an amount of 10 to 20% (w/w), lipid in an amount of 10 to 20% (w/w), and TDF in an amount of 23 to 50% (w/w).

38. The bran product according to claim 37 wherein the TDF comprises soluble fibre is in an amount of 6 to 30% (w/w) and the insoluble fibre in an 15 amount of 5 to 50% (w/w).

39. The grain bran according to any one of claims 35 to 38 further including proteinaceous material in an amount of 15 to 50% (w/w), ash in an amount of 3 to 10% (w/w), carbohydrate in an amount of 30 to 70%, and starch in an amount of 25 to 55% (w/w). 20

40. The grain bran according to claim 39 further including vitamins such as thiamine, riboflavin, niacin, alpha-tocopherol, pyridoxine and folate and minerals such as calcium, iron, potassium,. magnesium, sodium, phosphorous and zinc.

41. A substantially shelf-stable oat bran product comprising: 25 (a) 10-18% (w/w) P-glucan; (b) 12 to 15% (w/w) lipid; (c) 17 to 19% (w/w) insoluble fibre; (d) 10 to 25% (w/w) soluble fibre; (e) 18 to 26% (w/w) proteinaceous material; 30 (f) 50 to 60% (w/w) carbohydrate; and (g) 4 to 5% ash (w/w); wherein the P-glucan, fat and TDF are derived from the oats used to make the oat bran product.