AU736031B2 - Combinatorial synthesis of carbohydrate libraries - Google Patents

Description

WO 98/22487 PCTICA97/00866 -1- COMBINATORIAL SYNTHESIS OF CARBOHYDRATE LIBRARIES BACKGROUND OF THE INVENTION Field of the Invention S 5 This invention is directed to methods for synthesizing very large collections of diverse thiosaccharide derivatives optionally attached to a solid support. This invention is further directed to a library of diverse thiosaccharide derivatives.

References The followiing publications, patents and patent applications are cited in this application as superscript numbers: International Patent Application Publication No. WO 93/06121.

2 U.S. Patent No. 5,143,854, issued September 1, 1992.

Hol, W. G. et al., "Structure and Function of E. coli Heat-Labile Enterotoxin and Cholera Toxin B Pentamer", Bacterial Toxins and Virulence Factors in Disease, Ed. by J. Moss et al., Marcel Dekker, Inc. (1995).

4 Spangler, B. "Structure and Function of Cholera Toxin and Related Escherichia coli Heat-Labile Enterotoxin", Microbiological Reviews, 56(4):622-647 (1992).

S Williams Synthesis of Optically Active a-Amino Acids, Pergamon Press (1989).

6 Evans et al., J. Amer. Chem. Soc., 112:4011-4030 (1990).

WO 98/22487 WO 9822487PCTICA97/00866 -2- 7 Pu et al., J. Amer. Chem. Soc., 56:1280-1283 (1991).

Williams et al., J. Amer. Chem. Soc., 113:9276-9286 (1991).

9 Ratcliffe, et al., U.S. Patent No. 5,079,353.

J. Defaye, et al., "Thiooligosaccharides: .Their Synthesis and Reactions with Enzymes" in Studies in Natural Products Chemistry, Vol. 8, pp.

315-357, Elsevier Sciences Publishers (1991).

11 Kagen et al., Synlett, 1990, 643-650.

12 E. Hasegawa, K. Ishiyama, T. Horaguchi, T. Shimizu, J. Org.

Chem. 1991, 56, 1631-1635.

13 H. Paulsen, K. Eberstein, W. Koebernick, Tetrahedron Letters, 45-50, 4377-4380.

14 J.M. Kerr, S.C. Banville and R.N. Zuckermann, J. Am. Chem. Soc., 115:2529 (1993).

V. Nikolaiev, A. Stierandova, V. Krchnak, B. Seligmann, K.S. Lam, S.E. Salmon and M. Lebi, Pept. Res., 6:161 (1993) 16 M.C. Needels, D.G. Jones, E.M. Tate, G.L. Heinkel, L.M.

Kochersperger, W.J. Dower, R.W. Barrett and M.A. Gallop, Proc.

Nati. Acad. Sci., USA, 90: 10700 (1993) 17 M.H.J. Ohlmeyer, R.N. Swanson, L.W. Dillard, J.C. Reader, G.

Asouline, R. Kobayashi, M. Wigler and W.C. Still, Proc. Natl. Acad.

Sci. USA, 0Q:10922 (1993) is U.S. Patent No. 4,137,401, issued January 30, 1979, to R.

Lemieux et al.

19 H. H. Westal et al., "Methods of Enzymology," 34(b), 64 (1974).

20 T. Mukalyama et al., Tetrahedron Letters, 56, 5907-5908 (1968).

21 Svennerholm, A-M. et al., Current Microbiology, 1: 19-23 (1978).

WO 98/22487 PCT/CA97/00866 -3- All of the above publications, patents and patent applications are hereinf incorporated by reference in their entirety to the same extent as if each individual publication, patent or patent application was specifically and individually indicated to be incorporated by reference in its entirety.

State of the Art Compounds having biological-activity can be identified by screening diverse collections of compounds libraries of compounds) produced through either molecular biological or synthetic chemical techniques. Such screening methods include methods wherein each member of the library is tagged with a unique identifier tag to facilitate identification of compounds having biological activity' or where the library comprises a plurality of compounds synthesized at specific locations on the surface of a solid substrate wherein a receptor is appropriately labeled to identify binding to the compound, fluorescent or radioactive labels. Correlation of the labelled receptor bound to the substrate with its location on the substrate identifies the binding compound.

2 Central to these methods is the screening of a multiplicity of compounds in the library and the ability to identify the structures of the compounds which have a requisite biological activity. Preferably, in order to facilitate synthesis and identification, the compounds in the library are typically formed on solid supports wherein the compound is covalently attached to the support via a cleavable or noncleavable linking arm. In this regard, libraries of diverse compounds are prepared and then screened to identify "lead compounds" having good binding affinity to the receptor.

Pharmaceutical drug discovery relies heavily on studies of structure-activity relationships wherein the structure of "lead compounds" is typically altered to determine the effect of the alteration on activity. Alteration of the structure of the lead compounds permits evaluation of the effect of the structural alteration on activity.

Thus libraries of compounds derived from a lead compound can be created by including derivatives of the lead compound and repeating the screening procedures.

WO 98/22487 PCT/CA97/00866 -4- Ideally, the compounds are synthesized in situ on the solid support so that the support can be tagged to-identify the synthetic steps employed and/or the derivative incorporated onto the support. However, relatively simple synthetic methods to produce a diverse collection of such derivatives on the supports are often not available.

One particular class of compounds which would be useful for inclusion in screening libraries is thiosaccharide derivatives. It is well known that certain toxins and organisms bind to oligosaccharide receptors on host cells as an initial step in the pathological development of various disease conditions.' For example, heat-labile enterotoxin secreted by certain enterotoxigenic strains of Escherichia coli, and cholera toxin produced by Vibrio cholerae, are known to bind to ganglioside GMI, a glycosphingolipid situated in the outer leaflet of the host cell membrane and which has a characteristic pentasaccharide structure, i.e., Gal(3 1-3)GalNAc(3 1-4) {NeuAc(a2-3)}- Gal(o1-*4)Glc, on its surface.

3 LT has been identified as one of the causative agents of bacterial-induced traveller's diarrhea 4 and CT is known to be the causative agent of the severe diarrheal disease, cholera.

4 Additionally, many virulent organisms bacteria, virus, fungi, and the like) including enterovirulent organisms bind to cell surface receptors as part of the disease process. For example, bacteria such as Vibrio cholerae and enterotoxigenic strains of Escherichia coli can directly bind to cell surface receptors forming a colony at the point of attachment. Such binding is detrimental because it permits expressed toxin to immediately interact with the cell surface.

Accordingly, in order to develop new pharmaceutical drugs to treat various disease conditions, it would be highly desirable to be able to generate very large libraries of diverse thiosaccharide derivatives.

SUMMARY OF THE INVENTION This invention is directed to general synthetic methods for generating very large libraries of diverse thiosaccharide derivatives optionally attached to a solid support. The thiosaccharide derivative libraries provided by this invention are synthesized by reacting a thiosaccharide with a Michael acceptor, an a-(sulfonic ester) carbonyl compound or an ahalocarbonyl compound to provide for a thiosaccharide carbonyl compound. The carbonyl group of the thiosaccharide carbonyl compound can optionally be reduced to provide for a plurality of alcohol and/or amine thiosaccharide derivatives. In one embodiment, the alcohol and/or amine group of the thiosaccharide derivative is further derivatized to provide for a plurality of thiosaccharide derivatives.

In one embodiment of this invention, the thiosaccharide derivatives are covalently attached to a solid support. Solid supports containing such thiosaccharide derivatives preferably comprise a linking arm which links the solid support to the thiosaccharide derivative. The linking arm can be either cleavable or non-cleavable and when cleavable, can be used to prepare a library of either solid phase or soluble thiosaccharide derivatives. The library of thiosaccharide derivatives, whether soluble S or insoluble, can be screened to isolate individual compounds that possess some S desired biological activity. In a preferred embodiment, each compound in the library is unique.

9 Accordingly, in one of its method aspects, this invention is directed to a method for synthesizing a thiosaccharide derivative, which method comprises: providing a thiosaccharide; providing at least a stoichiometric amount of a coupling reagent selected from the group consisting of Michael acceptors, a-(sulfonic ester) carbonyl compounds and a- Z halocarbonyl compounds; and contacting the thiosaccharide and the coupling reagent under conditions which provide for a thiosaccharide carbonyl compound.

In another of its method aspects, this invention is directed to a method for synthesizing a thiosaccharide derivative on a solid support, which method comprises: R providing a thiosaccharide; providing at least a stoichiometric amount of a coupling reagent selected from Michael acceptors, a-(sulfonic ester) carbonyl compounds and a-halocarbonyl compounds wherein either the thiosaccharide or the coupling reagent is covalently attached to a solid support; and contacting the thiosaccharide and the coupling reagent under conditions which provide for a thiosaccharide carbonyl compound covalently attached to a solid support.

In preferred embodiments of this invention, each of the above methods for synthesizing a thiosaccharide derivative further comprises reducing the carbonyl group of thethiosaccharide carbonyl compound to form a group selected from hydroxy and amino derivatives. Optionally, the hydroxy or amino group can be further derivatized to form a group selected from esters, substituted amines, amides, carbamates, ureas, thiourea, thioesters and thiocarbamates.

In still another of its method aspects, this invention is directed to a method for preparing a thiosaccharide derivative library produced by synthesizing on each of a 15 plurality of solid supports a single compound wherein each compound comprises a thiosaccharide derivative, which library is synthesized in a process comprising: S. a) apportioning solid supports among a plurality of reaction vessels which supports comprise a reactive functional group covalently bound thereto which group is capable of covalently binding a thiosaccharide at a position other than the thiol group; b) contacting the supports in each reaction vessel with a unique thiosaccharide under conditions wherein the thiosaccharide is covalently attached to the solid S. supports through the reactive functional group; c) pooling the supports; d) apportioning the supports from above among a plurality of reaction vessels; and e) contacting the supports in each reaction vessel from above with a unique coupling reagent selected from the group consisting of Michael acceptors, a- (sulfonic ester) carbonyl compounds and a-halocarbonyl compounds under conditions SRA4 ich provide for a thiosaccharide carbonyl compound covalently bound to said support.

And, in yet another of its method aspects, this invention is directed to a od for preparing a thiosaccharide derivative library produced by synthesizing on each of a plurality of solid supports a single compound wherein each compound comprises a thiosaccharide derivative, which library is synthesized in a process comprising: a) apportioning solid supports among a plurality of reaction vessels which supports comprise.a reactive functional group covalently bound thereto which group is capable of covalently binding a coupling reagent; b) contacting the supports in each reaction vessel with a unique coupling reagent selected from the group consisting of Michael acceptors, a-(sulfonic ester) carbonyl compounds and a-halocarbonyl compounds under conditions wherein the coupling reagent is covalently attached to the solid supports through the reactive functional group; c) pooling the supports; d) apportioning the supports from above among a plurality of reaction vessels; and e) contacting the supports in each reaction vessel from above with a 15 unique thiosaccharide under conditions which provide for a thiosaccharide carbonyl compound covalently bound to said support.

In preferred embodiments of this invention, each of the above methods for preparing a thiosaccharide derivative library exemplified in procedures through (e) further comprises: pooling the supports from procedure 20 apportioning the supports from above among a plurality of reaction vessels; and reducing the carbonyl group of the thiosaccharide carbonyl compound to form a group selected from hydroxy and amino derivatives. Still further, such methods :optionally include the further steps of: pooling the supports from procedure (h) above; apportioning the supports from above among a plurality of reaction 25 vessels; and derivatizing the hydroxyl or amine groups to form a functional group selected from esters, substituted amines, amides, carbamates, ureas, thioureas, thioesters and thiocarbamates.

The methods described above can be used to create a library of diverse 1T ZI L thiosaccharide derivatives. Accordingly, in one of its composition aspects, this invention is directed to a library of diverse thiosaccharide derivatives comprising a plurality of OT COs solid supports having a plurality of covalently bound thiosaccharides derivatives, WO 98/22487 PCT/CA97/00866 -8wherein the thiosaccharide derivative bound to each of said supports is substantially homogeneous and further wherein the thiosaccharide derivative bound on one support is different from the thiosaccharide derivatives bound on the other supports and further wherein said thiosaccharide derivative is represented by the formula

R

3 Saccharide R4

R

1 R2 wherein R' is selected from the group consisting of hydrogen, alkyl, substituted alkyl, alkenyl, alkaryl, alkoxyalkyl, aryl, cycloalkyl, cycloalkenyl, heteroaryl, heterocyclic, thioalkoxyalkyl and a linking arm covalently linking the compound of formula I' to the support;

R

2 is selected from the group consisting of hydrogen, alkyl, substituted alkyl, alkenyl, alkaryl, alkoxyalkyl, aryl, cycloalkyl, cycloalkenyl, heteroaryl, heterocyclic, thioalkoxyalkyl and a linking arm covalently linking the compound of formula I' to the support;

R

3 is selected from the group consisting of hydrogen, alkyl, substituted alkyl, alkenyl, alkaryl, alkoxyalkyl, aryl, cycloalkyl, cycloalkenyl, heteroaryl,-heterocyclic, thioalkoxyalkyl and a linking arm covalently linking the compound of formula I' to the support; or R' and R 2 or R' and R 3 or R 2 and R 3 or R

I

R

2 and R 3 can be joined, together with the carbon atoms to which R' and/or R 2 and/or R 3 are attached, to form a cycloalkyl, cycloalkenyl or heterocyclic ring;

R

4 is selected from the group consisting of -XR 5

-XC(W)R

6 and -XC(W)X'R 7 wherein W is selected from the group consisting of oxygen, sulfur and NH; and X and X' are each independently selected from the group consisting of oxygen, sulfur and -NR 9 wherein R 9 is selected from the group consisting of hydrogen and alkyl; or when R 4 is -XR 5 and R 5 is not hydrogen, X can also be selected from the group consisting of and

-SO

2

R

5 is selected from the group consisting of hydrogen, alkyl, alkenyl, alkaryl, alkoxyalkyl, aryl, cycloalkyl, cycloalkenyl, heteroaryl, heterocyclic, thioalkoxyalkyl and a linking arm covalently linking the compound of formula I' to the support, and when X is -NR 9 then R 5 together with X can form an amino acid selected from the group consisting of glycine, P-alanine, leucine, histidine, tryptophan and arginine; or R 5 and R', or R 5 and R 2 or R 5 and R 3 can be joined, together with X of the -XR 5 group and the carbon atoms to which R' and/or R 2 and/or R 3 are attached, to form a heterocyclic ring; R' is selected from the group consisting of alkyl, alkenyl, alkaryl, alkoxyalkyl, 15 aryl, cycloalkyl, cycloalkenyl, heteroaryl, heterocyclic, thioalkoxyalkyl and a linking arm covalently linking the compound of formula I' to the support; or R 6 and R 1 or R' and R 2 or R 6 and R 3 can be joined, together with the moiety of the

XC(W)R

6 group and the carbon atoms to which R' and/or R 2 and/or R 3 are attached, to form a heterocyclic ring; 20 R 7 is selected from the group consisting of alkyl, alkenyl, alkaryl, alkoxyalkyl, aryl, cycloalkyl, cycloalkenyl, heteroaryl, heterocyclic, thioalkoxyalkyl and a linking arm covalently linking the compound of formula I' to the support; or R 7 and or R 7 and R 2 or R 7 and R 3 can be joined, together with the moiety of the

XC(W)X'R

7 group and the carbon atoms to which R' and/or R 2 and/or R 3 are attached, to form a heterocyclic ring; R is selected from the group consisting of alkyl, alkenyl, alkaryl, alkoxyalkyl, aryl, cycloalkyl, cycloalkenyl, heteroaryl, heterocyclic, thioalkoxyalkyl and a linking arm covalently linking the compound of formula I' to the support; or R 8 and or R' d R 2 or RI and R 3 can be joined, together with the moiety of the -o0 S 30 (W)XR group and the carbon atoms to which R 2 and/or R 3 are attached, to form a heterocyclic ring; WO 98/22487 PCT/CA97/00866 Y is selected from the group consisting of sulfur, and -S(0) 2 n is an integer equal to 0 or 1; and pharmaceutically acceptable salts thereof; wherein the saccharide is selected from the group consisting of a monosaccharide, an oligosaccharide, monosaccharide-Z- and oligosaccharide-Z-, wherein Z is a linking arm covalently linking the compound of formula I to the solid support; with the proviso that only one of R 2

R

3

R

4

R

6

R

7 R' and Z is linked to the solid support.

BRIEF DESCRIPTION OF THE DRAWINGS Figure 1 illustrates a preferred reaction scheme for synthesizing a library of diverse thiosaccharide derivatives using an ca,-unsaturated carbonyl compound, i.e., cyclohept-2-en- 1-one.

Figure 2 illustrates a preferred reaction scheme for synthesizing a library of diverse thiosaccharide derivatives using an a-halocarbonyl compound, i.e, 2chlorocyclohexanone.

DETAILED DESCRIPTION OF THE INVENTION This invention is directed to libraries of diverse thiosaccharide derivatives optionally attached to a solid support and to methods for generating such libraries.

However, prior to describing this invention-in further detail, the following terms will first be defined.

Definitions "Acyl" refers to the groups alkyl-C(O)-, aryl-C(O)-, and heteroaryl-C(O)where alkyl, aryl and heteroaryl are as defined herein.

"Acylamino" refers to the group -C(O)NRR where each R is independently hydrogen or alkyl.

"Acyloxy" refers to the groups alkyl-C(O)O-, aryl-C(O)O-, heteroaryl-C(O)O-, and heterocyclic-C(O)O- where alkyl, aryl, heteroaryl and heterocyclic are as defined herein.

WO 98/22487 PCT/CA97/00866 -11- "Alkaryl" refers to -alkylene-aryl groups preferably having from 1 to 8 carbon atoms in the alkylene-moiety-and from 6 to 10 carbon atoms in the aryl moiety. Such alkaryl groups are exemplified by benzyl, phenethyl and the like.

"Alkoxy" refers to the group alkyl-O-. Such alkoxy groups include, by way of example, methoxy, ethoxy, n-propoxy, iso-propoxy, n-butoxy, tert-butoxy, secbutoxy, n-pentoxy, n-hexoxy, 1,2-dimethylbutoxy, and the like.

"Alkoxyalkyl" refers to the group -alkylene-O-alkyl which includes by way of example, methoxymethyl (CH 3

OCH

2 methoxyethyl (CH 3

-O-CH

2

CH

2 and the like.

"Alkenyl" refers to alkenyl groups preferably having from 2 to 8 carbon atoms and more preferably 2 to 6 carbon atoms and having at least 1 and preferably from 1- 2 sites of alkenyl unsaturation. Such alkenyl groups include ethenyl (-CH=CH 2 npropenyl allyl) (-CH 2 CH= CH), iso-propenyl (-C(CH 3

CH

2 and the like.

"Alkyl" refers to monovalent alkyl groups preferably having from 1 to 8 carbon atoms and more preferably 1 to 6 carbon atoms. This term is exemplified by groups such as methyl, ethyl, n-propyl, iso-propyl, n-butyl, iso-butyl, n-hexyl, and the like.

"Substituted alkyl" refers to a branched or straight chain alkyl group of from 1 to 8 carbon atoms having from 1 to 3 substituents selected from the group consisting of hydroxy, acyl, acylamino, acyloxy, alkoxy, alkenyl, alkynyl, amino, aminoacyl, aryl, aryloxy, carboxy, carboxyalkyl, cyano, cycloalkyl, guanidino, halo, heteroaryl, heterocyclic, nitro, thiol, thioaryloxy, thioheteroaryloxy, and the like. Preferred substituents include hydroxy and amino.

"Alkylene" or "alkyldiyl" refers to divalent alkylene groups preferably having from 1 to 8 carbon atoms and more preferably 1 to 6 carbon atoms. This term is exemplified by groups such as methylene (-CH 2 ethylene (-CH 2

CH

2 the propylene isomers -CH 2

CH

2

CH

2 and -CH(CH 3

)CH

2 and the like.

"Alkynyl" refers to alkynyl groups preferably having from 2 to 8 carbon atoms and more preferably 2 to 6 carbon atoms and having at least 1 and preferably from 1- 2 sites of alkynyl unsaturation. Such alkynyl groups include ethynyl CH), propargyl (-CH 2 C CH) and the like.

WO 98/22487 PCT/CA97/00866 -12- "Amino acid" refers to any of the naturally occurring amino acids, as well as synthetic analogs and derivatives thereof. a-Amino acids comprise a carbon atom to which is bonded an amino group, a carboxy group, a hydrogen atom, and a distinctive group referred to as a "side chain". The side chains of naturally occurring amino acids are well known in the art and include, for example, hydrogen as in glycine), alkyl as in alanine, valine, leucine, isoleucine, proline), substituted alkyl as in threonine, serine, methionine, cysteine, aspartic acid, asparagine, glutamic acid, glutamine, arginine, and lysine), alkaryl as in phenylalanine and tryptophan), substituted arylalkyl as in tyrosine), and heteroarylalkyl as in histidine). One of skill in the art will appreciate that the term "amino acid" can also include and w-amino acids, and the like. Unnaturalamin acids are also known in the art, as set forth in, for example, Williams 3 Evans et al.

4 Pu et al.

5 Williams et al.

6 and all references cited therein. Stereoisomers D-amino acids) of the twenty conventional amino acids, unnatural amino acids such as a,adisubstituted amino acids and other unconventional amino acids may also be suitable components for compounds of the present invention. Examples of unconventional amino acids include: 4-hydroxyproline, 3-methylhistidine, 5-hydroxylysine, and other similar amino acids and imino acids 4-hydroxyproline).

"Aminoacyl" refers to the group -NRC(O)R where each R is independently hydrogen or alkyl.

The term "amino derivative(s)" refers to a primary, secondary or tertiary amine compound produced by reductive amination of a thiosaccharide carbonyl compound in the presence of ammonia or an amine, including amino acids and derivatives thereof.

"Aryl" refers to an unsaturated aromatic carbocyclic group of from 6 to 14 carbon atoms having a single ring phenyl) or multiple condensed rings naphthyl or anthryl). Preferred aryls include phenyl, naphthyl and the like.

Unless otherwise constrained by the definition for the aryl substituent, such aryl groups can optionally be substituted with from 1 to 3 substituents selected from the group consisting of hydroxy, acyl, acyloxy, alkyl, substituted alkyl, alkoxy, alkenyl, alkynyl, aminoaminoacyl, aryl, aryloxy, carboxy, carboxyalkyl, cyano, halo, nitro, heteroaryl, trihalornethyl and the like. Preferred substituents include alkyl, alkoxy, halo, carboxy, cyano, nitro, trihalomethyl, and thioalkoxy.

"Aryloxy" refers to the group aryl-O.- where the aryl group is as defined herein including optionally substituted aryl groups as also defined herein.

"Carboxy" .refers to the group -COOH.

"Carboxyalkyl" refers to the group -C(O)O-alkyl where alkcyl is as defined herein.

The term "coupling reagent" refers to Michael acceptors and ot-halocarbonyl compounds. "Michael acceptors" refers to a,fl-unsaturated carbonyl compounds having the general formula (II): 0

HH

wherein R' and R 3 are as defined herein; or R'CH-CR 2 C(0)XRI, wherein R', RI, R" and X are as defined herein. Such Michael acceptors include, by way of example, cxkunsaturated aldehydes, ci,ft-unsaturated ketones, c,O-unsatura ted esters, a,06-unsaturated thioesters, ot,f-unsaturated amides, and the like. "at-Halocarbonyl compounds" refers to compounds having the general formula: Q-CHR'-C(0)R 2 wherein R'and R' rea defined herein, and Q is chloro, bromo or iodo. Suh e halocarbonyl compounds include, by way of example, at-chloroaldehydes, t-' bromoaldehydes, a-iodoaldehydes, a-chloroketones, cx-bromoketones, c-iodoketones and the likce.

"Cycloalkyl" refers to cyclic alkyl groups or cyclic alkyl rings of from 3 to 8 carbon atoms having a single cyclic ring or multiple condensed rings which can be optionally substituted with from 1 to 3 substituents selected from the group consisting of hydroxy, acyl, acyloxy, alkyl, substituted alkyl, alkylene, alkoxy, alkenyl, alkcynyl, ~~1~mino, aminoacyl, aryl, aryloxy, carboxy, carboxyallcyl, cyano, halo, nitro, ~,~heteroaryl, trihalomethyl and the like. Preferred substituents include ailkyl, alkoxy, halo, carboxy, cyano, nitro, trihalomethyl, and thioalkoxy. Such cycloalcyl groups WO 98/22487 PCT/CA97/00866 -14include, by way of example, single ring structures such as cyclopropyl, cyclobutyl, cyclopentyl, cyclooctyl, 1-methylcyclopropyl, 2-methylcyclopentyl, 2methylcyclooctyl, and the like, or multiple ring structures such as adamantanyl and the like, and spiro compounds. Examples of suitable cycloalkyl rings include single ring structures such as cyclopentane, cyclohexane, cycloheptane, cyclooctane, and the like, or multiple ring structures such as bicyclo[2.2.1]heptane, bicyclo[3.2.1]octane, and the like. Preferred cycloalkyl rings include cyclopentane, cyclohexane, cycloheptane and bicyclo[3.2.1]octane.

"Cycloalkenyl" refers to cyclic alkenyl groups or cyclic alkenyl rings of from 4 to 8 carbon atoms having a single cyclic ring and at least one point of-internal unsaturation which can be optionally substituted with from 1 to 3 substituents selected from the group consisting of hydroxy, acyl, acyloxy, alkyl, substituted alkyl, alkylene, alkoxy, alkenyl, alkynyl, amino, aminoacyl, aryl, aryloxy, carboxy, carboxyalkyl, cyano, halo, nitro, heteroaryl, trihalomethyl and the like. Preferred substituents include alkyl, alkoxy, halo, carboxy, cyano, nitro, trihalomethyl, and thioalkoxy. Examples of suitable cycloalkenyl groups include, for instance, cyclobut- 2-enyl, cyclopent-3-enyl, cyclooct-3-enyl and the like. Such cycloalkenyl rings include, by way of example, cyclopentene, cyclohexene, and the like.

"Halo" or "halogen" refers to fluoro, chloro, bromo and iodo and preferably is either chloro or bromo.

"a-Halocarbonyl compound" refers to a compound having the general formula:

Q-CHR'-C(O)R

2 wherein R' and R 2 are as defined herein, and Q is chloro, bromo or iodo. Such a-halocarbonyl compounds include, by way of example, achloroaldehydes, a-bromoaldehydes, a-iodoaldehydes, a-chloroketones, abromoketones, a-iodoketones and the like.

"Heteroaryl" refers to a monovalent aromatic carbocyclic group of from 2 to 8 carbon atoms and 1 to 4 heteroatoms selected from oxygen, nitrogen and sulfur within the ring.

Unless otherwise constrained by the definition for the heteroaryl substituent, such heteroaryl groups can be optionally substituted with 1 to 3 substituents selected from the group consisting of alkyl, substituted alkyl, alkoxy, aryl, aryloxy, halo, WO 98/22487 PCT/CA97/00866 nitro, heteroaryl, thioalkoxy, thioaryloxy and the like. Such heteroaryl groups can have a single ring pyridyl or furyl) or multiple condensed rings indolizinyl or benzothienyl). Preferred heteroaryls include pyridyl, pyrrolyl and furyl.

"Heterocycle" or "heterocyclic" refers to a monovalent saturated or unsaturated group having a single ring or multiple condensed rings, from 1 to 8 carbon atoms and from 1 to 4 hetero atoms selected from nitrogen, sulfur or oxygen within the ring. For the purposes of this application, the term "heterocycle" or "heterocyclic" does not include carbohydrate rings mono- or oligosaccharides).

Unless otherwise constrained by the definition for the heterocyclic substituent, such heterocyclic groups can be optionally substituted with 1 to 3 substituents selected from the group consisting of alkyl, substituted alkyl, alkylene, alkoxy, aryl, aryloxy, halo, nitro, heteroaryl, thioalkoxy, thioaryloxy and the like. Such heteroaryl groups can have a single ring pyrrolidinyl, piperidinyl, morpholinyl or tetrahydrofuranyl) or multiple condensed rings indolinyl).

S Examples of nitrogen heterocycles and heteroaryls include, but are not limited to, pyrrole, imidazole, pyrazole, pyridine, pyrazine, pyrimidine, pyridazine, indolizine, isoindole, indole, indazole, purine, quinolizine, isoquinoline, quinoline, phthalazine, naphthylpyridine, quinoxaline, quinazoline, cinnoline, pteridine, carbazole, carboline, phenanthridine, acridine, phenanthroline, isothiazole, phenazine, isoxazole, phenoxazine, phenothiazine, imidazolidine, imidazoline, piperidine, piperazine, indoline and the like.

"Michael acceptor" refers to an ca,-unsaturated carbonyl compound having the general formula (II): 0 R'-CH= C-C-R 2

II

R

3 wherein R 2 and R 3 are as defined herein; or R'CH=CR 2

-C(O)XR

8 wherein R',

R

2

R

8 and X are as defined herein. Such Michael acceptors include, by way of WO 98/22487 PCT/CA97/00866 -16example, ca,3-unsaturated aldehydes, a,3-unsaturated ketones, a,3-unsaturated esters, a,c-unsaturated thioesters, ca,3-unsaturated amides and the like.

"Thioalkoxyalkyl" refers to the group -alkylene-S-alkyl which includes by way of example, thiomethoxymethyl (CH 3

SCH

2 thiomethoxyethyl (CH 3

-S-CH

2

CH

2 and the like.

"Thiol" refers to the group -SH.

"Thioalkoxy" refers to the group -S-alkyl wherein the alkyl group is as defined herein.

"Thioaryloxy" refers to the group aryl-S- wherein the aryl group is as defined herein, including optionally substituted aryl groups as also defined herein; "Thioheteroaryloxy" refers to the group heteroaryl-S- wherein the heteroaryl group is as defined herein, including optionally substituted heteroaryl groups as also defined herein.

The term "thiosaccharide" refers to a monosaccharide or oligosaccharide having 2 to about 8 saccharide units wherein at least one, and preferably 1 or 2, of the hydroxyl groups of the saccharide is replaced with a thiol group. Preferably, the thiosaccharide is an animal saccharide. The term "animal saccharide" refers to a saccharide which is naturally expressed by one or more animals, such as mammals, birds or fish. Preferably, the animal saccharide is a mammalian saccharide. In particular, preferred mammalian saccharides include D-galactose, D-glucose, Dmannose, D-xylose, D-glucuronic acid, N-acetyl-D-glucosamine, N-acetyl-Dgalactosamine, sialyic acid, iduronic acid, L-fucose, and the like. Included within the definition of this term are acylated, phosphorylated and sulfated derivatives of animal saccharides.

The term "thiosaccharide carbonyl compound" refers to a compound having the formula (III):

R

3 Saccharide- Y ,P III WO 98/22487 PCT/CA97/00866 -17wherein R 2

R

3 n and saccharide are as defined herein.

The term "substrate" or "solid support" refers to a material having a rigid or semi-rigid surface which contains or can be derivatized to contain reactive functionality which covalently links a compound to the surface thereof. Such materials are well known in the art and include, by way of example, silicon dioxide supports containing reactive Si-OH groups, polyacrylamide supports, polystyrene supports, polyethyleneglycol supports, and the like. Such supports will preferably take the form of small beads, pellets, disks, or other conventional forms, although other forms may be used. In some embodiments, at least one surface of the substrate will be substantially flat.

In one embodiment, the activated ketone compound is covalently attached directly to the solid support or is attached to the support via a linking arm. Linking arms are well known in the art and include, by way of example only, conventional linking arms such as those comprising ester, amide, carbamate, ether, thio ether, urea, amine groups and the like. The linking arm can also be a covalent bond. The linking arm can be cleavable or non-cleavable.

"Cleavable linking arms" refer to linking arms wherein at least one of the covalent bonds of the linking arm which attaches the compound to the solid support can be readily broken by specific chemical reactions thereby providing for compounds comprising activated ketone groups free of the solid support ("soluble compounds").

The chemical reactions employed to break the covalent bond of the linking arm are selected so as to be specific for bond breakage thereby preventing unintended reactions occurring elsewhere on the compound. The cleavable linking arm is selected relative to the synthesis of the compounds to be formed on the solid support so as to prevent premature cleavage of this compound from the solid support as well as not to interfere with any of the procedures employed during compound synthesis on the support. Suitable cleavable linking arms are well known in the art.

A particularly preferred linking arm is illustrated in the formula: (saccharide)-NH-(CH 2 ),-NHC(O)NH-(support) WO 98/22487 PCT/CA97/00866 -18wherein m is an integer of from 2 to about 10. Preferably, m is 6.

"Non-cleavable linking arms" refer to linking arms wherein the covalent bond(s) linking the activated ketone compound to the solid support can only be cleaved under conditions which chemically alters unintended parts of the structure of the compound attached thereto.

The term "substantially homogeneous" refers to collections of molecules wherein at least 80%, preferably at least about 90% and more preferably at least about 95% of the molecules are a single compound or stereoisomers thereof.

The term "stereoisomer" refers to a chemical compound having the same molecular weight, chemical composition, and constitution as another, but with the atoms grouped differently. That is, certain identical chemical moieties are at different orientations in space and, therefore, when pure, have the ability to rotate the plane of polarized light. However, some pure stereoisomers may have an optical rotation that is so slight that it is undetectable with present instrumentation. The compounds described herein may have one or more asymmetrical carbon atoms and therefore include various stereoisomers. All stereoisomers are included within the scope of the invention.

When chiral centers are found in the thiosaccharide derivatives of this invention, it is to be understood that this invention encompasses all possible stereoisomers. For example, when n is 0 in formula I, the carbon atoms to which R 1 and R 2 are attached may have an R,R or R,S or S,R or S,S configuration. Similarly, when n is 1, the carbon atoms to which R 2 and R 3 are attached may have an R,R,R or S,R,R or R,S,R or R,R,S or S,S,R or S,R,S or R,S,S or S,S,S configuration.

The term "removable protecting group" or "protecting group" refers to any group which when bound to a functionality such as hydroxyl, amino, or carboxyl groups prevents reactions from occurring at these functional groups and which protecting group can be removed by conventional chemical or enzymatic steps to reestablish the functional group. The particular removable protecting group employed is not critical.

WO 98/22487 PCT/CA97/00866 -19- The term "toxin" refers to a compound produced by an organism which causes or initiates the development of a noxious, poisonous or deleterious effect in a host presented with the toxin. Such deleterious conditions may include fever, nausea, diarrhea, weight loss, neurologic disorders, renal disorders, hemorrhage, and the like.

As used herein, the term "toxin" includes bacterial toxins, such as cholera toxin, heatliable and heat-stable toxins of E. coli, toxins A and B of Clostridiwn difficile, aerolysins, hemolysins, and the like; toxins produced by protozoa, such as Giardia; toxins produced by fungi; and the like. Included within this term are exotoxins, i.e., toxins secreted by an organism as an extracellular product, and enterotoxins, i.e., toxins present in the gut of an organism.

The terms "heat-labile enterotoxin" or "LT" refer to an enterotoxin of enterotoxigenic E. coli which initiates traveller's diarrhea and related conditions. This toxin has a lectin-like activity.

The term "traveller's diarrhea" refers to diarrhea of sudden onset, often accompanied by abdominal cramps, vomiting and fever that occurs sporadically in traveller's, usually during the first week of a trip. This diarrhea is most commonly caused by enterotoxigenic E. coli.

The term "cholera" refers to an acute epidemic infectious disease caused by Vibrio cholerae, wherein a soluble toxin elaborated in the intestinal tract by the Vibrio alters the permeability of the mucosa, causing a profuse watery diarrhea, extreme loss of fluid and electrolytes, and a state of dehydration and collapse, but no gross morphologic change in the intestinal mucosa.

The terms "cholera toxin" or "CT" refer to an enterotoxin of V. cholerae which initiates cholera and related conditions. This toxin has a lectin-like activity.

The phrase "inhibit(s) the binding of a toxin to its receptor" means that a compound inhibits the binding of a toxin to its receptor by at least 20%. For example, useful binding inhibition assays may measure inhibition-of binding to ganglioside GDib or ganglioside GM,, neutralization of cytotoxic activity, or the like.

Such binding is reported herein as percent toxin activity remaining so that those compounds which result in about 80% or less toxin activity remaining under the WO 98/22487 PCT/CA97/00866 bioassay conditions disclosed herein are deemed to inhibit the binding of a toxin to its receptor.

The phrase "inhibit(s) the binding of heat-labile enterotoxin (LT) and/or cholera toxin (CT) to an LT and/or CT receptor" means that a compound inhibits the binding of LT and/or CT to an LT and/or CT receptor by at least 20 The phrase "inhibit(s) the binding of an organism to its cell surface receptor" means that a compound inhibits the binding of an organism, such as a bacterium, a virus, a protozoan, a fungus, and the like, to its cell surface receptor. For example, for organisms such as Vibro cholera or enterotoxigenic strains of E. coli, a compound is said to inhibit binding of an organism to a cell surface receptor if it reduces binding of a bacterial surface adhesion antigen, such as CFA I pili, by at least The term "pharmaceutically acceptable salt" refers to pharmaceutically acceptable salts of a compound of formula I which salts are derived from a variety of organic and inorganic counter ions well known.in the art and include, by way of example only, sodium, potassium, calcium, magnesium, ammonium, tetraalkylammonium, and the like; and when the molecule contains a basic functionality, salts of organic or inorganic acids, such as hydrochloride, hydrobromide, tartrate, mesylate, acetate, maleate, oxalate and the like.

For purpose of this application, all sugars are referenced using conventional three letter nomenclature. All sugars are assumed to be in the D-form unless otherwise noted, except for fucose, which is in the L-form. Further, all sugars are in the pyranose form.

General Synthetic Procedures 1. Method for Synthesizing Thiosaccharide Derivatives In one aspect, the methods of this invention involve the novel addition of a thiosaccharide to a coupling reagent selected from the group consisting of Michael reagents and a-halocarbonyl compounds.

Specifically, the thiosaccharide derivatives of this invention are typically prepared by reaction of a suitably protected thiosaccharide intermediate with an a,13unsaturated carbonyl compound or an a-halocarbonyl compound to provide for a -21thiosaccharide carbonyl compound. The carbonyl group of the thiosaccharide carbonyl compound is then optionally reduced to provide for a plurality of alcohol and/or amine thiosaccharide derivatives. In one embodiment, the alcohol and/or amine group of the thiosaccharide derivative is further derivatized to provide for a plurality of thiosaccharide derivatives.

The a,0-unsaturated carbonyl compounds employed in preparing the thiosaccharide derivatives of this invention preferably have the general formula (II): 0 R'-CH=C-C-R

II

R

3 wherein R 2 and R 3 are as defined above; or R'CH=CR-C(O)XR', wherein R', R, R' and X are as defined above. These compounds are either commercially available or can be prepared from commercially available materials using art recognized procedures. For example, such compounds can be prepared via a Wittig reaction from an aldehyde, R'CHO, and a 0-carbonyl phosphorane, such as (Ph) 3

PC(R)C(O)R

2 Preferred ca,3-unsaturated carbonyl compounds for use in this invention include, by way of example, cyclopent-2-en-l-one, 4,4-dimethylcyclopent-2-en-1-one, cyclohex-2-en-l-one, 4,4-dimethylcyclohex-2-en-l-one, 6,6-dimethylcyclohex-2-en-1- S one, cyclohept-en-1-one, and 3-methylene-2-norboranone.

The c-halocarbonyl compounds employed in preparing the thiosacchatide derivatives of this invention preferably have the general formula: Q-CHR'-C(O)R 2 wherein R' and R 2 are as defined above, and Q is chloro, bromo or iodo. Such compounds are either commercially available or can be prepared from commercially available materials using art recognized procedures. Preferred a-halocarbonyl compounds for use in this invention include, by way of example, 2chlorocyclopentanone and 2-chlorocyclohexanone. Alternatively, carbonyl compounds having a leaving group other than a halogen in the a-position may be employed.

P:\OPER\b. ,54-98 WAMdo-24M)AI I -21A- Suitable leaving groups include, by way of illustration, various sulfonic ester groups, such as a-(sulfonic ester)carbonyl compounds having the general formula: Q'-CHR'

C(O)R

2 where Q' is a sulfonic ester and R 1 and R(2 are as defined above. Preferred sulfonic ester groups include tosylate, mesylate, brosylate and nosylate groups and the like, and fluorinated sulfonic ester groups, such as triflate, nonaflate and tresylate groups and the like.

The sugars employed in this invention are any thiol containing saccharides or oligosaccharides wherein the thiol substitution is at any position of the thiosaccharide.

For example, thiolactose having a thiol group at the 1, 2, 3, 6, 4' or 6' can be used. Methods for chemically modifying saccharides to introduce suitable substitution are well known in the art as illustrated in Ratcliffe, et al.

9 and references cited therein as well as by Defaye 0 For example, 1-thiosaccharides can be prepared by reacting the saccharide with an acylating agent to convert all of the hydroxyl groups to acyl groups. The 1-acyl group is then selectively converted to the 1thioacetyl group by reaction with an excess of thiolacetic acid. Hydrolysis then provides for the 1-thiosaccharide.

Alternatively, selective protection of the hydroxyl groups of the saccharide provides for one or more free hydroxyl groups which can be converted into appropriate leaving groups, such as mesyl or halo groups, by conventional chemistry well known in the art. Such leaving groups can then be displaced to afford the :corresponding thiol groups. See, for example, International Patent Application Serial S:.i No. PCT/CA92/00242. Specifically, a mesyl group is selectively introduced at one of the hydroxyl groups and then reacted with a thioacetyl group (for example potassium thioacetate) to provide for the corresponding thioacetate derivative. Treatment of this compound with a mild base provides for the corresponding thio group.

::.The resulting thiosaccharide is then reacted with a coupling reagent selected from the group consisting of Michael acceptors and a-halocarbonyl compounds.

Typically, this reaction is conducted by contacting the thiosaccharide with at least one equivalent, preferably 1 to 1.2 equivalents, of the coupling reagent in an inert diluent, such as dichloromethane, at a temperature of from about -40"C to about 50 0 C for about 1 to about 6 hours to afford a thiosaccharide carbonyl compound. In a preferred embodiment, when the thiosaccharide reagent is attached to a solid support, Se coupling reagent is preferable used in excess to maximize the yield of the resulting tiosaccharide carbonyl compound. Alternatively, when the the coupling reagent is WO 98/22487 PCT/CA97/00866 -23attached to a solid support, the thiosaccharide is preferably used in excess relative to the coupling reagent.

The carbonyl group of the thiosaccharide carbonyl compound can then be optionally reduced using a reducing agent to provide for an alcohol derivative.

Preferably, this reduction is conducted by contacting the thiosaccharide carbonyl compound with sodium borohydride, preferably about 1.2 to about 2.0 equivalents of sodium borohydride based on the carbonyl compound. Generally, this reaction is conducted in an inert diluent, such as tetrahydrofuran, isopropanol and mixture thereof, at a temperature of about 25 0 C to about 30*C for about 0.5 to about hours, to afford the alcohol derivative.

Alternatively, the carbonyl group of the thiosaccharide carbonyl compound can be reductively aminated to-provide for an amine derivative. In this reaction,.the thiosaccharide carbonyl compound is contacted with an excess of ammonium acetate and at least one equivalent of sodium cyanoborohydride based on the carbonyl compound. This reaction is typically conducted in an inert diluent, such as methanol, tetrahydrofuran and mixtures thereof, at a temperature of about 25 0 C to about for about 1 to about 72 hours.

The thiosaccharide carbonyl compound can also be reductively aminated in the presence of a primary or secondary amine to provide for amine derivatives.

Preferably the amine used in the reductive amination is an amino acid or a derivative thereof, such as amino acid esters. Typically, this reaction is conducted by contacting the thiosaccharide carbonyl compound with a molar excess of an amino acid ester, such as the methyl ester or the tert-butyl ester, preferably with 10 equivalents based on the carbonyl compound, in the presence of at least one molar equivalent, preferably about 1.0 to about 1.2 equivalents, of sodium cyanoborohydride.

Typically, this reaction is conducted in an essentially anhydrous inert diluent, such as acetonitrile, at a temperature of about 25"C to about 30 C for about 1 to about 72 hours. Subsequently, the ester group of the amino acid can be cleaved using standard conditions to provide the corresponding carboxylic acid.

In a preferred embodiment, the alcohol and/or amine derivatives prepared as described above are further derivatized to forii a group selected from esters, WO 98/22487 PCT/CA97/00866 -24substituted amines, amides, carbamates, ureas, thioureas, thioesters and thiocarbamates. Methods for derivatizing alcohols and/or amines to provide for such functional groups are well known to those skilled in the art. For example, alcohols and amines can be reacted with acyl halides to form esters and amides, respectively.

Amines can also be reductively alkylated to form substituted amines. Similarly, alcohols and amines can be reacted with isocyantes, among other reagents, to afford carbamates and ureas, respectively. Conditions for such reactions are well recognized in the art.

Preferred embodiments of this invention are illustrated in Figures 1 and 2.

Figure 1 illustrates the synthesis of various 1-thiogalactose derivatives from cyclohept- 2-en-l-one. Figure 2 illustrates the synthesis of various 1-thiogalactose from 2chlorocyclohexanone. It will be readily apparent to those of ordinary skill in the art that the synthetic procedure illustrated in Figures 1 and 2 and following reaction conditions can be modified by selecting the appropriate starting materials and reagents to allow the preparation of a plurality of 1-thiogalactose derivatives.

As shown in Figure 1, D-galactose is perlauroylated by contacting D-galactose with at least 5 equivalents, and preferably 10 equivalents, of lauroyl chloride. This reaction is generally conducted in an inert diluent, such pentane, hexane, dichloromethane and the like, using a tertiary amine such as pyridine or triethylamine to neutralize the hydrochloric acid generated during the reaction. Preferably, a catalytic amount of 4-(N,N-dimethylamino)pyridine is added to the reaction mixture to facilitate this reaction. Typically, this reaction is conducted at a temperature of from about -78*C to about 30°C for about 0.5 to about 96 hours to afford 1,2,3,4,6-penta- O-lauroyl-a-D-galactopyranose, 1, in approximately 70% yield from D-galactose.

Compound 1 is then converted into 1-S-acetyl-2,3,4,6-tetra-O-lauroyl-l-thio-#- D-galactopyranose, 2, by reaction of 1 with an excess of thiolacetic acid. In one embodiment, this reaction is conducted in the presence of an excess of boron trifluoride etherate, preferably using about 15 to 20 equivalents of boron trifluoride etherate based on 1, in an inert diluent, such as dichloromethane and the like.

Typically, this reaction is conducted initially at about 0°C and then at about 20°C to about 30*C for about 0.5 to about 48 hours.

c WO 98/22487 PCT/CA97/00866 In another embodiment, compound 2 can be prepared from 1 by contacting 1 with at least one equivalent;-preferably 1 to 1.2 equivalents, of benzylamine to selectively remove the 1-lauroyl group. This reaction is typically conducted at about to about 30 0 C for about 1 to about 96 hours to provide for 2,3,4,6-tetra-Olauroyl-(a,3)-galactopyranoside. This intermediate is then converted into an O- (2,3,4,6-tetra-0-lauroyl-(ca,1)-galactopyranosyl) trichloroacetimidate intermediate by contacting the tetralauroyl compound with an excess of trichloroacetonitrile, preferably about 10 equivalents, and about 0.8 to about 1.0 equivalents, of 1,8diazabicyclo[5.4.0]undec-7-ene (DBU) in an inert diluent, such as dichloromethane.

The resulting-O-trichloroacetidate intermediate is then contacted with an excess of thiolacetic acid in an inert diluent, such as dichloromethane, at about 25°C to about for about 1 to about 96 hours to provide for 1-S-acetyl-2,3,4,6-tetra-O-lauroyl- 1-thio-3-D-galactopyranose, 2.

In still another embodiment, compound 2 can be prepared by contacting compound 1 with about 1.5 to about 2.0 equivalents of thiolacetic acid and about equivalents of trimethylsilyl trifluoromethanesulfonate based on 1 in an inert diluent, such as dichloromethane and the like. Typically, this reaction is conducted initially at about 0°C and then at about 20°C to about 30 0 C for about 0.5 to about 72 hours.

This method is especially preferred since it provides the highest yield of compound 2 and produces no detectable traces of the corresponding a-isomer.

If desired, however, the a-isomer, 1-S-acetyl-2,3,4,6-tetra-O-lauroyl-1thio-a-D-galactopyranose, can be readily prepared by contacting compound 1 with an excess, preferably about 20 equivalents, of thioacetic acid in the presence of about to 1.1 equivalents of tin (IV) chloride in an inert diluent, such toluene, at ambient temperature for about 0.5 to about 2 hours. Alternatively, treatment of compound 1 with an excess, preferably about 3 to about 6 equivalents, of thioacetic acid in the presence of about 2.0 to 3.0 equivalents of trimethylsilyl trifluoromethanesulfonate in an inert diluent, such dichloromethane, at ambient temperature for about 12 to about 48 hours affords 1-S-acetyl-2,3,4,6-tetra-0-lauroyl-1-thio-a-D-galactopyranose.- WO 98/22487 PCT/CA97/00866 -26- The Michael addition of compound 2 to cyclohept-2-en-l-one then affords cycloheptanon-3-yl 2,3,4,6-tetra-O-lauroyl-l-thio--D-galactopyranoside, 3. This reaction is typically conducted by contacting 2 with at least one equivalent, preferably to 1.2 equivalents, of cyclohep-2-en-l-one in the presence of a molar excess of a dialkylamine, such as diethylamine.

Without being limited by any theory, it is believed that the dialkylamine first reacts with the thioacetyl of compound 2 thereby forming in situ the thiol derivative of compound 2 which then reacts under basic conditions generated by the dialkylamine with a Michael adduct.

Typically, this reaction is conducted in an inert diluent, such as -dichloromethane, at a temperature of from about -40*C to about 50 0 C for about 1 to about 6 hours.

The carbonyl group of compound 3 can then reduced using a reducing agent to provide for 3-hydroxycycloheptyl 2,3,4,6-tetra-O-lauroyl- -thio-f-Dgalactopyranoside, 4. Preferably, this reduction is conducted by contacting 3 with sodium borohydride, preferably about 1.2 to about 2.0 equivalents of sodium borohydride based on 3. Generally, this reaction is conducted in an inert diluent, such as tetrahydrofuran, isopropanol and mixture thereof, at a temperature of about to about 30*C for about 0.5 to about 3.0 hours. The resulting alcohol, 4, is readily purified by solid-phase extraction on C18 silica gel using pentane as an eluent.

Removal of the lauroyl groups from alcohol 4 is then accomplished by treating 4 with an excess of sodium methoxide in methanol and an inert diluent, such as dichloromethane, at about 25°C to about 30*C for about 1 to about 24 hours.

Neutralization of the reaction mixture with Amberlite IR-50S (H resin then provides for-3-hydroxycycloheptyl 1-thio-1-galactopyranoside, Alternatively, compound 3 can be reductively aminated to provide for 3aminocycloheptyl 2,3,4,6-tetra-O-lauroyl-l-thio--D-galactopyranoside, 5. In one embodiment of this reaction, compound 3 is contacted with an excess of ammonium acetate and at least one equivalent of sodium cyanoborohydride based on 3. This reaction is typically conducted in an inert diluent, such as methanol, tetrahydrofuran WO 98/22487 PCT/CA97/00866 -27and mixtures thereof, at a temperature of about 25°C to about 30 0 C for about 1 to about 72 hours.

In another preferred embodiment, the reductive amination reaction is accomplished by contacting compound 3 with an excess of ammonium acetate and an excess of trimethyl orthoformate based on 3, in an inert diluent, such as 1,2dichloroethane at a temperature of about 25 0 C to about 30 0 C for about 12 to about 72 hours to form an imine intermediate. The imine intermediate is generally not isolated but is contacted in situ with an excess of sodium borohydride, preferably about 1.2 to about 1.5 equivalents of sodium borohydride, based on 3. The resulting amino compound 5 is then readily purified by solid-phase extraction on C18 silica gel using pentane as an eluent.

Optionally, the amine group formed by reductive amination can be acylated with conventional acylating agents under conventional conditions. The acylating agent is generally of the formula L-C(O)R 6 where Lis a leaving group such as a halide, an activated ester, and the like.

The lauroyl groups are removed from compound 5 by contacting 5 with an excess of sodium methoxide in methanol and an inert diluent, such as dichlbromethane, at about 25*C to about 30 0 C for about 1 to about 24 hours.

Neutralization of the reaction mixture with Amberlite IR-50S (H resin then provides for 3 -aminocycloheptyl 1-thio--galactopyranoside, In one example, the primary amine group of compound B5 can optionally be acylated by contacting B5 with an excess of acetic anhydride in methanol containing a trace of water. Generally, this reaction is conducted at about 25"C to about 30*C for about 2 to about 24 hours to provide for 3-acetamidocycloheptyl 1-thio-3galactopyranoside, Alternatively, the primary amine group of 5 can be acylated with phthalic anhydride before removal of the lauroyl groups to provide for 3-(2carboxybenzamido)cycloheptyl 2,3,4,6-tetra-O-lauroyl-1 -thio-B-D-galactopyranoside, 6. This reaction is typically conducted by contacting compound 5 with at least one molar equivalent, preferably with an excess of phthalic anhydride. Preferably, this reaction is conducted in dry pyridine containing a catalytic amount of 4-(N,N- WO 98/22487 PCT/CA97/00866 -28dimethylamino)pyridine. The reaction is typically conducted at about 25C to about 0 C for about 12 to about 48 hours to provide for compound, 6. Removal of the lauroyl groups from 6 is then accomplished by treating 6 with sodium methoxide in methanol and an inert diluent, such as dichloromethane, at about 25*C to about for about 1 to about 24 hours. Neutralization of the reaction mixture with Amberlite resin then provides for 3-( 2 -carboxybenzamido)cycloheptyl 1-thio-O-Dgalactopyranoside, As shown in Figure 1, compound 3 can also be reductively aminated with an amino acid ester to provide for intermediates 7 or 8. Specifically, compound 3 is contacted with a molar excess of 3-alanine ter-butyl ester, preferably with equivalents based on 3, in the presence of at least one molar equivalent, preferably about 1.0 to about 1.2 equivalents, of sodium cyanoborohydride. Typically, this reaction is conducted in an essentially anhydrous inert diluent, such as acetonitrile, at a temperature of about 25 C to about 30 0 C for about 1 to about 72 hours. The resulting intermediate 7 is readily purified by solid-phase extraction on C18 silica gel using pentane as the eluent.

The tert-butyl ester group of compound 7 is readily hydrolyzed to the corresponding carboxylic acid by treating 7 with an excess of trifluoroacetic acid in an inert diluent such as dichloromethane. This reaction is typically conducted at about 25*C to about 30 0 C for about 1 to about 10 hours. The lauroyl groups of the resulting carboxylic acid intermediate are then removed using sodium methoxide in methanol as described above to provide for NO-[l-(l-thio-O-Dgalactopyranosyl)cyclohept-3-yl]-8-alanine, In a similar manner, compound 3 can be reductively aminated using other amino acid esters, such as glycine tert-butyl ester, L-leucine tert-butyl ester, Lhistidine methyl ester, L-tryptophan methyl ester, and L-arginine methyl ester, to provide for intermediate 8. In those cases where the amino acid ester employed is a ten-butyl ester, the tert-butyl ester is cleaved as described above using tiifluoroacetic acid to afford Noa-[1-(1-thio-0-D-galactopyranosyl)cyclohept-3-yl]-glycine, E5, and Na-[1-(1-thio--D-galactopyranosyl)cyclohept-3-yl]-L-leucine, G5. Alternatively, in WO 98/22487 PCT/CA97/00866 -29those cases where an amino acid methyl ester is employed, the lauroyl groups of intermediate 8 are preferably removed before cleaving the methyl ester by treatment of 8 with sodium methoxide in methanol as described above. Subsequently, the methyl ester of the amino acid moiety is cleaved to the corresponding carboxylic acid by treatment with an excess of aqueous lithium hydroxide for about 0.5 to about 2 hours. Neutralization of the reaction mixture with Amberlite IR-50S (H resin then provides for Nc-[l-(-thio-5-D-galactopyranosyl)cyclohept-3-yl]-L-histidine,H5, Na- [1-(1-thio-/-D-galactopyranosyl)cyclohept-3-yl]-L-tryptophan, 15, and Na-[1-(1-thio-B- D-galactopyranosyl)cyclohept-3-yl]-L-arginine, Additionally, if desired, the hydroxyl group of alcohol derivatives, such as compound 4, can be converted into a leaving group, such as the mesylate, tosylate, etc., and displaced with various nucleophiles. For example, treatment of an alcohol derivative with an excess, preferably about 1.1 to about 1.5 equivalents, of methanesulfonyl chloride in pyridine and an inert diluent, such as THF, affords the corresponding mesylate. The mesylate group can then be displaced with, for example, sodium azide to provide the corresponding azido derivative. This reaction is typically conducted by contacting the mesylate compound with an excess, preferably about 5 to about 50 equivalents of sodium azide in an inert diluent, such as N,Ndimethylformamide, THF and mixtures thereof, at a temperature of from about to about 100*C for about 1 to about 6 hours. Preferably, a crown ether, such as 18crown-6, is added to the reaction mixture to promote the displacement reaction.

The azido derivative can then be reduced with a reducing agent to afford the corresponding primary amine, a compound such as 5. Preferably, this reaction is conducted by contacting the azido compound with about 1.0 to about 1.1 equivalents of sodium borohydride and about 2.0 to about 2.2 equivalents of-fiickel chloride (NiC1 2 in an inert diluent, such as ethanol, isopropanol, or mixtures thereof, at a temperature of from about 0°C to about 50 0 C for about 0.5 to about 6 hours.

Removal of the lauroyl protecting groups can then be accomplished using the procedures described above.

Additionally, the primary amine group of amino compounds such as 5 can. be further derivatized by reductive alkylation to afford a secondary amine. Typically, WO 98/22487 PCT/CA97/00866 this reaction is conducted by contacting the primary amine with an excess, preferably about 2 to about 500 equivalents of an aldehyde or a ketone in the presence of at least one equivalent, preferably about 1.0 to about 10 equivalents, of a reducing agent, such as sodium triacetoxyborohydride. This reaction is typically conducted in an inert- 5 diluent, such as dichloromethane, methanol, or mixtures thereof, at a temperature of about 0*C to about 50°C for about 10 to about 48 hours. In a preferred embodiment, the ketone employed in this reaction is a cyclic ketone including, by way of example, cyclobutanones, such as 3 ,3-dimethylcyclobutan-l-one; cyclopentanones, such as 3,3dimethylcyclopentan-1-one; cyclohexanones and cycloheptanones.

The lauroyl groups of the resulting secondary amine are then removed by contacting the lauroyl-protected compound with an excess of sodium methoxide in methanol and an inert diluent, such as dichloromethane, at about 25*C to about for about 1 to about 24 hours. Neutralization of the reaction mixture with Amberlite (H resin then provides the desired secondary amine compound.

As noted above, Figure 2 illustrates the synthesis of various 1-thiogalactose derivatives using an a-halocarbonyl carbonyl compound, 2-chlorocyclohexanone.

As shown in Figure 2, 1-S-acetyl-2,3,4,6-tetra-O-lauroyl-1-thio-O-D-galactopyranose, 2, prepared as described above, reacts with 2-chlorocyclohexanone to give cyclohexanon-2-yl 2,3,4,6-tetra-O-lauroyl-l-thio-1-D-galactopyranoside, 9. This reaction is typically conducted by contacting 2 with at least one equivalent, preferably to 1.2 equivalents, of 2-chlorocyclohexanone in the presence of an excess of a dialkylamine, such as diethylamine. Typically, this reaction is conducted in an inert diluent, such as dichloromethane, at a temperature of from about -40*C to about for about 1 to about 6 hours to afford compound 9.

Compound 9 can then be reacted using the same reagents and conditions described above for compound 3 to afford various 1-thiogalactose derivatives.

Specifically, compound 9 is reduced with sodium borohydride to provide 10 which, after removal of the lauroyl groups, affords 2-hydroxycyclohexyl 1-thio--Dgalactopyranoside, A2.

Alternatively, compound 9 is reductively aminated with ammonium acetate and sodium cyanoborohydride to provide for intermediate 11 which, upon removal of the WO 98/22487 PCT/CA97/00866 -31lauroyl groups, affords 2-aminocyclohexyl l-thio--D-galactopyranoside, B2.

Compound B2 can then be acylated with acetic anhydride to give 2acetamidocyclohexyl 1-thio-1-D-galactopyranoside, C2. Alternatively, intermediate 11 can be acylated with phthalic anhydride to provide for intermediate 12 which affords 2 2 -carboxybenzamidocyclohexyl 1-thio--D-galactopyranoside, D2, by removal of the lauroyl groups using the conditions described above.

Additionally, compound 9 can be reductively aminated using an 0-alanine tertnbutyl ester to provide for intermediate 13 which then affords N-[1-(1-thio-0-Dgalactopyranosyl)cyclohex-2-yl]-3-alanine, F2, upon deprotection. Alternatively, compound 9 can be reductive aminated with other amino acid esters, such as glycine tert-butyl ester, L-leucine tert-butyl ester, L-histidine methyl ester, L-tryptophan methyl ester, and L-arginine methyl ester, to provide intermediate 14 which upon deprotection, affords Nce-[1-(1-thio-f-D-galactopyranosyl)cyclohex-2-yl]-glycineE2, No-[1-(1-thio-/-D-galactopyranosyl)cycloex-2-yl]-L-leucine G2, Na-[1-(1-thio-fl-Dgalactopyranosyl)cyclohex-2-yl]-L-histidine 112, Na-[1-(1 -thio-1-Dgalactopyranosyl)cyclohex-2-yl]-L-tryptophan 12, and Nat-[1-(1-thio-fl-Dgalactopyranosyl)cyclohex-2-yl]-L-arginine J2.

Optionally, the saccharide derivatives of formula I wherein Y is a sulfide linking group can be oxidized using conventional reagents and conditions to provide the corresponding sulfoxide (Y and sulfone (Y -SO 2 derivatives. Suitable reagents for oxidizing a sulfide compound to a sulfoxide include, by way of example, hydrogen peroxide, peracids such as 3chloroperoxybenzoic acid (MCPBA), sodium periodate, sodium chlorite, sodium hypochlorite, calcium hypochlorite, tert-butyl hypochlorite and the like. Chiral oxidizing reagents (optically active reagents) may also be employed to provide chiral sulfoxides. Such optically active reagents are well known in the art and include, for example, the reagents described in Kagen et al." and references cited therein.

The oxidation reaction is typically conducted by contacting the saccharide derivative with about 0.95 to about 1.1 equivalents of the oxidizing reagent in an inert diluent, such as dichloromethane, at a temperature ranging from about 0*C to about WO 98/22487 PCT/CA97/00866 -32for about 1 to about 48 hours. The resulting sulfoxide can then be further oxidized to the corresponding-sulfone by contacting the sulfoxide with at least one additional equivalent of an oxidizing reagent, such as hydrogen peroxide, MCPBA, potassium permanganate and the like. Alternatively, the sulfone can be prepared directly by contacting the sulfide with at least two equivalents, and preferably an excess, of the oxidizing reagent.

In a similar manner, the saccharide of formula I, wherein R 4 is -XR 5 X is sulfur and R 5 is a defined substituent other than hydrogen, can be oxidized to afford the corresponding sulfoxide (X and sulfone (X -SO 2 derivatives.

Additionally, if desired, the hydroxyl groups of the saccharide moiety may be readily acylated, sulfonylated or phosphorylated using art recognized procedures and reagents to provide compounds of formula I wherein at least one of the hydroxyl groups of the saccharide is -O-SO 2 -OH, -C(O)R' 2 or pharmaceutically acceptable salts thereof, where R' 0 and R" are as defined above. Such acylation reactions may occur as an initial step of the synthesis using an acyl halide, such as lauroyl chloride, as described above) or as a post-synthetic transformation of compounds of formula I using, for example, acyl halides, anhydrides, halophosphates, sulfur trioxide, and the like.

For example, a de-blocked hydroxyl group can be sulfonylated by treating the hydroxy-containing compound with an excess, preferably about 1.1 to about 1.2 equivalents, of a pyridine:sulfur trioxide complex in an inert diluent, such as N,Ndimethylformamide, at ambient temperature for about 1 to about 24 hours. Typically, the resulting sulfate -O-SO 2 -OH) is isolated as its salt by treatment with, for example, a Na' resin in an inert diluent, such as methanol. Further reaction conditions suitable for forming sulfates and phosphates can be found, for example, in U.S. Patent No. 5,580,85812 The methods illustrated in Figures 1 and 2 were conducted in a solution phase.

Surprisingly, these methods can also be conducted on the solid phase using reaction conditions similar to those described above for the solution phase. When conducted on the solid phase, one of the reagents employed is attached to a solid support via a WO 98/22487 PCT/CA97/00866 -33cleavable or non-cleavable linking arm. Such linking arms are well known in the art as well as their attachment to either the thiosaccharide or the coupling reagent.

Either of the reagents can be attached to the solid support without criticality provided that the attachment does not alter the reactivity of the reagent. For example, a linking arm may. be covalently attached to any position of the thiosaccharide other than the thiol group. Such attachments are preferably made through, for example, an ester or ether linkage to one the hydroxyl group of the thiosaccharide. A preferred linking arm is derived from succinic acid.

By way of example, 1-dithioethyl--D-galactopyranoside is readily attached to a trityl chloride resin having about 0.80 to about 1.00 mmol/g of active chlorine by contacting the resin with about 0.75 to about 2.0 equivalents of 1-dithioethyl--Dgalactopyranoside in pyridine containing a catalytic amount of 4-(N,Ndimethylamino)pyridine at a temperature ranging from about 25"C to about 100*C for about 12 to 48 hours. A free thiol group at the 1-position of the covalently bound galactose is then generated by treating the resin with dithiothreitol (Cleland's reagent) and triethylamine in an inert diluent, such as methanol, for about 6 to 24 hours at ambient temperature. The resulting 1-thio-3-D-galactopyranoside is then reacted as described above to afford a 1-thiogalactose derivative of formula I covalently attached to the solid support resin. If desired, the 1-thiogalactose derivative can be cleaved from the solid support resin by contacting the resin with an excess of trifluoroacetic acid and triisopropylsilane in an inert diluent, such as dichloromethane, at ambient temperature.

Similarly, a linking arm can be covalently attached to any position of the coupling reagent provided that the point of attachment does not interfere with the -ichael addition of the thiosaccharide to the a,o-unsaturated carbonyl group or with the displacement of the halide from the a-halocarbonyl compound by the thiosaccharide. Accordingly, the linking arm is preferably attached to the coupling reagent through any one of substituents R'-R via a covalent bond. Such linkage can be through, for example, an ester, ether, amine, amide, or urea functional group and the like.

WO 98/22487 PCT/CA97/00866 -34- By way of example, a carboxylic acid moiety can be covalently attached to an aminated solid support using conventional coupling procedures and reagents.

Typically, such a coupling reaction will be conducted using well-known coupling reagents such as carbodiimides, BOP reagent (benzotriazol-1-yloxytris(dimethylamino)phosphonium hexafluorophosphonate) and the like. Suitable carbodiimides include, by way of example, dicyclohexylcarbodiimide

(DCC),

diisopropylcarbodiimide, 1-( 3 -dimethylaminopropyl)-3-ethylcarbodiimide (EDC) and the like. Preferably, a well-known coupling promoter, such as N-hydroxysuccinimide, 1-hydroxybenzotriazole and the like, is also employed in the reaction mixture to facilitate the coupling reaction.

The coupling reaction is typically conducted by contacting the solid support with an excess, preferably about 1.1 to about 10 or more equivalents, of the carboxylic acid-containing compound (based on the number of equivalents of amino groups present on the solid support) and at least one equivalent, preferably about to about 3.0 equivalents, of the coupling reagent (based on the carboxylic acid groups) in an inert diluent, such N,N-dimethylformamide and the like. If desired, least one equivalent, preferably about 1.5 to about 3.0 equivalents (based on the 1-thiogalactose derivative), of a coupling promoter such as 1-hydroxybenzotriazole may also be used in the reaction. Generally, the coupling reaction is conducted at a temperature ranging from about 0°C to about 50°C for about 24 to about 100 hours. Upon completion of the reaction, the solid support is preferably contacted with excess acetic anhydride in methanol at a temperature ranging from about 0°C to about 40*C for about 12 to about 24 hours to cap any unreacted amino groups present on the solid support. The yield of incorporation of a thiosaccharide onto the solid support can be determined using well-established procedures such as those described, for example, by M. Dubois et al.

3 2. Method for Preparing A Thiosaccharide Derivative Library In another aspect, the methods of this invention provide for a thiosaccharide derivative library. Such libraries are produced by synthesizing on each of a plurality WO 98/22487 PCT/CA97/00866 of solid supports a single compound wherein each compound comprises a thiosaccharide derivative.

The thiosaccharide derivative libraries provided by this invention are synthesized by first apportioning solid supports among a plurality of reaction vessels.

Such supports comprise a reactive functional group capable of covalently binding to the solid support. The functional group is one that is capable of covalently binding a thiosaccharide at a position other than the thiol group. Suitable functional groups include, by way of example, alcohols, amines, isocyanates, carboxylic acid groups, esters and the like. In one embodiment, this is accomplished by selectively blocking the thiol group with a removable blocking group which, after coupling of the thiosaccharide to the solid support, is removed thereby freeing the thiol group for further reaction.

The supports in each reaction vessel are then contacted with aunique thiosaccharide under conditions wherein the thiosaccharide is covalently attached to the solid supports through the reactive functional group. This reaction is typically conducted by contacting the solid support with at least one equivalent, preferably 1 to equivalents, of the thiosaccharide based on the functional groups on the solid support.

After attaching the thiosaccharide to the solid support, the supports are then pooled and the pooled supports are then apportioned among a plurality of reaction vessels.

The supports having a thiosaccharide covalently attached thereto are then contacted in each reaction vessel with a unique coupling reagent selected from the group consisting of Michael acceptors and a-halocarbonyl compounds to provide for a thiosaccharide carbonyl compound which covalently bound to the support. This reaction is preferably conducted as described above.

The thiosaccharide carbonyl compound is then reduced as described above to provide for an alcohol and/or an amine derivative. Optionally, the hydroxy or-amino group of these compounds can be further derivatized as described above to form a group selected from esters, substituted amines, amides, carbamates, ureas, thioesters and thiocarbamates.

WO 98/22487 PCT/CA97/00866 -36- In an alternative embodiment, the thiosaccharide derivative libraries provide by this invention are synthesized by first apportioning solid supports among a plurality of reaction vessels wherein such supports comprise a reactive functional group covalently bound to the solid support such that the functional group one that is capable of covalently binding a coupling reagent. Such functional groups include, by way of example, alcohols, amines, isocyanates, carboxylic acid groups, esters and the like.

The supports in each reaction vessel is then contacted with a unique coupling reagent selected from the group consisting of Michael acceptors and a-halocarbonyl compounds under conditions wherein the coupling reagent is covalently attached to the solid supports through the reactive functional group. Typically, this reaction is conducted by contacting the solid support with at least one equivalent of the coupling reagent, preferably with about 1 to about 5 equivalents, based on the functioinal groups on the solid support.

After attaching the coupling reagent to the solid support, the supports are then pooled and the pooled supports are then apportioned among a plurality of reaction Svessels.

The supports having a coupling reagent covalently attached thereto are then contacted in each reaction vessel with a unique thiosaccharide to provide for a thiosaccharide carbonyl compound which is covalently bound to the support. This reaction is preferably conducted as described above. The thiosaccharide carbonyl compounds can then be reduced to provide for a plurality of alcohol and/or amine derivatives. As above, these alcohol and/or amine derivatives can optionally be further derivatized to provide for a group selected from esters, substituted amines, amides, carbamates, ureas, thioesters, and thiocarbamates.

In a preferred embodiment, an identifier tag is employed in the methods of this invention. The identifier tag has a recognizable feature that is, for example, microscopically or otherwise distinguishable in shape, size, mass, charge, or color.

This recognizable feature may arise from the optical, chemical, electronic, or magnetic properties of the tag, or from some combination of such properties. In essence, the tag serves to label a molecule and to encode information decipherable at the level of one (or a few) molecules or solid supports. By using identifier tags to WO 98/22487 PCT/CA97/00866 -37track the synthesis pathway that each member of a chemical library has taken, one can deduce the structure of any chemical in the library by reading the identifier tag.

The identifier tags identify each reagent or other reaction step that an individual library member or solid support has experienced and record the step in the synthesis series in which each reagent was added or other chemical reaction performed. The tags may be attached immediately before, during, or after the reagent addition or other reaction, as convenient and compatible with the type of identifier tag, modes of attachment, and chemistry of activated ketone or other molecular synthesis. The identifier tag can be associated with the thiosaccharide derivatives through a variety of mechanisms, either directly, through a linking molecule, or through a solid support upon which the thiosaccharide derivative is synthesized. In the latter mode, one could also attach the tag to another solid support that, in turn, is bound to the solid support upon which the thiosaccharide derivative is synthesized.

The identifier tag is added when the solid supports that have undergone a specific reagent addition or other chemical reaction step are physically together and so can be tagged as a group, prior to the next pooling step. Preferred identifier tags include, by way of example, peptides' 4 1 5 oligonucleotides 1 6 and halocarbon derivatives 17 3. Screening of Thiosaccharide Derivative Libraries The libraries of thiosaccharide derivatives compounds of formula I) may be screened for biological activity. Generally the library to be screen is exposed to a biological substance, usually a protein such as a receptor, enzyme, membrane binding protein or antibody, and the presence or absence of an interaction between the thiosaccharide derivative and the biological substance is determined. Typically this will comprise determining whether the biological substance is bound to one or more of the members of the library. Such binding may be determined by attaching a label to the biological substance. Commonly used labels include fluorescent labels. Other methods of labeling may be used, such as radioactive labels. The degree of binding affinity may be determined by quantitating the amount or intensity of the bound label.

Wo 98/22487 PCT/CA97/00866 -38- Thus, various lead compounds may be selected by identifying which compounds bind the particular biological substance most effectively.

In a preferred embodiment, bead-based libraries are screened by assays in which each different molecule in the library is assayed for its ability to bind to a receptor of interest. The receptor is contacted with the library of thiosaccharide derivatives, forming a bound member between the receptor and any thiosaccharide derivative in the library able to bind the receptor under the assay conditions. The bound thiosaccharide derivative is then identified by examination of the tag associated with that thiosaccharide derivative. The receptor to which the library is exposed under binding conditions can be a mixture of receptors, each of which is associated with an identifier tag specifying the receptor type, and consequently two tags are examined after the binding assay. Specific beads can be isolated in a receptor screening by a number of means, including infinite dilution, micromanipulation, or preferably, flow cytometry fluorescence activated cell sorting (FACS)). By adopting cell-sized solid supports or beads, one can use flow cytometry for high sensitivity receptor binding analysis and facile bead manipulation.

Thiosaccharide derivatives can be synthesized on beads and cleaved prior to assay. Cleavage of the thiosaccharide derivatives from the beads may be accomplished cleavable linker arms which are cleaved using conventional methods. In either event, the thiosaccharide derivatives of interest are cleaved from the beads but remain contained within the compartment -along with the bead and the identifier tag(s).

Soluble tagged thiosaccharide derivatives can also be screened using an immobilized receptor. After contacting the tagged thiosaccharide derivatives with the immobilized receptor and washing away non-specifically bound molecules, bound, tagged thiosaccharide derivatives are released from the receptor by any of a wide variety of methods. The tags are optionally amplified and then examined and decoded to identify the structure of the molecules that bind specifically to the receptor. A tagged thiosaccharide derivative in solution can be assayed using a receptor immobilized by attachment to a bead, for example, by a competition assay with a fluorescently labeled ligand. One may recover the beads bearing immobilized receptors and sort the beads using FACS to identify positives (diminished fluorescence WO 98/22487 PCT/CA97/00866 -39caused by the library molecule competing with the labeled ligand). The associated identifier tag is then-anplified and decoded.

Preferably, the libraries described herein will contain at least about 2 compounds, more preferably at least about 102 compounds, still more preferably from about 102 to about 1010 compounds and even still more preferably from about 10' to about 106 compounds.

Of particular interest is the identification of thiosaccharide derivatives which block binding of a toxin, such as heat-labile enterotoxin or cholera toxin, the toxin's receptor either in vitro or in vivo, and compounds which inhibit binding of organisms bacteria, virus, fungi, and the like), including enterovirulent organism such as Vibrio cholerae and enterotoxigenic strains of Escherichia coli, to their cell surface receptors.

The following synthetic and biological examples are offered to illustrate this invention and are not to be construed in any way as limiting the scope of this invention. Unless otherwise stated, all temperatures are in degrees Celsius.

EXAMPLES

In the examples below, the following abbreviations have the following meanings. If an abbreviation is not defined, it has its generally accepted meaning.

A angstroms bd broad doublet bs broad singlet d doublet dd doublet of doublets DMAP dimethylaminopyridine eq. equivalents g grams L liter m multiplet meq milliequivalent mg milligram mL milliliter mmol millimol N normal q quartet quint. quintet WO 98/22487 PCT/CA97/00866 s singlet t triplet TFA trifluoroacetic acid THF tetrahydrofuran TLC thin layer chromatography pL microliter 'H-Nmr spectra were recorded with a Brueker AM-360 spectrometer and MALDI-TOF mass spectra were recorded with a HP G2020A (LD-TOF) instrument.

Optical rotations were measured with a Perkin-Elmer 241 polarimeter. Reactions were monitored by TLC on Silica Gel FG254 Merck, Darmstadt, Germany).

Example A Solid-Phase Extraction of Lauroylated Intermedia7fes As indicated in the following examples, certain lauroylated reaction intermediates were purified by solid-phase extraction. In this purification procedure, the reaction mixture is concentrated, re-dissolved in methanol, and applied onto C18 silica (Waters Prep C18, 125 A, 1 g per 20 mg lauroylated carbohydrate). The C18 silica is then washed with methanol (10 mL/ g C18 silica) and the product is eluted with pentane (10 mL/ g C18 silica). For L-arginine containing compounds, the reaction mixture is concentrated, re-dissolved in 70% methanol and applied onto C18 silica. The C18 silica is then washed with 70% methanol and the product is eluted with methanol. The resulting product contains no residual reagents as determined by TLC, 'H-nmr, or MALDI-TOF mass spectroscopy.

Example B Synthesis of 1.2.3.4.6-Penta-O-lauroyl-a-D-galactopyranose 1 To a suspension of galactose (3.78 g, 21.0 mmol), pyridine (50 mL), and 4dimethylaminopyridine (cat.) in pentane (150 mL) under argon atmosphere, was added lauroyl chloride (50 mL, 210 mmol) at -78 0 C. The mixture was allowed to reach ambient temperature. The resulting white slurry slowly dissolved and a fine precipitate of pyridinium hydrochloride formed. After 40 h, the pyridinium WO 98/22487 PCT/CA97/00866 -41hydrochloride was filtered off and the pentane solution was concentrated. Column chromatography (SiO 2 pentane/EtOAc 9:1) gave 1 (16.0 g, 70% yield), [a]D 25 +39" (c 0.9, CHCl 3 'H-Nmr data (CHCI 3 6 6.39 1H, J 2.4 Hz, 5.51 (br s, 1H, 5.35 2H, H-2 and 4.32 (br t, 1H, J 6.6 Hz, 4.08 2H, J 6.6 Hz, H-6a and H-6b), 2.39, 2.38, 2.30, 2.26 (4 t, 2H each, J 7.5 Hz, -CH 2

CO-),

2.21 2H, -CH 2 0.88 15 H, J 7.0 Hz, -CH3). Anal. Calcd for CH,220,: C, 72.2; H, 11.3. Found: C, 72.6; H, 11.5.

Example C Synthesis of 1-S-Acetyl-2.3.4.6-tetra-0-lauroyl- I -thio-B-D-galactopyranose (2) Method 1: To compound 1 (from Example B, 1 g, 0.91 mmol) and thiolacetic acid (0.4 mL, 9.1 mmol) in dry dichloromethane (5 mL) under argon at 0°C, was added boron trifluoride etherate (1.7 mL, 13.6 mmol). The cold-bath was removed after 10 min and after 24 h the mixture was diluted with dichloromethane, washed with saturated sodium bicarbonate, dried over sodium sulfate, and concentrated.

Column chromatography (SiO 2 pentane/Et20/EtOAc 9:1:1) gave 2 (0.60 g, yield).

Method 2: To compound 1 (from Example B, 276.5 mg, 0.253 mmol) in dry tetrahydrofuran (2.0 mL) under-argon, was added benzylamine (27.9 jiL, 0.255 mmol). The mixture was concentrated after 70 h. The residue was dissolved in dry dichloromethane (4.0 mL) under argon and then trichloroacetonitrile (250 iL, mmol) and 1,8-diazabicyclo[5.4.0]undec-7-ene (30 0.2 mmol) were added. The mixture was concentrated after 3 h and the residue was flashed through a short column (SiO 2 pentane/EtOAc 19:1), then concentrated. To the residue in dry dichloromethane (3.5 mL) under argon, was added thiolacetic acid (1 mL). After 96 h, the reaction mixture was concentrated and the residue was purified by column chromatography (SiO 2 pentane, EtOAc 19:1) to give 2 (90 mg, 37% yield), [a]D 2 21" (c 1, CHC1 3 'H-Nmr data (CHC1 3 6 5.47 1H, J 3.4 Hz, 5.32 1H, J 10.0 Hz, 5.25 1H, J 10.0 Hz, 5.12 (dd, 1H, J 3.4 and 10.0 Hz, H- WO 98/22487 PCT/CA97/00866 -42- 4.08 3H, H-5, H-6a and H-6b), 2.14-2.43 8H, -CH 2 2.37 3H, SAc), 0.88 15 H, J 7.0 Hz, -CH3). Anal. Calcd for CHo20S: C, 69.5; H, 10.6; S, 3.3. Found: C, 69.4; H, 10.8; S, Method 3: To compound 1 (20.0 g, 18.2 mmol) and thioacetic acid (5.0 mL, 1.9 eq.) in dry dichloromethane (300 mL) under argon, was added trimethylsilyl trifluoromethanesulfonate (5.0 mL, 0.5 eq.) at 0°C. The cold-bath was immediately removed and after 48 h the mixture was diluted with dichloromethane, washed with saturated sodium hydrogen carbonate, dried (Na 2

SO

4 and concentrated. Column chromatography (SiO 2 pentane/EtOAc 20:1) gave 2 (13.7 g, +21" (c 1, CHC1 3 'H-Nmr data (CHC1 3 5 5.47 1H, J 3.4 Hz, 5.32 1H, J 10.0 Hz, 5.25 1H, J 10.0 Hz, 5.12 (dd, 1H, J 3.4 and 10.0 Hz, H-3), 4.08 3H, H-5, H-6a and H-6b), 2.14-2.43 8H, -CH 2 2.37 3H, SAc), 0.88 15 H, J 7.0 Hz, -CH 3 Anal. Calcd for C 6

H

1 io20AS: C, 69.5; H, 10.6; S, 3.3. Found: C, 69.4; H, 10.8; S, Example C' Synthesis of 1-S-Acetvl-2.3.4.6-tetra-O-lauroyl-l-thio-ca-D-galactopyranose Method 1: To compound 1 (20.0 g, 18.2 mmol) and thioacetic acid (27.0 mL,' eq.) in dry toluene (80 mL) under argon was added tin (IV) chloride (21.3 mL) dropwise at room temperature. The reaction was monitored by Tic carefully. After 1 h, 600 mL of 1M aqueous HC1 was added to the vigorously stirred mixture and the resulting mixture was filtered through Celite to remove the emulsion of tin salts. The mixture was diluted with pentane (800 mL), washed with water (2 x 400 mL), saturated sodium hydrogen carbonate (300 mL) and water (300 mL), dried with Na 2

SO

4 and concentrated. The residue was purified by column chromatography three times (SiO 2 pentane/EtOAc 20:1, 30:1, 40:1) to give 1-S-acetyl-2,3,4,6-tetra-Olauroyl-l-thio-a-D-galactopyranose (3.65 g, 'H-Nmr data (CHC1 3 6 6.26 (d, 1H, J 5.5 Hz, 5.47 (dd, 1H, J 11.0 Hz, 5.5 Hz, 5.46 1H, J 3.5 Hz; WO 98/22487 PCT/CA97/00866 -43- 5.04 (dd, 1H, J 11.0 Hz, 3.5 Hz, 4.17 1H, J 6.5 Hz, 4.06 (d, 2H, J 6.5 Hz, H-6a and H-6b), 2.38 8H, J 7.0 Hz,

-COCH

2 2.40 3H, -SAc), 0.87 15H, J 7.0 Hz, -CH 3 Method 2: To compound 1 (25.0 g, 22.9 mmol) and thioacetic acid (8.5 mL, 114.5 mmol) in dry dichloromethane (100 mL) under argon, was added trimethylsilyl trifluoromethanesulfonate (5.6 mL, 45.8 mmol) at room temperature. After 20 h, the mixture was diluted with dichloromethane (600 mL), washed with saturated sodiumhydrogen carbonate (250 mL) and water (2 x 200 mL), dried with Na 2 SO, and concentrated. TheTesidue was purified by column chromatography three times (SiO 2 pentane/EtOAc 20:1, 30:1, 40:1) to give l-S-acetyl-2,3,4,6-tetra-O-lauroyl-l-thio-a- D-galactopyrahose (1.59 g, Example D General Procedure for Michael Additions and ct-Halocarbonyl Substitutions To compound 2 (1 mmol) and an electrophile (1.2 mmol) in dry dichloromethane (8 mL) under argon, was added Et 2 NH (4 mL). After 1-3 h, the mixture was concentrated and the residue was purified by column chromatography on SiO2 by eluting with pentane/EtOAc. The products were characterized with 1 H-nmr spectroscopy and MALDI-TOF mass spectroscopy.

Example E General Procedure for Reduction to Alcohols To the product from Example D (100 pmol) in dry tetrahydrofuran (2.0 mL) and isopropanol (0.7 mL) under argon atmosphere, was added NaBH 4 (150 /mol).

After 0.5-3 h, the mixture was concentrated (acetic acid (about 40 jL) was added prior to concentration in some cases) and the residue was purified according to the solid-phase extraction procedure of Example A. The product alcohols were characterized with 'H-nmr spectroscopy and MALDI-TOF mass spectroscopy.

WO 98/22487 PCT/CA97/00866 -44- Example F General Procedure for Reductive Amination to a Primary Amine Method 1: To the product from Example D (100 smol) and ammonium acetate mg, 1 mmol) in dry methanol (2.3 mL) and tetrahydrofuran (0.9 mL) under argon, was added NaCNBH 3 (100 mol). After 1-72 h, the mixture was concentrated and the residue purified according to the solid-phase extraction procedure of Example A. The product amines were characterized with 'H-nmr spectroscopy and MALDI- TOF mass spectroscopy.

Method 2: The product from Example D (200 mg, 0.198 mmol) and dry

NH

4 OAc (30 mg, 0.4 mmol) were stirred in dry MeOH (6 mL), dry 1,2dichloroethane (6 mL), and trimethyl orthoformate (1 mL) under argon for 24 h (until TLC analysis showed that most of the starting material was consumed). NaBH 4 mg, 0.26 mmol) was added and after 1 h the mixture was concentrated. The residue was purified according to the solid-phase extraction procedure of Example A to provide the primary amine (containing traces of the corresponding alcohol). This mixture was dissolved in pentane/EtOAc and applied onto a Waters Sep-Pak Plus Longbody SiO 2 cartridge. The cartridge was washed with pentane/EtOAc (1:1, mL) (to remove the corresponding alcohol), followed by elution with toluene/EtOH 30 mL) to afford the primary amine.

Example G General Procedure for Acylation of Primary Amines with Phthalic Anhydride The O-lauroylated primary amine from Example F (100 1mol), phthalic anhydride (2.7 mmol), and 4-(N,N-dimethylamino)pyridine (catalytic) were dissolved in dry pyridine. The mixture was concentrated after 12-48 h and the residue purified according to the solid-phase extraction procedure of Example A. The product 2carboxybenzamides were characterized with 'H-nmr spectroscopy and MALDI-TOF mass spectroscopy.

o I WO 98/22487 PCT/CA97/00866 Example H General Procedure for Reductive Amination with Amino Acids To the product from Example D (100 ;mol) and an amino acid ter-butyl ester hydrochloride or methyl ester hydrochloride (1 mmol) in dry MeCN (2.25 mL) and THF (0.75 mL), was added NaCNBH 3 (100 /mol). After 1-72 h, the mixture was concentrated and the residue was purified according to the solid-phase extraction procedure of Example A. The product N-alkylated amino acids were characterized with 'H-nmr spectroscopy and MALDI-TOF mass spectroscopy. Example I General Procedure for Deblocking of Alcohols To the lauroylated alcohol from Example E (100 /1mol) in dry methanol (7.1 mL) and dichloromethane (1.4 mL) under argon atmosphere, was added methanolic sodium methoxide (1 M, 50 After 1-24 h, the mixture was neutralized with Amberlite IR-50S (H resin, filtered and concentrated. The residue was dissolved in water and applied onto a column of C18 silica (Waters Prep C18, 125 A, 5 The C18 silica was washed with water (50 mL), and the product was then eluted with methanol (50 mL). The resulting alcohols were characterized with 'H-nmr spectroscopy and MALDI-TOF mass spectroscopy.

Example J General Procedure for Deblocking of Primary Amines To the O-lauroylated primary amine from Example F (100 1mol) in dry methanol (7.1 mL) and dichloromethane (1.4 mL) under argon, was added methanolic sodium methoxide (1 M, 50 juL). After 1-24 h, the mixture was neutralized with Amberlite IR-50S (H resin, filtered and concentrated. The residue was dissolved in dichloromethane/methanol 2:1 and applied to a Waters SepPak Plus Longbody SiO 2 cartridge. The cartridge was washed with dichloromethane/methanol and then the product was eluted with dichloromethane/methanol/water (20 mL) and concentrated. The residue was dissolved in water and applied onto a column of C18 WO 98/22487 PCT/CA97/00866 -46silica (Waters Prep C18, 125 A, 5 The C18 silica was washed with water mL) and then the productwas-eluted with methanol (50 mL). The resulting primary amines were characterized- with 1H-nmr spectroscopy and MALDI-TOF mass spectroscopy.

Example K General Procedure for N-Acetylation of Primary Amines To the primary amine from Example J (100 pmol) in moist methanol (4.4 mL) was added acetic anhydride (0.4 mL). The mixture was concentrated after 2-24 h, redissolved in water and applied to a column of C18 silica (Waters Prep C18, 125 A, The C18 silica was washed with water (50 mL) and then the product was eluted with methanol (50 mL). The resulting acetamides were characterized with 'H-nmr spectroscopy and MALDI-TOF mass spectroscopy.

Example L General Procedure for Deblocking of 2-Carboxybenzamides To the O-lauroylated 2-carboxybenzamide from Example G (100 tmol) in dry methanol (7.1 mL) and dichloromethane (1.4 mL) under argon, was added methanolic sodium methoxide (1 M, 50 1L). After 1-24 h, the mixture was neutralized with Amberlite IR-50S resin, filtered and concentrated. The residue was dissolved in dichloromethane/methanol and applied to a Waters SepPak Plus Longbody SiOz cartridge. The cartridge was washed with dichloromethane/methanol and then the product was eluted with dichloromethane/methanol/water (65:35:5) (20 mL) and concentrated. The residue was dissolved in water and applied to a column of C18 silica (Waters Prep C18, 125 A, 5 The C18 silica was washed with water mL), and then the product was eluted with methanol (50 mL). The resulting 2carboxybenzamides were characterized with 'H-nmr spectroscopy and MALDI-TOF mass spectroscopy.

WO 98/22487 PCT/CA97/00866 -47- Example M General Procedure for Deblocking of N-Alkvlated Glycine. -Alanine. and L-Leucine Compounds The N-alkylated amino acid tert-butyl ester from Example H (100 pmol) was treated with trifluoroacetic acid (3.5 mL) in dry dichloromethane (3.5 mL) for 1-10 h.

n-Propyl acetate (8 mL) and toluene (16 mL) were added and the mixture was concentrated, then co-concentrated twice with toluene. To the residue in dry methanol (7.1 mL) and dichloromethane (1.1 mL) under an argon atmosphere was added methanolic sodium methoxide (1 M, 200 tL). After 1-24 h, the mixture was neutralized with Amberlite IR-50S resin, filtered and concentrated. The residue was dissolved in dichloromethane/methanol and applied to a Waters SepPak Plus Longbody SiO 2 cartridge. The cartridge was washed with dichlorimethane/methanol and then the product was eluted with dichloromethane/methanol/water (65:35:5) mL) and concentrated. The residue was dissolved in water and applied to a column of C18 silica (Waters Prep C18, 125 A, 5 The C18 silica was washed with water (50 mL) and then the product was eluted with 70% methanol (50 mL).

The resulting N-alkylated glycine, 0-alanine, and L-leucine compounds were characterized with 'H-nmr spectroscopy and MALDI-TOF mass spectroscopy.

Example N General Procedure for Deblocking of N-Alkylated L-Histidine and L-Tryptophan Compounds To the N-alkylated amino acid methyl ester from Example H (100 /mol) in dry methanol (7.3 mL) and dichloromethane (1.1 mL) under an argon atmosphere was added methanolic sodium methoxide (1 M, 50 ML). After 1-24 h, the mixture was neutralized with Amberlite IR-50S resin, filtered and concentrated. The residue was dissolved in 70% methanol and applied to a column of C18 silica (Waters Prep C18, 125 A, 5 g) and then the product was eluted with 70% methanol (50 mL). To the residue in water (3.7 mL) was added aqueous lithium hydroxide (1M, 0.3 mL).

After 0.5-2 h, the mixture was neutralized with Amberlite IR-50S resin, filtered and concentrated. The residue was dissolved in dichloromethane/methanol and WO 98/22487 PCT/CA97/00866 -48applied to a Waters SepPak Plus Longbody SiO, cartridge. The cartridge was washed with dichloromethane/methanol and then the product was eluted with dichloromethane/methanol/water (65:35:5) (20 mL) and concentrated. The residue was dissolved in water and applied to a column of C18 silica (Waters Prep C18, 125 A, 5 The C18 silica was washed with water (50 mL), and the product was eluted with 70% methanol (50 mL). The resulting N-alkylated L-histidine and L-tryptophan compounds were characterized with 'H-nmr spectroscopy and MALDI-TOF mass spectroscopy.

Example O General Procedure for Deblocking of N-Alkylated L-Arginine Compounds To the N-alkylated arginine methyl ester from Example H (100 /mol) in dry methanol (7.3 mL) and dichloromethane (1.1 mL) under an argon atmosphere was added methanolic sodium methoxide (1M, 50 After 1-24 h, the mixture was neutralized with Amberlite IR-50S resin, filtered and concentrated. The residue was dissolved in 70% methanol and applied to a column of C18 silica and then the product was eluted with 70% methanol (50 mL). To the residue in water (3.7 mL) wa then added aqueous lithium hydroxide (1M, 0.3 mL). After 0.5-2 h, the mixture was neutralized with Amberlite IR-50s (H resin, filtered and concentrated. The residue was dissolved in water and applied to column of C18 silica (Waters Prep C18, 125 A, 5 The C18 silica was washed with water (50 mL) and then the product was eluted with 50% methanol (50 mL). The resulting N-alkylated L-arginine compounds were characterized with 'H-nmr spectroscopy and MALDI-TOF mass spectroscopy.

Example P General Procedure for the Preparation of Mesylates To the alcohol from Example D (0.3 mmol) in dry tetrahydrofuran (2 mL) and dry pyridine (4 mL) under an argon atmosphere was added methanesulfonyl chloride mL). After 12-24 h, the mixture was washed with 0.5M HCI and extracted with WO 98/22487 PCT/CA97/00866 -49pentane. The pentane extracts were concentrated and the residue was purified on C18-silica to afford the mesylate derivative.

Example Q General Procedure for the Preparation of Azido Compounds To the mesylate from Example P (0.2 mmol) in dry DMF (8 mL) and dry THF (3 mL) under an argon atmosphere at 60"C was added sodium azide (5 mmol) and 18crown-6 (180 mg). After 2 hours, the reaction mixture was concentrated and the residue was purified on C18-silica. In some cases, the product was rechromatographed with silica gel using pentane/EtOAc as the eluant to afford the azido derivative.

Example R General Procedure for Reduction of Azido Groups to Primary Amines To a solution of the azido compound from Example S (15 4mol) in dry isopropanol (1 mL) and dry ethanol (1 mL) under an argon atmosphere, was added NaBH 4 (15 umol) and NiCl 2 (30 1mol). After 1 hour, the reaction mixture was neutralized with acetic acid (1 drop), concentrated and purified on C18-silica to afford the primary amine.

Example S General Procedure for Reductive Alkylation of Primary Amines To the primary amine from Example F or S (6.8 /mol) in dry methanol (1 mL) and dry dichloromethane (1 mL) under an argon atmosphere was added an aldehyde or ketone (3.4 mmol) and sodium triacetoxyborohydride (47 ,mol). After 24-48 hours, toluene (1 mL) was added and the mixture was concentrated and the residue purified on C18-silica gel.

WO 98/22487 PCT/CA97/00866 Example T General Procedure for Reductive Amination To the product from Example D (0.1 mmol) and a primary amine (0.45 mmol) in dry dichloromethane (2 mL), methanol (2 mL) and triethylorthoformate (1 mL) under argon, was added NaCNBH 3 (1 mmol). After 24 h, the mixture was concentrated and dissolved in toluene (1 mL) and purified on C18-silica gel (5 g).

Example U General Procedure for Deblocking of Secondary Amines To the O-lauroylated secondary amine from Example S or T (100 /mol) in dry methanol (7.1 mL) and dichloromethane (1.4 mL) under argon, was added methanolic sodium methoxide (1M, 50 After 1-24 h, the mixture was neutralized with Amberlite IR-50S resin, filtered and concentrated. The residue was dissolved in dichloromethane/methanol 2:1 and applied to a Waters SepPak Plus Longbody SiO cartridge. The cartridge was washed with dichloromethane/methanol and then -the product was eluted with dichloromethane/methanol/water (20 mL) and concentrated. The residue was dissolved in water and applied onto a column of C18 silica (Waters Prep C18, 125 A, 5 The C18 silica was washed with water mL) and then the product was eluted with methanol (50 mL). The resulting secondary amines were characterized with 1H-nmr spectroscopy and MALDI-TOF mass spectroscopy.

Example Al Synthesis of 2-Hydroxycyclopent-l-yl l-Thio-3-D-galactopyranoside The title compound was prepared according to procedures D, E and I above using 2-chlorocyclopentanone as the electrophile. Mass spectra data was as follows: M (calcd.): 280.34; M (found): 304.9 Selected nmr data was as follows: 'H-nmr (CD 3 OD): 6 4.44 4.42, 4.38, and 4.35.

WO 98/22487 WO 9822487PCT/CA97Ioo866 -51- Example A2 Synthesis of 2-Hydroxycyclohex-1.yi l-Thio-ftD.galactopyranoside The title compound was prepared according to procedures D, E and I above using 2-chiorocyciohexanone as the electrophile. Mass spectra data was as follows: M (calcd.): 294.34; M (found): 318.8 Selected nmr data was as follows: IH-nmr (CD 3 OD): 6 4.55 4.43, 4.39, and 4.34.

Example A3 Synthesis of 3-Hydroxy-l-phenylbut-1-yl l-Thio-ft-D-galactopyranoside The title compound was prepared according to procedures D, E and I above using 4-phenylbut-3-en-2-one as the electrophile. Mass spectra data was as -follows: M (calcd.): 345.43; M (found): 368.0 Selected nmr data was as follows: 1 H-nrnr (CD 3 OD): 6 4.45 4.43, 4.31, and 4.25.

Example A4 Synthesis of 3 -Hydroxynorborn-2-yl)methyl l-Thio-fl-D-galactopyranoside The title compound was prepared according to procedures D, E and I above using 3-methylene-2-norbomanone as the electrophile. Mass spectra data was as follows: M (calcd.): 320.41; M (found): 344.6 Selected nmr data was as follows: 'H-nmr (CD 3 O1D): 6 4.30 and 4.29.

Example Synthesis of 3-Hydroxycyclohept-1-ylIl-Thio-1-D-galactopyranoside The title compound was prepared according to procedures D, E and I above using cyclohept-en-l-one as the electrophile. Mass spectra data was as follows: M _(calcd.): 308.40; M (found): 332.1 Selected nmr data was as follows: 'H-nmr (CD 3 OD): 6 4.394 4.389, and 4.381.

WO 98/22487 WO 9822487PCT/CA97/00866 -52- Example Synthesis of 3-Hydroxycyclohept-1-ylIi-Thio-ot-D-galactopyranoside The title compound was prepared according to procedures D, E and I above using I-S-acetyl- 2 3 ,4,6-tetra--lauroy--thio-Dgactopyaose (from Example C' above) and cyclohept-en-l-one as the electrophile. Mass spectra data was as follows: M (calcd.): 308.40; M (found): 331.3 Selected nmr data was as follows: 'H-nmr (CD 3 OD): 6 5.44 J 5.8 Hz, H-i) and 5.45 J 5.8 Hz, H-i).

Example A6 Synthesis of 2 2 -Dimnethyl-4-hydroxycyclopent-1-yI l-Thio-f3-D-galactopyranoside The title compound was prepared according -to procedures D, E and I above using 4,4-dimethylcyclopent-2 -en-lI-one as the electrophile. Mass spectra data was as follows: M (calcd.): 308.40; M (found): 332.1 Selected nmr data was as follows: 'H-nmr (CD 3 OD): 6 4.34 4.3 15, 4.3 10, and 4.305.

Example A7 Synthesis of 3-Hydroxycyclopent-1-yI 1lbio-/#-D-galactopyranoside The title compound was prepared according to procedures D, E and I above using cyclopent-2-en-l-one as the electrophile. Mass spectra data was as follows: M (calcd.): 280.34; M (found): 304.9 Selected nmr data was as follows: IH-nmr (CD 3 OD): 6 4.36 4.355 and 4.34.

Example A8 Synthesis of 4-Hydroxypent-2-yI 1-Thio-fl-D-galactopyranoside The title compound was prepared according to procedures D, E and I above using pent-3-en-2-one as the electrophile. Mass spectra data was as follows: M (calcd.): 282.35; M (found): 305.3 Selected nmr data was as follows: 'H-nmr (CD 3 OD): 6 4.42 4.41, and 4.39.

WO 98/22487 WO 9822487PCT/CA97/00866 -53- Example A9 Synthesis of 2,2-Dimethyl-5-hydroxycyclobex-1-yI l-Thio-p-D-galactopyranoside The title compound was prepared according to procedures D, E and I above using 4,4-dimethylcyclohex-2-en-1I-one as the electrophile. Mass spectra data was as follows: M (calcd.) 322,42; M (found): 346.6 Selected nmr data was as follows: IH-nmr (CD 3 OD): 6 4.34 4.33, and 4.32.

Example AlO Synthesis of 3-Hydroxycyclohex-1-yI l-Thio-ft-D-galactopyranoside The title compound was prepared according to procedures D, E and I above using cyclohex-2-en-1-one as the electrophile. Mass spectra data was as follows: M -(calcd.): 294.37; M (found): 317.3 Selected nmr data was as follows: 1 H-nmr (CD 3 OD): 5 4.422 4.417, and 4.38.

Example All1 Synthesis of 4,4-Dimethyl-3-hydroxycyclohex-1-yl l-Thio-fl-D-galactopyranoside The title compound was prepared according to procedures D, E and I above using 6 ,6-dimethylcyclohex-2-en- 1-one as the electrophile.

Example B I Synthesis of 2-Aniinocyclopent-1-yl l-Thio-p-D-galactopyranoside The title compound was prepared according to procedures D, F and J above using 2 -chlorocyclopentanone as the electrophile. Mass spectra data was as follows: M (calcd.): 279.36; M (found): 276.3 Selected nmr data was as follows: IH-nmr (CD 3 OD): 6 4.46 4.45, 4.37 and 4.27.

WO 98/22487 PTCggo6 PCT/CA97/00866 -54- Example B2 Synthesis of 2-Am-inocyclohex-1-ylIl-Thio-j3-D-galactopyranoside The title compound was prepared according to procedures D, F and J above using 2-chiorocyclohexanone as the electrophile. Mass spectra data was as follows: M (calcd.): 293.38; M (found): 295.8 and 319.7 Selected nnir data was as follows: 'H-nmr (CD 3 OD): £3 4.48 4.44, 4.40 and 4.30.

Example B3 Synthesis of 3-Amino-1-phenylbut-1-yl l-Thio-1 3 -D-galactopyranoside The title compound was prepared according to procedures-DFand J above using 4-phenylbut-3-en-2-one as the electrophile. Mass spectra data was as follows: M (calcd.): 344.45; M (found): 345.1 Selected nmr data was as follows: 'H-nmr (CD 3 OD): £3 4.41 4.12, and 3.90.

Example B4 Synthesis of (3-Aminonorborn-2-yl)methyl l-Thio-I%-D-galactopyranoside The title compound was prepared according to procedures D, F and J above using 3 -methylene-2-norbornanone as the electrophile. Mass spectra data was as follows: M (calcd.): 319.42; M (found): 321.6 Selected- nmr data was as follows: 'H-nmr (CD 3 OD): £3 4.42 4.41, 4.38, and 4.35.

Example Synthesis of 3-Aminocyclohept-1-yI l-Thio-ft-D-galactopyranoside The title compound was prepared according to procedures D, F and J above using cyclohept-2-en-1-one as the electrophile. Mass spectra data was as follows: M (caled.): 307.41; M (found): 333.0 Selected nmr data was as follows: IH-nmr (CD 3 OD): 6 4.41 4.39, and 4.38.

WO 98/22487 WO 9822487PCT/CA97/00866 Example B6 Synthesis of 2,2-Dimethyl-4-aminocyclopent-1-yI l-Thio-ft-D-galactopyranoside The title compound was prepared according to procedures D, F and I above using 4,4-dimethylcyclopent-2-en-lI-one as the electrophile. Mass spectra data was as follows: M (calcd.): 307.41; M (found): 307.2 Selected nmr data was as follows: IH-nmr (CD 3 OD): 6 4.35 4.33, 4.32, and 4.30.

Example B6A Synthesis of 2j2-Dimnethyl-4-(mnethylamnino)cyclopent-1-yI 1-Thio-p-D-galactopyranoside The title compound was prepared according to procedures D, T and U above using 4,4-dimethylcyclopent-2-en-1I-one as the electrophile and methylamine as the primary amnine. Mass spectra data was as follows: M -(calcd.): 321.43; M (found): 322.7 Selected nmr data was as follows: 'H-nmr (CD 3 OD): 6 4.325 (H- 4.315, 4.308, 4.304.

Example B6B Synthesis of 2 ,2-Diinethyl-4(isopropylamino)cyclopent-1-yl 1-Thio-1-D-galactopyranoside The title compound was prepared according to procedures D, T and U above using 4,4-dimethylcyclopent-2-en- 1-one as the electrophile and isopropylamnine as the primary amine. Mass spectra data was as follows: M (calcd.): 349.48: M (found): 350.7 Selected nmr data was as follows: IH-nmr (CD 3 OD): 6 4/460 (H- 4.401, 4.400, 4.391.

WO 98/22487 WO 9822487PCT/CA97/00866 -56- Example B6C Synthesis of 2,2-DIxnethyl-4-(n-propylamnino)cyclopent-1-yl 1-Thio-fl-D-galactopyranoside The title compound was prepared according to procedures D, T and U above using 4,4-dimethyicyclopent-2-en-1I-one as the electrophile and n-propylamine as the primary amine. Mass spectra data was as follows: M (calcd.): 349.49; M (found): 350.5 Selected nmr data was as follows: 'H-nmr (CD 3 OD): 5 4.324 (H- 4.317, 4.310, 4.307.

Example B6D Synthesis of 2,2-Dimethyl-4-((R)-sec-butylamino)cyclopent-1-yl 1-Thio-g-D-galactopyranoside The title compound was prepared according to-procedures D, T and U above using 4,4-dimethylcyclopent-2-en- 1 -one as the electrophile and (R)-()-sec-butylamine as the primary amine. Mass spectra data was as follows: M (calcd.): 364.52; M (found): 364.6 Selected nmr data was as follows: 'H-nmr (CD 3 OD): 4.328 4.319, 4.313, 4.311.

Example B6E Synthesis of 2 ,2-Dimethyl-4- ((S)-sec-butylamino)- -cyclopent-1-yl 1-Thio-1-D-galactopyranoside The title compound was prepared according to procedures D, T and U above using 4 ,4-dimethylcyclopent-2-en- 1 -one as the electrophile and +)-sec-butylamfine as the primary amine. Mass spectra data was as follows: M (calcd.): 364.52; M (found): 364.6 Selected nmr data was as follows: IH-nmr (CD 3 OD): 4.333 4.330, 4.300, 4.290.

WO 98/22487 WO 9822487PCT/CA97/00866 -57- Example B6F Synthesis of- 2 ,2-Dimethyl-4-(pent-3-ylamino)cyclopent-1-ylIl-Thio-ft-D-galactopyranoside The title compound was prepared according to procedures T and _U above using 4,4-dimethylcyclopent-2-en- I-one as the electrophile and 3-pentylamine as the primary amine. Mass spectra data was as follows: M (calcd.): 377.53; M (found): 376.7 Selected nmr data was as follows: IH-nmr (CD 3 OD): 5 4.333 (H- 4.329, 4.300, 4.290.

Example B6G Synthesis of 2,2-DhmethyI-4-(n-hexylamnino)- -cyclopent-1-yI l-Thio-j3-D-galactopyranoside The title compound was prepared according to procedures D, T and U above using 4,4-dimethylcyclopent-2-en-1-one as the electrophile and n-hexylamine a the primary amine. Mass spectra data was as follows: M (calcd.): 391.57; M (found): 39 4.3 Selected nmr data was as follows: '1{-nmr (CD 3 OD): 6 4.336 (H- 4.332, 4.303, 4.291.

Example B6H Synthesis of 2,2-Dimnethyl-4-(cyclobut-1-ylamino).

cyclopent-1-yI 1-Thio-fl-D-galactopyranoside The title compound was prepared according to procedures D, T and U above using 4 ,4-dimet hylcyclopent-2-en- 1-one as the electrophile and cyclobutyl amine as the primary amine. Mass spectra data was as follows: M (calcd.): 3617.50; -M (found): 361.6 Selected nmr data was as follows: IH-nmr (CD 3 OD): 4.315 4.300, 4.292, 4.290.

WO 98/22487 WO 9822487PCT/CA97/00866 -58- Example B61 Synthesis of 2,2-Dimnethyl-4-(3 ,3-dimnethylcyclobut-1-ylamino)- .cyclopent-1-yI l-Thio-13-D-galactopyranoside The title compound was prepared according to procedures D, T and U above using 4 ,4-dimethylcyclopent-2-en- 1 -one as the electrophile and 3 ,3-dimethylcyclobut- 1-ylamine as the primary amine. Mass spectra data was as follows: M (calcd.): 389.55; M (found): 392.2 (M+H 4 Selected nmr data was as follows: IH-nmr

(CD

3 OD): 6 4.324 4.3 11, 4.305, 4.294.

Example B6J Synthesis of 2 ,2-Dimethyl-4- (cyclopent-1-ylamino)cyclopent-1-yI 1-Thio-q-D-galactopyranoside The title compound was prepared according to procedures D, T and U above using 4,4-dimethylcyclopent-2-en-1I-one as the electrophile and cyclopentylamine as the primary amine. Mass spectra data was as follows: M (calcd.): 375.52; M (found): 376.6 Selected nmr data was as follows: 'H-nmr (CD 3 OD): 4.322 4.310, 4.304, 4.295.

Example B6K Synthesis of 2,2-Dimethyl-4- (cyclohex-1-ylamino)cyclopent-1-yI 1-Thio-1-D-galactopyranoside- The title compound was prepared according to procedures D, T and U above using 4,4-dimethylcyclopent-2-en- 1-one as the electrophile and cyclohexylamine as the primary amine. Mass spectra data was as follows: M (calcd.): 389.55; M (found): 391.2 Selected nmr data was as follows: 'H-nmr (CD 3 OD): 6 4.3 19 (H- 4.3 10, 4.307, 4.293.

WO 98/22487 WO 9822487PCT/CA97/00866 -59- Example B6L Synthesis of 2,2-Diinethyl-4-(4-methylcyclohex-.1yaino).

cyclopent-1-yI l-Thio-fl-D-galactopyranoside The title compound was prepared according to procedures D, T and U above using 4,4-dimethylcyclopent-2-en-1I-one as the electrophile and 4-methylcyclohex-1ylamine as the primary amine. Mass spectra data was as follows: M (calcd.): 403.47; M (found): 404.8 Selected nmr data was as follows: IH-nmr (CD 3 OD): 4.333 4.312, 4.300, 4.295.

Example B6Q Synthesis of 2 2 -DimethyI-4-(3-methylcyclopent-1-ylamino).

cyclopent-1-ylI -Thio-fl-D-galactopyranoside The title compound was prepared according to procedures D, T and U above using 4 ,4-dimethylcyclopent-2-en- 1 -one as the electrophile and 3-methylcyclopent- 1ylamine as the primary amine. Mass spectra data was as follows: M (calcd.): 389.55; M (found): 390.7 Selected nmr data was as follows: 'H-nmr (CD 3 OD): 4.383 4.325, 4.300, 4.292.

Example B6R Synthesis of 2 ,2-Dimnethyl-4- (3 ,3-dimethylcyclopent-1-ylamino)cyclopent-1-ylI1-Thio-fl-D-galactopyranoside The title compound was prepared- according to procedures D, T and U above using 4,4-dimethylcyclopent-2-en-1-one as the electrophile and 3,3dimethylcyclopent-1-ylamine as the primary amine. Mass spectra data was as follows: M (calcd.): 4.295; M (found): 404.3 Selected nmr data was as follows: 'H-nmr (CD 3 OD): .5 4.322 4.305, 4.300, 4.295.

WO 98/22487 WO 9822487PCT/CA97/00866 Example B6T Synthesis of 2 2 -Dimethyl-4-(3-methylcyclohex-1-ylamino).

cyclopent-1-yiIl-Thio-ft-D-galactiopyranoside The title compound was prepared according to procedures D, T and U above using 4,4-dimethylcyclopent-2-en-1I-one as the electrophile and 3-methylcyclohex-1ylamine as the primary amine. Mass spectra data was as follows: M (calcd.): 403.57; M (found): 404.8 Selected nmr data was as follows: 'H-nmr (CD 3 OD): 4.326 4.313, 4.303, 4.294.

Example B7 Synthesis of 3-Aminocyclopent-1-yl 1-Thio-ft-D-galactopyranoside, The title compound was prepared according to procedures D, F and J above using cyclopent-2-en-l-one as the electrophile. Mass spectra data was as follows: M (calcd.): 279.35; M (found): n.a. Selected nmr data was as follows: lH-nmr

(CD

3 OD): 5 4.46, 4.40, 4.38, and 4.34 (4 d, J 10 Hz), 3.88 (br 2.61, 2.27, 2.15, 1.82, and 1.64 (5 in).

Example B8 Synthesis of 4-Aminopent-2-yI 1-Thio-fl-D-galactopyranoside The title compound was prepared according to procedures D, F and J above using pent-3-en-2-one as the electrophile. Mass spectra data was as follows: M (calcd.): 281.37; M (found): 283.4 Selected nmr data was as follows: IHnrnr (CD 3 OD): 5 4.41 4.40, and 4.36.

Example Synthesis of 3-Aminocyclohex-1-yI l-Thio-fl-D-galactopyranoside The title compound was prepared according to procedure D, F and J above using cyclohex-2-en-1-one as the electrophile. Mass spectra..data was as follows: M WO 98/22487 PCT/CA97/00866 -61- (calcd.): 293.38; M (found): 317.9 Selected nmr data was asjollows: 'H-nmr (CD 3 OD): 5 4.54 4.52, 4.49, and 4.47.

Example Bl11 Synthesis of 3 -Amino-4,4-dimethylcyclohex-1-yI l-Thio-fl-D-galactopyranoside The title compound was prepared according to procedure D, F and J above .using 6 6 -dimethylcyclohex-2-en- 1-one as the electrophile.

Example C I Synthesis of 2-Acetamidocyclopent-1-yl l-Thio-fl-D-galactopyratioside The title compound. was prepared according to procedures D, F, J and K above using 2-chlorocyclopent-l1-one as the electrophile. Mass spectra data was as follows: M (calcd.): 321.39; M (found): 345.8 Selected nmr data was as follows: 'H-nmr (CD 3 OD): 6 4.53 4.44, 4.32, and 4.24.

Example C2 Synthesis of 2-Acetamidocyclohex-1-yI l-Thio-fl-D-galactopyranoside The title compound was prepared according to procedures D, F, J and K above using 2 -chlorocyclohexan-1I-one as the electrophile. Mass spectra data was as follows: M (calcd.): 335.42; M (found): 359.4 Selected nmr data was as follows: IH-nmr (CD 3 OD): 5 4.43 4.42, 4.32, and 4.29.

Example C3 Synthesis of 3-Acetamido-1-phenylbut-1-yiIi-Thio-fl-D-galactopyranoside The title compound was prepared according to procedures D, F, J and K above using 4-phenylbut-3-en-2-one as the electrophile. Mass spectra -data was as follows: M (calcd.): 386,48; M (found): 408.3 Selected nmr data was as follows: IH-nmr (CD 3 OD): 6 4.32 4.25, 3.83, and 3.79.

WO 98/22487 WO 9822487PCT/CA97/00866 -62- Example Synthesis of 3-Acetamidocyclohept-1-ylIl-Thio-13-D-galactopyranoside The title compound was prepared according to procedures D, F, J and K above using cyclohept-2-en-1-one as the electrophile. Mass spectra data was as follows: M (calcd.): 349.42; M (found): 372.5 Selected nmr data was as follows: 'H-nmr (CD 3 OD): 5 4.403 4.397, 4.34, and 4.33.

Example C7 Synthesis of 3-Acetamidocyclopent-1-ylI1-Thio-fl-D-galactopyranoside The title compound was prepared according to procedures D, F, J and K above using cyclopent-2-en-1-one as the electrophile. Mass spectra data was as follows: M (calcd.): 321.39; M (found): 349.5 Example C8 Synthesis of 4-Acetamidopent-2-yI 1-Thio-1-D-galactopyranoside The title compound was prepared according to procedures D, F, J and K above using pent-3-en-2-one as the electrophile. Mass spectra data was as follows: M (calcd.): 323.40; M (found): 347.7 Selected nmr data was as follows: IH-nmr (CD 3 OD): 6 4.42 4.38, 4.37, and 4.35.

Example Synthesis of 3-Acetamidocyclohexyl 1-Thio-fl-D-galactopyranoside The title compound was prepared according to procedures D, F, J and K above using cyclohex-2-en-1 -one as the electrophile. Mass spectra data was as follows: M (calcd.): 335.42; M (found): 373.7 Selected nmr data was as follows: 'H-nmr (CD 3 OD): 6 4.52 4.464, and 4.455.

WO 98/22487 WO 9822487PCT/CA97/00866 -63- Example Cli1 Synthesis of 3-Acetido4,4-dinethylcyclohexyl l-Thio-fl-D-galactopyranoside The title compound was prepared according to procedures D, F, J and K above 'using 6,6-dimethylcyclohex-2-en-1I-one as the electrophile.

Example D I Synthesis of 2-(2-Carboxybenzamido)cyclopent-1-yI l-Thio-fl-D-galactopyranoside The title compound was prepared according to procedures D, F, G and L above using 2-chlorocyciopentan-i1-one as the -electrophile. Mass spectra data was as follows: M (calcd.): 427.47; M (found): 450.5 Selected nmr data was as follows: 'H-nmr (CD 3 OD): 6 4.69 4.58, 4.27, and 4.22._ Example D2 Synthesis of 2-(2-Carboxybenzamido) cyclohex-1-yl l-Thio-fc-D-gaactopyranoside, The title compound was prepared according to procedures D, F, G and L above using 2-chlorocyclohexan- I-one as the electrophile. Mass spectra data was as follows: M (calcd.): 441.50; M (found): 465.9 Selected nmr data was as follows: 'H-nmr (CD 3 OD): 5 4.54 4.52, 4.50, and 4.35.

Example D3 Synthesis of 3-(2-Carboxybenzaxnido)-1-phenylbut-1-yI l-Thio-ft-D-galactopyranoside The title compound was prepared according to procedures D, F, G and L above using 4-phenylbut-3-en-2-one as the electrophile. Mass spectra data was as follows: M (calcd.): 492.56; M (found): 513.0 Selected nmr data was as follows: 'H-nmr (CD 3 OD): 5 4.41 4.115, 4. 110, and 3.90..- WO 98/22487 PCT/CA97/00866 -64- Example D4 Synthesis of [3-(Carboxybenzainido) norborn-2-yI]methyI l-Thio-I3-D-galactopyranoside The title compound was prepared according to procedures D, F, G and L above using 3 -methylene-2-norbomanone as the electrophile. Mass spectra data was as follows: M (calcd.): 467.54; M (found): 492.9 Selected nmr data was as follows: 'H-nmr (CD 3 OD): 6 4.39 4.34, 4.31, and 4.26.

Example Synthesis of 3 -(2-Carboxybenzamido)cyclohept.1yI l-Thio-I#-Dmgalactopyranoside The title compound was prepared according to procedures D, F, G and L above using cyclohept-2-en-l-one as the electrophile. Mass spectra data was as follows: M (calcd.): 453.52; M (found): 479.6 Selected nmr data was as follows: kH-nmr (CD 3 OD): 5 4.53 4.51, 4.42, and 4.40.

Example D8 Synthesis of 3 -(2-Carboxybenzamnido) pent-2-yI l-Thio-ft-D-galactopyranoside The title compound was prepared according to procedures D, F, G and L above using pent-3-en-2-one as the electrophile. Mass spectra data was as follows: M (calcd.): 429.48; M (found): 452.7 Selected nmr data was as follows: 'H-nmr (CD 3 OD): 6 4.42 4.41, 4.40, and 4.35.

Example D9 Synthesis of- 5-(2-Carboxybenzarnido)-2 ,2-dixnethylcyclohex-1-yI l-Thio-13-D-galactopyranoside The title compound was prepared according to procedures D, F, G and L above using 4 4 -dimethylcyclohex-2-en- I-one as the electrophile. Mass spectra data was as follows: M (calcd.): 469.55, M (found): 492.4 WO 98/22487 PCT/CA97/00866 Example Synthesis of 3 -(2-Carboxybenzamido)cylohex-1-yl 1-Thio--D-galactopyranoside The title compound was prepared according to procedures D, F, G and L above using cyclohex-2-en-1-one as the electrophile. Mass spectra data was as follows: M (caled.): 441.50, M (found): n.a. Selected nmr data was as follows: IH-nmr

(CD

3 OD): 5 4.37 4.34, and 4.32.

Example El Synthesis of No- [2-(1-Thio-p-D-galactopyranosyl)cyclopent-1-yl]glycine The title compound-was prepared according to procedures D, H and M above using 2-chlorocyclopentan-1-one as the electrophile and glycine tert-butyl ester as the amino acid ester. Mass spectra data was as follows: M (calcd.): 337.39; M (found): 359.8 Selected nmr data was as follows: 'H-nmr (CD 3 OD): 6 4.44 4.41, 4.40, and 4.34.

Example E2 Synthesis of No- [2-(1-Thio-P-D-galactopyranosyl)cyclohex-1-yl]glycine The title compound was prepared according to procedures D, H and M above using 2-chlorocyclohexan-l-one as the electrophile and glycine terr-butyl ester as the amino acid ester. Mass spectra data was as follows: M (calcd.): 351.42; M (found): 353.5 376.5 Selected nmr data was as follows: 'H-nmr 5 4.48 4.47, 4.36, and 4.29.

Example E3 Synthesis of Na-[4-Phenyl-4-( -thio--D-galactopyranosyl)but-2-yl]glycine The title compound was prepared according to procedures D, H and M above using 4-phenylbut-3-en-2-one as the electrophile and glycine tert-butyl ester as the WO 98/22487 PCT1C-A97/00866 -66amino acid ester. Mass spectra data was as follows: M (calcd.): 401.48; M (foiund): 403A1 Selected nmr data was as follows: 'H-nmr (CD 3 OD): 6 4.29 4.18, 3.92, and 3.9 1.

Example E4 Synthesis of No-[ 3 -((l-Thio-1-D-galactopyranosyl)metbyl)norborn.2yI]glycine The title compound was prepared according to procedures D, H and M above using 3-methylene-2-norbomanone as the electrophile and glycine -tert-butyl ester as the amino acid ester. Mass spectra data was as follows: M (calcd.): 377.46; M (found): 401.4 Selected nmr data was as follows: 'H-nmr (CD 3 OD): 4.42 4.40, 4.383, 4.377, and 4.35.

Example Synthesis of Na[-ITi---aatprnslccoet1yllcn The title compound was prepared according to procedures D, H and M above using cyclohept-2-en-l-one as the electrophile and glycine ten-butyl ester as the amino acid ester. -Mass spectra data was as follows: M (calcd.): 365.45; M (found): 367.4 389.9 Selected nmr data was as follows: 'H-nmr (CD 3 OD): 4.46 4.45, 4.42, and 4.38.

Example ES' Synthesis of Not- [3-(l-Thio-'-D-galactopyranosy) cyclohept-1-yllglycine The title compound was prepared according to procedures D, H and M above using 1 -S-acetyl-2 ,3 6-tetra-O-lauroyl-l1-thio-oa-D-galactopyranose (from Example C' above), cyclohept-2-en-1-one as the electrophile and glycine tert-butyl ester as the amino acid ester. Mass spectra data was as follows: M (calcd.): 365.45; M (found): 366.6 Selected nmr data was as follows: 'H-nmr (CD 3 OD): 5 5.51 J Hz, H-i (major), 5.46 J 5.5 Hz, 5.47 J 5.5 Hz, H-i (minor)), 5.48 J 5.5 Hz, H-i1).

WO 98/22487 WO 9822487PCT/CA97/00866 -67- Example E6 Synthesis of Noe-[4,4..DimethyI-3-(1-thio-p3-D-galactopyranosy).

cyclopent-1-yllglycine The title compound was prepared according to procedures D, H and M above using 4,4-dimethylcyplopent-2-en-1I-one as the electrophile and glycine tert-butyl ester as the amino acid ester. Mass spectra data was as follows: M (calcd.): 365.44; M (found): 368.,0 Selected nmr data was as follows: 'H-nmr (CD 3 OD): 4.330 4.325, 4.320, and 4.30.

Example E7 Synthesis of Na[-ITi---aatprnslccoet1y~lcn The title compound was prepared according to procedure s D, H and M above using cyclopent-2-en-1-one as the electrophile and glycine tert-butyl ester as the amino acid ester. Mass spectra data was as follows: M (calcd.): 337.39; M (found): 360.9 Selected nmr data was as follows: IH-nmr (CD 3 OD): 6 4.38 4.375, 4.36, and 4.35.

Example E8 Synthesis of Noe-[ 4 -(l-Thio-( 3 -D-galactopyranosyI)pent-2-yl]glycine The title compound was prepared according to procedures D, H and M above using pent-3-en-2-one as the electrophile and glycine tert-butyl ester as the amino acid ester. Mass spectra data was as follows: M (calcd.): 338.39; M (found): 363.9 Selected nmr data was as follows: 'H-nmr (CD 3 OD): 15 4.43, 4.42, 4.37 and 4.36.

WO 98/22487 WO 9822487PCTICA97/00866 -68- Example E9 Synthesis of Not-[ 4 ,4-Dixnethyl-3-(l-thio-q3-D..galactopyranosyl).

cyclohex-1-yIlglycine The title compound was prepared according to procedures D, H and M above using 4,4-dimethyicyclohex-2-en-1-one as the electrophile and glycine terr-butyl ester as the amino acid ester. Mass spectra data was as follows: M (calcd.): 379.47; M (found): 380.6 (M+H 4 403.5 Selected nmr data was as follows: IHnmr (CD 3 OD): 6 4.38 4.36, 4.34, and 4.31.

Example ElO Synthesis of The title compound was prepared according to procedures D, H and M above using cyclohex-2-en-1-one as the electrophile and glycine ter-butyl ester as the amino acid ester. Mass spectra data was as follows: M (calcd.): 351.42; M (found): 377.1 Selected nmr data was as follows: 'H-nmr (CD 3 OD): 3 4.46 4.44, 4.40, and 436.

Example Eli1 Synthesis of 2,2-dimethylcyclohex-1-yI]glycjne The title compound was prepared according to procedures D, H and M above using 6 ,6-dimethylcyclohex-2-en- I-one as the electrophile and glycine tert-butyl ester as-ithe amino acid ester.

Example F 1 Synthesis of N#[-lTi---aatprnsy~ylpn--l-f-lnn The title compound was prepared according to procedures D, H and M above using 2 -chlorocyclopentan-1I-one as the electrophile and i3-alanine tert-butyl ester as the amino acid ester. Mass spectra data was as follows: M (calcd.): 351.42; M WO 98/22487 WO 9822487PCT/CA97/00866 -69- (found): 372.9 (M+Nal). Selected nmr data was as follows: IH-nmr (CD 3 OD): 4.54 4.52, 4.36, and 4.35.

Example F2 Synthesis of Ni3-[2-(1-Thio-I3-D-galactopyranosy)cyclohex-1-yI]-fj-alanine The title compound was prepared according to procedures D, H and M above using 2-chlorocyclohexan- I-one as the electrophile and f3-alanine tert-butyl ester as the amino acid ester. Mass spectra data was as follows: M (calcd.): 365.45; M (found): 367.4 389.9 412.0 Selected nmr data was as follows: IH-nmr (CD 3 OD): 6 4.47 4.42, 4.41, and 4.33.

Example F3 Synthesis of NI3-[4-Pheny1-4-(1-thio-fl-D-galactopyranosy)but-2-yi]-(3-alanine The title'compound was prepared according to procedures D, H and'M above using 4-phenylbut-3-en-2-one as 'the electrophile and 0-alanine tert-butyl ester as the amino acid ester. Mass spectra data was as follows: M (calcd.): 415.50; M (found): 417.0 Selected nmr data was as follows: IH-nmr (CD 3 OD): 6 4.28 4.17, 3.97, and 3.96.

Example F4 Synthesis of Nj3-[3-((1-Thio-O-D-galactopyranosyI)methyI) norborn-2-yI]-jp-ala i e The title compound was prepared according to procedures D, H and M above using 3-methylene-2-norbomanone as the electrophile and /3-alanine tert-butyl ester as the amino acid ester. Mass spectra data was as follows: M (calcd.): 391.48; M (found): 393.6 Selected nmr data was as follows: 'H-nmr (CD 3 OD): 4.40 4.37, 4.34, and 4.33.

WO 98/22487 PCT/CA97/00866 Example Synthesis of NP-[3-(I-Thio--D-galactopyranosyl)cyclohept-1-yl]--alanine The title compound was prepared according to procedures D, H and M above using cyclohept-2-en-1-one as the electrophile and 0-alanine tenrt-butyl ester as the amino acid ester. Mass spectra data was as follows: M (calcd.): 379.45; M (found): 381.7 403.5 426.0 Selected nmr data was as follows:- 'H-nmr (CD30D): 6 4.46 and 4.38.

Example F6 Synthesis of N#-[4,4-Dimethyl-3-(1-thio-P-D-galactopyranosyl)cyclopent-l-yl]-f-alanine The title compound was prepared according to procedures D, H and M above using 4 4 -dimethylcyclopent-2-en- I-one as the electrophile and f-alanine tert-butyl ester as the amino acid ester. Mass spectra data was as follows: M (calcd.): 379.44; M (found): 383.2 Selected nmr data was as follows: !H-nmr (CD 3 OD): 4.34 4.33, 4.315, and 4.310.

Example F7 Synthesis of N#-[3-(1-Thio--D-galactopyranosyl)cyclopent-1-yl]-8-alanine The title compound was prepared according to procedures D, H and M above using cyclopent-2-en-1-one as the electrophile and O-alanine tert-butyl ester as the amino acid ester. Mass spectra data was as follows: M (calcd.): 351.42; M (found): 375.1 Selected nmr data was as follows: IH-nmr (CD30D): 6 4.41 (Hand 4.40.

WO 98/22487 WO 9822487PCT/CA97/00866 -71- Example F8 Synthesis of Nj 3 4 -(1-Thio-fl-D-galactopyranosyl) pent-2-yII-f3-alanine The title compound was prepared according to procedures D, H and M above using pent-3-en-2-one as the electrophile and 0-alanine ten-butyl ester as the amino acid ester. Mass spectra data was as follows: M (calcd.): 352.42; M (found): 356.0 Selected nmr data was as follows: IH-nmr (CD 3 OD): 6 4.49 4.440, and 4.435.

Example F9 Synthesis of cyclohex-1-yI]-13-alanine The title compound was prepared according to procedures D, H and M above _using 4,4-dimethylcyclohex-2-en-lI-one as the electrophile and 6l-alanine tert-butyl ester as the amino acid ester. Mass spectra data was as follows: M (calcd.): 393.50; -M (found): 399.3 (M+H 4 419.5 442.4 Selected nmr data was as follows: 'H-nmr (CD 3 OD): 8 4.35 4.34, and 4.32.

Example FlO Synthesis of N#[-lTi-tDglcoyaoy~ylhx1y]f-lnn The title compound was prepared according to procedures D, H and M above using cyclohex-2 -en-l-one as the electrophile and 0-alanine tert-butyl ester as the amino acid ester. Mass spectra data was as follows: M (caled.): 365.45; M (found): 367.0 389.9 Selected nmr data was as follows: I'H-nmr

(CD

3 OD): 6 4.46 4.44, 4.43, and 4.36.

WO 98/22487 WO 9822487PCT/CA97/00866 -72- Example F I1I Synthesis of 2,2-dimethylcyclohex-1-yIJ-j3-alanine The title compound was prepared according to procedures D, H and M above using 6,6-dimethyfcyclohex-2-en-lI-one as the electrophile and fl-alanine tert-butyl ester as the amino acid ester.

Example G 1 Synthesis of Na-[ 2 -(l-Thio-I 3 -D-galactopyranosyI)cyclopent-1-y]4,.Ieucine The title compound was prepared according to procedures D, H and M above using 2-chlorocyclopentan-1I-one as the electrophile and L-leucine tert-butyl ester as the amino acid ester. Mass spectra data was as follows: M (calcd.): 393.50; M (found): 396.4 Selected nmr data was as follows: 'H-nmr (CD 3 OD): 4.47 4.43, 4.36, and 4.34.

Example G2 Synthesis of No-[-(-hof--aatpraoy)ccoe--i--ecn The title compound was prepared according to procedures D, H and M above using 2-chlorocyclohexan- I-one as the electrophile and L-leucine tert-butyl ester as the amino acid ester. Mass spectra data was as follows: M (calcd.): 407.53; M (found): 410.9, 435.5 Selected nmr data was as follows: IH-nmr.

(CD

3 OD): B 4.49 4.44, 4.41, and 4.37.

Example G3 Synthesis of Nei- 14-Phenyl-4- (1-thio-fl-D-galactopyranosy) but-2-yI~.Ieucine The title compound was prepared according to procedures D, H and M above using 4-phenylbut-3-en-2-one as the electrophile and L-leucine tert-butyl ester as the amino acid ester. Mass spectra data was as follows: M (calcd-.): 458.59; M (found): WO 98/22487 PCT/CA97/00866 -73- 480.5 Selected nmr data was as follows: 'H-nmr (CD 3 OD): 5 4.39 4.-36, 4.29, and 4.21.

Example Synthesis of Nix-[1- (1-Thio-ft-D-galact opyranosyl) cyclohept-3-yl]-leucine The title compound was prepared according to procedures D, H and M above using cyclohept-2-en-1-one as the electrophile and L-leucine tert-butyl ester as the amino acid ester. Mass spectra data was as follows: M (calcd.):-421.55; M (found): 421.7 448.0 Selected nmr data was as follows: 1 H-nmr

(CD

3 OD): 6 4.44 4.43, and 4.36.

Example G6 Synthesis of Na-[4,4-Dimethyl-3-(1-thio-B-D-galactopyranosyl)cyclopent-1-yl]-L-leucine The title compound was prepared according to procedures D, H and M above using 4 4 -dimethylcyclopent-2-en-1-one as the electrophile and L-leucine tert-butyl ester as the amino acid ester. Mass spectra data was as follows: M (caled.): 421.55; M (found): 422.3 Selected nmr data was as follows: 'H-nmr (CD30D): 4.320 and 4.315.

Example G7 Synthesis of Na-[3-(l-Thio--D-galactopyranosyl)cyclopent-1-y]-L-leucine The title compound was prepared according to procedures D, H and M above using cyclopent-2-en-1-one as the electrophile and L-leucine tert-butyl ester as the amino acid ester. Mass spectra data was as follows: M (calcd.): 393.50; M (found): 393.6 417.0 Selected nmr data was as follows: 'H-nmr

(CD

3 OD): 5 4.380 4.375, 4.370 and 4.367.

WO 98/22487 PCT/CA97/00866 -74- Example G8 Synthesis of Na-[4- (l-Thio--D-galactopyranosyl)pent-2-yl]-L-leucine The title compound was prepared according to procedures D, H and M above using pent-3-en-2-one as the electrophile and L-leucine tert-butyl ester as the amino acid ester. Mass spectra data was as follows: M (calcd.): 395.51; M (found): 396.8 419.1 and 440.9 Selected nmr data was as follows: 'H-nmr (CD 3 OD): 3 4.42 4.41, 4.405 and 4.40.

Example G9 Synthesis of Na-[4,4-Dimethyl-3-(1-thio-3-D-galactopyranosyl)cyclohex-1-yl]-L-eucine The title compound was prepared according to procedures D, H and M above using 4,4-dimethylcyclohex-2-en-1 I-one as the electrophile and L-leucine tert-butyl ester as the amino acid ester. Mass spectra data was as follows: M (calcd.): 436.58; M (found): 438.0 461.4 Selected nmr data was as follows: 'Hnmr (CD 3 OD): 5 4.38 and 4.34.

Example Synthesis of No- [3-(1-Thio-o-D-galactopyranosyl)cyclohex-1-yl]-Lleucine The title compound was prepared according to procedures D, H and M above using cyclohex-2-en-1-one as the electrophile and L-leucine terr-butyl ester as the amino acid ester. Mass spectra data was as follows: M (calcd.): 407.53; M (found): 408.4 Selected nmr data was as follows: 'H-nmr (CD 3 OD): 6 4.46 4.42, 4.40, and 4.33.

WO 98/22487 PCT/CA97/00866 Example H1 Synthesis of N-[2-(l-Thio-/-D-galactopyranosyl)cyclopent-l-yl]-L-histidine The title compound was prepared according to procedures D, H and N above using 2-chlorocyclopentan-1-one as the electrophile and L-histidine methyl ester as the amino acid ester. Mass spectra data was as follows: M (calcd.): 417.48; M (found): 418.7 Selected nmr data was as follows: 'H-nmr (CD 3 OD): 6 4.45 4.41, 4.40, and 4.29.

Example H2 Synthesis of Na-[2-(l-Thio-O-D-galactopyranosyl) cyclohex-l-yl]-L-histidine The title compound was prepared according to procedures D, H and N above using 2-chlorocyclohexan-l-one as the electrophile and L-histidine methyl ester as the amino acid ester. Mass spectra data was as follows: M (calcd.): 431.50; M (found): 433.6 Selected nmr data was as follows: 'H-nmr (CD 3 OD): 6 4.52 4.45, 4.40, and 4.28.

Example H3 Synthesis of No-[4-Phenyl-4-(1-thio-/-D-galactopyranosyl)but-2-yl]-L-histidine The title compound was prepared according to procedures D, H and N above using 4-phenylbut-3-en-2-one as the electrophile and L-histidine methyl ester as the amino acid ester. Mass spectra data was as follows: M (caled.): 481.56; M (found): 482.8 Selected nmr data was as follows: 'H-nmr (CDOD): 5 4.38 4.36, 4.23, and 4.16.

Example Synthesis of No-[ 3 -(1-Thio- 3 -D-galactopyranosyl)cyclohept- -yl]-L-histidine The title compound was prepared according to procedures D, H and N above using cyclohept-2-en-1-one as the electrophile and L-histidine methyl ester as the WO 98/22487 WO 9822487PCT/CA97/00866 -76amino acid ester. Mass spectra data was as follows: M (calcd.): 445.54; M (found): 448.0 Selected nmr data was as follows: 'H-nmr (CD 3 OD): -3 4.50 (H-i1), 4.44, 4.41, and 4.32.

Example H6 Synthesis of Na-[4,4-Dimethyl-3-(1-thio-3-D-galactopyranosyl)cyclopent-1-yl]-L-histidine The title compound was prepared according to procedures D, H and N above using, 4,4-dimethylcyclopent-2-en- I-one as the electrophile and L-histidine methyl ester as the amino acid ester. *Mass spectra data was as follows: M (calcd.): 445.54; M (found): 447.6 Selected nmr data was as follows: IH-nmr (CD 3 OD): a 4.33 4.32, 4.305, and 4.30.

Example- H7 Synthesis of Nci-[3- (l-Thio-I3-D-galactopyranosyI)cyclopent-1-yI]-L-histidine The title compound was prepared according to procedures D, H and N above using cyclopent-2-en-1-one as the electrophile and L-histidine methyl ester as the amino acid ester. Mass spectra data was as follows: M (calcd.): 418.48; M (found): 418.0 Selected nmr data was as follows: 'H-nmr (CD 3 OD): 5 4.39 4.38, 4.36, and 4.32.

Example H8 Synthesis of Not- (I-Tio-fl-D-galactopyranosyl) pent-2-yl]-LIhistidine The title compound was prepared according to procedures D,-H and N above using pent-3-en-2-one as the electrophile and L-histidine methyl ester as the amino acid ester. Mass spectra data was as follows: M (calcd.): 419.49; M (found): 420.2 30 Selected nmr data was as follows: 'H-nmr (CD 3 OD): 5 4.44 4.41, 4.40, and 4.36.

SWO 98/22487 PCTICA97/00866 -77- Example H9 Synthesis of Noa-[4,4-Dimethyl-3-(1-thio--D-galactopyranosyl)cyclohex-1-yl]-L.-histidine The title compound was prepared according to procedures D, H and N above using 4 4 -dimethylcyclohex-2-en-l-one as the electrophile and L-histidine ethyl ester as the amino acid ester. Mass spectra data was as follows: M (calcd.): 459.56; M (found): 462.2 Selected nmr data was as follows: 'H-nmr (CD 3 OD): 4.364 4.357, and 4.34.

Example Synthesis of Na-[3-(1-Thio-O-D-galactopyranosyl)cyclohex-1-yl]-Lhistidine The title compound was prepared according to procedures D, H and N above using cyclohex-2-en-l-one as the electrophile and L-histidine methyl ester as the amino acid ester. Mass spectra data was as follows: M (calcd.): 431.51; M (found): 433.2 Selected nmr data was as follows: 'H-nmr (CD 3 OD): 5 4.43 4.425, 4.39 and 4.35.

Example 11 Synthesis of N-[2-(-Thio--D-galactopyranosyl)cyclopent-1-yl]--tryptophan The title compound was prepared according to procedures D, H and N above using 2-chlorocyclopentan- I-one as the electrophile and L-tryptophan methyl ester as the amino acid ester. Mass spectra data was as follows: M (calcd.): 466.55; M (found): 467.5 Selected nmr data was as follows: 'H-nmr (CD 3 OD): 6 4.51 4.39, 4.28 and 4.27.

WO 98/22487 PCT/CA97/00866 -78- Example 12 Synthesis of Na-[2-(1-Thio-p-D-galactopyranosyl) cyclohex-l-yi]-Ltryptophan The title compound was prepared according to procedures D, H and N above using 2-chlorocyclohexan-l-one as the electrophile and L-tryptophan methyl ester as the amino acid ester. Mass spectra data was as follows: M (calcd.): 480.59; M (found): 481.9 505.3 Selected nmr data was as follows: IHnmr (CD30D): 6 4.46 4.40, 4.24 and 4.09.

Example 13 Synthesis of Nc-[4-Phenyl-4-(l-thio- 3 -D-galactopyranosyl)but-2-yl]-L-tryptophan The title compound was prepared according to procedures D, H and N above using 4-phenylbut-3-en-2-one as the electrophile and L-tryptophan methyl ester as the amino acid ester. Mass spectra data was as follows: M (calcd.): 531.64; M (found): 531.3 Selected nmr data was as follows: 'H-nmr (CD 3 OD): 5 4.24 4.23, 4.14 and 4.09.

Example Synthesis of Na-[3-(1-Thio-f-D-galactopyranosyl)cyclohept- -yl]--tryptophan The title compound was prepared according-to procedures D, H and N above using cyclohept-2-en-l-one as the electrophile and L-tryptophan methyl ester as the amino acid ester. Mass spectra data was as follows: M (calcd.): 494.60; M (found): 495.9 Selected nmr data was as follows: 'H-nmr (CD 3 OD): 5 4.50 4.44, 4.41 and 4.32.

WO 98/22487 PCT/CA97/00866 -79- Example 16 Synthesis of Na-[4,4-Dimethyl-3-(1-thio--D-galactopyranosyl)cyclopent-l-yl]-L-tryptophan The title compound was prepared according to procedures D, H and N above using 4 4 -dimethylcyclopent-2-en-l-one as the electrophile and L-tryptophan methyl ester as the amino acid ester. Mass spectra data was as follows: M (calcd.): 494.60; M (found): 495.6 Selected nmr data was as follows: 'H-nmr (CD 3 OD): 6 4.26 4.22, 4.20 and 4.13.

Example 17 Synthesis of Na-[3-(1-Thio-f-D-galactopyranosyl) cyclopent-l-yl]--tryptophan The title compound was prepared according to procedures D, H and N above using cyclopent-2-en-l-one as the electrophile and L-tryptophan methyl ester as the amino acid ester. Mass spectra data was as follows: M (calcd.): 466.55; M (found): 467.9 Selected nmr data was as follows: 'H-nmr (CDO3D): 5 4.33 4.32, 4.30 and 4.23.

Example 18 Synthesis of Na-[4-(l-Thio-f-D-galactopyranosyl)pent-2-yl]-L-tryptophan The title compound was prepared according to procedures D, H and N above using pent-3-en-2-one as the electrophile and L-tryptophan methyl ester as the amino acid ester. Mass spectra data was as follows: M (calcd.): 468.57; M (found): 490.9 Selected nmr data was as follows: 'H-nmr (CD30D): 5 4.30 4.27, 4.22 and 4.09.

WO 98/22487 WO 9822487PCT/CA97/00866 Example 19 Synthesis of Na-[4,4-DimethyI-3-(l-thio-OD-galactopyrnosy).

cyclohex-1-yI-L-tryptophan The title compound was prepared according to procedures D, H and N above using 4,4-dimethylcyclohex-2-en-1I-one as the electrophile and L-tryptophan methyl ester as the amino acid ester. Mass spectra data was as follows: M (calcd.): 508.63; M (found) 512.1 Selected nmr data was as follows: 'H-nmr (CD 3 OD): 4.30 4.26, and 4.21.

Example I110 Synthesis o "f No-3(-hoODglcoyaoy~ylhx1y]Ltytpa The title compound was prepared according to procedures D, H and N above using cyclohex-2-en-l-one as the electrophile and L-tryptophan methyl ester as the amino acid ester. Mass spectra data was as follows: M (calcd.): 480.59; M (found): 483.9 Selected nmr data was as follows: 'H-nmr (CD 3 OD): 6 4.36 4.35, 4.33, and 4.24.

Example J1 Synthesis of Noe-[2- (l-Thio-f 3 -D-galactopyranosyI)cyclopent-1-yI]]L..arginine The title compound was prepared according to procedures D, H and O-above using 2-chlorocyclopentan-i1-one as the electrophile and L-arginine methyl ester -as the amino acid ester. Mass spectra data was as follows: M (calcd.): 436.52; M (found): 436.2 Selected nmr data was as follows: IH-nmr (CD 3 OD): 5 4.54 4.43, 4.41, and 4.28.

WO 98/22487 WO 9822487PCT/CA97/00866 -81- Example J2 Synthesis of Ncr-[ 2 -(1-Thio-3-D-galactopyranosy)cyclohex. -yI]-L-arginine The title compound was prepared according to procedures D, H and 0 above using 2-chlorocyclohexan-1I-one as the electrophile and L-arginine methyl ester as the amino acid ester.. Mass spectra data was as follows: M (calcd.): 450.56; M (found): 453.5 Selected nmr data was as follows: 'H-nmr (CD 3 QD): 5 4.47 4.45, 4.44, and 4.38.

-Example B3 Synthesis of Noa-[ 4 -Pheny-4-(i-Thio-ft-D-galactopyranosyl) but-2-yI]-L--arginine The title compound was prepared according to procedures D, H and 0 above using 2-chlorocyclohexan- 1-one as the electrophile and L-arginine methyl ester as the amino acid ester. Mass spectra data was as follows: M (calcd.): 501.62; M (found): 503.8 Selected nmr data was as follows: IH-nmr (CD 3 OD): 5 4.32 4.31, and 4.30.

Example Synthesis of Not-[ 3 -(l-Thio-fl-D-galactopyranosyI) cyclohept-1-i.y]j 1 arginine The title compound was prepared according to procedures D, H and 0 above using cyclohept-2-en-1-one as the electrophile and L-arginine methyl ester as the amino acid ester. Mass spectra data was as follows: M (calcd.): 464.58; M (found): 467.1 Selected nmr data -was as follows: IH-nmr (CD 3 OD): 5 4.48 4.46, and 4.43.

WO 98/22487 WO 9822487PCT/CA97/00866 -82- Example J6 Synthesis of Noi-[4,4-Dimethyl-3- (1-thio-t%-D-galactopyranosyl)cyclopent-l-yI]-L-arginine The title compound was prepared according to procedures D, H and 0 above using 4,4-dimethylcyclopent-2-en-l1-one as the electrophile and L-arginine methyl ester as the amino acid ester. Mass spectra data was as follows: M (calcd.): 464.58; M (found): 465.-6 Selected nmr data was as follows: 'H-nmr (CD 3 OD): 4.37 4.35, 4.34, and 4.30.

Example P7 Synthesis of Nci-[ 3 -(l-Thio-j%-D-gaIactopyranosyl)cyclopent-1-y]Larginine The title compound was prepared according to procedures D, H and 0 above using cyclopent-2-en-l-one as the electrophile and L-arginine methyl ester as the amino acid ester. Mass spectra data was as follows: M (calcd.): 436.53; M (found): 437.6 Selected nmr data was as follows: 'H-nmr (CD 3 OD): 5 4.37 4.35, and 4.34.

Example J8 Synthesis of Not- (1-Thio-ft-D-galactopyranosyl) pent-2-yl]-L-,arginine The title compound was prepared according to procedures D, H and 0 above using pent-3-en-2-one as the electrophile and L-arginine methyl ester as the amino acid ester. Mass spectra data was as follows: M (calcd.): 438.54; M (found): 437.3 Selected nmr data was as follows: 'H-nmr (CD 3 OD): 5 4.46 4.41, 4.39, and 4.38.

WO 98/22487 WO 9822487PCT/CA97/00866 -83- Example J9 Synthesis of Noa-[4,4-DimethyI-3-(1-thio-i3-D-galactopyranosy).

cyclohex-1-yI]-L-arginine The title compound was prepared according to procedures D, H and 0 above using 4,4-dimethylcyclohex-2-en-1 -one as the electrophile and L-arginine methyl ester as the amino acid ester. Mass spectra data was as follows: M (calcd.): 478.60; M (found): 479.0 Selected nmr data was as follows: 'H-nmr (CD 3 OD): 4.43 4.41, 4.38, and 4.32.

Example 110 Synthesis of Not-[ 3 -(1Thio-O-D-galactopyr anosy1)cyclohex-1-y]I4,arginine The title compound was prepared according to procedures D, H and 0 above using. cyclohex-2-en-lI-one as the electrophile and L-arginine methyl ester as the amino acid ester. Mass. spectra data was as follows: M (calcd.): 450.55; M (found): 451.8 Selected nmr data was as follows: IH-nmr (CD 3 OD): 3 4.34 4.33, 4.32, and 4.29.

Example 1 Synth esis of the Individual Diastereomers of 2,2-Dimnethyl-4-(cyclobut-1-ylamino)cyclopent-1-yl 1-Thio-f-D-galactopyranoside This example illustrates the preparation of individual diastereomers of a compound of formula I.

Step A Synthesis of (1R,S)-2,2-Dimnethylcyclopentan-4-on-1-yI 2,3,4,6- Tetra-0-auroyl-1-thio-%-D-galactopyranoside: To 1 -S-acetyl-2 6-tetra-O-lauryll-thio-i3-D-galactopyranose (5 g, 5 mmol) (from Example C above) and 4,4-dimethyl- 2-cyclopenten-l-one (500 mg, 4.45 mmol) in dry CH 2

CI

2 (10 mL) under argon, was added Et 2 NH (6 mL). After 3 h, the mixture was concentrated and purified by column chromatography (SiO 2 pentane/EtOAc, 9: 1) to give the title compound as a mixture of diastereomers (3.54 g, 66%).

WO 98/22487 PCT/CA97/00866 -84- Step B Separation of the Diastereomers of (1R,S)-2,2- Dimnethylcyclopntan-4-on-1-yI 2,3,4,6-Tetra-O-Iauroyl-1-thio-#l-Dgalactopyranoside: The two diastereomners from Step A (5 g, 4.8 mmol) were separated by column chromatography (SiO 2 pentane/EtOAc, 9: 1) to give (IlS)-2,2dimethylcyclopentan-4-on- l-yl 2,3 ,4,6-tetra-O-lauroyl-l1-thio-1-D-galactopyranoside (428.8 mg, and (1 R)-2 ,2-dimethylcyclopentan-4-on- l-yl 2,3,4 ,6-tetra-O-lauroyl- 1-thio-fl-D-galactopyranoside (373.8 mg, along with a mixture of unresolved compounds (2.74 g, 52%).

Step C Synthesis of (iS, 4RS)- and (1R, 4RS)-2,2-Diinethyl-4hydroxycyclopent-1-yI 2,3,4, 6-Tetra-O:!lauroyI-1-thio-f3-D-galactopyranoside: To each of the purified diastereomers from Step B (in separate reaction flasks) (320 mg, 0.3 mmol) in dry tetrahydrofuran (3 mL), methanol (0.5 mL) and isopropanol (2 mL) under argon atmosphere, was added NaBH 4 (0.12. mmol). After 30 min, AcOH (1 drop) is added and the mixtures were concentrated and the residues dissolved MeOH (2 mL) and added to a column of C-18 silica (5 The columns were washed with MeOH (50 mL) and products eluted pentane (50 mL) to give (IS, 4RS)-2,2-dimethyl- 4-hydroxy-cyclopent- 1-yl 2,3,4, 6-tetra-O-lauroyl-l 1thio-t3-D-galactopyranoside (281 mg, 88 and (LR, 4RS)-2,2-dimethyl-4-hydroxy-cyclopent- l-y 2,3,4, 6-tetra-Olauroy1l- thio-)3-D-galactopyranoside (297 mg, 93 Step D Synthesis of (iS, 4R5)- and (1R, 4RS)-2,2-Dimethyl-4-Omethanesulfonyloxycyclopent-1-yI 2,3,4 ,6-Tetra-O-lauroyl-1-thio-3-Dgalactopyranoside: To each of the (IS, 4RS) and (1R, 4RS) mixtures from Step C (in separate reaction flasks) (280 mg, 0.3 mmol) in dry tetrahydrofuran (2 mL) and dry pyridine (4 mL) under argon atmosphere, was added methanesulfonyl chloride (0.5 mL). After 12 h, the mixtures were washed with 0.5 M HOl and extracted with pentane. After concentration, the residues were purified on C 18-silica (5 g) as described in Step C to afford (iS, 4RS)-2,2-dimethyl-4-Omethanesulfonyloxycyclopent- l-yl 2,3,4, 6-tetra-O-lauroyl- 1-thio-13-Dgalactopyranoside (281 mg, 88%) and 4RS)-2,2-dimethyl-4-O- WO 98/22487 WO 9822487PCT/CA97/00866 methanesulfonyloxycyclopent- 1-yl 2,3,4, 6-tetra-O-lauroyl-l1-thio-O-Dgalactopyranoside (297 mg, 93 as white solids after pentane evaporation.

Step E Synthesis of (1S, (15, (IR, 4S)- and (IR, 4R)-2,2- Dimethyl-4-azidocyclopent-1-yl 2,3,4, 6-Tetra-O-lauroyl-1-thio-fjDgalactopyranoside: To the (1S, 4RS) and (1R, 4RS) mixtures from Step D (in separate reaction flasks) (250 mg, 0.2 mmol) in dry DMF (8 mL) and dry TI{F (3 mL) under argon atmosphere at 60'C was added NaN 3 (340 mg, 5 mmol) and 18 crown-6 (180 mg). After 2 h, the mixtures were concentrated and purified on C18silica (5 g) as described in Step C. Re-chromatography (SiQ pentane/EtOAc, 9:1) permitted the sepdfation of diastereomers to give pure (1S, 4R)-2,2-dimethyl-4azidocyclopent- 1l-yl 6-tetra-O-lauroyl- 1 -thio-,6-D-galactopyranoside (163 mg, (iS, 4S)-2 2 -dimethyl-4-azidocyclopent- l-yl 2,3,4, 6 -tetra-O-lauroyl-l1-thio-fl-Dgalactopyranoside (29 mg, 9 OiR, 4S)-2 2 -dimethyl-4-azidocyclopent- 1l-yl 2,3,4,6- -tetra-O-lauroyl-1-thio-13-D-galactopyranoside (68 mg, and (IR, 4R)-2,2dimethyl-4-azidocyclopent- I1-yl 2,3,4, 6-tetra-O-lauroyl- 1 -thio-1-D-galactopyranoside Step F Synthesis of (iS, (iS, (IR, 4S)- and (1R, 4R)-2,2- Dimnethyl-4-aminocyclopent-i-yl 2,3 4 ,6-Tetra-O-auroyi--thiof3-Dgalactopyran Oside: To each of the four diastereomers of 2,2-dimethyl-4-azidocyclopent-1-yl l-thio-13-D-galactopyranoside from Step E (5 mg, 15 4hmol) in dry isopropanol (1 mL) and dry ethanol (1 mL) under-argon atmosphere, was added NaBH 4 (15 /imol) and NiC1 2 (30 Mmol). After 1 h, the mixtures were neutralized with AcOH (1 drop), concentrated and purified on C18-silica (2 g) as described in Step C to give (1S, (IS, (1R, 4S)- and (IR, 4

R)-

2 ,2-dimethyl-4-aniinocyclopent- 1-yl 1-thio-1-D-galactopyranoside (each 5 mg; quant.).- Step G Synthesis of (IS, (1S, (iR, 4S)- and (1R, 4R)-2,2- DimethyI-4-(cyclobut-1-ylamino)cyclopent.1..yI 2 ,3, 4 ,6-Tetra-O-auroyl-..thio-p.D.

galactopyranoside: To each of four diastereomers of 2,2-dimethyl-4-aminocyclopent-1-yl 1-thio-13-D-galactopyranoside from Step F (in separate reaction flasks) (2 mg, 6.8 jMmol) in dry methanol (1 mL) and dry dichloromethane (1 mL) under argon atmosphere, was added cyclobutanone (250 AL, 3.4 mmol) and sodium WO 98/22487 WO 9822487PCT/CA97/00866 -86triacetoxyborohydride (10 mg, 47 timo1). After 24-48 h, toluene (1 mL) was added and the mixture was concentrated and the residue purified on C 18-silica as described in Step C to give 2.1-2.4 mg (quant.) each of: (1S, 4R)-2 ,2-dimethyl-4-(cyclobut- 1-ylamino)cyclopent- l-yl 1 -thio-fl-Dgalactopyranoside.(B6HA); M (calcd.): 361.50; M (found): 361.6 IH-nmr

(CD

3 5 4.292 (1S, 4S)-2 ,2-dimethyl-4-(cyclobut- 1-ylamino)cyclopent- l-yl 1-thio-O-Dgalactopyranoside (B6HB); M (calcd.)': 361.50; M (found): 361.6 1 1-nmr

(CD

3 OD): 5 4.3 15 (1R, 4S)-2 ,2-dimethyl-4-(cyclobut- 1-ylamino)cyclopent- l-yl 1 -thio-fl-Dgalactopyranoside (B6HC); M'(calcd.): 361.50; M (found): 361.6 'H-nmr

(CD

3 OD): 5 4.300 (1 R, 4R)-2 ,2-dimethyl-4-(cyclobut- 1-ylamino)cyclopent-1-yl 1-thio-j3-Dgala ctopyranoside (B6HD); M (calcd.): 361.50; M (found): 361.6 IH-nmr

(CD

3 OD): e5 4.290 Example 2 Synthesis of 3-Hydroxycyclohex-1-ylI -Thio-ox-L-fucopyranoside The title compound was prepared according to procedures D, E and I above using 1 -S-acetyl-2 6-tetra-O-lauroyl-l1-thio-ca-L-fucopyranose as the thiosaccharide and cyclohex-2-en- 1-one as the electrophile. Mass spectra data -was as follows: M (calcd.): 278.37; M (found): 302.5 Selected nmr data was as follows: 'H-nmr (CD 3 OD): 5 5.43 and 5.38 Example 3 Synthesis of 3-Axninocyclohex-1-yI 1-Thio-ce-L-fucopyranoside The title compound was prepared according to procedure D, F and J above using 1 -S-acetyl-2 6-tetra-O-lauroyl-l1-thio-cr-L-fucopyranose as thethiosaccharide and cyclohex-2-en-l-one as the electrophile. Mass spectra data was as WO 98/22487 PCT/CX97/00866 -87follows: M (calcd.): 277.38; M (found): 278.3 Selected nmr data-;was as follows: 'H-nmr (CD 3 OD): 6 5.43, 5.42, 5.36, and 5.34 Example 4 Synthesis of 3-Acetamidocyclohexyl 1-Thio-ot-L-fucopyranoside The title compound was prepared according to procedures D, F, I and K above using 1 -S-acetyl-2 ,3 ,4 ,6-tetra-O-lauroyl- 1 -thio-a-L-fucopyranose as the thiosaccharide and cyclohex-2-en-1I-one as the electrophile. Mass-spectra data was as follows: M (calcd.): 319.42; M (found): 342.2 Selected nmr data was as follows: 'H-nmr (CD 3 OD): 3 5.43, 5.42, 5.38, and 5.37 Example Synthesis of 3-(2-Carboxybenzamido)cyclohex-1-yI 1-Thio-a-L-fucopyranoside The title compound was prepared according to procedures D, F, G and L above using 1 -S-acetyl-2 6-tetra-O-lauroyl-l1-thio-cr-L-fucopyranose as the thiosaccharide and cyclohex-2-en-1-one as the electrophile. Mass spectra data was as follows: M (calcd.): 425.50, M (found): 448.7 Selected nmr data was as follows: IH-nmr (CD 3 OD): 5 5.48, 5.47, 5.45, and 5.40 Example 6 Synthesis of Noe-(3- (l-Thio-oa-L-fucopyranosy)cyclohex-1-yl]glycine The title compound was prepared according to procedures D, H and M above using 1 -S-acetyl-2 ,3 ,4,6-tetra-O-lauroyl-l1-thio-cx-L-fucopyranose as the thiosaccharide and cyclohex-2-en-1-one as the electrophile and glycine tert-butyl ester as the amino acid ester. Mass spectra data was as follows: M (calcd.): 335.42; M (found): 336.4 Selected nmr data was as follows: 1 H-nmr (CD 3 OD): 6 5.48, 5.47, 5.39, and 5.36 ,0WO098/22487 PTC9/06 PCT/CA97/00866 -88- Example 7 Synthesis of Ni 3 3 -(l-Thio-IvL-uopyranosyl)cycohex1yi.qf..alanine The title compound was prepared according to procedures D, H and M above using 1 -S-acetyl-2 ,3 4 ,6-tetra-O-lauroyl-l1-thio-o -L-fucopyranose as the thiosaccharide and cyclohex-2-en-.1-one as the electrophile and fl-alanine terr-butyl ester as the amino acid ester. Mass spectra data was as follows: M (calcd.): 349.45; M (found): 350.0 Selected nmr data was as follows: 'H-nmr (CD 3 OD): 5.48, 5.47, 5.39 and 5.38 Example 8 Synthesis of Nat-[ 3 -(l-Thio-Li-Iucopyranosyl)cyclohex1y14,..Ieucine The title compound was prepared according to procedures D, H and M above using 1 -S-acetyl-2,3 ,4 ,6-tetra-O-lauroyl- 1 -thio-a-L-fucopyranose as the thiosaccharide and cyclohex-2-en-1-one as the electrophile and L-leucine tert-butyl ester as the amino acid ester. Mass spectra data was as follows: M (calcd.): 391.53; M (found): 392.6 Selected nmr data was as follows: 'H-nmr (CD 3 OD): 6 -5.46, 5.40, and 5.35 Example 9 Synthesis of Na-[3-(l-Thio.a-l.fucopyranosy)cyclohex..1-yl]ILhistidine The title compound was prepared according to procedures D, H and N above using 1 -S-acetyl-2,3 ,4 ,6-tetra-O-lauroy[ 1 -thio-ca-L-fucopyranose as the thiosaccharide and cyclohex-2-en-1-one as the electrophile and L-histidine methyl ester as the amino acid ester. Mass spectra data was as follows: M (calcd.): 415.51; M (found): 418.0 Selected nmr data was as follows: 'H-nmr (CD 3 OD): 5.44, 5.38, and 5.35 WO 98/22487 WO 9822487PCT/CA97/00866 -89- Example Synthesis of Noa-[ 3 -(-Thio-oa-Lfucopyranosy)cycohex-1-y]-L,-tryptophan The title compound was prepared according to procedures D, H and N above using 1-S-acetyl-2,3,4,6-tetra-O-lauroyl-l-thio-ca-L-fucopyranose as the thiosaccharide and cyclohex-2-en-1-one as the electrophile and. L-tryptophan methyl ester as the amino acid ester. Mass spectra data was as follows: M (calcd.): 464.58; M (found): 466.7 Selected nmr data was as follows: 'H-nmr (CD 3 OD): 5.35, 5.32, 5.27, and 5.22 Example 11 Synthesis of Not-[ 3 -(1-Thio-cr-Lfucopyranosyl) cyclohex- 1-yl]-L-arginine The title compound was prepared according to procedures D, H and 0 above using 1 -S-acetyl-2 ,3,4 ,6-tetra-O-lauroyl1-thio-ca-L-fucopyranose as the thiosaccharide and cyclohex-2-en-1-one as the electrophile and L-arginine methyl ester as the amino acid ester. Mass spectra data was as follows: M (calcd.): 434.56; M (found): 435.4 Selected nmr data was as follows: 'H-nmr (CD 3 OD): 5.433, 5.427, 5.38 and 5.32 Example 12 Synthesis of Nor-[3- (5-Acetamido-3 ,5-dideoxy-2-thio-D-glycero-t-D-galacto- 2-nonulopyronosyl)cyclohex-1-yI]-L-histidine The title compound was prepared according to procedures D, H and N above -using methyl-5-acetamido-4 8,9-tetra-O-acetyl-2-S-acetyl-3 ,5-dideoxy-2-thio-Dglycero-ca-D-glacto-2-nonulopyranosonate 2 as the thiosaccharide and cyclohex-2-en-1one as the electrophile and L-histidine methyl ester as the amino acid ester. Mass spectra data was as follows: M (calcd.): 415.51; M (found): 418.0 Selected nmr data was as follows: 'H-nmr (CD 3 OD): 6 5.44, 5.38, and 5.35 WO 98/22487 WO 9822487PCTICA97/00866 Example 13 Attachment of [3-(Carboxybenzamido)norborn-2-yI]methyI 1-Thio-fl-D-galactopyranoside to a Solid Support To [3-(carboxybenzamido)norbom-2-yI] methyl 1 -thio-1-D-galactopyranoside (2.1 mg, 4.5 Amol, from Example D4 above), silyl amninated Chromosorb P (449 mg, prepared as described in U.S. Patent No. 4,137,40111 and Westal et and hydroxybenzotriazole (1.3 mg, 9.4 jsmol) in DMF (1 mL, dried over 4A molecular sieves), was added diisopropylcarbodiimide (1.4 AL, 9.0 Amol). The beads were filtered off after 75 hours, washed with water, DMF, MeOH, and CH 2 Cl 2 To the resulting beads in MeOll (1.5 mL) was added acetic anhydride (0.5 mL) and after 16.5 hours, the beads were filtered and washed with water, DMF, MeOH, CH 2 C1 2 and pentane. Fine particles were removed by suspending the beads in MeON and decanting the supernatant repeatedly. Drying under high-vacuum gave 433 mg of a product having [3-(carboxybenzamido)norbom-2-yl]methyl 1 -thio-j3-Dgalactopyranoside..covalently attached to the Chromasorb P by formatio n of an amide linkage between amine group of the chromasorb P and the carboxy group of the 1thiogalactose derivative -as shown in formula III below. Phenol/11 2 S0 4 assay using the procedure described in M. Dubois et al. 13 showed an incorporation yield of umol/g.

ON1 WO 98/22487 PCT/CA97/00866 -91- Example 14 Attachment of a Thiosaccharide to a Solid Support To a solution of 1, 2 3 4 -di-O-isopropylidene-D-galactopyranose (1 eq.) in pyridine at room temperature is added succinic anhydride (1.2 The reaction is stirred overnight then concentrated in vacuo to give 1, 2 :3,4-di-O-isopropylidene-6-O- 3 -carboxy)propanoyl-D-galactopyranose. To the residue is added 80% aqueous acetic acid to remove the isopropylidene groups. When this reaction is complete, the reaction mixture is concentrated in vacuo and to the residue is added excess 1:1 acetic anhydride/pyridine to afford 1,2,3, 4 -O-acetyl-6-O-(3-carboxy)propanoyl-Dgalactopyranose. To this compound is then added excess thiolacetic acid in dry dichloromethane under argon at 0 0 C and boron trifluoride etherate. The cold-bath is removed after 10 min and after 24 h the mixture is diluted with dichloromethane, washed with saturated sodium bicarbonate, dried over sodium sulfate, and concentrated to afford 1-S-acetyl-2,3, 4 -tri-O-acetyl-6-O-(3-carboxy)propanoyl 1-thiocr-D-galactopyranose. To this compound is added amrninated Merrifield resin and a carbodiimide coupling reagent to afford the O,S-protected galactopyranose coupled to the resin through the 6-O-(3-carboxy)propanoyl group.

Example Solid-Phase Synthesis of 1-Thiogalactose Derivatives The example illustrates the solid-phase synthesis of 1-thiogalactose derivatives of formula I.- Step A Synthesis of 1-Dithioethyl-2,3,4,tetra-O-acetyl-galactopyranoside: 1-Thio- 2 ,3,4,6-tetra-O-acetyl-galactopyranoside (500 mg, 1.37 mmol) and diethyl-Nethyl-sulfenylhydrazodicarboxylate (360 mg, 2.0 mmol) (prepared as described in T.

Mukaiyama 2 are dissolved in dichloromethane (14 mL) and stirred at room temperature. After 10 min, the solution is concentrated and column chromatography (SiO 2 hexane/ethylacetate 2:1) yields 1-dithioethyl-2,3,4,6-tetra-O-acetylgalactopyranoside (580 mg, quant) as a white solid 0.27 in hexanes/ethyl acetate 1 k WO 98/22487 PCT/CA97/00866 -92- 'H-NMR (360 MHz, CHC1 3 5 1.30 (dd, 3 H, J 7.4 Hz, CH 3 1.96, 2.02, 2.03, 2.13 (4 s, 12 H, 4 CH 3 CO), 2.79 (ddd, 2 H, J 7.4Hz, J 7.4 Hz, J 1.3 Hz, CHz), 3.94 (ddd, 1 H, J 4 .5 1.0 Hz, JS,6, 6.6 Hz, J,6, 7.6 Hz, 4.10 (ddd, 2 H, 61-H, 6b-H), 4.51 1 H, JI.2 10.0 Hz, 5.05 (dd, 1 H, J2.3 10.0 Hz, J 3 4 3.3 Hz, 5.38 (dd, 1 H, J 1 .2 10.0 Hz, J 3 10.0 Hz, 5.40 (dd, 1 H, J 3 ,4 3.3 Hz, J4.

5 1.0 Hz, m/z calcd. for C 16 H2OS, (M+Na) 447.1, found .447.0.

Step B Synthesis of 1-Dithioethyl-1-D-galactopyranoside: 1-Dithioethyl- 2 3 ,4, 6 -tetra-O-acetyl-galactopyranoside from Step A (500 mg, 1.18 mmol) was dissolved in dry methanol (10 mL) and treated with methanolic sodium methoxide (1 M, 150 iL). After 2 h, the solution was neutralized with Amberlite 1R-120 (H resin, filtered and concentrated to give l-dithioethyl-6-0-D-galactopyranoside as a white solid (300 mg, quant).

Step C Coupling of 1-Dithioethyl--D-galactopyranoside to a Resin: 1- Dithioethyl-6-0-D-galactopyranoside (200 mg, 780 Amol) was dissolved in dry pyridine (8 mL). Trityl chloride-resin (1 g, 950 pmol trityl chloride resin, loading 0.95 mmol/g of active chlorine, polymer matrix: copolystyrene-l% DVB, 200-400 mesh, Novabiochem) and DMAP (5 mg) were added and the mixture was heated for 24 h at 60*C. The resin was filtered off, and washed successively with methanol, tetrahydrofuran, dichloromethane and diethyl ether (10 mL each) to afford 1dithioethyl-O-D-galactopyranoside covalently linked to the trityl resin through the hydroxyl group in the 6-position.

Step D Generation of the Free Thiol on the Resin: The resin from Step C mg) is swollen in dry tetrahydrofuran (1.5 mL). Dry methanol (300 dithiothreitol (74 mg) and triethylamine (180 pL) are added and the mixture is shaken for 10 hours at room temperature. The resin is filtered off and washed successively with methanol, tetrahydrofuran, dichloromethane and diethyl ether (10 mL/each). IR (of intact beads): 2565 cm-' (SH stretch).

Step E Michael Addition Reaction: The resin from Step D (50 mg) was swollen in dry N,N-dimethylformamide (1 mL) and then cyclohept-2-en-1-one (70 /l, 63 /mol) was added and the mixture was shaken at room temperature. After 2 hours, WO 98/22487 PCT/CA97/00866 -93the resin was filtered off and washed successively with methanol, tetrahydrofuran, dichloromethane and diethyl ether (10 mL each).

Step F Reductive Amination with an Amino Acid: The resin from Step E mg) was swollen in dichloromethane (1 mL). Glycine tert-butyl ester hydrochloride (75.mg, 447 /tmol), sodium sulfate (100 mg), sodium triacetoxyborohydride (63 mg, 297 /mol) and acetic acid (10 were added at room temperature under argon atmosphere and the mixture shaken for 24 hours. The resin was then filtered off and washed successively with water, methanol, tetrahydrofuran and dichloromethane.

Step G Cleavage from the 1-Thiogalactose Derivative from the Resin and Deblocking of the Amino Acid Ester: The resin from Step F (50 mg) was shaken with trifluoroacetic acid (1 mL) and triisopropylsilane (20 AL) in dichloromethane (2 mL) at room temperature. After 3 hours, the resin was removed by filtration and washed with dichloromethane (10 mL). After adding toluene (10 mL), the solution was concentrated, then co-evaporated twice with toluene. The residue was dissolved in water (1 mL) and applied onto two C 8 g-Sep-Pak-cartridges (Waters Sep-Pak Plus).

The C 1 g silica was washed with water (4 mL) and the final product was eluted with methanol and concentrated. After freeze drying from 5 mL of water, Nc-[3-(1thio-(3-D-galactopyranosyl)cyclohept-l-yl]glycine was obtained as a white powder (4.8 mg). The diastereomers ratio was 10:10:8:6 as determined by 'H-NMR.

'H-NMR (360 MHz, CD 3 0D, anomeric protons): 6 4.36 J,.2 9.6 Hz), 4.40 (d, J =2 9.5 Hz), 4.44 J1,2 9.1 Hz), 4.45 J,2 9.2 Hz); m/z calcd. for CisHvNO 7 S 366.2, found 366.1.

Example 16 Inhibition of Heat-Labile Enterotoxin Binding to GDIb In this example, 1-thiogalactose derivatives of formula I above were tested for their ability to inhibit the binding of heat-labile enterotoxin from E. coli to ganglioside -GDtb. This bioassay was conducted using the procedure described by A.-M.

Svennerholm 2 1 except that ganglioside GoDb was used instead of ganglioside GMI. The compounds of Examples Al, A2, A4-A7, AlO, All, Bl, B2, B4-B7, B10, Bll, C2, WO 98/22487 PCT/CA97/00866 -94- C7, C10, Cll, D2, D4, D5, El, E2, E4, E10, Ell, Fl, F2, F5, F7, Fi0, Fl1, G2, G5, 12, 15, and J7 were tested in this bioassay. All of the compounds tested inhibited binding of heat-labile enterotoxin to ganglioside GDIb by at least 20%, except for the compounds of Examples A2, A5, A7, C10, D2 and G2, which did not inhibit binding by at least 20% at the concentration employed in the assay.

Example 17 Inhibition of Cholera Toxin Binding to GDlb In this example, 1-thiogalactose derivatives of formula I above were tested for their ability to inhibit the binding of cholera toxin to ganglioside GDIb. This bioassay was conducted using the following modification of the procedure described by A.-M.

Svennerholm" 1 On day 1, microtiter plates (C96 Maxisorp) were coated with 100 /tL of 1 mg/mL GDlb (disialoganglioside GDlb, MW 2127, Fluka) in PBS per well and incubated overnight at 37"C.

On day 2, the samples to be tested were diluted in BSA-Tween-PBS

BSA

and 0.05% Tween-20 in PBS; Sigma). A total of 500 /L of each solution was prepared so that each point could be measured in quadruplicate. A concentration curve of 10, 20 and 30 ng/mL of CTB5-HRP (CT-B5 conjugated to HRP, Sigma, lyophilized, diluted in Tween-PBS) was prepared. For the inhibition experiments, ng/mL of CTB5-HRP was used. The samples were then incubated for 2 hours at room temperature. After incubation, the plates were emptied and unattached ganglioside was removed by washing the plates 2 times with 200 /L PBS per well.

Additional binding sites on the plastic surface were then blocked by incubating the plates with 200 yL of 1 BSA in PBS per well for 30 minutes at 370C. The plates were then emptied and unattached BSA was removed by washing the plates 3 times with 200 AL of 0.05% Tween 20-PBS per well. Samples (100 were added to 4 different wells and incubated for 30 minutes at room temperature. The plates were S emptied and unattached BSA was removed by washing the plates 3 times with 200 AL of 0.05% Tween 20-PBS per well.

WO 98/22487 PCT/CA97/00866 A substrate solution was freshly prepared for each ELISA. Each solution contained 10 mg of o-phenylenediamine (Sigma), 5 mL of 0.1M sodium citrate (filter sterile or autoclaved), 5-mL of 0.1 M citric acid (filter sterile or autoclaved) and 4 mL of 30% H 2 0 2 (Gloves should be worn since o-phenylenediamine is carcinogenic).

The substrate solution (100 ML) was then added to each well and incubated for minutes at room temperature. After incubation, the OD 45 0 was recorded. Under the conditions of the assay, D-galactose had an IC50 of 30 mM.

The compounds of Examples A1-A10, B1-B6, B6A-B6L, B6Q, B6T, B7-B8, B1,_C1-C3, C5, C7, C8, C10, D1-D5, D8, E1-E9, F1-F10, G2, G3, G5-G10, H2, H3, H5-H10, 11-13, 15-110, J1-J3 and J5-J10 were tested in this bioassay. All of the compounds tested inhibited binding of cholera toxin to ganglioside GDib by at least except for the compounds of Examples Al, A3, A4, A6-A8, A10, B1, B3, B4, C1, C3, C8, D3, E5, E8, E9, Fl, F5-F7, F9, F10, G3, G7-G10, H2, H5, H8- 12, 18-110, J5-J10, which did not inhibit binding by at least 20% at the concentration employed in the assay 1 mg/mL).

Example 18 Neutralization of the Cytotonic Activity of CT and LT In this example, the solid support material of Example 13 was tested for its ability to neutralize the cytotonic activity of CT and LT. The cytotonic activity of CT and LT was measured by the use of Chinese hamster ovary cells (CHO) that were maintained in Hams F12 media supplemented with 10% fetal bovine serum (FBS) in an atmosphere of 5% CO 2 at 37"C. Toxin samples were diluted 1:5 in Hams media and filter sterilized through 0.22 micron syringe filters. Samples were then serial fold diluted in media and 100 tL of each dilution was added to wells with confluent monolayers of CHO cells and incubated for 24 h at 37*C (under 5% CO). Each sample was analyzed two times. Cytotonic effects were readily visible after 24 h incubation by comparing wells with controls that do not contain toxin. After 24 h, the cells were fixed with 95% methanol and stained with Geimsa stain. Toxin containing samples from neutralization experiments were treated in an analogous fashion except that the percent neutralization was determined by comparing the WO 98/22487 PCT/CA97/00866 -96endpoint dilutions of samples with and without the solid support material of Example 13.

A solution containing purified CT or LT 10 or 20 1g in 1 mL PBS) was added to the solid support material of Example 13 (20 mg) in 1.5 mL microcentrifuge tubes and incubated at room temperature for 1 h on an end-over rotator. After incubation, the solid support material was allowed to settle to the bottom of the tubes and the supernatants were carefully removed. The supernatants were added to CHO cells and the cytotonic endpoint determined after incubation for 24 h as described above. The extent of reduction in the endpoint in the presence of the solid support material was determined by comparing with controls in which solid support material was not added.

Results showed that the solid support material of Example 13 neutralized more than 90% of CT and LT activity, regardless of toxin concentration, less than toxin activity remained.

Example 19 Inhibition of Colonization Factor Antigens (CFA pili) Binding to Glycophorin In this example, 1-thiogalactose derivatives of formula I above were tested for their ability to inhibit CFA pili binding to glycophorin. Bacterial surface adhesion antigens such as CFA pili are a virulence factor expressed by certain enteric pathogens, including enterotoxigenic E. coli. These pili are important factors in bacterial attachment to cell surface receptors. Accordingly, inhibition of CFA pili binding is a useful test to determine whether a compound will inhibit the binding of a pathogenic microorganism to cell surface receptors.

Binding assays were done by coating microtitre wells with 50 AL of glycophorin g/mL) in PBS for 2 h at 37"C. The solution was removed by aspiration and replaced with 100 pL of 1% BSA in PBS containing 0.05% Tween 20 (PBST) and incubated at 37*C for an additional 1 h. The microtitre wells were washed three times with 200 /L of PBST and then replaced with biotinylated CFA I (5 ug/mL) in 4L of PBS containing 0.05% BSA. After incubating for 2 h at 37"C the binding -97reaction was stopped by aspirating the solutions and the plate was washed with PBST (3 X 200 Avidin-peroxidase (50 pL of a 1/3000 dilution of a i mg/mL solution in PBST containing 0.05 BSA) was added and the plates were incubated for an additional 1 h. After washing the wells as described above, 100 AL of the substrate solution (0.42 mM tetramethylbenzidine (TMB) in 0.1 M sodium citrate buffer, pH containing 0.5 /M urea peroxide) was added and the plates were incubated for min at ambient temperature and the enzyme reaction stopped by adding 50 LL of 2N H1SO 4 Binding assays were done in triplicate and background binding was measured in wells coated with BSA only.

Binding inhibition assays were done using oligosaccharide analogs at a concentration of 1 mg/mL in PBS. Inhibitors were preincubated with biotinylated CFA I pili (5 p/mL) for 1 h at 37*C prior to adding to glycophorin-coated microtitre wells as outlined above. o-Nitrophenyl-fl-D-galactose was utilized as a control S inhibitor for these experiments.

15 The 1-thiogalactose derivatives of Examples Al-A10, B1-B8, B10, C1-C3, C7, C8, C10, D1-D5, D8, D10, E1-E10, F1-F10, G1-G3, G5-G10, H1-H3, H5-H10, 11-13, 15-110, J1-J3 and J5-JO1 were tested. Of these compounds, the results showed i that the compounds of Examples B2, B5, H2, H3, H5, H6, H7, H8, H9, H10, II, 12 and J9 inhibited CFA I pili binding to glycophorin, with the amount of inhibition 20 ranging from 13 to 71%. The compounds having a histidine or a tryptophan (Group S H and I) moiety were particularly good inhibitors in this experiment.

I From the foregoing description, various modifications and changes in the composition and method will occur to those skilled in the art. All such modifications coming within the scope of the appended claims are intended to be included therein.

0o 2 Throughout this specification and the claims which follow, unless the context requires otherwise, the word "comprise", and variations such as "comprises" and "comprising", will be understood to imply the inclusion of a stated integer or step or group of integers or steps but not the exclusion of any other integer or step or group of S .integers or steps.

PAOPER\Kbm\5044-iO9S spe.doc.24/5A)I -97A- The reference to any prior art in this specification is not, and should not be taken as, an acknowledgment or any form of suggestion that that prior art forms part of the common general knowledge in Australia.

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Claims (15)

1. A method for synthesizing a thiosaccharide derivative, which method comprises: providing a thiosaccharide; providing at least a stoichiometric amount of a coupling reagent selected from the group consisting of Michael acceptors, a-(sulfonic ester) carbonyl compounds and a-halocarbonyl compounds; and contacting the thiosaccharide and the coupling reagent under conditions which provide for a thiosaccharide carbonyl compound.

2. The method of claim 1, which method further comprises the step of: reducing the carbonyl group of the thiosaccharide carbonyl compound to form a group selected from hydroxy and amino derivatives.

3. A method for synthesizing a thiosaccharide derivative on a solid support, which method comprises: S 15 providing a thiosaccharide; i providing at least a stoichiometric amount of a coupling reagent selected from Michael acceptors, a-(sulfonic ester) carbonyl compounds and a-halocarbonyl compounds wherein either the thiosaccharide or the coupling reagent is covalently attached to a solid support; and 20 contacting the thiosaccharide and the coupling reagent under conditions which provide for a thiosaccharide carbonyl compound covalently attached to a solid support.

4. The method of claim 3, which method further comprises the step of: reducing the carbonyl group of the thiosaccharide carbonyl compound to form a group selected from hydroxy and amino derivatives.

5. A method for preparing a thiosaccharide derivative library produced by z; synthesizing on each of a plurality of solid supports a single compound wherein each r of compound comprises a thiosaccharide derivative, which library is synthesized in a process comprising: a) apportioning solid supports among a plurality of reaction vessels which supports comprise a reactive functional group covalently bound thereto which group is capable of covalently binding a thiosaccharide at a position other than the thiol group; b) contacting the supports in each reaction vessel with a unique thiosaccharide under conditions wherein the thiosaccharide is covalently attached to the solid supports through the reactive functional group; c) pooling the supports; d) apportioning the supports from above among a plurality of reaction vessels; and e) contacting the supports in each reaction vessel from above with a unique coupling reagent selected from the group consisting of Michael acceptors, a-(sulfonic ester) carbonyl compounds and a-halocarbonyl compounds under conditions which provide for a thiosaccharide carbonyl compound covalently bound to said support.

6. The method of Claim 5, which method further comprises the steps of: pooling the supports from procedure apportioning the supports from above among a plurality of reaction vessels; and 20 reducing the carbonyl group of the thiosaccharide carbonyl compound to form a group selected from hydroxy and amino derivatives.

7. The method of Claim 6, which method further comprises the steps of: pooling the supports from procedure above; apportioning the supports from above among a plurality of reaction vessels; and derivatizing the hydroxyl or amine groups to form a functional group selected from esters, substituted amines, amides, carbamates, ureas, thioureas, t~ki i oesters and thiocarbamates. -100-

8. A method for preparing a thiosaccharide derivative library produced by synthesizing on each of a plurality of solid supports a single compound wherein each compound comprises a thiosaccharide derivative, which library is synthesized in a process comprising: a) apportioning solid supports among a plurality of reaction vessels which supports comprise a reactive functional group covalently bound thereto which group is capable of covalently binding a coupling reagent; b) contacting the supports in each reaction vessel with a unique coupling reagent selected from the group consisting of Michael acceptors, a-(sulfonic ester) carbonyl compounds and a-halocarbonyl compounds under conditions wherein the coupling reagent is covalently attached to the solid supports through the reactive functional group; c) pooling the supports; d) apportioning the supports from above among a plurality of reaction of*: vessels; and 15 e) contacting the supports in each reaction vessel from above with a unique thiosaccharide under conditions which provide for a thiosaccharide carbonyl compound covalently bound to said support.

9. The method of Claim 8, which method further comprises the steps of: .20 pooling the supports from procedure apportioning the supports from above among a plurality of reaction vessels; and 0 reducing the carbonyl group of the thiosaccharide carbonyl compound to form a group selected from hydroxy and amino derivatives. The method of Claim 9, which method further comprises the steps of: pooling the supports from procedure above; apportioning the supports from above among a plurality of reaction ,7,yessels; and WO 98/22487 PCT/CA97/00866 -101- derivatizing the hydroxyl or amine groups to form a functional group selected from esters, amides, carbamates, ureas, thioureas, thioesters and thiocarbamates.

11. A library of diverse thiosaccharide derivatives comprising a plurality of solid supports having a plurality of covalently bound thiosaccharides derivatives, wherein the thiosaccharide derivative bound to each of said supports is substantially homogeneous and further wherein the thiosaccharide derivative bound on one support is different from the thiosaccharide derivatives bound on the other supports and further wherein said thiosaccharide derivative is represented by the formula Saccharide Y wherein R' is selected from the group consisting of hydrogen, alkyl, substituted alkyl, alkenyl, alkaryl, alkoxyalkyl, aryl, cycloalkyl, cycloalkenyl, heteroaryl, heterocyclic, thioalkoxyalkyl and a linking arm covalently linking the compound of formula I to the support; R 2 is selected from the group consisting of hydrogen, alkyl, substituted alkyl, alkenyl, alkaryl, alkoxyalkyl, aryl, cycloalkyl, cycloalkenyl, heteroaryl, heterocyclic, -102- thioalkoxyalkyl and a linking arm covalently linking the compound of formula I to the support; R 3 is selected from the group consisting of hydrogen, alkyl, substituted alkyl, alkenyl, alkaryl, alkoxyalkyl, aryl, cycloalkyl, cycloalkenyl, heteroaryl, heterocyclic, thioalkoxyalkyl and a linking arm covalently linking the compound of formula I to the support; or R' and or R' and R 3 or R 2 and R 3 or R 2 and R 3 can be joined, together with the carbon atoms to which R' and/or R 2 and/or R 3 are attached, to form a cycloalkyl, cycloalkenyl or heterocyclic ring; R 4 is selected from the group consisting of-XR 5 -XC(W)R 6 and -XC(W)X'R 7 wherein W is selected from the group consisting of oxygen, sulfur and NH; and X and X' are each independently selected from the group consisting of oxygen, sulfur and -NR 9 wherein R 9 is selected from the group consisting of hydrogen and alkyl; or when R 4 is -XR 5 and R 5 is not hydrogen, X can :15 also be selected from the group consisting of and -SO-; R is selected from the group consisting of hydrogen, alkyl, alkenyl, alkaryl, alkoxyalkyl, aryl, cycloalkyl, cycloalkenyl, heteroaryl, heterocyclic, thioalkoxyalkyl and a linking arm covalently linking the compound of formula I to the support, and when .20 X is -NR 9 then R 5 together with X can form an amino acid selected from the group consisting of glycine, p-alanine, leucine, histidine, tryptophan and arginine; or R 5 and R', S or R 5 and R 2 or R 5 and R 3 can be joined, together with X of the -XR 5 group and the carbon atoms to which R 1 and/or R 2 and/or R 3 are attached, to form a heterocyclic ring; R 6 is selected from the group consisting of alkyl, alkenyl, alkaryl, alkoxyalkyl, aryl, cycloalkyl, cycloalkenyl, heteroaryl, heterocyclic, thioalkoxyalkyl and a linking arm covalently linking the compound of formula I to the support; or R 6 and or R 6 and R 2 or R 6 and R 3 can be joined, together with the moiety of the R XC(W)R 6 group and the carbon atoms to which R' and/or R 2 and/or R 3 are attached, Sform a heterocyclic ring; ;J R 7 is selected from the group consisting of alkyl, alkenyl, alkaryl, alkoxyalkyl, T 'O I1, cycloalkyl, cycloalkenyl, heteroaryl, heterocyclic, thioalkoxyalkyl and a linking WO 98/22487 PCT/CA97/00866 -103- arm covalently linking the compound of formula I to the support; or R 7 andRI, or R 7 and R 2 or R 7 and R 3 can be joined, together with the moiety of the XC(W)X'R 7 group and the carbon atoms to which R' and/or R 2 and/or R 3 are attached, to form a heterocyclic ring; R 8 is selected from the group consisting of alkyl, alkenyl, alkaryl, alkoxyalkyl, aryl, cycloalkyl, cycloalkenyl, heteroaryl, heterocyclic, thioalkoxyalkyl and a linking arm covalently linking the compound of formula I to the support; or R 8 and or R 8 and R 2 or R' and R 3 can be joined, together with the moiety of the C(W)XR 8 group and the carbon atoms to which R 2 and/or R 3 are attached, to form a heterocyclic ring; Y is selected from the group consisting of sulfur, and n is an integer equal to 0 or 1; and pharmaceutically acceptable salts thereof; wherein the saccharide is selected from the group consisting of a monosaccharide, an oligosaccharide, monosaccharide-Z- and oligosaccharide-Z-, wherein Z is a linking arm covalently linking the compound of formula I to the solid support; with the proviso that only one of RI, R 2 R 3 R 4 R 6 R 7 R 8 and Z is linked to the solid support.

12. The library of Claim 11 wherein the compound of formula I is an a- anomer.

13. The library of Claim 11 wherein the compound of formula I is a f- anhoier.

14. The library of Claim 11 wherein, when n is 0, R' aid R 2 are joined, together with the carbon to which they are attached, to form a cycloalkyl ring having to 7 carbon atoms optionally substituted with 1 to 3 alkyl groups. P:'OPER\Kbm50440-98 spe.doc-24.05.01

104- The library of Claim 14, wherein R' and R 2 are joined, together with the carbon to which they are attached, to form a cyclopentane or cyclohexane ring. 16. The library of Claim 11 wherein, when n is 1, R' and R 2 are joined, together with the carbon atoms to which R 2 and R 3 are attached, to form a cycloalkyl ring having 5 to 7 carbon atoms optionally substituted with 1 to 3 alkyl groups. 17. The library of Claim 16 wherein R' and R 2 are joined, together with the carbon atoms to which R 2 and R 3 are attached, to form a cyclopentane, dimethylcyclopentane, cyclohexane, dimethylcyclohexane or cycloheptane ring. 18. The library of Claim 16 wherein R 4 is -XR 5 where X is -NH- and R 5 is 10 cycloalkyl. 19. The library of Claim 11 wherein, when n is 1, R 2 and R are joined, together with the carbon atoms to which they are attached, to form a norbomene ring. 20. The library of Claim 11 wherein R 4 is -XR 5 where X and R 5 form an amino group, a hydroxy group or an amino acid selected from the group consisting of glycine, f- 15 alanine, leucine, histidine, tryptophan and arginine. 21. The library of Claim 11 wherein R 4 is -XC(O)R 6 where X is -NH- and R 6 is methyl or 2-carboxyphenyl. 22. A method for synthesizing a thiosaccharide derivative according to Claim 1, a a substantially as hereinbefore described with reference to the Examples. 23. A method for synthesizing a thiosaccharide derivative on a solid support according to Claim 3, substantially as hereinbefore described with reference to the Examples. 2 Rai 24. A method for preparing a thiosaccharide derivative library according to Claim B 5 or Claim 8, substantially as hereinbefore described with reference to the Examples. A library of diverse thiosaccharide derivatives according to Claim 11, P:\OPER\Kbm50440-98 spe.doc-24A05 I

105- substantially as hereinbefore described with reference to the Examples. 26. A thiosaccharide derivative synthesized by a method according to any one of Claims 1 to 4, 22 or 23. 27. A thiosaccharide derivative library prepared by the method of any one of Claims 5 to 9 or 24. DATED this 24th day of May, 2001 Synsorb Biotech Inc. By DAVIES COLLISON CAVE Patent Attorneys for the Applicants S ee o