AU722742B2 - Methods for making encapsulated microspheres from heat denatured protein using mechanical cavitation - Google Patents

Description

S.

Si S

AUSTRALIA

Patents Act 1990 MOLECULAR BIOSYSTEMS, INC.

ORIGINAL

COMPLETE SPECIFICATION STANDARD PATENT Invention Title: Methods for making encapsulated miicrospheres from heat denatured protein using mechanical cavitation The following statement is a full description of this invention including the best method of performing it known to us:- 1/1 METHODS FOR MAKING ENCAPSULATED MICROSPHERES FROM HEAT DENATURED PROTEIN USING MECHANICAL CAVITATION Description Technical field This invention relates to ultrasonic imaging agents composed of proteinaceous microspheres encapsulating insoluble gases and methods for their production and use.

Background Diagnostic ultrasonic imaging is based on the principle that waves of sound energy can be focused upon an area of interest and reflected in such a way as to produce an imnage thereof. An ultrasonic scanner is placed on a body surface overlying the area to be imaged, and ultrasonic energy in the form of sound waves are directed toward that area. The scanner detects reflected sound waves and translates the data into video images. When eniergy is transmnitted through a substance, the amount of energy reflected depends upon the velocity of the transmission and the acoustic properties of the substance. Changes in the substance's acoustic properties variations in acoustic impedance) are most prominent at the interfaces of different acoustic densities, such as liquid-solid or liquid-gas.

Consequently, when ultrasonic energy is directed through tissue, organ structures generate sound-reflection on signals for detection by the ultrasonic scanner. These signals can be intensified by the proper use of a contrast agent.

Ultrasound imaging agents of particular importance employ the use of gas because of its efficiency as a reflector of ultrasound. Resonant gas bubbles scatter sound a thousand times more efficiently than a solid particle of the same size. Ophir and Parker describe two types of gas-containing imaging agents as being: free air bubbles and encapsulated air bubbles (Ultrasound in Medicine and Biology 15(4):319-333, 1989) However, free gas bubbles of the appropriate size are too short-lived to be effective for most Ill vivo applications (Meltzer, et al., Ultrasound in Medicine and Biology 6:263-269, 1980). Ophir and Parker point out that the development of encapsulated gas bubbles was an attempt to overcome this problem.

The second major class of gas-containing ultrasound contrast agents described by Ophir and Parker are the encapsulated microbubbles, hereinafter referred to as "microspheres". The gas bubble is surrounded by a shell composed of a protein or other biocompatible material. A current commercial microsphere contrast agent is ALBUNEX®. (Molecular Biosystems, Inc., San Diego, CA) which is composed of human serum albumin encapsulated air microspheres. See U.S. Patent Nos. 4,572,203 and 4,844,882.

Air microspheres have been shown to quickly lose echogenicity when subjected to pressures of 150 mm Hg, such as would be encountered during injection and circulation in vivo (dejong, N. et al., Ultrasound Med. Biol.

19:279-288, 1993). Present encapsulating technology has yet to produce a material suitable as an ultrasound contrast agent that will survive long enough in vivo for most desired applications. In fact, an agent capable of imaging the myocardial wall must withstand transient pressures pulses of at S**least 250 mm Hg (about 5 psig).

In an effort to solve the pressure-instability problem of microspheres, recent teachings have centered on improving the shell, because, it is believed, the microsphere shells or "membranes" are too fragile or brittle under pressure, resulting in rapid collapse in vivo. Giddey (PCT/EP91/01706; PCT 92/05806) stated, "because of their rigidity, the membranes cannot sustain sudden pressure variations to which the microspheres can be 25 subjected, for instance during travel through the bloodstream, these variations or pressure being due to heart pulsations." To overcome shell rigidity, he proposed to pre-emulsify air in a protein solution containing a large percentage of a viscosifying agent (40% 80% polyols) and subject it to mechanical shear in a high speed blender. Bubbles of the appropriate size are collected and coated with a suitable surfactant to stabilize them in a soft shell.

Holmes (PCT WO 92/17213) proposed to enhance the in vivo stability of protein microspheres by strengthening the shell with biodegradable chemical crosslinking reagents.

Bichon et al. (EPA 90/810367) and Schneider et al. (Inv. Radiol.

27:134-139, 1992) describe the production of porous (5 to 2000 nm pore size) polymeric "microballoons." They report in the European Patent Application that "the microporous structure of the microballoon's envelope is a factor of resiliency, the microspheres can readily accept pressure variation without breaking." Erbel and Zotz Patent No. 5,190,982) describe a cross-linked polymeric microcapsule in which air is entrapped.

Schneider et al. (EPA 554, 213) indicates that the pressure resistance of microspheres can be improved by having at least a portion of the gas that is encapsulated be a gas which has a SgJMWgs 0.0031, where Sgs is the water solubility of the gas in liters/liter and MWgas is the average molecular weight of the gas in daltons. Table 1 of the reference lists N 2

SF

6 CBrF 3 and

CF

4 as meeting this criterion. The reference teaches that these microspheres may be made in either of two ways. The first is a two-step method in which air-containing microspheres are prepared by a known method and the air is replaced with the insoluble gas by a gas-exchange method, by incubating the air-filled microspheres under an atmosphere of the insoluble gas for an i adequate time.

S* The second is a one-step method in which microspheres are made by the method of EPA 324,938 (See Example 1 of the reference) using an 20 insoluble gas instead of air. In that method, the gas was passed over a solution of a shell-forming material a solution of albumin) while a sonicator horn was lowered into the vessel and then removed.

Unfortunately, neither of these methods is practically useful for making stable suspensions of protein encapsulated insoluble gas-filled 25 microspheres. Using the first (two-step) method, only a small number of airfilled albumin microspheres can survive the exposure to an insoluble gas environment (the second step of the two-step method). The efflux of soluble gases (air) out of the microspheres is greater than the influx of insoluble gas into the microspheres which results in complete collapse of the microspheres, leaving only shell debris. This effect is particularly pronounced with the more insoluble gases such as perfluoroethane. The second process produces microspheres which lose volume as pressure is applied and do not exhibit recovery after the release of pressure. It is believed that both of these processes produce inferior microspheres because the microspheres contain significant amounts of air and that the presence of air during formation may lessen the benefits of producing the microspheres in the presence of insoluble gas alone. It is noted, in this regard, that prior investigators have considered the presence of oxygen essential in making microspheres by cavitation.

Suslick reported that ultrasound-associated cavitation was suitable as a method of production of microspheres only in the presence of oxygen. In detailed studies, Suslick et al., (Proc. Natl. Acad. Sci. 88:7708-7710, 1991; J.

Am. Chem. Soc. 112:7807-7809, 1990) reported that oxygen participates in the cavitation-induced intermolecular rearrangement of disulfide bonds needed for a stable protein shell. Suslick states "We find that microcapsule formation is strongly inhibited by the absence of 02". He goes on to state, "If the reaction is run under an inert atmosphere (He, Ar or N2), microcapsules are not formed." "Experimentally, high concentrations of microbubbles are synthesised only when the reaction is run under 02 or air. See also, U.S.

4,774,958. The previous belief that air was necessary for the formation of albumin microspheres was also noted in Holmes (PCT WO 92/17213).

Therein, the production of microspheres containing various low molecular weight gases was disclosed. However, when describing the production of albumin microspheres by sonication, the authors stated, "Another wellestablished method described, US-A-4,774,958, for creating a gas- 20 containing bubble is by sonication of the mixture in the presence of air." (Emphasis added.) In one aspect, the current invention relates to the unexpected finding that high concentrations of proteinaceous microspheres, entrapping relatively insoluble gas, can be made by ultrasound or mechanical cavitational 25 processes in the presence of the insoluble gas without the presence of oxygen. Such microspheres exhibit surprising and greatly improved stability and elasticity to applied pressure, with better or equivalent echogenicity.

The proteinaceous shell prevents coalescence and resists expansion due to diffusion of dissolved atmospheric gases from the surrounding environment.

In another aspect, the current invention relates to a novel procedure for the production of protein-shelled microspheres which employs mechanical energy in the form of shear forces. These forces are responsible for mechanically shearing a liquid-gas mixture to form a microbubble suspension, and also for causing hydrodynamic cavitation which releases energy. This energy can be absorbed by the surrounding liquid to effect localized protein denaturation and deposition at a gas-liquid interface to form discrete mnicrospheres. Hydrodynamnic cavitation can be distinguished from ultrasonic (acoustic) cavitation on the basis of the way in which each produces pressure variations on liquid systems which lead to the release of energy. In the former, pressure variations are produced by the rapid flow of a liquid through an orifice or across a surface, whereas in the latter, cycles of high frequency sound waves produce rapid local pressure variations. (See F. Ron Young. 1989 Cavitation Pages 4-5, McGraw-Hill Book Co. London).

Additionally, hydrodynamic cavitation is produced in a flowing liquid, a liquid which is flowing through or across a stationary object. In comparison.

acoustic cavitation is produced in a liquid system which must remain stationary through enough cycles of increasing and decreasing pressure (positive and suction pressure) to exhibit cavitation. Even in a continuous flow sonication system such as that described in U.S. Patent No. 4,957,656, the residence time in an acoustic cavitation process makes it harder to control than in a true single-pass hydrodynamic cavitation system such as is described by the present invention.

:Microbubble suspensions produced by mechanical shear forces are used per se as contrast agents or formed into microspheres by further processing. For instance, PCT Publication No. WO 92/05806 describes the preparation of a rnicrobubble suspension, which they refer to as a "foam" 1 of a filmogenic protein which is prepared by whipping a protein solution containing viscosifiers into a coarse foam at a constant temperature below that which would denature the protein. The resultant foam is then sheared mechanically to form bubbles of a desired range which are stabilized by the presence of the viscosifiers. The bubbles may then be further processed into inicrospheres by heat denaturation or by the addition of cross-linkers to harden the protein film surrounding the bubbles.

European Patent Application Pub. No. 0 450 745 Al describes a process for making microspheres by forming an oil-in-water emulsion by mechanical shearing and simultaneously or subsequently adding a waterinsoluble polymer which deposits at the interface. The hydrophobic phase is then evaporated to form air or gas filled mnicrospheres.

Thus, the present invention also relates to an improved method for making microspheres from heat denaturable protein by subjecting a protein solution to mechanical shear forces. Such forces create a suspension of microbubbles that are simultaneously or subsequently encapsulated by a discrete shell. Because of the nature of cavitational heating, the denaturation of the protein is localized and forms the shell by depositing at the liquid-gas interface. This new method is easier to scale-up and leads to improved product yields as compared to the prior acoustic-based methods of making microspheres.

Disclosure of the Invention One aspect of the invention is a method of making encapsulated gas microspheres useful as an ultrasonic imaging agent comprising subjecting a mixture of an aqueous solution of a filmogenic protein and a pharmacologically acceptable water insoluble gas to ultrasonic or mechanical cavitation in the absence of oxygen.

Another aspect of the invention is a method of making encapsulated gas nicrospheres useful as an ultrasonic imaging agent comprising subjecting a mixture of an aqueous solution of a filmogenic protein and a pharmacologically acceptable insoluble gas to ultrasonic or mechanical cavitation in an apparatus that is closed to the atmosphere.

.Another aspect of the invention is an ultrasonic imaging agent composition comprising an aqueous suspension of microspheres of a gas encapsulated by a heat-insolubilized filmogenic protein wherein the S. 20 encapsulated gas is entirely a pharmacologically acceptable water insoluble gas.

Another aspect of the invention is an ultrasonic imaging agent composition comprising an aqueous suspension of inicrospheres of 25 "perfluoropropane gas encapsulated by a heat-insolubilized filmogenic 25 protein.

Another aspect of the invention is a method of making encapsulated gas microspheres useful as an ultrasonic imaging agent comprising: a) providing an aqueous solution of a heat-denaturable protein at a temperature necessary to achieve incipient denaturation temperature during subsequent mechanical emulsification; b) conmbining the solution with a gas; c) emulsifying the protein solution and gas mixture by mechanically shearing the mixture to form a suspension of gas microbubbles having a mean diameter in the range of about 0.1 to about 10 microns; and d) encapsulating the gas microbubbles to form microspheres by mechanically cavitating the suspension to cause the protein to become denatured and thereby deposited at the gas-solution interface.

Brief Description of the Drawings Fig.1 is an exploded schematic view of one type of mill (a Gaulin mill) that may be used in the mechanical cavitation process of the invention; Fig. 2 is an exploded schematic view of another type of mill (a Bematek mill) that may be used in the mechanical cavitation process of the invention; Fig. 3 is an exploded schematic view of still another type of mill (a Silverson mill) that may be used in the mechanical cavitation process of the invention; Fig. 4a shows the pressure resistance of air filled albumin microspheres. A microsphere suspension was placed in a syringe and pressurized at 40 psig. Particle distributions before and after pressurization are shown; Fig. 4b shows the pressure resistance of perfluoropropane-filled albumin microspheres. A microsphere suspension was placed in a syringe and pressurized at 40 psig. Particle distributions before and after pressurization are shown; Fig. 4c shows the pressure resistance of perfluoroethane-filled albumin 20 microspheres. A microsphere suspension was placed in a syringe and pressurized at 40 psig. Particle distributions before and after pressurization are shown.

Fig. 4d shows the pressure resistance of sulfur hexafluoride-filled albumin microspheres. A microsphere suspension was placed in a syringe 25 and pressurized at 40 psig. Particle distributions before and after pressurization are shown; Fig. 4e shows the pressure resistance of argon-filled albumin microspheres. A microsphere suspension was placed in a syringe and pressurized at 40 psig. Particle distributions before and after pressurization are shown.

Fig. 5 shows the pressure resistance of dilute suspensions of microspheres at 3.0 psig. A diluted suspension of microspheres were placed in a 1 cm cuvette and subjected to 3.0 psig at the time t= 30 seconds. Shown are data for perfluoroethane, perfluoropropane, sulfur hexafluoride and air microspheres; Fig. 6 shows pressure resistance of dilute suspension of argon microspheres at 3.0 psig. Diluted argon microspheres were placed in a 1 cm cuvette and subjected to 3.0 psig at time t=30 seconds.

Fig. 7 shows the effect of degassed buffer on microspheres.

Microspheres were added to increasing amounts of degassed buffer, with mixing, and the mixture brought to constant volume for determination of concentration. Data for air, perfluoropropane, perfluoroethane, and sulfur hexafluoride microspheres are plotted versus volume of degassed buffer; and Fig. 8 shows graphs of data described in Example 11, infra.

Modes for Carrying Out the Invention The novel microspheres of the present invention are formed in an aqueous suspension from the ultrasonic or mechanical cavitation of aqueous solutions of filmogenic proteins in the presence of an insoluble gas and in the substantial absence of oxygen, under anaerobic (closed system) conditions. The microspheres are echo reflective and of a size suitable for transpulmonary passage, with a mean diameter less than 10 microns and greater than 0.1 microns. The size distribution may be altered by fractionation into larger or smaller microsphere populations. The microspheres may or may not be concentrated by removal of excess aqueous 20 phase, or collected and resuspended in a second aqueous solution.

The gas used to make these novel microspheres need only be pharmacologically acceptable and insoluble in the aqueous media in which they are placed initially the medium in which they are made and, when used, in the blood). Solubility in water is a close approximation of solubility 25 in such media. The term "gas" refers to any compound which is a gas or capable of forming gas at the temperature at which imaging is being performed (typically normal physiological temperature). The gas may be composed of a single compound or a mixture of compounds. Appropriate gases would include, but are not limited to, fluorine-containing gases such as sulfur hexafluoride, perfluoroethane, perfluoropropane, perfluoromethane, and perfluorobutane. Solubility of a gas can be defined by determining the Bunsen Coefficient of the gas of interest. This value is the volume of gas which is absorbed by a unit volume of solvent. (see Wen, W-Y, Muccitelli, JA, J. Sol. Chem. 8:225-240 (1979)). Gas suitable for use in the present invention should have a Bunsen Coefficient in water at 25 0 C of less than 0.01 mL/niL of solution. Table 1 gives the Bunsen Coefficients of several gases.

Table 1 BUNSEN COEFFICIENTS OF GASES IN WATER (1 atmosphere, mL/mL) Gas 5 0 C Carbon dioxide 1.383 0.824 Argon 0.047 0.033 Oxygen 0.043 0.031 Nitrogen 0.021 0.016 Sulfur Hexafluoride 0.008 0.0054 Perfluoromethane 0.0082 0.00504 Perfluoroethane 0.0027 0.00138 Perfluoropropane 0.0016 N/A Perfluorobutane 0.0007 N/A Another characteristic of the gas contained in the microspheres is that "the diffusivity of the gas is less than 4 x 10- 5 cm 2 /sec at 25°C in water. It should be noted, however, that the diffusivity constant varies in different solvents and at different temperatures, but for purposes of selecting a gas, the gas should meet this criterion.

Pharmacologically acceptable refers to the property that the selected gas must be biocoinpatible and have minimal toxicity.

S: Perfluoropropane is preferred because it provides an insoluble gas that will not condense at the temperatures of manufacture and use, has no isomeric forms, produces microspheres that exhibit excellent pressure resistance, and is pharmacologically acceptable.

The gas microbubble is encapsulated by a filmogenic protein shell.

The term filmogenic refers to the ability of the protein to form a shell around the entrapped gas, with hydrophilic groups oriented externally and hydrophobic groups oriented internally, upon protein insolubilization (caused by heat denaturation). The protein will necessarily have both hydrophilic and hydrophobic amino acids. Suitable proteins will include naturally occurring proteins such as albumin, gamma-globulin (human), apotransferrin (human), b-lactoglobulin, and urease. Although naturally occurring proteins are preferred synthetic proteins (homopolymeric or heteropolymeric) which exhibit tertiary structure and are susceptible to heat denaturation may be used. Particularly well suited for the present invention is albumin, and more particularly, human albumin. The protein is present in the solution at a concentration in the range of about 0.1 to w/v, preferably about 1 to 5% w/v, and most preferably about 1% w/v.

Proteins suitable for the present invention, or the resulting microspheres, may be chemically modified for the purpose of organ targeting or quenching immunogenic activity modification with polyethylene glycol). However, the present invention does not involve addition of chemical crosslinking agents, or additional modifications to the proteins for the purpose of forming the microspheres.

The microspheres of the present invention are formed by insolubilizing portions of a protein in solution as a result of cavitation in the presence of an insoluble gas, without the presence of oxygen in a closed system in which air contamination is avoided.) Such protein insolubilization is characterized primarily by local protein denaturation and orientation around 20 the gas core, the latter of which may be enhanced in the presence of the •insoluble gas.

The system used to heat-insolubilize the protein formation of the microspheres of the present invention must be anaerobic, closed to the 'atmosphere, and is referred to as a "closed system". In comparison, an "open 25 system" is one which is open to the atmosphere. The gas entrapped in microspheres made in such a closed system will necessarily contain only the insoluble gas used for formation. Contamination by atmospheric gases can be monitored using an 02 electrode to measure the presence of 02 in the system effluent. In the present invention, microspheres are produced which initially contain only the gas used for formation. However, when the gas content is determined experimentally, there is a certain amount of unavoidable contamination by the atmospheric gases during the experimental procedure, thus causing the amount of resultant gas measured to be less than 100%. Accordingly, measurements of gas greater than are representative of microspheres whose initial gas content was entirely insoluble gas.

After formation of the microspheres, exposure to the atmosphere should be avoided during packaging. For example, microspheres should be sealed in vials or other air-tight containers within 5 to 30 seconds from the time they exit from the closed system. Additionally, any head space in the vials should be removed and replaced with the gas used in formation during packaging.

Filled with an insoluble gas, these protein microspheres exhibit remarkable stability, surviving exposure of 40 psig >2000 mm Hg) at a concentration of about 1.0 x 109 microspheres per mL. The microspheres also exhibit elasticity in a dilute suspension, exhibiting compression under pressure of 3-10 psig, and returning to the original volume upon the release of pressure. Additional chemical cross-linking would be disadvantageous because the resultant microspheres would have too rigid a structure to exhibit enhanced pressure stability.

The microspheres of the present invention, unlike free microbubbles, are resistant to coalescence and diffusion driven expansion. Microspheres containing insoluble gas incubated in air or oxygen saturated solution, at various temperatures, do not increase in mean diameter or increase in total S. volume. The protein shell, although elastic when subjected to pressure, is S 20 strong enough to resist expansion or tearing by gas diffusion or exchange.

The presence of the protein shell prevents coalescence, and maintains the gas in small, individual bubbles for up to several months, similar to air-filled protein microspheres. The inability of insoluble gas-filled microspheres to measurably expand by exchange with solvated atmospheric gases is a novel 25 and key property for the use of this material as an ultrasound agent.

Insoluble gas-entrapped protein microspheres exhibit resistance to collapse upon exposure to degassed aqueous solutions. Unlike free microbubbles or encapsulated microspheres filled with air, insoluble gasfilled microspheres may be added to vacuum degassed water and maintain integrity at high dilution. Air-filled material collapses in blood due to the efflux of the oxygen component of the gas phase. The ability of microspheres filled with an insoluble gas to resist collapse in a partially degassed or pressurized environment increases dramatically the duration of ultrasound contrast in vivo.

The mnicrospheres of the present invention can be produced by ultrasound or mechanical cavitation. A process of ultrasound production of air-filled in-icrospheres has been described by Cerny (USP 4,957,656).

Mechanical cavitation is a preferred process for making the novel insoluble gas-filled microspheres of this invention. It may also be used to make air-filled or soluble gas N 2

H

2 argon) -filled microspheres.

Iii the novel mechanical cavitation procedure of the present invention, the aqueous solution of heat denaturable protein is provided at a temperature necessary to achieve incipient denaturation temperature during the subsequent mechanical emulsification of the solution. The denaturation temperature of the protein in solution will normally be in the range of 50 to 100 0 C. It can be obtained from tables of thermal protein denaturation in the literature, or experimentally by any known method. For example, to determine the denaturation temperature experimentally, a protein solution can be heated in a water bath while stirring. The denaturation temperature is the temperature at which insoluble material is first observed. Note that the :denaturation tenmperature is affected by the nature, purity and source of the protein, the concentration of protein in the solution, the pH, buffer, ionic see* 20strength, the presence of stabilizers and the presence of chemical denaturants or detergents. Therefore, it is necessary to determine the denaturation temperature of the protein in the environment in which it will be employed to make mnicrospheres. If desired, additives such as detergents 0 g or polar solvents can be employed to change the temperature at which 25 denaturation takes place.

Table 2 gives the denaturation temperatures of several naturally occurrinig proteins which were predetermined experimentally as described above: Table 2

C

'C Protein Concentration pH Solvent Tdonaturation Human Serum 50 mng/mIL 6.9 75 0

C

Albumin, USP NaCi.

Swiss Red Cross 4miM (Bern. Sodium Switzerland) Caprylate 4 miM Tryptoph anate Human Serum 10 miglmL 6.9 78 0

C

Albumin, USP NaCi, Swiss Red Cross 1m1M (Bern. Sodium Switzerland) Caprylate 1 m1M Tryptoph anate j-Lactoglobulin, 25 mig/nIL 7.6 USP 90 0

C

Sigma (St. Louis. Water MO) cu-Globin, 25 mg/miL 5.0 USP 90 0

C

Sigma (St. Louis. Water

MO)

Lysozyme 100 mg/mI1 7.5 5 niM 31 0 C as Sigma (St. Louis. TRIS* determined MO) 2miM immediately DTT* after addition of DTT Human Gamima 40 mg/i- 5.0 10 m1M 66 0

C

Globulin, acid MES** pH method, pH Sigma (St. Louis.

MO) Protein Concentration pH Solvent Titonauration Human Gamma 40 mg/niL 9.8 10 Mm 69 0

C

Globulin,

TRIS,

alkaline pH pH 9.8 method Sigma (St. Louis.

MO)

apo-Transferrin, 20 mg/mL 7.5 10 m1M 71 0

C

Sigma (St. Louis.

TRIS*

MO) II TRIS 2-amino-2-(hydroxymethyl)-1,3-propanediol MES 2-(N-morpholino)ethanesulfonic acid DTT dithiothreitol Each apparatus employed to shear the protein solution/gas mixture will cause a certain amount of additional heating of the protein solution due to the mechanical shear forces exerted on the solution. That heat must be sufficient to cause localized denaturation of the protein at the gas-liquid S":interface. It is thus important to determine the amount of temperature increase caused by the apparatus so that the temperature at which the protein solution is introduced into the apparatus can be adjusted to achieve such local thermal denaturation. Specifically, the bulk temperature of the liquid in the apparatus must coincide with the incipient denaturation temperature immediately prior to cavitation. The cavitation event generates the additional heat necessary to locally denature the protein. Incipient denaturation temperature is defined as the temperature at which the protein is on the verge of denaturation, but the solution does not contain any denatured protein. This temperature is just below, typically 1 to 5oC below, the denaturation temperature. If necessary, the starting protein solution may be preheated prior to being introduced into the apparatus to a temperature that allows the incipient denaturation temperature to be reached.

Once the proper starting temperature of the protein solution has been achieved, the solution is combined with a suitable gas, for example by introducing the gas into the protein solution prior to or during the emulsification step at a volume to volume ratio in the range of about 5% to 200% gas: liquid, preferably about 20% to 100%. The proper gas: liquid ratio will depend on the geometry of the apparatus, the physical characteristics of the gas (solubility, density, molecular weight, etc.), and can be adjusted to optimize output.

After the gas and protein solution are combined, the mixture is emulsified and subjected to cavitation under conditions that produce microspheres. This is accomplished using an apparatus in which mechanical shearing and hydrodynamic cavitation can be produced, such as high speed mixers, mills, fluidizers and the like. A preferred apparatus is a colloid mill which is defined as "a machine consisting of a high-speed rotor and a stator, dispersion or emulsification being effected by the opposing faces." Advanced Filtration and Separation Technology, p.108-110. Examples of specific milling apparatuses which can be used are as follows: Model #2 1/2 Bematek, Beverly, MA Model W250V- Greerco, Hudson, NH Model 2F APV Gaulin, Everett, MA Model L4R Silverson, Chesham, UK Model Polytron PT3000- Kinematica, Littaw, Switzerland When used to make insoluble gas-filled microspheres, the colloid mills S 20 should be closed to the atmosphere so as to avoid introducing air into the mixture.

Figures 1-3 provide further details of several types of mills that may be used in the mechanical cavitation process.

Fig. 1 depicts the essential elements of a Gaulin mill. These are: a rotating shaft (10) operably connected to a motor (not shown); a disc rotor (12) affixed to the end of the shaft and a stator Stator (11) has a central bore opening (18) and a counterbore (16) into which the rotor is received. In this mill the protein solution and gas are fed through the mill via a "tee" The protein solution/gas mixture is emulsified and cavitates 30 between the surfaces of the rotor and stator. The "gap" in this mill is the space between radial surface (17) of the stator counterbore and the circumferential radial surface of the rotor. The temperature of the microsphere product will be taken with a thermocouple, not shown) as the mixture exits past the stator (11).

Fig. 2 shows the essential elements of a Bematek mill. This mill is similar in structure and function to the Gaulin mill of Fig. 1 the main difference being the configurations of the rotor and the stator counterbore. It includes a rotary shaft (20) that carries a frustroconical rotor (21) that has a threaded leading end (22) and a stator (23) that has a central cylindrical opening (25) and a frustroconical counter-bore (24) that is adapted to receive the rotor. The protein solution/gas mixture is fed into this mill via opening The gas and solution are ,nixed as they pass by the threads on the shaft (22) and the mixture is emulsified and subjected to cavitation as it passes through the gap of the mill. The gap is defined by the space between the conical surfaces of the rotor and stator.

Fig. 3 illustrates a Silverson mill. The structure of this mill is quite different from those of the mills of Figs 1 and 2. The depicted Silverson mill has a rotating shaft (30) that carries a paddle blade rotor The rotor is received in a cup-shaped perforated screen stator The stator is mounted on a housing (33) fitted with an inlet fitting The inlet fitting (34) extends up into the housing (33) opening at the bottom center of the perforated screen stator The housing has a central opening (not shown) that conmmunicates with the inlet fitting and with an opening (also not shown) in the bottom of the stator. In this mill the solution/gas is fed via the inlet fitting into the bottom of the stator and is emulsified and cavitated between the flat faces (35) of the paddle rotor and the inner cylindrical surface of the stator. The "gap" of this mill can be defined as the space between the rotor (31) and stator but the effect of the gap size on the process is influenced by the size of the perforations (36) of the stator.

:After passing through the mill the product may be cooled, typically to 10 20 0 C, and defoamed by settling or by adding biocompatible defoaming agents that do not adversely affect the microspheres.

Passing the mixture through such a mill or equivalent device emulsifies and cavitates the mixture to form microspheres in the range of about 0.1 to 10 microns (mean diameter). Microsphere size may be 30 determined by a suitable particle counter, for example a Coulter Multisizer II (Courter Electronics, Hialeah, Fl).

When using a mill such as those described in Figs. 1-3, the rotor speed, gap size and gas: liquid ratio are the principal process parameters which affect the characteristics (mean size, size distribution, and concentration of mnicrospheres) of the microsphere product. Those parameters are adjusted empirically to provide a product having the desired characteristics. For any given product, its characteristics are defined clinically. For instance, putative specifications for perfinoropropane microspheres used for myocardial perfusions are: mean size, 4 microns; size distribution, 90% under 10 microns; concentration, 7 x 18to 2 miicrospheres/miL.

The invention is further illustrated by the following examples. These examples are not intended to limit the invention in any manner.

EXAMPLE 1 Mechanical Cavitation Process Temperature Monitoring and Control for Human Serum Albumin As described above, the protein solution is pre-heated before processing so that the process temperature can reach and maintain the incipient denaturation temperature.

A typical method of practice is as follows: A Model. 2 1/2" Bemnatek Colloid Mill (Fig. 2; Bemnatek Systems, Beverly MA), was piped so that the inlet port was connected to a heat exchanger. Gas impermeable tubing was used to make the soft connections between the heat exchanger hose barbs.

The outlet port from the process head was connected to a stainless steel post-process chiller.

hetSolution temperature was monitored at three sites (Ti, T2 and T3).

The Ti thermocouple was mounted in a Swagelok "Tee" between the pre-heat hetexchanger and the mill head to measure the feed temperature of the protein solution. A second "Tee" for introducing gas was also placed at the feed port. The T2 thermocouple was placed inside the exit from the process head, approximately 1 cm from the rotor and 2 cm from the shaft so that the temperature of the process could be accurately measured. In this way, the two temperatures can be measured independently, the feed temperature (Ti) and the process temperature, and compared to determine the amount of heating of the solution during processing.

For this example, U.S.P. albumin was diluted with normal saline to make up a solution. The denaturation temperature was determined expe rime ntally, as described, to be 78 0 C. It was fed into the mill at 200 nuL/min following degassing along with perfluoropropane at 100 niL/mmn (50% Differences between Ti and T2 of 100 to 15 0 C were noted. In order to obtain a process temperature of 77 0 C (1 0 C below denaturation temperature), the feed temperature was adjusted to a range of 620 to 67 0

C.

Since the amount of heat generated will vary with different milling parameters, it is necessary to determine the difference between T1 and T2 with each change in milling parameters, (choice of mill, mill settings, flow rate, gas:liquid ratio, etc.) in order to target the process temperature to avoid bulk denaturation of the protein while successfully encapsulating the gas microbubbles with a thin shell of denatured protein.

The chiller-out temperature (T3) was also monitored, and for best results was targeted at 20 0

C.

EXAMPLE 2 Mechanical Cavitation Method of Making Microspheres Containing Different Gases Microspheres containing various gases were produced as follows: human albumin solution (USP) was deaerated under continuous vacuum for two hours. The vacuum was released by filling the evacuated vessel with the gas of interest. Insoluble gases utilized include sulfur hexafluoride, perfluoroethane, and perfluoropropane. Microspheres containing more soluble gases, air, nitrogen, oxygen and argon, were also produced. The use of argon was representative of a high molecular weight, but relatively soluble, gas. The albumin solution was adjusted to 68°C via an in-line heat exchanger and pumped at 100 mL/min into a 2 1/2" colloid mill (Greerco, Hudson, NH, model W250V or AF Gaulin, Everett, MA, model 2F). The specific gas, at room temperature, was added to the liquid feed just upstream of the inlet port at a flow rate of 120-220 mL/min. The gap between the rotor and the stator was adjusted to 2/1000th inch (0.005 cm) and the albumin solution was milled continuously at about 7000 rpm at a process temperature of 73 C.

The dense white solution of microspheres thus formed was 30 immediately chilled to a temperature of 10°C by a heat exchanger, and collected in glass vials. The vials were immediately sealed. The material was characterized with regard to concentration and size distribution using a Coulter Counter. The results are shown in Table 3 below.

Table 3

C

C

C

C

C.

eq

C

C

C

C C Concentration Mean Size (pspheres/mL) (microns) Perfluoropropane 8.3 x 108 3.8 Perfluoroethane 10.6 x 108 Sulfur hexafluoride 8.4 x 108 3.9 Air 9.2 x 10 7 3.4 Nitrogen 5.4 x 10 7 Oxygen 6.1 x 107 3.9 Argon 4.1 x 107 EXAMPLE 3 Effect of Rotor Speed and Gap Size A 1% albumin solution was combined (200 mL/min) with perfluoropropane (100 mL/min) at a 50% gas to liquid ratio.

Microspheres were prepared according to the procedure described in 10 Example 1 using varying rotor speeds and gap sizes. The data obtained are shown in Table 4.

Table 4 Rotor Tip Speed Gap (cm) Concentration Mean Size (fl/min) (pspheres/mL) (microns) 3500 0.01 0.76 x 108 13.4 4300 0.01 2.43 x 108 9.6 4800 0.01 9.38 x 108 3.8 9700 0.01 20.96 x 108 4.3 5200 0.02 12.87 x 108 7000 0.02 12.50 x 108 3.4 8700 0.02 14.42 x 108 9600 0.02 15.22 x 108 2.9 These results show that concentration increases and mean size decreases with increasing rotor speed while increasing gap size decreases concentration.

EXAMPLE 4 Effect of Gas to Liquid Ratio A 0.5% Albumin solution (100 mL/min) was combined with perfluoropropane at 20, 50, 70 or 100 mL/min (20, 50, 70 or 100% gas to liquid v/v) using a Gaulin mill with an approximate gap of 0.012 and a rotor tip speed of 9950 ft/min. The data obtained are shown in Table Table Gas/Liquid Gap Concentration Mean Size (cm) (pspheres/mL) (microns) 0.03 6.54 x 108 3.6 0.03 7.51 x 108 4.3 0.03 8.76 x 108 100 0.03 8.66 x 108 5.9 These results show that both concentration and mean size increase with an increase in gas: liquid ratio.

15 EXAMPLE Method of Making Insoluble Gas-Filled Microspheres by Sonic cavitation Air, sulfur hexafluoride and perfluoroethane microspheres were prepared by both batch and continuous ultrasound cavitation processes. A 20 solution of human albumin, USP, was degassed under vacuum and stored under the specific gas. The continuous sonication process was performed as described by Cerny (USP 4,957,656), substituting the insoluble gases for air. The batch process was performed utilizing a 3/4" liquid e processing horn (Sonics and Materials, Danbury CT). Gas was passed through the horn and into the albumin such that during the entire process air was excluded. The albumin was warmed to 73'C and sonicated for 5 sec at KHz at 60 microns double amplitude, using a Branson piezoelectric converter and power source (Branson Ultrasonics, Danbury CT). The product was immediately transferred to a glass vial and sealed under gas.

The product consisted of a thick, milky suspension of microspheres at concentrations of 1.4 x 108 to 1.0 x 10" microspheres/mL with a mean size of to 3.3 microns.

EXAMPLE 6 Microscopic Examination of Microspheres Albumin microspheres containing various gases were prepared as described in Example 2 or 5. Microscopic examination of the products revealed a monodisperse suspension of spherical microspheres. The microspheres were collapsed by application of high pressure in a syringe' until the suspension clarified. In all cases, microscopic reexamination revealed the presence of hyaline, membranous shells from the collapsed microspheres.

EXAMPLE 7 Pressure Resistance of Microspheres Albumin microspheres containing various gases were prepared as described in Example 2. Ten mL of each suspension was placed in a 10 mL glass gas-tight syringe (Hamilton, Reno NV) fitted with a pressure gauge. All headspace was removed and the apparatus was sealed. A constant pressure of 40 psig was applied for 3 min. A Coulter Counter was then used to 20 measure the sample particle concentration and distribution. Comparisons of the data (Figures 4a-4e) before and after pressurization demonstrated a relative resistance of the insoluble gas microspheres to 40 psig.

EXAMPLE 8 Pressure Resistance of Dilute Suspensions of Microspheres Microspheres containing various gases were prepared as described in Example 2. Each sample of microspheres was diluted to an equal volume of encapsulated gas per mL of phosphate-buffered saline (0.15M), about a 1:60 dilution. The diluted suspension was subjected to instant static pressures of 30 0.5 psig to 7.5 psig in a sealed vessel with adequate head space. Figure shows the effect of pressure on microsphere concentration. Microspheres containing the insoluble gases perfluoropropane, perfluoroethane and sulfur hexafluoride are much more pressure-resistant than air or high molecular weight argon-filled microspheres of the same concentration and size distributions (Figure Physiological pressures in the bloodstream range from a peripheral venous pressure of 1.5 psig to 2.5 psig in the myocardial wall.

EXAMPLE 9 Effect of Degassed Buffer on Microspheres Albumin microspheres containing various gases were prepared as described in Example 2. Phosphate-buffered saline (PBS) was degassed by boiling just before use. 0.05 mL to 1.5 mL aliquots of the hot buffer were placed in 13 x 100 test tubes and allowed to cool 1 min to room temperature in a water bath. A constant volume of microspheres was added to each tube.

After mixing, the final volume was brought to 3.0 mL with PBS and the microsphere concentration was determined. Figure 7 shows that improved survival in degassed solutions is obtained for the microspheres containing the insoluble gases perfluoropropane, perfluoroethane and sulfur hexafluoride.

Microspheres containing air, sulfur hexafluoride or perfluoroethane were diluted into whole blood. Air-filled microspheres exhibited collapse.

The insoluble gas-filled microspheres were shown to survive dilution in fresh whole blood.

EXAMPLE 20 Elasticity Microspheres prepared from various gases were prepared as described in Example 2. Microspheres were diluted into phosphate-buffered saline, as i described in Example 8, and placed in a clear cell positioned on the stage of a microscope. The cell was connected to a nitrogen source that allowed for observing the effects of rapid application and release of physiological pressures on the microspheres.

The application of 1.5 psig or greater to the soluble gas containing s microspheres resulted in observing the complete loss of spherical bodies.

The microspheres did not reform upon the release of the pressure, indicating 30 irreversible destruction. The application of less than 1.5 psig resulted in deformation and wrinkling of the shell with incomplete loss of microspheres.

The spherical appearance or population could not be restored upon release of the applied pressure.

The application of pressure up to several psig to a suspension of microspheres containing the insoluble perfluorocarbon gases resulted in a reduction of the diameter of the microspheres. The diameter of the microspheres returned to the original dimensions upon the release of the pressure.

Sulfur hexafluoride microspheres also exhibited enhanced elasticity under applied physiological pressure relative to air-filled microspheres, but less elasticity relative to the perfluorocarbon microspheres.

These observations indicate that the microspheres containing insoluble gases were not only resistant to pressure, but recovered after pressure was released. This is indicative of an elastic protein shell.

EXAMPLE 11 Comparison of Microspheres Made in an Open System and in a Closed System PROCESS METHODS A) Manual Sonication: Open System (Equivalent to EPA 554,213 One-Step Method) The method described in U.S. Patent No. 4,844,882 and European Patent Application 554,213 was used to prepare microspheres as follows: A 20 cc syringe barrel was fitted with a T-type thermocouple inserted through the tip and mounted onto a support stand. The syringe was filled to *the 16 cc mark with Swiss Red Cross 5% human serum albumin. Gas 20 (perfluoropropane (C 3 or F 8 or sulfur hexafluoride was introduced into the top of the syringe barrel and flowed over the surface of the liquid. A sonicating horn was lowered to the 10 cc mark, below the surface of the solution, and operated at 50% power until the temperature of the solution rose to 72.8 73 0 C; approximately 1 minute. The horn was immediately withdrawn to the meniscus 1 mm and the power level increased to Sonication continued for 5 seconds, with an additional temperature increase of 1.2 2 0 C. The product was poured into a glass vial to capacity and sealed.

S: B) Continuous Sonication: Closed System The method described in U.S. Patent No. 4,957,656 was used to S: 30 prepare perfluoropropane and sulfur hexafluoride microspheres as follows: Human serum albumin was diluted to a 1% w/v solution with sterile saline. The solution was heated to incipient denaturation, approximately 76°C. The system was closed to the external atmosphere and perfluoropropane or sulfur hexafluoride gas was introduced into the liquid flow in place of air. The product was made continuously by flowing the gas/albumin mixture past the sonicator horn at approximately 100 ml liquid/min. The product was chilled upon exit from the sonication chamber by passage through a heat exchanger and collected as a bulk liquid suspension of microspheres. Handling and storage conditions were similar to that given for manually produced microspheres.

C) Mechanical Cavitation: Closed System Albumin microspheres containing perfluoropropane or sulfur hexafluoride gas were also produced in a closed system by milling a mixture of 1% human serum albumin and gas, similar to that described in Example 2.

Albumin solution, heated to a temperature sufficient to allow microsphere formation by the mechanical cavitation of a given mill, was mixed 1:1 v) gas and introduced into a colloid mill. The liquid flow rate was dependent upon the capacity or size of the mill, typically 100 to 500 ml/min. A Silverson L4R mill and a Bematek 3" production colloid mill were used for this evaluation. The outflow from the mill was cooled by passage through a heat exchange system and the resulting albumin microsphere suspension was collected in bulk. The product was filled into glass vials, similar to the other processes.

ANALYTICAL METHODS A) Population Dynamics 20 Population dynamics were evaluated with a Coulter Multisizer II using a 50 micron aperture. Albumin microspheres prepared as described in PROCESS METHODS were diluted 1:10,000 into Isoton and a 500 pl sample was analyzed. Concentration, mean size, and encapsulated gas volume per ml of the original microsphere suspension were obtained.

B) Gas Content The percentage of perfluoropropane entrapped in duplicate lots of microspheres prepared as described in PROCESS METHODS was determined by gas chromatography on a Hewlett Packard 5890. A sample of the microsphere suspension was taken in a gas tight syringe. The gas was 30 released from the microspheres using an anti-foam agent in ethanol and the entrapped gas was detected by thermal conductivity.

C) Pressure Resistance Pressure resistance of albumin microspheres was evaluated by a method similar to that reported by Sintetica in European Patent Application 554,213. Microspheres were diluted in aerated phosphate buffered saline, to approximately 1 absorbance unit at 600 nm, in a 3 ml pressure cuvette. The neck was attached to a pressure source and the cuvette placed in a recording spectrophotometer. The pressure in the cuvette was increased linearly from 0 to 5 or 10 psig over 150 seconds, at which time the pressure was released.

The pressure ramp was created by a proportioning solenoid valve (Honeywell) and a pressure transducer (Omega) that were placed between a psi pressure source (N 2 tank) and a 5 liter stainless steel reservoir. The cuvette was connected to the steel reservoir through a digital pressure gauge.

A PC-type computer equipped with an analog to digital converter and a digital to analog converter board (National Instruments) controlled the opening of the valve and read the pressure transducer. The reservoir, and cuvette, was pressurized at a selected rate until the desired pressure was achieved. The optical density of the microsphere suspension was monitored as a function of time and pressure. The data was corrected for the natural flotation rate of microspheres in the cuvetee.

RESULTS

A) Population Dynamics Albumin microspheres produced by the methods of manual sonication, continuous sonication and mechanical cavitation were analyzed for concentration, mean size, encapsulated gas volume and size distribution 20 within 24 hours after manufacture. All measurements were performed in duplicate, as a minimum, and are presented as the average. The results of these measurements are given in Table 6.

Table 6 9 *999 9. 9 9. 9.

9 9 Gas Method Cone. Mean Size Vol (10"/ml) (ml/ml)

SF

6 Manual Sonication 8.7 3.2 0.046

SF

6 Continuous Sonication 12.7 2.7 0.034

SF

6 Mechanical 10.0 3.8 0.054 Cavitation

C

3

F

8 Manual Sonication 13.4 2.8 0.033

C

3

F

8 Manual Sonication 17.7 2.8 0.056

C

3

F

8 Continuous Sonication 10.1 3.0 0.050

C

3 F, Continuous 6.7 4.3 0.127 Sonication Gas Method Cone. Mean Size Vol (108/ml) (ml/ml)

C

3 F, Mechanical 31.0 3.0 0.23 Cavitation (Bematek Mill)

C

3

F

8 Mechanical 6.9 5.0 0.34 Cavitation (Silverson Mill) Microspheres produced by all methods were stable for the duration of this study, at least several weeks at 4 0

C.

B) Gas Content Analyses of the composition of entrapped perfluoropropane gas in duplicate lots of microspheres are given in Table 7.

Table 7 M ethod C 3F, Manual Sonication 70.0 Continuous Sonication 89.5 Mechanical Cavitation 95.5 Average results of duplicate lots.

These results demonstrate that microspheres made in the open system using manual sonication encapsulate much less of the gas used to form the 15 microspheres than those made in the closed systems (continuous sonication and mechanical cavitation.) The microspheres made in the closed system were made in the absence of oxygen, as determined using an oxygen electrode. Microspheres made by all three methods were subjected to the same amount of exposure to the atmosphere during handling and sampling (which accounts for less than 100% perfluoropropane gas being measured in microspheres made using the two closed system procedures), thus there was oxygen (and other atmospheric gases) present during formation in the open system which diminished the efficiency of gas encapsulation.

.9 9 99..

9 9 9* 9 9q 9.

.9 9 9*q 9 9.

*9 p 27 C) Pressure Resistance A suspension of gas-filled microspheres will decrease in optical density with increasing pressure due to a decrease in size and associated change in surface area. Shrinkage is due to two factors; reversible compression according to the gas laws, and irreversible loss of the gas core to the surrounding liquid due to increased solubility according to Henry's law.

Upon the release of an applied pressure, only that fraction of the volume loss due to compression is recovered, and which can be observed by an increase in optical density. The loss of entrapped gas to the surrounding solution does not reenter the microspheres upon depressurization, but is lost to the head space above the solution.

Fig. 8 shows the result of imposing a linear pressure gradient up to psi on 1 OD suspensions of albumin microspheres prepared with perfluoropropane gas by the manual sonication (open system) method as well as the continuous sonication and mechanical cavitation (closed system) methods. Both closed system methods yielded microspheres that exhibited compression with increasing pressure, with a total recovery of volume upon release of the pressure at the end of the gradient. Loss of entrapped gas to the surrounding solution was not observed. Albumin microspheres prepared 20 in the open system (manual sonication method) exhibited greater compression with applied pressure and only a partial recovery of volume upon release of pressure due to the irreversible loss of the gas core, resulting in a 40% destruction of microspheres.

a* o

Claims (11)

1. A method of making encapsulated gas microsphers useful as an ultrasonic imaging agent comprising: a) providing an aqueous solution of a heat-denaturable protein at a temperature necessary to achieve incipient denaturation temperature during subsequent mechanical emulsification; b) combining the solution with a gas; c) emulsifying the protein solution and gas mixture by mechanically shearing the mixture to form a suspension of gas microbubbles having a mean diameter in the range of about 0.1 to about 10 microns; and d) encapsulating the gas microbubbles to form microspheres by mechanically cavitating the suspension to cause the protein to become denatured and thereby deposited at the gas-solution interface.

2. The method of claim 1 wherein said temperature is achieved by heating the solution.

3. The method of claim 1 wherein said temperature is achieved by including additives in the solution that alter the denaturation temperature of the protein.

4. The method of claim 1 wherein the protein is a naturally occurring protein. The method of claim 4 wherein the protein is human serum albumin.

6. The method of claim 1 wherein the protein is synthetic protein. S7. The method of claim 1 wherein the concentration of the protein in the solution is about 0.1 to 10% w/v.

8. The method of claim 1 wherein the concentration of the protein in the solution is about I to 5% w/v.

9. The method of claim 1 wherein the concentration of the protein in the solution is about 1% w/v. The method of claim 1 wherein the gas is insoluble. 30 11. The method of claim 10 wherein the insoluble gas is sulfur hexafluoride, perfluoromethane, perfluoroethane, perfluoropropane, or perfluorobutane.

12. The method of claim 1 wherein the gas is air.

13. The method of claim 1 wherein the ratio of gas to protein solution is 5% to 200%v/v. 29

14. The mnethod of claim 1 wherein the ratio of gas to protein solution is to 100% v/v. the mnethod of claimi 1 wherein steps and are effected by passing the Mixture through a mill.

16. The method of claim 1 whereini the incipient denaturaton~ temperature is about 1 o to 5 0 C below the denaturatioii temperature of the protein. DATED this 5th day of May 1998 MOLECULA~R BIOSYSTEMS, INC Patent Attorneys for the Applicant: F. B. RICE& CO. se