AU721881B2

AU721881B2 - Ketone derivatives and medicinal use thereof

Info

Publication number: AU721881B2
Application number: AU20436/97A
Authority: AU
Inventors: Yasumoto Adachi; Hideki Kawai; Tsuyoshi Matsumoto; Youhei Miyamoto; Kiyoshi Okano; Yuji Sugawara
Original assignee: Toray Industries Inc
Current assignee: Toray Industries Inc
Priority date: 1996-03-27
Filing date: 1997-03-27
Publication date: 2000-07-13
Anticipated expiration: 2017-03-27
Also published as: AU2043697A

Description

1

DESCRIPTION

KETONE DERIVATIVES AND MEDICAL APPLICATION THEREOF Technical Field The present invention relates to ketone derivatives and an agent having the ketone derivatives or pharmaceutically acceptable salts thereof as an active ingredient, and in particular, relates to a hematopoietic agent.

Background Art Ketone derivatives include lactones and lactams. For example, carolic acid and carolynic acid are known as natural lactones and compounds disclosed in J. Chem. Soc.

Perkin Trans. I, 14, 1485-1491 (1976) and Synth. Comm., 22(6), 809-816 (1992) are known as non-naturally derived compounds. In addition, compounds disclosed in J. Org.

Chem., 40(13), 1927 (1975), Tetrahedron Lett., 33, 2821 (1975), Chem. Pharm. Bull., 32, .3724 (1984), Tetrahedron Lett., 163 (1973), and the like are known as compounds having the following general formula wherein R 4 is an ester group: OR9

R,

(CH

2 )n R4 S(r) 2 Lactams of the general formula wherein X is nitrogen are disclosed in Japanese Patent Laid-Open Nos.

2-279691, 4-49289, 2-48591, and 1-313488, Chem. Pharm.

Bull., 32(10), 4197-4204 (1984), Pharmazie, 43(7), 473-474 (1988), Monatsh. Chem., 123(1-2), 93-98 (1992), J. Inorg.

Biochem., 24(3), 167-181 (1985), J. Am. Chem. Soc., 107(18), 5219-5224 (1985), J. Org. Chem., 50 1344- 1346 (1985), and Chem. Rev., 95, 1981-2001 (1995).

Applications of lactones include, for example, a compound disclosed in Japanese Patent Laid-Open No.5-43568 and EP 0508690, which is known as an anti-inflammatory agent inhibiting phospholipase A2, a compound disclosed in Archive des Pharmazie (Weinhelm, Ger.) (1983), 316(2), 115-120, which is known as an anticoagulant, and a compound disclosed in Journal of Antibiotics (1994), 47(2), 143-7, which is known as an anti-AIDS agent S: inhibiting HIV-protease.

Applications of lactams include, for example, a compound disclosed in Chem. Pharm. Bull., 32(10), 4197- 4204 (1984), which is known as an agent having antibacterial activity, and a compound disclosed in J.

Antibiot., 33(2), 173-181 (1980), which is known as an anaerobic antibiotic. However, no ketone derivative has been knowntohave hematopoietic activity.

been known to have hematopoietic activity.

Advantageously, the present invention provides P:\OPERMIPDM\20436-97.074 .1513/00 -3ketone derivatives, medical agents which have such ketone derivatives or pharmaceutically acceptable salts thereof as an active ingredient in a haematopoietic agent used for preventing or treating cytopenia caused by cancer chemotherapy, radiation therapy, bone-marrow transplantation, drug therapy, immunological disorders, anaemia, or the like.

Disclosure of the Invention The present invention relates to: a method for increasing blood cells, in a patient in need thereof, comprising the administration of an effective amount of a ketone derivative of the following general formula or a pharmacologically acceptable salt thereof

OR

2 A

R,

A

X^

O

25 [In the above general formula R 1 is a hydrogen atom, a halogen atom, a hydrocarbon group having 1 to 19 carbon atoms (in which the hydrocarbon group may have one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a 30 hydroxy group, a nitro group, a methoxy group, an e e S- 4 ethoxy group, a carboxyl group, a carboethoxy group, a carbomethoxy group, and a cyano group),

-CO(CH

2 )qQ (wherein q is an integer of from 0 to and Q is a hydrogen atom, a hydrocarbon group having 1 to 6 carbon atoms (in which the hydrocarbon group may have one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a carboxyl group, a carboethoxy group, a carbomethoxy group, a cyano group, a trifluoromethyl group, a methylthio group, a phenylthio group, and a t-butyl group), a heterocyclic ring having 1 to 9 carbon atoms (in which the heterocyclic ring may have one or more substituents selected from the group essentially consisting of.a chlorine atom, a bromine atom, a fluorine atom, a.hydroxy group, a nitro group, a methoxy group, and an ethoxy group), a hydroxy group, a thiol group, a thioether group having 1 to 6 carbon atoms, an alkoxy group having 1 to 6 carbon atoms, an acetyl group, an amino group, an acetamido group, a cyano group, a carboxyl ester group (in which the ester portion has 1 to 6 carbon atoms), a carboxyl group, a phosphoric ester group (in which the ester portion has 2 to 6 carbon atoms), a phosphoric group, a sulfonyl group having 1 to 7 carbon atoms, a t-butoxycarbonylamino group, a 5 methylsulfoxide group, a primary amido group, or a secondary amido group),

-COCO(CH

2 )rV 3 (wherein r is an integer of 0 or 1 and V 3 is a hydrogen atom, a linear or branched alkyl group having 1 to 6 carbon atoms, an aryl group having 6 to 12 carbon atoms (in which the aryl group may have one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a carboxyl group, a carboethoxy group, a carbomethoxy group, and a cyano group), a heterocyclic ring having 1 to 9 carbon atoms (in which the heterocyclic ring may have one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, and an ethoxy group), a hydroxy group, a thiol group, a thioether group having 1 to 6 carbon atoms, an alkoxy group having 1 to 6 carbon atoms, an acetyl group, an amino group, an acetamido group, a cyano group, a carboxyl ester group (in which the ester portion has 1 to 6 carbon atoms), a carboxyl group, a primary amido group, or a secondary amido group),

-COCH=CHV

4 (wherein V 4 is an aryl group having 6 to 12 carbon atoms (in which the aryl group may have one or more substituents selected from the group essentially 6 consisting of a chlorine atom, a bromine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a carboxyl group, a carboethoxy group, a carbomethoxy group, a cyano group, a trifluoromethyl group, a methylthio group, and a phenylthio group) or a heterocyclic ring having 1 to 9 carbon atoms (in which the heterocyclic ring may have one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, and an ethoxy group),

-CO

2 G (wherein G is a hydrogen atom, a linear or branched alkyl group having 1 to 6 carbon atoms, or an aryl group having 6 to 12 carbon atoms (in which the aryl group may have one or more substituents selected from the group essentially consisting of a chlorine atom, a bromineatom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a carboxyl group, a carboethoxy group, a carbomethoxy group, a cyano group, a trifluoromethyl group, a methylthio group, and a phenylthio group), -CONHVi (wherein V 1 is a hydrogen atom, a linear or branched alkyl group having 1 to 10 carbon atoms, an aryl group having 6 to 12 carbon atoms (in which the aryl group may have one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy 7 group, an ethoxy group, a carboxyl group, a carboethoxy group, a carbomethoxy group, and a cyano group), or a heterocyclic ring having 1 to 9 carbon atoms (which heterocyclic ring may have one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, and an ethoxy group),

-NHCOV

2 (wherein V 2 is a hydrogen atom, a linear or branched alkyl group having 1 to 10 carbon atoms, an aryl group having 6 to 12 carbon atoms (in which the aryl group may have one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a carboxyl group, a carboethoxy group, a carbomethoxy group, and a cyano group), or heterocyclic ring having 1 to 9 carbon atoms (in which the heterocyclic ring may have one or more substituents selected fro m the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, and an ethoxy group), or

-(CH

2 )tJ (wherein t is an integer of from 1 to 10, J is a heterocyclic ring having 1 to 9 carbon atoms (in which the heterocyclic ring may have one or more 8 substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, and an ethoxy group), a hydroxy group, a thiol group, a thioether group having 1 to 6 carbon atoms, an alkoxy group having 1 to 6 carbon atoms, an acetyl group, an amino group, an acetamido group, a cyano group, a carboxyl ester group (in which the ester portion has 1 to 6 carbon atoms), a carboxyl group, a primary amido group, or a secondary amido group); R, is a hydrogen atom, a hydrocarbon group having 1 to 6 carbon atoms, or an acyl group having 2 to 10 carbon atoms; A is represented by the following general formula (II) or (III),

(CH

2 )n R6 R4 R wherein n is an integer of 0 or 1, R 3 and R 4 are independently a hydrogen atom, a hydrocarbon group having 1 to 15 carbon atoms (in which the hydrocarbon group may have one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, 9 a fluorine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a carboxyl group, a carboethoxy group, a carbomethoxy group, and a cyano group), a 1hydroxy-l-carboalkoxymethyl group, or a group represented by -(CH 2 )mZ (wherein m is an integer of from 1 to 6, Z is a hydroxy group, a thiol group, a thioether group having 1 to 6 carbon atoms, an alkoxy group having 1 to 6 carbon atoms, an acetyl group, an amino group, an acetamido group, a cyano group, a carboxyl ester group (in which the ester portion has 1 to 6 carbon atoms), a carboxyl group, an aldehyde group, a phosphoric group, a sulfonic group, a phosphoric ester group (in which the ester portion has 1 to 6 carbon atoms), a sulfonic ester group (in which the ester portion has 1 to 6 carbon atoms), a primary amido group, a secondary amido group, an indole group, a monosubstituted phenyl group, a disubstituted phenyl group, or a trisubstituted phenyl group (whose substituent is a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a carboxyl group, a carboethoxy group, a carbomethoxy group, and a cyano group), or R 3 and R 4 may together form -(CH 2 4 or

R

s is independently a hydrogen atom, a hydrocarbon group having 1 to 15 carbon atoms (in which the hydrocarbon group may have one or more substituents 10 selected from the group essentially consisting of,a chlorinee a atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a carboxyl group, a carboethoxy group, a carbomethoxy group, and a cyano group), a 1-hydroxy-lcarboalkoxymethyl group, or a group represented by -(CH 2

),Y

(wherein 1 is an integer of from 1 to 6, Y is a hydroxy group, a thiol group, a thioether group having 1 to 6 carbon atoms, an alkoxy group having 1 to 6 carbon atoms, an acetyl group, an amino group, an acetamido group, a cyano group, a carboxyl ester group (in which the ester portion has 1 to 6 carbon atoms), a carboxyl group, an aldehyde group, a phosphoric group, a sulfonic group, a phosphoric ester group (in which the ester portion has 1 to 6 carbon atoms), a sulfonic ester group (in which the ester portion has 1 to 6 carbon atoms), a primary amido group, a secondary amido group, an indole group, a thiophene group, a furan group, a monosubstituted phenyl group, a disubstituted phenyl group, or a trisubstituted phenyl group (whose substituent is a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a carboxyl group, a carboethoxy group, a carbomethoxy group, and a cyano group)); R 6 is independently a hydrogen atom, a halogen atom, a hydrocarbon group having 1 to 6 carbon atoms, or 11 an acyl group having 2 to 19 carbon atoms (in which the acyl group may have one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a carboxyl group, a carboethoxy group, a carbomethoxy group, and a cyano group); or R 5 and R 6 may together form -CH=CH-CH=CH- or

CO(CH

2 3 and X is O, S, CH 2 or NL (wherein L is a hydrogen atom, a linear or branched alkyl group, or L may form -CH 2

SC(CH

3 )zor 3 with R, or R 4 The present invention also provides a ketone derivative of the following general formula or a pharmacologically acceptable salt thereof:

OR,

(CH)n

R

3 RX

O

R,

(4 oe [In the above general formula R 1 is a hydrocarbon group having 3 to 19 carbon atoms (in which the hydrocarbon group may have one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a R; se P:\OPER\PDB\20436-97.074 15/3/00 -12carboxyl group, a carboethoxy group, a carbomethoxy group, and a cyano group),

-CO(CH

2 )qQ (wherein q is an integer of 0, 1 or from 3 to 10 and Q is a heterocyclic ring having 1 to 9 carbon atoms (in which the heterocyclic ring may have one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, and an ethoxy group), a hydroxy group, a thiol group, a thioether group having 1 to 6 carbon atoms, an alkoxy group having 1 to 6 carbon atoms, an acetyl group, an amino group, an acetamido group, a cyano group, a carboxyl ester group (in which the esterportion has 1 to 6 carbon atoms), a carboxyl group, a phosphoric ester group (in which the ester portion has 2 to 6 carbon atoms), a phosphoric 0 o 0 13 group, a sulfonyl group having 1 to 7 carbon atoms, a tbutoxycarbonylamino group, a methylsulfoxide group, a primary amido group, or a secondary amido group),

-COCO(CH

2 )rV 3 (wherein r is an integer of 0 or 1 and V 3 is a hydrogen atom, a linear or branched alkyl group having 1 to 6 carbon atoms, an aryl group having 6 to 12 carbon atoms (in which the aryl group may have one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a carboxyl group, a carboethoxy group, a carbimethoxy group, and a cyano group), a heterocyclic ring having 1 to 9 carbon atoms (in which the heterocyclic ring may have one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, and an ethoxy group), a hydroxy group, a thiol group, a thioether group having 1 to 6 carbon atoms, an alkoxy group having 1 to 6 carbon atoms, an acetyl group, an amino group, an acetamido group, a cyano group, a carboxyl ester group (in which the ester portion has 1 to 6 carbon atoms), a carboxyl group, a primary amido group, or a secondary amido group),

-COCH=CHV

4 (wherein V 4 is P:\OPER\PDB\20436-97.074 15/3/00 -14a heterocyclic ring having 1 to 9 carbon atoms(in which the heterocyclic ring has one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, and an ethoxy group), -C0 2 G (wherein G is an aryl group having 6 to 12 carbon atoms (in which the aryl group may have one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a carboxyl group, a carboethoxy group, a carbomethoxy group, a cyano group, a trifluoromethyl group, a methylthio group, and a phenylthio group),

-CONHV

1 (wherein V 1 is a hydrogen atom, a linear or branched alkyl group having 1 to 10 carbon atoms, an aryl group having 6 to 12 carbon atoms (in which the aryl group may have one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom t.

3 00 p 15 a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a carboxyl group, a carboethoxy group, a carbomethoxy group, and a cyano group), or a heterocyclic ring having 1 to 9 carbon atoms (in which the heterocyclic ring may have one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, and an ethoxy group)),

-NHCOV

2 (wherein V 2 is a hydrogen atom, a linear or branched alkyl group having 1 to 10 carbon atoms, an aryl group having 6 to 12 carbon atoms (in which the aryl group may have one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a carboxyl group, a carboethoxy group, a carbomethoxy group,and a cyano group) or a heterocyclic ring having 1 to 9 carbon atoms (in which the heterocyclic ring may have one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, and an ethoxy group)), or

-(CH

2 )tJ (wherein t is an integer of from 1 to 10, J is a heterocyclic ring having 1 to 9 carbon atoms (in which the heterocyclic ring may have one or more 16 substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, and an ethoxy group), a hydroxy group, a thiol group, a thioether group having 1 to 6 carbon atoms, an alkoxy group having 1 to 6 carbon atoms, an acetyl group, an amino group, an acetamido group, a cyano group, a carboxyl ester group (in which the ester portion has 1 to 6 carbon atoms), a carboxyl group, a primary amido group, or a secondary amido group);

R

2 is a hydrogen atom, a hydrocarbon group having 1 to 6 carbon atoms, or an acyl group having 2 to 10 carbon atoms; X is O, S, CH 2 or NL (wherein L forms -CH 2

SC(CH

3 2 or -(CH 2 3 with R 3 or R 4 n is an integer of 0 or 1; and or. R 3 and R 4 are independently a hydrogen atom, a hydrocarbon group having 1 to 15 carbon atoms (in which the hydrocarbon group may have one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a carboxyl group, a carboethoxy group, a carbomethoxy group, and a cyano group), a 1-hydroxy-l-carboalkoxymethyl group, or a group represented by -(CH 2 )mZ (wherein m is an integer 17 of from 1 to 6, Z is a hydroxy group, a thiol group, a thioether group having 1 to 6 carbon atoms, an alkoxy group having 1 to 6 carbon atoms, an acetyl group, an amino group, an acetamido group, a cyano group, a carboxyl ester group (in which the ester portion has 1 to 6 carbon atoms), a carboxyl group, an aldehyde group, a phosphoric group, a sulfonic group, a phosphoric ester group (in which the ester portion has 1 to 6 carbon atoms), a sulfonic ester group (in which the ester portion has 1 to 6 carbon atoms), a primary amido group, a secondary amido group, an indole group, a monosubstituted phenyl group, a disubstituted phenyl group, or a trisubstituted phenyl group (whose substituent is a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a carboxyl group, a carboethoxy group, a carbomethoxy group, and a cyano group), or R 3 and R 4 may together form 4 or The present invention also provides a ketone derivative of the following general formula or a pharmacologically acceptable salt thereof:

OR

2

R,

0

R

5 X 0 P:\OPER\PDB\20436-97.074 15/3/00 -18- [In the above general formula

R

1 is -CO(CH2)qQ (wherein q is an integer of 0, 1 or from 3 to 10 and Q is a heterocyclic ring having 1 to 9 carbon atoms (in which the heterocyclic ring has one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, and an ethoxy group), a hydroxy group, a thiol group, a thioether group having 1 to 6 carbon atoms, an alkoxy group having 1 to 6 carbon atoms, an acetyl group, an amino group, an acetamido group, a cyano group, a carboxyl ester group, a carboxyl group, a phosphoric ester group (in which the ester portion has 2 to 6 carbon atoms), a phosphoric group, a sulfonyl group having 1 to 7 carbon atoms, a tbutyoxycarbonylamino group, a methylsulfoxide group, a primary amido group, or a e 0 0*0* 0 e 19 secondary amide group), -COCO(CH,)rV 3 (wherein r is an integer of 0 or 1 and V 3 is a hydrogen atom, a linear or branched alkyl group having 1 to 6 carbon atoms, an aryl group having 6 to 12 carbon atoms (in which the aryl group may have one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a carboxyl group, a carboethoxy group, a carbomethoxy group, and a cyano group), a heterocyclic ring having 1 to 9 carbon atoms (in which the heterocyclic ring may have one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, and an ethoxy group), a hydroxy group, a thiol group, a thioether group having 1 to 6 carbon atoms, an alkoxy group having 1 to 6 carbon atoms, an acetyl group, an amino group, a acetamido group, a cyano group, a carboxyl ester group (in which ester portion has 1 to 6 carbon atoms), a carboxyl group, a primary amide group, or a secondary amide group),

-COCH=CHV

4 (wherein V 4 is a P:\OPER\PDB\20436-97.074 15/3/00 a heterocyclic ring having 1 to 9 carbon atoms (in which the heterocyclic ring has one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, and an ethoxy group),

-CO

2 G (wherein G is a hydrogen atom, a linear or branched alkyl group having 1 to 6 carbon atoms, an aryl group having 6 to 12 carbon atoms (in which the aryl group may have one or more substituents selected form the group essentially consisting of a chlorine atom, a bromine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a carboxyl group, a carboethoxy group, a carbomethoxy group, a cyano group, a trifluoromethyl group, a methylthio,group, and a phenylthio group), -CONHVI (wherein V 1 is a hydrogen atom, a linear or branched alkyl group having 1 to 10 carbon atoms, an aryl group having 6 to 12 carbon atoms (in which the aryl group 20 may have one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a carboxyl group, a carboethoxy o* 21 group, a carbomethoxy group, and a cyano group) or a heterocyclic ring having 1 to 9 carbon atoms (in which the heterocyclic ring may have one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, and an ethoxy group)),

-NHCOV

2 (wherein V 2 is a hydrogen atom, a linear or branched alkyl group having 1 to 10 carbon atoms, an aryl group having 6 to 12 carbon atoms (in which the aryl group may have one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a carboxyl group, a carboethoxy group, a carbomethoxy group, and a cyano group), or a heterocyclic ring having 1 to 9 carbon atoms (in which the he-terocyclic ring may have one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy o group, a nitro group, a methoxy group, and an ethoxy group)), or

S

22

R

2 is a hydrogen atom, a hydrocarbon group having 1 to 6 carbon atoms, or an acyl group having 2 to 10 carbon atoms; X is O, S, CH 2 or NL (wherein L is a hydrogen atom or a linear or branched alkyl group); R 5 is independently a hydrogen atom, a hydrocarbon group having 1 to 15 carbon atoms, a l-hydroxy-l-carboalkoxymethyl group, or a group represented by -(CH 2 )lY (wherein 1 is an integer of from 1 to 6 and Y is a hydroxy group, a thiol group, a thioether group having 1 to 6 carbon atoms, an alkoxy group having 1 to 6 carbon atoms, an acetyl group, an amino group, an acetamido group, a cyano group, a carboxyl ester group (in which the ester portion has 1 to 6 carbon atoms), a carboxyl group, an aldehyde group, a phosphoric group, a sulfonic group, a phosphoric ester group (in which the ester portion has 1 to 6 carbon atoms), a sulfonic ester 9S*9 o• oei 23 group (in which the ester portion has 1 to 6 carbon atoms), a primary amide group, a secondary amide group, an indole group, a thiophene group, a furan group, a monosubstituted phenyl group, a disubstituted phenyl group, or a trisubstituted phenyl group (whose substituent is a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a carboxyl group, a carboethoxy group, a carbomethoxy group, and a cyano group); R 6 is independently a hydrogen atom, a halogen atom, a hydrocarbon group having 1 to 6 carbon atoms, or an acyl group having 2 to 19 carbon atoms (in which the acyl group may have one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, an S. ethoxy group, a carboxyl group, a carboethoxy group, a carbomethoxy group, and a cyano group); or R 5 and R 6 may together form together form -CO(CH 2 3 Best Mode for Carrying Out the Invention When R 2 in general formula of the present invention is a hydrogen atom, keto-enol tautomerism shown by the following formula is included.

24 OH 0 A\R1 A_ O O In R 1 in the general formula the halogen atom may be a chlorine, a bromine, or an iodine atom; the hydrocarbon group having 1 to 19 carbon atoms may be a linear or branched alkyl group, an alkenyl group, an alkynyl group, a cycloalkyl group, an aryl group which may have one or more substituents, an alkylaryl group (whose aryl group may have one or more substituents), an arylalkyl group (whose aryl group may have one or more substituents), an arylalkenyl group (whose aryl group may have one or more substituents), or an alkenylaryl group (whose aryl may have a substituent); the alkyl group may be either linear or branched and examples thereof are methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, sec-butyl, t-butyl, n-pentyl, isopentyl, neopentyl, tpentyl, n-hexyl, n-heptyl, n-octyl, n-nonyl, n-decyl, ndodecyl, n-tetradecyl, n-pentadecyl, n-hexadecyl, nheptadecyl, 15-methylhexadecyl, 7-methylhexadecyl, and noctadecyl; and the alkenyl group may be either linear or branched, includes isomers (E and Z bodies) relating to a double bond, and examples thereof are ethenyl, 2-propenyl, 25 2-butenyl, 2-pentel, 2-hexenyl, 2-heptenyl, 2-octenyl, 2nonenyl, 2-denyl, 3-butenyl, 3-pentel, 3-hexenyl, 3heptenyl, 3-octenyl, 3-nonenyl, 3-denyl, 4-pentel, 4hexenyl, 4-heptenyl, 4-octenyl, 4-nonenyl, 4-denyl, 1,3butadienyl, 1, 3-pentadienyl, 1, 3-hexadienyl, 1,3heptadienyl, 1, 3-octadienyl, 1, 3-nonadienyl, 1,3decadienyl, 1, 4-pentadienyl, 1, 4-hexadienyl, 1,4heptadienyl, 1, 4-octadienyl, 1, 4-nonadienyl, 1,4decadienyl, 1,3, 5-hexatrienyl, 1,3, 5-heptatrienyl, 1,3,5octatrienyl, 1,3, 5-nonatrienyl, and 1,3, groups; and the cycloalkyl group includes, for example, cyclopropyl cyclobutyl, cyclohexyl, and cycloheptyl groups. Examples of the cycloalkenyl group are cycloputenyl, cyclopentenyl, cyclohexenyl, cycloheptenyl, cyclobutene-3-methyl, cyclopentene-4-methyl, cyclohexene- 3-methyl, cyclohexene-4-methyl, cyclobutene-3-ethyl, cyclopentene-4-ethyl, cyclohexene-3-ethyl, and cyclohexene-4-ethyl groups. Examples of the aryl group are phenyl, naphthyl, and biphenyl groups, in addition to aryl groups monosubstituted by a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a carboxyl group, a carboethoxy group, a carbomethoxy group, or a cyano group.

Examples of the alkylaryl group are 2-methyiphenyl, 3-methylphenyl, 4-methylphenyl, 2-ethylphenyl, 3- 26 ethyiphenyl, and 4-ethyiphenyl groups, in addition to the following groups substutued by a chlorine atom, a bromine atom, a fluorine atom, a hydroxy -group, a nitro group, a methoxy group, an ethoxy group, a carboxyl group, a carboethoxy group, a carbomethoxy group, or a cyano group: monosubstituted aryl groups-such as o-chlorophenyl, m- Chlorophenyl, p-chlorophenyl, o-bromophenyl, mbromophenyl, p-bromophenyl, o-hydroxyphenyl, mhydroxyphenyl, p-hydroxyphenyl, o-nitrophenyl, mnitrophenyl,_p-nitrophenyl, o-methoxyphenyl, mmethoxyphenyl, p-methoxyphenyl, o-cyanophenyl, mcyanophenyl, p-cyanophenyl, o-carbomethoxyphenyl, mcarbomethoxyphenyl, and p-carbomethoxyphenyl groups; disubstituted aryl groups such as o,m-dichlorophenyl, o,pdichiorophenyl, m, p-dichlorophenyl, o,m-dihydroxyphenyl, o, p-dihydroxyphenyl, m, p-dihydroxyphenyl, o, mdimethoxyphenyl, o, p-dimethoxyphenyl, m, p-dimethoxyphenyl, o-chloro-m-bromophenyl, o-chloro-m-hydroxyphenyl, ochloro-m-cyanophenyl, o-chloro-m-methoxyphenyl, o-hydroxym-chlorophenyl, and o-methoxy-m-chlorophenyl groups; and trisubstituted aryl groups such a s o,m,p-trichlorophenyl, o, m, p-tribromophenyl, o,m, p-trifluorophenyl, o-chioro--mhydroxy-p-methoxyphenyl, and o-hydroxy-m-hydroxy-pmethoxyphenyl groups; examples of the arylalkyl group are benzyl, 2-phenylethyl, 3-phenyipropyl, 2-phenyipropyl, and 27 4-phenylbutyl groups, in addition to the following groups substituted by a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a carboxyl group, a carboethoxy group, a carbomethoxy group, or a cyano group: monosubstituted aryl .groups such as o-chiorophenyl, m-chlorophenyl, pchlorophenyl, o-bromophenyl, m-bromophenyl, p-bromophenyl, o-hydroxyphenyl, m-hydroxyphenyl, p-hydroxyphenyl, onitrophenyl, m-nitrophenyl, p-nitrophenyl, omethoxyphenyl, m-methoxyphenyl, p-methoxyphenyl, ocyanophenyl, m-cyanophenyl, p-cyanophenyl, ocarbomethoxyphenyl, m-carbomethoxyphenyl, and pcarbomethoxyphenyl groups; disubstituted aryl groups such as o,m-dichlorophenyl, o,p-dichlorophenyl, m,pdichlorophenyl, o, if-dihydroxyphenyl, o, p-dihydroxyphenyl, m, p-dihydroxyphenyl, o, m-dimethoxyphenyl, o, pdimethoxyphenyl, m, p-dimethoxyphenyl, o-chloro-mbromophenyl, o-chloro-m-hydroxyphenyl, o-chloro-mcyanophenyl, o-chloro-m-methoxyphenyl, o-hydroxy-mchlorophenyl, and o-methoxy-m-chlorophenyl groups; and trisubstituted aryl groups such as o,m,p-trichlorophenyl, o,m, p-tribromophenyl, o,m, p-trifluorophenyl, o-chloro-mhydroxy-p-methoxyphenyl, and o-hydroxy-m-hydroxy-pmethoxyphenyl groups; and the arylalkenyl group includes isomers (E and Z bodies) relating to a double bond, 0 -28examples of the arylalkyl group are 2-phenylethenyl, 1phenylethenyl, 3-phenyl-2-propenyl, and 3-phenyl-1-* propenyl groups, in addition-to the following groups substituted by a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a carboxyl group, a carboethoxy group, a carbomethoxy group, or a cyano group: monosubstituted aryl groups such as o-chiorophenyl, m-chlorophenyl, pL chiorophenyl, o-bromophenyl, m-bromophenyl, p-bromophenyl, o-hydroxyphenyl, m-hydroxyphenyl, p-hydroxyphenyl, onitrophenyl, m-nitrophenyl, p-nitrophenyl, omethoxyphenyl, m-methoxyphenyl, p-methoxyphenyl, ocyanophenyl, m-cyanophenyl, p-cyanophenyl, ocarbomethoxyphenyl, m-carbomethoxyphenyl, and pcarbomethoxyphenyl groups; disubstituted aryl groups such as o,m-dichlorophenyl, o, p-dichlorophenyl, m, pdichlorophenyl, o, m-dihydroxyphenyl, o, p-dihydroxyphenyl, m, p-dihydroxyphenyl, o, m-dimethoxyphenyl, o, pdimethoxyphenyl, m, p-dimethoxyphenyl, o-chloro-mbromophenyl, o-chloro-m-hydroxyphenyl, o-chloro-mcyanophenyl, o-chlo ro-m-methoxyphenyl, o-hydroxy-mchlorophenyl, and o-methoxy-m-chlorophenyl groups; and trisubstituted aryl groups such as o,m,p-trichlorophenyl, o,m,p-tribromophenyl, o,m,p-trifluorophenyl, o-chloro-mhydroxy-p-methoxyphenyl, and o-hydroxy-m-hydroxy-p- 29 methoxyphenyl groups.

Examples of the hydrocarbon group having 1 to 6 carbon atoms in Q of -CO(CH 2 )qQ are methyl, ethyl, propyl, butyl, pentyl, hexyl, cyclobutyl, cyclopentyl, cyclohexyl, ethylene, propylene, butylene, pentylene, hexylene, acetylenyl, propynyl, butynyl, pentynyl, hexynyl, and phenyl groups; examples of the heterocyclic ring are thiophene, furan, pyrrole, tetrahydrofuran, Nmethylpyrrole, indole, imidazole, pyrrolidine, pyridine, benzothiophene, benzofuran, quinoline, isoquinoline, phthalimide, and phthalide groups; examples of the thioethr group are thiomethyl, thioethyl, thiopropyl, thiophenyl, and thiobutyl groups; examples of the alkoxy group are methoxy, ethoxy, propoxy, and phenoxy groups; examples of the carboxyl ester groups are carbomethoxy, carboethoxy, carbopropoxy, carbobutoxy, and carbophenoxy groups; examples of the phosphoric ester groups are dimethylphosphate, diethylphosphate, and dipropylphosphate groups; and examples of the sulfonyl group having 1 to 7 carbon atoms are methylsulfonyl, ethylsulfonyl, cyclohexylsulfonyl, phenylsulfonyl, pmethylphenylsulfonyl, and o-methylphenylsulfonyl groups.

Examples of the primary amido group are N-methylamido, Nethylamido, and N-propylamido groups; and examples of second amido group are N,N-dimethylamido,

N,N-

30 diethylamido, N,N-methylethylamido, and N,N-dipropylamido groups.

Examples of the linear or branched alkyl group having 1 to 6 carbon atoms in V 3 of -COCO(CH.),V 3 are methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, sec-butyl, t-butyl, n-pentyl, isopentyl, neopentyl, t-pentyl, and nhexyl groups. Examples of the aryl group are phenyl, naphthyl, and biphenyl groups, in addition to the following groups substituted by a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a carboxyl group, a carboethoxy group, a carbomethoxy group, or a cyano group: monosubstituted aryl groups such as o-chlorophenyl, mchlorophenyl, p-chlorophenyl, o-bromophenyl, mbromophenyl, p-bromophenyl, o-hydroxyphenyl, mhydroxyphenyl, p-hydroxyphenyl, o-nitrophenyl, mnitrophenyl, p-nitrophenyl, o-methoxyphenyl, mmethoxyphenyl, p-methoxyphenyl,. -cyanophenyl, mcyanophenyl, p-cyanophenyl, o-carbomethoxyphenyl, mcarbomethoxyphenyl., and p-carbomethoxyphenyl groups; disubstituted aryl groups such as o,m-dichlorophenyl, o,pdichlorophenyl, m,p-dichlorophenyl, o,m-dihydroxyphenyl, o,p-dihydroxyphenyl, m,p-dihydroxyphenyl, o,mdimethoxyphenyl, o,p-dimethoxyphenyl, m,p-dimethoxyphenyl, o-chloro-m-bromophenyl, o-chloro-m-hydroxyphenyl, o- 31 chloro-m-cyanophenyl, o-chloro-m-methoxyphenyl, o-hydroxym-chlorophenyl, and o-methoxy-m-chlorophenyl groups; and trisubstituted aryl groups such as o,m,p-trichlorophenyl, o,m,p-tribromophenyl, o,m,p-trifluorophenyl, o-chloro-mhydroxy-p-methoxyphenyl, and o-hydroxy-m-hydroxy-pmethoxyphenyl groups. Examples of the heterocyclic ring are thiophene, furan, pyrrole, tetrahydrofuran, Nmethylpyrrole, indole, imidazole, pyrrolidine, pyridine, benzothiophene, benzofuran, quinoline, isoquinoline, phthalimide, and phthalide groups; examples of the thioether group are thiomethyl, thioethyl, thiopropyl, thiophenyl, and thiobutyl groups; examples of the alkoxy group are methoxy, ethoxy, propoxy, and phenoxy groups; examples of the carboxyl ester group are carbomethoxy, carboethoxy, carbopropoxy, carbobutoxy, and carbophenoxy groups; examples of the phosphoric ester group are dimethylphosphate, diethylphosphate, and dipropylphosphate groups; examples of the primary amido group are Nmethylamido, N-ethylamido, and N-propylamido groups; and examples of the second amido group are N,N-dimethylamido, N,N-diethylamido, N,N-methylethylamido, and N,Ndipropylamido groups.

Examples of the aryl group in V 4 of -COCH=CHV 4 are phenyl, naphthyl, and biphenyl groups, in addition to the following groups substituted by a chlorine atom, a bromine 32 atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group,, a carboxyl group, a carboethoxy group, a carbomethoxy group, or a cyano group: monosubstituted aryl groups such as o-chlorophenyl,, mchiorophenyl, p-chlorophenyl, o-bromophenyl, mbromophenyl, p-bromophenyl, o-hydroxyphenyl, mhydroxyphenyl, p-hydroxyphenyi, o-nitrophenyl, mnitrophenyl, p-nitrophenyl, o-methoxyphenyl, mmethoxyphenyl, p-methoxyphenyl, o-cyanophenyl, mcyanophenyl, p-cyanophenyl, o-carbomethoxyphenyl, mcarbomethoxyphenyl, and p-carbomethoxyphenyl groups; disubstituted aryl groups such as o,m-dichlorophenyl, o,pdichiorophenyl, m, p-dichlorophenyl, o,m-dihydroxyphenyl, o, p-dihydroxyphenyl, m, p-dihydroxyphenyl, o, mdimethoxyphenyl, o, p-dimethoxyphenyl, m, p-dimethoxyphenyl, o-chloro-m-bromophenyl, o-chloro-m-hydroxyphenyl, ochloro-m-cyanophenyl, o-chloro-m-methoxyphenyli o-hydroxym-chlorophenyl, and o-methoxy-m-chlorophenyl groups; and trisubstituted aryl groups such as o,m,p-trichlorophenyl, o,m, p-tribro-mophenyl, o,m,p-trifluorophenyl, o-chloro-mhydroxy-p-methoxyphenyl, and o-hydroxy-m-hydroxy-pmethoxyphenyl groups. Examples of the heterocyclic ring are thiophene, furan, pyrrole, tetrahydrofuran, Nmethylpyrrole, indole,.imidazole, pyrrolidine, pyridine, benzothiophene, benzofuran, quinoline, isoquinoline, 33 phthalimide, and phthalide groups.

Examples of the linear or branched alkyl group having 1 to 6 carbon atoms in G of -CO 2 G are propyl, butyl, and pentyl groups. Examples of the aryl group are phenyl, naphthyl, and biphenyl groups, in addition to the following groups substituted by a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a carboxyl group, a carboethoxy group, a carbomethoxy group, or a cyano group: monosubstituted aryl groups such as o-chlorophenyl, mchlorophenyl, p-chlorophenyl, o-bromophenyl, mbromophenyl, p-bromophenyl, o-hydroxyphenyl, mhydroxyphenyl, p-hydroxyphenyl, o-nitrophenyl, mnitrophenyl, p-nitrophenyl, o-methoxyphenyl, mmethoxyphenyl, p-methoxyphenyl, o-cyanophenyl, mcyanophenyl, p-cyanophenyl, o-carbomethoxyphenyl, mcarbomethoxyphenyl, and p-carbomethoxyphenyl groups; disubstituted aryl groups such as o,m-dichlorophenyl, o,pdichlorophenyl, m,p-dichlorophenyl, o,m-dihydroxyphenyl, o,p-dihydroxyphenyl, m,p-dihydroxyphenyl, o,mdimethoxyphenyl, o, p-dimethoxyphenyl, m,p-dimethoxyphenyl, o-chloro-m-bromophenyl, o-chloro-m-hydroxyphenyl, ochloro-m-cyanophenyl, o-chloro-m-methoxyphenyl, o-hydroxym-chlorophenyl, and o-methoxy-m-chlorophenyl groups; and trisubstituted aryl groups such as o,m,p-trichlorophenyl, 34 o,m,p-tribromophenyl, o,m,p-trifluorophenyl, o-chloro-mhydroxy-p-methoxyphenyl, and o-hydroxy-m-hydroxy-pmethoxyphenyl groups.

Examples of the linear or branched -alkyl group having 1 to 6 carbon atoms in V, of -CONHV, are methyl, ethyl, npropyl, isopropyl, n-butyl, isobutyl, sec-butyl, t-butyl, n-pentyl, isopentyl, neopentyl, t-pentyl, n-hexyl, nheptyl, n-octyl, n-nonyl, and n-decyl groups. Examples of the aryl group are phenyl, naphthyl, and biphenyl groups, in addition to the following groups substituted by a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a carboxyl group, a carboethoxy group, a carbomethoxy group, or a cyano group: monosubstituted aryl groups such as ochlorophenyl, m-chlorophenyl, p-chlorophenyl, obromophenyl,* m-bromophenyl, p-bromophenyl, ohydroxyphenyl, m-hydroxyphenyl, p-hydroxyphenyl, onitrophenyl, m-nitrophenyl, p-nitrophenyl, omethoxyphenyl, m-methoxyphenyl, p-methoxyphenyl, ocyanophenyl, m-cyanophenyl, p-cyanophenyl, ocarbomethoxyphenyl, m-carbomethoxyphenyl, and pcarbomethoxyphenyl groups; disubstituted aryl groups such as o,m-dichlorophenyl, o,p-dichlorophenyl, m,pdichlorophenyl, o,m-dihydroxyphenyl, o,p-dihydroxyphenyl, m,p-dihydroxyphenyl, o,m-dimethoxyphenyl, o,pdimethoxyphenyl, m,p-dimethoxyphenyl, o-chloro-mbromophenyl, o-chloro-m-hydroxyphenyl, o-chloro-mcyanophenyl, o-chloro-m-methoxyphenyl, o-hydroxy-mchiorophenyl, and o-methoxy-m-chlorophenyl groups; and trisubstituted aryl groups such as o,m,p-trichlorophenyl, o,m,p-tribromophenyl, o,m,p-trifluorophenyl, o-chloro-mhydroxy-p-methoxyphenyl, and o-hydroxy-m-hydroxy-pmethoxyphenyl groups. Examples of the heterocyclic ring are thiophene, furan, pyrrole, tetrahydrofuran, Nmethylpyrrole, indole, imidazole, pyrrolidine, pyridine, benzothiophene, benzofuran, quinoline, isoquinoline, phthalimide, and phthalide groups.

Examples of the linear or branched alkyl group having 1 to 10 carbon atoms in V 2 of -NHCOV 2 are methyl, ethyl, npropyl, isopropyl, n-butyl, isobutyl, sec-butyl, t-butyl, n-pentyl, isopentyl, neopentyl, t-pentyl, n-hexyl, nheptyl, n-octyl, n-nonyl, and n-decyl groups. Examples of the aryl group are phenyl, naphthyl, and biphenyl groups, in addition to the following groups substituted by a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a carboxyl group, a carboethoxy group, a carbomethoxy group, or a cyano group: monosubstituted aryl groups such as ochlorophenyl, m-chlorophenyl, p-chlorophenyl, obromophenyl, m-bromophenyl,' p-bromophenyl, o- 36 hydroxyphenyl, m-hydroxyphenyl, p-hydroxyphenyl, onitrophenyl, m-nitrophenyl, p-nitrophenyl, omethoxyphenyl, m-methoxyphenyl, p-methoxyphenyl, ocyanophenyl, m-cyanophenyl, p-cyanophenyl, acarbomethoxyphenyl, m-carbomethoxyphenyl, and pcarbomethoxyphenyl groups; disubstituted aryl groups such as o,m-dichlorophenyl, o, p-dichlorophenyl, m, pdichiorophenyl, o, m-dihydroxyphenyl, a, p-dihydroxyphehyl, m, p-dihydroxyphenyl, a, m-dimethoxyphenyl, a, pdimethoxyphenyl, m, p-dimethoxyphenyl, o-chloro-mbromophenyl, o-chloro-m-hydroxyphenyl, o-chloro-mcyanophenyl, o-chloro-m-methoxyphenyl, o-hydroxy-mchiorophenyl, and o-methoxy-m-chlorophenyl groups; and trisubstituted aryl groups such as o,m,p-trichlorophenyl, o,m, p-tribromophenyl, o,m, p-trifluorophenyl, o-chloro-mhydroxy-p-methoxyphenyl, and o-hydroxy-m-hydroxy-pmethoxyphenyl groups. Examples of the heterocyclic ring are thiophene, furan, pyrrole, tetrahydrofuran, Nmethylpyrrole, indole, imidazole, pyrrolidine, pyridine, benzothiophene, benzofuran, quinoline, isoquinoline, phthalimide, and phthalide groups.

Examples of the heterocyclic ring in J of -(CH 2 are thiophene, furan, pyrrole, tetrahydrofuran, Nmethylpyrrole, indole, imidazole, pyrrolidine, pyridine, benzothiophene, benzofuran, quinoline, isoquinoline, 37 phthalimide, and phthalide groups; examples of the thioether group are thiomethyl, thioethyl, thiopropyl, thiophenyl, and thiobutyl groups; examples of the alkoxy group are methoxy, ethoxy, propoxy, and phenoxy groups; examples of the carboxyl ester group are carbomethoxy, carboethoxy, carbopropoxy, carbobutoxy, and carbophenoxy groups; examples of the primary amido group are Nmethylamido, N-ethylamido, and N-propylamido groups; and examples of the second amido group are N,N-dimethylamido, N,N-diethylamido, N,N-methylethylamido, and N,Ndipropylamido groups.

Examples of the hydrocarbon group having 1 to 6 carbon atoms in R 2 in the general formula are methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, sec-butyl, n-pentyl, isopentyl, t-pentyl, and n-hexyl groups, and the acyl group having 2 to 10 carbon atoms may be either saturated or unsaturated and either linear or branched; examples thereof are acetyl, propionyl, butyryl, valeryl, isovaleryl, pivaloyl, hexanoyl, heptanoyl, octanoyl, benzoyl, toluoyl, and naphthoyl groups.

Examples of the hydrocarbon group having 1 to carbon atoms in R 3 and R 4 in the general formula (II) are a linear or branched alkyl group, an alkenyl group, an alkynyl group, a cycloalkyl group, a cycloalkenyl group, an aryl group which may have a substituent, an alkylaryl 38 group (whose'aryl group may have one or more substituents), an-arylalkyl group (whose aryl group may have one or more substituents), an arylalkenyl group (whose aryl group may have one or more substituents), or an alkenylaryl group (whose aryl group may have one or more substituents); the alkyl group may be either linear or branched and includes methyl-, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, sec-butyl, t-butyl, npentyl, isopentyl, neopentyl, t-pentyl, n-hexyl, n-heptyl, n-octyl, n-nonyl, and n-decyl groups; and the alkenyl group may be either linear or branched, includes isomers (E and Z bodies) relating to a double bond, and examples thereof are, ethenyl, 2-propenyl, 2-butenyl, 2-pentel, 2hexenyl, 2-heptenyl, 2-octenyl, 2-nonenyl, 2-denyl, 3butenyl, 3-pentel, 3-hexenyl, 3-heptenyl, 3-octenyl, 3nonenyl, 3-denyl, 4-pentel, 4-hexenyl, 4-heptenyl, 4octenyl, 4-nonenyl, 4-denyl, 1,3-butadienyl, 1,3pentadienyl, 1,3-hexadienyl, 1,3-heptadienyl, 1,3octadienyl, 1,3-nonadienyl, 1,3-decadienyl, 1,4pentadienyl, 1,4-hexadienyl, 1,4-heptadienyl, 1,4octadienyl, 1,4-nonadienyl, 1,4-decadienyl,' 1,3,5hexatrienyl, 1,3,5-heptatrienyl, 1,3,5-octatrienyl, 1,3,5nonatrienyl, and 1,3,5-decatrienyl groups.

The cycloalkyl group may be, for example, cyclopropyl, cyclobutyl, cyclohexyl, and cycloheptyl 0 -39groups. Examples of the'aryl group are phenyl, naphthyl, and biphenyl groups, in addition to the following groups substituted by a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a cyano group, a carboxyl group, a carboethoxy group, or a carbomethoxy group: monosubstituted aryl groups such as o-chiorophenyl, mchiorophenyl, p-chlorophenyl, o-bromophenyl, m-' bromophenyl, p-bromophenyl, o-hydroxyphenyl, mhydroxyphenyl, p-hydroxyphenyl, o-nitrophenyl, mnitrophenyl, p-nitrophenyl, o-methoxyphenyl, mmethoxyphenyl, p-methoxyphenyl, o-cyanophenyl, mcyanophenyl, p-cyanophenyl, o-carbomethoxyphenyl, mcarbomethoxyphenyl, and p-carbomethoxyphenyi groups; disubstituted aryl groups such as o,m-dichlorophenyl, o,pdichiorophenyl, m, p-dichlorophenyl, o, m-dihydroxyphenyl, o, p-dihydroxyphenyl, m, p-dihydroxyphenyl, o,mdimethoxyphenyl, o, p-dimethoxyphenyl, m, p-dimethoxyphenyl, o-chloro-m-bromophenyl, o-chloro-m-hydroxyphenyl, ochloro-m-cyanophenyl, o-chloro-m-methoxyphenyl, o-hydroxym-chlorophenyl, and o-methoxy-m-chlorophenyl groups; and trisubstituted aryl groups such as o,m,p-trichlorophenyl, o,m, p-tribromophenyl, o,m, p-trifluorophenyl, o-chloro-mhydroxy-p-methoxyphenyl, and o-hydroxy-m-hydroxy-pmethoxyphenyl groups.

40 Examples of the alkylaryl group are 2-methyiphenyl, 3-methyiphenyl, 4-methyiphenyl, 2-ethyiphenyl, 3ethyiphenyl, and 4-ethyiphenyl groups, in addition to the following groups substituted by a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group,-a.

methoxy group, an ethoxylgroup, a carboxyl group, a carboethoxy group, a carbomethoxy group, or a cyano group: monosubstituted aryl groups such as o-chlorophenyl, mchlorophenyl, p-chlorophenyl, o-bromophenyl, mbromophenyl, p-bromophenyl, o-hydroxyphenyl, mhydroxyphenyl, p-hydroxyphenyl, o-nitrophenyl, mnitrophenyl, p-nitrophenyl, o-methoxyphenyl, mmethoxyphenyl, p-methoxyphenyl, o-.cyanophenyl, mcyanophenyl, p-cyanophenyl, o-carbomethoxyphenyl, mcarbomethoxyphenyl, and p-carbomethoxyphenyl groups; disubstituted aryl groups such as o,m-dichlorophenyl, o,pdichlorophenyl, m, p-dichlorophenyl, o, m-dihydroxyphenyl, o, p-dihydroxyphenyl, m, p-dihydroxyphenyl, o, mdimethoxyphenyl, o, p-dimethoxyphenyl, m, p-dimethoxyphenyl, o-chloro-m-bromophenyl, o-chloro-m-hydroxyphenyl, ochioro-m-cyanophenyl, o-chloro-m-methoxyphenyl, o-hydroxym-chlorophenyl, and o-methoxy-m-chlorophenyl groups; and trisubstituted aryl groups such as o,m,p-trichlorophenyl, o,m, p-tribromophenyl, o,m, p-trifluorophenyl, o-chloro-mhydroxy-p-methoxyphenyl, and o-hydroxy-m-hydroxy-p- 0 -41methoxyphenyl groups.

Examples of the-arylalkyl group are benzyl, 2phenylethyl, 3-phenyipropyl, 2-phenyipropyl, and 4phenylbutyl groups, in addition to the following groups substituted by a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a carboxyl group, a carboethoxy group,-a carbomethoxy group, or a cyano group: monosubstituted aryl groups such as o-chiorophenyl, m-chlorophenyl, pchlorophenyl, o-bromophenyl, m-bromophenyl, p-bromophenyl, o-hydroxyphenyl, m-hydroxyphenyl, p-hydroxyphenyl, onitrophenyl, m-nitrophenyl, p-nitrophenyl, omethoxyphenyl, m-methoxyphenyl, p-methoxyphenyl, ocyanophenyl, m-cyanophenyl, p-cyanophenyl, ocarbomethoxyphenyl, m-carbomethoxyphenyl, and pcarbomethoxyphenyl groups; disubstituted aryl groups such as o, m-dichlorophenyl, o, p-dichlorophenyl, m, pdichlorophenyl, o, m-dihydroxyphenyl, o, p-dihydroxyphenyl, m, p-dihydroxyphenyl, o, m-dimethoxyphenyl, ,o pdimethoxyphenyl, m, p-dimethoxyphenyl, o-chloro-mbromophenyl, o-chloro-m-hydroxyphenyl, o-chloro-mcyanophenyl, o-chloro-m-methoxyphenyl, o-hydroxy-mchlorophenyl, and o-methoxy-m--chlorophenyl groups; and trisubstituted aryl groups such as o,m,p-trichlorophenyl, o,m,p-tribromop henyl, o,m,p-trifluorophenyl, o-chloro-m- 42 hydroxy-p-methoxyphenyl, and o-hydroxy-m-hydroxy-pmethoxyphenyl groups; and the arylalkenyl groups includes isomers (E and Z bodies) relating to a double bond, such as 2-phenylethenyl, 1-phenylethenyl, 3-phenyl-2-propenyl, and 3-phenyl-l-propenyl groups.

Examples of the l-hydroxy-l-carboalkoxymethyl group are 1-hydroxy-1-carbomethoxymethyl, 1-hydroxy-1carbomethoxymethyl, and 1-hydroxy-1-carbopropoxymethyl groups.

Examples of the thioether group in Z in -(CH 2 )mZ are thiomethyl, thioethyl, thiopropyl, thiophenyl, and thiobutyl groups; examples of the alkoxy group are methoxy, ethoxy, propoxy, and phenoxy groups; examples of the carboxyl ester group are carbomethoxy, carboethoxy, carbopropoxy, carbobutoxy, and carbophenoxy groups;.

examples of the phosphoric ester group are methylphosphate, ethylphosphate, propylphosphate, butylphosphate, and phenylphosphate group; examples of the primary amido groups are N-methylamido, N-ethylamido, and N-propylamido groups; examples of the second amido group are N,N-dimethylamido, N,N-diethylamido,

N,N-

methylethylamido, and N,N-dipropylamido groups; examples of the monosubstituted phenyl group are the phenyl groups monosubstituted by a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy 43 group, an ethoxy group, a cyano group, a carboxyl group, a carboethoxy group, or a carbomethoxy group, such as ochiorophenyl, m-chlorophenyl, p-chlorophenyl, obromophenyl, m-bromophenyl, p-bromophenyl, ohydroxyphenyl, m-hydroxyphenyl, p-hydroxyphenyl, onitrophenyl, m-nitrophenyl, p-nitrophenyl, omethoxyphenyl, m-methoxyphenyl, p-methoxyphenyl, ocyanophenyl, m-cyanophenyl, p-cyanophenyl, ocarbomethoxyphenyl, m-carboethoxyphenyl, and pcarbomethoxyphenyl groups; examples of the disubstituted phenyl group are phenyl groups disubstituted by a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a cyano group, a carboxyl group, a carboethoxy group, or a carbomethoxy group, such as o,m-dichlorophenyl, o,pdichiorophenyl, m,p-dichlorophenyl, o,m-dihydroxyphenyl, o,p-dihydroxyphenyl, m,p-dihydroxyphenyl, o,mdimethoxyphenyl, o,p-dimethoxyphenyl, m,p-dimethoxyphenyl, o-chloro-m-bromophenyl, o-chloro-m-hydroxyphenyl, ochloro-m-cyanophenyl, o-chloro-m-methoxyphenyl, o-hydroxym-chlorophenyl, and o-methoxy-m-chlorophenyl groups; and examples of the trisubstituted phenyl group are phenyl groups trisubstituted by a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a cyano group, a carboxyl group, a 44 carboethoxy group, or a carbomethoxy group, such as o,m,ptrichlorophenyl, o,m,p-tribromophenyl, o,m,ptrifluorophenyl, o-chioro-m-hydroxy-p-methoxyphenyl, and o-hydroxy-m-hydroxy-p-methoxyphenyl groups.

Examples of the hydrocarbon group having 1 to carbon atoms in R 5 in general formula (III) are a linear or branched alkyl group, an alkenyl group, an alkynyl group, a cycloalkyl group, a cycloalkenyl group, an aryl group which may have one or more substituents, an alkylaryl group (whose aryl group may have one or more substituents), an arylalkyl group (whose aryl group may have one or more substituent), an arylalkenyl group (whose aryl group may have one or more substituent), or an alkenylaryl group (whose aryl group may have one or more substituent); the alkyl group may be either linear or branched and includes methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, sec-butyl, t-butyl, n-pentyl, isopentyl, neopentyl, t-pentyl, n-hexyl, n-heptyl, noctyl, n-nonyl, and n-decyl groups; and the alkenyl group may be either linear or branched, includes isomers (E and Z bodies) relating to a double bond, and example thereof are ethenyl, 2-propenyl, 2-butenyl, 2-pentel, 2-hexenyl, 2-heptenyl, 2-octenyl, 2-nonenyl, 2-denyl, 3-butenyl, 3pentel, 3-hexenyl, 3-heptenyl, 3-octenyl, 3-nonenyl, 3denyl, 4-pentel, 4-hexenyl, 4 -heptenyl, 4-octenyl, 4- 45 nonenyl, 4-denyl, 1,3-butadienyl, 1,3-pentadienyl, 1,3hexadienyl, 1,3-heptadienyl, 1,3-octadienyl, 1,3nonadienyl, 1,3-decadienyl, 1,4-pentadienyl, 1,4hexadienyl, 1,4-heptadienyl, 1,4-octadienyl, 1,4nonadienyl, 1,4-decadienyl, 1,3, 5-hexatrienyl, 1,3,5heptatrienyl, 1,3,5-octatrienyl, 1,3,5-nonatrienyl, and 1,3,5-decatrienyl groups. The cycloalkyl group includes, for example, cyclopropyl cyclobutyl, cyclohexyl, and cycloheptyl groups.

Examples of the aryl group are phenyl, naphthyl, and biphenyl groups, in addition to the following groups substituted by a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a cyano group, a carboxyl group, a carboethoxy group, or a carbomethoxy group: monosubstituted aryl groups such as o-chlorophenyl, mchlorophenyl, p-chlorophenyl, o-bromophenyl, mbromophenyl, p-bromophenyl, o-hydroxyphenyl, mhydroxyphenyl, p-hydroxyphenyl, o-nitrophenyl, mnitrophenyl, p-nitrophenyl, o-methoxyphenyl, mmethoxyphenyl, p-methoxyphenyl, o-cyanophenyl, mcyanophenyl, p-cyanophenyl, o-carbomethoxyphenyl, mcarbomethoxyphenyl, and p-carbomethoxyphenyl groups; disubstituted aryl groups such as o,m-dichlorophenyl, o,pdichlorophenyl, m,p-dichlorophenyl, o,m-dihydroxyphenyl, 46 o, p-dihydroxyphe6nyl, m, p-dihydroxyphenyl, o, mdimethoxyphenyl, o, p-dimethoxyphenyl, m, p-dimethoxyphenyl, o-chloro-m-bromophenyl, o-chloro-m-hydroxyphenyl, ochloro-m-cyanophenyl, o-chloro-m-methoxyphenyl, o-hydroxym-chlorophenyl, and o-methoxy-m-chlorophenyl groups; and trisubstituted aryl groups such as o,m,p-trichlorophenyl, o,m, p-tribromophenyl, o,m, p-trifluorophenyl, o-chloro-mhydroxy-p-methoxyphenyl, and o-hydroxy-m-hydroxy-pmethoxyphenyl groups.

Examples of the alkylaryl group are 2-methyiphenyl, 3-methyiphenyl, 4-methyiphenyl,, 2-ethyiphenyl, 3ethyiphenyl, and 4-ethyiphenyl groups, in addition to the following groups substituted by a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a carboxyl group, a carboethoxy group, a carbomethoxy group, or a cyano group: monosubstituted aryl groups such as o-chlorophenyl, mchlorophenyl, p-chlorophenyl, o-bromophenyl, Mbromophenyl, p-bromophenyl, o-hydroxyphenyl, mhydroxyphenyl, p-hydroxyphenyl, o-nitrophenyl, mnitrophenyl, p-nitrophenyl, o-methoxyphenyl, mmethoxyphenyl, p-methoxyphenyl, o-cyanophenyl, mcyanophenyl, p-cyanophenyl, o-carbomethoxyphenyl, mcarbomethoxyphenyl, and p-carbomethoxyphenyl groups; disubstituted aryl groups such as o,m-dichlorophenyl, o,p- 47 dichiorophenyl, m, p-dichlorophenyl, o, m-dihydroxyphenyl, o, p-dihydroxyphenyl, m, p-dihydroxyphenyl, 0,midimethoxyphenyl, o, p-dimethoxyphenyl, m, p-dimethoxyphenyl, o-chloro-m-bronophenyl, o-chloro-m-hydroxyphenyl, ochioro-in-cyanophenyl, o-chloro-m-methoxyphenyl, o-hydroxym-chlorophenyl, and o-iethoxy-m-chlorophenyl groups; and trisubstituted aryl groups such as o,m,p-trichlorophenyl, o,m, p-tribromophenyl.' o,rn, p-trifluorophenyl, o-chloro-mhydroxy-p-methoxyphenyl, and o-hydroxy-m-hydroxy-pmethoxyphenyl groups.

Examples of the arylalkyl group are benzyl, 2phenylethyl, 3-phenyipropyl, 2-phenyipropyl, and 4phenylbutyl groups, in addition to the following groups substituted by a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a cyano group, a carboxyl group, a carboethoxy group, or a carbomethoxy group: monosubstituted aryl groups such as o-chiorophenyl, mchiorophenyl, p-chlorophenyl, o-bromophenyl, mbromophenyl, p-bromophenyl, o-hydroxyphehyl, mhydroxyphenyl, p-hydroxyphenyl, o-nitrophenyl, mnitrophenyl, p-nitrophenyl, o-methoxyphenyl, mmethoxyphenyl, p-methoxyphenyl, o-cyanophenyl, incyanophenyl, p-cyanophenyl, o-carbomethoxyphenyl, mcarboinethoxyphenyl, and p-carbomethoxyphenyl groups; 48 disubstituted aryl groups such as o,m-dichlorophenyl, b,pdichiorophenyl, m, p-dichlorophenyl, o, m-dihydroxyphenyl, o, p-dihydroxyphenyl, m, p-dihydroxyphenyl, o, mdimethoxyphenyl, a ,p-dimethoxyphenyl, m, p-dimethoxyphenyl, o-chloro-m-bromophenyl, o-chloro-m-hydroxyphenyl, ochloro7m-cyanophenyl, o-chloro-m-methoxyphenyl, o-hydroxym-chlorophenyl, and o-methoxy-m-chlorophenyl groups; and trisubstituted aryl groups such as o,m,p-trichlorophenyl, o, m,p-tribromophenyl, o,m, p-trifluorophenyl, o-chloro-mhydroxy-p-methoxyphenyl, and o-hydroxy-m-hydroxy-pmethoxyphenyl groups; and the arylalkenyl group includes isomers (E and Z bodies) relating to a double bond, such as 2-phenylethenyl, 1-phenylethenyl, 3-phenyl-2-propenyl, and 3-phenyl-l-propenyl groups.

Examples of the thioether group in Y in -(CH 2 )ly are thiomethyl, thioethyl, thiopropyl, thiophenyl, and thiobutyl groups; examples of the alkoxy group are methoxy, ethoxy, propoxy, and phenoxy groups; examples of the carboxyl ester group are carbomethoxy, carboethoxy, carbopropoxy, carbobutoxy, and carbophenoxy groups; examples of the phosphoric ester group are methylphosphate, ethyiphosphate, propylphosphate,' butylphosphate, and phenyiphosphate groups; examples of the sulfonic ester group are methylsulf ate andethylsulf ate groups; examples of the primary amido'group 49 are N-methylamido, N-ethylamido, and N-propylamido groups; and examples of the second amido group are N,Ndimethylamido, N, N-diethylamido, N, N-methylethylamido, and N,N-dipropylamido groups.

Examples of the monosubstituted phenyl group are phenyl groups monosubstituted by a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a cyano group, a carboxyl group, a carboethoxy group, or a carbomethoxy group, such as o-chlorophenyl, m-chlorophenyl, pchlorophenyl, o-bromophenyl, m-bromophenyl, p-bromophenyl, o-hydroxyphenyl, m-hydroxyphenyl, p-hydroxyphenyl, onitrophenyl, m-nitrophenyl, p-nitrophenyl, omethoxyphenyl, m-methoxyphenyl, p-methoxyphenyl, ocyanophenyl, m-cyanophenyl, p-cyanophenyl, ocarbomethoxyphenyl, m-carbomethoxyphenyl, and pcarbomethoxyphenyl groups; examples of the disubstituted phenyl group are phenyl groups disubstituted by a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group,. a methoxy group, an ethoxy group, a cyano group, a carboxyl group, a carboethoxy group, or a carbomethoxy group, such as o,m-dichlorophenyl, o,pdichlorophenyl, m,p-dichlorophenyl, o,m-dihydroxyphenyl, o,p-dihydroxyphenyl, m,p-dihydroxyphenyl, o,mdimethoxyphenyl, o, p-dimethoxyphenyl, m, p-dimethoxyphenyl, 50 o-chloro-m-bromophenyl, o-chloro-m-hydroxyphenyl, ochloro-m-cyanophenyl, o-chloro-m-methoxyphenyl, o-hydroxym-chlorophenyl, and o-methoxy-m-chlorophenyl groups; and examples of the trisubstituted phenyl group are phenyl groups trisubstituted by a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a cyano group, a carboxyl group, a carbbethoxy group, or a carbomethoxy group, such as o,m,ptrichlorophenyl, o,m,p-tribromophenyl, o,m,ptrifluorophenyl, o-chloro-m-hydroxy-p-methoxyphenyl, and o-hydroxy-m-hydroxy-p-methoxyphenyl.

In R 6 in the general formula the halogen atom may be a chlorine, a bromine, or an iodine atom, and the hydrocarbon group having 1 to 6 carbon atoms may be methyl, ethyl, propyl, butyl, pentyl, hexyl, isopropyl, cyclopropyl, and phenyl groups. The acyl group having 2 to 19 carbon atoms may be either saturated or unsaturated and either linear or branched; examples thereof are acetyl, propionyl, butyryl, valeryl, isovaleryl, bivaloyl, hexanoyl, heptanoyl, octanoyl, benzoyl, toluoyl, and naphthoyl groups, in addition to acyl groups having the following groups substituted by a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a carboxyl group, a carboethoxy group, a carbomethoxy group, or a cyano group: 51 monosubstituted aryl groups such as o-chlorophenyl, mchiorophenyl, p-chlorophenyl, o-bromophenyl, mbromophenyl, p-bromophenyl, o-hydroxyphenyl, mhydroxyphenyl, p-hydroxyphenyl, o-nitrophenyl, mnitrophenyl, p-nitrophenyl, o-methoxyphenyl, mmethoxyphenyl, p-methoxyphenyl, o-cyanophenyl, mcyanophenyl, p-cyanophenyl, o-carbomethoxyphenyl, Incarbomethoxyphenyl, and p-carbomethoxyphenyl groups; disubstituted aryl groups such as o,m-dichlorophenyl, o,pdichiorophenyl, m, p-dichlorophenyl, o, m-dihydroxyphenyl, o, p-dihydroxyphenyl, m, p-dihydroxyphenyl, o, mdimethoxyphenyl, o ,p-dimethoxyphenyl, m, p-dimethoxyphenyl, o-chloro-m-bromophenyl, o-chloro-m-hydroxyphenyl, ochloro-m-cyanophenyl, o-chloro-m--methoxyphenyl, o-hydroxym-chlorophenyl, and o-methoxy-m-chlorophenyl groups; and trisubstituted aryl groups such as o,m,p-trichlorophenyl, o,m, p-tribromophenyl, o,m, p-trifluorophenyl, o-chloro-mhydroxy-p-methoxyphenyl, and o-hydroxy-m-hydroxy-pmethoxyphenyl groups.

Examples of the linear or branched alkyl group having 1 to 6 carbon atoms in L in NL are methyl, ethyl, npropyl, isopropyl, n-butyl, isobutyl, sec-butyl, n-pentyl, isopentyl, t-pentyl, and n-hexyl groups.

52 Examples of pharmacologically acceptable salts are base addition salts and acid addition salts. Base addition salts are those which retain biological availability and the properties of free bases and which do not have biologically adverse effects or other adverse effects; examples thereof are salts obtained from inorganic bases such as sodium, potassium, lithium, ammonium, calcium, magnesium, and the like. Furthermore, pharmacologically acceptable salts include salts obtained from organic bases, for example, substituted amines such as primary amine, secondary amine, tertiary amine, naturally substituted amine, cyclic amine, and basic ion exchange resin, and practically, isopropylamine, trimethylamine, diethylamine, tripropylamine, ethanol amine, 2-dimethylamino ethanol, 2-diethylamino ethanol, trimetamine, dicyclohexylamine, lysine, arginine, histidine, caffeine, procaine, hydravamine, choline, and betaine, and practically, isopropylamine, trimethylamine, diethylamine, tripropylamine, ethanol amine, 2dimethylamino ethanol, 2-diethylamino ethanol, trimetamine, dicyclohexylamine, lysine, arginine, histidine, caffeine, procaine, hydravamine, choline, betaine, ethylenediamine, glucosamine, methylglucamine, theobromine, purine, piperazine, piperidine, Nethylpiperidine, ornithine, and polyamine resin. However, 53 pharmacologically acceptable salts in the present invention are not restricted to the above salts. Acid addition salts are those which retain biological availability and the properties of free acids and which do not have biologically adverse effects or other adverse effects; examples thereof are inorganic salts such as hydrochlorides, sulfates, nitrates, hydrobromates, hydrogen tetrafluoroborate, phosphates, and perchlorates.

Furthermore, pharmacologically acceptable salts include, for example, oxalates, tartrates, lactates, and acetates as salts of organic acids. However, pharmacologically acceptable salts of the present invention are not limited to these salts.

When compounds of the present invention have an asymmetric carbon in their molecule, various types of optical isomers exist, furthermore, when compounds of the present invention have at least two asymmetric carbons, various types of diastereomers exist. The present invention also includes such optical isomers as well as each isomer. Moreover, the present invention includes stereoisomers.

Lactones of the present invention can be produced by a known method. For example, they can be produced by the methods disclosed in J. Chem. Soc. Perkin Trans. I. 121- 129 (1987), J. Org. Chem., 59, 488-490 (1994), Bull. Chem.

54 Soc. Japan, 52, 3601-3605 (1979), J. Chem. Soc. Perkin Trans. I. 1225-1231 (1987), Tetrahedron Lett., 5143-5146 (1983), Chem. Pharm. Bull., 38, 94-98 (1990), Chem. Pharm.

Bull., 34, 5188-5190 (1986), Tetrahedron Lett., 8285-8296 (1991), Tetrahedron Lett., 4807-4810 (1988), Chem. Pharm.

Bull., 34(12), 5188 (1986), J. Chem. Soc., 1501 (1968), J. Org. Chem., 59, 4749 (1994), Chem. Pharm.

Bull., 29(10), 2762 (1981), J. Chem. Soc., 4483 (1963), J.

Am. Chem. Soc., 78, 3201 (1956), and J. Hetrocyclic Chem., 31, 1619 (1994).

Lactams can be produced by methods disclosed in Chem.

Pharm. Bull., 32(10), 4197-4204 (1984), Pharmazie, 43(7), 473-474 (1988), Monatsh. Chem., 123(1-2), 93-98 (1992), J.

Inorg. Biochem., 24(3), 167-181 (1985), J. Am. Chem. Soc., 107(18), 5219-5224 (1985), J. Org. Chem., 50(8), 1344-1346 (1985), J. Org. Chem., 58, 142-146 (1993), J. Org. Chem., 49, 3489 (1984), J. Hetrocycl. Chem., 19, 883 (1982), J.

Am. Chem. Soc., 72, 1236 (1950), J. Am. Chem. Soc., 72, 4447 (1950), J. Chem. Soc. 850 (1954), J. Chem. Soc., Perkin Trans. 1. 2907 (1973), Tetrahedron Lett., 25, 2009 (1984), Bull. Chem. Soc. Jpn., 59, 2185 (1986), Bull.

Chem. Soc. Jpn., 49, 1161 (1976), Chem. Pharm. Bull., 28, 2494 (1980), J. Am. Chem. Soc., 69, 2571 (1947), and Chem.

Pharm. Bull., 30(4), 1315 (1982). Thiotetronic acids can be produced by a method disclosed in J. Chem. Soc. 55 1501 (1968) or the like.

Acylmeldrum's acids can be produced by a method disclosed in J. Org. Chem., 43, 2087 (1978) or the like.

On clinical application, an agent containing an effective dose of a ketone of the present invention is administered orally or parenterally. Formulations of the agent include a tablet, a sugar-coated tablet, a pill, a capsule, a powder, a troche, a liquid, a suppository, or an injection solution, and they can be prepared by uniformly mixing with pharmaceutically acceptable excipients. As excipients, the following can be used: lactose, sucrose, glucose, sorbitol, mannitol, potato starch, amylopectin, other types of starches, cellulose derivatives carboxymethyl cellulose and hydroxyethyl cellulose), gelatin, magnesium stearate, polyvinyl alcohol, polyethylene glycol wax, gum arabic, talc, titanium dioxide, vegetable oils including olive oil, peanut oil, and sesame oil, paraffin-oils, neutral fat base, ethanol, propylene glycol, physiological saline, sterilized water, glycerine, coloring agents, flavoring materials, thickeners, stabilizing agents, isotonizing agents, buffers, and the like.

In the present invention, a hematopoietic agent means an agent which induces in vivo proliferation of platelets, white blood cells, red blood cells, and the like when 56 administered to humans or to animals so as to prevent or to treat cytopenia caused by cancer chemotherapy, radiation therapy, bone-marrow transplantation, drug therapy, immunological disorders, anemia such as renal anemia, hemorrhagic anemia, hemolytic anemia, and deficiency anemia. In addition, a hematopoietic agent of the present invention can also be applied to the field of treatment of leukopenia, idiopathic thrombocytopenic purpura, and the like caused by aplastic anemia, thrombocytopenia, infectious diseases, viral diseases, nutritional disorder, etc. Furthermore, it can be used for autologous blood storage.

For preventing or treating cytopenia caused by cancer chemotherapy, radiation therapy, bone-marrow transplantation, drug therapy, immunological disorders, and anemia such as renal anemia, hemorrhagic anemia, hemolytic anemia, and deficiency anemia, a hemopoietic agent of the present invention can be used in combination with an EPO, i. an erythrocytopoietic agent, or a G- CSF, i. a leukocytopoietic agent.

Although the dosage of the agent of the present invention varies according to the symptoms, body weight, age, and the route of administration, the general daily dose for an adult is preferably 0.01 mg to 2000 mg per day.

57 Examples The present invention will be further illustrated in detail with reference to the following comparative examples and examples.

[EXAMPLE 1] Synthesis of Compound 1 After 5.00 g (29.1 mmol) of L-cysteine methyl ester hydrochloride was heat-refluxed in 250 mL of acetone for 4 hours, the reaction solution was cooled in a refrigerator, and then, 5.57 g (26.3 mmol, 90.4%) of methyl 2,2dimethylthiazolidinedione 4-carboxylate hydrochloride was recovered by filtration.

1 H-NMR(300MHz, CDC1 3 1.86(3H, 1.87(3H, 3.59(1H, 3.72(1H, 3.94(3H, 5.11(1H, dd, J=8.71, 8.09) To a mixed solution of 4.00 g (18.9 mmol) of methyl 2,2-dimethylthiazolidinedione 4-carboxylate hydrochloride, mL of tetrahydrofuran, and 5.24 mL (37.8 mmol) of triethylamine, was added dropwise a solution of 1.44 mL (18.9 mmol) of diketene in 5 mL of tetrahydrofuran. After the addition of the diketene solution, the mixture was stirred at room temperature for 4 hours. Insoluble substances were removed by filtration and the filtrate was concentrated. To the residue, were added 5 mL of tetrahydrofuran and 25 mL of a 1.0 M tetrabutylammonium fluoride/tetrahydrofuran solution, followed by stirring at for 9 hours. The reaction solution was concentrated; 58 the residue was solubilized by adding water and extracted with dichloromethane. The resultant was dried over sodium sulfate anhydride and concentrated; the residue was purified by silica gel column chromatography (ethyl acetate:methanol 20:1) and recrystallized from ethyl acetate/diethyl ether. As a result, 2.14 g (mmol, of an orange tetrabutylammonium salt was obtained. In dichloromethane, was dissolved 2.14 g of the tetrabutylammonium salt, neutralized by adding 1 N hydrochloric acid, dried over sodium sulfate anhydride, and concentrated. The residue was purified by silica gel column chromatography (ethyl acetate:methanol 20:1) to obtain 445 mg (1.96 mmol, 10.3%) of the target compound in a free form.

[EXAMPLE 2] Synthesis of Compound 2 To a mixed solution of 1.50 g (6.21 mmol) of Lproline benzyl ester hydrochloride, 7.5 mL of tetrahydrofuran, and 1.72 mL (12.4 mmol) of triethylamine, was added dropwise a solution of 0.48 mL (6.21 mmol) of diketene in 1.5 mL of tetrahydrofuran, followed by stirring for 3 hours at room temperature. The reaction solution was filtered and the filtrate was concentrated.

The residue was purified by silica gel column chromatography (diethyl ether) to give 1.61 g (5.56 mmol, 89.5%) of a colorless oily substance.

59 In 5 mL of tetrahydrofuran, was dissolved 1.42 g (4.91 mmol) of the oily substance, and mixed with 5 mL of a 1.0 M-tetrabutylammonium fluoride/tetrahydrofuran solution, followed by stirring for 63 hours at room temperature. The reaction solution was concentrated; and then, the residue was purified by silica gel column chromatography (dichloromethane:methanol 10:1) to give 167 mg (0.92 mmol, 18.7%) of the target compound as an orange oily substance.

[EXAMPLE 3] Synthesis of Compound 3 To a mixed solution of 1.50 g (6.26 mmol) of Lglutamic acid diethyl ester hydrochloride, 9 mL of tetrahydrofuran, and 1.74 mL (12.6 mmol) of triethylamine, was added dropwise a solution of 0.48 mL (6.26 mmol) of diketene in 1.5 mL of tetrahydrofuran, followed by stirring for 3 hours at room temperature. The reaction solution was filtered and the filtrate was concentrated.

The residue was mixed with 6 mL of toluene and 4.1 mL of 2 N sodium methoxide/methanol and heat-refluxed for 3 hours.

The mixture was cooled to room temperature and concentrated; the residue was washed with acetone and filtered to recover a solid. The solid was dissolved in water, acidified by adding 2 N hydrochloric acid, and extracted with dichloromethane. The resultant was dried over sodium sulfate anhydride, filtered, and concentrated.

60 The residue was purified by silica gel column chromatography and the thus-obtained powder was washed with diethyl ether to give 205 mg (0.90 mmol, 14.4%) of the target compound.

[EXAMPLE 4] Synthesis of Compound 4 Similarly to Example 3, 1.75 g (7.20 mmol, 47.0%) of an acetamido derivative was obtained from 3.00 g (15.3 mmol) of L-leucine ethyl ester hydrochloride.

1 H-NMR(300MHz, CDC1 3 0.95(6H, m, J=3.30), 1.28(3H, t, J=7.14), 1.58-1.69(3H, 2.28(3H, 3.45(2H, s), 4.19(2H, q, J=7.14), 4.60(1H, 7.24(1H, br, NH) In 7.5 mL of benzene, was dissolved 1.75 g (7.20 mmol) of the acetamido derivative, and the resulting solution was mixed with 5 mL of 2 N sodium methoxide/methanol, followed by heat-refluxing for 2 hours. The reaction solution was cooled and concentrated.

The residue was dissolved in water, washed with diethyl ether, acidified by adding 2 N hydrochloric acid, and extracted with dichloromethane. The resultant was dried over sodium sulfate anhydride and concentrated to give 1.38 g of crude crystals. The crude crystals were recrystallized from ethyl acetate to give 228 mg (1.15 mmol, 1.16%) of colorless crystals.

[EXAMPLE 5] Synthesis of Compound To a mixed solution of 3.00 g (17.5 mmol) of L- 61 cysteine methyl ester hydrochloride, 20 mL of tetrahydrofuran, and 4.85 mL of triethylamine, was added dropwise 1.35 mL (17.6 mmol) of diketene, followed by stirring for 4 hours at room temperature. The reaction solution was filtered and the filtrate was concentrated.

The residue was purified by silica gel column chromatography (diethyl ether) to give 2.45 g (11.2 mmol, 64.0%) of a pale yellow oily substance.

1 H-NMR(300MHz, CDCl 3 2.30(3H, 3.02(2H, dd, J=4.39, 6.72), 3.50(2H, 3.80(3H, 4.89(1H, 7.43(1H, br,

NH)

In 20 mL of benzene, was dissolved 2.43 g (11.1 mmol) of the above oily substance, and then, 8 mL of 2 N sodium methoxide/methanol was added dropwise to the resulting solution, followed by heat-refluxing for 2 hours. After cooling the solution, the precipitate was recovered by filtration, dissolved in water, and washed with diethyl ether. The resultant was then acidified by adding concentrated hydrochloric acid for extraction with dichloromethane, however, it was not solubilized; thus the organic layer was recovered. The organic layer was concentrated; the residue was mixed with methanol, washed while being heated, and filtered for removing insoluble substances so as to give 475 mg (2.54 mmol, 22.9%) of the target compound as a pale yellow powder.

-62- [EXAMPLE 6] Synthesis of Compound 6 Similarly to Example 3, 2.57 g (<6.26 mmol, <100%) of an acetamido derivative was obtained from 3.01 g (6.20 mmol) of L-asparaginic acid dibenzyl ester.ptoluenesulfonate.

1 H-NMR(300MHz, CDC1 3 2.22(3H, 2.90(1H, dd, J=4.67, 17.03), 3.08(1H, dd, J=4.67, 17.03), 3.40(2H, 4.94(1H, 5.08(2H, 5.13(2H, 7.27-7.37(10H, 7.66(1H, d, J=8.24) IR(neat)3330, 1734, 1655, 1569, 1359, 741, 700 cm- 1 In 10 mL of benzene, was dissolved 2.57 g (<6.20 mmol) of the acetamido derivative, and the resulting solution was mixed with 2 N sodium methoxide/methanol (512 mg/3.1 mL), followed by heat-refluxing for 2 hours. The reaction solution was cooled and mixed with acetone so as to filter insoluble substances. The thus-obtained solid was dissolved in methanol and insoluble substances were removed by filtration. After concentrating the resultant, the residue was dissolved in water, acidified by adding 6 N hydrochloric acid, extracted with dichloromethane, and dried over sodium sulfate anhydride. The resultant was filtered, the filtrate was concentrated, and the residue was washed with diethyl ether to give 317 mg (1.48 mmol, 24.0%) of the target compound.

[EXAMPLE 7] Synthesis of Compound 7 63- Similarly to Example 3, 1.95 g (6.45 mmol, 65.5%) of an acetamido derivative was obtained from 2.51 g (9.85 mmol) of L-tryptophane methyl ester.hydrochloride.

1 H-NMR(300MHz, CDCl 3 2.16(3H, 3.32(2H, 3.33(2H, 3.69(3H, 4.92(1H, 7.05-7.36(4H, 7.54(1H, 8.22(1H, br) In 10 mL of benzene, was dissolved 1.95 g (6.45 mmol) of the acetamido derivative, and the resulting solution was mixed with 2 N sodium methoxide/methanol (520 mg/4.8 mL), followed by heat-refluxing for 2 hours. The reaction solution was cooled and concentrated. The residue was dissolved in water, acidified by adding 6 N hydrochloric acid, extracted with dichloromethane, and dried over sodium sulfate anhydride. The resultant was filtered, the filtrate was concentrated, the residue was purified by silica gel column chromatography (ethyl acetate), and the thus-obtained solid was recrystallized from ethyl acetate.

As a result, 312 mg (1.15 mmol, 17.9%) of the target compound was obtained.

[EXAMPLE 8] Synthesis of Compound 8 Sodium ethoxide was prepared from 35 mL of ethanol and 2.1 g (91.3 mmol) of sodium, and then, mixed with 10.7 g (93.0 mmol) of methyl 3-aminocrotonate and 14.2 mL (93.6 mmol) of dimethyl malonate, followed by heat-refluxing for hours. The reaction solution was cooled to room 64 temperature; the precipitated crystals were filtered and washed with ethanol. As a result, 5.27 g of a sodium salt of the target compound was obtained. The thus-obtained sodium salt was suspended in water, acidified by adding 3 N hydrochloric acid, filtered, washed with water, and recrystallized from ethanol. As a result, 1.63 g (8.26 mmol, of the target compound was obtained as colorless crystals.

[EXAMPLE 9] Synthesis of Compound 9 To 7.6 mL (109.3 mmol) of thioglycollic acid, was added dropwise 7.8 mL (109.7 mmol) of acetyl chloride while the mixture was stirred in an ice bath; After the addition of acetyl chloride, the mixture was heated to 0 C and stirred for one and half hours. To the reaction solution, was added 9.5 mL of thionyl chloride and stirred for further three and half hours. The reaction solution was concentrated, the residue was mixed with benzene and evaporated, which procedure was repeated three times, and then, the residue was distilled under reduced pressure to give 5.10 g (33.4 mmol, 30.5%) of a thioacetyl derivative.

In 65 mL of toluene, was dispersed 1.68 g (73.0 mmol) of metallic sodium and cooled to 5 0 C. To the mixture, 13.0 mL of ethyl acetoacetate was added dropwise while giving attention to temperature rise (5-10 0 and was stirred for three and half hours in an ice bath. To the mixture, 65 was further added dropwise 5.1 g (33.4 mmol) of the thioacetyl derivative, followed by stirring for one and half hours at 10-15*C. The reaction solution was subjected to extraction with 65 mL of 2 N hydrochloric acid, and then, with 10 w/v% sodium carbonate (20 mL x After adding 10.8 N-NaOH to the water layer, the resultant was allowed to stand for 3 days. The thus-precipitated crystals were filtered, dissolved in 80 mL of water, acidified by adding concentrated hydrochloric acid, and the thus-precipitated crystals were filtered. The thusobtained crystals were recrystallized from ethyl acetate to give 1.92 g (12.1 mmol, 36.2%) of pale yellow needle crystals.

[EXAMPLE 10] Synthesis of Compound To a mixed solution of 5.00 g (34.7 mmol) of meldrum's acid, 20 mL of dichloromethane, and 5.6 mL of pyridine, was added dropwise 5.2 mL (39.3 mmol) of phenacyl chloride while stirring the mixture in an ice bath. After the addition of phenacyl chloride, the mixture was poured in a separatory funnel, washed with 48 mL of 2 N hydrochloric acid, and dried over sodium sulfate anhydride. The resultant was filtered and concentrated; the residue was purified by silica gel column chromatography (diethyl ether). The resultant was recrystallized from diethyl ether to give 2.94 g (11.2 66mmol, 32.3%) of phenacylmeldrum's acid.

1 H-NMR(300MHz, CDC1 3 1.72(6H, 4.43(2H, s), 7.26-7.38(5H, m) In 10 mL of benzene, was dissolved 501 mg (1.92 mmol) of phenacylmeldrum's acid, and the resulting solution was mixed with 0.25 mL (2.18 mmol) of (S)-ethyl lactate, followed by stirring for 1.5 hours. The reaction solution was concentrated and the residue was purified by silica gel column chromatography (diethyl ether:hexane 1:3) to give 513 mg (1.84 mmol, 96%) of a colorless oily substance.

1H-NMR(300MHz, CDC1 3 1.28(3H, t, J=7.14), 1.49(3H, d, J=7.15), 3.53(2H, 3.88(2H, 4.21(2H, q, J=7.14), 5.11(1H, 7.21-7.35(5H, m) To 497 mg (1.78 mmol) of the colorless oily substance, was added 4 mL of 1 M-tetrabutylammonium fluoride, followed by stirring for 2 days at room temperature. The reaction solution was concentrated; the residue was mixed with water, acidified by adding 3 N hydrochloric acid, and then, extracted with dichloromethane. The resultant was dried over sodium sulfate anhydride, filtered, and concentrated. It was revealed from NMR measurement that the thus-obtained oily substance was a tetrabutylammonium salt. Thus, the oily substance was mixed with 6 mL of 1 N hydrochloric 67acid/ethanol, stirred, and concentrated; water was added to the residue for filtration. The thus-obtained powder was dissolved in dichloromethane and dried over sodium sulfate anhydride. After filtration and concentration, the residue was recrystallized from diethyl ether/hexane to give 196 mg (0.844 mmol, 47.4%) of the target compound as pale orange crystals.

[EXAMPLE 11] Synthesis of Compound 11 Similarly to Example 10, 799 mg (2.48 mmol, 92.5%) of a colorless oily substance was obtained from 700 mg (2.43 mmol) of phenacylmeldrum's acid and 0.385 mL (2.94 mmol) of DL-dimethyl malonate.

TH-NMR(300MHz, CDC13)6: 2.92(2H, d, J=6.04), 3.53(2H, s), 3.71(3H, d, J=3.02), 3.78(3H, d, J=3.30), 3.87(2H, s), 5.55(1H, 7.20-7.35(5H, m) IR(neat)2960, 1744, 1628, 1497, 1439, 1056, 702 cm- 1 Under similar conditions to Example 10, 783 mg (2.43 mmol) of the colorless oily substance was subjected to reaction and recrystallized from methanol to give 247 mg of pale orange crystals.

[EXAMPLE 12] Synthesis of Compound 12 In 5 mL of dichloromethane, was dissolved 705 mg (5.97 mmol) of monomethyl malonate, and mixed with 0.685 mL (5.97 mmol) of (S)-ethyl lactate; 0.94 mL (6.10 mmol) of diisopropylcarbodiimide was added dropwise to the 68 resultant mixture while stirring the mixture in an ice bath, the mixture was stirred for 9 hours in the ice bath, and then, allowed to stand at room temperature overnight.

After removing the precipitate by filtration, the filtrate was concentrated and the residue was purified by silica gel column chromatography (ethyl acetate:hexane 1:2) to give 1.19 g (5.45 mmol, 91.3%) of a colorless oily substance.

1 H-NMR(300MHz, CDCl 3 1.28(3H, t, J=7.14), 1.51(3H, d, J=7.14), 3.48(2H, 3.78(3H, 4.21(2H, q, J=7.14), 5.14(1H, q, J=7.14) IR(neat)2998, 2964, 1765, 1746, 1441, 1357, 1272, 1203, 1149, 1096, 1050, 1021 cm- 1 Under similar conditions to Example 10, 1.18 g (5.40 mmol) of the colorless oily substance was subjected to reaction to give 214 mg (1.24 mmol, 23.0%) of colorless crystals.

[EXAMPLE 13] Synthesis of Compound 13 Similarly to Example 12, 18.2 g (69.4 mmol, 91.7%) of a colorless oily substance was obtained from 10.0 g (84.7 mmol) of monomethyl malonate and 9.90 mL (75.7 mmol) of dimethyl malate.

IH-NMR(300MHz, CDC1 3 )6 0.93(2H, d, J=6.04), 3.48(2H, s), 3.73(3H, 3.76(3H, 3.79(3H, 5.55(1H, t, J=6.04) Under similar conditions to Example 10, 12.78 g (48.7 69 mmol) of the colorless oily substance was subjected to reaction and recrystallized from ethyl acetate to give 719 mg (3.12 mmol, of the target compound as colorless crystals.

[EXAMPLE 14] Synthesis of Compound 14 Similarly to Example 12, 2.45 g (9.20 mmol, 92.0%) of a colorless oily substance was obtained from 1.19 g (10.1 mmol) of monomethyl malonate and 1.66 g (10.0 mmol) of (S)-methyl mandelate.

1 H-NMR(300MHz, CDC13)6: 3.54(2H, 3.74(3H, 3.75(3H, 6.00(1H, 7.38-7.45(5H, m) Under similar conditions to Example 10, 2.43 g (9.12 mmol) of the colorless oily substance was subjected to reaction and recrystallized from ethyl acetate/methanol to give 895 mg (3.82 mmol, 41.9%) of colorless crystals.

[EXAMPLE 15] Synthesis of Compound To 5.57 g (37.8 mmol) of L-glutamic acid, was added 100 mL of a 20% aqueous acetic acid solution, and a 3.45 g (50.0 mmol)/100ml aqueous sodium nitrite solution was slowly added dropwise to the'resultant mixture while stirring the mixture in an ice bath. After the addition of the aqueous sodium nitrite solution, the mixture was stirred in the ice bath for 1 hour, removed from the ice bath, and further stirred at room temperature overnight without the ice bath. The reaction solution was 70 concentrated and dried to a solid by a vacuum pump. To the residue, was added 50 mL of methanol, and the mixture was heat-refluxed for 5 hours in gaseous hydrochloric acid. After removing methanol by evaporation, 130 mL of methanol was further added, followed by heat-refluxing for hours in gaseous hydrochloric acid. After removing methanol by evaporation, the residue was distilled (0.06 mmHg, 72-73°C) to give 3.97 g (22.5 mmol, 59.5%) of dimethyl 2-hydroxyglutarate.

1H-NMR(300MHz, CDC1 3 1.95(1H, 2.17(1H, 2.48(2H, 3.69(3H, 3.80(3H, 4.25(1H, m) To 1.97 g (11.2 mmol) of dimethyl-2-hydroxyglutarate, were added 10 mL of tetrahydrofuran and 0.1 mL of pyridine, and then, while stirring the mixture at 60*C, 0.86 mL (11.2 mmol) of diketene and 2 mL of tetrahydrofuran were added dropwise to the mixture, followed by stirring at 60°C for 4 hours.

The reaction solution was concentrated, and the residue was purified by silica gel column chromatography to give 2.67 g (10.2 mmol, 91.1%) of an acetoacetate derivative.

1 H-NMR(300MHz, CDC1 3 2.12-2.29(2H, 2.31(3H, s), 2.46(2H, 3.53(2H, 3.69(3H, 3.77(3H, s), 5.14(1H, m).

To 2.67 g (10.2 mmol) of the acetoacetate derivative, was added 11 mL of 1 M-tetrabutylammonium fluoride, and the mixture was stirred at room temperature overnight; the 71 resultant was subjected to reaction under similar conditions to Example 10 to give 759 mg (3.33 mmol, 32.6%) of a white solid.

[EXAMPLE 16] Synthesis of Compound 16 The water layer of the compound 13 was allowed to stand overnight, and a white precipitate was filtered.

[EXAMPLE 17] Synthesis of Compound 17 In 50 mL of dichloromethane, was dissolved 5.00 g (42.3 mmol) of monomethyl malonate, and mixed with 4.85 mL (42.3 mmol) of methyl 2-hydroxyglutarate; 6.65 mL (43.2 mmol) of diisopropylcarbodiimide was added dropwise to the resulting mixture while stirring the mixture in an ice bath, the mixture was stirred in the ice bath for hours, and then, stirred at room temperature overnight.

After removing the precipitate by filtration, the filtrate was concentrated'and the residue was purified by silica gel column chromatography (ethyl acetate:hexane 1:3) to give 7.93 g (36.3 mmol, 85.8%) of a monomethyl malonate derivative as a colorless liquid.

1 H-NMR(300MHz, CDC1 3 1.58(6H, 3.40(2H, 3.74(3H, 3.76(3H, s) IR(neat)3000, 2960, 1744, 1439, 1131, 1023 cm- 1 Under similar conditions to Example 10, 7.92 g (36.3 mmol) of the monomethyl malonate derivative was subjected to reaction to give 942 mg (5.06 mmol, 13.9%) of a 72 colorless powder.

[EXAMPLE 18] Synthesis of Compound 18 To 5.02 g (30.2 mmol) of L (-)-3-phenylacetic acid, was added 50 mL of 1 N hydrochloric acid/ethanol, followed by heat-refluxing for 5 hours. The reaction solution was evaporated and the residue was purified by distillation under reduced pressure (0.01 mmHg, 79-81 0 C) to give 4.26 g (21.9 mmol, 72.5%) of ethyl (-)-3-phenylacetate.

1 H-NMR(300MHz, CDCl 3 1.28(3H, t, J=7.14), 2.97(1H, dd, J=6.87, 14.00), 3.13(1H, dd, J=4.67, 14.00), 4.22(2H, q, J=7.14), 4.43(1H, dd, J=4.70, 6.87), 7.21-7.32(5H, m) To 1.00 g (5.15 mmol) of ethyl (-)-3-phenylacetate, were added 5 mL of tetrahydrofuran and 0.1 mL of pyridine, and then, while stirring the resulting mixture at 60°C, a solution of 0.4 mL (5.23 mmol) of diketene in 2 mL of tetrahydrofuran was added dropwise to the mixture. After the addition of the diketene solution, the resulting mixture was stirred at 60 0 C for 4 hours and the reaction solution was evaporated. The residue was purified by silica gel column chromatography (ethyl acetate:hexane 1:2) to give 1.37 g (4.92 mmol, 95.5%) of a colorless oily acetoacetate derivative.

'H-NMR(300MHz, CDC13)6: 1.24(3H, t, J=7.14), 2.18(3H, s), 3.09-3.20(2H, 3.45(2H, 4.19(2H, q, J=7.14), 5.27(1H, dd, J=4.70, 8.78), 7.19-7.30(5H, m) 73 IR(neat)1746, 1721, 1195, 1151, 1071, 1033, 702 cm- 1 In 5 mL of tetrahydrofuran, was dissolved 1.34 g (4.81 mmol) of the acetoacetate derivative, and then, the resulting solution was subjected to reaction under similar conditions to Example 10 to give 260 mg (1.12 mmol, 23.3%) of the target compound.

[EXAMPLE 19] Synthesis of Compound 19 In 10 mL of dichloromethane, was dissolved 1.01 g (8.55 mmol) of 1-hydroxycinnamic acid, and mixed with 1.66 g (8.54 mmol) of monomethyl malonate; 1.34 mL (8.70 mmol) of diisopropylcarbodiimide was added dropwise to the resulting mixture while stirring the mixture in an ice bath, the mixture was stirred in the ice bath for 10 min., removed from the ice bath, and further stirred overnight.

After removing insoluble substance by filtration, the filtrate was concentrated. The residue was purified by silica gel column chromatography (ethyl acetate:hexane 1:3) to give 2.52 g (8.56 mmol, 100%) of a malonic acid derivative.

1 H-NMR(300MHz, CDC13)6: 1.23(3H, t, J=7.14), 3.16(2H, m), 3.42(2H, 3.69(3H, 4.18(2H, q, J=7.14), 7.19-7.33(5H, m) IR(neat)1742, 1456, 1439, 1280, 1195, 1152, 1031, 702 cm- 1 Under similar conditions to Example 10, 2.50 g (8.49 mmol) of the malonic acid derivative was subjected to 74 reaction and recrystallized form ethyl acetate to give 527 mg (2.12 mmol, 25.0%) of colorless crystals.

[EXAMPLE 20] Synthesis of Compound To 1.21 g (4.99 mmol) of methyl benzilate, were added mL of tetrahydrofuran and 0.1 mL of pyridine, and then, while stirring the mixture at 50°C, 0.4 mL (5.23 mmol) of diketene was added dropwise to the mixture, followed by stirring at 50 0 C for 7 hours. The reaction solution was concentrated and the residue was purified by silica gel column chromatography (ethyl acetate:hexane 1:4) to give 1.39 g (4.26 mmol, 85.4%) of an acetoacetic acid derivative.

1 H-NMR(300MHz, CDC1 3 2.22(3H, 3.53(2H, 3.76(3H, 3.53(2H, 7.30-7.33(6H, 7.49-7.53(4H, m) IR(KBr )1760, 1744, 1725, 1315, 1267, 1143, 69 cm- 1 Under similar conditions to Example 10, 1.26 g (3.86 mmol) of the acetoacetic acid derivative was subjected to reaction to give 865 mg (2.94 mmol, 76.1%) of a pale yellow powder.

[EXAMPLE 21] Synthesis of Compound 21 In ether, was suspended 974 mg (4.86 mmol) of DL-3- (4-hydroxyphenyl)lactic acid monohydrate, and then, diazomethane was added to the resulting suspension until the bubbling stopped. After removing insoluble substances by filtration, the resultant was condensed, and the 75 residue was purified by silica gel column chromatography (ethyl acetate:hexane 1:2) to give 977 mg (4.86 mmol, 100%) of a methyl ester compound.

1 H-NMR( 300MHz, CDCl 3 )8: 2.74(lH,d,J=6.32),2.90(lH,dd,J=6.59,14.00),3.6(H,dd,J=4.

39,14.00),3.78(3H,s),4,43(lH,m),5.08(1H,s),6.72-6.76(21,m) 7.05-7.09(2H,m) IR(neat): 3400,l734,1615,1518,1446,1224,1108,828,803cm-' To a mixed solution of 449 mg (2.28 mmol) of the methyl ester compound, 3 mL of tetrahydrofuran, and 0.05 mL of pyridine, was added 0.2 mL of diketene while stirring the mixture at 60 0 C, followed by stirring for 4 hours. The reaction solution was concentrated, and the residu e was purified by silica gel column chromatography to give 534 mg (1.90 mrnol, 83.3%) of an acetoacetic acid derivative.

NMR( 300MHz, CDC1 3 )6: 2.20(3H,S),3.04(1H,dd,J=8.24,14.00),3.14(lH,dd,J=4.39,l 4

.O

0),3.46(2H,d,J=0.55),3.74(3H,s),5.25(lH,dd,J=4.67,8.24),6.

74-6.77(2H,m),7.04-7.07(2H,m) IR(neat): 3416,1748,1734,1717,1518,1228cm- 1 Under similar conditions to Example 10, 520 mg (1.85 mmol) of an acetamidoacetic acid derivative was subjected to reaction to give 225 mg (0.906 mmol, 49.0%) of the target compound as a colorless powder.

76 [EXAMPLE 22] Synthesis of Compound 22 To a mixed solution of 510 mg (2.60 mmol) of methyl l-hydroxy-p-hydroxycinnamate, 339 mg (2.87 mmol) of monomethyl malonate, and 4 mL of dichloromethane, was added 0.5 mL of diisopropylcarbodiimide while stirring the mixture in an ice bath; the mixture was stirred in the ice bath for 10 min., removed from the ice bath, and stirred at room temperature for 1 day. Insoluble substances were filtered and washed with ethyl acetate. The mother liquor was concentrated, and the residue was purified by silica gel column chromatography (ethyl acetate:hexane 1:2) to give 631 mg of a mixed compound containing a malonic acid derivative. Under similar conditions to Example 10, the mixed compound was subjected to reaction to give 188 mg (0.711 mmol, 27.3%) of the target compound as a colorless powder.

77 [EXAMPLE 23] Synthesis of Compound 23 To 25.4 g (approximately 95.8 mmol) of 40% glyceric acid water, were added 200 mL of ethanol and 100 mL of 1 N hydrochloric acid/ethanol, followed by heat-refluxing for 2 hours. The mixture was cooled to room temperature, concentrated, mixed with 200 mL of ethanol and 25 mL of 1 N hydrochloric acid/ethanol, and heat-refluxed for 2 hours, which procedure was repeated twice; the reaction solution was concentrated, mixed with 200 mL of ethanol and 100 mL of 1 N hydrochloric acid/ethanol, and heatrefluxed for 5 hours. The reaction solution was concentrated and dried to a solid under reduced pressure to give 15.98 g (119 mmol) of an ethyl ester compound. To the ethyl ester compound, were added 20 mL of dichloromethane and 1.55 mL of pyridine, and then, while stirring the mixture in an ice bath, 9.0 mL (95.4 mmol) of acetic acid anhydride was added dropwise to the mixture.

The mixture was stirred for 3 hours in the ice bath, removed from the ice bath, and stirred at room temperature overnight. Insoluble substances were removed by filtration; the mother liquor was mixed with dichloromethane and washed with diluted hydrochloric acid.

The dichloromethane layer was dried over sodium sulfate anhydride and concentrated. The residue was purified by silica gel column chromatography (ethyl acetate:hexane 78 1:1) to give 7.21 g (45.0 mmol, 47.0%) of a monoacetyl compound.

1H-NMR(300MHz, CDC1 3 1.29(3H, td, J=5.49, 6.32), 4.23-4.46(6H, m) A mixed solution of 721 mg (4.50 mmol) of the monoacetyl compound, 1.07 g (4.10 mmol) of phenylmeldrum's acid, and 20 mL of benzene was heat-refluxed for 3 hours.

The reaction solution was cooled to room temperature and concentrated; the residue was purified by silica gel column chromatography (ethyl acetate:hexane 1:4) to give 1.04 g (75.4 mmol) of an acetoacetic acid derivative.

'H-NMR(300MHz, CDCl 3 1.29(3H, t, J=6.32), 2.07(3H, s), 3.57(2H, 3.88(2H, 4.25(2H, q, J=6.32), 4.45(2H, d, J=4.39), 5.35(1H, 7.21-7.35(5H, m) IR(neat): 1748, 1373, 1216, 1096, 702 cm- 1 Under similar conditions to Example 10, 1.02 g (3.03 mmol) of the acetoacetic acid derivative was subjected to reaction to give 352 mg (1.21 mmol, 39.3%) of the target compound as a colorless powder.

[EXAMPLE 24] Synthesis of Compound 24 In a mixed solution of 5.00 g (34.7 mmol) of meldrum's acid, 20 mL of dichloromethane, and 5.6 mL of pyridine, was added dropwise 5.8 mL (39.0 mmol) of hydrocinnamyl chloride while stirring the mixed solution in an ice bath. After the addition of hydrocinnamyl 79 chloride, the mixture was stirred for 1 hour in the ice bath and for 6 hours at room temperature. The reaction solution was mixed with 6 N hydrochloric acid and extracted with dichloromethane. The resultant was dried over sodium sulfate anhydride, filtered, and concentrated; the residue was purified by silica gel column chromatography (ethyl acetate:hexane 1:20) to give 6.68 g (24.1 mmol, 69.4%) of pale yellow oily acylmeldrum's acid.

1 H-NMR(300MHz, CDC1 3 1.67(s, 6H), 3.02(m, 2H), 3.41(m, 2H), 7.20-7.30(m, 5H)1154, 1031, 926, 700 cm- 1 A mixed solution of 3.11 g (11.2 mmol) of acylmeldrum's acid, 45 mL of benzene, 1.55 mL (13.5 mmol) of (S)-ethyl lactate was heat-refluxed for 4 hours. The reaction solution was concentrated and the residue was purified by silica gel column chromatography (ethyl acetate:hexane 1:3) to give 3.02 g (10.3 mmol, 92.0%) of a colorless oily substance.

'H-NMR(300MHz, CDC1l 3 1.26(t, J=7.14, 3H), 1.48(d, J=7.14, 3H), 2.91-3.02(m, 4H), 3.61(s, 2H), 4.22(q, J=7.14, 2H), 5.08(m, 1H), 7.19-7.32(m, IR(neat)1748, 1721, 1456, 1212, 1096, 700 cm- 1 Under similar conditions to Example 10, 3.01 g (10.3 mmol) of the colorless oily substance was subjected to reaction and recrystallized from ethyl acetate/hexane to 80 give 1.26 g (5.11 mmol, 49.6%) of colorless crystals.

[EXAMPLE 25] Synthesis of Compound In a mixed solution of 3.37 g (12.2 mmol) of 3phenylpropionylmeldrum's acid, 45 mL of benzene, and 1.92 mL (14.6 mmol) of dimethyl malate was heat-refluxed for 4 hours. The reaction solution was concentrated and the residue was purified by silica gel column chromatography (ethyl acetate:hexane 1:4) to give 3.04 g (9.04 mmol, 74.1%) of a colorless oily substance.

1 H-NMR(300MHz, CDC1,)&: 2.89-2.92(m, 6H), 3.50(s, 2H), 3.70, 3.72(s, s, 3H), 3.76, 3.79(s, s, 3H), 5.54(t, J=6.31, 1H), 7.17-7.28(m, IR(neat)1748, 1439, 1286, 1176, 1058, 702 cm- 1 Under similar conditions to Example 10, 3.01 g (10.3 mmol) of the colorless oily substance was subjected to reaction and recrystallized from ethyl acetate/hexane to give 1.26 g (5.11 mmol, 49.6%) of colorless crystals.

[EXAMPLE 26] Synthesis of Compound 26 To a mixed solution of 5.00 g (34.7 mmol) of meldrum's acid, 20 mL of dichloromethane, and 5.6 mL of pyridine, was added dropwise 4.8 mL (39.0 mmol) of 2thiopheneacetyl chloride while stirring the mixed solution in an ice bath. After the addition of 2-thiopheneacetyl chloride, the mixture was stirred for 1 hour in the ice bath and for 4 hours at room temperature. The reaction 81 solution was mixed with 2 N hydrochloric acid and extracted with dichloromethane. The resultant was dried over sodium sulfate anhydride, filtered, and concentrated; the residue was purified by silica gel column chromatography (ethyl acetate:hexane 1:20) to give 3.61 g (13.4 mmol, 38.6%) of acylmeldrum's acid as a pale brown oily substance.

iH-NMR(300MHz, CDCl 3 1.74(s, 6H), 4.61(s, 2H), 6.97(dd, J=3.57, 5.21, 1H), 7.07(m, 1H), 7.23(dd, J=1.37, 5.21, 1H) IR(neat)1744, 1676, 1659, 1572, 1410, 1270, 1201, 1025, 934, 704 cm- 1 A mixture of 3.60 g (13.4 mmol) of acylmeldrum's acid, 45 mL of benzene, 1.76 mL (13.4 mmol) of dimethyl malate was heat-refluxed for 2 hours. The reaction solution was concentrated and the residue was purified by silica gel column chromatography (ethyl acetate:hexane 1:4) to give 3.87 g (11.8 mmol, 88.0%) of a colorless oily substance.

1H-NMR(300MHz, CDCl 3 2.92(d, J=6.04, 2H), 3.57(s, 2H), 3.71, 3.72(s, s, 3H), 3.78, 3.79(s, s, 3H), 4.08(s, 2H), 5.55(t, J=6.04, 1H), 6.93(m, 1H), 6.99(m, 1H), 7.25(m, 1H) IR(neat)1767-1717, 1437, 1054, 853, 1096, 704 cm- 1 Under similar conditions to Example 10, 3.86 g (11.7 mmol) of the colorless oily substance was subjected to reaction and recrystallized from ethyl acetate to give 82 1.17 g (3.95 mmol, 33.8%) of pale brown crystals.

[EXAMPLE 27] Synthesis of Compound 27 Similar to Example 26, 3.07 g (14.3 mmol, 20.6%) of colorless oily acylmeldrum's acid was obtained from 10.0 g (69.4 mmol) of meldrum's acid and 8.8 mL (83.4 mmol) of isobutyryl chloride.

1 H-NMR(300MHz, CDC1 3 .1.24(d, J=6.86, 6H), 1.74(s, 6H), 4.09(m, 1H), 7.07(m, 1H), 7.23(dd, J=1.37, 5.21, 1H) IR(neat)1744, 1667, 1576, 1421, 1332, 1207, 1021, 953, 922 cm- 1 A mixture of 3.06 g (14.2 mmol) of acylmeldrum's acid, 40 mL of benzene, and 2.1 mL (16.0 mmol) of dimethyl malate was heat-refluxed for 4 hours. The reaction solution was concentrated and the residue was purified by silica gel column chromatography (ethyl acetate:hexane 1:4) to give 3.05 g (11.1 mmol, 78.1%) of a colorless oily substance.

'H-NMR(300MHz, CDC1 3 1.14(d, J=6.86, 6H), 2.76(m, 1H), 2.92(d, J=6.04, 2H), 3.58(s, 2H), 3.72, 3.73(s, s, 3H), 3.78, 3.79(s, s, 3H), 5.54(t, J=6.04, 1H), IR(neat)1750, 1717, 1216, 1174, 1046 cm- 1 Under similar conditions to Example 10, 3.04 g (11.0 mmol) of the colorless oily substance was subjected to reaction to give 2.33 g (9.62 mmol, 87.4%) of a pale orange oily substance.

83 [EXAMPLE 28] Synthesis of Compound 28 In 50 mL of ether, was dissolved 2.50 g (15.8 mmol) of hexahydromandelic acid, and then, a solution of diazomethane in ether was added to the resulting solution until the bubbling stopped, followed by concentration.

The residue was mixed with 1.87 g (15.8 mmol) of monomethyl malonate and 20 mL of dichloromethane, and then, 3.75 mL of N,N'-diisopropylcarbodiimide was added to the mixture while stirring the mixture in an ice bath, followed by stirring at room temperature overnight. The reaction solution was filtered, washed with ethyl acetate, and concentrated. The residue was purified by silica gel column chromatography (ethyl acetate:hexane 1:4) to give 4.13 g (15.1 mmol, 95.5%) of a colorless oily substance.

iH-NMR(300MHz, CDC1 3 1.01-1.91(m, 11H), 3.49(d, J=3.84, 2H), 3.75(s, 3H), 3.77(s, 3H), 4.90(d, J=4.94, 1H) IR(neat)1744, 1439, 1272, 1210, 1149, 1023 cm-' Under similar conditions to Example 10, 4.12 g (15.1 mmol) of the colorless oily substance was subjected to reaction and recrystallized from ethyl acetate to give 1.18 g (4.91 mmol, 32.5%) of colorless crystals.

[EXAMPLE 29] Synthesis of Compound 29 In 50 mL of ether, 2.50 g (15.8 mmol) of hexahydromandelic acid was dissolved, and then, a solution of diazomethane in ether was added to the resulting 84 solution until the bubbling stopped, followed by concentration. To the residue, were added 15 mL of tetrahydrofuran and 0.2 mL of pyridine, and then, 1.27 mL (16.6 mmol) of diketene was added dropwise to the mixture over 20 min. while stirring the mixture at 60. After stirring for 4 hours at 60, the reaction solution was concentrated. The residue was purified by silica gel column chromatography (ethyl acetate:hexane 1:5) to give 3.90 g (15.2 mmol, 96.2%) of a colorless oily substance.

1 H-NMR(300MHz, CDCl 3 1.13-1.95(m, 11H), 2.32(s, 3H), 3.53(s, 2H), 3.75(s, 3H), 4.89(d, J=4.67, 1H) IR(neat)2932, 2860, 1744, 1723, 1452, 1363, 1152, 1106, 1017 cm- 1 Under similar conditions to Example 10, 3.89 g (15.1 mmol) of the colorless oily substance was subjected to reaction and recrystallized from ethyl acetate to give 1.00 g (4.46 mmol, 29.5%) of pale yellow crystals.

[EXAMPLE 30] Synthesis of Compound To 10.00 g (58.6 mmol) of 2-chlorophenylacetic acid, was added 100 mL of thionyl chloride, and the mixture was heat-refluxed for 4 hours. After removing excessive thionyl chloride by distillation, the residue was distilled under reduced pressure to give 8.91 g of a pale red liquid.

Similar to Example 26, 8.27 g of colorless 85 oily acylmeldrum's acid was obtained from 6.71 g (mmol) of meldrum's acid and 8.8 g (46.6 mmol) of 2-chlorophenacyl chloride.

1 H-NMR(300MHz, CDC1 3 1.77(s, 6H), 4.61(s, 2H), 7.24-7.29(m, 3H), 7.39-7.43(m, 1H) A mixed solution of 3.00 g (10.1 mmol) of acylmeldrum's acid, 40 mL of benzene, and 1.32 mL (10.1 mmol) of dimethyl malate was heat-refluxed for 5 hours.

The reaction solution was concentrated and the residue was purified by silica gel column chromatography (ethyl acetate:hexane 1:3) to give 3.56 g of a colorless oily substance.

1 H-NMR(300MHz, CDCl 3 2.93(d, J=6.046, 2H), 3.60(s, 2H), 3.72(s, 3H), 3.79(s, 3H), 4.02(s, 2H), 5.56(t, J=6.04, 1H), 7.22-7.33(m, 3H), 7.37-7.41(m, 1H) Under similar conditions to Example 10, 3.55 g (9.96 mmol) of the colorless oily substance was subjected to reaction and recrystallized from ethyl acetate to give 2.41 g of colorless crystals.

[EXAMPLE 31] Synthesis of Compound 31 In 40 mL of dichloromethane, was dissolved 10.00 g (69.4 mmol) of meldrum's acid, and the resulting solution was mixed with 11.2 mL of pyridine, and then, 9.6 mL (69.4 mmol') of monomethyl glutarate was added dropwise to the mixture while stirring the mixture in an ice bath. After 86the addition of monomethyl glutarate, the mixture was stirred for 1 hour in the ice bath, removed from the ice bath, and stirred for further 24 hours. The reaction solution was mixed with 75 mL of 2 N hydrochloric acid, extracted with dichloromethane, and dried over sodium sulfate anhydride. After being concentrated under reduced pressure, the residue was purified twice by silica gel column chromatography (ethyl acetate:hexane 1:9) to give 9.71 g (35.7 mmol, 51.3%) of acylmeldrum's acid as a yellow oily substance.

TH-NMR(300MHz, CDC1 3 1.74(d, J=6.87, 6H), 2.05(quiNt, J=7.14, 2H), 2.44(t, J=7.14, 2H), 3.15(t, J=7.14, 2H), 3.69(s, 3H), IR(neat)1736, 1669, 1576, 1274, 1205, 1156, 1029, 924 cm- 1 A mixed solution of 3.00 g (11.0 mmol) of acylmeldrum's acid, 40 mL of benzene, and 1.44 mL (11.0 mmol) of DL-dimethyl malate was heat-refluxed for 5 hours.

The reaction solution was concentrated and the residue was purified by silica gel column chromatography (ethyl acetate:hexane 1:3) to give 3.06 g (9.20 mmol, 83.6%) of a colorless oily p-ketoester.

1 H-NMR(300MHz, CDC1 3 1.92(q,J=7.14, 2H), 2.36(t, J=7.14, 2H), 2.67(t, J=7.14, 2H), 2.92(d, J=6.04, 2H), 3.51(s, 2H), 3.67(s, 3H), 3.73(s, 3H), 3.78(s, 3H), 5.54(s, 1H) 87 IR(neat)1744, 1439, 1176, 1058 cm-' Under similar conditions to Example 10, 3.06 g (9.20 mmol) of the p-ketoester was subjected to reaction to give 1.68 g (5.60 mmol, 60.8%) of an orange oily substance.

[EXAMPLE 32] Synthesis of Compound 32 A mixed solution of 3.01 g (11.0 mmol) of 3carbomethoxybutyric meldrum's acid, 40 mL of benzene, and 1.83 g (11.0 mmol) of (S)-methyl mandelate was heatrefluxed for 5 hours. The reaction solution was concentrated and the residue was purified by silica gel column chromatography (ethyl acetate:hexane 1:3) to give 3.63 g of a colorless solid p-ketoester.

'H-NMR(300MHz, CDC1 3 1.93(q, J=7.14, 2H), 2.35(t, J=7.14, 2H), 2.69(t, J=7.14, 2H), 3.57(d, J=0.82, 2H), 3.66(s, 3H), 3.73(s, 3H), 5.97(s, 1H), 7.38-7.45(m, IR(KBr)1734, 1719, 1222, 1180, 1042, 733, 698 cm- 1 Under similar conditions to Example 10, 3.62 g (10.7 mmol) of the p-ketoester was subjected to reaction and recrystallized from methanol to give 1.60 g of colorless crystals.

[EXAMPLE 33] Synthesis of Compound 33 A mixed solution of 2.29 g (8.76 mmol) of phenylacetylmeldrum's acid, 45 mL of benzene, and 1.48 mL 88 (8.79 mmol) of DL-diethyl malate was heat-refluxed for hours. The reaction solution was concentrated and the residue was purified by silica gel column chromatography (ethyl acetate:hexane 1:3) to give 2.34 g (6.68 mmol, 76.2%) of a colorless oily p-ketoester.

1 H-NMR(300MHz, CDC1 3 1.23-1.30(m, 6H), 2.89(d, J=6.04, 2H), 35.3(s, 2H), 3.88(s, 3H), 4.11-4.25(m, 4H), 5.53(t, J=6.04, 1H), 7.20-7.35(m, IR(neat)1738, 1377, 1183, 1054, 1029, 702 cm- 1 Under similar conditions to Example 10, 2.33 g (6.65 mmol) of the p-ketoester was subjected to reaction and recrystallized from methanol to give 1.35 g of colorless crystals.

[EXAMPLE 34] Synthesis of Compound 34 A mixed solution of 3.87 g (14.8 mmol) of phenylacetylmeldrum's acid, 70 mL of benzene, and 3.95 g (22.2 mmol) of (L)-(+)-dimethyl tartrate was heat-refluxed for 5 hours. The reaction solution was concentrated and the residue was purified by silica gel column chromatography (ethyl acetate:hexane 1:3 to 1:1) to give 4.12 g of a colorless oily substance. It was supposed from the results of NMR that the substance was a mixed compound.

Under similar conditions to Example 10, 4.12 g of the oily substance was subjected to reaction and 89 recrystallized from methanol to give 1.51 g (4.93 mmol, 33.3%) of colorless crystals.

[EXAMPLE 35] Synthesis of Compound In 8 mL of ethanol, was dissolved 834 mg (2.78 mmol) of 3-(4-methoxycarbonyl)butyryl-5methoxycarbonylmethyltetronic acid, and then, mixed with 83 mg of 5% Pd-C (containing water), followed by stirring for 4 hours in a hydrogen atmosphere. Since the result of thin-layer chromatography was not different from that of the raw material, 158 mg of 10% Pd-C (containing water) was added to the resultant, followed by stirring for 3 hours in a hydrogen atmosphere. Although the result of thin-layer chromatography was not different from that of the raw material, the catalyst was removed by filtration and the filtrate was concentrated under reduced pressure.

The residue was purified by silica gel column chromatography (ethyl acetate:hexane 1:1) to give 365 mg of colorless crystals. (1.27 mmol, 45.7%) [EXAMPLE 36] Synthesis of Compound 36 A mixed solution of 2.87 g (10.9 mmol) of phenylacetylmeldrum's acid, 40 mL of benzene, and 1.94 g (11.0 mmol) of dimethyl 2-hydroxyglutarate was heatrefluxed for 5 hours. The reaction solution was concentrated and the residue was purified by middlepressure column chromatography (silica gel, ethyl 90 acetate:hexane 1:3) to give 2.91 g (8.65 mmol, 79.3%) of an orange oily p-ketoester.

1 H-NMR(300MHz, CDC1 3 2.15-2.24(m, 2H), 2.44(m, 2H), 3.54(s, 2H), 3.69(s, 3H), 3.76(s, 3H), 3.87(s, 2H), 5.13(dd, J=4.67, 7.96, 1H), 7.21-7.36(m, IR(neat)1744, 1439, 1212, 1077, 702 cm- 1 Under similar conditions to Example 10, 2.91 g (8.65 mmol) of the P-ketoester was subjected to reaction and recrystallized from methanol to give 1.52 g (4.99 mmol, 57.7%) of colorless crystals.

[EXAMPLE 37] Synthesis of Compound 37 In 27 mL of ethanol, was dissolved 1.27 g (4.38 mmol) of 5-methoxycarbonylmethyl-3-phenacyltetronic acid, and then, mixed with 254 mg of 5% Pd-C (containing water), followed by stirring for 20 hours in a hydrogen atmosphere. After removing the catalyst by filtration, the mother liquor was concentrated. The residue was recrystallized from ethyl acetate to give 785 mg (2.84 mmol, 65.0%) of colorless crystals.

[EXAMPLE 38] Synthesis of Compound 38 A mixed solution of 3.00 g (20.8 mmol) of meldrum's acid, 257 mg (2.10 mmol) of 4-dimethylaminopyridine, 3.55 g (20.8 mmol) of 4-chlorophenylacetic acid, 4.72 g (22.8 mmol) of dicyclohexylcarbodiimide, and 50 mL of dichloromethane was stirred for 30 min. in an ice bath, 91 removed from the ice bath, and stirred.for further 2 days.

After removing insoluble substances by filtration, the mother liquor was concentrated under reduced pressure, and the residue was purified by silica gel column chromatography (ethyl acetate:hexane 1:9) to give 1.73 g (5.83 mmol, 28.0%) of acylmeldrum's acid as colorless crystals.

1 H-NMR(300MHz, CDC1 3 1.72(s, 6H), 4.38(s, 2H), 7.31-7.33(m, 4H) IR(neat)1744, 1651, 1593, 1427, 1383, 1319, 1154, 1019 cm 1 A mixed solution of 1.72 g (5.80 mmol) of acylmeldrum's acid, 23 mL of benzene, and 0.77 mL (5.88 mmol) of DL-dimethyl malate was heat-refluxed for 5 hours.

The reaction solution was concentrated and the residue was purified by silica gel column chromatography (ethyl acetate:hexane 1:3) to give 2.00 g (5.60 mmol, 96.5%) of a colorless oily p-ketoester.

1H-NMR(300MHz, CDC1 3 2.92(dd, J=6.04, 7.96, 2H), 3.54(s, 2H), 3.72(s, 3H), 3.79(s, 3H), 3.87(s, 2H), 5.55(m, 1H), 7.13-7.20(m, 2H), 7.29-7.33(m, 2H) IR(neat)1746, 1493, 1439, 1199, 1176, 1091, 1056 cm- 1 Under similar conditions to Example 10, 1.99 g (5.58 mmol) of the p-ketoester was subjected to reaction and recrystallized from methanol to give 1.51 g of 92 colorless crystals.

[EXAMPLE 39] Synthesis of Compound 39 A mixed solution of 3.30 g (22.9 mmol) of meldrum's acid, 279 mg (2.29 mmol) of 4-dimethylaminopyridine, 3.80 g of 4-methoxyphenylacetic acid, 5.20 g (25.2 mmol) of dicyclohexylcarbodiimide, and 50 mL of dichloromethane was stirred for 30 min. in an ice bath, removed from the ice bath, and stirred for further 2 days. After removing insoluble substances by filtration, the mother liquor was concentrated under reduced pressure, and the residue was purified by silica gel column chromatography (ethyl acetate:hexane 1:9) to give 2.23 g (7.63 mmol, 33.3%) of acylmeldrum's acid as colorless crystals.

'H-NMR(300MHz, CDCl 3 1.72(s, 6H), 3.79(s, 3H), 4.36(s, 2H), 6.84-6.88(m, 2H), 7.30-7.33(m, 2H) IR(neat)1742, 1676, 1551, 1512, 1406, 1203, 1027, 932 cm- 1 A mixed solution of 2.22 g (7.60 mmol) of acylmeldrum's acid, 30 mL of benzene, and 1.0 mL (7.65 mmol) of DL-dimethyl malate was heat-refluxed for 5 hours.

The reaction solution.was concentrated and the residue was purified by medium-pressure column chromatography (silica gel, ethyl acetate:hexane 1:3) to give 2.34 g (6.64 mmol, 87.4%) of a colorless oily P-ketoester.

1 H-NMR(300MHz, CDCl 3 2.91(d, J=6.04, 2H), 3.52(s, 2H), 93 3.72(s, 3H), 3.78(s, 3H), 3.80(m, 5H), 5.54(t, J=6.04, 1H), 6.85-6.89(m, 2H), 7.11-7.14(m, 2H) IR(neat)1750, 1516, 1439, 1251,_ 1180, 1054 cm- 1 Under similar conditions to Example 10, 2.32 g (6.58 mmol) of the p-ketoester was subjected to reaction and recrystallized from methanol to give 1.76 g of colorless crystals.

[EXAMPLE 40] Synthesis of Compound To a mixed solution of 10.0 g (69.4 mmol) of meldrum's acid, 40 mL of dichloromethane, and 11.2 mL of pyridine, was added dropwise 21.0 mL (69.5 mmol) of palmitoyl chloride while stirring the mixed solution in an ice bath. After the addition of palmitoyl chloride, the resulting mixture was stirred for 1 hour in the ice bath, removed from the ice bath, and stirred for further 24 hours. The reaction solution was mixed with 75 mL of 2 N hydrochloric acid and extracted with dichloromethane. The resultant was dried over sodium sulfate anhydride and concentrated under reduced pressure to give 25.7 g (67.3 mmol, 97.0%) of acylmeldrum's acid as colorless crystals.

A mixed solution of 6.59 g (17.2 mmol) of acylmeldrum's acid, 40 mL of benzene, and 2.74 g (17.1 mmol) of ethyl 3-acetoxy-2-hydroxypropanate was heatrefluxed for 4 hours. The reaction solution was concentrated and the residue was purified by silica gel 94 column chromatography (ethyl acetate:hexane 1:5) to give 5.04 g (11.0 mmol, 64.5%) of a P-ketoester as a pale yellow solid.

1 H-NMR(300OMHz, CDCl 3 0.88(t, J=6.31, 3H), 1.25-1.32(m, 27H), 1.60(m, 2H), 2.08(s, 3H), .2.58(t, J=7.14, 2H), 3.56(s, 2H), 4.24(m, 2H), 4.47(m, 2H), 5.36(m, 1H) IR(neat)2920, 2852, 1744, 1234 cm- 1 Under similar conditions to Example 10, 5.04 g (11.0 mmol) of the P-ketoester was subjected to reaction and recrystallized from methanol to give 3.45 g (8.39 mmol, 76.3%) of colorless crystals.

[EXAMPLE 41] Synthesis of Compound 41 A mixed solution of 2.91 g (7.61 mmol) of palmitoylmeldrum's acid, 40 mL of benzene, and 1.0 mL (7.65 mmcl) of DL-dimethyl malate was heat-ref luxed for hours. The reaction solution was concentrated and the residue was purified by medium-pressure column chromatography (silica gel, ethyl acetate:hexane 1:5) to give 2.72 g (6.14 mmol, 80.7%) of a P-ketoester as a pale yellow solid.

1 H-NMR(300MHz, CDCl 3 0.88(t, JJ=6.87, 3H), 1.25-1.32(m, 27H), 1.57(m, 2H), 2.56(t, J=7.141 3H), 2.92(d, J=5.77, 2H), 3.5(s, 2H), 3.73(s, 3H), 3.78(s, 3H), 5.55(t, J=5.77, 1H) IR(neat)2920, 2854, 1748, 1255, 1234, 1205 cm-1 95 Under similar conditions to Example 10, 2.70 g (6.10 mmol) of the P-ketoester was subjected to reaction and recrystallized from methanol to give 2.13 g of pale orange crystals.

[EXAMPLE 42] Synthesis of Compound 42 Triethylamine (0.75 mL, 5.5 mmol) was added to a suspension of tetronic acid (500 mg, 5.00 mmol) in dichloromethane (25 mL) to obtain a homogeneous solution.

The solution was cooled to OC, mixed with 4-N,Ndimethylaminopyridine (200 mg, 1.7 mmol), 3-cyclohexne-lcarboxylic acid (0.65 mL, 5.6 mmol), and then, diisopropylcarbodiimide (1.0 mL, 6.3 mmol), followed by stirring for 30 min. The resulting mixture was removed from the ice bath, stirred for 14 hours at room temperature, and filtered to remove thus-produced insoluble diisopropyl urea; the reaction solution was washed twice with 5% hydrochloric acid (20 mL) and the water layer was extracted twice with dichloromethane mL). The organic layers were combined, dried over sodium sulfate anhydride, and concentrated; the thus-obtained residue was purified by silica gel column chromatography (silica gel, ethyl acetate:methanol 15:1). The resultant was arranged to be a sodium salt by adding an excess amount of sodium hydrogencarbonate and distilled water (100 mL), and then washed with dichloromethane; the 96 water layer was acidified by adding 5% hydrochloric acid and extracted four times with dichloromethane (20 mL).

The resultant was dried over sodium sulfate anhydride and concentrated to give pale yellow crystals (394 mg, 38%).

[EXAMPLE 43] Synthesis of Compound 43 Similarly to Example 42, brown crystals (1.13 g) were obtained from tetronic acid (500 mg, 5.00 mmol) and monomethyl glutarate (0.70 mL, 5.6 mmol).

[EXAMPLE 44] Synthesis of Compound 44 Similarly to Example 42, brown crystals (744 mg, 66%) were obtained from tetronic acid (500 mg, 5.00 mmol) and 2-thiopheneacetic acid (782 mg, 5.5 mmol).

[EXAMPLE 45] Synthesis of Compound Similarly to Example 42, orange crystals (900 mg, were obtained from tetronic acid (500 mg, 5.00 mmol) and 3-thiopheneacetic acid (782 mg, 5.5 mmol).

[EXAMPLE 46] Synthesis of Compound 46 For removing mineral oil, 509 mg (12.7 mmol) of sodium hydride was washed several times with hexane and dried under reduced pressure. The resultant was mixed with 15 mL of toluene, and then, 1.15 mL (6.70 mmol) of ethyl benzoylacetate was added dropwise to the mixture while stirring the mixture in an ice bath. Stirring was carried out for 1 hour in the ice bath after the addition of ethyl benzoylacetate. To the resulting slurry, was -97 added dropwise 1 g (6.64 mmol)/3 mL of a solution of 2acetoxy-propionyl chloride in toluene. After the addition of 2-acetoxy-propionyl chloride, the mixture was stirred for 1 hour in the ice bath and further 4 hours at room temperature. The reaction was stopped by adding methanol to the reaction solution and the reaction solution was concentrated under reduced pressure. The residue was dissolved in ethyl acetate, washed with 2 N hydrochloric acid, dried over sodium sulfate anhydride, and concentrated. The residue was roughly purified by silica gel column chromatography. The thus-obtained oily substance was mixed with 12.5 mL of 2 N sodium hydroxide and stirred overnight. The reaction solution was acidified by adding 6 N hydrochloric acid, extracted with dichloromethane, dried over sodium sulfate anhydride, and concentrated. The residue was distilled by Kugel Rohr under reduced pressure (0.2 mmHg, 145°C) to give 197 mg (0.903 mmol, 13.6%) of the target compound as an orange oily substance.

[EXAMPLE 47] Synthesis of Compound 47 To a mixed solution of 4.5 mL (26.7 mmol) of diethyl malate, 20 mL of dichloromethane, and 2.4 mL of pyridine, was added dropwise 3.45 mL (26.9 mmol) of monoethyl malonyl chloride while stirring the mixed solution in an ice bath; the resulting mixture was stirred for 1 hour in 98the ice bath, removed from the ice bath, and stirred for further 3 hours. The resultant was mixed with 6 N hydrochloric acid, extracted with dichloromethane, dried over sodium sulfate anhydride, filtered, and concentrated to give 7.25 g of a malonic acid derivative. The malonic acid derivative was mixed with 30 mL of a 1 mol solution of tetrabutylammonium fluoride/tetrahydrofuran and stirred overnight at room temperature. The reaction solution was concentrated; the residue was mixed with 30 mL of 6 N hydrochloric acid and extracted with ether. After removing ether by evaporation, the residue was dissolved in dichloromethane and washed with saturated sodium chloride water. The organic layer was dried over sodium sulfate anhydride, filtered, and concentrated; the residue was purified by silica gel column chromatography (ethyl acetate:hexane As a result, 1.09 g of the target compound was obtained as an orange oily substance.

[EXAMPLE 48] Synthesis of Compound 26 Dichloromethane (40 mL) was added to a mixture of meldrum's acid (5.00 g, 34.7 mmol) and 2-thiopheneacetic acid (4.95 g, 34.7 mmol), the resulting mixture was mixed with dicyclohexylcarbodiimide (7.5 g, 36 mmol) and 4-N,Ndimethylaminopyridine (0.42 g, 3.5 mmol), and stirred for 19 hours at 25°C. After removing the thus-produced insoluble dicyclohexyl urea by filtration, the reaction 99 solution was washed with 1 N hydrochloric acid (50 mL), the water layer was extracted three times with dichloromethane (30 mL), and the organic layers were combined and dried over sodium sulfate anhydride. After concentrating the resulting solution, the thus-obtained residue was purified by silica gel column chromatography (dichloromethane) to give a substantially pure oily substance (5.04 g).

The oily substance (5.04 g) was dissolved in benzene mL), mixed with DL-diethylmalate (2.58 mL, 19.7 mmol), and heat-refluxed for 9 hours. After removing the solvent by evaporation, the residue was purified by silica gel column chromatography (hexane:ethyl acetate 4:1 to 2:1) to give an oily substance (3.53 g).

The oily substance (3.53 g) was dissolved in tetrahydrofuran (5 mL), mixed with tetrabutylammonium fluoride (1 M tetrahydrofuran, 13.0 mL, 13.0 mmol), stirred for 24 hours at room temperature. After removing the solvent by evaporation, the residue was mixed with 6 N hydrochloric acid; the thus-produced precipitate was filtered, washed with hexane, and recrystallized from ethanol to give pale brown crystals (2.41 g, 23% from meldrum's acid).

[EXAMPLE 49] Synthesis of Compound 49 At room temperature, 4-N,N-dimethylaminopyridine (74 100mg, 0.61 mmol), propionic acid (0.41 mL, 5.5 mmol), and then, dicyclohexylcarbodiimide (1.2 g, 5.8 mmol) were added to a suspension of 4-hydroxy-6-methyl-2-pyrone (634 mg, 5.02 mmol) in toluene (15 mL). After stirring for min., the resulting mixture was heated at 70°C for 18 hours. After cooling the mixture to room temperature, the thus-produced insoluble dicyclohexyl urea was removed by filtration; the reaction solution was washed with hydrochloric acid (20 mL), the water layer was extracted twice with dichloromethane (10 mL), and the organic layers were combined and dried over sodium sulfate anhydride.

The solution was concentrated and the residue was purified by silica gel column chromatography (hexane/ethyl acetate= 6/1) to give a white solid (574 mg, The solid was recrystallized from hexane/ethyl acetate to give white crystals.

[EXAMPLE 50] Synthesis of Compound Similarly to Example 49, a white solid (890 mg, 86%) was obtained from 4-hydroxy-6-methyl-2-pyrone (631 mg, 5.00 mmol) and 4-pentenoic acid (0.51 mL, 5.0 mmol). The solid was recrystallized from hexane/ethyl acetate to give white crystals.

[EXAMPLE 51] Synthesis of Compound 51 Similarly to Example 49, an orange solid (550 mg, 44%) was obtained from 4-hydroxy-6-methyl-2-pyrone (634 101 mg, 5.02 mmol) and 2-thiopheneacetic acid (0.79 mg, 5.6 mmol). The solid was recrystallized from hexane/ethyl acetatd to give pale yellow crystals.

[EXAMPLE 52] Synthesis of Compound 52 Similarly to Example 49, yellow crystals (2.88 g, were obtained from 4-hydroxy-6-methyl-2-pyrone (2.00 g, 15.9 mmol) and 2-acetoxyacetic acid (1.9 g, approximately 16 mmol, unpurified synthesized material).

[EXAMPLE 53] Synthesis of Compound 53 Similarly to Example 49, 1.27 g of colorless crystals (5.98 mg, 33.8%) were obtained from 1.99 g (17.7 mmol) of 1,3-cyclohexanedion and 2.3 mL of monomethyl glutarate (18.4 mmol).

[EXAMPLE 54] Synthesis of Compound 54 Similarly to Example 49, 1.47 g (5.27 mmol, 33.3%) of pale yellow crystals were obtained from 2.00 g (15.8 mmol) of 4-hydroxy 6-methyl-2-pyrone and 2.70 g (15.8 mmol) of 4-chlorophenylacetic acid by recrystallization from ethyl acetate.

[EXAMPLE 55] Synthesis of Compound Similarly to Example 49, a pale yellow solid (2.46 g, 91%) was obtained from 4-hydroxy-6-methyl-2-pyrone (1.50 g, 11.9 mmol) and methylthiopropionic acid (1.30 mL, 12.5 mmol). The solid was recrystallized from ethanol to give yellow crystals.

102 [EXAMPLE 56] Synthesis of Compound 56 Similarly to Example 49, a yellow solid (2.00 g, 71%) was obtained from 4-hydroxy-6-methyl-2-pyrone (1.50 g, 11.9 mmol) and dichloroacetic acid (1.25 mL, 12.1 mmol).

The solid was recrystallized from ethanol to give white crystals.

[EXAMPLE 57] Synthesis of Compound 57 Similarly to Example 49, white crystals (2.10 g, 79%) were obtained from 4-hydroxy-6-methyl-2-pyrone (1.50 g, 11.9 mmol) and tetrahydrofuran carboxylic acid (1.20 mL, 12.5 mmol).

[EXAMPLE 58] Synthesis of Compound 58 Similarly to Example 49, white crystals (1.50 g, were obtained from 4-hydroxy-6-methyl-2-pyrone (1.50 g, 11.9 mmol) and cyclohexylacetic acid (1.70 g, 12.0 mmol).

[EXAMPLE 59] Synthesis of Compound 59 Similarly to Example 49, white crystals (1.30 g, 46%) were obtained from 4-hydroxy-6-methyl-2-pyrone (1.50 g, 11.9 mmol) and cyclopentylacetic acid (1.50 mL, 11.9 mmol).

[EXAMPLE 60] Synthesis of Compound Similarly to Example 49, yellow crystals (0.75 g, 26%) were obtained from 4-hydroxy-6-methyl-2-pyrone (1.50 g, 11.9 mmol) and phenylacetyl chloride (1.57 mL, 11.9 mmol).

103 [EXAMPLE 61] Synthesis of Compound 61 Similarly to Example 49, yellow crystals (300 mg, were obtained from 4-hydroxy-6-methyl-2-pyrone (2.00 g, 15.9 mmol) and butyric acid (1.45 mL, 15.9 mmol).

[EXAMPLE 62] Synthesis of Compound 62 Similarly to Example 49, yellow crystals (2.44 g, 73%) were obtained from 4-hydroxy-6-methyl-2-pyrone (2.00 g, 15.9 mmol) and n-valerianic acid (1.72 mL, 15.8 mmol).

[EXAMPLE 63] Synthesis of Compound 63 Similarly to Example 49, white crystals (450 mg, 13%) were obtained from 4-hydroxy-6-methyl-2-pyrone (2.00 g, 15.9 mmol) and monomethyl malonate (1.87 g, 15.9 mmol).

[EXAMPLE 64] Synthesis of Compound 64 Similarly to Example 49, white crystals (600 mg, 19%) were obtained from 4-hydroxy-6-methyl-2-pyrone (2.00 g, 15.9 mmol) and isobutyric acid (1.40 mL, 15.1 mmol).

[EXAMPLE 65] Synthesis of Compound Similarly to Example 49, white crystals (1.04 g, 69%) were obtained from 4-hydroxy-6-methyl-2-pyrone (657 mg, 5.21 mmol) and 2-(4-methylthiophenyl)acetic acid (950 mg, 5.21 mmol).

[EXAMPLE 66] Synthesis of Compound 66 Similarly to Example 49, yellow crystals (2.16 g, 43%) were obtained from 4-hydroxy-6-methyl-2-pyrone (2.00 g, 15.9 mmol) and N-phthaloyl glycine (3.25 g, 15.8 mmol).

LU i; 104 [EXAMPLE 67] Synthesis of Compound 67 From 2.00 g (15.8 mmol) of 5-methyl-1, 3 cyclohexanedione and 1.2 mL (16.2 mmol) of propionic acid, 1.71 g (9.38 mmol, 59.3%) of 5-methyl-2-propionyl-1, 3 cyclohexanedione was obtained as colorless crystals similarly to Example 49.

[EXAMPLE 68] Synthesis of Compound 68 From 2.00 g (15.8 mmol) of 5-methyl-1,3cyclohexanedione and 0.95 mL (16.2 mmol) of acetic acid, 1.29 g (7.67 mmol, 48.5%) of 2-acetyl-5-methyl-l, 3 cyclohexanedione was obtained as colorless crystals similarly to Example 49.

[EXAMPLE 69] Synthesis of Compound 69 From 2.00 g (15.8 mmol) of 5-methyl-l,3cyclohexanedione and 2.25 g (15.9 mmol) of thiopheneacetic acid, 1.22 g (4.87 mmol, 30.8%) of 5-methyl-2-propionyl- 1,3-cyclohexanedione was obtained as pale yellow crystals similarly to Example 49.

[EXAMPLE 70] Synthesis of Compound Similarly to Example 49, yellow crystals (3.58 g, were obtained from 4-hydroxy-6-methyl-2-pyrone (1.80 g, 14.3 mmol) and 5-(2-thiophene)-butyric acid (2.1 mL, 14.3 mmol).

[EXAMPLE 71] Synthesis of Compound 71 In an argon atmosphere, sodium hydride (5.2 g, 105 was washed with tetrahydrofuran, and to the resulting suspension in tetrahydrofuran (200 mL), was added dropwise a solution of ethyl acetoacetate (12.6 mL, 100 mmol) in tetrahydrofuran (10 mL) at room temperature. After the addition of the ethyl acetoacetate solution, stirring was carried out for 1 hour at room temperature. The solution' was cooled to 0 C and butyl lithium (2.5 M hexane solution, 42 mL, 105 mmol) was added dropwise to the solution; after the addition of butyl lithium, the mixture was stirred for 20 min. as it was, and then, for further min. at room temperature. The solution was cooled to 0 C again, mixed with acetone (7.4 mL, 100 mmol), and stirred for 20 min. at room temperature. By adding hydrochloric acid and then concentrated hydrochloric acid, the resulting mixture was acidified, and extracted with ethyl acetate; the organic layer was dried over sodium sulfate anhydride. The solution was concentrated and the thus-obtained oily substance was mixed with a 1 N sodium hydroxide solution (200 mL) and methanol, followed by stirring for 4 hours at room temperature. The reaction solution was concentrated as it was, evaporated to remove methanol, acidified by adding 5% hydrochloric acid, and extracted with dichloromethane; the organic layer was dried over sodium sulfate anhydride. The resulting solution was concentrated and the thus-obtained solid was 106 recrystallized from hexane-ethyl acetate to give white crystals (4.8 g, 34%).

[EXAMPLE 72] Synthesis of Compound 72 From 4-hydroxy-6-methyl-2-pyrone (2.00 g, 15.9 mmol) and N-Boc-glycine (2.78 g, 15.9 mmol), white crystals (1.25 g, 28%) were obtained similarly to Example 49.

[EXAMPLE 73] Synthesis of Compound 73 From 4-hydroxycoumarin (2.00 g, 12.3 mmol) and acetic acid (0.71 mL, 12.3 mmol), white crystals (1.12 g, 49%) were obtained similarly to Example 49.

[EXAMPLE 74] Synthesis of Compound 74 From Compound 71 (1.00 g, 7.03 mmol) and 2thiopheneacetic acid (1.00 g, 7.03 mmol), white crystals (1.27 g, 68%) were obtained similarly to Example 49.

[EXAMPLE 75] Synthesis of Compound Similarly to Example 49, 1.66 g (8.37 mmol, 53.0%) of 2-methoxyacetyl-5-methyl-l,3-cyclohexanedion was obtained as a pale yellow liquid from 2.00 g (15.8 mmol) of methyl-l,3-cyclohexanedion and 1.22 mL (15.9 mmol) of methoxyacetic acid.

[EXAMPLE 76] Synthesis of Compound 76 In 500 mL of dichloromethane, was dissolved 22.0 mL (207 mmol) of 2-pentanone. While stirring the resulting solution in an ice bath, 44 mL (317 mmol) of triethylamine was added to the solution, and then, 42 mL (232 mmol) of 107 trimethylsilyl trifluoromethanesulfonate was added dropwise to the solution. After the addition of trimethylsilyl trifluoromethanesulfonate, the mixture was stirred for 1 hour at room temperature, poured into ether (500 mL)/a saturated aqueous sodium hydrogencarbonate solution (300 mL) so as to extract the ether layer. The ether layer was washed with saturated sodium chloride water and water, dried over sodium sulfate anhydride, filtered, and concentrated. The residue was distilled under normal pressure to collect fractions of 110 to 125*C. As a result, 8.43 g (53.2 mmol, 49.4%) of 2trimethylsilyloxy-2-pentene was obtained. According to a similar method, 5.33 g (33.6 mmol, 32.3%) of 2trimethylsilyloxy-2-pentene was obtained from 11.0 mL (103.8 mmol) of 2-pentanone.

In 200 mL of ether, was dissolved 13.6 g (85.9 mmol) of 2-trimethylsilyloxy-2-pentene, and 4.6 mL (47.3 mmol) of malonyl chloride was added dropwise to the solution while stirring the solution at -78 0 C. After the addition of malonyl chloride, the solution was stirred for 1 hour at -78*C, and then, stirred over night while gradually heating the solution to room temperature. The precipitate was filtered and washed with ether. As a result, 3.08 g (20.0 mmol, 42.3%) of 5-ethyl-4-hydroxy-6-methyl-2-pyrone was obtained.

S 108- [EXAMPLE 77] Synthesis of Compound 77 Similarly to Example 49, 1.03 g (5.25 mmol, 81.0%) of 3-acetyl-5-ethyl-4-hydroxy-6-methyl-2-pyrone was obtained as a pale yellow liquid from Compound 76 (1.00 g, 6.48 mmol) and 0.38 mL (6.55 mmol) of acetic acid.

[EXAMPLE 78] Synthesis of Compound 78 Similarly to Example 49, 1.11 g (5.28 mmol, 81.5%) of 5-ethyl-4-hydroxy-6-methyl-3-propanoyl-2-pyrone was obtained as a pale yellow oily substance from Compound 76 (1.00 g, 6.48 mmol) and 0.5 mL (6.48 mmol) of propionic acid.

[EXAMPLE 79] Synthesis of Compound 79 Similarly to Example 49, white crystals (0.96 g, 76%) were obtained from Compound 71 (1.00 g, 7.03 mmol) and acetic acid (0.40 mL, 7.03 mmol).

[EXAMPLE 80] Synthesis of Compound Similarly to Example 49, yellow crystals (1.21 g, 24%) were obtained from 4-hydroxy-6-methyl-2-pyrone (2.00 g, 15.9 mmol) and p-toluenesulfonylacetic acid (3.40 g, 15.9 mmol).

[EXAMPLE 81] Synthesis of Compound 81 Similarly to Example 49, white crystals (0.71 g, were obtained from 4-hydroxy-6-methyl-2-pyrone (2.00 g, 15.9 mmol) and phthalide-3-acetic acid (3.04 g, 15.9 109 mmol).

[EXAMPLE 82] Synthesis of Compound 82 Similarly to Example 49, yellow crystals (0.96 g, 26%) were obtained from 4-hydroxy-6-methyl-2-pyrone (2.00 g, 15.9 mmol) and 4-cyclohexenecarboxylic acid (1.9 mL, 15.9 mmol).

[EXAMPLE 83] Synthesis of Compound 83 Similarly to Example 49, 608 mg (2.17 mmol, 33.5%) of 5-ethyl-4-hydroxy-6-methyl-3-(2-thiopheneacetyl)-2-pyrone was obtained as an orange oily substance from Compound 76 (1.00 g, 6.48 mmol) and 922 mg (6.48 mmol) of 2thiopheneacetic acid.

[EXAMPLE 84] Synthesis of Compound 84 When 7.0 mL (72.0 mmol) of malonyl chloride was added to 7.0 mL (67.8 mmol) of acetylacetone, a violent exothermic reaction occurred to give a black solid within a few minutes. The solid was cooled to room temperature, washed with ether and then ethyl acetate, and filtered.

The thus-obtained brown solid was recrystallized from ethyl acetate/methanol to give 1.75 g (10.4 mmol, 15.3%) of 5-acetyl-4-hydroxy-6-methyl-2-pyrone as brown crystals.

The filtrates from washing and the recrystallized mother liquor were combined and concentrated; the residue was purified by silica gel column chromatography to give 2.87 g .(17.1 mmol, 25.2%) of 5-acetyl- 4 -hydroxy-6-methyl-2- 110 pyrone as orange crystals.

[EXAMPLE 85] Synthesis of Compound Similarly to Example 49, 242 mg (1.15 mmol, 19.3%) of 3,5-diacetyl-4-hydroxy-6-methyl-2-pyrone was obtained as pale yellow needle crystals from Compound 84 (1.00 g, 5.95 mmol) and 0.35 mL (6.11 mmol) of acetic acid by recrystallization from hexane.

[COMPARATIVE EXAMPLE 1] Synthesis .of 1-oxa-2,4- In an argon atmosphere, sodium hydride (5.2 g) was washed with tetrahydrofuran, and to the resulting suspension in tetrahydrofuran (200 mL), was added dropwise a solution of ethyl acetoacetate (12.6 mL, 100 mmol) in tetrahydrofuran (10 mL) at room temperature. After the addition of the ethyl acetoacetate solution, stirring was carried out for 1 hour at room temperature. The solution was cooled to 0°C and butyl lithium (2.5 M hexane solution, 44 mL, 110 mmol) was added dropwise to the solution; after the addition of butyl lithium, the solution was stirred for 20 min., and then, for further min. at room temperature. The solution was cooled to 0 C again, mixed with cyclohexanone (11.5 mL, 110 mmol), and stirred at room temperature for 30 min. By adding hydrochloric acid and then concentrated hydrochloric acid, the resulting mixture was acidified, mixed with sodium 111 chloride, and extracted with ethyl acetate; the organic layer was dried over sodium sulfate anhydride. The solution was concentrated and the thus-obtained oily substance was mixed with a 1 N aqueous sodium hydroxide solution (200 mL) and methanol, followed by stirring for 4 hours at room temperature. The reaction solution was concentrated as it was and evaporated to remove methanol, acidified by adding 5% hydrochloric acid, and extracted with dichloromethane; the organic layer was dried over sodium sulfate anhydride. The solution was concentrated and the thus-obtained solid was recrystallized from hexane-ethyl acetate to give white crystals (9.96 g, [EXAMPLE 86] Synthesis of Compound 86 Similarly to Example 49, white crystals (697 mg, 71%) were obtained from l-oxa-2,4-oxospiro[5,5]undecane (800 mg, 4.39 mmol) and acetic acid (0.25 mL, 4.39 mmol).

[EXAMPLE 87] Synthesis of Compound 87 Similarly to Example 49, white crystals (0.83 g, 53%) were obtained from 6-phenetyl-4-oxa-6-valerolactone (1.00 g, 4.58 mmol) and 2-thiopheneacetic acid (650 mg, 4.58 mmol).

[EXAMPLE 88] Synthesis of Compound 88 Similarly to Example 49, white crystals (3.39 g, 91%) were obtained from 4-hydroxy-6-methyl-2-pyrone (2.00 g, 15.9 mmol) and thenoyl chloride (1.96 mL, 15.9 mmol) by 112 recrystallization from ethanol.

[EXAMPLE 89] Synthesis of Compound 89 In 150 mL of acetic acid, were suspended 11.7 g (121 mmol) of furfural and 16.25 g (122 mmol) of rhodanine. To the suspension, was added 31.0 g (378 mmol) of sodium acetate, and the mixture was stirred at 85°C for 1 hour and 40 min. After confirming the appearance of a bright orange precipitate, the reaction suspension was cooled to room temperature and poured into 200 mL of water. After thorough stirring, the resulting precipitate was filtered under reduced pressure, and the residue was washed with 300 mL of water, 20 mL of 99.5% ethanol, and then 10 mL of diethyl ether. The residue was dried to give 20.0 g (94.8 mmol) of furfural rhodanine with a crude yield of 78%.

The furfural rhodanine was used for the subsequent reaction without being further purified.

After 20.0 g (94.8 mmol) of furfural rhodanine was suspended in 120 mL of a 4.8 N aqueous sodium hydroxide solution, the suspension was stirred at 80 0 C for 1 hour and 30 min. While the reaction solution was stirred in an ice bath, 4 N hydrochloric acid.(200 mL) was poured into the reaction solution at once; the resultant was stirred at room temperature and extracted with ether. The ether layer was washed with water, a 10% aqueous sodium thiosulfate solution, and saturated sodium chloride water 113 in that order, dried over sodium sulfate anhydride, and concentrated under reduced pressure. The thus-obtained solid was recrystallized from ethanol to give 12.0 g (70.6 mmol) of 3-a-furyl-2-thioketopropionic acid with a yield of 1H-NMR(300MHz, CDC1 3 4.73(1H, 6.58(1H, 6.88(1H, 7.61(1H, 7.72(1H, s) To 200 mL of ethanol, were slowly added 8.0 g (350 mmol) of finely chopped sodium chips over 3 hours, and after sodium ethoxide was produced, the mixture was stirred for further 1 hour and 30 min. To the resulting solution, was added 23.2 g (333 mmol) of hydroxylamine hydrochloride at room temperature, and then, stirred for 1 hour. To the resulting suspension, was added dropwise 120 mL of a solution of 10.7 g (63.1 mmol) of 3-a-furyl-2thioketopropionic acid in ethanol, and then stirred for 3 hours while being heat-refluxed. The reaction suspension was cooled to room temperature and poured into 60 mL of a aqueous sodium hydroxide solution. After the precipitate was removed by filtration under reduced pressure, the filtrate was acidified by adding 80 mL of 4 N hydrochloric acid and extracted with ether. The ether layer was dried over magnesium sulfate anhydride and filtered under reduced pressure to give 7.62 g (38.7 mmol) of ethyl 3-a-furyl-2-oxyminopropionate.

114 'H-NMR(300MHz, CDC1 3 1.213(3H, t, J=7.2Hz), 3.49(2H, q, J=7.2Hz), 4.093(1H, 6.15(1H, 6.29(1H, 7.31(1H, m) To a flask, was added 7.62 g (38.7 mmol) of the thusobtained ethyl 3-a-furyl-2-oxyminopropionate and it was mixed with 85 mL of acetic acid anhydride and 1 mL of water, followed by thorough stirring. The mixture was heat-refluxed for 7 hours. After the completion of the reaction, the mixture was cooled to room temperature and subjected to steam distillation. While heating a still pot at 125"C and stirring the mixture, fractions each 350 mL were collected. Each fraction was extracted with ether (150 mL x thus-collected ether was concentrated under reduced pressure to approximately a third, neutralized by adding a saturated aqueous sodium hydrogencarbonate solution, and dried over magnesium sulfate anhydride. The target compound was contained in the first to the fourth fractions collected from steam distillation. Then, 4.4 g of the thus-obtained residue was subjected to silica gel column chromatography, using 100 g of silica gel and hexane/dichloromethane 1/1 to 1/2 as a solvent, to give 3.79 g (35.4 mmol) of 2-a-furylacetonitrile with a yield of 91.5.

1H-NMR(300MHz, CDCl 3 3.77(2H, 6.33(1H, 6.37(1H, 7.40(1H, m) 115- IR(KBr)2260, 1603cm- 1 In a mixed solution of 30 mL of a 3 N sodium hydroxide solution and 30 mL of methanol, was dissolved 3.34 g (31.2 mmol) of 2-a-furylacetonitrile and the resulting solution was heat-refluxed for 2 hours and min. while stirring the solution. The solution was cooled to room temperature and extracted with ether. The water layer was mixed with 20 mL of concentrated hydrochloric acid to liberate organic substances, followed by extraction with ether. The ether layer was collected, dried over magnesium sulfate anhydride, concentrated under reduced pressure to give 3.84 g (30.5 mmol) of 2-afurylacetic acid with a yield of 97.8%.

In 100 mL of toluene, were dissolved 1.00 g (7.91 mmol) of the thus-prepared 2-a-furylacetic acid and 1.04 g (8.24 mmol) of 4-hydroxy-6-methyl-2-pyrone, and then, 1.65 g (7.99 mmol) of dicyclohexylcarbodiimide and 0.18 g (1.44 mmol) of 4-N dimethylaminopyridine were added to the resulting solution, followed by stirring at room temperature for 1 hour and at 80 0 C for 14 hours. The resultant was cooled to room temperature, filtered to remove residue, and the thus-obtained filtrate was concentrated under reduced pressure, followed by extraction with dichloromethane. The resultant was washed with a saturated aqueous ammonium chloride solution and a 116 saturated aqueous sodium chloride solution, dried over sodium sulfate anhydride, and concentrated under reduced pressure to give 2.35 g of a mixed product. The product was purified by silica gel column chromatography using 108 g of silica gel. Hexane/ethyl acetate 3/1 to 1/1 was used as a solvent; the thus-obtained substance was concentrated and dissolved in ethyl acetate. With a saturated aqueous sodium hydrogencarbonate solution, the target compound was extracted to the water layer, and then, the water layer was acidified by adding hydrochloric acid, followed by extraction with ethyl acetate. By recrystallization from ethanol, 0.52 g of the target compound was obtained.

[EXAMPLE 90] Synthesis of Compound Similarly to Example 49, pale yellow crystals (1.10 g, 32%) were obtained from 4-hydroxy-6-methyl-2-pyrone (2.00 g, 15.9 mmol) and 2-methylthioacetic acid (1.3 mL, 16 mmol).

[EXAMPLE 91] Synthesis of Compound 91 Similarly to Example 49, yellow crystals (950 mg, 23%) were obtained from 4-hydroxy-6-methyl-2-pyrone (2.00 g, 15.9 mmol) and 2-phenylpropionic acid (2.17 g, 15.9 mmol).

[EXAMPLE 92] Synthesis of Compound 92 Similarly to Example 49, white crystals (4.66 g, 67%) 117 were obtained from 4-hydroxy-6-methyl-2-pyrone (3.00 g, 23.8 mmol) and N-undecanoic acid (4.8 g, 24 mmol).

[EXAMPLE 93] Synthesis of Compound 93 Similarly to Example 49, white crystals (3.43 g, 64%) were obtained from 4-hydroxy-6-methyl-2-pyrone (3.00 g, 23.8 mmol) and tetrahydro-3-furancarboxylic acid (2.25 mL, 23.8 mmol).

[EXAMPLE 94] Synthesis of Compound 94 Similarly to Example 49, white crystals (5.05 g, 58%) were obtained from 4-hydroxy-6-methyl-2-pyrone (3.00 g, 23.8 mmol) and palmitic acid (6.10 g, 23.8 mmol).

[EXAMPLE 95] Synthesis of Compound Similarly to Example 49, white crystals (3.13 g, 81%) were obtained from 4-hydroxycoumarin (2.00 g, 12.3 mmol) and capric acid (2.13 g, 12.3 mmol).

[EXAMPLE 96] Synthesis of Compound 96 Similarly to Example 49, white crystals (3.13 g, were obtained from 4-hydroxycoumarin (2.00 g, 12.3 mmol) and palmitic acid (3.16 g, 12.3 mmol).

[EXAMPLE 97] Synthesis of Compound 97 Similarly to Example 49, pale yellow crystals (430 mg, 16%) were obtained from 4-hydroxy-6-methyl-2-pyrone (1.00 g, 7.93 mmol) and 3-(6-chlorobenzothiophene)acetic acid (1.80 g, 7.93 mmol).

[EXAMPLE 98] Synthesis of Compound 98 118 Similarly to Example 49, pale yellow crystals (1.48 g, 33%) were obtained from 4-hydroxy-6-methyl-2-pyrone (2.00 g, 15.9 mmol) and 3-indoleacetic acid (2.8 g, 16 mmol).

[EXAMPLE 99] Synthesis of Compound 99 Similarly to Example 49, pale yellow crystals (6.21 g, 57%) were obtained from 4-hydroxy-6-methyl-2-pyrone (5.00 g, 39.7 mmol) and phenylthioacetic acid (6.7 g, mmol).

[EXAMPLE 100] Synthesis of Compound 100 Similarly to Example 49, pale yellow-crystals (1.63 g, 44%) were obtained from 4-hydroxy-6-methyl-2-pyrone (1.50 g, 11.9 mmol) and 3-methylbenzo[b]thiophene-2-acetic acid (2.45 g, 11.9 mmol).

[EXAMPLE 101] Synthesis of Compound 101 Similarly to Example 49, 1.82 g of the target compound was obtained from 2.20 g (11.8 mmol) of undecanoic acid and 1.20 g (12.0 mmol) of tetronic acid by recrystallization from methanol.

[EXAMPLE 102] Synthesis of Compound 102 Similarly to Example 49, 0.58 g of crystals were obtained from 1.22 g (7.71 mmol) of pelargonic acid and 0.78 g (7.79 mmol) of tetronic acid by recrystallization from methanol.

[EXAMPLE 103] Synthesis of Compound 103 119 Similarly to Example 49, 0.62 g of the target compound was obtained from capric acid (0.97 g, 6.7 mmol) and tetronic acid (0.71 g, 7.1 mmol) by recrystallization from methanol.

[EXAMPLE 104] Synthesis of Compound 104 Similarly to Example 49, pale yellow crystals (5.03 g, 44%) were obtained from 4-hydroxy-6-methyl-2-pyrone (5.00 g, 40.0 mmol) and 4-nitrophenylacetic acid (7.2 g, mmol).

[EXAMPLE 105] Synthesis of Compound 105 Similarly to Example 49, pale yellow crystals (2.50 g, 57%) were obtained from 4-hydroxy-6-methyl-2-pyrone (2.00 g, 15.9 mmol) and 4-methoxyphenylacetic acid (2.65 g, 15.9 mmol).

[EXAMPLE 106] Synthesis of Compound 106 Similarly to Example 49, pale yellow crystals (2.57 g, 29%) were obtained from 4-hydroxy-6-methyl-2-pyrone (5.00 g, 40.0 mmol) and levulinic acid (4.1 mL, 40 mmol).

[EXAMPLE 107] Synthesis of Compound 107 Similarly to Example 49, pale yellow crystals (3.22 g, 52%) were obtained from 4-hydroxy-6-methyl-2-pyrone (3.00 g, 23.8 mmol) and 4-fluorophenylacetic acid (3.7 g, 24 mmol).

[EXAMPLE 108] Synthesis of Compound 108 In an argon atmosphere, 1.06 g (9.45 mmol) of 120hexynoic acid was dissolved in 90 mL of dry tetrahydrofuran and the resulting solution was cooled to 78 0 C. To the solution, was added dropwise 10 mL (25.0 mmol) of a 5 N butyl lithium hexane solution, followed by stirring at -78°C for 15 min. After that, the solution was mixed with 10 mL of HMPA and stirred; then 20 mL of a solution of 1.5 mL (d=1.617, 2.42 g, 13.2 mmol) of butyl iodide in tetrahydrofuran was added dropwise to the solution. The reaction solution was stirred at -78*C for 1 hour, and then, stirred overnight while gradually elevating the temperature to room temperature. The reaction solution was poured into ice-i N hydrochloric acid and extracted with dichloromethane. The resultant was washed with saturated sodium bicarbonate water to liberate acidic components; the water layer was acidified by adding concentrated hydrochloric acid and extracted with dichloromethane again. The collected organic layers were washed with a 10% aqueous sodium thiosulfate solution and a saturated aqueous sodium chloride solution in that order, dried over manganese sulfate anhydride, and concentrated under reduced pressure to give a crude product. The product was used in the subsequent reaction without being further purified. The thus-obtained crude product was dissolved in 50 mL of dry methanol, mixed with 1 mL of concentrated sulfuric acid, and heat-refluxed for 121 2 hours. The resultant was cooled, poured into saturated sodium bicarbonate water, and extracted with ethyl acetate. The resultant was washed with water, a saturated aqueous ammonium chloride solution, and a saturated aqueous sodium chloride solution in that order, dried over manganese sulfate anhydride, and concentratedunder reduced pressure. The residue was purified by silica gel column chromatography using 110 g of silica gel.

Hexane/ethyl acetate was used as a solvent to give 1.02 g of the target compound.

1H-NMR(300MHz, CDC1 3 0.91(3H, t, J=6.8Hz), 1.4(m, 4H), 1.80(2H, q, J=7.4Hz), 2.14(2H, 2.22(2H, 2.44(2H, t, J=7.4Hz), 3.68(3H, s) To a 2.1% aqueous sodium hydroxide solution, was added 0.99 g (5.4 mmol) of methyl 5-decynate, and the mixture was heat-refluxed for 3 hours. The reaction solution was acidified by adding concentrated hydrochloric acid and extracted with ether. The organic layer was washed with a saturated aqueous sodium chloride solution, dried over magnesium sulfate, and concentrated under reduced pressure to give 0.87 g of the target compound.

1 H-NMR(300MHz, CDC1 3 0.91(3H, t, J=7.1Hz), 1.4(4H, m), 1.81(2H, q, J=7.4Hz), 2.14(2H, 2.24(2H, 2.50(2H, t, J=7.4Hz) 122 Similarly to Example 49, 0.51 g of the target compound was obtained from 5-decynoic acid (0.87 g, 5.1 mmol) and tetronic acid (0.52 g, 5.2 mmol).

[EXAMPLE 109] Synthesis of Compound 109 To a 100 mL, two-neck flask, was added 1.32 g of sodium hydride, and it was mixed with 10 mL of hexane in an argon atmosphere, followed by stirring using a magnetic stirrer so as to wash off paraffin contained in NaH.

Hexane used for washing was removed from the flask by a Pasteur pipet. This procedure was repeated twice, and then, 50 mL of tetrahydrofuran was added to the flask.

Using a Pasteur pipet, 4.48 g of ethyl ester of diethylphosphonoacetic acid was added slowly to the mixture. When hydrogen stopped being generated, 2.96 g of 4-isopropylbenzaldehyde was added slowly to the mixture.

(Generated heat was cooled by icy water.) After 1 hour, stirring was stopped, and the reaction solution was added to 150 mL of distilled water. Extraction was carried out three times using 50 mL of ethyl acetate. The ethyl acetate layers were washed twice with 50 mL of distilled water, dried over sodium sulfate, concentrated by an evaporator, and dried by a vacuum pump. As a result, 3.4 g of ethyl p-isopropylcinnamate was obtained.

To 100 mL of ethyl acetate, were added 3.0 g of ethyl p-isopropylcinnamate and 300 mg of 5%-Pd/C. The resulting 123 mixture was stirred in a hydrogen atmosphere. Three hours after the reaction, the hydrogen atmosphere was replaced with argon atmosphere, and the resulting mixture was filtered, concentrated, and dried. As a result, 2.9 g of ethyl p-isopropyldehydrocinnamate was obtained. To 2.9 g of ethyl p-isopropyldehydrocinnamate, were added 20 mL of distilled water and 20 mL of tetrahydrofuran, and the mixture was hydrolyzed by 0.70 g of lithium hydroxide to give 2.1 g of pisopropyldehydrocinnamic acid.

Similar to Example 49, 0.211 g of the target compound was obtained from 0.968 g (5.0 mmol) of pisopropyldehydrocinnamic acid and tetronic acid.

[EXAMPLE 110] Synthesis of Compound 110 To 200 mL of methanol, was added 6.3 g of phydroxyphenylacetic acid. Ten droplets of concentrated hydrochloric acid was added to the resulting mixture by a Pasteur pipet and reaction proceeded at 80 0 C for 1 hour.

The mixture was concentrated, mixed with 100 mL of ethyl acetate, and washed twice with 50 mL of distilled water.

The resultant was dried over sodium sulfate, filtered, concentrated, and dried under reduced pressure. As a result, 6.5 g of methyl p-hydroxyphenylacetate was obtained.

In an argon atmosphere, 1.32 g of sodium hydride was 124 weighed, mixed with 10 mL of hexane, and stirred by using a magnetic stirrer so as to wash off paraffin contained in sodium hydride. Hexane used for washing was removed from the flask by a Pasteur pipet. This procedure was repeated twice, and then, 50 mL of dimethylformamide was added to the flask. By using a Pasteur pipet, 2.49 g of methyl phydroxyphenylacetate was added slowly to the mixture.

When hydrogen stopped being generated, 2.27 g of 1-bromo- 3-methylbuthane was added slowly to the mixture. After stirring overnight, the reaction solution was added to 150 mL of distilled water. The resultant was neutralized by adding 1 N hydrochloric acid and extracted three times with 80 mL of ether. The ether layers were washed twice with 50 mL of distilled water, dried over sodium sulfate, concentrated by an evaporator, and dried by a vacuum pump.

As a result, 1.4 g of an ether compound was obtained.

To 1.4 g of the ether compound, were added 10 mL of distilled water and 5 mL of ethanol, and the resultant was hydrolyzed by adding 0.32 g of sodium hydroxide and heating at 50 0 C to give 0.533 g of a carboxylic acid.

Similarly to Example 49, 0.173 g of the target compound was obtained from 0.389 g (1.75 mmol) of the carboxylic acid and 0.176 g (1.75 mmol) of tetronic 125 acid.

[EXAMPLE 111] Synthesis of Compound 111 Similarly to Example 49, pale yellow crystals (495 mg, 11%) were obtained from 4-hydroxy-6-methyl-2-pyrone (2.00 g, 15.9 mmol) and 4-dimethylaminophenylacetic acid (2.85 g, 15.9 mmol).

[EXAMPLE 112] Synthesis of Compound 112 Similarly to Example 49, white crystals (1.60 g, were obtained from 4-hydroxy-6-methyl-2-pyrone (2.00 g, 15.9 mmol) and 4-(trifluoromethyl)phenylacetic acid (3.24 g, 15.9 mmol).

[EXAMPLE 113] Synthesis of Compound 113 Similarly to Example 49, a brown oily substance (7.08 g) was obtained from 4-hydroxy-6-methyl-2-pyrone (5.00 g, 40.0 mmol) and diethylphosphonoacetic acid (6.4 mL, mmol). However, since complete purification was impossible, the oily substance was subjected to the subsequent reaction as it was.

1 H-NMR(300MHz, CD30D)6: 2.35(d, J=0.82Hz, 3H), 2.39(m, 3H), 4.71(s, 2H), 6.22(q, J=0.82Hz, 1H), 7.29-7.42(m, 2H), 7.71-7.74(m, 1H), 7.78-7.82(m, 1H).

The oily substance (7.08 g) was dissolved in dichloromethane (20 mL) in an argon atmosphere, mixed with bromotrimethylsilane (11 mL, 85 mmol), and stirred for 24 hours at room temperature. After stopping the reaction by 126 adding absolute methanol, the mixture was stirred for 1 hour in an argon atmosphere, and evaporated under reduced pressure for removing the solvent. The thus-obtained solid was recrystallized from ethyl acetate/ethanol to give pale brown crystals (3.47 g, [EXAMPLE 114] Synthesis of Compound 114 Similarly to Example 49, pale yellow crystals (3.77 g, 36%) were obtained from 4-hydroxy-6-methyl-2-pyrone (5.00 g, 40.0 mmol) and pelargonic acid (6.9 mL, 40 mmol).

[EXAMPLE 115] Synthesis of Compound 115 To give 0.116 g of the target compound, 3.32 g mmol) of the p-hydroxyphenylacetate used in Example 110 was reacted and the succeeding steps were followed similarly to Example 110.

[EXAMPLE 116] Synthesis of Compound 116 To 5 mL of thionyl glutarate, was added 2.92 mmol) of monomethyl glutarate and then the mixture was heated at 80 0 C for 30 min. Thionyl chloride was removed by distillation. To 2.54 g (20 mmol) of diisobutylamine, was added 20 mL of pyridine, and then the mixture was stirred. The monomethyl ester of glutaryl chloride synthesized by the above procedure was slowly added to the mixture, and then stirred overnight. After removing pyridine by evaporation, the resultant was mixed with mL of distilled water and acidified by adding 1 N HC1.

127 The mixture was extracted with ethyl acetate, dried over sodium sulfate, filtered, concentrated, and dried under reduced pressure. As a result, 4.1 g of a methyl ester of 3-(N,N-diisopropylamino)butyric acid was obtained.

By using lithium hydroxide, 2.03 g (7.89 mmol) of the above methyl ester was hydrolyzed to give 1.4 g of 3-(N,N-diisopropylamino)butyric acid. Similarly to Example 109, 0.8 g (3.29 mmol) of the above acid was subjected to reaction to give 0.154 g of the target compound.

[EXAMPLE 117] Synthesis of Compound 117 Similarly to Example 49, 0.170 g of the target compound was obtained from 0.961 g (5.0 mmol) of isopropyldehydrocinnamic acid and 0.63 g (5.0 mmol) of 4hydroxy-6-methylpyrone.

[EXAMPLE 118] Synthesis of Compound 118 Similarly to Example 49, white crystals (6.5 g, 72%) were obtained from 4-hydroxycoumarin (5.00 g, 31.3 mmol) and p-tolueneacetic acid (4.7 g, 31.3 mmol).

[EXAMPLE 119] Synthesis of Compound 119 Similarly to Example 49, white crystals (7.51 g, 66%) were obtained from 4-hydroxy-6-methyl-2-pyrone (5.00 g, 39.6 mmol) and o-nitrophenylacetic acid (7.20 g, 39.6 mmol).

128 [EXAMPLE 120] Synthesis of Compound 120 Similarly to Example 49, white crystals (0.85 g, were obtained from 4-hydroxy-6-methyl-2-pyrone (1.50 g, 11.9 mmol) and 3,4-(methylenedioxy)phenylacetic acid (2.15 g, 11.9 mmol).

[EXAMPLE 121] Synthesis of Compound 121 To a mixture of meldrum's acid (8.00 g, 55.5 mmol) and p-nitrophenylacetic acid (10.3 g, 56 mmol), was added dichloromethane (120 mL); the resultant was mixed with dicyclohexylcarbodiimide (12 g, 59 mmol) and 4-N,Ndimethylaminopyridine (1.0 g, 8.2 mmol) and stirred at 0 C for 19 hours. After removing the thus-produced insoluble dicyclohexyl urea by filtration, the reaction solution was washed with 1 N hydrochloric acid (50 mL), the water layer was extracted three times with dichloromethane (30 mL), and the organic layers were combined and dried over sodium sulfate anhydride. After concentrating the solution, the thus-obtained residue was purified by silica gel column chromatography (dichloromethane) to give substantially pure crystals (3.8 g).

1 H-NMR(300MHz, CDCl 3 1.74(s, 6H), 4.51(s, 2H), 7.58(d, J=8.8Hz, 2H), 8.22(d, J=8.8Hz, 2H).

The crystals (3.8 g, 12.5 mmol) were dissolved in benzene (80 mL), mixed with DL-diethylmalate (1.72 mL, 13 129mmol), and heat-refluxed for 4.5 hours. After removing the solvent by evaporation, the residue was purified by silica gel column chromatography (silica gel, hexane/ethyl acetate 4:1 to 2:1) to give an oily substance (4.30 g).

'H-NMR(300MHz, CDC13)6: 1.74(s, 6H), 4.51(s, 2H), 7.58(d, J=8.8Hz, 2H), 8.22(d, J=8.8Hz, 2H) The oily substance (4.30 g) was dissolved in tetrahydrofuran (8 mL), mixed with tetrabutylammonium fluoride (1 M tetrahydrofuran, 13.0 mL, 13.0 mmol) and stirred at room temperature for 3 hours. After removing the solvent by evaporation, the residue was mixed with 6 N hydrochloric acid and the thus-produced precipitate was collected by filtration, washed with hexane, and recrystallized from ethanol to give pale brown crystals (2.76 g, 15% from meldrum's acid).

1H-NMR(300MHz, CD30D)6: 2.87(dd, Jl=6.9Hz, J2=17.0Hz, 1H), 3.08(dd, Jl=3.8Hz, J2=17.0Hz, 1H), 3.72(s, 3H), 5.07(dd, J1=3.8Hz, J2=6.9Hz, 1H), 7.61(d, J=8.8Hz, 2H), 8.22(d, J=8.8Hz, 2H) Elemental analysis Found: C;53.59, H;3.93, N;4.14.

Calcd. as C15H13N08 C;53.73, H;3.9, N;4.18.

IR(KBr)1746, 1661, 1609, 1520, 1448, 1352, 1245, 1114, 1025, 884 cm- 1 To the pale brown crystals (1.00 g, 2.99 mmol), were 130 added methanol (30 mL) and distilled water (15 mL), and the mixture was mixed with lithium hydroxide (180 mg, mmol), followed by stirring at room temperature for 8 hours. After stopping the reaction by adding 1 N hydrochloric acid, methanol was removed by evaporation; the thus-obtained suspension was filtered and washed with 1 N hydrochloric acid to give pale brown crystals (901 mg, 94%).

[COMPARATIVE EXAMPLE 2] Synthesis of 4-hydroxy-6-methyl-2pyrone A solution of 1-phenyl-l-trimethylsiloxyethylene (25.0 g, 130 mmol) in absolute ether (400 mL) was cooled to -78*C, and malonyl dichloride (6.40 mL, 65.0 mmol) was added dropwise to the resulting solution by using a dropping funnel. The mixture was gradually heated to room temperature, stirred at room temperature (5 to 10*C) for 14 hours, and further stirred at 30 C for 24 hours. The thus-produced yellow precipitate was filtered and washed with absolute ether to give the target 4-hydroxy-6-methyl- 2-pyrone (8.40 g, 67%) as a yellow solid.

[EXAMPLE 122] Synthesis of Compound 122 Triethylamine (0.57 mL, 4.0 mmol) and then propionyl chloride (0.33 mL, 3.7 mmol) were added to a suspension of 4-hydroxy-6-methyl-2-pyrone (0.70 g, 3.72 mmol) in toluene mL) at room temperature. The-mixture was stirred for 131 hours as it was and then mixed with 4-N,Ndimethylaminopyridine (170 mg, 1.39 mmol), followed by heating at 90°C for 15 hours. After cooling the mixture to room temperature, insoluble substances were removed by filtration, the filtrate was washed with 5% hydrochloric acid (30 mL), and extracted four times with dichloromethane (20 mL); the organic layers were combined and dried over sodium sulfate anhydride. After concentrating the solution, the thus-obtained residue was purified by silica gel column chromatography (dichloromethane) and recrystallized from ethanol to give pale red crystals (550 mg, 61%).

[EXAMPLE 123] Synthesis of Compound 123 Similarly to Example 122, yellow crystals (1.82 g, were obtained from 4-hydroxy-6-methyl-2-pyrone (2.00 g, 15.9 mmol) and monoethyl succinyl chloride (2.20 mL, 15.6 mmol).

[EXAMPLE 124] Synthesis of Compound 124 Similarly to Example 122, yellow crystals (1.91 mg, 23%) were obtained from 4-hydroxy-6-methyl-2-pyrone (5.00 g, 39.6 mmol) and propyl chloroformate (5.4 mL, 40 mmol).

[EXAMPLE 125] Synthesis of Compound 125 Similarly to Example 122, white crystals (480 mg, were obtained from 4-hydroxy-6-methyl-2-pyrone (3.00 g, 23.8 mmol) and capryl chloride (4.9 mL, 24 mmol).

132 [EXAMPLE 126] Synthesis of Compound 126 Dichloromethane (15 mL) was added to a mixture of Compound 90 (400 mg, 1.87 mmol) and trimethyloxotetrafluoroborate (289 mg, 1.96 mmol), followed by stirring at room temperature for 21 hours.

The thus-produced precipitate was filtered and washed with dichloromethane to give white crystals (530 mg, [EXAMPLE 127] Synthesis of Compound 127 A solution of 3-carbomethoxy-4-hydroxy-6-methylpyrone (0.500 g, 2.72 mmol) in aniline (5 mL) was stirring for 2 hours while heating at 150 0 C, and then, after removing aniline by evaporation under reduced pressure, the residue was purified by silica gel column chromatography (dichloromethane) and recrystallized from ethanol to give yellow crystals (0.432 g, [EXAMPLE 128] Synthesis of Compound 128 Ethanedithiol (0.4 mL, 4.8 mmol) was added to a solution of Compound 72 (300 mg, 1.06 mmol) in trifluoroacetic acid (9 mL) at room temperature and stirred at room temperature for 2 hours. After concentrating the solution, the thus-obtained residue was mixed with 1 N hydrochloric acid and concentrated again; the thus-obtained crystals were washed with hexane and acetone to give white crystals (220 mg, [EXAMPLE 129] Synthesis of Compound 129 133.- At -78 0 C, a solution of 3-(2-methoxyphenylacetyl)-4hydroxy-6-methyl-pyrone (500 mg, 1.82 mmol) in tetrahydrofuran (10 mL) was added dropwise to a solution of lithium diisopropylamide in tetrahydrofuran (10 mL) prepared from diisopropylamine (0.56 mL, 3.98 mmol) and nbutyl lithium (2.5 M hexane solution, 1.75 mL, 4.38 mmol); the resulting mixture was stirred as it was for 30 min., heated to 0°C, stirred for further 30 min., and mixed with methyl iodide (0.27 mL, 4.3 mmol). The mixture was heated to room temperature and stirred for 2 hours; after stopping the reaction by adding 1 N hydrochloric acid mL), the water layer was extracted twice with dichloromethane (20 mL), the organic layers were combined, and dried over sodium sulfate anhydride. After concentrating the solution, the thus-obtained residue was purified by flash silica gel column chromatography (dichloromethane/hexane=l:l) and recrystallized from ethanol/hexane to give yellow crystals (150 mg, 29%).

This reaction was also carried out by using dimethyl sulfate instead of methyl iodide, and a similar result was obtained.

[EXAMPLE 130] Synthesis of Compound 130' While cooling in ice, a solution of 3-(2methoxyphenylacetyl)-4-hydroxy-6-methyl-pyrone (1.00 g, 3.65 mmol) in tetrahydrofuran (10 mL) was added dropwise 134 to a suspension of sodium hydride (0.18 g, 60% 4.4 mmol) in tetrahydrofuran (10 mL), stirred at room temperature for 1 hour, cooled to 0°C again, and mixed with n-butyl lithium (2.5 M hexane solution, 1.6 mL, 4.0 mmol). After stirring for 10 min., ethyl iodide (0.65 mL, 8.0 mmol) was added to the mixture followed by stirring for 30 min.; since red anion seeds seemed to still remain unreacted, triamide hexamethyl phosphate (1.9 mL, 21 mmol) was added to the mixture followed by stirring for 1 hour. The reaction was stopped by adding concentrated hydrochloric acid (30 mL) at 0°C and the water layer was extracted twice with dichloromethane (20 mL); the organic layers were combined and dried over sodium sulfate anhydride.

After concentrating the solution, the thus-obtained residue was purified by silica gel column chromatography (dichloromethane/hexane=2:1) and recrystallized from ethanol/hexane to give pale yellow crystals (827 mg, [EXAMPLE 131] Synthesis of Compound 131 While cooling in ice, a solution of Compound 51 (0.800 g, 3.20 mmol) in tetrahydrofuran (10 mL) was added dropwise to a suspension of sodium hydride (0.155 g, in tetrahydrofuran (15 mL), stirred at room temperature for 1 hour, cooled to 0°C again, and mixed with triamide hexamethyl phosphate (1.7 mL, 18 mmol) and then n-butyl lithium (2.5 M hexane solution, 1.4 mL, 3.5 mmol). Ten 135 min. after that, methyl iodide (0.44 mL, 7.1 mmol) was added to the mixture followed by stirring for 30 min. The reaction was stopped by adding concentrated hydrochloric acid (30 mL) at 0°C and the water layer was extracted twice with dichloromethane (20 mL); the organic layers were combined and dried over sodium sulfate anhydride.

After concentrating the solution, the thus-obtained residue was purified by silica gel column chromatography (dichloromethane/hexane=3:2) and recrystallized from ethanol/hexane to give a pale yellow oily substance (710 mg, 84%).

[EXAMPLE 132] Synthesis of Compound 132 To 11.6 g (60.3 mmol) of ethyl benzoylacetate, was added 60 mL of a 1 N aqueous sodium hydroxide solution, and the mixture was stirred at room temperature for 16 hours. After removing organic substances by ether, the reaction solution was acidified by adding concentrated hydrochloric acid while cooling the solution in an ice bath to give a precipitate. The precipitate was collected by filtration, washed with cold water, and dried under reduced pressure. As a result, 6.56 g (40.0 mmol, 66.3%) of benzoylacetic acid was obtained as a pale yellow powder.

In 40 mL of tetrahydrofuran, was dissolved 6.56 g (40.0 mmol) of benzoylacetic acid, and the resulting 136 solution was mixed with 7.13 g (44.0 mL) of carbonyldiimidazole, followed by stirring at room temperature for 2 hours. The reaction solution was acidified by adding 6 N hydrochloric acid and extracted with ethyl acetate. As a result, a large amount of insoluble crystals precipitated and were not solubilized; the crystals were concentrated under- reduced pressure and the residue was recrystallized from methanol to give 3.90 g (13.3 mmol, 66.6%) of yellow crystals.

[EXAMPLE 133] Synthesis of Compound 133 To 10.83 g (41.4 mmol) of phenylacetylmeldrum's acid, was added 70 mL of methanol, and the mixture was stirred for two and a half hours. Under reduced pressure, the reaction solution was concentrated and the residue was distilled (0.4 mmHg, 94 to 98 0 As a result, 5.96 g (31.0 mmol, 75.6%) of methyl 3-oxo-4-phenyl butyrate was obtained.

1H-NMR(300MHz, CDC1 3 3.46(s, 2H), 3.71(s, 3H), 3.83(s, 2H), 7.19-7.35(m, From 5.96 g (31.0 mmol) of methyl 3-oxo-4-phenyl butyrate, 2.73 g (8.52 mmol, 65.0%) of yellow crystals were obtained similarly to Example 132.

[EXAMPLE 134] Synthesis of Compound 134 From 10.5 g (72.8 mmol) of ethyl 3-oxopentanate, 2.72 g (13.8 mmol, 68.2%) of colorless crystals were obtained 137 similarly to Example 132.

[EXAMPLE 135] Synthesis of Compound 135 From 10.0 g (63.2 mmol) of ethyl 3-butyryl-4-hydroxy- 6-propyl-2-pyrone 3-oxohexanate, 3.86 g (17.2 mmol, 76.0%) of a pale yellow liquid was obtained by distillation (0.02 mmHg, 91 to 98*C) similarly to Example 132.

[EXAMPLE 136] Synthesis of Compound 136 From 10.0 g (63.2 mmol) of methyl 6-butyl-4-hydroxy- 6-pentanoyl-2-pyrone 3-oxoenanthate, 2.87 g (11.3 mmol, 64.0%) of pale yellow crystals were obtained similarly to Example 132.

[EXAMPLE 137] Synthesis of Compound 137 To 5.00 g (34.7 mmol) of 4-hydroxy-3-isobutyryl-6isopropyl-2-pyrone meldrum's acid, were added 20 mL of dichloromethane and 5.6 mL of pyridine, and then, 3.67 mL (34.8 mmol) of isobutyryl chloride was added dropwise to the mixture while stirring the mixture in an ice bath.

After the addition of isobutyryl chloride, the mixture was stirred for 1 hour in the ice bath, and then, stirred for further 6.5 hours at room temperature. The reaction solution was mixed with 60 mL of 2 N hydrochloric acid, extracted with dichloromethane, washed with saturated sodium chloride water, dried over sodium sulfate anhydride, filtered, and concentrated. The residue was mixed with 50 mL of methanol, heat-refluxed for 3 hours, 138and evaporated for removing the reaction solution. The residue was distilled (1.0 mmHg, 40 to 49 0 C) to give 3.08 g (21.3 mmol, 61.4%) of methyl 4-methyl 3-oxopentanate as a colorless liquid.

From 3.08 g (21.3 mmol) of methyl 4-methyl 3oxopentanate, 1.15 g (5.13 mmol, 58.0%) of a colorless liquid was obtained similarly to Example 133.

[EXAMPLE 138] Synthesis of Compound 138 From 10.0 g (69.4 mmol) of meldrum's acid and 6.25 mL (69.4 mmol) of cyclopropanecarbonyl chloride, 1.59 g (7.22 mmol, 59.8%) of colorless crystals were obtained similarly to Example 137.

[EXAMPLE 139] Synthesis of Compound 139 From 10.0 g (69.4 mmol) of meldrum's acid and 8.45 mL (69.4 mmol) of isovaleryl chloride, 2.06 g (8.16 mmol, 53.4%) of a colorless liquid was obtained by distillation (0.07 mmHg, 103 to 104*C) similarly to Example 137.

[EXAMPLE 140] Synthesis of Compound 140 With hexane, was washed several times 2.48 g (62.0 mmol) of 60% sodium hydride and dried under reduced pressure. The resultant was mixed with 100 mL of tetrahydrofuran, and then, 8.15 mL (50.0 mmol) of tertbutyl acetoacetate was added dropwise to the mixture.

The mixture was stirred at room temperature for 1 hour, and while stirring the mixture in an ice bath, 33 mL of a 139 n-butyl lithium hexane solution was added dropwise to the mixture. The mixture was stirred for 15 min. in the ice bath, and then, poured onto an excess amount of dry ice. The mixture was allowed to stand until the dry ice disappeared, and then, neutralized by adding 3 N hydrochloric acid. The resultant was extracted with ethyl acetate, dried over sodium sulfate anhydride, filtered, and concentrated. As a result, 9.71 g of an orange oily substance was obtained. The orange oily substance was mixed with 60 mL of tetrahydrofuran and then with 9.34 g (57.6 mmol) of carbonyldiimidazole. The mixture was stirred at room temperature for 17 hours. The mixture was acidified by adding 3 N hydrochloric acid, extracted with, ethyl acetate, dried over sodium sulfate anhydride, filtered, and concentrated. The residue was subjected to medium-pressure chromatography to give 3.24 g of an orange oily substance. The residue was dissolved in methanol and an aqueous sodium hydrogencarbonate solution, and then, concentrated; the residue was dissolved in water and extracted with ethyl acetate. The water layer was acidified and extracted with dichloromethane, dried over sodium sulfate anhydride, filtered, and concentrated. As a result, 423 mg (1.18 mmol) of a pale yellow oily substance was obtained.

[EXAMPLE 141] Synthesis of Compound 141 140 In 100 mL of methanol, was dissolved 5.90 g (50.0 mmol) of monomethyl malonate, and the resulting solution was mixed with 2.86 g (25 mmol) of magnesium ethoxide followed by stirring at room temperature for 4 hours. The reaction solution was concentrated and the residue was dried under reduced pressure. In 100 mL of tetrahydrofuran, was dissolved 2.80 g (25 mmol) of 2furancarboxylic acid and the resulting solution was mixed with 4.45 g (27.4 mmol) of carbonyldiimidazole, followed by stirring for 1 hour. The reaction solution was added to a dry magnesium salt and stirred at room temperature for 19 hours. The reaction solution was concentrated; the residue was mixed with 100 mL of 1.5 N hydrochloric acid, extracted with ethyl acetate, and washed with an aqueous sodium hydrogencarbonate solution and saturated sodium chloride water. The resultant was dried over sodium sulfate anhydride, filtered, and concentrated. The residue was distilled (0.06 mmHg, 69 to 75) to give 2.93 g (17.4 mmol, 69.6%) of methyl 2-furancarbonylacetate as an anhydrous liquid. Similarly to Example 133, 65 mg (1.34 mmol, 58.1%) of dark yellow crystals were obtained from 2.93 g (17.4 mmol) of methyl 2-furancarbonylacetate.

[EXAMPLE 142] Synthesis of Compound 142 In 200 mL of methanol, was dissolved 8.90 g (75.4 mmol) of monomethyl malonate, and the resulting solution 141 was mixed with 4.30 g (37.5 mmol) of magnesium ethoxide, followed by stirring for 4 hours at room temperature. The reaction solution was concentrated and the residue was dried under reduced pressure. In 150 mL of tetrahydrofuran, was dissolved 4.80 g (37.5 mmol) of 2thiophenecarboxylic acid and the resulting solution was mixed with 6.69 g (41.2 mmol) of carbonyldiimidazole, followed by stirring for 1 hour. The reaction solution was added to a dry magnesium salt and stirred at room temperature for 21 hours. The reaction solution was concentrated; the residue was mixed with 150 mL of 1.5 N hydrochloric acid, extracted with ethyl acetate, and washed with an aqueous sodium hydrogencarbonate solution and then saturated sodium chloride water. The resultant was dried over sodium sulfate anhydride, filtered, and concentrated. The residue was distilled (0.06 mmHg, 87) to give 5.95 g (32.3 mmol, 86.1%) of methyl 2thiophenecarbonylacetate as an anhydrous liquid.

Similarly to Example 133, 2.52 g (8.28 mmol, 81.2%) of yellow crystals were obtained from 5.95 g (32.2 mmol) of methyl 2-thiophenecarbonylacetate by recrystallization from methanol.

[EXAMPLE 143] Synthesis of Compound 143 From 10.0 g (69.4 mmol) of meldrum's acid and 9.45 mL (69.9 mmol) of cyclohexanecarbonyl chloride, 3.85 g (12.6 142 mmol, 63.4%) of colorless crystals were obtained by recrystallization from methanol similarly to Example 137.

[EXAMPLE 144] Synthesis of Compound 144 In 200 mL of methanol, was dissolved 8.91 g (75.4 mmol) of monomethyl malonate, and the resulting solution was mixed with 4.30 g (37.5 mmol) of magnesium ethoxide, and stirred for 4 hours at room temperature. The reaction, solution was concentrated and the residue was dried under reduced pressure. In 120 mL of tetrahydrofuran, was dissolved 4.10 mL (37.7 mmol) of cyclopentanecarboxylic acid, and the resulting solution was mixed with 6.69 g (41.2 mmol) of carbonyldiimidazole, followed by stirring hours. The reaction solution was added to a dry magnesium salt and the resulting mixture was stirred at room temperature for 221 hours. The reaction solution was concentrated; the residue was mixed with 150 mL of 2 N hydrochloric acid, extracted with ethyl acetate, and washed with an aqueous sodium hydrogencarbonate solution and then saturated sodium chloride water. The resultant was dried over sodium sulfate anhydride, filtered, and concentrated. The residue was distilled (0.15 mmHg, 51 to to give 5.72 g (33.4 mmol, 88.8%) of methyl 2cyclopentanecarbonylacetate as an anhydrous liquid.

Similarly to Example 133, 2.32 g (8.39 mmol, 57.0%) of pale yellow crystals were obtained from 5.72 g (33.4 mmol) 143 of methyl 2-cyclopentanecarbonylacetate.

[EXAMPLE 145] Synthesis of Compound 145 To 600 mg (3.63 mmol) of an oxazolopyrone derivative, was added 6 mL of 10% hydrochloric acid, followed by stirring at 90 0 C for 30 min. The reaction solution was concentrated and the residue was recrystallized from methanol. Since NMR of the thus-obtained crystals had peaks due to impurities, the crystals were recrystallized from.methanol again. As a result, 296 mg (1.61 mmol, 44.3%) of colorless crystals were obtained.

[EXAMPLE 146] Synthesis of Compound 146 While cooling in an ice bath, 37 g of concentrated sulfuric acid was added to 28 g of nitric acid, and then, 12.7 g (100 mmol) of 4-hydroxy-6-methyl-2-pyrone was slowly added to the mixture over several times. Since furious heat generation occurred during the reaction, the temperature of the reaction was controlled by using the ice bath. After the addition of the whole amount of 4hydroxy-6-methyl-2-pyrone, the mixture was stirred at to 50 0 C for 1 hour, and cooled to room temperature. The reaction solution was poured into icy water; the precipitate was filtered, washed, and recrystallized from methanol. As a result, 9.69 g (56.6 mmol, 56.6%) of a 3nitro compound was obtained as colorless crystals.

1 H-NMR(CD30D)6: 2.34(d, J=0.82, 3H), 6.25(d, J=0.82, 1H) 0 144- In 50 mL of ethanol and 35 mL of dichloromethane, was dissolved 2.09 g (12.2 mmol) of the 3-nitro compound, the resulting solution was mixed with 510 mg of 5% Pd-C (wet), followed by stirring for 7 hours in a hydrogen atmosphere.

A product was generated from the reaction solution as a precipitate. The catalyst was filtered and washed with a large amount of warm ethanol, however it was not solubilized. It was solubilized by washing with water.

The filtrate was concentrated and the residue was recrystallized from water. As a result, 1.16 g (8,22 mmol, 67.4%) of a 3-amino compound was obtained as brown crystals. The filtrate was concentrated and the residue was washed with methanol to recover 166 mg (1.2 mmol, 1 H-NMR(CD30D)6: 2.18(d, J=0.82, 3H), 5.88(d, J=0.82, 1H) In 3 mL of tetrahydrofuran, was dissolved 128 mmol (1.00 mmol) of tenoylic acid, and the resulting solution was mixed with 178 mg (1.10 mmol) of N,N'carbonyldiimidazole; after washing the wall with 1 mL of tetrahydrofuran, the mixture was stirred at room temperature for 1 hour, mixed with 141 mg (1.00 mmol) of the 3-amino compound, and stirred at room temperature for 3 hours. The 3-amino compound was not solubilized by tetrahydrofuran and it existed as a precipitate; the reaction product was suspended and was not solubilized.

145 The reaction solution became homogeneous by adding 3 N hydrochloric acid. The resultant was extracted with ethyl acetate and concentrated. The residue was recrystallized from dichloromethane/methanol to give 161 mg (0.64 mmol, 64%) of colorless crystals.

[EXAMPLE 147] Synthesis of Compound 147 In 6 mL of tetrahydrofuran, was'dissolved 224 mg (2.00 mmol) of 3-(2-furancarboamide)-4-hydroxy-6-methyl-2pyrone, 2-furancarboxylic acid, and the resulting solution was mixed with 356 mg (2.20 mmol) of N,N'carbonyldiimidazole; after washing the wall with 1 mL of tetrahydrofuran, the solution was stirred at room temperature for 0.5 hours, mixed with 282 mg (2.00 mmol) of the 3-amino compound, and stirred at room temperature for 6.5 hours. Similarly to Example 146, the reaction product was suspended and was not solubilized. The reaction solution was concentrated, mixed with 3 N hydrochloric acid, and extracted with dichloromethane.

The resultant was dried over sodium sulfate anhydride, filtered, and concentrated. The residue was recrystallized from methanol to give 319 mg (1.35 mmol, 67.5%) of pale yellow crystals.

[EXAMPLE 148] Synthesis of Compound 148 In 6 mL of tetrahydrofuran, was dissolved 246 mg (2.00 mmol) of picolinic acid, and the resulting solution 146 was mixed with 356 mg (2.20 mmol) of N,N'carbonyldiimidazole, stirred at room temperature for hours, mixed with 282 mg (2.00 mmol) of 3-amino-4-hydroxy- 2-pyrone, mixed with 4 mL of tetrahydrofuran, and stirred at room temperature for 4 hours. The reaction solution was concentrated, mixed with 3 N hydrochloric acid, and extracted with dichloromethane. The resultant was dried over sodium sulfate anhydride, filtered, and concentrated.

The residue was recrystallized from chloroform to give 295 mg (1.20 mmol; 60.0%) of pale yellow needle crystals.

[EXAMPLE 149] Synthesis of Compound 149 In 6 mL of tetrahydrofuran, was dissolved 513 mg (2.00 mmol) of palmitic acid, and the resulting solution was mixed with 356 mg (2.20 mmol) of carbonyldiimidazole, followed by stirring at room temperature for 1 hour. The reaction solution was mixed with 282 mg (2.00 mmol) of 3amino-4-hydroxy-6-methyl-2-pyrone and stirred at room temperature for 5 hours. Although the reaction solution once became homogeneous, crystals precipitated again. The reaction solution was concentrated; the residue was mixed with 3 N hydrochloric acid to filter the crystals, washed with water, and recrystallized from methanol. As a result, 574 mg (1.51 mmol, 75.5%) of the target compound was obtained as colorless crystals.

147 [EXAMPLE 150] Synthesis of Compound 150 In 100 mL of toluene, were suspended 5.6 g (30 mmol) of monoethyl pimelate, 3.8 g (30 mmol) of 4-hydroxy-6methyl-2-pyrone, 6.8 g (33 mmol) of dicyclohexanecarbodiimide, and 368 mg (3 mmol) of dimethylaminopyridine, followed by stirring at room temperature for 1 hour and at 80°C for 2 days. The resultant was filtered to remove insoluble substances and concentrated. The residue was dissolved in ethyl acetate, washed with 3 N hydrochloric acid, dried over manganese sulfate anhydride, filtered, and concentrated. The residue was purified by silica gel column chromatography to give 6.66 g (22.5 mmol, 75%) of an ethyl ester compound as an yellow oily substance.

1H-NMR(300MHz, CDCl 3 1.26(t, J=7.30, 3H), 1.42(m, 2H), 1.60-1.75(m, 4H), 2.27(d, J=0.77, 3H), 2.31(t, J=7.30, 2H), 3.08(t, J=7.30, 2H), 4.12(q, J=7.30, 2H), 5.93(d, J=0.77, 1H), 16.80(s, 1H) Using 306 mg (1.03 mmol) of the thus-obtained ethyl ester compound, preliminary investigation was made concerning hydrolysis. To the ester, was added 4 mL of a 1 N aqueous sodium hydroxide solution, and the resulting mixture was stirred for 3 days. The reaction solution was acidified by adding 3 N hydrochloric acid; the precipitate was filtered and washed with water. The resultant was 148 dried by a vacuum pump and recrystallized from ethyl acetate. As a result, 177 mg (0.66 mmol, 64%) of the target carboxylic acid was obtained as pale yellow crystals.

Similarly, 6.28 g (21.2 mmol) of the ester compound was hydrolyzed to give 4.60 g (17.1 mmol, 80.7%).

[EXAMPLE 151] Synthesis of Compound 151 Similar to Example 150, 6.81 g (21.9 mmol, 73%) of a methyl ester compound was obtained as orange crystals from 6.08 g (0.0 mmol recrystallized from 3 methanol) of monomethyl ester of azelaic acid and 3.78 g (30.0 mmol) of 4-hydroxy-6-methyl-2-pyrone by recrystallization from methanol.

1 H-NMR(300MHz, CDC1 3 1.28-1.44(m, 4H), 1.57-1.70(m, 4H), 2.27(d, J=0.67, 3H), 2.31(t, J=7.30, 2H), 3.07(t, J=7.30, 2H), 3.67(s, 3H), 5.93(d, J=0.67, 1H), 16.86(s, 1H) With 40 mL of a 1 N aqueous sodium hydroxide solution, was mixed 4.81 g (15.5 mmol) of the thusobtained methyl ester compound and the resulting mixture was stirred for 6 hours. The reaction solution was acidified by adding 3 N hydrochloric acid, extracted with dichloromethane, dried over sodium sulfate anhydride, filtered, and concentrated. The residue was recrystallized from ethyl acetate to give 3.23 g (10.9 149 mmol, 70%) of the target carboxylic acid as pale yellow crystals.

[COMPARATIVE EXAMPLE 3] Synthesis of 6-phenyl-4-oxa-dvalerolactone In an argon atmosphere, sodium hydride (5.2 g, 120 mmol) was washed with tetrahydrofuran, and to the resulting suspension in 200 mL of tetrahydrofuran, an ethyl acetoacetate solution (12.6 mL, 100 mmol) in 10 mL of tetrahydrofuran was added dropwise at room temperature.

After the addition of the ethyl acetoacetate solution, the resulting solution was stirred at room temperature for 1 hour. The solution was cooled to 0°C and butyl lithium M hexane solution, 44 mL, 110 mmol) was added dropwise to the solution;.after the addition of butyl lithium, the mixture was stirred for 20 min. as it was, and then, for further 10 min. at room temperature. The solution was cooled to 0°C again, mixed with benzaldehyde (11.0 mL, 110 mmol), and stirred at room temperature for min. By adding 5% hydrochloric acid and then concentrated hydrochloric acid, the resultant was acidified, mixed with sodium chloride, and extracted with ethyl acetate; the organic layer was dried over sodium sulfate anhydride. The solution was concentrated and the thus-obtained oily substance was mixed with a 1 N aqueous sodium hydroxide solution (200 mL) and methanol, followed 150 by stirring for 4 hours at room temperature. The reaction solution was concentrated as it was and evaporated for removing methanol, acidified by adding 5% hydrochloric acid, and extracted with dichloromethane; the organic layer was dried over sodium :sulfate anhydride. The solution was concentrated and the thus-obtained solid was recrystallized from hexane-ethyl acetate to give white crystals (14.9 g, 78%).

[EXAMPLE 152] Synthesis of Compound 152 Similarly, white crystals (44 mg, were obtained from l-oxa-2,4-oxospiro[5,5]undecane (800 mg, 4.39 mmol) and 2-thiopheneacetic acid (630 mg, 4.39 mmol).

[COMPARATIVE EXAMPLE 4] Synthesis of 6-methyl-4-oxa-dvalerolactone Pd/C (1.0 g) was added to a suspension of 4hydroxy-6-methyl-2-pyrone (10.0 g, 7.93 mmol) in ethyl acetate (35 mL) and stirred in an hydrogen atmosphere at normal pressure for 24 hours. The precipitate was filtered and the filter paper was washed with ethyl acetate and then ethanol. The thus-obtained filtrates were concentrated under reduced pressure; the thusobtained solid was recrystallized from hexane-ethyl acetate to give 6-methyl-4-oxa-6-valerolactone (74%) containing a small amount of raw material.

[EXAMPLE 153] Synthesis of Compound 153 151 To a suspension of 4-hydroxy-6-methyl-2-pyrone (1.00 g, 7.93 mmol) in toluene (30 mL), were added 4-N,Ndimethylaminopyridine (130 mg, 1.06 mmol), benzoic acid (970 mg, 7.94 mmol), and then, dicyclohexylcarbodiimide g, 9.7 mmol)' at room temperature. The mixture was stirred as it was for 10 min. and heated to 80 0 C for 17 hours. After cooling the mixture to room temperature, the thus-produced insoluble dicyclohexyl urea was removed by filtration, the reaction solution was washed with hydrochloric acid (30 mL), the water layer was extracted twice with dichloromethane (20 mL), and the organic layers were combined and dried over sodium sulfate anhydride.

After concentrating the solution, the thus-obtained residue was purified by silica gel column chromatography (dichloromethane) to give-a yellow solid (847.1 mg, 46%).

Furthermore, the solid was recrystallized from ethanol to give yellow crystals.

[EXAMPLE 154] Synthesis of Compound 154 From 4-hydroxy-6-methyl-2-pyrone (1.50 g, 11.9 mmol) and 3-cyclohexenecarboxylic acid (1.40 mL, 12.0 mmol), a yellow solid (2.40 g, 86%) was obtained similarly to Example 153. Furthermore, the solid was recrystallized from ethanol to give yellow crystals.

[EXAMPLE 155] Synthesis of Compound 155 From 4-hydroxy-6-methyl-2-pyrone (1.50 g, 11.9 mmol) 152 and cyclohexylacetic acid (1.70 g, 12.0 mmol), a yellow solid (1.80 g, 60%) was obtained similarly to Example 153.

Furthermore, the solid was recrystallized from ethanol to give white crystals.

[EXAMPLE 156] Synthesis of Compound 156 Compound 104 (1.00 g, 3.46 mmol) was mixed with tetrahydrofuran (50 mL), methanol (15 mL), concentrated hydrochloric acid (5 mL), and then, Pd-C containing 100% water, 200 mg), followed by hydrogen replacement.

Three hours later, the raw material disappeared, and hydrogen was replaced with argon. The precipitate was filtered, washed with hot methanol, and the filtrate was concentrated. The thus-obtained solid was recrystallized from ethanol/methanol to give a hydrochloride (760 mg, 74%) of the target compound.

[EXAMPLE 157] Synthesis of Compound 157 In 25 mL of chloroform, were added 3.36 g (20.0 mmol) of dehydroacetic acid and 5.68 g (20.0 mmol) of tert-butyl-4-hydroxy-benzaldehyde, and the resulting solution was mixed with 0.2 mL of piperidine and heated while being dehydrated by using a Dean-Stark dehydrating tube; since dehydration could not proceed efficiently, the solution was evaporated for removing chloroform and mixed with 25 mL of benzene for 4-hour azeotropic dehydration.

The reaction solution was cooled, concentrated, and the 153 residue was recrystallized from methanol to give 3.93 g (10.2 mmol, 51%) of the target compound as yellow crystals.

[EXAMPLE 158] Synthesis of Compound 158 From 1.68 g (10.0 mmol) of dehydroacetic acid and 1.33 mL (10.0 mmol) of 4-methylthiobenzaldehyde, 1.41 g (4.66 mmol, 46.6%) of the target compound was obtained as orange crystals by recrystallization from ethyl acetate similar to Example 157.

[EXAMPLE 159] Synthesis of Compound 159 Compound 157 (1.03 g, 2.68 mmol) was dissolved in mL of ethyl acetate, mixed with 210 mg of 5% Pd-C (wet), and stirred in a hydrogen atmosphere for 1 hour. After removing the catalyst by filtration, the filtrate was concentrated and the residue was recrystallized from methanol. As a result, 835 mg (2.16 mmol, 80.6%) of a hydrogenated compound was obtained as pale yellow crystals.

[EXAMPLE 160] Synthesis of Compound 160 With 240 mL of methanol and 50 mL of a 1 N aqueous sodium hydroxide solution, was mixed 2.36 g (5.76 mmol) of acetoxymethyl-3-palmitoyltetronic acid, and the mixture was stirred for 5 hours at room temperature. The reaction solution was concentrated; the residue was mixed with water and acidified by adding 3 N hydrochloric acid. The 154 resultant was extracted with dichloromethane, dried over sodium sulfate anhydride, filtered, and concentrated. The residue was recrystallized from methanol to give 2.03 g (5.50 mmol, 95.4%) of yellow crystals.

[EXAMPLE 161] Synthesis of Compound 161 Methanol (30 mL) and distilled water (20 mL) were added to a mixture of Compound 52 (500 mg, 2.21 mmol) and calcium carbonate (760 mg, 5.5 mmol), and stirred for hours at room temperature. After removing the stirrer, the mixture was concentrated as it was and evaporated to remove methanol. The resultant was acidified by adding hydrochloric acid, extracted three times with dichloromethane (30 mL), and the organic layers were dried over sodium sulfate anhydride. The resultant was concentrated and the thus-obtained residue was recrystallized from ethanol to give white crystals (338 mg, 83%).

[EXAMPLE 162] Synthesis of Compound 162 In 20 mL of benzene, was suspended 5.00 g (44.6 mmol) of 1,3-cyclohexanedione, and the suspension was mixed with 4.4 mL (45 mmol) of malonyl chloride, followed by heatrefluxing for 2 hours. The reaction solution was mixed with methanol to solubilize insoluble substances, mixed with silica gel, evaporated for remove the solvent, and subjected to silica gel column chromatography using ethyl 155 acetate. The thus-obtained crude product was dissolved in methanol again, mixed with silica gel, evaporated for remove the solvent, and purified by silica gel column chromatography using ethyl acetate:hexane 1:1. The thus-obtained crystals were washed with ether and filtered. As a result, 1.57 g (8.72 mmol, 19.6%) of yellow crystals were obtained.

[EXAMPLE 163] Synthesis of Compound 163 To a 100 mL, egg-plant type flask, was added 1.56 g of acetylmethyltetronic acid weighed by a balance, and mixed with 30 mL of ethanol. In 30 mL ethanol, was dissolved 0.57 g of diethoxy magnesium, and the solution was added to the flask containing acetylmethyltetronic acid. The mixture was stirred at room temperature and a solid precipitated. The mixture was filtered and dried to give 2.01 g of the target compound.

[EXAMPLE 164] Synthesis of Compound 164 To a 200 mL, egg-plant type flask, was added 1.56 g of acetylmethyltetronic acid weighed by a balance, and mixed with 30 mL of ethanol. In 120 mL methanol, was suspended 0.88- g of calcium diacetate, and the suspension was added to the flask containing acetylmethyltetronic acid. The mixture was solubilized by stirring at room temperature. The solution was concentrated and dried.

The resultant was washed with ethyl acetate and dried to 156give 1.496 g of the target compound.

[EXAMPLE 165] Synthesis of Compound 165 Compound 51 (0.50 g, 2.00 mmol) was arranged to be a sodium salt by adding mixed sodium hydrogencarbonate, and then, an aqueous solution of the sodium salt was allowed to stand at room temperature in an oxygen atmosphere for days. The solution was mixed with 1 N hydrochloric acid, extracted with dichloromethane, dried over sodium sulfate anhydride, and recrystallized from ethanol/hexane to give pale brown crystals (416 mg, 79%).

[EXAMPLE 166] Synthesis of Compound 166 Dimethylsulfoxide (5 mL) was added to a mixture of Compound 118 (1.00 g, 3.42 mmol) and N-bromosuccinimide (0.61 g, 3.4 mmol) in argon at room temperature and stirred for 20 hours. The reaction solution was mixed with distilled water and the thus-produced precipitate was recrystallized from ethanol to give pale yellow crystals (850 mg, 81%).

[EXAMPLE 167] Synthesis of Compound 167 Similarly to Example 166, pale yellow crystals (734 mg, 88) were obtained from 3-thiophenylacetyl-4-hydroxycoumarin (800 mg, 2.79 mmol).

[EXAMPLE 168] Synthesis of Compound 168 Similarly to Example 166, pale yellow crystals (240 mg, 23%) were obtained from 3-(2-methoxyphenylacetyl)- 4 157 hydroxy-6-methyl-pyrone (1.00 g, 3.65 mmol).

[EXAMPLE 169] Synthesis of Compound 169 Similarly to Example 166, pale yellow crystals (510 mg, 49%) were obtained from Compound 105 (1.00 g, 3.65 mmol).

[EXAMPLE 170] Synthesis of Compound 170 While cooling in ice, m-chloroperbenzoic acid'(300 mg, purity 80% 1.38 mmol) was added to a solution of Compound 55 (300 mg, 1.31 mmol) in dichloromethane (15 mL) and stirred for 2 hours. The reaction solution was subjected to silica gel chromatography as it was; fractions of dichloromethane/methanol (10:1) were collected and recrystallized from ethanol to give white crystals (338 mg, 83%).

[EXAMPLE 171] Synthesis of Compound 171 While cooling in ice, m-chloroperbenzoic acid (370 mg, purity 80% 1.71 mmol) was added to a solution of Compound 90 (350 mg, 1.63 mmol) in dichloromethane (15 mL) and stirred for 3 hours. The reaction solution was subjected to silica gel chromatography as it was; fractions of dichloromethane/methanol (10:1) were collected and recrystallized from ethanol to give white crystals (339 mg, 158 [COMPARATIVE EXAMPLE 5] Synthesis of 4-hydroxy-3-(l- (Compound 172) 5-(l-oxopropionyl)-2,2-dimethyl-1,3-dioxane-4,6-dione (12.15 g) and ethyl glycolate (8.25 g) were added to 25 mL of toluene and stirred at 80 0 C for 4 hours. After the completion of the reaction, toluene was removed by evaporation and the residue was purified by silica gel column chromatography (hexane:ethyl acetate= 3:1) to give 9.91 g of an acetoacetic acid derivative. The thusobtained acetoacetic acid derivative was dissolved in 31 mL of t-buthanol, mixed with 5.33 g of potassium tbuthoxide, and heat-refluxed for 4 hours. After the completion of the reaction, the reaction mixture was cooled by ice and the thus-produced precipitate was filtered. The resultant was mixed with 36.8 mmol of 1 N hydrochloric acid ethanol and tetrahydrofuran, stirred for 1 hour, and evaporated under reduced pressure for removing the solvent. The residue was distilled under reduced pressure and recrystallized from ethanol-hexane to give 3.32 g of 3-propionyltetronic acid.

[EXAMPLE 172] Synthesis of Compound 173 5-(l-oxobutyryl)-2,2-dimethyl-l,3-dioxane-4,6-dione (12.58 g) and ethyl glycolate (8.01 g) were added to 20 mL of toluene, and 9.91 g of an acetoacetic acid derivative was obtained similarly to Comparative Example 5. The 159 thus-obtained acetoacetic acid derivative was dissolved in 28 mL of t-buthanol, mixed with 5.33 g of potassium tbuthoxide, and heat-refluxed for 4 hours. After the completion of the reaction, the reaction mixture was cooled by ice and the thus-produced precipitate was filtered. The resultant was mixed with 36.8 mmol of 1 N hydrochloric acid ethanol and tetrahydrofuran, stirred for 1 hour, and evaporated under reduced pressure for removing the solvent. The residue was distilled under reduced pressure and recrystallized from ethanol-hexane to give 2.88 g of 3-butyryltetronic acid.

[EXAMPLE 173] Synthesis of Compound 174 5-(l-oxo-2-methylpropionyl)-2,2-dimethyl-l,3-dioxane- 4,6-dione (8.60 g) and ethyl glycolate (12 mL) were added to 40 mL of toluene and 7.71 g of an acetoacetic acid derivative was obtained similarly to Comparative Example The thus-obtained acetoacetic acid derivative was dissolved in 20 mL of t-buthanol and 2.55 g of 3isobutyryltetronic acid was obtained similarly to Comparative Example 1.

[EXAMPLE 174] Synthesis of Compound 175 5-(l-oxocyclopropionyl)-2,2-dimethyl-1,3-dioxane-4,6dion (17.04 g) and ethyl glycolate (10.88 g) were added to mL of toluene and 16.81 g of an acetoacetic acid derivative was obtained similarly to Comparative Example 160 The thus-obtained acetoacetic acid derivative was dissolved in 20 mL of t-buthanol and 2.32 g of 3cyclopropylcarbonyltetronic acid was obtained similarly to Comparative Example 1.

[EXAMPLE 175] Synthesis of Compound 176 Compound 43 (1.0 g) was dissolved in 100 mL of methanol, mixed with 200 mg of 5% Pd carbon, and stirred in a hydrogen atmosphere for 2 hours. After removing Pd carbon, methanol was removed by evaporation, and the residue was purified by silica gel column chromatography to give 800 mg of the target compound. The compound was arranged to be a sodium salt by adding an equivalent amount of sodium hydrogencarbonate and lyophilized.

[EXAMPLES 176 to 179] Synthesis of Compounds 177 to 180 By known methods, the above compounds were synthesized.

[EXAMPLE 180] Similarly to Example 42, Compound 181 was obtained from tetronic acid and 4-heptaenolic acid.

[EXAMPLE 181] Synthesis of Compound 182 Similarly to Example 42, Compound 182 was obtained from 5-carbomethoxymethyltetronic acid and phenacylacetic acid.

[EXAMPLE 182] Synthesis of Compound 183 Similarly to Example 42, Compound 183 was obtained 161 from tetronic acid and 4-cyclohexenecarboxylic acid.

[EXAMPLE 183] Synthesis of Compound 184 Similarly to Example 42, Compound 184 was obtained from tetronic acid and methoxyacetic acid 2thiopheneacetic acid.

[EXAMPLE 184] Synthesis of Compound 185 Similarly to Example 49, crystals (1.56 g, 60%) were obtained from 4-hydroxy-6-methyl-2-pyrone (1.26 g, 10.0 mmol) and phenoxyacetic acid (1.52 g, 10.0 mmol).

[EXAMPLE 185] Synthesis of Compound 186 Similarly to Example 49, crystals (1.09 g, 55%) were obtained from 4-hydroxy-6-methyl-2-pyrone (1.26 g, 10.0 mmol) and 2-methoxyphenylacetic acid (0.9 g, 10 mmol).

[EXAMPLE 186] Synthesis of Compound 187 Similarly to Example 49, crystals (1.86 g, 54%) were obtained from 4-hydroxy-coumarin (1.62 g, 10.0 mmol) and 2-thiopheneacetic acid (1.42 g, 10 mmol).

[EXAMPLE 187] Synthesis of Compound 188 Similarly to Example 49, crystals (1.12 g, 48%) were obtained from 4-hydroxy-coumarin (1.62 g, 10.0 mmol) and methoxyacetic acid (0.9 g, 10 mmol).

[EXAMPLE 188] Synthesis of Compound 189 Similarly to Example 49, crystals (1.16 g, 49%) were obtained from 4-hydroxy-6-methyl-2-pyrone (1.26 g, 10.0 mmol) and tetrazoleacetic acid (1.28 g, 10 mmol).

162 [EXAMPLE 189] Synthesis of Compound 190 Similarly to Example 49, crystals (1.55 g, 60%) were obtained from 4-hydroxy-6-methyl-2-pyrone (1.26 g, 10.0 mmol) and p-trylacetic acid (1.50 g, 10 mmol).

[EXAMPLE 190] Synthesis of Compound 191 Similarly to Example 49, crystals (1.62 g, 52%) were obtained from 4-hydroxy-6-methyl-2-pyrone (1.26 g, 10.0 mmol) and 2-trifluorophenylacetic acid (2.04 g, 10 mmol).

[EXAMPLE 191] Synthesis of Compound 192 Similarly to Example 49, crystals (2.53 g, 91%) were obtained from 4-hydroxy-6-methyl-2-pyrone (1.26 g, 10.0 mmol) and methyl chloroformate (0.945 g, 10 mmol).

[EXAMPLE 192] Synthesis of Compound 195 Similarly to Example 49, white crystals (1.82 g, 68%) were obtained from 2,4-dioxo-6-methyl-6-lactone (1.44 g, mmol) and 2-thiopheneacetic acid (1.42 g, 10 mmol).

[EXAMPLE 193] Synthesis of Compound 196 From 1.68 g (10.0 mmol) of dehydroacetic acid and 2thiophenecarboaldehyde (1.46 g, 10.0 mmol), 1.39 g (47%) of the target compound was obtained as orange crystals by recrystallization from ethyl acetate similarly to Example 157.

[EXAMPLE 194] Synthesis of Compound 197 From 1.68 g (10.0 mmol) of dehydroacetic acid and 3hydroxybenzaldehyde (1.22 g, 10.0 mmol), 1.55 g of 163 the target compound was obtained as orange crystals by recrystallization from ethyl acetate similarly to Example 157.

[EXAMPLE 195] Synthesis of Compound 198 From 1.68 g (10.0 mmol) of dehydroacetic acid and 2furalaldehyde (0.96 g, 10.0 mmol), 1.48 g of the target compound was obtained as orange crystals by recrystallization from ethyl acetate similarly to Example 157.

[EXAMPLE 196] Synthesis of Compound 199 From 1.68 g (10.0 mmol) of dehydroacetic acid and 2thiazolecarboaldehyde (1.13 g, 10.0 mmol), 1.21 g of the target compound was obtained as orange crystals by recrystallization from ethyl acetate similarly to Example 157.

[EXAMPLE 197] Synthesis of Compound 200 From 1.82 g (10.0 mmol) of 3-propylyl-4-hydroxy- 6 methylpyrone and 2-thiophenecarboaldehyde (1.46 g, 10.0 mmol), 1.40 g of the target compound was obtained as orange crystals by recrystallization from ethyl acetate similarly to Example 157.

[EXAMPLE 198] Synthesis of Compound 201 From 1.68 g (10.0 mmol) of dehydroacetic acid and 3,4-dihydrobenzaldehyde (1.38 g, 10.0 mmol), 1.07 g (37%) of the target compound was obtained as orange crystals by 164 recrystallization from ethyl acetate similarly to Example 157.

[EXAMPLE 199] Synthesis of Compound 202 Compound 201 (0.576 g, 2 mmol) was dissolved in 30 mL of ethyl acetate, mixed with 210 mg of 5% Pd-C (wet), and stirred for 1 hour in a hydrogen atmosphere. After removing the catalyst by filtration, the filtrate was concentrated and the residue was recrystallized from methanol. As a result, 522 mg was obtained.

[EXAMPLE 200] Synthesis of Compound 203 From 1.68 g (10.0 mmol) of dehydroacetic acid and 2nitrobenzaldehyde (1.51 g, 10.0 mmol), 2.35 g of the target compound was obtained as orange crystals by recrystallization from ethyl acetate similarly to Example 157.

[EXAMPLE 201] Synthesis of Compound 204 Compound 203 was dissolved in ethyl acetate, mixed with palladium carbon, mixed with several drops of concentrated hydrochloric acid, and stirred for 1 hour in a hydrogen atmosphere. After removing the catalyst by filtration, the filtrate was concentrated and the residue was recrystallized from methanol.

[EXAMPLE 202] Synthesis of Compound 205 Benzphenonesilyl ether was dissolved in ether and cooled to -78 C. Malonyl dichloride was added dropwise to 165 the resulting solution and stirred as it was for 5 hours.

The thus-produced precipitate was filtered and purified by column chromatography to give Compound 205.

[EXAMPLE 203] Synthesis of Compound 206 Dehydroacetic acid was dissolved in tetrahydrofuran and mixed with potassium carbonate. At room temperature, the mixture was mixed with methyl iodide and stirred as it was. The resultant was extracted with ethyl acetate and purified by column chromatography to give the target compound.

[EXAMPLE 204] Synthesis of Compound 207 Malonyl dichloride was added dropwise to a solution of acetobenzophenone in ether and stirred at room temperature. The solution was concentrated and purified by column chromatography to give Compound 207.

[EXAMPLE 205] Synthesis of Compound 208 Similarly to Example 49, white crystals (800 mg, were obtained from 4-hydroxy-6-methyl-2-pyrone (1.26 g, 10.0 mmol) and ethyl chloroformate (750 mg, 10.0 mmol).

[EXAMPLE 206] Synthesis of Compound 209 Similarly to Example 49, white crystals (800 mg, 48%) were obtained from 4-hydroxy-6-methyl-2-pyrone (1.26 g, 10.0 mmol) and methyl chloroformate (765 mg, 10.0 mmol).

[EXAMPLE 207] Synthesis of Compound 210 3-ethoxycarbonyl-4-hydroxy-6-methyl-pyridone and 166 aniline were stirred in toluene for 5 hours. The solution was concentrated and the residue was purified by column chromatography to give Compound 210.

[EXAMPLE 208] Synthesis of Compound 211 Similarly to Example 42, Compound 211 was obtained from tetronic acid and dehydrocinnamic acid.

[EXAMPLE 209] Synthesis of Compound 212 Similarly to Example 42, Compound 212 was obtained from 5,5-dimethyltetronic acid and acetic acid.

[EXAMPLE 210] Synthesis of Compound 214 Compound 212 was dissolved in ethyl acetate and stirred for 1 hour in a hydrogen atmosphere. The solution was treated similarly to Example 201 to give Compound 214.

[EXAMPLE 211] Synthesis of Compound 216 Similarly to Example 49, white crystals (1.18 g, 48%) were obtained from 4-hydroxy-6-methyl-2-pyrone (1.26 g, 10.0 mmol) and N-methyl pyrroleacetic acid (1.37 g, 10.0 mmol).

[EXAMPLE 212] Synthesis of Compound 217 Similarly to Example 49, white crystals (1.48 g, 54%) were obtained from 4-hydroxy-6-methyl-2-pyrone (1.26 g, 10.0 mmol) and a-methoxyphenylacetic acid (1.66 g, 10.0 mmol).

[EXAMPLE 213] Synthesis of Compound 218 Similarly to Example 49, white crystals (1.97 g, 54%) -167were obtained from 4-hydroxy-6-methyl-2-pyrone (1.26 g, 10.0 mmol) and palmitic acid (2.56 g, 10.0 mmol).

[EXAMPLE 214] Synthesis of Compound 219 Similarly to Example 42, Compound 219 was obtained from 5-methyltetronic acid and acetic acid.

[EXAMPLE 215] Synthesis of Compound 220 Similarly to Example 42, Compound 220 was obtained from coumarin and methoxyhydrogen glutamate.

[EXAMPLE 216] Synthesis of Compound 221 Similarly to Example 49, white crystals (1.21 g, 49%) were obtained from 4-hydroxy-6-methyl-2-pyrone (1.26 g, 10.0 mmol) and 2-pyridineacetic acid (1.37 g, 10.0 mmol).

[EXAMPLE 217] Synthesis of Compound 222 Similarly to Example 49, Compound 222 was obtained from tetronic acid and acetic acid.

[EXAMPLE 218] Synthesis of Compound 224 Similarly to Example 49, Compound 224 was obtained from 5-carbomethoxytetronic acid and acetic acid.

[EXAMPLE 219] Synthesis of Compound 225- Similarly to Example 49, Compound 225 was obtained from 5-phenyltetronic acid and acetic acid.

[EXAMPLE 220] Synthesis of Compound 227 Similarly to Example 49, Compound 227 was obtained from 5-butyltetronic acid and acetic acid.

[EXAMPLE 221] Synthesis of Compound 228 168 Similarly to Example 49, Compound 228 was obtained from tetronic acid and dodecanoic acid.

[EXAMPLE 222] Synthesis of Compound 229 Similarly to Example 49, white crystals (1.29 g, 50.0%) were obtained from 4-hydroxy-6-methyl-2-pyrone (1.26 g, 10.0 mmol) and dehydrocinnamic acid (1.50 g, 10.0 mmol).

[EXAMPLE 223] Synthesis of Compound 230- Similarly to Example 49, Compound 230 was obtained from tetronic acid and heptanoic acid.

[EXAMPLE 224] Synthesis of Compound 231 Similarly to Example 49, white crystals (2.25 g, 68%) were obtained from 4-hydroxy-6-methyl-2-pyrone (1.26 g, 10.0 mmol) and 3-nitrophenylacetic acid (1.81 g, 10.0 mmol).

[EXAMPLE 225] Synthesis of Compound 232 Compound 231 (1.00 g, 3.3 mmol) was dissolved in ethyl acetate and mixed with 100 mg of Pd/C. Several drops of concentrated hydrochloric acid was added to the mixture and the resultant was allowed to react for 5 hours in a hydrogen atmosphere. After removing the active carbon by filtration, the filtrate was concentrated to give 960 mg of the target compound.

[EXAMPLE 226] Synthesis of Compound 233 Similarly to Example 49, white crystals (1.52 g, 58%) 169 were obtained from 4-hydroxy-6-methyl-2-pyrone (1.26 g, 10.0 mmol) and 3-fluorophenylacetic acid (1.54 g, 10.0 mmol).

[EXAMPLE 227] Synthesis of Compound 235 Compound 232 (1.00 g) was dissolved in 10 mL of methylene chloride. The resulting solution was mixed with 1 mL of triethylamine and then with 920 mg of trifluoromethanesulfonate anhydride, and stirred as it was for 5 hours. The reaction solution was mixed with water and extracted with methylene chloride; the organic layer was washed three times with water and then with saturated sodium chloride water, and dried over sodium sulfate anhydride.

After removing the desiccating agent by filtration, the solution was concentrated and the residue was recrystallized from ethanol to give 1.00 g of the target compound.

[EXAMPLE 228] Synthesis of Compound 236 Similarly to Example 49, white crystals (1.44 g, 48%) were obtained from 4-hydroxy-6-methyl-2-pyrone,(1.26 g, 10.0 mmol) and 3-thianaphthalenylacetic acid (1.92 g, 10.0 mmol).

[EXAMPLE 229] Synthesis of Compound 237 From 1.68 g (10.0 mmol) of dehydroacetic acid and 1trifluorobenzaldehyde (1.74 g, 10.0 mmol), 2.52 g of 170 the target compound was obtained as orange crystals by recrystallization from ethyl acetate similarly to Example 157.

[EXAMPLE 230] Synthesis of Compound 238 From 1.68 g (10.0 mmol) of dehydroacetic acid and 2trifluorobenzaldehyde (1.74 g, 10.0 mmol), 2.43 g of the target compound was obtained as orange crystals by recrystallization from ethyl acetate similarly to Example 157.

[EXAMPLE 231] Synthesis of Compound 239 From 1.68 g (10.0 mmol) of dehydroacetic acid and 3trifluorobenzaldehyde (1.74 g, 10.0 mmol), 2.46 g of the target compound was obtained as orange crystals by recrystallization from ethyl acetate similarly to Example 157.

Structural formulae and spectral data of the above test compounds are shown below.

Compound 1 NMR (ppm) (300 MHz, CDCI3) mp. 66.5-73.51C 1.72(s, 3H), 1.98(s, 3H), 2.34(s, 3H), 2.88(dd, J= 10.43, 10.7 1, 1IH), 0O 3.16(dd, J=6.04, 7.69, 1lH), 4.41 (dd, J=6.04, 7.14, 1IH) Molecular formula CIOH13NO3S IR(cm-) (KBr) Calcd. C, 52.85; H, 5.77; N, 6.16; S, 14.11 N 0 1715, 1638, 1597, 1431, 1408, 1386, 1241, 1214, 1203, 1098, 878 Found

S

Mass (El) 227(M') Compound 2 NMR (ppm) (300 MHz, CDCI3) mp.

1.52(m, 1H), 2.04-2.23(m, 3H), 2.44(s, 3H), 3.127(m, IH), 3.74(m, IH), 3.97(dd, J=6.87, 7.00, 1H)MoeuafrmlC9 N0

HO

IR(cm'1) (neat) Calcd.

03476, 1715, 1649, 1626, 1437, 1375, 1338, 1247, 946, 739 Found Mass (El) 18 1(M+) Compound 3 NMRZ (ppm) (300 MHz, CIJC13) mp. 69-761C 1.99(m, IH), 2.18(m, 1H), 2.28-2.49(m, 2H), 2.46(s, 3H), 3.70(s, 03H), 3.88(dd, J 6.59, 1H), 6.36(br, lH) Molecular formula HO IR(cm') (KBr) Calcd. C, 52.86; H, 5.77; N, 6.16 1738, 1721, 1673, 1611, 1452, 1243, 1166, 1085 Found Me0 2 C N 0

H

Mass (El) 227(M+) Compound 4 NMR (ppm) (300 MHz, CDCI3) mp. 138-138.51C 0.96(d, J=3.30, 3H), 0.98(d, J=2.75, 3H), 1.43(m, IH), 1.74(m, 2H), Elemental analysis 2.46(s, 3H), 3.85(m, IH), 6.02(br, 1H)MoeuafrmlColNO HO IR(cm") (KBr) Calcd. C, 60.90; H, 7.67; N, 7, 1715, 1692, 1665, 1628, 1280 Found C, 60.82; H, 7.48; N, 7.15 N 0

H

Mass (El) 197(M+) Compound 5 NMR (ppm) (300 MHz, CD3OD) mp.

2,35(s, 3H), 2.70(m, IH), 3.07(m, IH), 3.79(m, 1H) 0 Molecular formula C7H8 NNaO3S NaO IR(cm') (KBr) Calcd. C, 40.19; H, 3.85; N, 6.70; S, 15.3 13 HS 1673, 1613, 1468 Found Mass Compound 6 NMR (ppm) (300 MHz, CDC13) mp. 108-11lC 2.48(dd, J=8.24, 28.29, lH), 2.47(s, 3H), 3.03(dd, J=3.02, 17.58, Elemental analysis 1H), 3.75(s, 3H), 4.14(m, 1H), 6.38(br, lH) MlclrfruaCHi 0 HO IR(cm') Calcd. C, 50.7 1; H, 5.20; N, 6.57 N72 173 165 126 12 Found C, 50.66; H, 5.24; N, 6.65

H

Mass (El) 213(M+) Compound 7 NMR (ppm) (300 MHz, CDCI3) mp. 175-176C 2.48(s, 3H), 2.83(dd, J=10.44, 14.55, lH), 3.46(dd, J=3.57, 14.83, Elemental analysis 1H), 4.12(m, lH), 5.80(br, lH), 7.06-7.24(m, 3H), 7.39(d, J=7.96, Molecular formula C15H14N203 IlH), 7.62(d, J=7.69, IlH), 8.11l(br, IlH) HO IR(cm') (KB r) Calcd. C, 66.66; H, 5.22; N 10.36 N N 0 3300, 1707, 1655, 1618, 748 Found C, 66.19; H, 5.33; N, 10. 19 Mass (El) 270(M+) Compound 8 NMR (ppm) (300 MHz, CD3OD) mp. 193-198C 1.41 J=7.14, 3H), 2.28(d, J=0.82, 3H), 4.43(q, J=7.14, 2H), Elemental analysis 5.95(d, J=0.82, lH) OH Molecular formula C9H i N04

CO

2 Et IR(cm-) (KBr Calcd. C, 54.82; H, 5.62; 7. N 0 1680, 1644, 1499, 1282, 1238 Found C, 54.36; H, 5.58; N, 7.08

H

Mass (El) 197(M+) 0 Compound 9 NMR (ppm) (300 MHz, CD3OD) mp. 89-911C 2.56(s, 0.50H), 2.58(s, 2.50H), 3.78(s, 0.33H), 3.99(s, 1.67H) Elemental analysis 0 Molecular formula C6H603S HO IR(cm') (KBr) Calcd. C, 45.56; H, 3.82; S, 20.27 1692, 1678, 1615, 1657, 1651, 1628, 1593, 1437, 1392, 1359, 1305 Found C, 45.56; H, 3.93; S, 19.74 S 0 Mass (El) 158(M+) Compound 10 NMR (ppm) (300 MHz, CDCI3) mp. 102-104'C 1.53(d, J=6.87, 3H), 4.21(s, 2H), 4.80(q, J=6.87, IH), 7.26-7.39(m, Elemental analysis Molecular formula C13HI204 HO IR(cm-') (KBr) Calcd. C, 67.23; H, 5.21 1752, 1665, 1609,;1464, 1249, 1091, 1021, 7 14 Found C, 67. 10; H, 5.35 :O 0 Mass (El) 232(M+) Compound 11 NMR (ppm) (300 MHz, CD3O1D) mp. 115-1161C 2.94(dd, J=6.04, 17.30, 1H), 3.09(dd, J=4.39, 17.30, lH), 3.69(s, Elemental analysis 3H), 3.72(m, 0.5H), 4.24(m, 0.5H), 5.09(m, 1H), 7.25-7.40(m, 5H) HO IRc')(KBr) Calcd. C, 62.07; H, 4.86 le2 1760,1734, 1661, 1618, 1448, 1390, 1249, 1238, 1178, 102 3, 719 Found C, 6'1.82; H, 4.84 Mass (El) 290(M+) Compound 12 NMR (ppm) (300 MHz, CDCI3) mp. 130-133~C 1.57(d, J=6.86, 3H), 3.95(s, 3H), 4.98(q, J6.86, 1H) Elemental analysis Molecular formnula C7H8 HO CO0 2 Me IR(cm") (KBr) Calcd. C, 48.84; H, 4.68 0 1725, 1705, 1632, 1464, 1441, 1412, 1328, 1077 Found. C, 46.64; H, 4.39 Mass (El) 172(M-) Compound 13 NMR (ppm) (300 MHz, CDCI3) mp. 122-1261C 2.78(dd, J=7.41, 16.47, lH), 3.00(dd, J=4.39, 16.74, I1H), 3.75(s, 3H), 3.96(s, Elemental analysis 3H), 5.30(dd, J=4.39, 7.69, 1H) Mlclrfnnl 610 CO2e IR(cm') (KBr) Cal .cd. C, 46.96; H, 4.3 8 MeO 2 C 01769, 1734, 1620, 1477, 1255, 1201, 1176, 1064, 801 Found C, 46.64; H, 4.39 Mass (ED) 230(M-) Compound 14 NMR, (ppm) (300 MHz, CDCI3) mp. 152-1551C 3.98(s, 3H), 5.85(s, lH), 7.35-7.45(m, 5H) Elemental analysis HO CO 2 Me Molecular formnula C12H1005 0IR(cm) (KBr) Calcd. C, 61.54; H,A430 0 1760, 1717, 1615, 1473, 1458, 1251, 1189, 1172, 1062, 1009, 702 Found C, 61.40; H, 4.3 2 Mass (El) 234(M-) Compound 15 NMR (ppm) (300 MHz, CDCl3) mp. 76-78tl 2.02(m, 1H), 2.34(m, 1H), 2.53(m, 3H), 2.56(s, 3H), 3.69(s, 1.17H), 3.70(s, Elemental analysis 1.83H), 4.71(dd, J=4.39, 8.24, 0.39H), 4.85(dd, J=4.39, 8.24, 0.61H) MlclrfruaC010 HO IR(cm'1) (KBr) Calcd. C, 52.63; H, 5.30 1765, 1738, 1667, 1622, 1346, 1168, 1023 Found C, 52.50; H, 5.32 MeO 2 C 0 Mass 228,(M-) Compound 16 NMR (ppm) (300 MHz, CD3OD) mp. 149-151LC 2.75(dd, J=7.41, 16.75, 1H), 3.05(dd, J=3.84, 16.75, 1H), 3.85(s, 3H), Elemental analysis 5.24(dd, J=3.84, 7.41, 1H) CO 2MeMolecular formula CSH807 IR(cm') (KBr) Calcd. C, 44.45; H, 3.73 MeO 2 C 1773, 1698, 1626, 1470, 1261, 1199, 1089, 1050 Found C, 44.41; H, 3.77 0Z 0 Mass (El) 216(M-) Compound 17 NMR (ppm) (300 MHz, CDCI3) mp. 112-1151C 1.57(s, 6H), 3.95(s, 3H) Elemental analysis Molecular formula C8H 1005 H;C2e IR(cmn) (KXBr Caled. C, 51.6 1; H, 5.41 "0 0 1771, 1620, 1489, 1323, 1158, 1069, 988 Found C, 5 1.61; H, 5.43 Mass (El) 1 86(M-) Compound 18 NMR (ppm) (300 MHz, CD3OD) mp. 195-200'C 2.33(s, 3H), 2.92(dd, J=7.14, 14.55, IH), 3.25(dd, J=3.84, 14.55, 1H), 0 ~4.63(m, 1H), 7.18-7.31(m, 5H) HO IR(cm-') U00r Calcd. C, 67.23; H, 5.21 I 1719, 1638, 1475 Found O 0 Mass (ED) 232(M-) Compound' 19 NMR (ppm) (300 MHz, CDC13) mp. 129-1331C 3.07(dd, J=6.04, 14.55, IH), 3.34(dd, J=4.12, 14.55, IH), 3.89(s, 3H), Elemental analysis 5.13(dd, J=4.12, 6.04, IH), 7.22-7.32(m, 5H) MlclrfruaC310 HO

CO

2 Me -IR(cm') (KBr) Calcd. C, 62.90; H, 4.87 0 01760, 1717, 1601, 1065 Found C, 62.77; H, 4.95 Mass (El) 248(M-) Compound 20 NMR (ppm) (300 MHz, CDC13) mp. l00-1l0*C 2.62(s, 3H), 7.39-7.48(m, I OH) Elemental analysis 0 Molecular formula C18H1404 HO IR(cm') (KBOr Calcd. C, 73.46; H, 4.79 Ph 1698, 1599, 1172, 977, 760 Found C, 73.42; H, 5.14 Ph 0 0 Mass (EL) 294(M-) Compound 21 NMR (ppm) (300 MHz, CD3OD) mp. 195-196C 2.42(s, 3H), 3.01(dd, J=5.49, 14.55; IH), 3.22(dd, J=4.12, 14.55, 1H), Elemental analysis 5.02(dd, J=4.39, 5.76, LH), 6.69-6.72(m, 2H), 7.04-7.07(m, 2H) MlclrfruaC310 HO 5 HO IR(cm) (KBr) Calcd. C, 62.90; H, 4.87 1 3284, 1750, 1661, 1603, 1518, 1446, 1230 Found C, 62.86; H, 4.87 0 0 Mass (El) 248(M') Compound 22 NMR (ppm) (300 MHz, CD3OD) mp. 146-1521C 2.99(dd, J=5,49, 14.83, lH), 3.27(dd, J=4.12, 14.55, IH), 3.78(s, 3H), High resolution mass spectrum 5.13(dd, J=4.12, 5.49, 1H), 6.69-6.72(m, 2H), 7.05-7.08(m, 2H) MlclrfruaC310 HOecla HO-nl CO2Me2 HO 0M IR(cm) (KBr) Calcd. 264.063 0 0 3462, 1721, 1707, 1618, 1599, 1520, 1423, 1044 Found 264.061 Mass (El) 264(M-) Compound 23 NMR (ppm) (300 MHz, CDCI3) mp.

1.99(s, 3H), 4.16-4.28(m, 3H), 4.37(br, IH), 4.56(m, 1H), 4.90(br, 1H), 7.28-7.39(m, 5H) 0 Molecular formula C15HI406 HO IR(cm') (KBr) Calcd. C, 62.07; H, 4.86 M C a n 01760, 1734, 1661, 1618, 1448, 1390, 1249, 1238, 1178, 1023, 719 Found Mass (El) 290(M-) Compound 24 NMR (ppm) (300 MHz, CDCI3) mp. 84-91 IC 1.50(d, J=7.14, 3H), 3.02(m, 2H), 3.26(m, 2H), 4.77(m, I1H), 7.19-7.39(m, Elemental analysis 0 H Molecular formula C14HI404 HO IR(cm') (KBr) Calcd. C, 68.28; H, 5.73 1746, 1663, 1607, 146.8, 1216, 1093, 700 Found C, 68.28; H, 5.75 0Z' 0 Mass (El) 246(M-) Compound 25 NMR (ppm) (300 MHz, CD3OD) mp. 54-57*C 2.89(dd, J=6.32, 11.03, IH), 2.99-3. 10(m, 3H), 3.24(m, 2H), 5.05(dd, J=4.39, Elemental analysis 0 1H), 7.21-7.32(m, 5H) HO IR(cm') (KBr Caled, C, 63.15; H, 5.30 MeO 2 C 0 1754, 1736, 1661, 1611, 1274, 1174, 1025 Found C, 63.16; H, 5-26 Mass (El) 304(M) Compound 26 NMR (ppm) (300 MHz, CD3OD) mp. 103-1041C 2.90(dd, J=6.32, 17.02, 1H), 3.08(dd, J=4.12, 17.02, IH), 3.71, 3.73(s, 3H), Elemental analysis 5.04, 5.12(m, 1H), 6.86-7.05(m, 2H), 7.25-7.33(m, lH)MoeuafrmlC3H2S HO IR(cm- 1 (KBr) Calcd. C, 52.70; H, 4.08; S, 10.82

S

MeO 2 C a O1756, 1734, 1659, 1613, 1454, 1245, 1178, 1021, 719 Found C, 52.70; H, 4.05; S, 10.75 O0 Mass (El) 296(M') Compound 27 NMR (ppm) (300 MHz, CD3OD) mp.

J=0.55, 6.86, 6H), 2.95(dd, J=6.32, 17.30, LH), 3.09(dd, J=4.12, 017,30, lH), 3.70(m, lH),3.72(s, 3H), 5.09(dd, J=4.12, 6.04, IH) Molecular formula CiiH1406 HO IR(cm") (KBr) Calcd. 242.079 W 2:)Y,1771, 1744, 1696, 1603, 1330, 1226, 1178, 1087, 1021 Found 242.080 O 0 Mass (ES) 242(M-) Compound 28 NMR (ppm) (300 MHz, CD3OD) mp. 126-128t.

1.12-2.05(m, 11H), 3.85(s, 3H), 4.81(d, J3.30, 1H) Elemental analysis HO CO 2 Me Molecular formula C12HI605 IR(cm') (KBr) Calcd. C, 59.99; H, 6.71 0 1754, 1717, 1601, 1477, 1259, 1207, 1170, 1058, 1011 Found C, 59.90; H, 6.68 Mass (CI) 241 (M+H)Y Compound 29 NMR (ppm) (300 MHz, CD3OD) mp. 92-951C 01. 13-2.05(m, 1 IlH), 2.53(s, 3H), 4.68(d, J=3.84, IlH) Elemental analysis HOMolecular formula C 12 H 604 IR~m)(Kr)Calcd. C, 64.27; H, 7. 19 O 0 1750, 1663, 1599, 1456, 1156, 1048, 1011, 404 Found C, 64.33; H, 7.09 Mass (El) 224(M-) Compound 30 NMR (ppm) (300 MHz, CD3O1D) mp. 1l0.5-112.5'C 2.92(dd, J=6.59, 17.02, 1IH), 3.l11(dd, J=4.12, 17.02, 1IH), 3.75(s, 3H), Elemental analysis 5.19(dd, J=4.12, 6.59, IH), 7.29-7.46(m, 4H) HO IR(cm-') (KBr) Calcd. C, 55.48; H, 4.04; Cl, 10.92 1765, 1738, 1661, 1604, 1027 Found C, 55.34; H, 4.05; Cl, 11.09 Me0 2 C CI 0o 0 Mass (El) 324(M-) Compound 31 NMR (ppm) (300 MHz, CD3OD) mp.

2.02(quint, J=7.14,'2H), 2.47(t, J=7.14, 2H), 2.88-3.12(m, 4H), 3.70(s, 3H), 0 3H), 5.08(dd, J=4.12, 6.31, I H)Moeua n-u C360 HO

C

2 Me IR(cm) (neat) Calcd. C, 52.00; H, 5.37 MeO2C 0 1769, 1742, 1700, 1605, 1439, 1377, 1019Fon Mass (El) 300(M-) Compound 32 NMR (ppm) (300 MHz, CD3OD) mp. 87-901C 2.02(quint, J=7.14, 2H), 2.47(t, J=7.14, 2H), 3.03(t, J=7.14, 2H), 1.69(s, Elemental analysis 0 3H), 5.83(s, 1H), 7.38-7.47(m, Molecular formula C16HI606 IR(cm") (KBr) Calcd. C, 63.15; H, 5.30 0 01742, 1651, 1609, 1170 Found C, 63.14; H, 5.29 Mass (El) 304(M') Compound 33 NMR (ppm) (300 MHz, CD3OD) mp. 80-8l 0

C

1.22(t, J=7.14, 3H), 2.94(dd, J=5.77, 1.7.02, 1 3.04(dd, J=4.39,,17.02, Elemental analysis U 0 IH), 4.10-4.26(m, 3H), 5.08(m, IH), 7.28-7.39(m, 5H) Molecular fo mula C16H1606 HO IR(cm) (KBr) Calcd. C, 63.15; H, 5.30 E2C1748, 1725, 1661, 1247, 1187, 1019 Found C, 63.16; H, 5.34 EtO 2 0 Mass (El) 304(M-) Compound 34 NMR (ppm) (300 MHz, CD3OD) mp. 108-111 0

IC

3.86(s, 3H), 4.25(d, J=3.84, IH), 4.71(d, J=1.92, lH), 5.21 J=1.92, 1 Elemental analysis OO 0 .9735m H Molecular formula C15HI407 HOIR(cm') (KBr) Calcd. C, 58.83; H, 4.61 MeO2C 01771, 1742, 1649, 1620, 1122,988,727 Found C, 58.72; H, 4.70 OH Mass (El) 306(M') Compound 35 NMR (ppm) (300 MHz, CD3O1D) mrp. 1.53(m, 2H), 1.63(m, 2H), 2.22(t, J=7.14, 2H), 2.38(t, J=7.14, 2H), 2.58(dd, Elemental analysis J=8.51, 16.20, 1 3.02(dd, J=3.57, 16.20), 3.69(d, J=0.82, 3H), 3.75(d, J=0.82, 3H), 5.11 J=3.57, 8.5 1) Molecular formula C 13 HI807 HO GO 2 Me (KBr) Calcd. C, 54.54; H, 6.34 Me02 0 7214,17,13,17,19 Found C, 54.60; H, 6.32 Mass (El) 286(M-) Compound 36 NMR (ppm) (300 MHz, CD3OD) mp. 86-881C 2.02(m, IH), 2.3 1(m, IH), 2.52(t, J=7.14, 2H), 3.70(s, 3H), 4.20(m, LH), Elemental analysis 0 7.27-7.37(m, 5H) HO IR(cnf') (K.Br Calcd. 63.15; H, 5.30 1744, 1661, 1609, 1444, 1245, 1027, 729, 712 Found C, 63.15; H, 5.3.0 MeO2C 0 0 Mass (ED) 304(M) Compound 37 NMR (ppm) (300 MI-z, CD3OD) mp. 128-1321C 2.41-2.52(m, 3H), 2.80(t, J=7.69, 2H), 2.94(dd, J=3.30, 16.20), 3.75(s, 3H), Elemental analysis 5.06(dd, J=3.30, 8.78), 7.19-7.32(m, 5H) MlclrfruaC HO IR(cm"') (KBr) Caled. C, 65.21; H,,5.84 7 1740, 1711, 1632, 1276 Found C, 65.07; H, 5.84 MeO 2 Ca n 0 0 Mass (El) 276(M-) Compound 38 NMR (ppm) (300 MHz, CD3OD) mp. 140-1441C 2.92(dd, J=6.31, 17.30, IH), 3.09(dd, J=4.39, 17.30, 1H), 3.71(s, 3H), Elemental analysis 3.72(m, I 5.09(dd, J=4.39, 6.3 1, 1 7.26-7.44(m, 4H) MlclrfruaC51C0 CIMoeua o-uaC53C0 HO IR.cm.' (KBr) Calcd. C, 55.48; H, 4.04; Cl, 10.92' 1740, 1661, 1609, 1444, 1243, 1021 Found C, 55.42; H, 4.07; Cl, 10.95 Me0 2

C

Mass (El) 324(M-) Compound 39 NMR (ppm) (300 MHz, CD3OD) mp. 104-1071C 2.94(dd, J=6.31, 17.30, 1H), 3.08(dd, J=4.39, 17.30, 1H), 3.69(s, 3H), 3.80(s, Elemental analysis 3H), 5.07(dd, J=4.30, 6.3 1, 1 6.88-6.92(m, 2H), 7.28-7.3 1(m, 2H) We Molecular formula C16HI607 O zzt Cac.O,6.0;H,50 JR(cm') (KBr) MeO2C -0 1748, 1729, 1661, 1613, 1518, 1439, 1247 Found C, 60.06; H, 5.03 Mass (El) 320(M-) Compound 40 NMR (ppm) (300 MHz, CD3OD) mp. 82-831C 0.94(t, J=6.87, 3H),l.27-l.40(m, 27H), 1.72(m, 2H), 2.04(s, 3H), 2.95(t, Elemental analysis J=7.14, 2H), 4.39(dd, J=3.84, 12.35, IH), 4.58(dd, J=2.74, 12.35, 1H), 0 5.03(dd, J=2.74, 3.83; 1IH) Molecular formula C23H3806 HO (H14H Rc KOCalcd. C, 67.29; H, 9.33 AcO a ,2920, 2852, 1750, 1665, 1613 Found C, 67.28; H, 9.32 O 0 Mass (El) 410O(M-) Compound 41 NMR (ppm) (300 MHz, CD30D) mp. 79-80°C 0.94(t, J=6.87, 3H),1.28-1.40(m, 27H), 1.73(m, 2H), 2.90-2.98(m, 3H), Elemental analysis 3.08(dd, J=4.39, 17.30, 1H), 3.72(s, 3H), 5.04(dd, J=4.39, 6.21, 1H) M 0 Molecular formula C23H3806 HO (CH,)1 IR(cm-') (KBr) Calcd. C, 67.29; H, 9.33 MeO 2 C O n 2920, 2852, 1752, 1731, 1620 Found C, 67.33; H, 9.31 Mass (El) 410(M*) Compound 42 NMR (ppm) (300 MHz, CD30D) mp. 111.0-112.0 C 1.62-1.79 1H), 1.91-2.06(m, 1H), 2.14-2.37 4H), 3.62-3.69 1H), Elemental analysis 4.76 2H), 5.76-5.77 IH).

0 Molecular formula Ci 1 H1204 HO IR(cm') (KBr) Calcd. C, 63.45; H, 5.81.

3028, 2928, 1750, 1661, 1601, 1466, 1433, 1348, 1263, 1251, 1205, 1116, Found C, 63.41; H, 5.93.

O O 1048, 843, 830, 644 Mass (El) 208 Compound 43 NMR (ppm) (300 MHz, CD30D) mp. 51.0-53.0 C 2.00 (quint, J=7.4 Hz, 2H), 2.46 J=7.4 Hz, 2H), 2.97 J=7.4 Hz, 2H), Elemental analysis 3.70 2H), 4.74 2H). Molecular formula C O Molecular formula CioHlo06 HO CO 2 Me IR(cm (KBr) Calcd. C, 52.63; H, 5.30.

3174, 3146, 2950, 1781, 1740, 1661, 1615, 1460, 1441, 1390, 1305, 1249, Found C, 52.56; H, 5.38.

O O 1207, 1125, 1054, 1017, 841,766 Mass (EI) 228 Compound 44 NMR (ppm) (300 MHz, CD30D) mp. 107.0-109.0 C 4.76 2H), 4.91 2H), 6.96-7.02 2H), 7.29-7.32 1H). Elemental analysis O Molecular formula CIOH804S HO IR(cm (KBr) Calcd. C, 53.56; H, 3.60; S, 14.30.

3110, 1748, 1663, 1607, 1466, 1431, 1352, 1257, 121.8, 1100, 1042, 948, Found C, 53.61; H, 3.65; S, 14.10.

O 0 857, 694 Mass (EI) 224 Compound 45 NMR (ppm) (300Q MHz, CD3OD) mp. 70.5-7 1.0 'C 4.75 2H), 4.91 2H), 7.07-7. 10 (in, I 7.28-7.30 (in, I 7.37-7.40 Elemental analysis, 0 s Molecular formnula CioH8O4S HO IR(cm') (KBr Calcd. C, 53.56; H, 3.60; S, 14.30.

3108, 1752, 1698, 1667, 1603, 1466, 1431, 1255, 1214, 1102, 1044, 1023, Found C, 53.62; H, 3.62; S, 14.05.

O 0 837, 752 Mass (El) 224 Compound 46 NMR (ppm) (300 MHz, CDC13) mp. 145 0 C(0.2 mmHg) 1.56(d, 3=6.87, 1.59H), 1.60(d, J=6.87, 1.41 4.76(q, J=6.87, 0.47H), 4.92(q, J=6.87,.0.53H), 7.51l-7.57(m, 3H), 7.66(m, IH), 8.27-8.34(m, 2H) 0 Molecular formnula C12HI004 HO IR(cm) (neat) Calcd. C, 66.05; H, 4.62 1767, 1684, 1595, 1560, 1450, 1052, 851, 775, 698, 600 Found O 0 Mass (El) 218(M') Compound 47 NMR (ppm) (300 MHz, CDCl3) mp.

l.28(t, 3=7.14, 3H), l.41(t, 3=7.14, 3H), 2.76(dd, J=7.41, 16.48, IH), 2.98(dd, J=4.39, 16.48, 1lH), 4.2 1(q, J=7.14, 2H), 4.43(q, J=7.14, 2H), Mlclroml ~110 HO0

CO

2 Et 5.29(m, IH)MoeuafomlC IR(cm-) (neat) Calcd. C, 51.16; H, 5.46 EtO 2 C 1760, 1734, 1452, 1381, 1187, 1038, 806 Found 0 0 Mass 258(M-) Compound 49 NMR (ppm) (300 MHz, CD3OD) inp. 104.0-106.0 'C 1. 17 3=7.14 Hz, 3H), 2.32 J=0.82 Hz, 3H), 3. 10 J=7.14 Hz, 2H), 6.18 3=0.82 Hz, 1H).

OH 0 Molecular formula IR(cin) Calcd.

I I-Found JO 0 Mass 182 Compound 50 NMR (ppm) (300 MHz, CD30D) mp. 72.5-73.0 °C 2.32 J=0.82 Hz, 3H), 2.34-2.48 2H), 3.16-3.20 2H), 4.66-5.14 Elemental analysis OH 2H), 5.86-6.00 1H),6.19 J=0.82 Hz, IH). Molecular formula Ci H1204 OH O Molecular formula C i i H1204 SIR(cm-) (KBr) Calcd. C, 63.45; H, 5.81.

3084, 1715, 1651, 1609, 1570, 1456, 1390, 1344, 1236, 996, 940, 919, 861, Found C, 63.48; H, 5.87.

O 0 712 Mass (EI) 208 Compound 51 NMR (ppm) (300 MHz, CD30D) mp. 126.0-127.5 "C 2.33 J=0.82 Hz, 3H), 4.61 2H), 6.20 J=0.82 Hz, 1H), 6.95-7.00 Elemental analysis OH 2H), 7.30-7.33 1H). Molecular formula CI2HIoO4S SIR(cm (KBr) Calcd. C, 57.59; H, 4.03; S, 12.81.

IR(cm (KBr) S 3082, 1711, 1626, 1533, 1454, 1369, 1321, 1238, 1170, 990, 932, 855, 795, Found C, 57.57; H, 4.04; S, 12.72.

772,721,712 O O Mass (EI) 250 Compound 52 NMR (ppm) (300 MHz, CD30D) mp. 91.0-93.5 °C 2.34 J=0.82 Hz, 3H), 3.51 3H), 4.73 2H), 6.24 J=0.82 Hz, 1H). Elemental analysis OH O Molecular formula C9H00o5 OMe IR(cm" (KBr) Calcd. C, 54.54; H, 5.08.

1750, 1736, 1638, 1578, 1460, 1342, 1137, 996, 984, 940, 919, 845 Found C, 54.62; H, 5.11.

O 0O Mass (EI) 198 Compound 53 NMR (ppm) (300 MHz, CD30D) mp. 78-79°C 1.91-2.05(m, 4H), 2.40(t, J=7.41, 2H), 2.4-2.9(br, 4H), 3.1(t, J=7.41, 2H) Elemental analysis OH O Molecular formula Cl H1405

CO

2 H IR(cm) (KBr) Calcd. C, 58.40; H, 6.24 1723, 1632, 1560, 1446, 1423, 1274, 1197, 1168 Found C, 58.34; H, 6.25 Mass 226(M Mass (EI) 226(M-) 0 Compound 54 NMR (ppm) (300 MHz, CD30D) mp. 137-139C 2.32(d, J=0.82, 3H), 4.40(s, 2H), 6.19(d, J=0.82, 1H), 7.27-7.35(m, 4H) Elemental analysis COH Molecular formula CI4HI1 C104 SIR(cm (Kr) Calcd. C, 60.34; H, 3.98; Cl, 12.72 1742, 1719, 1638, 1615, 1570, 996, 779 Found C, 60.22; H, 4.04; Cl, 12.66 O O Mass (EI) 278(M*) Compound 55 NMR (ppm) (300 MHz, CD30D) mp. 80.0-81.0 °C 2.16 3H), 2.33 J=0.82 Hz, 3H), 2.83 J=7.14 Hz, 2H), 3.39 Elemental analysis J=7.14 Hz, 2H), 6.20 J=0.82 Hz, 1H).

OH O Molecular formula CIOH1204S SMe IR(cm 1 (KBr) Calcd. C, 52.61; H, 5.30; S, 14.05.

1715, 1647, 1560, 1460, 1421, 1247, 998, 973, 942, 847 Found C, 52.45; H, 5.33; S, 13.84.

O 0 Mass (EI) 228 Compound 56 NMR (ppm) (300 MHz, CD30D) mp. 91.0-92.5 IC (dec) 2.37 J=0.82 Hz, 3H), 6.33 J=0.82 Hz, 1H), 7.54 IH). Elemental analysis OH O Molecular formula C8H6C1204 CI IR(cm") (KBr) Calcd. C, 40.54; H, 2.55; Cl, 29.91.

1711, 1647, 1562, 1450, 1253, 1000, 837, 810, 770, 752 Found C, 40.62; H, 2.63; Cl, 29.82.

0- 0 Mass (EI) 236 Compound 57 NMR (ppm) (300 MHz, CD30D) mp. 128.5-130.0 °C 1.85-2.03 3H), 2.34 J=0.82 Hz, 3H), 2.44-2.62 1H), 3.94-4.16 Elemental analysis OH in 2H), 5.47-5.52 IH), 6.23 J=0.82 Hz, 1H). Molecular foula C OH O Molecular formula Ci I H1205 SIR(cm (KBr) Calcd. C, 58.92; H, 5.40.

2984, 2878, 1725, 1642, 1620, 1564, 1460, 1238, 1098, 1083, 1000, 880, Found C, 58.88; H, 5.46.

O O 849 Mass (EI) 224 0 Compound 58 NMR (ppm) (300 MHz, CD30D) mp. 66.0-66.5 IC 0.98-1.22 5H), 1.63-1.83 5H), 1.93 1H), 2.32 J=0.82 Hz, Elemental analysis 3H), 2.95 J=6.59 Hz, 2H), 6.17 J=0.82 Hz, 1H).

OH 0 Molecular formula CI4HI804 OH O IR(cm) (KBr) Calcd. C, 67.18; H, 7.25.

2926, 2854, 1725, 1651, 1611, 1562, 1448, 1340, 1249, 1236, 996, 932, 859 Found C, 67.17; H, 7.24.

OO

Mass (EI) 250 Compound 59 NMR (ppm) (300 MHz, CD30D) mp. 57.0-57.5 °C 0.97-1.15 2H), 1.56-1.75 4H), 1.83-1.98 2H), 2.32 J=0.82 Elemental analysis Hz, 3H), 2.36 (sept, J=0.82 Hz, 1H), 3.10 J=7.14 Hz, 2H), 6.17 J=0.82 OH O Hz, 1H). Molecular formula C13HI604 IR(cm-) (KBr) Calcd. C, 66.08; H, 6.83.

3082, 2954, 2870, 1717, 1649, 1613, 1566, 1454, 1354, 1238, 996, 936, Found C, 66.04; H, 6.80.

S O 866,775,716 Mass (EI) 236 Compound 60 NMR (ppm) (300 MHz, CD30D) mp. 148-149 C 2.32 J=0.82 Hz, 3H), 4.41 2H), 6.18 J=0.82 Hz, 1H), 7.22-7.39 Elemental analysis S5H Molecular formula C14H1204 OH O IR(cm (KBr) Calcd. C, 68.84; H, 4.95.

3082, 3034, 2932, 1707, 1655, 1560, 1460, 1238, 1170, 998, 946, 862, 727, Found C, 68.90; H, 5.02.

702, 542 Mass (EI) 244 Compound 61 NMR (ppm) (300 MHz, CD30D) mp. 59.0-59.5 *C 1.03 J=7.42 Hz, 3H), 1.72 (sext, J=7.42 Hz, 2H), 2.32 J=0.82 Hz, 3H), Elemental analysis OH O 3.05 J=7.42 Hz, 2H), 6.18 J=0.82 Hz, 1H). Molecularformula CloHI204 IR(cm (KBr) Calcd. C, 61.21; H, 6.17.

3078, 2974, 2942, 2916, 2882, 1725, 1655, 1613, 1557, 1462, 1381, 1348, Found C, 61.18; H, 6.13.

O O 1238,994,920,861,777 Mass (EI) 196 Compound 62 NMR (ppm) (300 MHz, CD30D) mp. 75.0-75.5 °C 0.99 J=7.42 Hz, 3H), 1.44 (sext, J=7.42 Hz, 2H), 1.67 (quint, J=7.42 Hz, Elemental analysis 2H), 2.32 J=0.82 Hz, 3H), 3.07 J=7.42 Hz, 2H), 6.18 J=0.82 Hz, OH O 1H). Molecular formula C l H1404 IR(cm (KBr) Calcd. C, 62.84; H, 6.71.

3090, 2964, 2874, 1719, 1653, 1609, 1564, 1454, 1361, 1332, 1234, 1189, Found C, 62.77; H, 6.63.

O 0 996,934,861,772,714,640 Mass (EI) 210 Compound 63 NMR (ppm) (300 MHz, CD30D) mp. 91.5-92.0 °C 2.34 J=0.82 Hz, 3H), 3.75 3H), 4.02 2H), 6.25 J=0.82 Hz, 1H). Elemental analysis OH O O Molecular formula CIOHo006 OMe IR(cm) (KBr) Calcd. C, 53.10; H, 4.46.

1717, 1640, 1618, 1572, 1448, 1386, 1299, 1251, 1158, 998, 988,944, 777 Found C, 53.04; H, 4.47.

O

Mass (EI) 226 Compound 64 NMR (ppm) (300 MHz, CD30D) mp. 74.5-75.0 C 1.18 J=6.73 Hz, 6H), 2.32 J=0.82 Hz, 3H), 3.94 (sept, J=6.73 Hz, 1H), Elemental analysis OH 0 6.18(q,J=0.82Hz, Molecular formula C1oHI204 SIR(cm (KBr) Calcd. C, 61.21; H, 6.17.

1721, 1647, 1611, 1562, 1458, 1425, 1365, 1236, 1000, 982, 930, 859 Found C, 61.06; H, 6.09.

S 0O Mass (EI) 196 Compound 65 NMR (ppm) (300 MHz, CD30D) mp. 134.5-135.5 'C 2.32 J=0.82 Hz, 3H), 2.49 3H, SMe), 4.37 2H), 6.19 J=0.82 Hz, Elemental analysis SMe 1H) 724 (brs 4H)Molecular formula C15H1404S OH O IR(cm (KBr) Calcd. C, 62.05; H, 4.86; S, 11.05.

1709, 1651, 1560, 1497, 1460, 1238, 996, 948, 936, 862, 777 Found C, 61.83; H, 4.87; S, 11.05.

O 0 Mass (EI) 290 Compound 66 NMVR (ppm) (300 MHz, CD3OD) mp. 194.5-195.5 IC 2.38 J=0.82 Hz, 3H), 5.14 2H), 6.27 J=0.82 Hz, 1H), 7.87-7.98 (in, Elemental analysis 04H). Molecular formula C 13 H i i N06 OH 0 N IR(cnf') (KBr) Calcd. C, 61.34; H, 3.54; N, 4.47.

1730, 1715, 1638, 1620, 1458, 1419, 1406, 1110, 994, 949, 721, 530, 514 Found C, 61.59; H, 3.65; N, 4.46.

O 0 0 Mass (EL) 313 Compound 67 NMR (ppm) (300 MHz, CD3OD) mp. 41-431C 1.11 l(d, J=6.31, 3H), 1. 14(t, J=7.14, 3H), 2.26(m, 2H), 2.54(m, 2H), 2.75(m, Elemental analysis 3.05(dq, J=0.82, 7.14, 2H), 7.36-7.39(m, 2H)MoeuafrmlCoH40 IR(cm) (KBr) Calcd. C, 65.92; H, 7.74 1657, 1555, 1543 Found C, 65.92; Hj, 7.76 0 Mass (El) 1 82(M-) Compound 68 NMR (ppm) (300 MHz, CD3OD) mp. 43-44 0

C

1. 12(d, J=6.04, 3H), 2.26(m, 2H), 2.57(m, 2H), 2.58(s, 3H), 2.75(m, I Elemental analysis OH 0 ~~3.05(dq, J=0.82, 7.14,12H), 7.36-7.39(m, 2H) Mlclrfnnl IR(cm) (KBr Calcd. C, 64.27; H, 7.19 1642, 1562, 1458 Found C, 64.20; H, 7,15 0 Mass (El) 168(M') Compound 69 NMR (ppm) (300 MHz, CD3OD) mp. 54-55r- 1. 12(d, J=6.31, 3H), 2.26(m, 2H), 2.57(m, 2H), 2.75(m, 1lH), 4.91 IlH), Elemental analysis 6.95-6.98(m, 2H), 7.29(dd, J=2.47, 3.84, 1H) OH O0 Molecular formnula C13HI403S IR(cnf') (KBr Calcd. C, 62.38;.H, 5.64; S, 12.81 S 1665, 1607, 1562, 1423, 1404, 698 Found C, 62.22; H, 5.68; S, 12.76 0 Mass (EL) 250(M-) Compound 70 NMR (ppm) (300 MHz, CD30D) mp. 83.0-83.5 C 2.05 (quint, J=7.30 Hz, 2H), 2.32 J=0.82 Hz, 3H), 2.95 J=7.30 Hz, Elemental analysis 2H),3.14 J=7.30 Hz, 2H), 6.18 J=0.82 Hz, 1H), 6.85-6.87 1H), M f H 0 6.92-6.95 1H), 7.19-7.22 1H). Molecular formula C4H404S R(cm) (KBr) alcd. C, 60.41; H, 5.07; S, 11.52.

IR(cm-') (KBr) S 1725, 1649, 1620, 1560, 1460, 1433, 1334, 1249, 996, 924, 851,688 Found C, 60.40; H, 5.06; S, 11.48.

O 0 Mass (El) 278 Compound 71 NMR (ppm) (300 MHz, CD30D) mp. 128-130 C 1.52-1.68 1H), 1.94-2.05 IH), 2.14-2.31 4H), 2.33 J=0.82 Elemental analysis OH Hz, 3H), 3.84-3.95 1H), 5.75-5.77 2H), 6.19 J=0.82 Hz, 1H). Molecular foula C7H SIR(cm-) (KBr) Calcd. C, 59.14; H, 7.09.

2986, 2936, 2674, 2598, 1650, 1555, 1323, 1272, 1110, 1008, 911 Found C, 59.16; H, 7.02.

O 0 Mass (EI) 142 Compound 72 NMR (ppm) (300 MHz, CD30D) mp. 106-109 C (dec) 1.50 3H), 2.34 J=0.82 Hz, 3H), 4.49 2H), 6.23 J=0.82 Hz, 1H) Elemental analysis OH 0 Molecular formula C13HI706N

NHCO

2 Bu t IR(cm) (KBr) Calcd. C, 55.12; H, 6.05; N, 4.95.

3332, 2982, 1720, 1675, 1654, 1563, 1527, 1456, 1301, 1250, 1238, 1185, Found C, 55.23; H, 6.04; N, 5.08.

0 O 1164,995,938,857 Mass (EI) 283 Compound 73 NMR (ppm) (300 MHz, CD30D) mp. 135-136 °C 2.76 3H), 7.37-7.41 1H), 7.80-7.86 1H), 7.80-7.86 IH), Elemental analysis 8.11-8.15 1H).

OH 8.11-8.15 Molecular formula C I H804 S IR(cm (KB) Calcd. C, 64.70; H, 3.95.

IR(cm (KBr) 1734, 1612, 1545, 1498, 1444, 1370, 1170, 1034, 981,764, 579 Found C, 64.54; H, 4.04.

s Mass (EI) 204 Compound 74 NMR (ppm) (300 MHz, CD3OD) mp. 85.0-85.5 00 1.47 6H), 2.89 2H), 4.58 2H), 6.69-7.02 (in, 2H), 7.30-7.33 (in, Elemental analysis I H).

O H 0 1Molecular formula C1 3 HI404S S Rc" Kr Calcd. C, 58.63; H, 5.30; S, 12,04.

1692, 1572, 1543, 1311, 1067, 940, 768, 721 Found C, 58.32; H, 5.3 0; S, 11.97.

O 0 Mass (El) 266 Compound 75 NMR (ppm) (300 MHz, CD3OD) mp.

1.12(d, J=6.04, 3H), 2.28(mn, 2H), 2.53(mn, 2H), 2.77(m, 1H), 3.48(s, 3H), High resolution mass spectrum OH 0 .0dJ 0 H Molecular formula Ci0H1404 W~e IR(ci') Calcd. 198.089 Found 198.09 1 0 Mass (EL) 198(M-) Compound 77 NMR (ppm) (300 MHz, CD3OD) inp.

1. 14(t, J=7.41, 3H), 2.35(s, 3H), 2.52(q, J=7.41, 2H), 2.65(s, 3H), 5.44(s, High resolution mass spectrum 1H) OH 0 Molecular formula Ci0H1204 IR(cmn) Calcd. 196.074 Found 196.075 O 0 Mass (El) 196(M') Compound 78 NMR (ppm) (300 MHz, CD3OD) mp.

1. 14(t, J=7.41, 3H), 1. 17(t, J=7.41, 3H), 2.35(s, 3H), 2.52(q, J=7.41, 2H), High resolution mass spectrum 3.11 J=7.41, 2H) OH 0 Molecular formula Cii H1404 IR(cm) Calcd. 2 10.089 Found 210.090 O 0 Mass (El) 196(M') Compound 79 NMR (ppm) (300 MHz, CD30D) mp. 105-160 C 1.49 6H), 2.61(s, 3H), 2.87 2H). Elemental analysis OH O Molecular formula C9H1204 IR(cm (KBr) Calcd. C, 58.68; H, 6.57.

2984, 1710, 1562, 1465, 1414, 1256, 1181, 1064, 935, 842, 769 Found C,.58.52; H, 6.48.

O 0 Mass (EI) 184(M') Compound 80 NMR (ppm) (300 MHz, CD30D) mp. 174-175 'C 2.33 J=0.82 Hz, 3H), 2.50 3H), 4.48 2H), 6.23 J=0.82 Hz, IH), Elemental analysis Me 7.47 J=8.34 Hz, 2H), 7.84 J=8.34 Hz, 2H). Molecular formula Cl5H1406S O H IR(cm) (KBr Calcd. C, 55.89; H, 4.38; S, 9.95.

0* 1IR(cm (KBr) O 2920, 1704, 1653, 1550, 1323, 1244, 1160, 1129, 997, 887, 814 Found C, 55.51; H, 4.41; S, 9.84.

O O Mass (El) 322 Compound 81 NMR (ppm) (300 MHz, CD30D) mp. 160-161 °C 2.34 J=0.82 Hz, 3H), 3.61 (dd, J=17.85 Hz, J'=7.96 Hz, 1H), 3.81 (dd, Elemental analysis J=17.85 Hz, J'=4.39 Hz, 1H), 6.15 (dd, J=7.96 Hz, J'=4.39 Hz, 1H), 6.25 (q, OH 0 0 J=0.82 Hz, 1H), 7.63-7.94 4H). Molecular formula C6H206 IIR(cm) (KBr) Calcd. C, 63.99; H, 4.03.

IR(cm (KBr) 1757, 1739, 1641, 1566, 1458, 1296, 1215, 1069, 1001, 835 Found C, 63.67; H, 4.12.

S 0O Mass (EI) 300 Compound 82 NMR (ppm) (300 MHz, CD30D) mp. 78-79 °C 1.52-1.68 1H), 1.94-2.05 1H), 2.14-2.31 4H), 2.33 J=0.82 Elemental analysis OH O Hz, 3H), 3.84-3.95 1H), 5.75-5.77 2H), 6.19 J=0.82 Hz, IH). Molecular formula C 404 IR(cm' (KBr) Calcd. C, 66.65; H, 6.02.

3096, 3026, 2916, 2838, 1713, 1647, 1655, 1455, 1352, 1236, 998, 867 Found C, 66.77; H, 6.02.

OMs 023 Mass (EI) 234 Compound 83 NMR (ppm) (300 MHz, CD30D) mp.

1.14(t, J=7.41, 3H), 2.36(s, 3H), 4.63(s, 1.5H), 6.98-7.00(m, 2H), 7.31(m, High resolution mass spectrum

IH)

OH O Molecular formula C14H1404S N. IR(cm-) (neat) Calcd. 278.061 S 1725, 1638, 1607, 1553, 1431 Found 278.064 O 0 Mass (EI) 278(M*) Compound 84 NMR (ppm) (300 MHz, CD30D) mp. 160-1631C 2.33(s, 3H), 2.51(s, 3H), 5.46(s, 1H) Elemental analysis O OH Molecular formula C8H804 IR(cm) (KBr) Calcd. C, 57.14; H, 4.80 1688, 1663, 1615, 1599, 1545, 1493, 1357, 1278 Found C, 57.01; H, 4.81 O 0 Mass (EI) 168(M') Compound 85 NMR (ppm) (300 MHz, CD30D) mp. 55-57°C 2.39(s, 3H), 2.55(s, 3H), 2.69(s, 3H) O OH O Molecular formula CloHloOs IR(cm (KBr) Calcd. C, 57.14; H, 4.80 1744, 1705, 1665, 1618, 1562, 1402 Found O 0 Mass (EI) 210(M') Compound 86 NMR (ppm) (300 MHz, CD30D) mp. 64-66 °C 1.34-1.53 1H), 1.53-1.86 7H), 1.87-2.00 2H), 2.61 3H), 2.84 Elemental analysis OH 0 2H). Molecular formula C12H1604 IR(cm (KBr) Calcd. C, 64.27; H, 7.19.

S2952, 2870, 1707, 1560, 1464, 1415, 1238, 1077, 1054, 762 Found C, 64.28; H, 7.18.

Mass (EI) 224 (M) Compound 87 NMR (ppm) (300 MHz, CD30D) mp. 65-67 °C 1.93-2.18 2H), 2.71-2.98 4H), 4.41-4.56 1H), 4.57 2H), Elemental analysis 6.97-7.03 2H), 7.20-7.35 1H+5H).

OH O Molecular formula C19HI804S IR(cm-) (KBr) Calcd. C, 66.64; H, 5.30; S, 9.37.

S 3090, 3032, 2926, 1714, 1573, 1433, 1283, 1265, 1076, 943, 908, 713, 698 Found C, 66.54; H, 5.31; S, 9.27.

PhO 0O Mass (EI) 342 Compound 88 NMR (ppm) (300 MHz, CD30D) mp. 104-104.5 C 2.35 3H), 6.28 IH), 6.39 1H), 7.99-8.01 1H), 8.06-8.08 Elemental analysis SH. Molecular formula C I H804S S IR(cm") (KBr) Calcd. C, 55.92; H, 3.41; S, 13.57.

3086, 1751, 1650, 1574, 1522, 1248, 1225, 1162, 1056, 736 Found C, 55.83; H, 3.49; S, 13.53.

O O Mass (El) 236 Compound 89 NMR (ppm) (300 MHz, CD30D) mp. 119.7-120.1'C 2.33(3H, d, J=0.8Hz), 4.45(1H, s, enolic proton), 6.20(1H, 6.28(1H,m), 6.38(IH,m), 7.45(1H, q, J=0.82Hz) OH O Molecular formula Cl2HoO05 OC IR(cm.) (KBr) Calcd. C, 70.06; H, 6.61 1773, 1752, 1657, 1613, 1460, 1249, 1127, 1052, 1017, 830 Found C, 69.93; H, 6.65 O 0 Mass Compound 90 NMR (ppm) (300 MHz, CD30D) mp. 78-79 °C 2.14 3H), 2.34 J=0.82 Hz, 3H), 3.85 2H), 6.22 J=0.82 Hz, 1H). Elemental analysis OH O Molecular formula C9H1004S SMe IR(cm) (KBr) Calcd. C, 50.45; H, 4.71; S, 14.97.

1711, 1651, 1562, 1460, 996, 862, 528 Found C, 50.39; H, 4.66; S, 14.79.

S(

Mass (El) 214 Compound 91 NMR (ppm) (300 MHz, CD30D) mp. 62-62.5 'C 1.46 J=7.14 Hz, 3H), 2.26 J=0.82 Hz, 3H), 5.27 J=7.16 Hz, 1H), Elemental analysis OH 6.14 J=0.82 Hz, 1H), 7.20-7.37 5H). Molecular formula C15H404 IR(cm) (KBr) Calcd. C, 69.75; H, 5.46.

3096, 3030,2986, 2940, 1717, 1651, 1560, 1460, 1241, 1009, 998, 864, Found C, 69.71; H, 5.51.

O 752, 700 Mass (EI) 258 Compound 92 NMR (ppm) (300 MHz, CD30D) mp. 84-85 C 0.91-0.97 (br m, 3H), 1.28-1.44 (br m, 14H), 1.62-1.84 (br 2.32 Elemental analysis J=0.82 Hz, 3H), 3.09 J=7.14 Hz, 2H), 6.18 J=0.82 Hz, 1H).

OH O Molecular formula C17H2604

S(CH

2 )gCH Calcd. C, 69.36; H, 8.90.

3090, 2960, 2924, 2854, 1719, 1653, 1611, 1557, 1458, 1249, 996, 930 Found C, 69.38; H, 8.99.

O 0 Mass (EI) 294 Compound 93 NMR (ppm) (300 MHz, CD30D) mp. 83-84 C 2.11-2.24 1H), 2.27-2.38 1H), 2.33 J=0.82 Hz, 3H), 3.81-4.08 Elemental analysis OH O 4H), 4.33-4.42 IH), 6.21 J=0.82 Hz, IH). Molecularformula Cl H1205 SIR( (KBr) Calcd. C, 58.92; H, 5:40.

3047, 2956, 2872, 1723, 1644, 1611, 1564, 1454, 1238, 1064, 998 Found C, 58.95; H, 5.41.

O 0 Mass (EI) 224 Compound 94 NMR (ppm) (300 MHz, CD30D) mp. 89-90 'C 0.91-0.97 (br m, 3H), 1.28-1.44 (br m, 26H), 1.62-1.84 (br m, 2.32 Elemental analysis OH J=0.82 Hz, 3H), 3.07 J=7.14 Hz, 2H), 6.18 J=0.82 Hz, IH). Molecular formula C22H604 OH O Molecular formula C22H3604

(CH

2 14 CH IR(c (KB) Calcd. C, 72.49; H, 9.96.

2918, 2854, 1719, 1655, 1562, 1473, 1245, 994, 716 Found C, 72.47; H, 9.95.

OMa (0l) Mass (EI) 364 Compound 95 NMR (ppm) (300 MHz, CDC13) mp. 109-110 C 0.85-0.91 (br m, 3H), 1.24-1.43 (br m, 14H), 1.63-1.77 (br m, 2H), 3.20 Elemental analysis J=7.42 Hz, 2H), 7.29-7.37 2H), 7.66-7.72 1H), 8.05-8.09 1H).

OH O Molecular formula C19H2404

S(CH

2 8 CHz IR(cm') (KBr) Calcd. C, 72.12; H, 7.64.

2924, 2858, 1719, 1609, 1549, 1433, 1228, 1201, 1031, 980, 899 Found C, 72.08; H, 7.68.

O O Mass (EI) 316 Compound 96 NMR (ppm) (300 MHz, CDC13) mp. 112-114 C 0.85-0.91 (br m, 3H), 1.24-1.46 (br m, 26H), 1.63-1.77 (br m, 2H), 3.20 Elemental analysis J=7.42 Hz, 2H), 7.29-7.37 2H), 7.66-7.72 1H), 8.05-8.09 1H).

OH O Molecular formula C25H3604 (C'e14C~ e IR(cm (KBr)

(CH

2 14 CH. IR(cm (KB) Calcd. C, 74.96; H, 9.06.

2956, 2920, 2856, 1715, 1607, 1551, 1437, 1230, 1031, 982, 899, 766 Found C, 74.98; H, 9.09.

Mass (El) 400 Compound 97 NMR(ppm) (300 MHz, CD3OD) mp. 172-173 C 2.35 J=0.82 Hz, 3H), 4.66 2H), 6.21 J=0.82 Hz, 1H), 7.36-7.39 Elemental analysis OH S 1H), 7.59 IH), 7.84-7.91 2H). Molecular formula CI6HI CIO4S IR(cm-') (KBr) Calcd. C, 57.40; H, 3.31; Cl, 10.59; S, 9.58.

3096, 1717, 1636, 1618, 1572, 1450, 1419, 1077, 996, 942, 830, 787 Found C, 57.08; H, 3.50; Cl, 10.69; S, 9.56.

0 0 l Mass (El) 334 Compound 98 NMR (ppm) (300 MHz, CD30D) mp. 161-162'C 2.31 J=0.82 Hz, 3H), 4.51 2H), 6.15 J=0.82 Hz, 1H), 7.00-7.15 Elemental analysis 2H), 7.26 1H), 7.35-7.38 1H), 7.58-7.61 1H).

OH O NH Molecular formula C16H13N04 SIR(cm (KBr) Calcd. C, 67.84; H, 4.63; N, 4.95.

3376, 3084, 1715, 1644, 1607, 1553, 1452, 1238, 1098, 996, 942, 855, 737 Found C, 67.50; H, 4.63; N, 4.95.

O 0 Mass (EI) 283 Compound 99 NMR (ppm) (300 MHz, CD3OD) .mp. 84-85 'C 2.35 J=0.82 Hz, 3H), 4.34 2H), 6.22 J=0.82 Hz, lH), 7.26-7.33 (in, Elemental analysis OHd 3H), 7.43-7.46 (mn, 2H). Molecular formula C14HI204S SPh IR(c' (KBr) Calcd. C, 60.85; H, 4.38; S, 11.61.

1717, 1636, 1618, 1564, 1454, 1390, 1168, 994, 938, 743 Found C, 60.85; H, 4.5 1; S, 11.55.

O 0 Mass (El) 276 Compound 100 NMR (ppm) (300 MHz, CD3O1D) mp. 155-156 IC 2.35 J=0.82 Hz, 3H), 2.39 (in, 3H), 4.71 2H), 6.22 J=0.82 Hz, 1H), Elemental analysis OH 07.29-7.42 (in, 2H), 7.71-7.74 (in, 1H), 7.78-7.82 (in, lH). Molecular formula Ci7HI404S

(CH

2 8 CH,- IR(cin) (KBr) Calcd. C, 64.95; H, 4.49; S, 10.20.

1744, 160.5 9 5 5 4 0 4 1 2 7 0 0 3 8 6 5 Found C, 64. 84; H, 4.5 3; S, 10.20.

O1 0 Mass (EL) 314 Compound 101 NMR (ppm) (300 MHz, CDC13) mp. 85.7-87.2tC.

0.93(3H, t, J=6.6Hz), 1.33(broad s, 14H), 1.65(2H, quint, J=7.4Hz), 2.84(2H, t, J=7.4Hz), 4.46(2H, s) O Molecular formula Ci5 H2404 HO. IRc" KOCalcd. C, 67.14,; H, 9.01

(CH

2 )gCH 3 IRc) Kr Z )1773, 1750, 1663, 1615 Found 0 0 Mass (EL) 268(M+) Compound 102 NMR (ppm) (300 MHz, CDCI3) inp. 79.7-80.3'C 0.94(3H, t, J=6.6Hz), l.33(IOH, broad 1.63(2H, quint, J=7.4Hz),-2.82(2H, 0 J=7.4Hz), 4.35(2H, s) MlclrfruaC320 HO (H)C3 Icm1 KOCalcd. C, 64.98; H, 8.39 1773, 1748, 1661, 1615 Found C, 65.20; H, 8.60 0 Mass (El) 240(M+) Compound 103 NMR (ppm) (300 MHz, CDC13) mp. 68.9-69.5°C 0.94(3H, t, J=6.86Hz), 1.42-1.34(m, 8H), 1.7(2H, quint, J=7.4Hz), 2.84(2H, Elemental analysis t, J=7.4Hz), 4.72(2H, s) 0 t, s) Molecular formula C12H1804 HO (CH26CH3 IR(cm (KBr) Calcd. C, 63.70; H, 8.02 1773, 1750, 1661, 1615 Found C, 63.68; H, 7.97 0O Mass (El) 226(M+) Compound 104 NMR (ppm) (300 MHz, CD30D) mp. 202-203 'C 2.34 J=0.82 Hz, 3H), 4.58 2H), 6.22 J=0.82 Hz, 1H), 7.56 J=8.2 Elemental analysis NO Hz, 2H), 8.23 J=8.2 Hz, 2H). Molecula formula C4H OH N2 Molecular formula CI4Hil N06 OH O SIR(cm (KBr) Calcd. C, 58.13; H, 3.83; N, 4.84.

1721, 1640, 1607, 1562, 1518, 1348, 996, 938,830, 737 Found C, 57.79; H, 3.94; N, 4.83.

O 0 Mass (EI) 289 Compound 105 NMR (ppm) (300 MHz, CD30D) mp. 109-110 C 2.32 J=0.82 Hz, 3H), 3.80 3H), 4.33 2H), 6.18 J=0.82 Hz, 1H), Elemental analysis OH e 6.88 J=8.8 Hz, 2H), 7.24 J=8.8 Hz, 2H).

SOMe Molecular formula C15HI405 OH O IR(cm-) (KBr) Calcd. C, 65.69; H, 5.15.

3082,2930, 1702, 1651, 1615, 1553, 1518, 1458, 1301, 1257, 1183, 1033, Found C, 65.66; H, 5.18.

o s 994,946,791 Mass (EI) 274 Compound 106 NMR (ppm) (300 MHz, CD30D) mp. 133-134'C 2.25 3H), 2.33 J=0.82 Hz, 3H), 2.88 J=6.3 Hz, 2H), 3.34 J=6.3 Elemental analysis ~OH ~0 ~Hz, 2H), 6.19 J=0.82 Hz, 1H). Molecular foula 205 OH O Molecular formula CI 1H1205 IR(cm (KBr) Calcd. C, 58.92; H, 5.40.

3102, 2922, 1711, 1638, 1624, 1553, 1446, 1427, 1373, 1241, 1168, 994, Found C, 58.85; H, 5.35.

S 0 0 924, 855 Mass (EI) 224 Compound 107 NMR (ppm) (300 MHz, CD3OD) mp. 159-160 0

C

2.33 J=0.82 Hz, 3H), 4.40 2H), 6.20 J=0.82 Hz, IH), 7.03-7.09 (in, Elemental analysis O 0IF 2H), 7.30-7.34 (in, 2H). Molecular formula C14Hi 1F04 K~r)Calcd. C, 64.12; H, 4.23.

1738, 1653, 1607, 1564, 1514, 1462, 1319, 1218, 992, 928, 845, 793 Found C, 63.93; H, 4.27.

Mass (ES) 262 Compound 108 NMR (ppm) (300 MHz, CDCl3) mp. 51.5-521C 0.95(3H, t, J=7.1Hz), 1.47(4H, in), 1.85(2H, quint, J=7.4Hz), 2.17(2H, in), 2.27(2H, mn), 3.03(2H, t, J=7. 1Hz), 4.74(2H, s) MlclrfruaC410 HO IR(cm') (KBr) Calcd. C, 67.18; H, 7,25 01773, 1752, 1657, 1607, 404 Found Mass Compound 109 NMR (ppm) (300 MHz, CDCl3) mp. 86.2-87.31C 1.23 1l(d,J=6.86,1.8H), l.234(d,J=6.86,4.2H), 2.88(sept, J=6.86, IH), 2.98 Elemental analysis J=7.96, 2H), 3.24(t, 1=7.96, 2H), 4.53(s, 0.60H),4.53(s, l.40H),7.17(s, 4H) 0 Molecular formula Ci 6H 1804 HO Ici)(r)Calcd. C, 70.06; H, 6.61 on" 1773, 1752, 1657, 1613, 1460, 1249, 1127, 1052, 1017, 830 Found C, 69.93; H, 6.65 0 Mass (El) 274(M') Compound 110 NMR (ppm) (300 MHz, CDCl3) mp. 82.1-82.91C 0.95(d, J=6.59, 6H), l.66(dd, J=13.59, 2H), 1.81 (sept,J=6.59, 1H), Elemental analysis 3.96(t,J=6.59, 21H), 4.13(s, 2H), 4.58(s, 0.6H),4.67(s, 1.4H), 6.86(d, J=8.5l1, 6H), 7.26(d, J=12.96, 2H) Molecular formula Cl 7H2005 0 HO IR(cm') (KBr) Calcd. C, 67.09; H,6.62 1752, 1671, 1605, 1510, 1388, 1286, 1249, 1178, 1040, 1013, 982,8 870, 795 Found C, 66.92; H, 6.6 4 0 0 Mass (ED) 304(M+) Compound 111 NMR (ppm) (300 MHz, CD30D) mp. 158-159 °C 2.31 J=0.82 Hz, 3H), 2.93 6H), 4.28 2H), 6.16 J=0.82 Hz, 1H), Elemental analysis 6.77 J=8.8 Hz, 2H), 7.17 J=8.8 Hz, 2H).

O NMe2 Molecular formula Cl6Hl7N04 OH O M IR(cm-) KBr) Calcd. C, 66.88; H, 5.96; N, 4.88.

1711, 1655, 1620, 1562, 1531, 1462, 1359, 1238, 1170, 996, 783 Found C, 66.70; H, 5.96; N, 4.98.

O 0 Mass (EI) 287 Compound 112 NMR (ppm) (300 MHz, CD30D) mp. 161-162 C 2.33 J=0.82 Hz, 3H), 4.52 2H), 6.21 J=0.82 Hz, 1H), 7.50 J=8.8 Elemental analysis Hz, 2H), 7.64 J=8.8 Hz, 2H). Molecular formula C5 F304 C Molecular formula Ci 5 HiI F304 OH O IR(cm (KBr) Calcd. C, 57.70; H, 3.55.

1719, 1638, 1574, 1421, 1332, 1160, 1116, 1069, 998, 830 Found C, 57.54; H, 3.58.

S 0O Mass (EI) 312 Compound 113 NMR (ppm) (300 MHz, CD30D) mp. 196-197 C 2.33 J=0.82 Hz, 3H), 3.95 J=22.8 Hz, 2H), 6.21 J=0.82 Hz, 1H). Elemental analysis OH O O Molecular formula C9H0 O7P P^ H IR(cm) (KBr) Calcd. C, 38.73; H, 3.66.

P'OH IR(cm (KBr) 2920, 2236, 1723, 1680, 1642, 1603, 1557, 1458, 1241, 1218, 1129, 1023, Found C, 38.50; H, 3.64.

O O 1006, 951 Mass (EI) 248 Compound 114 NMR (ppm) (300 MHz, CD30D) mp. 83-84 °C 0.94 (br t, J=6.8 Hz, 3H), 1.27-1.47 (br m, 10H), 1.68 (br m, 2H), 2.32 Elemental analysis J=0.82 Hz, 3H), 3.06 J=7.4 Hz, 2H), 4.28 2H), 6.18 J=0.82 Hz, OH O IH). Molecular formula C15H2204

(CH

2 7 CH IR(cm (KBr) Calcd. C, 67.64; H, 8.33.

3090, 2962, 2969, 2856, 1715, 1557, 1618, 1564, 1452, 1236, 1183,996 Found C, 67.59; H, 8.18.

O

Mass (El) 266 Compound 115 NMR (ppm) (300 MHz, CDCI3) mp. 84.9-86.5'C 0.90-0.94(m, 3H), 1.36-1.44(m, 4H), 1.75-1.79(m, 2H), 3.91-3.95 Elemental analysis (in, 2H), 2H), 4.58(s, 0.6H), 4.67(s, 1.40H), 6.84-6.86(m, 2H), 0 7.26-7.29(m, 2H) Molecular formula Ci 17 H20 HO IR(cm-') (KBr) Calcd. C, 67.09; H,6.62 1752, 1671, 1657, 1609, 1514, 1421, 1390, 1253, 1180, 1038, 876, 801 Found C, 67.00; H,6.54 Mass (El) 304(M+) Compound 116 NMR (ppm) (300 MHz, CDCI3) mp.

0.86-0.93(m, 12H), 1.88-2.1ll(m, 4H), 2.42-2.49(m, 3H), 2.99-3.22( High resolution mass spectrum 0 0 m5H,46(,I)Molecular formula Ci7 H27 NO IR(cm') (KBr) Calcd. 325.189 O 1738, 1719, 1638, 1620, 1562, 1460, 1286, 1255, 1234, 849, 681, 629 Found 325.190 Mass (El) 325(M+) Compound 117 NMR (ppmn) (300 MHz, CDCI3) mnp. 80.6-81.4'C 1.24(d, J=6.87, 6H), 2.27(s, 3H), 2.89(sept, J=6.87, 1H), 2.93(t, J=7.41, Elemental analysis OH 02H), 3.41 J=7.41, 2H), 5.94(s, IH), 7.14-7.26(m, 4H) Molecular formula Ci8H2004 (KBr) Calcd. C, 71.98; H, 6.71 /1738, 1719, 1642, 1609, 1562, 980, 932, 833, 822 Found C, 71.81; H,.6.66 O 0 Mass (El) 300(M+) Compound 118 NMR (ppm) (300 MHz, CD3OD) mp. 122-123 2.35 3H), 4.49 2H), 7.14-7.24 (in, 4H), 7.38-7.48 7i80-7.86 Elemental analysis H) 8. 1-814 IH).Molecular formula Ct8H1404 OH 0 O IR(cm-') (KBr) Calcd. C, 73.46; H, 4.80.

I1729, 1622, 1549, 1508, 1444, 1185, 1029, 984, 758 Found C, 73.25; H, 4.76.

.0 0 Mass (El) 294 Compound 119 NMR (ppm) (300 MHz, CD30D) mp. 170-172 °C 2.37 J=0.82 Hz, 3H), 4.84 2H), 6.24 J=0.82 Hz, IH), 7.47-7.51 Elemental analysis 1H), 7.55-7.61 1H), 7.69-7.75 1H), 8.16-8.19 1H).

OH O Molecular formula C 14H1 lNO6 SIR(cm (KBr) Calcd. C, 58.15; H, 3.96; N, 5.09.

1721, 1653, 1624, 1562, 1520, 1456, 1350, 1317, 994, 731 Found C, 58.13; H, 3.83; N, 4.84.

NO2 0 0 Mass (EI) 289 Compound 120 NMR (ppm) (300 MHz, CD30D) mp. 187-188 C 2.32 J=0.82 Hz, 3H), 4.32 2H), 5.95 2H), 6.19 J=0.82 Hz, 1H), Elemental analysis 6.77 (br s, 1H), 6.78 IH), 6.82 (br s, 1H).

O- Molecular formula C15Hl206 OH O r S IR(cm (KBr) Calcd. C, 62.49; H, 4.20.

3082, 2930, 1707, 1651, 1622, 1560, 1497, 1450, 1259, 1040, 996, 944, Found C, 62.37; H, 4.24.

L 928, 793 0 0 Mass (EI) 288 Compound 121 NMR (ppm) (300 MHz, CDCI3) mp. 194-196 C 2.84 (dd, Jl=6.6 Hz, J2=17.3 Hz, 1H), 3.05 (dd, J1=3.8 Hz, J2=17.3 Hz, 1H), Elemental analysis 5.06 (dd, J1=3.8 Hz, J2=6.6 Hz, IH), 7.61 J=8.8 Hz, 2H), 8.22 J=8.8

NO

2 2H). Molecular formula C14H I NOs O Hz, 2H).

HO I IR(cm) (KBr Calcd. C, 81.92; H, 3.52; N, 4.28.

H

2 C 3278, 1748, 1659, 1605, 1514, 1352, 1241, 1170, 1021 Found C, 52.34; H, 3.45; N, 4.36.

0 Mass (EI) 321 Compound 122 NMR (ppm) (300 MHz, CD30D) mp. 153-155 C 1.21 J=7.14 Hz, 3H), 3.17 J=7.14 Hz, 2H), 6.93 2H), 7.58-7.63 Elemental analysis OH O 3H), 8.00-8.04 2H). Mo r f a Molecular formula C14Hl1204 IR(cm') (KBr) Calcd. C, 68.84; H, 4.95.

O O 1722, 1632, 1558, 1437, 1240, 1057, 887, 773, 685 Found C, 68.67; H, 4.99.

Mass (EI) 244 Compound 123 NMR (ppm) (300 MHz, CD30D) mp. 80.5-81.0 C 1.28 J=7.14 Hz, 3H), 2.33 J=0.82 Hz, 3H), 2.69 J=6.32 Hz, 2H), Elemental analysis 3.40 J=6.32 Hz, 2H), 4.17 J=7.14 Hz, 2H), 6.20 J=0.82 Hz, 1H).

OH O Molecular formula C12H1406 OEt IR(cm 1 (KBr) Calcd. C, 56.69; H, 5.55.

1734, 1719, 1651, 1562, 1180, 1156, 998, 861,806 Found C, 56.52; H, 5.50.

o 0 O Mass (EI) 254 (M Compound 124 NMR (ppm) (300 MHz, CD30D) mp. 64-68 C 1.06 J=7.14 Hz, 3H), 1.82 (qd, J1 7.14 Hz, J2 6.59 Hz, 2H), 2.32 Elemental analysis ~OH O J=0.82 Hz, 3H), 4.34 J=6.59 Hz, 2H), 6.21 J=0.82 Hz, 1H). M OH O Molecular formula CioHI205 O IR(cm (KBr) Calcd. C, 56.60;.H, 5.70.

3100, 2976, 2884, 1742, 1651, 1570, 1423, 1354, 1274, 1102, 996 Found C, 57.32; H, 5.66.

O 0 Mass (EI) 212 Compound 125 NMR (ppm) (300 MHz, CD30D) mp. 73-74 °C 0.91-0.97 (br m, 3H), 1.28-1.44 (br m, 12H), 1.62-1.84 (br m, 2H), 2.32 Elemental analysis ~OH J=0.82 Hz, 3H), 3.06 J=7.14 Hz, 2H), 6.18 J=0.82 Hz, 1H). Molecular formula C6H2404 OH O Molecular formula C 16H2404

(CH

2 8 CH IR(cm (KBr) Calcd. C, 68.54; H, 8.63.

2954, 2918, 2854, 1720, 1656, 1562, 1474, 1460, 996 Found C, 68.44; H, 8.65.

O 0 Mass (EI) 280 Compound 126 NMR (ppm) (300 MHz, CD30D) mp. 154-161 C (dec) 2.39 J=0.82 Hz, 3H), 3.03 6H), 6.33 J=0.82 Hz, 1H). Elemental analysis OH O Molecular formula CIoHi3BF404S SMe 2 IR(cm- (KBr) Calcd. C, 38.00; H, 4.15; S, 10.15.

BF

4 3046, 2994, 2974, 2928, 2880, 1740, 1721, 1642, 1576, 1452, 1259, 1083, Found C, 37.89; H, 4.15; S, 10.40.

O O 1036, 988 Mass (FAB) 230 Compound 127 NMR (ppm) (300 MHz, CD30D) mp. 155-158 "C 2.35 J=0.82 Hz, 3H), 6.27 J=0.82 Hz, 1H), 7.18-7.23 1H), Elemental analysis 7.37-7.43 2H), 7.64-7.67 2H).

OH O Molecular formula CI3HII NO4 IR(cm-) (KBr) Calcd. C, 63.67; H, 4.52; N, 5.71.

N

H 3070, 1707, 1603, 1547, 1450, 1251, 998, 965, 754 Found C, 63.68; H, 4.58; N, 5.93.

O 0 Mass (EI) 245 Compound 128 NMR (ppm) (300 MHz, CD30D) mp. 210-230 C (dec) 2.38 (br s, 3H), 4.48 2H), 6.32 (br s, 1H). Elemental analysis OH O Molecular formula C8HioCINO4

NH

3 Cl- IR(cm') (KBr) Calcd. C, 43.75; H, 4.59; N, 6.38; Cl, 16.14.

2970, 2840, 1709, 1640, 1557, 1417, 1272, 1228, 1170, 996, 862 Found C, 43.63; H, 4.61; N, 6.36; Cl, 16.22.

O 0 Mass (FAB) 185 Compound 129 NMR (ppm) (300 MHz, CD30D) mp. 125-126 C 1.44 J=6.87 Hz, 3H), 2.29 J=0.82 Hz, 3H), 3.81 3H), 5.35 Elemental analysis J=6.87 Hz, 1H), 6.17 J=0.82 Hz, 1H), 6.89-6.97 2H), 7.09-7.13 (m, OH O 1H), 7.21-7.25 1H). Molecular formula Cl6HI605 IR(cm.) (KBr) Calcd. C, 66.66; H, 5.94.

3020, 2980, 2954, 2934, 1734, 1640, 1609, 1553, 1448, 1255, 1033, 998, Found C, 66.44; H, 5.63.

OMe 911 0 0 Mass (EI) 288 Compound 130 NMR (ppm) (300 MHz, CD30D) mp. 69-71 °C 0.98 J=7.42 Hz, 3H), 1.71-1.86 1H), 1.99-2.24 IH), 2.28 Elemental analysis J=0.82 Hz, 3H), 3.83 3H), 5.33 J=7.14 Hz, 1H), 6.15 J=0.82 Hz, OH 0 1H), 6.90-6.98 2H), 7.10-7.14 1H), 7.20-7.27 1H). Molecular formula CI7HI805 IR(cm (KBr) Calcd. C, 67.54; H, 6.00.

2974, 2932, 2874, 1744, 1638, 1603, 1560, 1460, 1241, 998 Found C, 67.61; H, 6.01.

M OMe Mass 302 Compound 131 NMR (ppm) (300 MHz, CD3OD) mp.

1.55 J=6.87 Hz, 3H), 2.31 J=0.82 Hz, 3H), 5.65 J=6.87 Hz, 1H), High resolution mass spectrum OH 0 J=0.82 Hz, IH), 6.93-6.97 (in, 1H), 7.01-7.03 (in, IH), 7.28-7.30 Mlclrfrml 1H24 S IR(cm) (KBr Calcd. 264.0456 1729, 1644, 1609, 1562, 1458, 1236, 998, 913, 702 Found 264.0426 o 0 Mass (El) 264 Compound 13.2 NMR (ppm) (300 MHz, CDCI3) mp. 170-1751C 6.66(s, IH), 7.43-7.58(m, 6H), 7.67-7.71(m, 2H), 7.89-7.93(m, 2H) Elemental analysis Molecular formula Ci8HI204 IR~cm" (KBOCalcd. C, 73.97; H, 4.14 0 0 1742, 1628, 1549 Found C, 73.98; H, 4.18 6 A OMass (CI) 293(M+H)+ Compound 133 NIVR (ppm) (300 MHz, CDCI3) mp. 122-127tC 3.79(s, 2H), 4.41(s, 2H), 5.79(s, IH), 7.23-7.39(m, IOH) Elemental analysis OH 0 Molecular formula C20H604 (KBr) Calcd. 74.9'9; H, 5.03 1729, 1649, 1564, 1454, 967, 731 Found C, 74.83; H, 5.04 0 0 Mass (EI) 320(M-) Compound 134 NMR (ppm) (300 MHz, CDC13) mp. 70-721C 1. 16(t, J=7.14, 0.7H), 1. 17(t, J=7.14, 2.3H), 1.25(t, J=7.41, 3H), 2.54(qd, Elemental analysis OH 0 7.41, 2H), 3.11 J=7.14, 0.45H), 3.12(q, J=7.14, 1.55H), 5.94(t, MlclrfruaCoI0 IR(cm") (KBr) Calcd. C, 61.22; H, 6.16 1738, 1642, 1572, 1396, 828 Found C, 61.22; H: 6.19 JO 0 Mass (El) 196(M') Compound 135 NMR (ppm) (300 MHz, CDCI3) mp. 91-98'C( (0.02 mmHg) 0.99(t, J=7.41,t 3H), 1.00(t, J=7.41, 3H), 1.65-1.76(m, 4H), 2.46(td, J=0.55, High resolution mass spectrum OH 0 7.41, 2H), 3.06(t, J=7.41, 2H), 5.92(d, J=0.55, IH) Molecular formula C12HI604 IR(cm") (neat) Calcd. 224.105 2972, 1717, 1644, 1409, 1562, 1448, 996 Found 224.105 O 0 Mass (El) 224(M-) Compound 136 NMR (ppm) (300 MHz, CDCI3) mp. 40-41C 0.94(t, J=7.41, 6H), 1.35-1.46(m, 4H), 1.59-1.71(m, 4H), 2.49(t, J=~7.41, Elemental analysis 2H), 3.08(t, J=7.41, 2H), 5.92(t, J=0.55, 1H) OH 0 Molecular formula C14H2004 IR(cm") (KBr) Calcd. C, 66.65; H, 7.99 2964, 1725, 1638, 1562, 1452 Found C, 66.53; H, 7.95 O 0 Mass (El) 252(M-) Compound 137 NMR (ppm) (300 MHz, CDCI3) mp. 91r-(0.02mmHg) l.17(d, J=6.86, 0.9H), l.18(d, J=6.86, 2,lH), 1.26(d, J=6.86, 3H), 2.73(m, High resolution mass spectrum OH 0 IH), 3.95(m, lH), 5.92(d, J=0.55, lH) Molecular formula .C12H1604 IR(cm) (neat) Calcd. 224.105 2978, 1736, 1625, 1607, 1560, 1236, 1075, 982 Found 224.104 O 0 Mass (El) 224(M-) Compound 138 NMR (ppm) (300 MHz, CDC13) mp. 61-621C 1.05-1.17(m, 6H), 1.20-1.30(m, 6H), 1.79(m, IH), 3.54(m, IH), 3.95(m, Elemental analysis lH), 5.97(d, J=1.10, lH) OH 0 Molecular formula C12HI204 IR(cm-') (KBr) Calcd. C, 65.45; H, 5.49 1721, 1640, 1547, 1437, 986 Found C, 65.34; H, 5.56 O 0 Mass (El) 220(M-) Compound 139 NMR (ppm) (300 MHz, CDCI3) mp. 103-104'C(0.07 mmHg) 0.98(d, J=6.86, 6H), 0.99(d, J=6.86, 6H), 2.12(m, 1 2.22(m, IlH), 2.3 4(d, High resolution mass spectrum OH 0 ~J=7.14, 2H), 2.96(dd, J=1l-.10, 6.86, 2H), 5.91 I H) MlclrfruaC420 IR(cm-) (neat) Caled. 252.136 1966, 1734, 1638, 1560, 1456, 1002, 936 Found 252138 O 0 Mass (EI) 252(M') Compound 140 NMR (ppm) (300 MHz, CDCI3) mp.

1.471 9H), 1.475(s, 9H), 3.45 2H), 3.94(d,J= 1.l10, 2H), 6.14(s, I1H) High resolution mass spectrum OH 0 0Molecular formnula C 18 H2408 0 IR(cm') (neat) Calcd. 368.147 0 2984, 1734, 1653, 1570, 1456, 1396, 1373, 1336, 1257, 1230, 1145 Found 368.149 Mass (EI) 368(M-) Compound 141 NMR (ppm) (300 MHz, CDCI3) mp. 156-1581C 3(s, I 6.62(dd, J=1.65, 3.57, 2H), 7.22(dd, J=0.55, 3.57, 1IH), 7.64(dd, Elemental analysis OH 0 J=0.82, 1.65, lH), 7.72(dd, J=0.55, 1.65, 1H), 7.98(dd, J=0.82, 3.84, 0.3H), 0 8.05(dd, J=0.55, 3.84, 0.7H) Molecular formula Cl 4H806 IR(cm-') (KBr) Calcd. C, 61.77; H, 2.96 1731, 1649, 1545, 1520, 1456, 1031, 766 Found C, 61.67; H, 2.94 0 Mass (El) 272(M-) Compound 142 NMR (ppm) (300 MHz, CDC3) mp. 152-1540C 6.46(s, IH), 7.14-7.21(m, 2H), 7.63(dd, J=1.10, 4.95, 1H), 7.72-7.78(m, 2H), Elemental analysis OH 0 8.33(dd, J=l.l10, 4.12, 0.25H), 8.39(dd, J=I. 10, 4.12, 0.75H) Molecular formnula C14H804S2 S IR(cmf' (KBr) Calcd. C, 55.25; H, 2.65; S, 21.07 15, 1620, 1578, 1537, 1415, 812, 714 Found C, 55.14; H, 2.72; S, 21.02 Mass (El) 304(M-) Compound 143 NMR (ppm) (300 MHz, CDCI3) mp. 94-951C 1.20-1.52(m, 10H), 1.70-1.98(m, IOH), 2.39(m, IH), 3.68(m, IH), 5.89(s, Elemental analysis Molecular formula Ci8H2404 2930, 1729, 1638, 1553 Found C, 70.84; H, 7.89 0 1,0Mass (El) 304(M') Compound 144: NMR (ppm) (300 MHz, CDC3) mp. 46-481C 1.62-2.05(m, 16H), 2.87(m, 1H), 4.1 1(m, 1H), 5.94(s, 1H) Elemental analysis.

Molecular formula C16H2004 IR(cm") (KBr Calcd. C, 69.55; H, 7.29 1727, 1636, 1603, 1560 Found C, 69.11; H, 7.26 Mass (ED) 276(M-) Compound 145 NMR (ppm) (300 MHz, CD3O1D) mp. 200-201 C .2.18(s, 3H), 2.27(d, 0 3H), 6.07(d, J=0.82, lH) Elemental analysis OH Molecular formula C8HqN04

H

N IRcm")XBOCalcd. C, 52.46; H, 4.95; N, 7.65 O 1684, 1568, 1452,.1400, 1346, 996, 766 Found C, 52.38; H, 4.93;N, 7.63 Mass (El) 183(M-) Compound 146 NMR (ppm) (300 MHz, CD3OD) mp. 209-2160C 2.30(d, J=1.l10, 3H), 6.14(dd, J=0.82, 1.64, 1lH), 7.2 1(dd, J=3.84, 5.22, 1lH), Element al analysis 7.77(dd, J=1.l10, 4.96, 1LH), 7.90(dd, J=l1.l1, 3.84) OH Molecular formnula Ci iH9NO4S

H

N IR(cm-') (KJr) Calcd. C, 52.58; H, 3.61; N, 5.57; S, 12.76 Y O I 1692, 1607, 1593, 1543, 1419, 1311, 733 Found C, 52.48; H, 3.68; N, 5.59; S, 12.67 O 0 Mass (El) 251 Compound 147 NMR (ppm) (300 MHz, CD3OD) mp. 188-l91 I 2.30(d, J=0.82, 3H), 6.13(dd, J=0.82, 1.64, IH), 6.68(dd, J=1.92, 3.57, IH), Elemental analysis 7.29(dd, J=0.82, 3.57, IH), 7.78(dd, J=0.82, 1.92) OH Molecular formnula C ii H9N05

H

N IR(cm-') (KBr) Calcd. C, 56.17; H, 3.86; N, 5.96 0 O 0 Mass (El) 235(M-) Compound 148 NMR (ppm) (300 MHz, CD3OD) mp. 247-248rC 2.25(d, J=l. 10, 3H), 5.94(d, J=1.10, 1H), 7.52(m, lH), 7.92(td, J=1.83, 7.69, Elemental analysis OH 8.19(d, J=7.69, I 8.70(m, IlH), 10.61 (br, I 13.40(s, I H)Moeua rml H IR(cm 1 (KBr) Calcd. C, 58.54; H, 4.09; N, 11.38 N N 1692, 1671, 1582, 1547, 1446 Found C, 58.42; H, 4.19; N, 11.31 0 Mass (El) 246(M') Compound 149 NMR (ppm) (300 MHz, CDC13) p 0* 0.88(3H), 1.2-1.4(m, 22H), 1.70(m, 2H), 2.22(s, 3H), 2.43(t, J=7.58, 2H), Elemental analysis 5.90(s, 1H), 7.89(br, lH), 13.18(s, lH) OH Molecular formula C22H37N04 N (CH 2 14

CH

3 IR(cm') (KBr Caled. C, 69.62; H, 9.83; N, 3.69 T2922, 2852, 1694, 1564 Found C, 69.53; H, 9.82; N, 3.78 O 0 Mass (El) 379(M-) Compound 150 NMR (ppm) (300 MHz, CDCI3) mp. 112-il3t 1.45(m, 2H), 1.69(t, J=7.30, 4H), 2.27(d, J=0.77, 3H), 2.38(t, J=7.30, 2H), Elemental analysis 3.08(t, J=7.30, 2H), 5.94(d, J=0.77, I 16.78(s, I H) OH 0 Molecular formula C13HI606 C02H IR(cm-') (KBr Calcd. C, 58.20; H, 6.01 1748, 1702, 1649, 1605, 1557, 1462, 1259 Found C, 58.14; H, 6.03 JO 0 Mass (El) 268(M') Compound 152 NMR (ppm) (300 MHz, CD30D) mp. 166-168°C 1.12(t, J=7.41, 3H), 2.29(s, 3H), 2.47(q, J=7.41, 2H), 5.44(s, 1H) Elemental analysis OH Molecular formula C8H1003 IR(cm') (KBr) Calcd. C, 62.33; H, 6.54 1678, 1605, 1564, 1491, 1265, 1257 Found C, 62.04; H,6.53 O 0 Mass Compound 153 NMR (ppm) (300 MHz, CD30D) mp. 88.0-89.0 °C 2.36(t, J=0.82 Hz, 3H), 6.29-6.30 1H), 6.41-6.42 1H), 7.59-7.64 Elemental analysis 0 2H), 7.72-7.81 1H), 8.17-8.21 2H).

Molecular formula Cl 3 H 1004 h IR(cm') (KBr) Calcd. C, 67.82; H, 4.38.

1746, 1715, 1638, 1568, 1450, 1249, 1218, 1160, 1050, 1023, 716, 696 Found C, 67.84; H, 4.43.

0 0 Mass (EI) 230 Compound 154 NMR (ppm) (300 MHz, CD30D) mp. 46.5-47.0 °C 1.73-1.91 1H), 2.10-2.27 3H), 2.32 J=0.82 Hz, 3H), 2.33-2.51 Elemental analysis O 2H), 2.84-2.95 1H), 5.68-5.82 2H), 6.24 1H), 6.25 1H).

SMolecular formula C13H1404 O IR(cm-') (KBr) Calcd. C, 66.65; H, 6.02.

2922, 1758, 1723, 1649, 1576, 1444, 1323, 1303, 1288, 1218, 1168, 1122, Found C, 66.59; H, 6.09.

996, 853, 665 O 0 Mass (EI) 234 Compound 155 NMR (ppm) (300 MHz, CD30D) mp. 57.5-58.0 °C 1.00-1.43 5H), 1.67-1.90 6H), 2.32 J=0.82 Hz, 3H), 2.49 Elemental analysis 0 J=6.87 Hz, 2H), 6.09-6.11 1H), 6.22-6.23 IH).

Molecular formula Cl4H1804 O IR(cm-') (KBr) Calcd. C, 67.18; H, 7.25.

2936, 2856, 1777, 1725, 1638, 1572, 1543, 1450, 1216, 1154, 1089, 982 Found C, 67.11; H, 7.30.

S0 0 Mass (EI) 250 Compound 156 NMR (ppm) (300 MHz, CD3OD) mp. 205-211 'C (dec) 2.34 J=0.82 Hz, 3H), 4.49 2H), 6.22 J=0.82 Hz, IH), 7.38 J=8.8 Elemental analysis Hz, 2H), 7.50 J=8.8 Hz, 2H).Moeuafrml 1H4I0 OH Moeua fomlaC4I4IO IR(cm") (KBr) Calcd. C, 56.86; H, 4.77; Cl, 11.99; N, 4.74.

2928, 2586, 1721, 1642, 1560, 1512, 1460, 1000, 835, 789 Found C, 56.23; H, 4.76; 12.34; N, 4.69.

0 0 Mass (FAB) 261 Compound 157 NMR (ppm) (300 MHz, CDCI3) mp. 210-2121C 1.47(s, 18H), 2.27(s, 3H), 5.66(s, IH), 5.94(s, 1H), 7.54(s, 2H), 7.98(d, Elemental analysis OH 0J=15.56, 1H), 8.20(d, J=15.56, IH), 18.31(s, 1H) MlclrfruaC320 KBOCalcd. C, 71.85; H, 7.34 o 0 OH 1717, 1626, 1522, 1433, 1207 Found C, 71.72; H, 7.28 Mass (EL) 384(M-) Compound 158 NMR (ppm) (300 MHz, CDC13) mp. 176-1801C 2.28(d, J=0.81, 3H), 2.52(s, 3H), 5.96(s, 1H), 7.22-7.28(m, 2H), Elemental analysis OH 0 2H), 7.93(d, J=15.71, lH), 8.27(d, J=15.71, IH), 18.04(s, 1H) MoeuafrmlC1H4S IR(cm-') (KBr) Calcd. C, 63.56; H, 4.67; S, 10.60 1705, 1624, 1516, 1493, 1475, 998 Found C, 63.51; H, 4.70; S, 10.53 O 0 SMe Mass (El) 302(M') Compound 159 NMR (ppm) (300 MHz, CDCI3) p 16-5C 1.43(s, 18H), 2.27(d, J=0.70, 3H), 2.89(t, J=7.65, 2H), 3.37(t, J=.5 2H), Elemental analysis OH 0 5.06(s, IH), 5.94(s, 1H), 7.03(s, 2H), 16.77(s, IH) Molecular formnula C23H3005 IR~cm" (KBOCalcd. C, 71.48; H, 7.82 J O 0OH 3648, 2968, 1721, 1651, 1564, 1435, 996 Found C, 71.40; H, 7.74 Mass (El) 386(M-) Compound 160 NMR (ppm) (300 MHz, CD3OD) mp. 95-99 0

C

0.94(t, 3H), 1.25-1.50(m, 24H), 1.72(m, 2H), 2.93(t, J=7.41, 2H), 3.97(m, Elemental analysis 0 2H,48(,J27,I)Molecular formnula C21 H3605 HO (H14H3Icm)Kr Calcd. C, 68.45; H, 9.85 HO a _2920, 2852, 1752, 1665, 1607, 1050 Found C, 68.23; H, 9.82 O 0 Mass (El) 368(M-) Compound' 161 NMR (ppm) (300 MHz, CD3OD) mp. 128.0-129.5 IC 2.34 J=0.82 Hz, 3H), 4.79 2H), 6.24 J=0.82 Hz, 1H). Elemental analysis OH 0 Molecular formula C8H805 OH IR(cm') (KBr) Calcd. C, 52.18; H, 4.38.

3282, 1728, 1642, 1573, 1457, 1240, 936, 993, 969, 936, 839 Found C, 52.11; H, 4.36.

O 0 Mass (El) 184 Compound 162 NMR (ppm) (300 MHz, CD3OD) mp. 122-1251C 2.19(quint, J=6.31, 2H), 2.69(dt, J=1.65, 5.76, 2H), 2.95(t, J=6. 31, 2H), Elemental analysis 5.43 IlH) O OH Molecular formula C9H804 IR(cm-') (KBr) Calcd. C, 60.00; H, 4.48 6 0 01738, 1678, 1566, 1460, 1381, 828, 810 Found C, 60.00; H, 4.49 Mass (El) 1 Compound 163 NMR (ppm) mp.

0 Molecular formula HO IR(cm") (KBr) Calcd.

Mg salt 1736, 1717, 1638, 1562, 1543, 1479, 1033 Found O 0 Mass Compound 164 NMR (ppm) mp.

O Molecular formula HO IR(cm') (KBr) Calcd.

SCa salt 3422, 1719, 1630, 1468, 1365, 1323, 1087, 1067, 1027, 975, 793 Found O 0 Mass Compound 165 NMR (ppm) (300 MHz, CD30D) mp. 128-129 °C 2.37 J=0.82 Hz, 3H), 6.35. J=0.82 Hz, IH), 7.24-7.27 1H), Elemental analysis 7.72-7.73 1H), 8.01-8.03 1H).

OH O Molecular formul'a C12H805S S IR(cm-') (KBr) Calcd. C, 54.54; H, 3.05; S, 12.14.

O 3436, 1744, 1734, 1649, 1632, 1574, 1410, 1232, 992 Found C, 55.00; H, 3.44; S, 12.27.

O

Mass (EI) 264 Compound 166 NMR (ppm) (300 MHz, CD30D) mp. 182-183 'C 2.45 3H), 7.37 J=8.5 Hz, 2H), 7.46-7.57 2H), 7.75 J=8.5 Hz, Elemental analysis 2H), 7.89-7.95 1H), 8.17-8.21 1H).

OH 0 Molecular formula C18HI205 SIR(cm") (KBr) Calcd. C, 70.13; H, 3.92.

1729, 1680, 1613, 1560, 1267, 1228, 1180, 930, 764, 582 Found C, 69.68; H, 4.08.

0O Mass (EI) 308 Compound 167 NMR (ppm) (300 MHz, CD30D) mp. 155-157 °C 7.26-7.29 1H), 7.44-7.53 2H), 7.77-7.79 1H), 7.87-7.93 Elemental analysis 1H), 8.02-8.05 1H), 8.19-8.23 IH).

OH O Molecular formula C 15is H805 S -0.2 S IR(cm (KBr) Calcd. C, 59.28; H, 2.79; S, 10.55.

3082, 1725, 1661, 1613, 1557, 1412, 1253, 1230, 901,758 Found C, 58.99; H, 2.92; S, 10.63.

Mass 300 (M Mass (EI) 300 Compound 168 NMR (ppm) (300 MHz, CD30D) mp. 142-143 °C 2.40 J=0.82 Hz, 3H), 3.76 3H), 6.37 J=0.82 Hz, IH), 7.14-7.19 (br Elemental analysis m, 2H), 7.59-7.72 1H), 7.97-8.00 1H).

OH O Molecular formula Cl5HI2060.2 IR(cm (KBr) Calcd. C, 61.73; H, 4.28.

1727, 1665, 1634, 1578, 1441, 1356, 1294, 1259, 992, 876 Found C, 61.88; H, 4.28.

O OMe Mass (El) 288 Compound 169 NMR (ppm) (300 MHz, CD30D) mp. 182-184 C 2.38 J=0.82 Hz, 3H),'3.93 2H), 6.38 J=0.82 Hz, 1H), 7.09 J=8.8 Elemental analysis SHz, 2H), 7.85 J=8.8 Hz, 2H).

OH O Molecular formula Cl5H1206-0.2 IR(cm') (KBr) Calcd. C, 61.73; H, 4.28.

O 1731, 1640, 1603, 1564, 1431, 1270, 1168, 994, 882 Found C, 61.41; H, 4.32.

0 O Mass (El) 288 Compound 170 NMR (ppm) (300 MHz, CD30D) mp. 124-126 C (dec) 2.34 J=0.82 Hz, 3H), 2.73 3H), 3.06-3.14 IH), 3.26-3.32 1H), Elemental analysis 3.56-3.61 2H), 6.22 J=0.82 Hz, 1H).

OH 0 Molecular formula CIoHI205S SMe IR(cm' l (KBr) Calcd. C, 50.00; H, 5.04; N, 11.66.

1719, 1638, 1618, 1572, 1460, 1421, 1241, 1046, 996, 940 Found C, 49.07; H, 4.93; S, 13.10.

O O Mass (EI) 244 Compound 171 NMR (ppm) (300 MHz, CD30D) mp. 125-128 °C (dec) 2.35 J=0.82 Hz, 3H), 2.83 3H), 3.85 2H), 4.52 J=14.8 Hz, 1H), Elemental analysis 4.74 J=14.8 Hz, 1H), 6.28 J=0.82 Hz, 1H).

OH O O Molecular formula C9H1oO5S SMe IR(cm") (KBr) Calcd. C, 46.95; H, 4.38; S, 13.93.

1711, 1647, 1543, 1456, 1236, 1042, 996, 938, 853 Found C, 46.81; H, 4.34; S, 13.88.

S0 Mass (EI) 230 Compound 172 NMR (ppm) (300 MHz, CDCI3) mp. 105-,107'C 7.23(lH, bs), 4.69, 4,56(2H, 2.95(2H, q, 1.25(3H, O Molecular formula HO' IR(cm-') (KBr) Caled.

2936, 1756, 1655, 1613, 1464, 1276, 1052, 855 Found O 0 Mass Compound 173 NMR (ppm) (300 MHz, CDCI3) mp. 79-809C 4.67, 4.55(2H, 2.73, 2.91(2H, t, 1.75(2H, in), 1.02(3H, t, O Molecular formnula HO Z -IR(cin 4 (KJ~r) Calcd.

2972, 1771, 1752, 1462, 1255, 104, 1011 Found O 0 Mass Compound 174 NMR (ppm) (300 MHz, CDCl3) mp. 80-811C 4.69, 4.55(2H, 3.69(1H, sep, 1.26, 1.24(3H, d, J=6.8) O Molecular formula HO IR(cm') (KBOr Calcd.

2974, 1758, 1661, 1603, 1460, 1261, 1050, 541 Found O 0 Mass Compound 175 NMR (ppm) (300 MHz, CDC13) mp. 120-121lC 4.65, 4.56(2H, 2.98(IH, mn), 1.43(4H, ,m) 0 Molecular formula HO IR(cm') (KBr) Calcd.

1771, 1640, 1603, 1274, 1056, 961 'Found O 0 Mass Compound 176 NMR (ppm) mp.

Molecular formnula HO, CO2Me IR(cm-) Calcd.

Found Mass Compound 177 NMR (ppm) (300 MHz, CD3OD) mp. 239-243 0

C

2.40(s, 3H), 3.50(s, 3H), 5.78(d, J=2.75, I 6.00(m, I1H) OH Molecular formula C7H9N02 IR(cm-') KBr) Calcd. C, 60.42; H, 6.52; N, 10.07 163,8, 1626, 1543, 1537, 1528, 1493, 1328, 1232, 1203 Found jN 0 Me Mass Compound 178 NMR (ppm) (300 MHz, CDCI3) mp. >2401C (sublimation) 2.30(s, 3H), 2.72(s, 3H), 5.82(s, 1H), I 1.26(br, IH), 15.64(s, IH) OH 0 Molecular formula C8H9 N03 IR(cm") (KBr) Calcd. C, 57.48; H, 5.43;.N, 8.38 1678, 1628, 1249 Found JN 0

H

Mass (EL) 167(M-) Compound 179 NMR (ppm) (300 MHz, CDCI3) mp. 158-1601C l.18(t, J=7.32, 3H), l.31(t, J=7.32, 3H), 2.57(q, J=7.32, 2H), 3.l8(q, J7.32, Elemental analysis OH 0 2H), 5.84(s, IH), I l.37(br, 1H, 15.73(s, IH, -OH)Moeuafrml tH3 3 IR(cm-') (KBr) Calcd. C, 61.53; H, 6.71; N, 7.17 1659, 1611, 1450, 1241, 1216 Found C, 61.30; H, 6.68; N, 7.14 N 0

H

Mass (El) 195(M-) Compound 180 NMR (ppm) (300 MHz, CD3OD) mp. 155-l581C 1.98(m, IH), 2.27(m, IH), 2.52(m, 2H), 2.54(s, 3H), 4.88(m, IH) Elemental analysis 0 Molecular formula CgH 1006 HO IR(cm') (KBr) Calcd. C, 50.47; H, 4.71 1752, 1702, 1671, 1611, 1450, 1342, 1172 Found C, 50.42; H, 4.70 H0 2 C 0 0 Mass (Sl) 214(M-) Compound 181 NMR (ppm) (300 MHz, CD3OD) mp. 85.0-85,5 IC 2.44-2.49 2H), 2.99-3.05 (in, 2H), 4.75 2H), 5.00-5.16 (in, 2H), Elemental analysis 5.94-5.98 (in, IH) O Molecular formula C9H 1004 HO IR(cm-') KBr)- Caled. C,.59.33; H, 5.53.

3192, 1775, 1752, 1660, 1615, 1461, 1433, 1244, 1125, 1056, 1016, 953, Found C, 59.28; H, 5.54.

0 0 919,843,756 Mass (El) 182 Compound 182 NMR (ppm) (300 MHz, CD3OD) mp. 115-1161C 2.94(dd, -J=6.04, 17.30, 1IH), 3.09(dd, J=4.39, 17.30, 1IH), 3.69(s, 3H), Elemental analysis 3.72(m, 0.5H), 4.24(m, 0.5H), 5.09(m, lH), 7.25-7.40(m, 0 Molecular formula C15HI406 HO 'N IR(cm') (KBr) Calcd. C, 62.07; H, 4.86 1760, 1734, 1661, 1618, 1448, 1390, 1249, 1238, 1178, 1023, 719 Found C, 61,.82; H, 4.84 MeO 2

C

Mass (El) 290(M-) Compound 183 NMR (ppm) (300 MHz, CD3OD) mp. 100.0-100.5 00 2.32 J=0.82 Hz, 3H), 4.43 2H), 6.19 J=0.82 Hz, 1H), 7.05-7.08 (in, Elemental analysis IH), 7.24-7.27 (mn, LH), 7.35-7.38 (mn, 1H).

0 Molecular formula C12HI004S HO IR(cm-) (KBr) Calcd. C, 57.58; H, 4.02; S, 12.81.

1738, 1711, 1653, 1560, 1460, 998, 938, 859, 770 Found C, 57.57; H, 4.04; S, 1 2.66.

O 0 Mass (El) 250 (M) Compound 184 NMR (ppm) (300 MHz, CD30D) mp. 132.0-135.0 °C 3.48 3H), 4.59 2H), 4.79 2H). Elemental analysis O Molecular formula C7H805 HO OMe IR(cm-) (KBr) Calcd. C,48.84; H, 4.68.

3470, 1763, 1673, 1657, 1607, 1595, 1475, 1437, 1274, 1046, 712 Found C,48.97; H, 4.70.

O 0 Mass (EI) 172 Compound 185 NMR (ppm) (300 MHz, CD30D) mp. 133.5-134.0 IC 2.36 J=0.82 Hz, 3H), 5.35 2H), 6.27 J=0.82 Hz, 1H), 6.95-7.02 Elemental analysis 3H), 7.28-7.34 2H).

OH O Molecular formula C14Hi205 IR(cm- (KBr) Calcd. C, 64.61; H, 4.64.

1725, 1653, 1601, 1566, 1493, 1456, 1427, 1234, 1091, 994, 975, 760, 752, Found C, 64.59; H, 4.63.

0 0 692 Mass (EI) 260 Compound 186 NMR (ppm) (300 MHz, CD30D) mp. 103-105C 2.34 J=0.82 Hz, 3H), 3.80 3H), 4.37 2H), 6.21 J=0.82 Hz, 1H), Elemental analysis 6.90-7.00 2H), 7.11-7.15 1H), 7.26-7.32 1H).

OH 0 Molecular formula CI5HI405 IR(cm (KBr) Calcd. C, 65.69; H, 5.15.

3082, 2922, 2842, 1711, 1651, 1557, 1460, 1330, 1249, 996, 748 Found C, 65.67; H, 5.16.

OMe Mass (EI) 274 Compound 187 NMR (ppm) (300 MHz, CD30D) mp. 130-132 'C 4.91 2H), 6.99-7.06 2H), 7.32-7.49 2H+IH), 7.80-7.87 IH), Elemental analysis 8.11-8.15 1H).

OH Molecular formula Cl5HI004S S iR(cm') (KBr) Calcd. C, 62.92; H, 3.52; S, 11.20.

1727, 1620, 1610, 1555, 1422, 760, 704 Found C, 62.90; H, 3.54; S, 11.38.

2 Mass (EI) 286 Compound 18 8 NMR (ppm) (300 MHz, CD3OD) Trp. 167-169 'C 3.36 3H), 4.85 2H), 7.39-7.5 1 (in, 2H), 7.82-7.88 (in, IH), 8.13-8.17 Elemental analysis OH 0 (,Molecular formula C12HI005 OMe IR(cm") (KBr) Calcd. C, 61.54; H, 4.30.

1717, 1615, 1560, 1431, 1207, 1093, 978, 766, 596 Found C, 61.37; H, 4.36.

0 Mass (El) 234 Compound 189 NMR (ppm) (300 MHz, CD3OD) mp. 203-205 'C 2.39 J=0.82 Hz, 3H), 6.11 2H), 6.31 J=0.82 Hz, lH), 9.19 IH). Elemental analysis OH 0 N \Molecular formula C9HS N404 NN Icm)Kr Calcd. C, 45.76; H, 3.41; N, 23.73.

3164, 2956, 1731, 1638, 1578, 1450, 1421, 1096, 990, 731, 571 Found C, 45.54; H, 3'.52; N, 23.54.

O 0 Mass (El) 236 Compound 190 NMR (ppm) (300 MHz, CD3OD) mp. 118-1201C 2.31l(3H, 2.34(3H, 4.35(2H, 6.17(1H, 7.,12-7.20(m, 4H) OH 0 ~Molecular formnula C 15 H1404 IR~cm") Cal Icd. C, 69.76; H, 5.46 Found Mass (El) Compound 191 NMR (ppm) (300 MHz, CD3OD) MP. 146-147 'C 2.32 J=O.82 Hz, 3H), 4.32 2H), 5.95 2H), 6.19 J=0.82 Hz, IlH), Elemental analysis OH 0 (br s, 1H), 6.78 IH), 6.82 (br s, I IR~cm-) XBOCalcd. C, 57.70; H, 3.55.

3088, 1719, 1649, 1555, 1454, 1325, 1303, 1166, 1106, 994, 853, 770 Found C, 57.50; H, 3.57.

Mass (El) 312 Compound 192 NMR (ppm) (300 MHz, CD30D) mp. 46-48 °C 2.33 J=0.82 Hz, 3H), 2.50 3H), 4.48 2H), 6.23 J=0.82 Hz, 1H), Elemental analysis 7.47 J=8.34 Hz, 2H), 7.84 J=8.34 Hz; 2H).

Molecular formula C15HI406S OMR(cm') (KBr) Calcd. C, 55.89; H, 4.38; S, 9.95.

1782, 1740, 1721, 1650, 1577, 1443, 1282, 1255, 1220, 968 Found C, 55.51; H, 4.41; S, 9.84.

0 0 Mass (EI) .184(M) Compound 193 NMR (ppm) (300 MHz, CD30D) mp. 82-83 "C 2.31-2.32 3H), 3.03 3H), 3.10 3H), 6.09-6.10 1H), 6.27-6.29 Elemental analysis 0 1H).

0K NMe 2 Molecular formula C9H II N04 NMe2 IR(cm') (KBr) Calcd. C, 54.82; H, 5.62; N, 7.10.

3082, 2930, 1744, 1715, 1651, 1574, 1454, 1375, 1325, 1174, 1133, 847 Found C, 54.89; H, 5.59; N, 7.09.

0 0 Mass (EI) 197 Compound 194 NMR (ppm) (300 MHz, CD30D) mp. 119-120 C 2.31-2.32 3H), 3.54-3.58 2H), 3.62-3.66 2H), 3.71-3.77 Elemental analysis 0 4H), 6.12-6.13 1H), 6.29-6.31 1H).

SMolecular formula C t O N IR(cm (KBr) Calcd. C, 55.22; H, 5.48; N, 5.86.

S1731, 1642, 1568, 1433, 1394, 1232, 1210, 1172, 1114, 1062, 835 Found C, 55.13; H, 5.46; N, 5.89.

O 0 Mass (EI) 239 Compound 195 NMR (ppm) (300 MHz, CD30D) mp. 93-95 °C 1.46 J=6.32 Hz, 3H), 2.71-2.89 2H), 4.51-4.70 1H+2H), Elemental analysis 6.96-7.02 2H), 7.30-7.33 1H).

OH 0 Molecular formula C12H1204S S IR(cm') (KBr) Calcd. C, 57.13; H, 4.79; S, 12.71.

3110, 2976, 2932, 2902, 1696, 1562, 1444, 1290, 1267, 1069, 1050, 961, Found C, 57.08; H, 4.82;.S, 12.61.

O 0 905,719 Mass (EI) 252 0 Compound 196 NMR (ppm) (300 MHz, CD3OD) mp. 157-159 2.33 J=0.82 Hz, 3H), 6.18 J=0.82 Hz, 1H), 7.17-7.20 (in, lH), Elemental analysis OH 0 (in, IH), 7.68-7.70 (in, IH), 8.14 2H).MoeuafrmlC1H04 IR(cm") (KBr) Calcd. C, 59.53; H, 3.84; S, 12.23.

s 3086, 1718, 1619, 1521, 1348, 1246, 1209, 994, 725 Found C, 59.16; H, 3.85; S, 12.16.

O 0 Mass (El) 262 Compound 197 NMR (ppm) (300 MHz, CD3OD) mp. 224-226 'C 2.34 J=0.82 Hz, 3H), 3.70 1H), 6.20 J=0.82 Hz, 1H), 6.90-6.94 (in, Elemental analysis OH IlH), 7.16-7.20 (in, 2H), 7.28 -7.3 3 (in, IH), 7.91 J= 15.9 Hz, I1H), 8.30 (d, J=15.9 Hz, I Molecular formula C 15 H 205 0.2H2 0 IR~cm- (KBOCalcd. C, 65.31; H, 4.53.

0 0 3280, 1686, 1647, 1584, 1535, 1263, 1243, 998, 861 F ound C, 65.22; H, 4.73.

OH Mass (El) 272 Compound 198 NMR (ppm) (300 MHz, CD3OD) inp. 127-130 -C 2.33 J=0.82 Hz, 3H), 6.18 J=0.82 Hz, lH), 6.66 (dd, J-3.85 Hz, Elemental analysis J'3=1.65 Hz, 1H), 6.97 J=3.85 Hz, IH), 7.77 J=1.65 Hz, IH), 7.78 (d, OH 0 J= 15.7 Hz, I 8.14 J-=15.7 Hz, IlH). Molecular fon-nula C13HI005, IR(cm') (KBr Calcd. C, 63.41; H, 4.12.

\1737, 1625, 1522, 1382, 1271, 1243, 1019, 997, 755 Found C, 63.44; H, 4.12.

JO 0 Mass (El) 246 Compound 199 NMR (ppm) (300 MHz, CD3OD) rnp. 127-128 'C 2.36 J=0.82 Hz, 3H), 6.25 J=0.82 Hz, IH), 7.84 J=2.74 Hz, I Elemental analysis 8.01 J=15.7 Hz, lH), 8.03 J=2.74 Hz, IH), 8.53 J=15.7 Hz, IH).

OH 0 Molecular formula C12H9NO4S IR(c (KB r) Calcd. C, 54.74; H, 3.45; N, 5.32; S, 12.18.

N\ ~iI~1 1721, 1638, 1539, 1477, 1359, 996, 505 Found C, 54.52; H, 3.52; N, 5.31; S, 12.10.

O 0 Mass (El) 263 Compound 200 NMR (ppm) (360 MHz, CD3OD) mp. 138-140 'C 2.24 J=1.37 Hz, 3H), 2.34 J=0.82 Hz, 3H), 6.19 J=0.82 Hz, IH), Elemental analysis OH 0 7.19-7.22 (in, IH), 7 40-7.42 (in, IH), 7.52 J=1.37 Hz, IH), 7.23-7.74 OH 0 m, I Molecular formula C14HI204S S IR(cm'1) (KBr) Calcd. C, 6 0.85; H, 4.38.

1719, 1657, 1537, 1365, 1277, 999, 696, 535 Found C, 60.58; H, 4.56.

O 0 Mass (El) 276 Compound 201 NMR (ppm) (300 MHz, CD3OD) inp. 246-249' 2.32(s, 3H), 3.39(s, 2H), 6.16(s, I 6.85(d, I1H, J=8.24 Hz), 7. 10(dd, I1H, Elemental analysis J= 1.92, 8.24 Hz), 7.22(d, 1 H, J=l1.92 Hz), 7.90(d, I H, J= 15.3 8 Hz), 8.15(d, O H 0 IH, J=15.38) Molecular formnula C!5HI206 MeOH OHI-m" Kr Calcd. C, 60.00; H, 5.03 O H 3466, 3200(br), 1692, 1607, 1514, 1454, 1290, 1247FonC,5.;H,.0 Mass (El) 288(M-) Compound 202 NMR (ppm) (300 MHz, CD3OD) mp. 170-17]1C 2.32(d, 3H, J=0.82 Hz), 2.83(t, 2H, J=7.96 Hz), 3.3 1l(t, 2H, J=7.96), 3.38(s, Elemental analysis 2H), 6.18(d, 1H,J=0.82 Hz), 6.58(m, 1H), 6.68-6.72(m, 2H) OH 0 Molecular formula C 15 H1406 .H2 0 OH IRcm") K~r)Calcd. C, 58.44; H, 5.23 3520, 3200(br), 1686, 1649, 1551, 1439, 1245, 998 Found C, 58.78; H, 5.39 'O (0 OH Mass (El) 290(M-) Compound 203 NMR (ppm) (300 MHz, CD3OD) mp. 247-250 00C (dec) 2.32 J=0.82 Hz, 3H), 4.32 2H), 5.95 2H), 6.19 J=0.82 Hz, IH), Elemental analysis OH 0 (br s, 1H), 6.78 IH), 6.82 (br s, I H).Moeua n-l IR(cin') (KBr) Calcd. C, 59.80; H, 3.68; N, 4.65.

1719, 1640, 1541, 1344, 1232, Found C, 59.53; H, 3.70; N, 4.61.

0O 0 N0 2 Mass (El) 301 Compound 204 NMR (ppm) (300 MHz, CD3OD) mp. 190-192 'C (dee) 2.32 J=0.82 Hz, 3H), 4.32 2H), 5.95 2H), 6.19 J=0.82 Hz, lH), Elemetlaayi 6.77 (br s, IH), 6.78 1H), 6.82 (br s, IlH).

OH 0 Molecular formula Ci 5Hi 6C1N04 0.2H2 0 'N (KBr) Calcd. C, 57.49; H, 5.28; Cl, 11.31; N,.4.47.

I 3522, 2846, 2584, 1719, 1642, 1562, 1514, 996 Found C, 57.38; H, 5.3 1; Cl, 11.62; N, 4.50.

O 0 NH 3

CI

Mass (FAB) 275 Compound 205 NMR (ppm) (300 MHz, CD3OD) mp.

5.53 J=1.9 Hz, IH), 6.75 J=1.9 Hz, 1H), 7.53-7.56 (in, 3H), 7.89-7.94 OH (mn, 2H).

Molecular formula IR(cin) Calcd.

0. 0 Found Mass 188 Compound 206 NMR (ppm) (300 MHz, CDC13) inp. 89-91 'C 2.32 J=0.82 Hz, 3H), 2.51 3H), 3.95 3H), 6.09 J=0.82 Hz, lH). Elemental analysis OMeO0 Molecular formula C9H1004 (KBr) Calcd. C, 59.33; H, 5.53.

3106, 3010, 2964, 2928, 1714, 1666, 1497, 1474, 1344, 1236, 1224, 1015, Found C, 59.30; H, 5,54.

O 0 966, 769 Mass (El) 182 Compound 207 NMR (ppm) (300 MHz, CD3OD) mp. 224-2271C 0 OH 2.18(s, 3H), 5.48(s, I 7.5-7.6(mn, 2H), 7.70(m, I 7.91-7.95(m, 2H) Elemental analysis Molecular formnula C13HI004 IR(cm-') (KBr) Calcd. C, 67.82; H, 4.38 0 1727, 1671, 1620, 1292 Found C, 67.62; H, 4.47 Mass (El) 230(M-) Compound 208 NMR (ppm) (300 MHz, CD3OD) mp. 91-93 1.41 J=7.14 Hz, IH), 2.32 (,J=0.82 Hz, 3H), 4.44 J=7.41 Hz, 2H), Elemental analysis OH 06.22 J=0.82 Hz, IH). Molecular formula C13HI404 OEt IR(cm') (KBr) Calcd. C, 66.65; H, 6.02.

1744, 1650, 1564, 1424, 1340, 1263, 1099, 998 Found C, 66.77; H, 6.02.

Mass (El) 198 Compound 209 NMR (ppm) (300 MHz, CD3OD) mp. 176-179 'C 2.32 J=0.82 Hz, 3H), 3.96 3H), 6.23 J=0.82 Hz, 1H). Elemental analysis OH 0 Molecular formula C8H805 OWe IR(cm- 1 (KBr) Calcd. C, 52.58; H, 4.39.

1750, 1655, 1564, 1462, 1367, 1338, 1253, 1098, 998 Found C, 52.18; H, 4.38.

O 0 Mass (El) 184 Compound 210 NMR (ppm) (300 MHz, CDCI3) mp. 290-2911C 2.35(s,,3H), 5.96(s, IH), 7.16(t, J=6.59, IH), 7.37(t, J=7.32, IH), 7.62(d, Elemental analysis OH 0 1 1.99(s, 1H), 15.49(s, lH)Moeua o-l C32N0 N O IR(cm') (K.Br) Calcd. C, 63.93; H, 4.95; N, 11.47 H 1644, 1601, 1551, 1491, 1448, 1263, 1249 Found C, 63.82; H, 4.89; N, 11.34 N 0 H Mass (El) 244(M') Compound 211 NMR (ppm) mp.

0 Molecular formula NaO N~IR(cm') Calcd.

-7 ound Z O Mass Compound 212 NMR (ppm) mp.

O Molecular formula NaO IR(cm~') Calcd.

Found O 0 Mass Compound 213 NMR (ppm) mp.

O 0 Molecular formula IR(cm') Calcd.

Found O 0 Mass Compound 214 NMR (ppm) mp.

Molecular formula H IR(cm-') Calcd.

x'A 0Found Mass Compound 215 NMR (ppm) mp.

OH O Molecular formula IR(cm') Calcd.

Found aOH Mass Compound 216 NMR (ppm) mp.

ONa O Molecular formula S IR(cm-) Calcd.

SI Found 0 0 Me Mass Compound 217 NMR (ppm) mp.

ONa O Molecular formula S IR(cm) Calcd.

Found OMe Mass Compound 218 NMR (ppm) mp.

ONa O Molecular formula

(CH

2 14

CH

3 IR(cm') Calcd.

Found O 0 Mass Compound 219 NMR (ppm) mp.

O Molecular formula HO IR(cm') Calcd.

Found O 0 Mass Compound 220 NMR (ppm) mp.

ONa O Molecular formula SC02Me IR(cm-) Calcd.

Found Mass Compound 221 NMR (ppm) mp.

OH 0 Molecular formula SIR(cm-') Calcd.

Found O O Mass Compound 222 NMR.(ppm) mp.

0 Molecular formula HO IR(cm") Calcd.

Found O 0 Mass Compound 223 NMR (ppm) mp.

OH O Molecular formula IR(cm') Calcd.

Found Mass Mass Compound 224 NMR (ppm) mp.

O Molecular formula HO IR(cm-) Calcd.

MeO 2 C Found 0 0 Mass Compound 225 NMR (ppm) mp.

0 O Molecular formula IR(cm) Calcd.

S O 0 Found Mass Compound 226 NMR (ppm) mp.

O Molecular formula HO IR(cm') Calcd.

HO

2 C Found 0 0 Mass Compound 227 NMR (ppm) mnp.

O Molecular formula KO IR(cm-i) Calcd.

Found O 0 Mass Compound 228 NMR (ppm) mp.

0 Molecular formula NaO (CH 2 9 Me IR(cm') Calcd.

Found O 0 Mass Compound 229 NMR (ppm) mp.

OH 0 Molecular formula IR(cm") Calcd.

Found O 0 Mass Compound 230 NMR (ppm) mp.

0 Molecular formula HO (CH 2 5 Me IR(cm") Calcd.

Found O 0 Mass Compound 231 NMR (ppm) (300 MHz, CD30D) mp. 155-158 C 2.34 J=0.82 Hz, 3H), 4.58 2H), 6.22 J=0.82 Hz, 1H), 7.57-7.64 Elemental analysis 1H), 7.70-7.73 1H), 8.15-8.23 2H).

OH O Molecular formula CI4HI NO6 N IR(cm') (KBr) Calcd. C, 58.13; H, 3.83; N, 4.84.

2 3080, 2930, 1709, 1653, 1562, 1526, 1458, 1350, 1238, 996, 938, 857, 731 Found C, 58.06; H, 4.04; N, 5.20.

O 0 Mass (EI) 289 Compound 232 NMR (ppm) (300 MHz, CD30D) mp. 176-182 C (dec) 2.34 J=0.82 Hz, 3H), 4.51 2H), 6.23 J=0.82 Hz, 1H), 7.33-7.36 Elemental analysis 1H), 7.39-7.40 1H), 7.44-7.56 2H).

OH 0 Molecular formula CI4HI4CINO4 IR(cm' (KBr) Calcd. C, 56.86; H, 4.77; N, 4.74.

NH

3 I 3436, 3784, 2578, 1719, 1642, 1562, 1460, 1415, 1238, 998, 781, 770 Found C, 56.66; H, 5.01; N, 4.93.

O O Mass (FAB) 261 Compound 233 NMR (ppm) (300 MHz, CD30D) mp. 186-187 °C 2.33 J=0.82 Hz, 3H), 4.43 2H), 6.20 J=0.82 Hz, 1H), 6.98-7.14 Elemental analysis 3H), 7.30-7.38 1H).

OH 0 Molecular formula CI4H1F04 S IR(cm-') (KBr) Calcd. C, 64.12; H, 4.43.

3090, 1712, 1655, 1558, 1488, 1452, 1318, 1260, 1128, 998, 936, 778 Found C, 63.97; H, 4.43.

0 O0 Mass (EI) 262 Compound 234 NMR (ppm) (300 MHz, CD30D) mp. 181-183 t 2.33 J=0.82 Hz, 3H), 4.42 2H), 6.20 J=0.82, 1H), 7.23-7.27 Elemental analysis 2H), 7.32-7.36 2H).

OH NHSO 2

CF

3 Molecular formula CI5HI2F3N06S SIR(cm-') (KBr) Calcd. C, 46.04; H, 3.09; N, 3.58.

3176, 1698, 1653, 1551, 1412, 1365, 1201, 1147, 1000, 955, 605, 507 Found C, 45.71; H, 3.19; N, 3.49.

O 0 Mass (EI) 391 Compound 235 NMR (ppm) (300 MHz, CD30D) mp. 147-148 "C 2.33 J=0.82 Hz, 3H), 4.43 2H), 6.20 J=0.82, 1H), 7.20-7.24 Elemental analysis 3H), 7.33-7.38 1H).

Molecular formula C s5 HI206F3NS OH O m

SNHSO

2

CF

3 IR(c m (KBr) Calcd. C, 46.04; H, 3.09; N, 3.58.

3232, 1738, 1717, 1640, 1562, 1468, 1421, 1232, 1216, 1197, 1141, 1000, Found C, 45.96; H, 3.39; N, 3.52.

O O 700, 603 Mass (El) 391 Compound 236 NMR (ppm) (300 MHz, CD3OD) m.1011* 2.34 J=0.82 Hz, 3H), 4.68 2H), 6.21 J=0.82, IH), 7.39-7.41 (in, OH 0 S 7.48 (br s, I 7.79-7.83 (in, I 7.91-7.93 (in, I1H).Moeuafrml IR(cm') (KBr Calcd.

1717,.1642, 1555, 1456, 994, 940, 764., 632, 551, 501 Found o 0 Mass (El) 300 Compound 237 NMR (ppm) (300 MHz, CD3OD) mp. 90-92.C 2.35 J=0.82 Hz, 3H), 6.25 J=0.82, 1H), 7.62-7.68 (in, 1H), 7.73-7.78 (in, 1H), 7.83 J=7.42 Hz, 1H), 8.04 J=7.42 Hz, 1H), 8.27-8.37 (in, OH 0 CF 3 2H). Molecular formula IR(cm") (KBr) Calcd.

1721, 1638, 1535, 1489, 1315, 1168, 1125, 1038, 998, 768, 462 Found O 0 Mass (El) 324 Compound 238 NMR (ppm) (300 MHz, CD3OD) mp. 124-125 C.

2.35 J=0.82 Hz, 3H), 6.24 J=0.82, 1H), 7.67-7.73 (mn, 1H), 7.76-7.82 (mn, 1H), 7.98-8.03 (in, 2H), 8.01 J=15.9 Hz, 1H), 8.41 J=15.9 Hz, OH 0 IH). Molecular formula

CF

3 IR(cm 1 (KBr) Calcd.

I ~1727, 1632, 1541, 1441, 1367, 1336, 1176, 1120, 998, 822, 690 Found O0 0 Mass (El) 324 Compound 239 NMR (ppm) (300 MHz, CD3OD) mp. 142-144 IC 2.35 J=0.829 1H),6.23 J=0.82, 1H), 7.78 1=8.24 Hz, 2H), 7.92 (d, J=8.24 Hz, 2H), 7.98 J=15.9 Hz, 1H), 8.42 J=15.9 Hz, 1H).

OH 0 Molecular formula IR(cin') (KBr) Calcd.

0 0 CF 3 1725, 1638, 1528, 1369, 1323, 1170, 1125, 1067, 1013,,996, 849, 833 *Found Mass (ED) 324 230 [EXAMPLE 232] Hematopoietic effect in normal mice Each of the above test compounds was peritoneally administered to C57BL/6 mice (7-week old, male) for three days consecutively at a dose of 10 mg/kg, and the peripheral blood cell count was measured 5 days after the beginning of the administration. Table 1 shows the rate of increase with respect to the control group (100%) which were peritoneally administered 10 mg/kg of physiological saline.

Each of the above test compounds was peritoneally administered to C57BL/6 mice (7-week old, male) for three days consecutively at doses of 1, 10, 33, and 100 mg/kg, and the peripheral blood cell count was measured days after the beginning of the administration. Tables (Table 2:platelets, Table 3:red blood cells, and Table 4:white blood cells) shows the rate of increase with respect to the control group (100%) which were peritoneally administered 10 mg/kg of physiological saline.

231 (Table 1: Blood cell increasing rate Compound Platelet Red blood cell White blood cell 108.8 95.1 105.6 91.8 104.5 110.6 111.1 104.4 88.5 106.3 109.3 94.6 109.0 111.6 113.3 108.8 109.5 118.5 110.2 106.7 104.8 107.6 103.7 105.6 104.1 104.5 101.2 100.2 99.7 102.8 105.0 102.1 104.0 102.9 99.7 99.5 102.7 100.4 100.4 100.2 100.5 100.7 102.7 100.5 100.5 101.0 104.0 105.2 105.6 100.0 102.7 100.6 59.0 121.2 69.3 105 70.2 59.3 55.8 82.0 130.9 51.2 72.0 143.5 48.5 60.0 81.6 65.8 72.6 74.3 55.8 69.4 76.8 68.5 76.9 88.4 73.0 232 103.1 97.8 107.3 103.3 110.7 93.7 99.8 97.7 99.8 97.4 99.6 99.4 109.4 100.4 109.4 107.6 110.6 108.7 98.4 108.1 100.1 111. 1 100.6 96.7 108.3 106.2 101.3 102.3 94.9 103.3 104.1 101. 4 99.0 99 .5 107.0 108.2 105.4 102.4 104.6 102.1 102.0 103.5 103.2 102.4 101 .6 98.5 98.4 104.5 103.9 104.8 105.2 104.4 102.2 102.8 103.3 98.6 101.4 102.8 57.2 80.3 50.4 115 59.5 99.8 80.9 49.1 69.1 61.6 74.9 84.1 62.3 70.2 50.6 57.9 68.8 58.7 95.3 70.7 63.9 70.0 65.2 54.1 51. 47.7 110.9 124.4 96.9 233 86 87 88 96 99 101 105 107 110 ill 112 113 117 129 130 131 132 133 134 137 138 140 143 146 147 153 154 102.8 88.4 95.5 106.3 91.7 96.5 100.7 107 106.1 95.5 95.2 105.6 94.1 108 102.5 100.2 96.5 106.6 105.3 98.2 101.6 111.6 103.0 108.1 113.3 96.9 100.3 112.4 105.5 100 96.5 96. 5 99. 9 102.9 104.9 98.6 98 97.6 95.6 101 .7 97.4 104.1 98.2 102.4 102.5 101 .9 101.4 104.5 104.2 102.4 104.2 102.1 102.2 104.7 101.6 99.8 102 .7 105. 5 111.1 179.6 140 96.2 78.7 80.5 120.9 96.8 115 111.5 112.9 93.7 89.5 142.4 108.2 98.4 127.4 85.3 46..6 41.2 61.6 58.3 41.9 75.2 57.2 112.4 127.7 78.5 62.6 234 155 159 161 162 163 168 171 172 173 176 177 180 182 183 187 188 193 195 196 198 206 208 215 216 218 221 211 212 213 111.9 104 104.6 101. 5 98.3 108.8 108 112.0 98.0 97.0 108.9 112.7 102.2 97.6 98.6 104.9 99.4 89.1 109.6 93.7 89.1 109.8 109.1 97 95.1 115.1 106.0 104.0 110.0 101 .6 102.5 102.7 102.8 102.9 98.5 98 87.0 101 .0 104.0 99.1 99.5 99.6 104.1 102.1 100.5 102 96.5 99. 6 102 96.8 101.4 98.6 100.4 98.2 105.0 97.0 104.0 107.0 56 .1 84.7 53.4 74.6 89.7 86.8 96.5 103.0 126.0 70.0 83.3 67.3 143.2 70.2 85.9 143.4 129.7 158.4 81.7 94 144.4 88.4 93.8 120.3 110.5 63.4 110.0 131.0 94.0 235 214 219 220 222 223 224 225 226 227 100.0 109.2 105.5 102.0 119.0 115.0 113.0 116.0 115.0 103.0 99.6 99.3 100.0 104.0 107.0 100.0 103.0 103.0 93.0 81.9 61.7 129.0 61.0 67.0 74.0 45.0 42.0 (Table 2: Platelet increasing rate Compound Dose (mg/kg) 1 10 33 100 110.7 107.0 99.5 117.3 113.3 108.5 106.7 109.9 107.6 112.6 109.0 99.6 110.0 114.7 104.5 99.7 101.7 111.1 99.3 112.7 106.3 110.7 109.0 111.6 108.8 109.5 109.1 110.2 108.2 109.3 94.8 103.0 112.3 110.9 103.8 98.7 112.7 113.2 107.0 108.4 113.7 103.7 111.5 108.9 111.9 112.3 113.5 107.7 236 172 182 212 214 219 220 222 223 224 225 226 227 228 229 113.6 106.2 110.9 115.4 112.0 101.3 112.0 100.0 109.2 113.3 102.0 119.0 115.0 113.0 116.0 115.0 110.6 102.6 111.0 122.0 112.0 117.5 108.0 110.0 122.0 115.0 107.0 109.0 119.0 97.4 110.0 106.0 120.2 101.7 113.0 124.0 126.0 122.0 115.0 111.0 106.8 105.1 (Table 3: Red blood cell increasing rate Compound Dose (mg/kg) 10.0 33.0 100.0 104.0 102.5 103.3 104.3 103.0 105.1 104.9 105.1 107.5 108.4 100.7 105.0 104.0 102.9 100.2 104.0 105.2 105.6 104.1 107.0 108.2 101.0 98.6 103.0 104.7 103.1 102.8 105.6 105.0 106.1 107.3 105.7 107.8 237 52 183 173 176 213 223 224 226 227 105.2 103.8 105.4 104.1 101.0 104.0 107.0 104.0 107.0 103.0 103.0 103.0 105.0 101.0 108.0 109.0 98.0 101.0 103.6 100.9 102.0 104.0 104.0 102.0 105.0 100.0 103.0 (Table 4: White blood cell increasing rate Compound Dose (mg/kg) 1 10 33 100 1 105.3 129.1 118.2 121.2 99.2 107.3 9 84.6 101.2 148.8 16 126.8 130.9 77.6 23 111.2 143.5 161.4 46 125 115 156.8 172 103.0 115.0 122.0 173 126.0 155.0 158.0 182 120.6 143.2 205.2 212 122.0 111.0 187.0 213 94.0 102.0 125.0 214 93.0 126.0 90.0 219 81.9 93.9 133.2 222 129.0 109.0 109.0 230 104.0 111.5 139.1 238 It was found from Tables 1 to 4 that the test compounds increased platelets, red blood cells, and white blood cells dose-dependently.

Industrial Applicability As mentioned above, the ketone derivatives in the present invention increase platelets, red blood cells, and white blood cells and can preferably be applied to the medical field.

Throughout this specification and the claims which follow, unless the context requires otherwise, the word "comprise", and variations such as "comprises" and "comprising", will be understood to imply the inclusion of a stated integer or step or group of integers or steps but not the exclusion of any other integer or step or group of integers or steps.

S

••g

Claims

1. A method for increasing blood cells, in a patient in need thereof, comprising the administration of an effective dose of a ketone derivative of the following general formula or a pharmacologically acceptable salt thereof: OR 2 A R 1 (i) 0 [In the above general formula R 1 is a hydrogen atom, a halogen atom, a hydrocarbon group having 1 to 19 carbon atoms (in which the hydrocarbon group may have one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a carboxyl group, a carboethoxy group, a carbomethoxy group, and a cyano group), -CO(CH 2 )qQ (wherein q is an integer of from 0 to o and Q is a hydrogen atom, a hydrocarbon group having 1 to 6 carbon atoms (in which the hydrocarbon group may have one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy 240 group, an ethoxy group, a carboxyl group, a carboethoxy group, a carbomethoxy group, a cyano group, a trifluoromethyl group, a methylthio group, a phenylthio group, and a t-butyl group), a heterocyclic ring having 1 to 9 carbon atoms (in which the heterocyclic ring may have one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, and an ethoxy group), a hydroxy group, a thiol group, a thioether group having 1 to 6 carbon atoms, an alkoxy group having 1 to 6 carbon atoms, an acetyl group, an amino group, an acetamido group, a cyano group, a carboxyl ester group (in which the ester portion has 1 to 6 carbon atoms), a carboxyl group, a phosphoric ester group (in which the ester portion has 2 to 6 carbon atoms), a phosphoric group, a sulfonyl group having 1 to 7 carbon atoms, a t-butoxycarbonylamino group, a methylsulfoxide group, a primary amido group, or a secondary amido group), -COCO(CH 2 )rV 3 (wherein r is an integer of 0 or 1 and V 3 is a hydrogen atom, a linear or branched alkyl group having 1 to 6 carbon atoms, an aryl group having 6 to 12 carbon atoms (in which the aryl group may have one or more substituents selected from the group essentially- consisting of a chlorine atom, a bromine atom, a fluorine S- 241 atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a carboxyl group, a carboethoxy group, a carbomethoxy group, and a cyano group), a heterocyclic ring having 1 to 9 carbon atoms (in which the heterocyclic ring may have one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, and an ethoxy group), a hydroxy group, a thiol group, a thioether group having 1 to 6 carbon atoms, an alkoxy group having 1 to 6 carbon atoms, an acetyl group, an amino group, an acetamido group, a cyano group, a carboxyl ester group (in which the ester portion has 1 to 6 carbon atoms), a carboxyl group, a primary amido group, or a secondary amido group), -COCH=CHV 4 (wherein V 4 is an aryl group having 6 to 12 carbon atoms (in which the aryl group may have one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a carboxyl group, a carboethoxy group, a carbomethoxy group, a cyano group, a trifluoromethyl group, a methylthio group, and a phenylthio group) or a heterocyclic ring having 1 to 9 carbon atoms (in which the heterocyclic ring may have one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, 242 a fluorine atom, a hydroxy group, a nitro group, a methoxy group, and an ethoxy group), -CO 2 G (wherein G is a hydrogen atom, a linear or branched alkyl group having 1 to 6 carbon atoms, or an aryl group having 6 to 12 carbon atoms (in which the aryl group may have one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a carboxyl group, a carboethoxy group, a carbomethoxy group, a cyano group, a trifluoromethyl group, a methylthio group, and a phenylthio group), -CONHVj (wherein V, is a hydrogen atom, a linear or branched alkyl group having 1 to 10 carbon atoms, an aryl group having 6 to 12 carbon atoms (in which the aryl group may have one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a carboxyl group, a carboethoxy group, a carbomethoxy group, and a cyano group), or a heterocyclic ring having 1 to 9 carbon atoms (which heterocyclic ring may have one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, and an ethoxy group), 243 -NHCOV 2 (wherein V 2 is a hydrogen atom, a linear or branched alkyl group having 1 to 10 carbon atoms, an aryl group having 6 to 12 carbon atoms (in which the aryl group may have one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a carboxyl group, a carboethoxy group, a carbomethoxy group, and a cyano group), or heterocyclic ring having 1 to 9 carbon atoms (in which the heterocyclic ring may have one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, and an ethoxy group), or -(CH 2 )tJ (wherein t is an integer of from 1 to 10, J is a heterocyclic ring having 1 to 9 carbon atoms (in which the heterocyclic ring may have a substituent selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, and an ethoxy group), a hydroxy group, a thiol group, a thioether group having 1 to 6 carbon atoms, an alkoxy group having 1 to 6 carbon atoms, an acetyl group, an amino group, an acetamido group, a cyano group, a carboxyl ester group (in which the ester portion has 1 to 6 carbon atoms), a 244 carboxyl group, a primary amido group, or a secondary amido group); R 2 is a hydrogen atom, a hydrocarbon group having 1 to 6 carbon atoms, or an acyl group having 2 to 10 carbon atoms; A is represented by the following general formula (II) or (III): (CRH) R6 R4 R 5 (II) (III) wherein n is an integer of 0 or 1, R 3 and R 4 are independently a hydrogen atom, a hydrocarbon group having 1 to 15 carbon atoms (in which the hydrocarbon group may have one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a carboxyl group, a carboethoxy group, a carbomethoxy group, and a cyano group), a 1- hydroxy-1-carboalkoxymethyl group, or a group represented by -(CH 2 )mZ (wherein m is an integer of from 1 to 6, Z is a hydroxy group, a thiol group, a thioether group having 1 to 6 carbon atoms, an alkoxy group having 1 to 6 carbon 245 atoms, an acetyl group, an amino group, an acetamido group, a cyano group, a carboxyl ester group (in which the ester portion has 1 to 6 carbon atoms), a carboxyl group, an aldehyde group, a phosphoric group, a sulfonic group, a phosphoric ester group (in which the ester portion has 1 to 6 carbon atoms), a sulfonic ester group (in which the ester portion has 1 to 6 carbon atoms), a primary amido group, a secondary amido group, an indole group, a monosubstituted phenyl group, a disubstituted phenyl group, or a trisubstituted phenyl group (whose substituent is a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a carboxyl group, a carboethoxy group, a carbomethoxy group, and a cyano group), or R 3 and R 4 may together form -(CH 2 4 or Rs is independently a hydrogen atom, a hydrocarbon group having 1 to 15 carbon atoms (in which the hydrocarbon group may have one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a carboxyl group, a carboethoxy group, a carbomethoxy group, and a cyano group), a 1-hydroxy-1-carboalkoxymethyl group, or a group represented by -(CH 2 1 Y (wherein 1 is an integer of from 1 to 6, Y is a hydroxy group, a thiol group, a S-246-- thioether group having 1 to 6 carbon atoms, an alkoxy group having 1 to 6 carbon atoms, an acetyl group, an amino group, an acetamido group, a cyano group, a carboxyl ester group (in which the ester portion has 1 to 6 carbon atoms), a carboxyl group, an aldehyde group, a phosphoric group, a sulfonic group, a phosphoric ester group (in which the ester portion has 1 to 6 carbon atoms), a sulfonic ester group (in which the ester portion has 1 to 6 carbon atoms), a primary amido group, a secondary amido group, an indole group, a thiophene group, a furan group, a monosubstituted phenyl group, a disubstituted phenyl group, or a trisubstituted phenyl group (whose substituent is a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a carboxyl group, a carboethoxy group, a carbomethoxy group, and a cyano group)); R 6 is independently a hydrogen atom, a halogen atom, a hydrocarbon group having 1 to 6 carbon atoms, or an acyl group having 2 to 19 carbon atoms (in which the acyl group may have one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a carboxyl group, a carboethoxy group, a carbomethoxy group, and a cyano group); or R, and R 6 may together form -CH=CH-CH=CH- or -CO(CH,) 3 and P:\OPER\PD\20436-97.074 15/3/00 -247- X is O, S, CH 2 or NL (wherein L is a hydrogen atom, a linear or branched alkyl group, or L may form CH 2 SC(CH 3 2 or -(CH 2 3 with R 3 or R 4

2. A method according to claim 1 for the treatment and prevention of cytopenia.

3. A method according to claim 1 or 2 wherein in the general formula R 1 is -CO(CH 2 )qQ (wherein q is an integer from 0 to 10 and Q is a hydrogen atom, a hydrocarbon group having 1 to 6 carbon atoms (in which the hydrocarbon group may have one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a carboxyl group, a carboethoxy group, a carbomethoxy group, a cyano group, a trifluoromethyl group, a methylthio group, a phenylthio group, and a t-butyl group), a heterocyclic ring having 1 to 9 carbon p o 248 atoms (in which the heterocyclic ring may have a substituent selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, and an ethoxy group), a hydroxy group, a thiol group, a thioether group having 1 to 6 carbon atoms, an alkoxy group having 1 to 6 carbon atoms, an acetyl group, an amino group, an acetamido group, a cyano group, a carboxyl ester group (in which the ester portion has 1 to 6 carbon atoms), a carboxyl group, a phosphoric ester group (in which the ester portion has 2 to 6 carbon atoms), a phosphoric group, a sulfonyl group having 1 to 7 carbon atoms, a t- butoxycarbonylamino group, a methylsulfoxide group, a primary amido group, or a secondary amido group), or -COCO(CH 2 )rV 3 (wherein r is an integer of 0 or 1 and V 3 is a hydrogen atom, a linear or branched alkyl group having 1 to 6 carbon atoms, an aryl group having 6 to 12 carbon atoms (in which the aryl group may have one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a carboxyl group, a carboethoxy group, a carbomethoxy group, and a cyano group), a heterocyclic ring having 1 to 9 carbon atoms (in which the heterocyclic ring may have one or more substituents selected from the P:\OPER\PDB\20436-97.074 15/3/00 -249- group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, and an ethoxy group), a hydroxy group, a thiol group, a thioether group having 1 to 6 carbon atoms, an alkoxy group having 1 to 6 carbon atoms, an acetyl group, an amino group, an acetamido group, a cyano group, a carboxyl ester group (in which the ester portion has 1 to 6 carbon atoms), a carboxyl group, a primary amido group, or a secondary amido group).

4. A method according to claim 1 or 2, wherein the general formula R 1 is -CO(CH 2 )qQ (wherein q is an integer of from 0 to 10 and Q is a hydrogen atom, a hydrocarbon group having 1 to 6 carbon atoms (in which the hydrocarbon group may have one or more substituents selected form the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a carboxyl group, a carboethoxy group, a carbomethoxy group, a cyano group, a trifluoromethyl group, a methylthio group, a phenylthio group, and a t-butyl group), a heterocyclic ring having 1 to 9 carbon atoms (in which the heterocyclic ring may have one or more substituents selected from the group essentially g *g a a 250 consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, and an ethoxy group), a hydroxy group, a thiol group, a thioether group having 1 to 6 carbon atoms, an alkoxy group having 1 to 6 carbon atoms, an acetyl group, an amino group, an acetamido group, a cyano group, a carboxyl ester group (in which the ester portion has 1 to 6 carbon atoms), a carboxyl group, a phosphoric ester group (in which the ester portion has 2 to 6 carbon atoms), a phosphoric group, a sulfonyl group having 1 to 7 carbon atoms, a t-butoxycarbonylamino group, a methylsulfoxide group, a primary amido group, or a secondary amido group).

5. A method according to claim 1 or 2, wherein the general formula the *9 hydrocarbon groups in R 1 R 3 R 4 and R 5 are independently a linear or branched alkyl group, an alkenyl group, an alkinyl group, a cycloalkyl group, a cycloalkenyl group,. 9 an aryl group which may have one or more substituents, an 9 alkylaryl group (whose aryl group may have one or more S" substituents), an arylalkyl group (whose aryl group may have one or more substituents), an arylalkenyl group (whose aryl group may have one or more substituents), or an alkenylaryl group (whose aryl group may have one or 251 more substituents).

6. A method according to claim 1 or 2, wherein the general formula the heterocyclic rings in J, Q, V 1 V2, and V 3 are independently selected from the group essentially consisting of thiophene, furan, pyrrole, tetrahydrofuran, N-methylpyrrole, indole, imidazole, pyrrolidine, pyridine, benzothiophene, benzofuran, quinoline, isoquinoline, phthalimide, and phthalide groups (wherein the heterocyclic rings may have one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy S: group, a nitro group, a methoxy group, and an ethoxy group). *f*e o* oo 4 252 S A ketone derivative of the following general formula or a pharmacologically acceptable salt thereof OR 2 R^ R (CH 2 )n 1 R4 [In the above general formula R 1 is a hydrocarbon group having 3 to 19 carbon atoms (in which the hydrocarbon group may have one or more substituentst selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a carboxyl group, a carboethoxy group, a carbomethoxy group, O and a cyano group), -CO(CH 2 )qQ (wherein q is an integer of 0, 1, or from 3 to 10 and Q is 1 to 6 carbon P:\OPER\PDB\20436-97.074 15/3/00 -253- a heterocyclic ring having 1 to 9 carbon atoms (in which the heterocyclic ring has one or more substituents selected form the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, and an ethoxy group), a hydroxy group, a thiol group, a thioether group having 1 to 6 carbon atoms, an alkoxy group having 1 to 6 carbon atoms, an acetyl group, an amino group, an acetamido group, a cyano group, a carboxyl ester group (in which the ester portion has 1 to 6 carbon atoms), a carboxyl group, a phosphoric ester group (in which the ester portion has 2 to 6 carbon atoms), a phosphoric group, a sulfonyl group having 1 to 7 carbon atoms, a t- butoxycarbonylamino group, a methylsulfoxide group, a primary amido group, or a secondary amido group), COCO(CH 2 )rV 3 (wherein r is an integer of 0 or 1 and V 3 is a hydrogen atom, a linear or branched alkyl group having 1 to 6 carbon atoms, an aryl group having 6 to 12 carbon atoms (in which the aryl group oo* 0* 0 s 0 S *ooo e 6 °oo 254 may have one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a carboxyl group, a carboethoxy group, a carbomethoxy group, and a cyano group), a heterocyclic ring having 1 to 9 carbon atoms (in which the heterocyclic ring may have one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, and an ethoxy group), a hydroxy group, a thiol group, a thioether group having 1 to 6 carbon atoms, an alkoxy group having 1 to 6 carbon atoms, an acetyl group, an amino group, an acetamido group, a cyano group, a carboxyl ester group (in which the ester portion has 1 to 6 carbon atoms), a carboxyl group, a primary amido group, or a secondary amido group), -COCH=CHV 4 (wherein V 4 is a heterocyclic ring o ooo tot ft o fto ot 255 having 1 to 9 carbon atoms (in which the heterocyclic ring has one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, and an ethoxy group), -C02G (wherein G is an 4 aryl group having 6 to 12 carbon atoms (in which the aryl group may have one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a carboxyl group, a carboethoxy group, a carbomethoxy group, a cyano group, a trifluoromethyl group, a methylthio group, and a phenylthio group), -CONHV (wherein V, is a hydrogen atom, a linear or branched alkyl group having 1 to 10 carbon atoms, an aryl group having 6 to 12 carbon atoms (in which the aryl group may have one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a carboxyl group, a carboethoxy group, a carbomethoxy group, and a cyano group), or a heterocyclic ring having 1 to 9 carbon atoms (in which the heterocyclic ring may have a substituent selected from the group S' essentially consisting of a chlorine atom, a bromine atom, 256 a fluorine atom, a hydroxy group, a nitro group, a methoxy group, and an ethoxy group)), -NHCOV 2 (wherein -V 2 is a hydrogen atom, a linear or branched alkyl group having 1 to 10 carbon atoms, an aryl group having 6 to 12 carbon atoms (in which the aryl group may have one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a carboxyl group, a carboethoxy group, a carbomethoxy group, and a cyano group) or-a heterocyclic ring having 1 to 9 carbon atoms (in which the heterocyclic ring may have one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, and an ethoxy group)), or -(CH 2 )tJ (wherein t is an integer of from 1 to 10, J is a heterocyclic ring having 1 to 9 carbon atoms (in which the heterocyclic ring may have one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, and an ethoxy group), a hydroxy group, a thiol group, a thioether group having 1 to 6 carbon atoms, an alkoxy group having 1 to 6 carbon atoms, an acetyl group, an 257 amino group, an acetamido group, a cyano group, a carboxyl ester group (in which the ester portion has 1 to 6 carbon atoms), a carboxyl group, a primary amido group, or a secondary amido group); R 2 is a hydrogen atom, a hydrocarbon group having 1 to 6 carbon atoms, or an acyl group having 2 to 10 carbon atoms; X is O, S, CH 2 or NL (wherein L forms -CH 2 SC(CH 3 2 or -(CH 2 3 with R 3 or R 4 n is an integer of 0 or 1; and R 3 and R 4 are independently a hydrogen atom, a hydrocarbon group having 1 to 15 carbon atoms (in which the hydrocarbon group may have one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a carboxyl group, a carboethoxy group, a carbomethoxy group, and a cyano group), a 1-hydroxy-1-carboalkoxymethyl group, or a group represented by -(CH 2 )mZ (wherein m is an integer of from 1 to 6, Z is a hydroxy group, a thiol group, a thioether group having 1 to 6 carbon atoms, an alkoxy 41 0 group having 1 to 6 carbon atoms, an acetyl group, an amino group, an acetamido group, a cyano group, a carboxyl ester group (in which the ester portion has 1 to 6 carbon atoms), a carboxyl group, an aldehyde group, a phosphoric 258 group, a sulfonic group, a phosphoric ester group (in which the ester portion has 1 to 6 carbon atoms), a sulfonic ester group (in which the ester portion has 1 to 6 carbon atoms), a primary amido group, a secondary amido group, an indole group, a monosubstituted phenyl group, a disubstituted phenyl group, or a trisubstituted phenyl group (whose substituent is a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a carboxyl group, a carboethoxy group, a carbomethoxy group, and a cyano group), or R 3 and R 4 may together form -(CH 2 4 or -(CH 2 5

8. A ketone derivative of the general formula or a pharmacologically acceptable salt thereof as set forth in claim 7, wherein X is O, S, or CH 2

9. A ketone derivative of the general formula or a S pharmacologically acceptable salt thereof as set forth in claim 7, wherein X is NL (L is defined as above).

10. A ketone derivative of the general formula or a pharmacologically acceptable salt thereof as set forth in claim 7, wherein R. is -CO(CH 2 )qQ (wherein q is an integer of 0, 1, or from 3 to 10 and Q is P:\OPER\PDB\20436-97.074 15/3/00 -259- a heterocyclic ring having 1 to 9 carbon atoms (in which the heterocyclic ring has one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, and an ethoxy group), a hydroxy group, a thiol group, a thioether group having 1 to 6 carbon atoms, an alkoxy group having 1 to 6 carbon atoms, an acetyl group, an amino group, an acetamido group, a cyano group, a carboxyl ester group (in which the ester portion has 1 to 6 carbon atoms), a carboxyl group, a phosphoric ester group (in which the ester portion has 2 to 6 carbon atoms), a phosphoric group, a sulfonyl group having 1 to 7 carbon atoms), a t- butoxycarbonylamino group, a methylsulfoxide group, a primary amido group, or a secondary amido group), COCO(CH 2 )rV 3 (wherein r is an integer of 0 or 1 and V 3 is a hydrogen atom, a linear or branched alkyl group having 1 S Se S i• 260 to 6 carbon atoms, an aryl group having 6 to 12 carbon atoms (in which the aryl group may have one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a carboxyl group, a carboethoxy group, a carbomethoxy group, and a cyano group), a heterocyclic ring having 1 to 9 carbon atoms (in which the heterocyclic ring may have a substituent selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, and an ethoxy group), a hydroxy group, a thiol group, a thioether group having 1 to 6 carbon atoms, an alkoxy group having 1 to 6 carbon atoms, an acetyl group, an amino group, an acetamido group, a cyano group, a carboxyl ester group (in which the ester portion has 1 to 6 carbon atoms), a carboxyl group, a primary amido group, ;42 or a secondary amido group). S

11. A ketone derivative or a pharmacologically acceptable salt thereof as set forth in claim 7, wherein in the general formula the heterocyclic rings in J, Q, V 1 V2, and V 3 are independently selected from the group essentially consisting of thiophene, furan, pyrrole, tetrahydrofuran, N-methylpyrrole, indole, imidazole, tetrahydrofuran, N-methylpyrrole, indole, imidazole, 261 pyrrolidine, pyridine, benzothiophene, benzofuran, quinoline, isoquinoline, phthalimide, and phthalide groups (wherein the heterocyclic rings have one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, and an ethoxy group).

12. A ketone derivative of the following general formula or a pharmacologically acceptable salt thereof: OR 2 R R, Rs 5 X [In the above general formula R, is -CO(CH 2 )qQ o 9 o P:\OPER\PDB\20436-97.074 15/3/00 -262- a heterocyclic ring having 1 to 9 carbon atoms (in which the heterocyclic ring has one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, and an ethoxy group), a hydroxy group, a thiol group, a thioether group having 1 to 6 carbon atoms, an alkoxy group having 1 to 6 carbon atoms, an acetyl group, an amino group, an acetamido group, a cyano group, a carboxyl ester group, a carboxyl group, a phosphoric ester group (in which the ester portion has 2 to 6 carbon atoms), a phosphoric group, a sulfonyl group having 1 to 7 carbon atoms, a t-butoxycarbonylamino group, a methylsulfoxide group, a primary amido group, or a secondary amide group), -COCO(CH 2 ,)V 3 (wherein r is an integer of 0 or 1 and V 3 is a hydrogen atom, a linear or branched alkyl group having 1 to 6 carbon atoms, an aryl group having 6 to 12 carbon atoms (in which the aryl group Smay have one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, Sa fluorine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a carboxyl group, a carboethoxy r ~r r r 263 group, a carbomethoxy group, and a cyano group), a heterocyclic ring having 1 to 9 carbon atoms (in which the heterocyclic ring may have one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, and an ethoxy group), a hydroxy group, a thiol group, a thioether group having 1 to 6 carbon atoms, an alkoxy group having 1 to 6 carbon atoms, an acetyl group, an amino group, a acetamido group, a cyano group, a carboxyl ester group (in which ester portion has 1 to 6 carbon atoms), a carboxyl group, a primary amide group, or a secondary amide group), -COCH=CHV 4 (wherein V 4 is a heterocyclic ring having 1 to 9 carbon atoms (in which the heterocyclic ring has one or more substituents selected from the group essentially consisting of a S" chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, and an ethoxy group), 0 0 264 -CO 2 G (wherein G is a hydrogen atom, a linear or branched alkyl group having 1 to 6 carbon atoms, an aryl group having 6 to 12 carbon atoms (in which the aryl group may have one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a carboxyl group, a carboethoxy group, a carbomethoxy group, a cyano group, a trifluoromethyl group, a methylthio group, and a phenylthio group), -CONHV, (wherein V I is a hydrogen atom, a linear or branched alkyl group having 1 to 10 carbon atoms, an aryl group having 6 to 12 carbon atoms (in which the aryl group may have one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a carboxyl.group, a carboethoxy group, a carbomethoxy group, and a cyano group) or a heterocyclic ring having 1 to 9 carbon atoms (in which the heterocyclic ring may have a substituent selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, and an ethoxy group)), -NHCOV 2 (wherein V 2 is a hydrogen atom, a linear or branched alkyl group having 1 to 10 carbon atoms, an aryl group having 6 to 12 carbon atoms (in which the aryl group 265 may have one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a carboxyl group, a carboethoxy group, a carbomethoxy group, and a cyano group), or a heterocyclic ring having 1 to 9 carbon atoms (in which the heterocyclic ring may have one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, and an ethoxy group)), or R 2 is a hydrogen atom, a hydrocarbon group having 1 to e o 266 6 carbon atoms, or an acyl group having 2 to 10 carbon atoms; X is 0, S, CH 2 or NL (wherein L is a hydrogen atom or a linear or branched alkyl group); R, is independently a hydrogen atom, a hydrocarbon group having 1 to 15 carbon atoms, a 1-hydroxy-l-carboalkoxymethyl group, or a group represented by -(CH 2 (wherein 1 is an integer of from 1 to 6 and Y is a hydroxy group, a thiol group, a thioether group having 1 to 6 carbon atoms, an alkoxy group having 1 to 6 carbon atoms, an acetyl group, an amino group, an acetamido group, a cyano group, a carboxyl ester group (in which the ester portion has 1 to 6 carbon atoms), a carboxyl group, an aldehyde group, a phosphoric group, a sulfonic group, a phosphoric ester group (in which the ester portion has 1 to 6 carbon atoms), a sulfonic ester group (in which the ester portion has 1 to 6 carbon atoms), a primary amide group, a secondary amide group, an indole group, a thiophene group, a furan group, a monosubstituted phenyl group, a disubstituted phenyl group, or a trisubstituted phenyl group (whose substituent is a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a carboxyl group, a carboethoxy group, a carbomethoxy group, and a cyano group); R 6 is independently a hydrogen atom, a halogen atom, a hydrocarbon group having 1 to 6 carbon atoms, or an acyl group having 2 to 267 19 carbon atoms (in which the acyl group may have one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, an ethoxy group, a carboxyl group, a carboethoxy group, a carbomethoxy group, and a cyano group); or R 5 and R 6 may together form -CO(CH 2 3

13. A ketone derivative or a pharmacologically acceptable salt thereof as set forth in claim 12, wherein in the general formula X is O, S, or CH 2

14. A ketone derivative or a pharmacologically acceptable salt thereof as set forth in claim 12, wherein in the general formula X is NL (L is defined as above).

15. A ketone derivative or a pharmacologically acceptable salt thereof as set forth in claim 12, wherein in the general formula the heterocyclic rings in J, e. Q, V 1 V 2 and V 3 are independently selected from the group essentially consisting of thiophene, furan, pyrrole, tetrahydrofuran, N-methylpyrrole, indole, imidazole, pyrrolidine, pyridine, benzothiophene, benzofuran, quinoline, isoquinoline, phthalimide, and phthalide groups P:\OPER\PDB\20436-97.074 15/3/00 -268- (wherein the heterocyclic rings has one or more substituents selected from the group essentially consisting of a chlorine atom, a bromine atom, a fluorine atom, a hydroxy group, a nitro group, a methoxy group, and an ethoxy group).

16. Use of a ketone derivative of general formula or a pharmacologically acceptable salt thereof, as defined in claim 1, in the manufacture of a medicament for increasing blood cells.

17. Use of a ketone derivative of general formula or a pharmacologically acceptable salt thereof, as defined in claim 1, in the manufacture of a medicament for the treatment or prevention of cytopenia.

18. Use according to claim 16 or 17 wherein the ketone derivative of general formula is as defined in any one of claims 3 to 6.

19. A composition comprising a compound according to anyone of claims 7 to 15 together with a pharmaceutically acceptable excipient.

20. A method of increasing blood cells or method of treatment, substantially as hereinbefore described. *9

21. A compound according to claim 7, or a composition comprising said compound, substantially as hereinbefore described. P:\OPER\PDB20436-97.074 15/3/00 269

22. A use according to claim 16 substantially as hereinbefore described. DATED this 15th day of March 2000 Toray Industries, Inc. By its Patent Attorneys DAVIES COLLISON CAVE