AU719360B2 - Topical compositions containing a beta-cyclodextrin and an amino polysaccharide - Google Patents

Description

WO 97/17977 PCT/GB96/02768 -1- TOPICAL COMPOSITIONS CONTAINING A BETA-CYCLODEXTRIN AND AN AMINO POLYSACCHARIDE This invention relates to a treatment material applicable to wounds, injuries and a wide variety of diseases of the skin to reduce pain, inflammation and to increase the rate of healing of the affected area of the skin.

The chemistry of the inflammation processes of the skin has revealed the role of the enzyme phospholipase in the hydrolysis of lecithin and the release of free arachidonic acid.

Arachidonic acid is rapidly transformed to the leukotriene via lipoxygenase, thereby producing pain and inflammation.

According to the present invention this train of events can be halted by the topical application of clathrating agents in particular a composition for topical application for the relief of inflammation comprising an inert powder, or a gel, cream or dispersion or the like inert carrier, together with a 3cyclodextrin and an amino saccharide or polysaccharide.

Combinations of amino saccharides or polysaccharides and cyclodextrins according to the invention have been shown to be beneficial in the treatment of skin disorders such as eczema and psoriasis, and have unexpectedly been found to be more effective than either component alone. By incorporating the amino polysaccharide and cyclodextrin with a cross linked acrylic polymer, in accordance with a preferred embodiment, a dry sterile dressing for the treatment wounds or injuries may be produced to which sterile water may be added immediately prior to application.

B-cyclodextrin is a cyclic polymer containing seven D-glucose units and has a doughnut-shaped molecular structure including an interior cavity which receive other molecules and form clathrate compounds therewith. While the efficacy of the WO 97/17977 PCT/GB96/02768 -2invention does not depend on the correctness of the theory, it is believed that the cyclodextrin clathrates the arachidonic acid produced by the wound and thus effectively removes it and 6 so prevents or reduces the pain and inflammation associated with it.

Preferably, the amino saccharides and polysaccharides employed include chitin and products derived from chitin, for example by hydrolysis, to produce n-acetyl glucosamine which can then be de-acylated to give glucosamine or glucosamine hydrochloride.

Such compounds have analgesic or or anti-inflammatory properties and the efficacy thereof has been unexpectedly found to be enhanced when used with cyclodextrin an accordance with the invention.

Further combinations of these ingredients have been employed to demonstrate the broad range of applications in creams, gels and dispersions in the treatment or prevention of a wide range of diseases or injuries.

The following examples will serve or illustrate the novelty and benefits of this invention.

Example I 50.0g of glucosamine hydrochloride 50.0g of dimethyl 3. cyclodextrin made up to 1 litre with sterile water.

The solution was then added to a mixture of 1 kg. coconut oil and 40g of an organo phosphate ester emulsifier maintained at After agitation and cooling this produced a lotion suitable for topical application to the skin to combat inflammation.

Example II of Alcosorb* polymers 10.0g glucosamine hydrochloride and 15.0g P. cyclodextrin iodine clathrate The mixture was mixed thoroughly and placed within a sachet made from bonded synthetic polymers measuring 10 x 15 cms. The sealed sachet was maintained in a dry, sterile condition prior to use.

Example III 40g Alcosorb polymers 10 100g glucosamine hydrochloride 150g dimethyl P. cyclodextrin o The mixture was mixed thoroughly prior to the addition of 1800ml of sterile water, added with constant agitation for a period of five minutes.

*o The resultant gel was then preserved in sterile containers.

Example IV 100g chitin Poly-(1-4) P-D-N-acetylglucosamine P. cyclodextrin Alcosorb The ingredients were admixed and ground to a particle size of 50 microns. The resultant powder was suitable for inclusion with synthetic polymers of incorporation with fabricated polymers.

The preparations illustrated but not limited by Examples 1 to IV were then subjected to a series of tests as demonstrated by the following examples.

"Alcosorb" is a trade mark for a cross-linked acrylate co polymer manufactured by Allied Colloids.

WO 97/17977 PCT/GB96/02768 -4- Example VI The lotion prepared as in Example I was applied to the affected tissue on patients suffering from psoriasis or eczema. All reported relief from pruritis within 10 minutes after application and regeneration of dermal tissue was observed on the fourth day.

Example VI A sachet, produced as in Example II, was immersed in 0.5 litres of sterile water for five minutes and applied to a second degree burn on a patients forearm. The dressing was bandaged in place for twenty four hours. Relief from pain occurred within minutes and when the dressing was removed the healing process had commenced.

Example VII Gel, produced as in Example III, was applied to insect bites, minor scalds and burns in a variety of patients.

The response obtained in all cases was the rapid relief from pain and promotion of healing.

Example VIII The powder produced as in Example IV was incorporated in the layer of fabric used to produce sanitary wear. In the case of infants the incidence of infection caused by the micro organism Candida albicans, known as nappy rash, was significantly reduced. Other applications for the reduction of infection by this organism are related to age and sex.

In each of the Examples I to IV the dimethyl 3. cyclodextrin and 1. cyclodextrin may be exchanged without detracting from the principle of the invention.

WO 97/17977 PCT/GB96/02768 Similarly the amino polysaccharides as a group display similar characteristics from a therapeutic standpoint although the chemical and or physical properties may differ.

Example IX A colloidal suspension was prepared from a combination of chitin, chitosan and glucosamine having a particle size of less than 5 microns in an aqueous solution of /-cyclodextrin in the following manner: chitin (poly(l-4)f-N-acetyl-D-glucosamine) chitosan (poly(1-4)-D-glucosamine) glucosamine The above ingredients were introduced with agitation into an aqueous solution containing 0-cyclodextrin and the whole made up to a total volume of 1 litre.

A non-woven polyester fabric was wetted with this suspension by short term immersion (1-2 seconds, nip rollers) then dried prior to forming into 10cm squares for use in surgical dressings as the contact surface with the perturbed tissue.

When applied to lacerated tissue, relief from pain occured within minutes and the healing process was accelerated.

Analysis of the treated fabric showed that the level of the combined ingredients was 1.7% w/w. It is believed that a combination of saccharides and polysaccharides as in this Example is particularly effective.

Claims (5)

1. 2. A composition as claimed in claim 1 in which the amino polysaccharide and cyclodextrin are incorporated with a cross-linked acrylic polymer to form a dry sterile dressing for the treatment of wounds or injuries.
2. 3. A composition as claimed in either of claims 1 or 2 in which the cyclodextrin is P-cyclodextrin or dimethyl P-cyclodextrin.
3. 4. A composition as claimed in any of claims 1 to 3 wherein both a saccharide and a polysaccharide is present. 4 A composition as claimed in any of claims 1 to 4 made into a cream with water and coconut oil by virtue of an organo-phosphate ester emulsifier.
4. 6. A composition as claimed in any of claims 1 to 4 made into a gel with water and a cross-linked acrylate co polymer. .4
5. 7. A composition as claimed in any of claims 1 to 4 in powder form mixed with a cross-linked acrylate co polymer for mixing with water prior to use.