AU715483B2 - Method of preparation of physostigmine carbamate derivatives from eseroline ethers - Google Patents
Method of preparation of physostigmine carbamate derivatives from eseroline ethers Download PDFInfo
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- AU715483B2 AU715483B2 AU38008/97A AU3800897A AU715483B2 AU 715483 B2 AU715483 B2 AU 715483B2 AU 38008/97 A AU38008/97 A AU 38008/97A AU 3800897 A AU3800897 A AU 3800897A AU 715483 B2 AU715483 B2 AU 715483B2
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- C07D487/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00
- C07D487/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00 in which the condensed system contains two hetero rings
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Description
WO 98/27096 PCT/US97/12367 -1- METHOD OF PREPARATION OF PHYSOSTIGMINE CARBAMATE
DERIVATIVES
FROM ESEROLINE
ETHERS
BACKGROUND OF THE INVENTION The present invention relates to a novel process for the preparation of physostigmine carbamate derivatives and to pharmaceutically acceptable salts thereof. The present invention further relates to a novel process for the preparation of eseroline derivatives and to pharmaceutically acceptable salts thereof.
The physostigmine carbamate derivatives encompassed by the compounds of formula below are useful as memory-enhancing and analgesic agents as disclosed in U.S. Pat. No. 4,791,107, issued Dec. 13, 1988; U.S. Pat. No. 5,187,165, issued Feb. 19, 1993; U.S. Pat. No. 5,541,216, issued Jul. 30, 1996; and U.S. Pat.
No. 5,547,977, issued Aug. 20, 1996. The eseroline derivatives encompassed by the compounds of formula (111) are useful as memory-enhancing and analgesic agents as disclosed in U.S. Pat. No. 5,541,216, issued Jul. 30, 1996; Canadian Pat. No.
1,137,489, issued Dec. 14, 1982; and as useful intermediates for making additional memory-enhancing and analgesic agents.
Various methods for the preparation of physostigmine carbamate derivatives are known. See, for example, Hamer et al., U.S. Pat. No. 3,791,107; Brufani et al., U.S. Pat. No. 4,831,155; Wong et al., U.S. Pat. No. 5,302,721; and Wong et al., U.S.
Pat. No. 5,455,354. There remains a need, however, for processes providing higher -2yields, ecologically allowed reagents and/or less costly means for obtaining these compounds.
It would be desirable, therefore to provide novel methods for the economic preparation of physostigmine carbamate derivatives and to eseroline derivatives without the need for ecologically unfavourable halogenated organic solvents.
Throughout the description and claims of the specification the word "comprise" and variations of the word, such as "comprising" and "comprises", is not intended to exclude other additives, components, integers or steps.
SUMMARY OF THE INVENTION In one aspect the present invention provides a process for the preparation of a compound of the formula
R
CH
3 a 0 0 (X)m R R
(I)
wherein R is C 1
-C
6 alkyl; 15 R 1 is hydrogen, C 1
-C
6 alkyl, C3-C 7 cycloalkyl, C 3
-C
7 cycloalkyl-C 1
-C
6 alkyl, 4 C 7 -Cllbicycloalkyl, phenyl, halophenyl or phenyl-C 1
-C
6 alkyl;
R
2 is Ci-C 6 alkyl, C 3
-C
7 cycloalkyl, C 3
-C
7 cycloalkyl-C 1
-C
6 alkyl,
C
7
-C
1 ibicycloalkyl, phenyl, halophenyl or phenyl-C 1
-C
6 alkyl; or R and R 2 taken together with the nitrogen atom to which they are 20 attached form a group of the formula (la) Y (la) wherein Y is hydrogen or C 1
-C
6 alkyl and Z is hydrogen, C 1
-C
6 alkyl, halogen, C 1
-C
6 alkoxy or hydroxy; X is C 1
-C
6 alkyl, C 1
-C
6 alkoxy, halogen or trifluoromethyl; and SA m is 0,1 or 2; CAMy DocumalounonWKN\3a.00doc -3or a pharmaceutically acceptable salt thereof; which process comprises contacting a compound of formula (II)
CH
3 (X)m
H
R R
(II)
wherein R, X and m are as defined above and R 3 is C 1
-C
6 alkyl, with aqueous hydrogen bromide and lithium halide to afford a compound of formula (III) 0 0&
S*
S
H
3 N N
H
R R R• R (111) wherein R, X and m are as defined above; S* contacting the reaction mixture containing the compound of Formula (III) either with an isocyanate of the formula R 1 NCO and isolating a 15 product of formula wherein R 2 is hydrogen; or with a compound of formula (IV) 0 /=N R4 N-C-N R4
(IV)
C.\My Docunmu\flno NNKln800db.oc -4wherein R 4 is hydrogen or Cl-C 6 alkyl to afford a compound of formula (V)
CH
3 9 9, *9*9 46
(V)
wherein R, R 4 X and m are as above; contacting the reaction mixture containing the compound of 5 formula obtained in step with a compound of the formula
R
1
R
2
NH
wherein R 1 and R 2 are as above in the presence of a carboxylic acid of the formula
R
5
COOH
wherein R 5 is C1-C 6 alkyl; and forming and isolating the product of formula 15 In a further aspect the present invention provides a process for the preparation of a product of formula (III) 9.
90 9 0994 9 9 99 *r S 9~~ so 0r
H
3
HO
(X)m N 'N I H R R wherein R is C1-C 6 alkyl; X is C 1
-C
6 alkyl, C 1
-C
6 alkoxy, halogen or trifluoromethyl and m is 0, 1 or 2; or a pharmaceutically acceptable salt thereof; comprising contacting a compound of formula (II) C:\My Doctnenl lonalNKl300h.doc
R
3 0CH 3 (X)m I H I R R (111) wherein R, X and m are as defined above and R 3 is C1-C 6 alkyl, with aqueous hydrogen bromide and lithium halide to afford a compound of formula (III).
BRIEF DESCRIPTION OF THE DRAWINGS Figure 1 shows the effect of lithium bromide (LiBr) in aqueous (aq.) hydrogen bromide (HBr) on the O-dealkylation of eserethole.
10 Figure 2 shows the effect of acid strength on the O-dealkylation of eserethole.
Figure 3 shows the effect of water on the O-dealkylation of eserethole.
15 Figure 4 shows the effect of the relative ratio of lighium bromide/hydrogen bromide on the O-dealkylation of eserethole.
DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Unless otherwise stated or indicated, the term loweralkyl means a straight or branched alkyl group having from 1 to 6 carbon atoms. Examples of alkyl include methyl, ethyl, n-propyl, isobutyl, pentyl, hexyl, and the like.
Unless otherwise stated or indicated, the term lowercycloalkyl means a saturated ring containing 3 to 7 carbon atoms. Examples of lowercycloalkyl include cyclopropyl, cyclohexyl, cycloheptyl, and the like.
Unless otherwise stated or indicated, the term lowerbicycloalkyl means a group having two saturated rings which contain from 7 to 11 carbons and the rings are attached to each other through two carbons.
C:My Dociunctfloa\NKI\38008bdoc WO 98/27096 PCT/US97/12367 -6- Unless otherwise stated or indicated, the term halogen means fluorine, chlorine, bromine or iodine.
Unless otherwise stated or indicated, the term halide means fluoride, chloride, bromide or iodide.* Unless otherwise stated or indicated, the term aryl means an unsubstituted phenyl or aromatic heterocyclic group; or a phenyl or aromatic heterocyclic group substituted with 1, 2 or 3 substituents each of which being independently loweralkyl, loweralkoxy, halogen, hydroxy, trifluoromethyl, phenoxy or benzyloxy.
The term "pharmaceutically acceptable salts" refers to acid addition salts. The expression "pharmaceutically acceptable acid addition salts" is intended to apply to any non-toxic organic or inorganic acid addition salt of the compounds of formula Illustrative inorganic acids which form suitable salts include hydrochloric, hydrobromic, sulphuric, and phosphoric acid and acid metal salts such as sodium monohydrogen orthophosphate, and potassium hydrogen sulfate. Illustrative organic acids which form suitable salts include the mono-, di-, and tricarboxylic acids.
Illustrative of such acids are, for example, acetic, glycolic, lactic, pyruvic, malonic, succinic, glutaric, fumaric, malic, tartaric, citric, ascorbic, maleic, hyroxymaleic, benzoic, hydroxybenzoic, phenylacetic, cinnamic, salicylic, 2 -phenoxybenzoic, and sulfonic acids such as p-toluenesulfonic acid, methanesulfonic acid and 2hydroxyethanesulfonic acid. Such salts can exist in either a hydrated or substantially anhydrous form.
Other methods for preparation of physostigmine carbamate derivatives are known. See, for example, Hamer et al., U.S. Pat. No. 3,791,107; Brufani et al., U.S.
Pat. No. 4,831,155; Wong et al., U.S. Pat. No. 5,302,721; and Wong et al., U.S. Pat.
No. 5,455,354. There remains a need, however, for processes providing higher yields, ecologically allowed reagents and/or less costly means for obtaining these compounds.
-WO 98/27096 PCT/US97/12367 -7- The process of this invention has the following major advantages over the previously known methods: Aqueous hydrobromic acid is used as a dealkylating agent, along with lithium halide, as well as the reaction solvent. This reagent is less expensive than other previously used dealkylating agents such as boron tribromide or aluminum chloride.
No halogenated solvents are employed. Halogenated solvents such as dichloromethane or dichloroethane are environmentally undesirable and are not allowed by government regulations in many countries.
No preparative column chromatography purification is required. Preparative column chromatography is expensive, labor-intensive and limiting in scale-up throughput.
Environmental emission control is more effective as the lithium halide can be recycled.
Dilute acidic medium and mild reaction conditions decrease the risk of erosion.
The compounds of this invention are prepared by utilizing the synthetic steps described below. Throughout the description of the synthetic steps, the substituents "Hal",
"R
2 "R4" and "R 5 shall have the respective meanings given above unless otherwise indicated.
In structural formulae depicting the compounds of this invention, heavy lines coming out of the 3a-carbon and 8a-carbon of the 1,2,3,3a,8,8a-hexahydropyrrolo[2,3-b]indole ring system signify that the two substituents are above the average plane of the three-ring system, whereas dotted lines signify that the two substituents are below the average plane of the three-ring system, and wavy lines signify that the two substituents are both above said plane or below said plane. Because of conformational constraints, the two substituents at the 3a- and 8a- positions must be both above said average plane or both below said average plane. Thus, in formula the substituents at the 3a- and 8a-positions are cis since they are on the same side of the three ring system. Where said substituents are both above the average plane of the three ring system, the configuration will be referred to as 3aS-cis and where both substituents are below the average plane of the ring, the WO 98/27096 PCT/tUS97/12367 -8configuration will be referred to as 3aR-cis. These two types of configurations are depicted below.
Ri
^CH
3 0 a- (X)m I H I R R 3aS-cis Ri R H3 I
CH
3 0 Z N- N (X)m HI R R 3aR-cis Both of said cis isomers, namely, the 3aS-cis isomer and the 3aR-cis isomer are encompassed by each given compound name or structural formula containing wavy lines mentioned above. Furthermore, all mixtures of the 3aS-cis and 3aR-cis isomers including the racemic mixture (1:1 ratio of 3aS-cis:3aR-cis) are encompassed.
SCHEME
.WO 98/27096 PCTIUS97/12367 CH3 R3 N N I H 1I 4 M (X m R
R
conc. HBr Li-Hal step a HO
CH
3 N N (Xm P R R R, NCO step bi (Ill)
N
I-
N
N=
4 0 N
N
I HI (m R R step b2 HR1272 SCHEME (cont.)
N
N 0H 3
R
5 2 H RH 3 0 R 1
R
2 NH IC3 N N stepc T (X)m I H I N R R )r IH R R (X)m R R R R
(I)
In step a, the compound of formula (II) is contacted with aqueous hydrogen bromide and lithium halide at room temperature. The reaction is then heated to a temperature ranging from 80C-1 00C, preferably 90-95 0 C, for a period of time ranging from 1 to 5 hours, preferably 3 to 4 hours. The reaction is then cooled, diluted with water and neutralized with a suitable base, for example, 10% lithium hydroxide or 20% potassium hydroxide. The appropriate compound of formula (III) is then extracted into an organic solvent such as butyl acetate or ethyl acetate and the resulting solution is dried with a drying agent such as potassium carbonate or molecular sieves.
In this application, the term "aqueous hydrogen bromide" is meant to encompass concentrations of hydrogen bromide of from about 20% to about Preferably, the hydrogen bromide concentration is 48% hydrogen bromide, which is commercially available, and more preferably the hydrogen bromide concentration is within the range of from about 25% to about 30%. Lower concentrations of hydrogen bromide can be obtained from 48% hydrogen bromide by dilution with water. In this application, the term "lithium halide" is meant to encompass lithium bromide, lithium chloride and lithium iodide with lithium bromide being preferred.
In step bl, the compound of formula (III) is contacted with either an alkyl isocyanate or a substituted alkyl isocyanate to form a compound of Formula where
R
2 is hydrogen, as represented by structure (Ib) above. In this instance, the reaction temperature is generally between about 0C and about 25 0 C, preferably about 50C to about 10C. The reaction is monitored and the pH is maintained between about 9 AMENDED SHEET WflOQ/i'Tag~ 'SPCT/US97/12367 and 10 by the addition of a base such as, for example, potassium t-butoxide or an acid such as, for example, acetic acid.
In step b2, the compound of Formula (Ill) is contacted with the carbonyldiimidazole -compound of Formula (IV) to provide the imidazole carbamnate product of structure In this instance, the addition is carried out at about 000 to about 2500, preferably about 20 0
C.
In step c, the reaction is typically conducted by adding sequentially a carboxylic acid, such as, for example, acetic acid, and an amine such as tetrahydroisoquinoline to the solution obtained above. The pH of the acidic solution may optionally be acidified to a pH of from about 4.5 to about 6 with an acid, such as acetic acid, prior to contact with the appropriate amine. The addition of the amine is generally carried out from about -1 50C to about 2500, preferably at from about -1 OC to about 201C.
Examples of compounds made by the process of this invention include those listed below as well as the 3aR-cis isomers thereof and mixtures of the 3aS-cis and 3aR-cis isomers. including the racemic mixtures: (3aS-cis)-1 ,2,3,3a,8,8a-hexahydro- 1,3a,8-trimethylpyrrolo[2,3-b]indol-5-ol, (1,2,3,4tetrahydroisoquinolinyl)carbamate; (3aS-cis)- 1,2,3,3a,8,Ba-hexahydro-l ,3a,8-trimethylpyrrolo[2,3-b]indol-5-oI, (1-methyl- 1 ,2,3,4-tetrahydroisoquinolinyl)carbamate; (3aS-cis)-1 ,2,3,3a,8,8a-hexahydro-1 ,3a,8-trimethylpyrrolo[2,3-b]indol-5-ol, (1-ethyl- 1 ,2,3,4-tetrahydroisoquinolinyl)carbamate; (3aS-cis)-1 ,2,3,3a,8,8a-hexahydro-l1,3a,8-trimethylpyrrolo[2,3-b]indol-5-ol, (1 -propyl- 1 ,2,3,4-tetrahydroisoquinolinyl)carbamate; (3aS-cis)-1 ,2,3,3a,8,8a-hexahydro-1 ,3a,8-trimethylpyrrolo[2,3-bindol-5-ol, (1 -butyl- 1 ,2,3,4-tetrahydroisoquinolinyl)carbamate; (3aS-cis)- 1,2,3,3a,8,8a-hexahydro-1 ,3a,8-trimethylpyrrolo[2,3-b]indol-5-ol, (6-chloro- 1 ,2,3,4-tetrahydroisoquinolinyl)carbamate; HRl272 -12- (3aS-cis)-1, ,2,3,3a, 8,8a-hexa hyd ro-1, ,3a, 8-trimethylpyrro lo(2, 3-blindol-5-o, (7-chioro- 1, 2,3,4-tetra hyd ro isoq u inolIi nyl)ca rbamate; (3a3-cis)-1 ,2,3,3a, 8,8a-hexahydro-1, 3a, 8-trimethylpyrrolo[2,3-b]indol-5-ol, (6-chloro- 1 -methyl-i 1, 2 3 ,4-tetra hyd ro isoq uinolIinyl)ca rba mate; (3aS-cis)-1 ,2,3,3a,8,8a-hexahydro-1 ,3a,8-trimethylpyrrolol2,3-b]indol-5-ol, (7-chioro- 1 -methyl-i 2 3 ,4-tetrahydroisoquinolinyl)carbamate; (3aS-cis)-i 3a,8, 8a-hexahydro-1 ,3a,8-trimethylpyrrolo[2, 3-b]indol-5-ol, (6hydroxy-1 ,2,3,4-tetrahydroisoquinolinyl)carbamate; (3aS-cis)-1 ,2,3,3a,8,8a-hexahydro-1 ,3a,8-trimethylpyrrolo[2,3-b]indol-5-ol, (7hydroxy-1 ,2,3,4-tetrahydroisoquinolinyl)carbamate; (3aS-cis)-1,2, 3,3a,8,8a-hexahydro-1 ,3a, 8-trimethylpyrrolo[2, 3-b]indol-5-ol, (6hydroxy-1 -methyl-i ,2,3,4-tetrahydroisoquinolinyl)carbamate;, (3aS-cis)-1 ,2,3,3a,8,8a-hexahydro-1 ,3a,8-trimethylpyrrolo[2,3-b]indol-5-ol, (7hydroxy-1 -methyl-i ,2,3,4-tetrahydroisoquinolinyl)carbamate; (3aS-cis) -1 ,2,3,3a,8,8a-hexahydro-1 ,3a,8-trimethylpyrrolo[2,3-b]indol-5-ol, cyclohexyl carbamate ester; (3aS-cis) 3a,8, 8a-hexahydro-1 ,3a, 8-trimethylpyrrolo[2, 3-b]indol-5-ol, 3chlorophenyl carbamnate ester fumarate; (3aS-cis) -1 ,2,3,3a,8,8a-hexahydro-1 ,3a, 8-trimethylpyrrolo[2 ,3-b]indol-5-ol, 3chlorophenyl carbamnate ester; and (3aS-cis) -1 ,2,3,3a,8,,8a-hexahydro-1 ,3a,8-trimethylpyrrolo[2,3-b]indol-5-ol, 1- (phenyl)ethyl carbamnate ester.
Thus in accordance with the process of this invention, in one of the preferred embodiments, the substituents R and R 3 are both01-06 alkyl and X is hydrogen. In a more preferred form of this embodiment, the substituents R and R 3 are both methyl.
In a most preferred form of this embodiment, the compound of formula (11) is eserethole.
In yet another preferred embodiment, the substituent R is CI-C~ alkyl, X is hydrogen and R, and R 2 together with the nitrogen to which they are attached form A/ AMENCED SHEET HR1272 -12/1- 1,2,3,4-tetrahydroisoquinoline group or 1-methyl-1,2,3,4-tetrahydroisoquinoline group. In a most preferred form of this embodiment the substituent R is methyl.
The following examples are presented in order to illustrate the invention and are not to be construed as limiting the invention in any way.
OFE
SHEET
AMENDED
SHEET
SWO 98/27096 PCT/US97/12367 -13- EXAMPLE 1 Preparation of Eseroline Salicylic Salt Dissolve LiBr (72 g) in water (36 mL) and 48% aq. HBr (40 mL). Cool the clear solution to an ice bath. To this cold solution add eserethole (20 g, 81.22 mmol).
Warm the mixture and heat on an oil bath to 90-100° C for 3-5 h. Cool the mixture to room temperature and pour into ice water (600 mL). Neutralize the acidic solution with LiOH and extract with ethyl acetate (2 X 200 mL). Dry the combined extracts over potassium carbonate (40 g) and filter under nitrogen. The filtrate, containing eseroline (15.97 g, 90% by HPLC) can be used immediately for the preparation of (3aS-cis)-1,2,3,3a,8,8a-hexahydro-1,3a,8-trimethylpyrrolo[2,3-b]indol- (1,2,3,4-tetrahydroisoquinolinyl)carbamate.
Mix the above solution (20 mL) with salicylic acid (0.55 g, 3.98 mmol, 1.1 eq.) in ethyl acetate (5 mL). Concentrate the clear solution to obtain gray crystals which are recrystallized from ethyl acetate to obtain pure eseroline salicylic salt.
Anal. Calcd. for C 20
H
24 0 4
N
2 67.40 C 6.79 H 7.86 N Found: 67.50 C 6.77 H 7.86 N EXAMPLE 2 Preparation of (3aS-cis)-1,2,3,3a,8,8a-hexahydro-1,3a,8-trimethylpyrrolo[2,3-blindol- (1,2,3,4-tetrahvdroisoquinolinvl)carbamate a) Preparation of Eseroline Add LiBr (80 g) to 48% aq HBr (40 mL) and water (40 mL) to make a clear solution.
To this solution add eserethole (21.31 g, 86.63 mmol) at room temperature under nitrogen with stirring. Heat (90-100°C) the brownish clear solution with an oil bath for hours. Cool the dark greenish brown solution to room temperature and pour into ice-water (240 mL). To this solution was add 20% LiOH to pH 9-10. Extract the mixture with ethyl acetate (2 X 150 mL). Wash the combined ethyl acetate solution WO 98/27096 PCTIUS97/12367 -14with brine, dry over potassium carbonate and filter. Concentrate the filtrate (to 100 mL) to provide a residue which contains eseroline (17.76 g, 95% yield, 98.8% purity).
This solution was used immediately in the preparation of the title product.
b) Preparation of (3aS-cis)-1,2,3,3a,8,8a-hexahvdro-1,3a,8-trimethylpyrrolor2,3- (1,2,3,4-tetrahydroisoquinolinyl)carbamate To the solution obtained in Example 2, step add 1,1-carborlyldiimidazole (15.45 g, 95.29 mmol). Stir the reaction solution at room temperature for 15-30 min. To the same reaction mixture add acetic acid (15.59 g, 259.89 mmol, 3 equiv.) and 1,2,3,4tetrahydroisoquinoline (12.69 g, 95.29 mmol, 1.1 equiv). Allow the mixture to stir overnight at ambient temperature under nitrogen. Wash the reddish reaction mixture with water (40 mL). The aqueous solution is then back extracted with ethyl acetate mL). Extract the combined ethyl acetate extracts with dilute hydrochloric acid.
Neutralize the combined acidic extracts with sodium hydroxide to pH 7.0 and extract with cyclohexane (2 x 120 mL). After drying with potassium carbonate, stir the solution with alumina (25 filter and concentrate to yield a residue crystallized from cyclohexane to obtain the title product (22.37 g, 68.42%) as a white granular crystalline solid (99.5% purity by HPLC); m.p. 770C.
Anal. Calcd. for C 23
H
27 0 2
N
3 73.18 C 7.21 H 11.13 N Found: 72.97 C 7.12 H 11.05 N EXAMPLE 3 Preparation of (3aS-cis)-1,2,3,3a,8,8a-hexahydro-1,3a,8-trimethylpyrrolo[2,3-blindol- (1,2,3,4-tetrahydroisoquinolinyl)carbamate Prepare by the method of Example 2 adding sequentially LiBr (36 g) and eserethole (10 g, 40.6 mmol) to a mixture of 18 mL water and 20 mL of aq HBr Heat the solution to 90-100°C and maintain for 5.5 hours.
EXAMPLE 4 WO 98/27096 PCTfUJS97/12367 Preparation of (3aS-cis)-1,2,3,3a,8,8a-hexahydro-1,3a,8-trimethvlpyrrolo[2,3-blindol- (1.2,3,4-tetrahydroisoquinolinyl)carbamate using lithium iodide To a mixture of Lil (3.08 water (2.0 mL) and 2.0 mL of aq HBr add eserethole (0.5 Heat the mixture to 88-95°C for 7 hours. Pour the reaction mixture into ice water (10 mL) and basify with 50% potassium carbonate to pH 9-10.
Extract the mixture with ethyl acetate (2 x 10 mL). Dry the combined extracts and filter. Prepare the title compound using the filtrate, containing eseroline (0.406 g, 91.8% purity) according to the method of Example 2.
EXAMPLE Preparation of (3aS-cis)-1,2,3,3a,8,8a-hexahydro-1,3a,8-trimethylpvrrolo[2,3-blindol- 5-ol, cyclohexyl carbamate ester To a solution of (-)-eseroline (2.2 g, from Example there is added benzene mL) containing cyclohexyl isocyanate (1.2 g) and the mixture is stirred at 25°C for 3 hours. The product is isolated by extraction of the butyl acetate solution with water (200 mL) followed by sodium hydroxide solution (100 mL, 0.5 N) and water (100 mL).
The residue is dried over anhydrous sodium sulfate and the butyl acetate solution is concentrated under reduced pressure to yield the title compound.
EXAMPLE 6 Preparation of (3aS-cis)-1,2,3,3a,8,8a-hexahydro-1,3a,8-trimethylpyrrolo[23-blindol- 3-chlorophenyl carbamate ester fumarate To a solution of (-)-eseroline (2.2 g, from Example there is added 3-chlorophenyl isocyanate (1.5 g) over 1 hour at 5°C and the mixture is stirred at 250C for 3 hours.
The product is isolated as the fumarate salt following water washing, concentration under reduced pressure, chromatographic purification on silica gel and acidification of the purified free base with fumaric acid (1 equiv.).
WO 98/27096 PCTIUS97/12367 -16- EXAMPLE 7 Preparation of (3aS-cis)-1 .2,3,3a,-8,8a-hexahydro- 1 3a,8-trimethvlr~vrrolof2,3-blindol- 5-ol. 3-chlorophenyl-carbamate ester To a solution of (-)-eseroline (2.2 g, from Example there is added 3-chiorophenyl isocyanate (1.6 g) at -5*C over 5 minutes. After stirring for 0.25 hours, the title compound is isolated substantially as described in Example 2.
EXAMPLE 8 Preparation of (3aS-cis)-1 2 ,3,3a,8,8a-hexahvdro- 1,3a,8-trimethvlpvrrolor2,3-blindol- 1 -(Phenvl)ethyl carbamate ester To a solution of (-)-eseroline (2.2 g, from Example there is added methylbenzyl isocyanate (1.5 g) over 1.5 hours at 1000C. The title compound is isolated substantially as described in Example 2.
In many instances, O-dealkylation of eseroline ethers using 48% aqueous hydrogen bromide alone is not a suitable method. For example, the O-dealkylation requires too long a period of time for completion resulting in serious decomposition of the product. Table 1 discloses the O-dealkylation of eserethole and esermethole using 48% aqueous hydrogen bromide.
WO 98/27096 PCT/US97/12367 -17- TABLE 1 O-Dealkylation of Eseroline Ethers Using 48% Aqueous Hydrogen Bromide Alone Compound -48% HBr Reaction Time Remaining Total (hours) Starting Mat.* Impurities Esermethole 10 14.0 3.36 5.8 Eserethole 10 14.0 9.40 35.96 Eserethole 4 14.0 18.21 37.65 *Relative area by HPLC The effects of varying reaction parameters of O-dealkylation of eserethole were studied in such a way that in one set of experiments only one parameter varies while other parameters are kept unchanged. In all of these experiments, 1.00 g of eserethole was heated at 95-100°C with variable amount of LiBr, water and aq. HBr while the overall volume of the reaction solution was maintained at 4.0 mL. The reaction was monitored by HPLC and the reaction time was represented as the time of 90% conversion of eserethole in order to make an easier comparison. It was observed that the reaction rate of the O-dealkylation step increased as the amount of lithium bromide increased (Fig. Table 2 illustrates the data obtained in this Odealkylation reaction.
SWO 98/27096 PCT/US97/12367 -18- TABLE 2 Entry
H
2 0 (mL) LiBr Amount Time (hours)* 1 4 0 g extremely long 2 4 2.0 g 11.0 (23.0 mmol, 5.7 eq.) 3 4 3.0 g (34.5 mmol, 8.5 eq.) 4 4 4.0 g 2.3 (46.0 mmol, 11 eq.) 0 0 *Time for 90% completion with 1.00 g of eserethole, 4.0 mL of HBr at 1000C Similarly, when acid strength was increased, the rate of reaction increased until a proton concentration of about 4.4 M was reached. At that point, the reaction rate leveled off (Fig. In contrast, dilution with water decreased the reaction rate (Fig. The effect of the relative ratio of LiBr/HBr on the deprotection of eserethole is illustrated in Fig. 4.
The relative catalytic effects of various lithium halides as compared to other halides are as follows: LiBr NaBr KBr; Lil LiBr LiCI. Also, NH 4 Br, Et3NHBr, and LiCI showed no catalytic effect at all on O-dealkylation when the other conditions of the reaction were kept unchanged.
Claims (5)
- 2. A process according to claim 1 wherein said lithium halide is lithium bromide. 15 3. A process according to claim 1 wherein said lithium halide is lithium iodide.
- 4. A process according to any one of the preceding claims wherein R and R 3 are C 1 -C 6 alkyl and X is hydrogen. 20 5. A process according to any one of the preceding claims wherein R is methyl and R 3 is methyl.
- 6. A process according to claim 5 wherein the compound of formula (II) is eserethole.
- 7. A process according to claim 1 wherein R is C1-C 6 alkyl, X is hydrogen and R 1 and R 2 together with the nitrogen to which they are attached form 1,2,3,4- tetrahydroisoquinoline group or a 1-methyl-1,2,3,4-tetrahydroisoquinoline group. 0e a a. 4 4. 4
- 44.S S 460 8. A process according to claim 7 wherein R is methyl. /Y' 47, 41- 9. A process according to any one of the preceding claims wherein said aqueous hydrogen bromide is hydrogen bromide with a concentration within the range of from about 20% to about C:\My Documcna\floaNKI aOO8b.doc 22 A process according to any one of the preceding claims 1 wherein said aqueous hydrogen bromide is hydrogen bromide with a concentration of from about 25% to about 11. A process according to claim 1 wherein the compound of formula is selected from the group consisting of (3aS-cis)-1 ,2,3,3a,8,8a-hexahydro-1 ,3a,8-trimethylpyrrolo[2,3-b]indol-5-ol, (1 ,2,3,4-tetrahydroisoquinolinyl)carbamate; (3aS-cis)-1 ,2,3,3a,8,8a-hexahydro-1 ,3a,8-trimethylpyrrolo[2,3-b]indol-5-ol, (1 -methyl-I ,2,3,4-tetrahydroisoquinolinyl)carbamate; (3aS-cis)-1 ,2,3,3a,8,8a-hexahydro-1 ,3a,8-trimethylpyrrolo[2,3-b]indol-5- ol,(1 -ethyl-I 1,2,3,4-tetra hyd roisoq u inoli nyl)carbamate; (3aS-cis)-1 ,2,3,3a,8,8a-hexahydro-1 ,3a,8-trimethylpyrrolo[2,3-b]indol-5- S 01l, (1 -propyl-1 ,2,3,4-tetrahydroisoquinolinyl)carbamate; (3aS-cis)-I ,2,3,3a,8,8a-hexahydro-l ,3a,8-trimethylpyrrolo[2,3-b]indol-5- ol, (1 -butyl-l ,2,3,4-tetrahydroisoquinolinyl)carbamate; (3aS-cis)-l ,2,3,3a,8,8a-hexahydro-I ,3a,8-trimethylpyrrolo[2,3-b]indol-5- ol, (6-chloro-I ,2 ,3,4-tetrahydroisoq uinolinyl)carbamate; (3aS-cis)-l ,2 3a,8, 8a-hexahyd ro-I ,3a, 8-trimethylpyrrolo[2, ol, (7-chloro-1 ,2 ,3,4-tetrahydroisoquinolinyl)carbamate; (3aS-cis)-I ,2 ,3,3a, 8, 8a-hexahyd ro-I ,3a, 8-trimethylpyrrolol2, o1, (6-chloro-I -methyl-l ,2 ,3,4-tetrahydroisoquinolinyl)carbamate; (3aS-cis)-I ,2,3,3a,8,8a-hexahydro-I ,3a,8-trimethylpyrrolo[2,3-b]indol-5- o1, (7-chloro-I -methyl-I ,2 ,3,4-tetrahydroisoquinolinyl)carbamate; ~(3aS-cis)-I ,2 3a,8, 8a-hexahyd ro- 3a ,8-trimethylpyrrolol2, 25 ol, (6-hydroxy-1 ,2,3,4-tetrahydroisoquinoliny)carbamate; (3aS-cis)-l ,2,3,3a,8,8a-hexahydro-l ,3a,8-trimethylpyrrolo[2,3-b]indol-5- ol, (7-hyd roxy-1, ,2,3,4-tetrahyd roisoq uinol inyl)ca rbam ate; (3aS-cis)-I ,2,3,3a,8,8a-hexahydro-l ,3a,8-trimethylpyrrolo[2,3-b]indol-5- o1, (6-hyd roxy-l -methyl-I 3,4-tetrahydroisoq uinolinyl)carbamate; and (3aS-cis)-1 ,2,3,3a,8,8a-hexahydro-I ,3a,8-trimethylpyrrolo[2,3-b]indol- (7-hydroxy-l -methyl- 1,2, 3,4-tetrahyd roisoquinolinyl)carbamate. CAMy Do=lo,,\fNKMNKI8(Xb.do -23- 12. A process according to claim 1 wherein said compound of formula is (3aS-cis)-1,2,3,3a,8,8a-hexahydro-1,3a,8-trimethylpyrrolo[2,3-b]indol-5-ol, (1, 2 ,3,4-tetrahydroisoquinolinyl)carbamate. 13. A process for the preparation of a product of formula (III) H3 (X)m N N I H R R wherein R is C1-C 6 alkyl; X is C 1 -C 6 alkyl, C1-C 6 alkoxy, halogen or trifluoromethyl and m is 0, 1 or 2; or a pharmaceutically acceptable salt thereof; comprising contacting a compound of formula (II) S6 OH 3 CH3 (X)m N N R R (II) 1 io wherein R, X and m are as defined above and R 3 is C1-C 6 alkyl, with aqueous hydrogen bromide and lithium halide to afford a compound of formula (III). a 14. A process according to claim 13 wherein said lithium halide is lithium bromide. A process according to claim 13 wherein said lithium halide is lithium iodide. 16. A process according to any one of claims 13-15 wherein R and R 3 are Ci- C 6 alkyl and X is hydrogen. 17. A process according to any one of claims 13-16 wherein R is methyl and TAS 1 R 3 is methyl. C:\My DocumIcnMs -24- 18. Aprocess according to claim 17 wherein the compound of formula (II) is eserethole. 19. A process according to claim 13 wherein the compound of formula (111) is eseroline. A process according to any one of claims 13-19 wherein said aqueous hydrogen bromide is hydrogen bromide with a concentration within the range of from about 20% to about 21. A process according to any one of claims 13-20 wherein said aqueous hydrogen bromide is hydrogen bromide with a concentration of about 48%. •000 0 22. A compound of the formula 1 as defined in claim 1 when produced by a 15 process according to any one of claims 1 to 0 23. A compound of the formula III as defined in claim 13 when produced by a process according to any one of claims 13-21. CO* 20 24. A process according to claim 1 or claim 13 substantially as hereinbefore described with reference to any of the examples. DATED: 2 September, 1999 PHILLIPS ORMONDE FITZPATRICK Attorneys for: HOECHST MARION ROUSSEL, INC. C: My Do un flo a\NKMI\3N IIb.doc
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
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US08/769409 | 1996-12-19 | ||
US08/769,409 US5677457A (en) | 1996-12-19 | 1996-12-19 | Method of preparation of physostigmine carbamate derivatives from eseroline ethers |
PCT/US1997/012367 WO1998027096A1 (en) | 1996-12-19 | 1997-07-21 | Method of preparation of physostigmine carbamate derivatives from eseroline ethers |
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AU3800897A AU3800897A (en) | 1998-07-15 |
AU715483B2 true AU715483B2 (en) | 2000-02-03 |
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AU38008/97A Ceased AU715483B2 (en) | 1996-12-19 | 1997-07-21 | Method of preparation of physostigmine carbamate derivatives from eseroline ethers |
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US (1) | US5677457A (en) |
EP (1) | EP0946560A1 (en) |
JP (1) | JP2001506259A (en) |
KR (1) | KR20000062263A (en) |
AR (1) | AR009311A1 (en) |
AU (1) | AU715483B2 (en) |
BR (1) | BR9714046A (en) |
CA (1) | CA2273180C (en) |
HU (1) | HUP0000046A3 (en) |
IL (1) | IL130460A0 (en) |
NO (1) | NO993011L (en) |
WO (1) | WO1998027096A1 (en) |
ZA (1) | ZA976713B (en) |
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IL135399A0 (en) * | 1997-10-10 | 2001-05-20 | Chiesi Farma Spa | A process for the preparation of aminocarbonyl derivatives of geneseroline having selective brain anticholinesterase activity |
US6495700B1 (en) | 2002-01-09 | 2002-12-17 | Axonyx, Inc. | Process for producing phenserine and its analog |
CA2543322A1 (en) | 2003-10-21 | 2005-05-12 | Sention, Inc. | Carbamoyl esters that inhibit cholinesterase and release pharmacologically active agents |
MX2009008288A (en) * | 2007-02-02 | 2009-12-01 | Colucid Pharmaceuticals Inc | Compounds that inhibit cholinesterase. |
SE531698C2 (en) | 2007-07-12 | 2009-07-07 | Respiratorius Ab | New bronchodilating a, b unsaturated amides |
EP2182938A2 (en) * | 2007-07-18 | 2010-05-12 | Colucid Pharmaceuticals, Inc. | Methods for promoting wakefulness |
US8593728B2 (en) * | 2009-02-19 | 2013-11-26 | Toyota Motor Engineering & Manufacturing North America, Inc. | Multilayer photonic structures |
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DE2905054C3 (en) * | 1979-02-08 | 1982-04-29 | Schering Ag, 1000 Berlin Und 4619 Bergkamen | Process for the preparation of 4-hydroxyindoles |
IT1199076B (en) * | 1984-03-01 | 1988-12-30 | Consiglio Nazionale Ricerche | PHYSOSTIGMINE DERIVATIVES WITH PROPERTY OF INHIBITION OF ACETICOLINESTERASI AND RELATED PRODUCTION PROCESS |
US4791107A (en) * | 1986-07-16 | 1988-12-13 | Hoechst-Roussel Pharmaceuticals, Inc. | Memory enhancing and analgesic 1,2,3,3A,8,8A-hexahydro-3A,8 (and) 1,3A,8)-di(and tri)methylpyrrolo(2,3-B)indoles, compositions and use |
US5187165A (en) * | 1987-05-15 | 1993-02-16 | Hoechst-Roussel Pharmaceuticals Inc. | Memory enhancing and analgesic 1,2,3a,8,8a-hexahydro-3a,8(and 1,3a,8)-di(and tri)methylpyrrolo[2,3-b]indoles |
US4971992A (en) * | 1989-03-27 | 1990-11-20 | Hoechst-Roussel Pharmaceuticals Inc. | Carbonate derivatives of eseroline |
DK0418770T3 (en) * | 1989-09-19 | 1995-10-30 | Hoechst Roussel Pharma | Process for Chemical Cleavage of 5-Alkoxy Substituted (+ -) - 1,3-Dimethyloxindolylethylamines |
US4914102A (en) * | 1989-09-28 | 1990-04-03 | Hoechst Roussel Pharmaceuticals, Inc. | N-aminocarbamates related to physostigmine, pharmacentical compositions and use |
US4937341A (en) * | 1989-09-28 | 1990-06-26 | Hoechst-Roussel Pharmaceuticals Inc. | Process for preparing N-aminocarbarbamates related to physostigmine |
US5260452A (en) * | 1989-11-03 | 1993-11-09 | Hoechst-Roussel Pharmaceuticals Inc. | 4- and 6-carbamates related to physostigmine and intermediates for the preparation thereof |
US5177101A (en) * | 1989-11-30 | 1993-01-05 | Hoechst-Roussel Pharmaceuticals Incorporated | Memory enhancing and analgesic aminocarbonylcarbamates related to physostigmine |
US5077289A (en) * | 1989-11-30 | 1991-12-31 | Hoechst Roussel Pharmaceuticals Inc. | Memory enhancing and analgesic aminocarbonylcarbamates related to physostigmine |
US5274117A (en) * | 1990-01-22 | 1993-12-28 | Hoechst-Roussel Pharmaceuticals, Inc. | Process for the enantioselective synthesis of intermediates used in the preparation of physostigmine |
US5091541A (en) * | 1990-02-01 | 1992-02-25 | Hoechst-Roussel Pharmaceuticals Inc. | Hexahydropyrrolo(2,3-B)indole carbamates, ureas, amides and related compounds |
US4983616A (en) * | 1990-02-01 | 1991-01-08 | Hoechst-Roussel Pharmaceuticals Inc. | Hexahydropyrrolo(2,3-B)indole carbamates, ureas, amides and related compounds |
US5216017A (en) * | 1990-05-11 | 1993-06-01 | Hoechst-Roussel Pharmaceuticals Incorporated | Pyrrolo[2,3-b]indole-ketones and analogs |
US5173497A (en) * | 1990-05-17 | 1992-12-22 | Hoechst-Roussel Pharmaceuticals Incorporated | Alpha-oxopyrrolo[2,3-B]indole acetic acids, esters, amides and related analogs |
US5264587A (en) * | 1990-05-17 | 1993-11-23 | Hoechst-Roussel Pharmaceuticals Incorporated | Method of preparing α-oxopyrrolo[2,3-b]indole acetic acids and derivatives |
KR0172971B1 (en) * | 1990-06-27 | 1999-02-01 | 문캐로린디 | Tetrehydroisoquinolinylcarbamates of 1,2,3,3a,8,8a-hexahydro-1,3a,8-trimethylpyrrolo(2,3,-6)indole |
US5231093A (en) * | 1991-10-01 | 1993-07-27 | Hoechst-Roussel Pharmaceuticals Incorporated | Carbamate derivatives of 4-amino-3-isoxazolidinones, 3-amino-1-hydroxypyrrolidin-2-ones and 1-amino-1-cyclopropanecarboxylic acid analogs |
US5153193A (en) * | 1991-10-01 | 1992-10-06 | Hoechst-Roussel Pharmaceuticals Incorporated | Carbamate derivatives of 4-amino-3-isoxazolidinones, 3-amino-1-hydroxypyrrolidin-2-ones and 1-amino-1-cyclopropanecarboxylic acid analogs |
US5234941A (en) * | 1991-10-01 | 1993-08-10 | Hoechst-Roussel Pharmaceuticals Incorporated | Carbamate derivatives of 4-amino-3-isoxazolidinones, 3-amino-1-hydroxypyrrolidin-2-ones and 1-amino-1-cyclopropanecarboxylic acid analogs |
FI933264A (en) * | 1992-07-21 | 1994-01-22 | Hoechst Roussel Pharma | FOERFARANDE FOER FRAMSTAELLNING AV FYSOSTIGMINKARBAMATDERIVAT FRAON FYSOSTIGMIN |
US5302721A (en) * | 1992-07-21 | 1994-04-12 | Hoechst-Roussel Pharmaceuticals Incorporated | Method of preparation of physostigmine carbamate derivatives from eseretholes |
US5350762A (en) * | 1993-03-08 | 1994-09-27 | Hoechst-Roussel Pharmaceuticals Incorporated | Tetrahydrocyclopent[B]indole methanamines and related compounds |
-
1996
- 1996-12-19 US US08/769,409 patent/US5677457A/en not_active Expired - Fee Related
-
1997
- 1997-07-21 JP JP52766298A patent/JP2001506259A/en active Pending
- 1997-07-21 WO PCT/US1997/012367 patent/WO1998027096A1/en not_active Application Discontinuation
- 1997-07-21 AU AU38008/97A patent/AU715483B2/en not_active Ceased
- 1997-07-21 HU HU0000046A patent/HUP0000046A3/en unknown
- 1997-07-21 IL IL13046097A patent/IL130460A0/en unknown
- 1997-07-21 BR BR9714046-5A patent/BR9714046A/en not_active IP Right Cessation
- 1997-07-21 CA CA002273180A patent/CA2273180C/en not_active Expired - Fee Related
- 1997-07-21 KR KR1019997005614A patent/KR20000062263A/en not_active Application Discontinuation
- 1997-07-21 EP EP97934961A patent/EP0946560A1/en not_active Withdrawn
- 1997-07-28 ZA ZA9706713A patent/ZA976713B/en unknown
- 1997-08-25 AR ARP970103837A patent/AR009311A1/en unknown
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1999
- 1999-06-18 NO NO993011A patent/NO993011L/en not_active Application Discontinuation
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HUP0000046A2 (en) | 2000-06-28 |
US5677457A (en) | 1997-10-14 |
ZA976713B (en) | 1998-06-19 |
EP0946560A1 (en) | 1999-10-06 |
KR20000062263A (en) | 2000-10-25 |
NO993011L (en) | 1999-08-18 |
BR9714046A (en) | 2000-05-09 |
WO1998027096A1 (en) | 1998-06-25 |
CA2273180C (en) | 2002-10-29 |
IL130460A0 (en) | 2000-06-01 |
JP2001506259A (en) | 2001-05-15 |
CA2273180A1 (en) | 1998-06-25 |
HUP0000046A3 (en) | 2000-09-28 |
AR009311A1 (en) | 2000-04-12 |
NO993011D0 (en) | 1999-06-18 |
AU3800897A (en) | 1998-07-15 |
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