AU707664B2 - Cyclohexapeptidyl bisamine compound, compositions containing said compound and methods of use - Google Patents

Description

Title of the Invention Cyclohexapeptidyl Bisamine Compound, Compositions Containing Said Compound and Methods of Use Background of the Invention The present invention relates to an antifungal and anti-pneumocystis agent of the pneumocandin class, in which the aryl side chain on the 5-aminoethyloxy-4hydroxyornithine subunit of the hexapeptide consists of a naphthoyl group with an OC7H 15 substituent.

EP 0 535 967 generally discloses pneumocandins containing an aminoethyl ether at the C-5 ornithine position as well as an amine group at the 5th position of the hexapeptide, as having antifungal activity. EP 0 535 967 published April 7, 1993 also discloses these pneumocandins.

The compound of the present invention is characterised by an antibiotic spectrum which is largely focused on Candida, Aspergillus, and Pneumocystis carinii. Also, the 15 present compound has safety and pharmacokinetic properties which are superior to other compounds in the class.

*There is an increasing need for agents which are effective against opportunistic mycotic infections by such agents as Candida, Aspergillus, Cryptococcus and Pneumocystis carinii. The present treatments, amphotericin B and fluconazole, cause 20 severe side effects or are only fungistatic. The compound of the present invention is considered to be both safe and fungicidal.

[n:\libaa]01491:tab WO 97/17365 PCT/US96/17715 -2- SUMMARY OF THE INVENTION The present invention relates to a novel cyclohexapeptidyl bisamine compound of the formula:

H

2 N~O

OH

HO 0 NH

NH

0 OH

HO

0 N NH

H

or its acid addition salt.

H

2 N oN of fOCHorm O >o HN OH H H-f-< HO NH O CH 3 0/ HHN HO HO H OH

OH

HO

(I)

or its acid addition salt.

The present invention also relates to a pharmaceutical composition comprised of the compound in combination with a pharmaceutically acceptable carrier.

Additionally, the present invention relates to a method for treating fungal infections, in particular those caused by Candida, Aspergillus and Pneumocystis carinii which comprises administering the compound of formula I to a patient in need of such treatment in an amount effective to treat the fungal infection. The invention additionally relates to a method for preventing Pneumocystis carinii infections in a patient which comprises administration of a preventative amount of the compound of formula I.

WO 97/17365 PCT/US96/17715 -3- Pharmaceutically acceptable salts suitable as acid addition salts as well as salts providing the anion of the quaternary salt are those from acids such as hydrochloric, hydrobromic, phosphoric, sulfuric, maleic, citric, acetic, tartaric, succinic, oxalic, malic, glutamic, pamoic and the like, and include other acids related to the pharmaceutically acceptable salts listed in Journal of Pharmaceutical Science, 66, 2 (1977).

When the compound is a free amine, it is soluble in lower alcohols and polar aprotic solvents such as dimethylformamide (DMF) and pyridine. It is insoluble in solvents such as ether and acetonitrile.

When the compound is a quaternary ammonium salt or protonated amine, it is soluble in water and polar solvents.

The compound of the present invention is useful as an antibiotic, especially as an antifungal agent or as an antiprotozoal agent.

As an antifungal agent, it is useful for the control of both filamentous fungi and yeast. It is especially adaptable to be employed for the treatment of mycotic infections in mammals, especially those caused by Candida species such as C. albicans, C. tropicalis, C. krusei, C. glabrata and C. pseudotropicalis, and Aspergillus species such as A. fumigatus, A.flavus and A. niger. It is also useful for the treatment and/or prevention of Pneumocystis carinii pneumonia to which immune compromised patients are especially susceptible as hereinafter described.

The previously noted solubility properties are advantageous for utilization in therapeutic applications, especially in injectible compositions.

The compound of the present invention may be obtained from natural products or derivatives of natural products through a sequence of reactions seen in the accompanying reaction schemes or from one of the intermediates which are claimed in concurrently filed copending applications.

The compound of the present invention may be prepared by two different methods. In the first method, illustrated in Scheme 1, the starting material which is represented by formula is a natural product disclosed in US Patent No. 5,202,309 issued April 13, 1993.

WO 97/17365 PCT/US96/17715 -4- The 3-hydroxyglutamine residue may be reduced to a 3hydroxyomithine residue (Compound F) using any suitable reducing agent. However, preferred agents include a borane complex such as borane with tetrahydrofuran or dimethylsulfide or BH2Cl with dimethylsulfide or a metal boride such as ZrCl4/NaBH4. Compound F has been described in copending application serial number 07/936,561 filed September 3, 1992. Etherification at the 5-position of the dihydroxyornithine residue of Compound F may be carried out with ethanolamine and a suitable acid catalyst to give bis-amine Compound G. Compound G has been described in copending application serial number 07/936,558 filed September 3, 1992. The dimethylmyristoyl side chain of Compound G may be deacylated enzymatically with Comamonas acidovorans to give triamine Compound H. Compound H is the subject of copending application serial number 08/295,176 filed August 23, 1994. Other suitable enzymatic preparations from a variety of organisms may also be employed for the deacylation. Selective protection of two of the three amine groups of Compound H may be accomplished by any number of reagents. Benzyl 4nitrophenylcarbonate illustrates this protection step to give Compound J and is shown in Scheme 1. Treatment of Compound J with an activated acylating form of 6-n-heptyloxy-2-naphthoic acid gives the reacylated cyclopeptide K. The preferred activated form is the pentafluorophenyl naphthoate ester. The protecting groups are removed by a suitable method, that being hydrogenolysis for CBz, to give the compound of the present invention.

WO 97/17365 PCTIUS96/17715 SCHEME 1 HO OH K) H N O HN ODH H2N HO H

H

0 BHv-M.,S HO NflN 0

OH

OH

HO

H-

2

NCH

2 C~jOH

H

OH

3 benzyl 4-nitrophenylcarbonate

OH

(G)

Carnamonas aciovorans SUBSTITUTE SHEET (RULE 26) WO 97/17365 PCT/US96/17715 -6- CBz CBz O o oOC7H

SOHO

t O O

OOH

N N H H HH N y 0 0 HOH

HNOH

H NH O CH 3 CH3 H2, Pd-C N

H

HO ,OC7H,b HO N

OH

SOH

OH

HO HO (K)

OH

HO 0

OH

OH

HO (I) Alternatively, as shown in Scheme 2, the compound of the present invention may be obtained by subjecting Compound E to enzymatic reaction with a preparation of Comamonas acidovorans or any suitable organism to give the deacylated cyclopeptide Compound L.

Compound L is the subject of US Patent 5,310,873 issued May 10, 1994.

Acylation of the free amine group of Compound L may be accomplished using an activated acylating form of 6-n-heptyloxy-2naphthoic acid. The pentafluorophenyl ester of 6-n-heptyloxy-2naphthoic acid illustrates the transformation of Compound L to Compound M and is preferred. Reduction of Compound M with a suitable reducing agent such as borane-dimethyl sulfide complex affords amine Compound N. Etherification of Compound N with ethanolamine employing an acid catalyst affords the Compound of the present invention.

WO 97/17365 PCT/US96/17715 -7- SCHEME 2 HO OH HO OH 0 HO0 N H N H NH.

o2 HN OH HN0 HN OH HO N 0

CH

3 HO N H H 3 0 N I H N FS 0 HComamonas

N

HO N acidovorans HO -1C O7, 0 OH 0 OH HO HO (L) HO OH HO OH 0 000 HO N H N N. N N H H N H 0 OH OC7H 15

H

2 0N OH OC7His

H

2

N--

HO NH 0= CH, BH 3 -M0 2 S HO NH 0==(CH 3

H

2 NCH2CH 2 0H 0 V H kO OH

OH

HO HO (N)

H

2

OH

0 0 H O A N N HI H

N

H

2 N N H 0 OH OC 7

H

1 HO NH O CH 3 HO

N

OH 0 OH

HO(I

The compounds of the present invention are active against many fungi and particularly against Candida, Aspergillus and Cryptococcus species. The antifungal properties may be illustrated with SUBSTITUTE SHEET (RULE 26) WO 97/17365 PCT/US96/17715 -8the minimum fungicidal concentration (MFC) determination against certain Candida and Cryptococcus organisms in a microbroth dilution assay carried out in a Yeast Nitrogen Base (Difco) medium with 1 percent dextrose (YNBD).

In a representative assay, Compound I was solubilized in 100 percent dimethyl sulfoxide (DMSO) at an initial concentration of mg/ml. Once dissolved, the drug stock was brought to a concentration of 512 gg/ml by dilution in water such that the final DMSO concentration was about 10 percent. The solution was then dispensed via a multichannel pipetter into the first column of a 96-well plate (each well containing 0.075 ml of YNBD), resulting in a drug concentration of 256 gg/ml. Compounds in the first column were diluted 2-fold across the rows yielding final drug concentrations ranging from 256 gg/ml to 0.12 jgg/ml.

Four-hour broth cultures of organisms to be tested were adjusted using a spectrophotometer at 600 nm to equal a 0.5 McFarland Standard. This suspension was diluted 1:100 in YNBD to yield a cell concentration of 1-5 x 104 colony forming units (CFU)/ml. Aliquots of the suspension (0.075 ml) were inoculated into each well of the microtiter plate resulting in a final cell inoculum of 5-25 x 103.

CFU/ml and final drug concentrations ranging from 128 gJg/ml to 0.06 .tg/ml. Each assay includes one row for drug-free control wells and one row for cell-free control wells.

After 24 hours of incubation, the microtiter plates were shaken gently on a shaker to resuspend the cells. The MIC-2000 inoculator was used to transfer a 1.5 microliter sample from each well of the 96-well microtiter plate to a single reservoir inoculum plate containing Sabouraud dextrose agar (SDA). The inoculated SDA plates were incubated for 24 hours at 35 0 C. However, for Cryptoccoccus neoformans strains, SDA plates were inoculated at 48 hours and incubated 48 hours after being spotted on SDA before making minimum fungicidal concentration (MFC) readings. The results were as follows: WO 97/17365 PCT/US96/17715 -9-

MFC

Organism _g/mL C. albicans CLY 539 <0.06 C. albicans MY 1055 <0.06 C. glabrata MY 1381 <0.06 C. guillermondii MY 1019 0.25 C. krusei <0.06 C. parapsilosis MY 1010 <0.06 C. pseudotropicalis MY 2099 <0.06 C. tropicalis MY 1012 <0.06 Cr. neoformans MY 2061 128 Cr. neoformans MY 2062 32 Compound I also shows in vivo effectiveness against fungi.

Growth from an overnight SDA culture of Candida albicans MY 1055 was suspended in sterile saline and the cell concentration determined by hemacytometer count and the cell suspension adjusted to 3.75 x 105 cells/ml. Then 0.2 milliliter of this suspension was administered I.V. in the tail vein of mice so that the final inoculum was 7.5 x 104 cells/mouse.

The assay then was carried out by administering aqueous solutions of Compound I at various concentrations intraperitoneally twice daily for two consecutive days to 18 to 20 gram female DBA/2 mice, which previously had been infected with Candida albicans in the manner described above. Distilled water was administered I.P. to C. albicans challenged mice as controls. After four days, the mice were sacrificed by carbon dioxide gas, paired kidneys were removed aseptically and placed in sterile polyethylene bags containing 5 milliters of sterile saline. The kidneys were homogenized in the bags, serially diluted in sterile saline and aliquots spread on the surface of SDA plates. The plates were incubated at 35 0 C for 48 hours and yeast colonies were enumerated for determination of colony forming units (CFU) per gram of kidneys. Compound I showed greater WO 97/17365 PCT/US96/17715 than 99 percent reduction of recoverable Candida CFUs when dosed at 0.375 and 0.09 mg/kg I.P. as described above.

The compound of the present invention may also be useful for inhibiting or alleviating Pneumocystis carinii infections in immune compromised patients. The efficacy of the compound of the present invention for therapeutic or anti-infective purposes may be demonstrated in studies on immunosuppressed mice.

In a representative study, the effectiveness of Compound I was determined. Mice (weighing approximately 20 grams) were immunosuppressed with dexamethasone in the drinking water mg/L) and maintained on a low protein diet for seven weeks to induce the development of Pneumocystis pneumonia from a latent infection.

Before drug treatment, two mice were sacrificed to confirm the presence of Pneumocystis carinii pneumonia (PCP); both mice were found to have infections. Five mice (weighing approximately 20 grams) were injected twice daily for four days subcutaneously (sc) with the compound in 0.25 ml of vehicle (distilled water). A vehicle control was also carried out. All animals continued to receive dexamethasone in the drinking water and low protein diet during the treatment period. At the completion of the treatment, all animals were sacrificed, the lungs were removed and processed, and the extent of disease determined by microscopic analysis of stained slides. The results of this study showed Compound I was >95 percent effective in reducing P. carinii cysts in mice when dosed at 0.125 mg/kg.

The compound of the present invention has surprising and unexpected safety benefits in that there is markedly improved acute tolerability in mammals than with other related compounds. To demonstrate, laboratory test animals (rhesus monkeys) were dosed with the compound intravenously and observed for any abnormal effects.

Unexpectedly, no notable response was observed for the compound of the present invention when dosed at identical levels of related compounds that do cause cardiovascular effects.

WO 97/17365 PCT/US96/17715 11 Additionally, the acute tolerability of the compound of the present invention in mice has been shown to be superior to that of related compounds.

The outstanding properties are most effectively utilized when Compound I is formulated into novel pharmaceutical compositions with a pharmaceutically acceptable carrier according to conventional pharmaceutical compounding techniques.

The novel compositions contain at least a therapeutic antifungal or antipneumocystis amount of the active compound of the present invention. Generally, the composition contains at least 1 percent by weight of Compound I. Concentrate compositions suitable for dilutions prior to use may contain 90 percent or more by weight. The compositions include compositions suitable for oral, topical, parenteral (including intraperitoneal, subcutaneous, intramuscular, and intravenous), nasal, and suppository administration, or insufflation.

The compositions may be prepacked by intimately mixing Compound I with the components suitable for the medium desired.

Compositions formulated for oral administration may be a liquid composition or a solid composition. For liquid preparations, the therapeutic agent may be formulated with liquid carriers such as water, glycols, oils, alcohols, and the like, and for solid preparations such as capsules and tablets, with solid carriers such as starches, sugars, kaolin, ethyl cellulose, calcium and sodium carbonate, calcium phosphate, kaolin, talc, lactose, generally with a lubricant such as calcium stearate, together with binders disintegrating agents and the like. Because of their ease in administration, tablets and capsules represent the most advantageous oral dosage form. It is especially advantageous to formulate the compositions in unit dosage form (as hereinafter defined) for ease of administration and uniformity of dosage. Compositions in unit dosage form constitute an aspect of the present invention.

Compositions may be formulated for injection and for injection take such forms as suspensions, solutions or emulsions in oily or aqueous vehicles such as 0.85 percent sodium chloride or 5 percent dextrose in water and may contain formulating agents such as WO 97/17365 PCT/US96/17715 -12suspending, stabilizing and/or dispersing agents. Buffering agents as well as additives such as saline or glucose may be added to make the solutions isotonic. Compound I may also be solubilized in alcohol/propylene glycol or polyethylene glycol for drip intravenous administration. These compositions also may be presented in unit dosage form in ampoules or in multidose containers, preferably with added preservative. Alternatively, the active ingredients may be in powder form for reconstituting with a suitable vehicle prior to administration.

The term "unit dosage form" as used in the specification and claims refer to physically discrete units, each unit containing a predetermined quantity of active ingredient calculated to produce the desired therapeutic effect in association with the pharmaceutical carrier.

Examples of such unit dosage forms are tablets, capsules, pills, powder packets, wafers, measured units in ampoules or in multidose containers and the like. A unit dosage of the present invention will generally contain from 10 to 500 milligrams of the compound.

When the compound of the present invention is for antifungal use, any method of administration may be employed.

When Compound I is to be employed for control of pneumocystis infections any method may be employed although it may be desirable to directly treat lung and bronchi. In such administration inhalation methods are employed. For administration by inhalation, the compounds of the present invention are conveniently delivered in the form of an aerosol spray presentation from pressurized packs or nebulisers. The preferred delivery system for inhalation is a metered dose inhalation (MDI) aerosol, which may be formulated as a suspension or solution of the compound in suitable propellants, such as fluorocarbons or hydrocarbons.

Although the compound of the present invention may be employed as tablets, capsules, topical compositions, insufflation powders, suppositories and the like, the solubility of the compounds of the present invention in water and aqueous media render them adaptable WO 97/17365 PCT/US96/17715 13 for use in injectible formulations and also in liquid compositions suitable for aerosol sprays.

The invention is further described in connection with the following non-limiting examples.

EXAMPLE 1 Part A. Preparation of Compound F Compound E (15.9 g, 89 area% pure, 3.4 wt% water, 0.0128 mol) was added to dry THF (0.64 L) and the suspension was dried to <10 mol% water by refluxing through a bed of 3A molecular sieves. Additional dry THF was added to reconstitute the mixture to the original volume and the suspension was cooled to 4 0 C with an ice/water/methanol bath.

Neat BH3*SMe2 (10.91 g, 0.144 mol) was added over ten minutes and the reaction mixture was maintained at 0-4 0 C. The reaction progress was monitored by HPLC until the ratio of starting material to product was 1:1 indicating the end of the reaction (3.5 At 4 hours, the mixture was cooled to -12 0 C and slowly quenched with 2N HC1 (0.036 This solution was diluted to 1.14 L with water. The assay yield of Compound F was 6.60 g The quenched solution was diluted to 4 L and loaded onto a medium-pressure column of LiChroprep RP-C18 adsorbent (158 g).

After loading, the column was washed with 1.2 L water and the amine was eluted with 1.9 L of 1:4 v/v acetonitrile/water, and then 0.38 L of 1:3 v/v acetonitrile/water.

The rich cuts 80 area%) were combined and diluted with water to a 1:7.3 v/v acetonitrile/water solution (1.70 L total). This mixture was loaded to the same column described above, and the column was washed with 0.57 L water. The desired compound was eluted with 0.57 L methanol. The rich cut fractions 85 area%) were combined and concentrated by rotary evaporation and static high vacuum to give 6.81 g (87 wt% pure, 6.8 wt% water) containing 5.92 g WO 97/17365 PCT/US96/17715 14 of Compound F (where R1 is dimethyltridecyl) hydrochloride salt for an isolated yield of 43%.

1 H NMR (400 MHz, CD30D): 5 7.12 2H), 6.75 2H), 5.18 (d, 1H), 4.97 1H), 1.19 3H), 0.89 3H), 0.86 6H).

Mass spectrum (FAB) m/z 1058.

Part B. Preparation of Compound G A solution of Compound F as the hydrochloride salt (41.4g, 0.038 mol), ethanolamine hydrochloride (100 g, 1.0 mol), and hydrochloric acid in 1,4-dioxane (11.4 ml, 0.046 mol) in anhydrous dimethylsulfoxide (200 ml) was stirred at ambient temperature under a nitogen atmosphere for a period of 66 hours.

HPLC analysis (41% acetonitrile/water/0.1% trifluoroacetic acid) showed 6% unreacted Compound F (tR 7.9 min) and 56% Compound G (tR 4.2 min). The reaction mixture was diluted to 1 liter with water and filtered through a bed of filter aid. Pump-injection of the filtrate onto a preparative C18 HPLC column was followed by elution with 5-15% acetonitrile/water/0.1% acetic acid in 5% step gradients.

The product-containing fractions (40% acetonitrile/water/0.1% trifluoroacetic acid, tR 4.2 min) were combined and diluted 10% with water. Compound G was isolated by C18 solid-phase extraction eluting with methanol. Removal of the methanol in vacuo provided a syrup which was dissolved in 50% CH3CN/water and lyophilized to give 18.1 g of Compound G as a mixture of hydrochloride and acetate salts suitable for use in the subsequent deacylation step. HPLC purity Rechromatography of a portion of the mixed salt followed by anion exchange to the dihydrochloride provided Compound G as the dihydrochloride salt. HPLC purity 99%.

IH NMR (400 MHz, CD30D): 8 7.12 2H), 6.77 2H), 5.18 (d, 1H), 3.14 2H), 3.09 2H).

WO 97/17365 PCT/US96/17715 Anal. (C 52

H

89 C1 2

N

9 0 16 C, H, N, Cl. HRMS (FAB) m/z (M+1) Calculated for C 52

H

88

N

9 0 16 1094.6348 Found 1094.6338.

Part C. Deacvlation of Compound G to Give Triamine H Compound G was enzymatically deacylated as follows: i. Preparation of C. acidovorans cells To establish a seed culture of C. acidovorans ATCC 53942, a loopful of cells from an LB agar slant of the organism was used to inoculate 50 mL of Lauria-Bertani (LB) medium in a 250-mL unbaffled Erlenmeyer flask. The flask was incubated at 25 0 C with agitation at 220 rpm for about 18 hours and 0.1 mL of this culture was used to inoculate each of three 250-mL Erlenmeyer flasks containing 50 mL of LB medium.

After incubating under the same conditions for 18 hours, 20 mL of the grown cells were used to inoculate 15 liters of LB medium in each of six 23-liter fermentors. The fermentors were operated at 30 0 C with agitation at 400 rpm and aeration at 7.4 L/min for 18 hours. Cultures from three fermentors were combined, washed and concentrated to 11 liters in 50 mM potassium phosphate buffer, pH 7.5 using a hollow fiber ultrafiltration system.

ii. Deacylation of Compound G Deacylation was set up in two 15-liter Microferm fermentors (New Brunswick Scientific). In one vessel 10 g of Compound G dissolved in 250 mL of distilled water was added to liters of the washed and buffered C. acidovorans cells while in the second vessel 8.2 g of Compound G dissolved in 200 mL of distilled water was added to 4 liters of the cells. Addition of Compound G to each vessel was accomplished with a pipette over a period. To reduce foaming, about 2 mL and 1.5 mL of P2000 were added to vessels A and B respectively. Each fermentor was operated with stirring at 100 rpm, aeration at 1.5 L/min and temperature at 37 0

C

for the first 2.5 hours and 30 0 C for the last 21.5 hours. After 24 hours, WO 97/17365 PCT/US96/17715 16- HPLC analyses indicated that deacylation was 100% complete in each vessel. The contents of both vessels were combined and the cells were removed by hollow fiber filtration. HPLC analysis confirmed that the filtrate contained Compound H, the nucleus of Compound G.

iii. Isolation of Compound H The deacylation broth from the procedure above was proceessed by loading 17 L broth onto 4x120 g resin columns (BioRad AG50W-X8, H+ form). The columns were each washed with 1 L of water to pH 7.0 prior to loading the phosphate-buffered broth. Each loading was followed by 1 L water wash and compound H was then eluted with 0.15N NH4OH. The product-containing fractions (approximately 2L) were combined and lyophilized to give 10.3 g of crude Compound H (free amine). This was then slurried in 100 mL of water, cooled to 0 OC and 100 mL of 2% CF 3

COOH/H

2 0 was added eq, pH This was then loaded onto a 50 g RP-C18 plug (rinsing with 0.1% aqueous trifluoroacetic acid). Lyophilization then gave 16.2 g of Compound H as the tri-TFA salt pure). A small portion was further purified on an Rx-C18 column (21.2 mm x 25 cm) using CH3CN/H 2 0 CF3COOH). Elution at a flow rate of ml/min was monitored by UV at 220 and 277 nm. The productcontaining fractions, as determined by analytical C18-HPLC (4.6 mm x cm, 3.5% CH3CN/H 2 0 CF3COOH) at 1.5 ml/min, tR 4.9 min) were combined and lyophilized to give >98% pure Compound H.

1H NMR (500MHz,CD 3 0D) 8 7.13 J=8.4 Hz, 2H), 6.75 J=8.7 Hz, 2H), 5.24 J=3.2 Hz, 1H), 5.01 J=2.7 Hz, 1H), 4.42 (dd, and 11.2 Hz, 1H), 4.38 J=7.8 Hz, 1H), 3.98 (dd, 3.1 and 11.1 Hz, 1H), 3.15 2H), 3.05 (t,J=7.3 Hz, 2H), 2.43(dd, J=6.9 and 13.0 Hz, 1H), 1.26 J=6.2 Hz, 3H).

ESI-MS 857.6 WO 97/17365 PCT/US96/17715 17iv. Preparation of Compound I from Compound H Step a.

To a stirred solution of Compound H (16.0 g pure], 0.0094 mol) in anhydrous dimethylformamide (75 mL) was added triethylamine (9.3 mL, 0.067 mol), followed by benzyl-4-nitrophenyl carbonate (5.11 g, 0.019 mol), and this was stirred for 20 minutes.

HPLC indicated incomplete formation of Compound J (analytical Cl 8- HPLC 30% acetonitrile/water/0.1 trifluoroacetic acid, tR 3.7 min).

Additional carbonate (1.46 g, 0.005 mol) was added and HPLC indicated mainly Compound J as product. The pentaflourophenyl 6-nheptyloxy-2-napthoate (6.65 g, 0.015 mol) was then added and stirred for 18 hours. HPLC indicated consumption of Compound J and formation of acylated product, Compound K. The reaction was diluted with water (75 mL) and loaded onto a RP C18 flash column (50 g) packed in 30% CH 3

CN/H

2 0; rinsed with 6 x 50 mL of

CH

3

CN/H

2 0; and lastly 10 x 50 mL 100% methanol. The productcontaining methanol fractions were combined and concentrated to give 11.1 g of crude Compound K (80% pure). A small portion of Compound K was further purified on Zorbax Rx-C18 (21.2 x 250 mm) eluting with 70% CH 3

CN/H

2 0 at a flow rate of 10 mL/min. The product-containing fractions, as determined by HPLC (75% A/B, tR 3.1 min),were combined and lyophilized to give 98% pure Compound

K.

'H NMR (CD 3 0D) 5 8.32 1H), 7.85 J=7.6 Hz, 1H), 7.74 (d, J=8.9 Hz, 1H), 7.66 J=8.7 Hz, 1H), 7.10-7.30 14H), 6.75 (d, Hz, 2H), 5.29 1H), 4.99 2H), 4.93 4H), 3.96 (dd, J=3.1 and 11.1 Hz, 1H), 3.61 1H), 3.52 1H), 3.40 1H), 2.43 (dd, J=6.9 and 13.0 Hz, 1H), 1.84 2H), 1.22 J=6.1 Hz, 3H), 0.92 (t, J=6.9 Hz, 3H).

ESI-MS (M+TFA)- 1505 WO 97/17365 PCT/US96/17715 18 Step b.

To a stirred suspension of Compound K (11.0 g [80% pure], 0.006 mol) in CH 3 OH (200 mL) and CH 3 COOH (20 mL) was added 10% Pd/C. This was then subjected to an atmosphere of hydrogen (balloon pressure) and allowed to stir for 3 hours. HPLC indicated complete deprotection and catalyst was filtered through a pad of diatomaceous earth, rinsing with CH30H, and concentration gave crude Compound I. This was then dissolved in water (150 mL) and loaded onto Delta Prep C18 for purification. A step-gradient chromatography from 10-30% acetonitrile/water/0.1% acetic acid was used and lyophilization of the appropriate fractions as determined by HPLC acetonitrile/water/0.1% trifluoroacetic acid, tR 4.7 min) gave 6.2 g of Compound I as the di-acetate salt. This was then dissolved in water (200 mL) and applied to a BioRad AG2-X8, Cl- resin column (70 g); elution with H 2 0 and subsequent lyophilization gave 5.8 g of Compound I as the dihydrochloride salt.

'H NMR (CD30D) 8 8.36 1H), 7.80-7.90 3H), 7.28 J=1.8 Hz, 1H), 7.21 (dd, J=2.2 and 9.0 Hz, 1H), 7.13 J=8.5 Hz, 2H), 6.76 J=8.5 Hz, 2H), 5.29 1H), 5.03 J=3.0 Hz, 1H), 4.94 (d, J=6.6 Hz, 2H), 4.76 (dd, J=4.7 and 12.9 Hz, 1H), 4.13 3H), 3.87 (t, J=6.8 Hz, 2H), 3.18 2H), 3.10 J=5.5 Hz, 2H), 2.45 (dd, J=7.0 and 12.7 Hz, 1H), 1.53 2H), 1.22 J=6.2 Hz, 3H), 0.92 J=6.9 Hz, 3H).

Anal. (C 54

H

79

C

2

N

9 0 1 7 C, H, N, Cl. ESI-MS 1094.6 EXAMPLE 2 Part A. Deacvlation of Pneumocandin Bo The preparation of the deacylating enzyme is first described. A loopful of Comamonas acidovorans ATCC 53942 is inoculated into 50 milliliters of Luria Bertani medium of the following WO 97/17365 PCT/US96/17715 19composition: per liter Bacto-Trypton, 10 g; Bacto-Yeast Extract, 5 g and sodium chloride 10 g and solidified with 2 percent agar and incubating for 24 hours with shaking to obtain a seed culture. Cells for the deacylation are then grown by diluting a 50 milliliter portion of seed culture 1:500 into fresh Luria-Bertani medium and incubating at oC with shaking for 16 hours. Cells are then harvested by centrifugation, washed by resuspending in 1 percent sodium chloride and centrifuged and then resuspended in potassium phosphate buffer at pH A solution of pneumocandin Bo (Compound E) in dimethylsulfoxide is then added to a stirred suspension of the cells of C.

acidovorans thus obtained and the mixture maintained at 37 oC for 18 hours to obtain the deacylated product, Compound L, which is recovered in the supernatant after centrifugation.

Compound L may be isolated by adsorbing the supernatant on HP-20 resin with water, eluting with methanol and concentrating the eluates. The eluates are combined, diluted with water, charged to a preparative HPLC system equipped with a Whatman Partisil 10 SCX column and then eluted with 0.01M potassium phosphate (pH=6) buffer and monitored via UV at 210 nm. Cuts rich in the deacylated product were combined and the resulting mixture absorbed and eluted from HPresin with methanol to remove buffer salts and to obtain Compound L with a molecular weight of 826.

Part B. Reacylation of Compound L to Give Compound M Pentafluorphenyl 6-n-heptyloxy-2-naphthoate is prepared as follows. To a suspension of 6-n-heptyloxy-2-naphthoic acid (10.5 mmol) and dicyclohexylcarbodiimide (10.5 mmol) in ethyl acetate at 0°C is added pentafluorophenol (11.5 mmol). The mixture is stirred at °C for a period of 18 h. Any precipitate is removed by filtration.

The filtrate is washed twice with water and brine and dried with magnesium sulfate. Removal of the ethyl acetate in vacuo gives the desired pentafluorphenyl 6-n-heptyloxy-2-naphthoate.

WO 97/17365 PCTIUS96/17715 Pentafluorophenyl 6 -n-heptyloxy-2-naphthoate (1.1 mmol) prepared as above is added to a solution of Compound L from Part A mmol) in anhydrous N,N-dimethylformamide. The resulting solution is stirred at room temperature for a period of 12 to 24 h. The mixture is subsequently diluted with water. C18-Flash chromatography of the mixture eluting with 30-100% CH3CN/H20 in 10% step gradients followed by lyophilization of the product-containing fractions as determined by analytical HPLC (Zorbax RX-C18, UV detection at 210 nm) gives the desired Compound M with a molecular weight of 1095.2 Part C. Reduction of Compound M to Give Compound N A reaction vessel is outfitted with an addition funnel charged with 3-4 angstrom molecular sieves and a reflux condenser above the addition funnel. Within the reaction vessel, Compound M is suspended in dry tetrahydrofuran and heated to reflux under a nitrogen atmosphere. The mixture is refluxed for 12-24 hours, then cooled to 0°C. Borane-methylsulfide complex (10-20 equivalents) is added dropwise over 1-2 hours. The mixture is stirred at 0°C for 2-5 hours and then carefully quenched with 2N hydrochloric acid. Stirring is continued for 1 hour after the final addition of hydrochloric acid. The mixture is concentrated to 40-50 percent of the original volume and then diluted with 6-7 volumes of distilled water. Purification by preparative reverse phase HPLC (DeltaPak C18, acetonitrile/water/0.1% acetic acid, UV detection at 210 nm) and subsequent lyophilization of the appropriate fractions, as determined by analytical HPLC, gives the desired compound. The hydrochloride salt is prepared by passage of an aqueous solution of the residue through an anion exchange column (BioRad AG2-X8, Cl- resin) to give the desired Compound N with a molecular weight of 1117.6.

WO 97/17365 PCT/US96/17715 -21 Part D. Aminoethyletherification of Compound N to Compound I To a solution of the cyclohexapeptidyl propanolamine compound (0.2 mmol) in DMSO (8 mL) containing 2 -aminoethanol as the hydrochloride salt (20 mmol, 100 eq based on the cyclohexapeptide propanolamine) is added 4M HCI in dioxane (0.4 mmol) and the mixture is stirred at room temperature for two to seven days. The progress of the reaction may be monitored by HPLC using a 0.5 cm X cm analytical Zorbax C18 column eluted with a mixture of acetonitrile and water containing 0.1 trifluoroacetic acid (typically the solvent was from 10-50% acetonitrile). When the reaction is judged to be complete, the reaction mixture is diluted with five to ten volumes of water and the resulting mixture applied to a reverse phase flash silica gel column using Lichroprep C18 (particle size 40-63 microns). The mixture is then eluted initially with 5%-15% acetonitrile in water to remove the excess DMSO and 2-aminoethanol hydrochloride, then with 10-50% acetonitrile in water to elute the product. Fractions containing the desired product are combined, concentrated and lyophilized to afford the product. Subsequent exchange for chloride ion may be accomplished by dissolving the residue in water and passing through a BioRad AG2-X8, Cl- resin column to give the desired Compound I as the dihydrochloride with a molecular weight of 1197.2.

22 Example 3 Comparative Data Indicating the Efficacy of Compound I of the Present Invention With Respect to Compound If disclosed in EP 0 535 967 Compound I of the structure

H

2

N

00C 7

H

1

S.

S S

S

S S

S

S..

a a S

S.

a a

S.

S. *a S S

S

S

was compared with Compound 11 of the structure 1/ 7:~i ''ii 217 I N:\LIBAA01 49 1:tab The compounds were assessed for acute toxicity by preparing an aqueous solution of the test compound in distilled water and administering the compound to three rates by intravenous injection via the tail vein. Signs of histamine release were noted by observing vasodilation of capillary veins in the feet and snout areas, and by signs of ataxia and respiratory distress.

The response was graded as follows: 0 no response, very minor response, minor response, moderate response and severe response.

Death was also recorded.

The results for the compounds are as noted in the table below: Compound Dose Vasodilation Ataxia Respiration mg/kg Compound II 5 0 0 0 a Compound I 5 0 0 0 10 0 (2 rats) 0 (2 rats) 0 (1 rat) (1 rat) r 1/3 rats died within one hour of dosing.

Compound I produced only a minor response with respect to vasodilation and ataxia at 10mg/kg in only one of three rates and no response in two of three rats; SCompound I caused no respiratory distress in any of the rats; 15 Compound II produced a moderate to mild response in all three categories and all S* three rats at 10mg/kg and a severe response in all three categories and all three rats at Based on the results shown above, Compound I illicits a much less severe histamine-like response than Compound II while showing comparable antifungal effectiveness.

N:\IBAAI 491tab [N:\LIBAAIO 1491:tab

Claims (13)

1. A compound of the formula: rNNH

2 OH O O 0 OH H 2 N 0 NN 0 H NH 0 H 0 HO0 N-H H H N 0 0 OH H O O HO O OH H(0 0H H (NH3O\ OH 5 or an acid addition salt thereof. A compound as defined in claim 1 wherein the acid addition salt is an acetate, trifluoroacetate, chloride, bromide, tartrate, succinate, glutamate, sulfate, phosphate or pamoate.

3. A compound as defined in claim 2 wherein the acid addition salt is trifluoroacetate or chloride.

4. A method of preparing a cyclohexapeptidyl bisamine derivative, the method being substantially as hereinbefore described with reference to Compound I of the examples.

5. A cyclohexapeptidyl bisamine derivative being Compound I substantially as hereinbefore described with reference to the examples.

6. A pharmaceutical composition including or consisting of an effective amount of at least one compound as defined in any one of claims 1 to 3 or 5, together with a pharmaceutically acceptable carrier, diluent or adjuvant therefore.

7. A method for the treatment or prophylaxis of a fungal infection in a mammal, which method includes or consists of administering to said mammal an effective amount of at least one compound as defined in any one of claims 1 to 3 or 5, or of a composition as defined in claim 6.

8. A method for the treatment or prophylaxis of an infection caused by Candida sp. in a mammal, which method includes or consists of administering to said mammal an effective amount of at least one compound as defined in any one of claims 1 to 3 or 5, or of a composition as defined in claim 6. [N:\LIBAA]O1491:TAB

9. A method for the treatment or prophylaxis of an infection caused by Aspergillus sp. in a mammal, which method includes or consists of administering to said mammal an effective amount of at least one compound as defined in any one of claims 1 to 3 or 5, or of a composition as defined in claim 6.

10. A method for the treatment or prophylaxis of an infection or condition caused by Pneumocyctis carinii in a mammal, which method includes or consists of administering to said mammal an effective amount of at least one compound as defined in any one of claims 1 to 3 or 5, or of a composition as defined in claim 6.

11. A compound as defined in any one of claims 1 to 3 or 5, or a composition as defined in claim 6 when used for the prophylaxis or treatment in a mammal of a member selected from a group consisting of fungal infections, infections caused by Candida sp, infections caused by Aspergillus sp and infections or conditions caused by Pneumocyctis carinii.

12. Use of a compound as defined in any one of claims 1 to 3 or 5, or a composition as defined in claim 6 for the manufacture of a medicament for the prophylaxis or treatment in a mammal of a member selected from a group consisting of fungal infections, infections caused by Candida sp, infections caused by Aspergillus sp and infections or conditions caused by Pneumocyctis carinii.

13. A medicament for the prophylaxis or treatment in a mammal of a member 20 selected from a group consisting of fungal infections, infections caused by Candida sp, S: infections caused by Aspergillus sp and infections or conditions caused by Pneumocyctis carinii when prepared by the use as defined in claim 12. Dated 17 May 1999 25 Merck Co., Inc. Patent Attorneys for the Applicant/Nominated Person SPRUSON&FERGUSON [N:\LIBAA]01491:TAB