AU682970B2 - Antiviral dendrimers - Google Patents

Description

PCT/AU 9 5 0 0 3 5 0 96o09,p:A\pjcm,,PM6239,, RECEIVED 0 9 OCT 1996 -1- ANTIVIRAL DENDRIMERS.

FIELD OF THE INVENTION This invention relates to antiviral agents, and in particular it relates to dendrimers which have been found to have significant antiviral activity against human immunodeficiency virus (HIV) and other enveloped viruses.

BACKGROUND OF THE INVENTION It has been established that certain sulfonated polysaccharide compounds have antiviral activity when screened against HIV, however these compounds are relatively unstable and accordingly large amounts of these compounds are required to obtain effective antiviral effects. In addition, many of these compounds, including heparin and dextran sulfate for example, are potent anticoagulants and because of this activity they are not particularly suited for clinical use as antiviral agents.

The present invention provides a new class of antiviral agents based on a particular type of polymer referred to herein as a "dendrimer", which have substantial antiviral activity against HIVl and HIV2, CMV and HSV, and which have substantially no anticoagulant activity. These compounds are therefore well suited for prophylactic and therapeutic use as antiviral agents in humans.

SUMMARY OF THE INVENTION According to the present invention, there is provided an antiviral compound comprising a dendrimer having a plurality of terminal groups wherein at least one of said terminal groups has an anionic- or cationic-containing moiety, other than a 2-thiosialic acid Smoiety, bonded thereto.

AMENDED

SHEET

IPEA/AU

WO 95/34595 PCT/AU95/00350 -2- Such a dendrimer is referred to herein as an "anionic or cationic dendrimer", and this term is used throughout this specification and the claims which follow to include not only the dendrimers per se, but also their pharmaceutically or veterinarily acceptable salts, for example the alkaline metal or alkaline earth metal salts such as the sodium, potassium or calcium salts.

DETAILED DESCRIPTION OF THE INVENTION Dendrimers are macromolecular highly branched compounds formed by reiterative reaction sequences starting from an initial, core molecule with successive layers or stages being added in successive "generations" to build up a three-dimensional, highly ordered polymeric compound. A generalised dendrimer structure is shown in Figure 1.

Dendrimers are characterised by the following features: i an initiator core(I) which may have one or more reactive sites and be point-like or of significant size so as to effect the final topology of the dendrimer; ii layers of branched repeating units attached to the initiator core; iii functional terminal groups(Z) attached to the surface of the dendrimer.

The present invention uses dendritic structures a frameworks for the attachment of ionic moieties; the invention is not limited to the spherical dendrimers described in detail herein but can be based on any dendritic structure. The variety of dendrimers in both shape and constitution are well known to persons skilled in the art.

The preparation of dendrimers is well known, and is described by way of example in U.S. Patents Nos. 4,289,872 and 4,410,688 (describing dendrimers based on layers of lysine units), as well as U.S. Patents Nos. 4,507,466, 4,558,120, 4,568,737 and 4,587,329 (describing dendrimers based on other units including polyamidoamine or PAMAM dendrimers). The dendrimers disclosed in these US patents are described as being suitable for uses such as surface modifying agents, as metal chelating agents, as demulsifiers or oil/water emulsions, wet strength agents in the manufacture of paper, and as agents for modifying viscosity in aqueous formulations such as paints. It is also suggested in U.S. Patents Nos. 4,289,872 and 4,410,688 that the dendrimers based on lysine units can be used as substrates for the preparation of pharmaceutical dosages.

PCT/AU 9 5 0035 0 961009,p:opcn,PM6,239,3 RECEIVED 0 9 OCT 199 -3- International Patent Publications Nos. WO 88/01178, WO 88/01179 and WO 88/01180 disclose conjugates in which a dendrimer is conjugated or associated with another material such as a carried pharmaceutical or agricultural material. These patent publications together with the U.S. patents mentioned above contain a broad disclosure of various dendrimers and processes for the preparation thereof, and the disclosure of each of these publications is incorporated herein by reference.

The term "dendrimer" as used herein is to be understood in its broadest sense, and to include within its scope all forms and compositions of these dendrimers as disclosed in Patent Publications Nos. WO 88/01178, WO 88/01179 and WO 88/01180. The term also includes linked or bridged dendrimers as disclosed in these patent publications.

The preferred dendrimers of the present invention comprise a polyvalent core covalently bonded to at least two dendritic branches, and preferably extend through at least two generations. Particularly preferred dendrimers are polyamidoamine (PAMAM) dendrimers, PAMAM (EDA) dendrimers and polylysine dendrimers.

In accordance with the present invention, at least one, and preferably a substantial number, of the terminal groups on the surface of the dendrimer has an anionic- or cationiccontaining moiety covalently bonded thereto. The branches of the dendrimer may terminate in amino groups or other functional reactive groups such as OH, SH, or the like, which subsequently can be reacted with the cationic and anionic moieties forming the outer layer of the dendrimer. Where the terminal groups of the dendrimer are amine groups, the anionicor cationic-containing moiety may be bonded to the dendrimer by a variety of functional groups including amide and thiourea linkages. Preferred anionic- or caternd-containing moieties which may be bonded to the terminal groups of the dendrimer include sulfonic acidcontaining moieties, carboxylic acid-containing moieties other than 2-thiosialic acid moieties, trimethylammonium-containing moieties and polyamino-macrocycle-containing mitties.

Suitable anionic- and cationic-containing moieties which may be bonded to the amino or other terminal groups of deadrimers include, by way of example, the following SAMENDED

SHEET

IPEA/AU

II WO 95/34595 PCr/AU95100350 -4groups (in which n is zero or a positive integer, more particularly n is zero or an integer of from 1 to

-NH(CR

2 Si

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2 NMeS)n YS0Na ,,,SqNa .*XV>(KSSNa 0 0 /jj'Vk'ONa I COOH

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EN N N P WO 95134595 PCIAU95/00350 Particular moieties which may be bonded to the terminal groups of the dendrimer in accordance with this invention include alkyl sulfonic acid groups; sulfoacetamide groups; sulfosuccinamic acid groups; N-sulfoalkyl succinamide groups, such as N-(2sulfoethyl)succinamide groups; aryl or heteroaryl thioureas substituted with one or more sulfonic acid groups, such as 4-sulfophenylthiourea groups, 3,6-disulfonapthylthiourea groups, 4-sulfonapthylthiourea groups, 3,5-disulfophenyl thiourea groups and 3,6,8trisulfonapthylthiourea groups; aryl or heteroaryl amides substituted with one or more sulfonic acid, sulfoalkyl, sulfoalkoxy, sulfoalkylamino or sulfoalkylthio groups, such as 4-(sulfomethyl) benzamide groups or 4-sulfobenzamide groups; aryl or heteroaryl alkanamides substituted withone or more sulfonic acid groups, such as N-(4-sulfophenyl) propanamide groups; aryl or heteroaryl ureas substituted with one or more sulfonic acid groups, such as 4-sulfophenyl urea groups; N,NN-trimethyl derivatives of amino acids, such as N,N,N-trimethylglycinamide groups; aryl or heteroarylamides substituted with one or more trialkylamino, trialkylaminoalkyl, trialkylaminoalkyloxy, trialkylaminoalkylamino or trialkylaminoalkylthio groups, such as 4-trimethylammonium benzamide or 4-(trimethylammonium methyl) benzamide groups; N-(2-acetoxyethyl)- N,N-(dimethylammonium)methylcarboxamide groups; guanidino groups; 4-carboxy-3hydroxybenzylamine groups; or macrocyclic polyamino groups containing onc or more macrocyclic rings connected through an alckyl or aryl spacer moiety to the terminal group of the dendrimer, such as 4-([1,4,8,11 -tetraazacyclotetradecane]methyi)benzamide groups.

The anionic or cationic dendrimers of this invention may be prepared by standard chemical methods which are well known to persons skilled in this art. Suitable methods are described by way of example in Examples 1 to 20 below.

As previously described, the anionic or cationic dendrimers of the present invention have been found to exhibit significant antiviral activity, particularly against HIV. Accordingly, these anionic or cationic dendrimers are useful in prophylactic and therapeutic treatment of viral infections, for example infections by HIV1 and HIV2 and other enveloped viruses including flaviviruses such as Hepatitis B and Hepatitis C, Bovine Viral Diarrhoea Virus, Human Influenza Virus A and B, Rhinovirus, Human Parainfluenza Virus, Respiratory Syncytial Virus (RSV), Varicella Zoster Virus (VZV), I WO 95/34595 PCT/AU95/00350: -6- Human Cytomegalovirus (CMV), Epstein Barr Virus (EBV), Human Papilloma Virus (HPV), Adenovirus-8, Herpes Simplex Virus (HSV) type 1 and 2, Measles Virus, and Vesicular Stomatitis Virus (VSV).

Thus, in another aspect the present invention provides a pharmaceutical or veterinary composition for prophylactic or therapeutic antiviral treatment of a human or non-human animal, which comprises an anionic or cationic dendrimer as broadly described above, in association with at least one pharmaceutically or veterinarily acceptable carrier or diluent.

The formulation of such compositions is well known to persons skilled in this field. Suitable pharmaceutically acceptable carriers and/or diluents include any and all conventional solvents, dispersion media, fillers, solid carriers, aqueous solutions, coatings, antibacterial and antifungal agents, isotonic and absorption delaying agents, and the like.

The use of such media and agents for pharmaceutically active substances is well known in the art, and it is described, by way of example, in Remington's Pharmaceutical Sciences, 18th Edition, Mack Publishing Company, Pennsylvania, USA. Except insofar as any conventional media or agent is incompatible with the active ingredient, use thereof in the pharmaceutical compositions of the present invention is contemplated.

Supplementary active ingredients can also be incorporated into the compositions.

It is especially advantageous to formulate compositions in dosage unit form for ease of administration and uniformity of dosage. Dosage unit form as used herein refers to physically discrete units suited as unitary dosages for the human subjects to be treated; each unit containing a predetermined quantity of active ingredient calculated to produce the desired therapeutic effect in association with the required pharmaceutical carrier and/or diluent. The specifications for the novel dosage unit forms of the invention are dictated by and directly dependent on the unique characteristics of the active ingredient and the particular therapeutic effect to be achieved, and the limitations inherent in the art of compounding such an active ingredient for the particular treatment ~e d I3 i WO 95/34595 PCT/AU95/00350 -7- In another aspect, the present invention provides a method for prophylactic or therapeutic treatment of an HIV or other viral infection in a human or non-human animal, which comprises administering to said human or animal a prophylactic- or therapeuticantiviral-effective amount of an anionic or cationic dendrimer as broadly described above.

In yet another aspect, this invention provides the use of a prophylactic- or therapeutic-antiviral-effective amount of an anionic or cationic dendrimer as broadly described above in the prophylactic or therapeutic treatment of, or in the manufacture of a medicament for prophylactic or therapeutic treatment of an HIV or other viral infection in a human or non-human animal.

A variety of administration routes are available. The particular mode selected will depend, of course, upon the particular condition being treated and the dosage required for therapeutic efficacy. The methods of this invention, generally speaking, may be practised using any mode of admirdstration that is medically acceptable, meaning any mode that produces therapeutic levels of the active component of the invention without causing clinically unacceptable adverse effects. Such modes of administration include oral, rectal, topical, nasal, transdermal or parenteral subcutaneous, intramuscular and intravenous) routes. Formulations for oral administration include discrete units such as capsules, tablets, lozenges and the like. Other routes include intrathecal administration directly into spinal fluid, direct introduction such as by various catheter and balloon angioplasty devices well known to those of ordinary skill in the art, and intraparenchymal injection into targeted areas.

The compositions may co \veniently be presented in unit dosage form and may be prepared by any of the methods well known in the art of pharmacy. Such methods include the step of bringing the active component into association with a carrier which constitutes one or more accessory ingredients. In general, the compositions are prepared by uniformly and intimately bringing the active component into association with a liquid carrier, a finely divided solid carrier, or both, and then, if necessary, shaping the product.

c~ ~wR~ WO 95/34595 PCT/AU95/00350 -8- Cc positions of the present invention suitable for oral administration may be presented as discrete units such as capsules, cachets, tablets or lozenges, each containing a predetermined amount of the active component, in liposomes or as a suspension in an aqueous liquor or non-aqueous liquid such as a syrup, an elixir, or an emulsion.

Compositions suitable for parenteral administration conveniently comprise a sterile aqueous preparation of the active component which is preferably isotonic with the blood of the recipient. This aqueous preparation may be formulated according to known methods using those suitable dispersing or wetting agents and suspending agents. The sterile injectable preparation may also be a sterile injectable solution or suspension in a non-toxic parenterally-acceptable diluent or solvent, for example as a solution in polyethylene glycol. Among the acceptable vehicles and solvents that may be employed are water, Ringer's solution and isotonic sodium chloride solution. In addition, sterile, fixed oils are conventionally employed as a solvent or suspending medium. For this purpose, any bland fixed oil may be employed including synthetic mono-or di-glycerides.

In addition, fatty acids such as oleic acid find use in the preparation of injectables.

Other delivery systems can include sustained release delivery systems. Preferred sustained release delivery systems are those which can provide for release of the active component of the invention in sustained release pellets or capsules. Many types of sustained release delivery systems are available. These include, but are not limited to: erosional systems in which the active component is contained within a matrix, and (b) diffusional systems in which the active component permeates at a controlled rate through a polymer. In addition, a pump-based hardware delivery system can be used, some of which are adapted for implantation.

The active component is administered in prophylactically or therapeutically effective amounts. A prophylactically or therapeutically effective amount means that amount necessary at least partly to attain the desired effect, or to delay the onset of, inhibit the progression of, or halt altogether, the onset or progression of the particular condition being treated. Such amounts will depend, of course, on the particular condition being treated, the severity of the condition and individual patient parameters including WO 95/34595 PCT/AU95/00350 -9age, physical condition, size, weight and concurrent treatment. These factors are well known to those of ordinary skill in the art and can be addressed with no more than routine experimentation. It is preferred generally that a maximum dose be used, that is, the highest safe dose according to sound medical judgement. It will be understood by those of ordinary skill in the art, however, that a lower dose or tolerable dose may be administered for medical reasons, psychological reasons or for virtually any other reasons.

Generally, daily oral doses of active component will be from about 0.01 mg/kg per day to 1000 mg/kg per day. Small doses (0.01-1 mg) may be administered initially, followed by increasing doses up to about 1000 mg/kg per day. In the event that the response in a subject is insufficient at such doses, even higher doses (or effective higher doses by a different, more localised delivery route) may be employed to the extent patient tolerance permits. Multiple doses per day are contemplated to achieve appropriate systemic levels of compounds.

Throughout this specification and the claims which follow, unless the context requires otherwise, the word "comprise", or variations such as "comprises" or "comprising", will be understood to imply the inclusion of a stated integer or group of integers but not the exclusion of any other integer or group of integers.

Further features of the present invention will be apparent from the following Examples which are included by way of illustration, not limitation of the invention. In the following Examples, PAMAM dendrimers refer to polyamidoamine dendrimers based on an ammonia core as detailed in US Patents Nos. 4,507,466, 4,558,120, 4,568,737 and 4,587,329; PAMAM (EDA) dendrimers refer to polyamidoamine dendrimers based on an ethylene diamine core; and BHAlysjlysyys, dendrimers refer to polylysine unsymmetrical dendrimers based on a benzhydrylamine core and lysine branching units as described in US Patents Nos. 4,289,872 and 4,410,688. The polyamidoamine dendrimers PAMAM 1.0, PAMAM 2.0, PAMAM 3.0, PAMAM 4.0, PAMAM 5.0 or higher generation, PAMAM 4.0 (EDA), and the polylysine dendrimers BHAlyslys 2 BHAlyslys 2 lys 4 BHAlyslys 2 lys 4 lyss and BHAlyslysalys4lysglys 1 6 BHAlyslly 4 salys4ys 1 lys 3 2 BHAlyslys 2 lys 4 ly 8 l1ys6lys 3 2 1ys64, or higher generations are ;I ~a WO 95/34595 PCT/AU9S/00350 prepared as described in U.S. Patents Nos. 4,289,872, 4,410,688, 4,507,466, 4,558,120, 4,568,737 and 4,587,329 and International Patent Publications Nos. WO 88/01178, WO 88/01179 and WO 88/01180 referred to above.

EXAMPLE 1 Reaction of polymers with 2-acrylamido-2-methyl propane sulfonic acid to give sulfonic acid terminated dendrimers.

A. PAMAM Solid sodium carbonate (0.13g; 1.0mmol) was added slowly to a stirred solution of 2-acrylamido-2-methyl propane sulfonic acid (0.41g; 2.0mmol) in water (3ml).

After the evolution of gas had ceased, the pH of the solution was 8.0. A solution of PAMAM 1.0 (0.12g; 0.33mmol) in water (Iml) was then added to the solution followed by the addition of four drops of a 40% aq. solution of benzyl trimethylammonium hydroxide. The solution was then heated under nitrogen at 600 for three days and then concentrated. The residue was purified by gel filtration (Sephadex GI0; water) and then freeze dried to give the sulfonated PAMAM 1.0 dendrimer as an off-white solid (0.51g). 'H and 13C nmr spectra showed a mixture of dialkylated and monoalkylated PAMAM 1.0 dendrimer ca.

70:30). 13C nmr (D 2 8 31.0, 31.1, 37.1, 37.7, 41.3, 48.6, 51.5, 53.1, 53.4, 55.6, 56.2, 61.2, 61.5, 178.3, 179.0, 179.8.

B. PAMAM 2.0 (Compound No. PAMAM 2.0 was reacted with 2-acrylamido-2-methyl propane sulfonic acid as described above. The crude product was purified by gel filtration (Sephadex water) and then freeze dried to give an off-white solid. 'H and 3C nmr spectra showed a mixture of dialkylated and monoalkylated PAMAM 2.0 dendrimer ca.

65:35). "C nmr (D 2 8 31.0, 31.1, 37.1, 37.7, 41.3, 48.7, 51.5, 53.4, 55.6, 56.2, 61.2, 61.5, 178.4, 179.0, 179.1, 179.6. When the above reaction was repeated omitting the benzyltrimethyl-ammonium hydroxide a similar result was obtained.

C. PAMAM PAMAM 3.0 was reacted with 2-acrylamido-2-methyl propane sulfonic acid as above except that a slight excess of sodium carbonate was used and the WO 95/34595 PCT/AU95/00350 11 benzyltrimethylammonium hydroxide was omitted. 'H and "C nmr spectra showed a mixture of dialkylated and monoalkylated PAMAM 3.0 dendrimer ca. 50:50).

3C nmr (D 2 6 31.0, 31.1, 36.9, 37.4, 41.1, 48.6, 51.5, 53.4, 55.7, 56.2, 61.1, 61.5, 178.2, 178.9, 179.0, 179.8.

D. PAMAM PAMAM 4.0 was reacted with 2-acrylamido-2-methyl propane sulfonic acid as described for PAMAM 3.0. 'H and "C nmr spectra showed a mixture of dialkylated and monoalkylated PAMAM 4.0 dendrimer ca. 35:65). 13C nmr (D20): 6 31.0, 31.1, 36.9, 37.3, 41.1, 48.5, 51.5, 53.5, 55.7, 56.2, 61.1, 61.5, 178.1, 178.9, 179.0, 179.8.

EXAMPLE 2 Preparation of sodium sulfoacetamide terminated dendrimers.

A. PAMAM A solution of 4-nitrophenyl bromoacetate (0.40g; 1.5mmol) in dry DMF (1ml) was added to a stirred solution of PAMAM 1.0 (0.18g; O.5mmol) in DMF (3ml).

The resulting yellow solution was stirred for 20 hours at room temperature, when a ninhydrin test was negative. The solution was concentrated (300/ 0. lmmHg) to give a yellow oil. This oil was partitioned between water and chloroform and the aqueous layer separated and washed with chloroform (2X) and finally with ethyl acetate. The aqueous solution was concentrated (350/ 25mmHg) to give the bromoacetylated PAMAM 1.0 dendrimer as a yellow oil (0.36g;100% 3 C nmr

(D

2 5 32.8, 33.3, 43.0, 43.5, 54.4, 174.5, 176.4.

A solution of sodium sulfite (0.2g; 1.6mmol) in water (lml) was added to a solution of the bromoacetylated PAMAM 1.0 dendrimer described above (0.36g; in water (5ml) and the solution left to stand at room temperature for eleven days. The yellow solution was concentrated to give a yellowish solid (0.60g). "C nmr (D 2 5 34.4, 43.1, 43.4, 54.0, 61.7, 171.3, 177.2.

WO 95/34595 PCT/AU95/00350 12- The above reaction sequence could be carried out without isolating the bromoacetylated dendrimer by simply adding the sodium sulfite solution to the crude aqueous extract obtained from the first reaction.

B. PAMAM Method 1: A solution of 4-nitrophenyl bromoacetate (0.18g; 0.7mmol) in dry DI (1ml) was added to a stirred solution of PAMAM 2.0 (0.10g; O. mmol) in DMF (3ml).

The resulting yellow solution was stirred for 20 hours at room temperature, when a ninhydrin test was negative. The solution was then added with swirling to water (150ml) and the mixture extracted with chloroform (3X) and ethyl acetate. A solution of sodium sulfite (0.lg; 0.8mmol) in water (Iml) was added to the crude bromoacetylated dendrimer solution and the mixture allowed to stand for three days at room temperature. The yellowish solution was then concentrated to give a yellow solid residue, which was purified by gel filtration (Sephadex water) to give the sodium sulfoacetamide terminated PAMAM 2.0 dendrimer (103mg). "C nmr (D20): 8 33.0, 35.7, 36.0, 37.7, 40.3, 43.0, 43.2, 53.4, 53.7, 56.0, 61.6, 171.2, 174.6, 178.5.

Method 2: Solid succinimidyl acetylthioacetate (67mg; 0.33mmol) was added to a solution of PAMAM 2.0 (52mg; 0.05mmol) in dry DMF (2ml) and the resulting solution stirred at room temperature for two days. The mixture was then concentrated (300/10"mmHg) to give an oily residue. The residue was partitioned between water and chloroform, and the watt r layer separated and concentrated to give a viscous oil (117mg). 'H and 13C nmr showed the oil to be a mixture of the acylated dendrimer and N-hydroxy succinimide. Gel filtration (Sephadex water) provided a pure sample of the acetylthioacetamide terminated PAMAM dendrimer (29mg). "C nmr (D20): 8 34.0, 34.2, 37.3, 43.0, 43.1, 43.3, 53.5, 54.0, 56.3, 175.4, 177.2, 177.5.

I--

WO 95/34595' PCT/AU95/00350 13 A solution of the above functionalised dendrimer in 40% aqueous formic acid (7ml) was then added to an ice cold freshly prepared solution of performic acid (1.6mmol) in formic acid (2ml). The mixture was stirred for one hour at 00 and then for twenty hours at room temperature. A small amount of activated charcoal was then added to decompose any excess peracid, the mixture stirred for minutes then filtered and concentrated to give a viscous oil. The crude product was dissolved in water, the pH adjusted to 9.0 with aqueous sodium bicarbonate and the material desalted by passage through a column of Sephadex G10. A white solid (20mg; was obtained after lyophylisation which was spectroscopically essentially the same as the material obtained by method 1. "C nmr (D 2 33.0, 38.7, 42.9, 43.0, 43.1, 53.9, 54.3, 56.5, 61.6, 171.2, 176.4, 177.0.

EXAMPLE 3 Preparation of sodium sulfosuccinamic acid terminated dendrimers A. PAMAM Solid maleic anhydride (0.1 Ig; 1. Immol) was added to a stirred solutioi of PAMAM 1.0 (0.12g; 0.33mmol) in dry DMF (3ml). The mixture became a little warm and brownish as the anhydride dissolved and the resulting solution was stirred overnight at room temperature. The solution was then concentrated (300/10.

4 mmHg) to give a viscous oil. 'H and "C nmr (D 2 0) showed complete conversion of the PAMAM 1.0 to the trisamide together with some maleic acid. "C nmr (DO2): 8 33.1, 42.8, 43.1, 54.3, 135.0, 137.1, 169.1, 171.9, 173.3.

The crude trisamide was then dissolved in water (4ml) and solid sodium sulfite (0.20g; 1.6mmol) added. The resulting solution was allowed to stand at room temperature for four days and then concentrated. 'H and 1 3 C nmr (D20) showed a 1:1 mixture of the regioisomeric sodium sulfosuccinamic acid terminated PAMAM 1.0 dendrimers together with some sulfosuccinic acid. The crude product was purified by gel filtration (Sephadex G10; water) to afford a sample of the sodium sulfosuccinamic acid terminated PAMAM 1.0 dendrimers (107mg). 1 3

C

i WO 95/34595 PCTIAU95/00350 14nmr (D 2 8 33.3, 39.6, 40.0, 42.9, 43.1, 54.0, 67.9, 69.4, 173.8, 176.3, 177.6, 181.8.

B. PAMAM A mixture of the regioisomeric sodium sulfosuccinamic acid terminated PAMAM dendrimers was prepared as described above. 3 C nmr PAMAM 2.0 maleamic acid derivative (D 2 8 32.8, 33.0, 38.7, 42.9, 53.8, 54.3, 56.5, 135.2, 136.8, 169.2, 171.9, 173.5, 174.6. "C nmr PAMAM 2.0 sodium sulfosuccinamic acid derivatives (D20): 8 37.0, 40.1, 41.1, 43.0, 43.2, 43.9, 53.0, 53.3, 55.5, 68.0, 69.4, 173.8, 177.6, 179.1, 179.5, 179.8, 182.3.

C. PAMAM 4.0 (Compound No. 14) Solid maleic anhydride (60mg; 0.6mmol) was added to a stirred solution of PAMAM 4.0 (51mg; 0.Olmmol) in dry DMF (2ml). The mixture initially became cloudy but soon gave a clear solution which was stirred overnight at room temperature. The solution was then concentrated (35°/10 4mmHg) to give a viscous oil. 'H and "C nmr (D20) showed complete conversion of the PAMAM 4.0 to the polyamide together with some maleic acid. The crude polyamide was then dissolved in water (2ml) and a solution of sodium sulfite (126mg; 1.0mmol) in water (2ml) added. The resulting solution was allowed to stand at room temperature for two days and then concentrated. 'H and "C nmr (D20) showed a mixture of the regioisomeric sodium sulfosuccinamic acid terminated PAMAM dendrimers together with some sulfosuccinic acid. The crude product was purified by gel filtration (Sephadex LH20; water) to afford a sample of PAMAM 4.0 terminated with 24 regioisomeric sulfosuccinamic acid groups (90mg). 'H nmr (D2z): 8 2.4-2.6; 2.7-3.1; 3.2-3.4; 3.9-4.0."C .amr (D20): 8 36.2; 39.8; 40.5; 43.0; 43.2; 53.5; 55.8; 68.1; 69.5; 173.8; 177.4; 177.6; 178.7; 182.3.

EXAMPLE 4 Preparation of sodium N-(2-sulfoethyl)succinamide terminated dendrimers 1- WO 95/34595 WO 9534595PCTAU95/00350 15 a. Preparation of tetrabutylammonium N-(2-sulfoethyl)succinamic acid Solid succinic anhydride (0.50g; 5.Ommol) was added to a stirred solution of tetrabutylanunonium 2-aminoethylsulfonic acid (1.83g; 5.Ommol) in dry dichloromethane (30m1). The succinic anhydride slowly dissolved and the resulting cloudy solution was stirred overnight at room temperature. The mixture was filtered and the filtrate concentrated to give a viscous oil (2.41g). 1 3 C rnr Showed complete conversion to the desired monoamide together with a small amount of succinic acid. Repeated precipitation of the product by dropwise addition of a dichioromethane solution to a large excess of diethyl ether gave tetrabutylammonium N-(2-sulfoethyl)succinamic acid as a white solid (1.762g; 76% mp 125-127C. 1 H nmr (CDC1 3 5 0.86 12h, 4xCH 3 1.28 (in, 811, 4xCH 2 1.50 (in, 8H1, 4xCH 2 2.33 (in, 2H, CH 2 COOHI), 2.44 (in, 2H1,

CH

2 CONH), 2.76 (mn, 2H1, CH 2 NHCO), 3.12 (in, 8H, 4xCH 2 3.50 (in, 211, is CH 2

SO

3 7.53 (br t, IIH, NH). 3 C nmr (CDC1 3 13.5, 19.5, 23.8, 30.1, 30.9, 35.6, 50.0, 58.5, 172.0, 174.1.

b. Preparation of tetrabutylanimonium 4-nitrophenyl N-(2-sulfoethyl)-succinainate A solution of dicyclohexylcarbodimiide (45mg; 0.22inmol) in dry dichlorolmethane (imi) was added to a stirred solution of tetrabutylammionium. sulfoethyl)succinaxnic acid (94mg; 0.20mnrol) and 4-nitrophenol (28mg; 0.2Ommol) in dichioromethanie (2m1), and the mixture stirred overnight at room temperature. The resulting suspension was filtered and the filtrate concentrated to give the crude active ester, which was used without fRther purification.

A. Preparation of sodium N-(2-sulfoethyl)succinaniide termninated PAMAM dendritners PAMAM A solution of the crude tetrabutylainmonium 4-nitrophenyl N-(2sulfoethyi)succinamate (0.3 Ommol) in dry DME (Iml) was added to a stirred WO 95/34595 PCT/AU95/00350 16solution of PAMAM 4.0 (51.5mg; 0.Olmmol) dissolved in 50% aqueous DMF (3ml) and the resulting yellow solution stirred overnight at room temperature. The mixture was then concentrated (350/ 10"mmHg) and the yellow residue partitioned between water and chloroform. The water layer was separated, washed with chloroform (2X) and ethyl acetate, and then concentrated to give a yellow oil (134mg). The crude product was converted to the sodium salt by passage through a column of Amberlite IR- 120(Na) to yield 85mg of material. This material was further purified by gel filtration (Sephadex LH20; water) to give the sodium N-(2-sulfoethyl)succinamide terminated PAMAM 4.0 dendrimer (45mg). "C nmr (D20): 8 33.2, 33.6, 35.5, 39.0, 39.5, 42.8, 43.2, 53.8, 54.1, 54.4, 56.6, 176.5, 176.9, 177.2, 178.9, 179.4.

The corresponding PAMAM 1.0 and PAMAM 3.0 dendrimers terminated with sodium N-(2-sulfoethyl)succinamide groups were similarly prepared. "C nmr PAMAM 3.0 derivative (D20): 8 33.4, 35.5, 39.0, 39.5, 42.9, 43.2, 53,8, 54.1, 54.3, 56.5, 176.4, 176.9, 177.4, 178.9, 179.4.

"C nmr PAMAM 1.0 derivative (DO0): 8 34.9, 35.5, 39.5, 42.9, 43.1, 53.7, 54.1, 179.0, 179.1, 179.3.

B. Preparation of sodium N-(2-sulfoethyl)succinamide terminated polylysine dendrimers.

BHAlyslys2ylyslyslys 1 6 Trifluoroacetic acid (1ml) was added to a suspension of BHAlyslys 2 lys 4 lysDBLs (36.5mg; 5.0pmol) in dry dichloromethane (Iml) and the resulting solution stirred at room temperature under nitrogen for two hours 'd then concentrated. The residue was dissolved in dry DMSO (2ml) and the pH adjusted to 8.5 with triethylamine. A solution of the crude tetrabutylammonium 4-nitrophenyl N-(2-sulfoethyl)succinamate (ca. 0.2mmol) in DMSO (1ml) was then added dropwise and the mixture stirred overnight at room temperature. The yellow solution was then concentrated (50°/10"smmHg) and the yellow residue partitioned between water and chloroform. The aqueous layer was separated, washed with I P WO 95/34595 PCMTAU95/00350 17chloroform (3X) and ethyl acetate, and then concentrated to give an oil (99mg).

The crude product was converted to the sodium salt by passage through a column of Amberlite IR 120(Na) to yield 81mg of material. This material was further purified by gel filtration (Sephadex LH20; water) to give the sodium N-(2-sulfoethyl)succinamideterminatedBHAlyslys 2 lys41yslysl6dendrimer(39mg).

"C nmr (D 2 6 27.0, 32.3, 35.2, 35.3, 35.6, 35.7, 39.5, 43.5, 54.1, 58.5, 131.5, 132.0, 133.3, 145.1, 177.8, 178.0, 178.4, 178.8, 178.9, 179.2, 179.7, 179.8.

The corresponding BHAlyslys 2 BHAlyslyslys 4 and BHAlyslys 2 lys 4 ,ys 8 terminated with sodium N-(2-sulfoethyl)succinamide groups were similarly prepared.

3C nmr BHAlyslys 4 lys4 derivative (D 2 8 26.9, 32.3, 35.1, 35.3, 35.6, 35.7, 39.5, 43.5, 54.1, 58.5, 131.6, 131.9, 132.2, 132.3, 133.2, 133.3, 145.0, 145.2, 177.2, 177.8, 177.9, 178.0, 178.2, 178.3, 178.6, 178.7, 178.8, 178.9, 179.2, 179.3, 179.7, 179.8.

3C nmr BHAlyslys 2 lys 4 derivative (D20): 8 26.9, 32.3, 35.1, 35.4, 35.7, 35.8, 39.5, 43.5, 54.1, 58.5, 61.8, 131.7, 132.0, 132.2, 132.3, 133.2, 133.3, 145.0, 145.1, 177.3, 178.0, 178.3, 178.4, 178.7, 178.9, 179.0, 179.3, 179.7, 179.8.

3 C nmr BHAlyslys 2 derivative (DzO): 8 26.9, 27.1, 32.2, 32.3, 34.7, 34.8, 35.1, 35.3, 35.6, 35.7, 39.5, 43.4, 54.1, 58.6, 61.8, 131.7, 131.9, 132.2, 132.3, 133.3, 144.9, 145.0, 177.7, 178.4, 178.8, 179.0, 179.3, 180.0.

EXAMPLE S Preparation of sodium 4-sulfophenylthiourea terminated dendrimers A. PAMAM 4.0 (Compound No. 1) Solid sodium 4-sulfophenylisothiocyanate monohydrate (500mg; 1.96mmol) was added to a solution of PAMAM 4.0 (300mg; 0.0582mmol) in water (10ml) and the resulting solution heated under nitrogen at 530 for two hours and then cooled.

The solution was concentrated and the yellow solid residue purified by gel filtration (Sephadex LH20; water). The pure fractions were combined and freeze dried to give the sodium 4-sulfophenylthiourea terminated PAMAM 4.0 dendrimer as a fluffy white tlid (370mg). 'H nmr (D20): 8 2.28; 2.52; 2.69; 3.15; 3.27;

IU

WO 95/34595 PCT/AU95/00350 18 3.60; 7.32 J=9Hz); 7.72 J=9Hz). 3 C nmr (D 2 0) 8 36.9; 41.1; 43.1; 48.3; 53.6; 55.8; 129.0; 131.1; 144.4; 178.5; 179.1; 184.4.

The corresponding PAMAM 1.0 and PAMAM 2.0, PAMAM 3.0 and PAMAM 5.0 (Compound No. 2) dendrimers terminated with 3, 6, 12 and 48 sodium 4-sulfophenylthiourea groups respectively were similarly prepared.

B. PAMAM 4.0 (EDA) (Compound No. 3) Solid sodium 4-sulfophenylisothiocyanate monohydrate (130mg; 0.5mmol) was added to a solution of PAMAM 4.0 (EDA) (69mg; 0.Olmmol) in water (4ml) and the resulting solution heated under nitrogen at 530 for two hours and then cooled.

The solution was concentrated and the solid residue purified by gel filtration (Sephadex LH20; water). The pure fractions were combined and freeze dried to give PAMAM 4.0 terminated with 32 sodium 4-sulfophenylthiourea groups as a fluffy white solid (136mg). 'H nmr (D 2 0) 8 2.30; 2.50; 2.70; 3.18; 3.62; 7.35 J=9Hz); 7.72 J=9Hz). 3 C nmr (D 2 0) 8 36.8; 41.0; 43.1; 48.4; 53.6; 55.7; 128.9; 131.0; 144.3; 178.5; 179.0; 184.5.

C. BHAlyslyslys41ys 8 lysl 6 (Compound No. 4) Trifluoroacetic acid (4ml) was added to a suspension of BHAlyslys 2 lys 4 lysDBLi, (0.73g; 0.lmmol).in dry dichloromethane (4ml) under nitrogen. A vigorous evolution of gas was observed for a short time and the resulting solution was stirred at room temperature for two hours and then concentrated. The residual syrup was dissolved in water (5ml), the solution passed through a column of Amberlite IRA-401(OH) and the filtrate concentrated to give BHAlyslys 2 lys 4 lysslys 6 as a viscous oil (0.49g). The oil was redissolved in water and N,N-dimethyl-N-allylamine buffer (pH 9.5; 3ml) added. Solid sodium 4-sulfophenylisothiocyanate monohydrate (1.30g; 5.1mmol) was then added and the resulting solution heated under nitrogen at 530 for two hours and then cooled.

The solution was concentrated and the brownish solid residue purified by gel filtration (Sephadex LH20; water). The pure fractions were combined, passed through a column of Amberlite IR 120(Na) and freeze dried to give the sodium WO 95/34595 PCT/AU95/00350 19- 4-sulfophenylthiourea terminated BHAlysylys2 4 lys4lysy 6 dendrimer as a fluffy white solid (374mg). 'H nmr (D 2 0) 5 1.40; 1.72; 3.08; 3.42; 4.24; 4.60; 7.30; 7.40 J=9Hz); 7.78 J=9Hz). 3 C nmr (DO 2 8 27.3; 32.5; 35.9; 43.7; 48.9; 58.6; 63.3; 128.8; 131.0; 143.7; 144.7; 145.1; 177.7; 178.1; 183.8; 185.2.

The corresponding BHAlyslys21ys41yss, BHAlyslys 2 lys41ysglyst 6 lys 32 (Compound No. and BHAlys 2 lys2 4 1ySglys 16 lys 3 2 ys6 (Compound No. 6) dendrimers terminated with 16, 64, and 128 sodium 4-sulfophenylthiourea groups respectively were similarly prepared.

EXAMPLE 6 Preparation of sodium 3,6-disulfonapthylthiourea terminated dendrimers A. PAMAM 4.0 (Compound No. 9) Solid sodium 3,6-disulfonapthylisothiocyanate (160mg; 0.41mmol) was added to a solution of PAMAM 4.0 (51mg; 0.Olmmol) in water (3ml) and the resulting solution heated under nitrogen at 530 for two hours and the The solution was concentrated and the brown solid residue purified by gel EIraun (Sephadex water). The pure fractions were combined and concentrated to give the sodium 3,6-disulfonapthylthiourea terminated PAMAM 4.0 dendrimer as a brownish solid (73mg). 'H nmr (DO) 5 2.30; 2.60; 2.74; 3.20; 3.57; 7.75; 7.86; 8.28. 3 C nmr (D 2 0) 5 35.0; 39.9; 43.1; 48.1; 53.8; 56.1; 128.4; 128.6; 129.3; 131.0; 131.3; 136.0; 136.8; 138.2; 145.5; 146.0; 177.2; 177.8; 185.5.

The corresponding PAMAM 2.0 dendrimer terminated with sodium 3,6disulfonapthylthiourea groups was similarly prepared.

B. PAMAM 4.0 (EDA) (Compound No. 11) Solid sodium 3,6-disulfonapthylisothiocyanate (220mg; 0.57mmol) was added to a solution of PAMAM 4.0 (EDA) (74mg; 0.01mmol) in water (4ml) and the resulting solution heated under nitrogen at 530 for two hours and then cooled. The solution was concentrated and the brownish solid residue purified by gel filtration (Sephadex LH20; water). The pure fractions were combined and concentrated to WO 95/34595 PCT/AU95/00350 20 give PAMAM 4.0 terminated with 32 sodium 3,6-disulfonapthylthiourea groups as a tan solid (148mg). 'H nmr (D 2 0) 2.30; 2.80; 3.20; 3.54; 7.74; 7.85; 8.25.

"C nmr (D 2 0) 8 36.0; 40.8; 43.1; 48.3; 53.6; 55.9; 128.5; 129.4; 131.0; 131.3; 136.0; 136.8; 138.3; 145.5; 146.0; 178.2; 185.6.

C. BHAlyslylslysslys, 6 (Compound No. 12) Trifluoroacetic acid (2ml) was added to a suspension of BHAlyslys 2 lys 4 lysDBL, 6 (73mg; 0.Olmmol) in dry dichloromethane (2ml) under nitrogen. A vigorous evolution of gas was observed for a short time and the resulting solution was stirred at room temperature for two hours and then concentrated. The residual syrup was dissolved in water (5ml), the solution passed through a column of Amberlite IRA-401(OH) and the filtrate concentrated to give BHAlyslys 2 lys41ys 8 lys 6 as a viscous oil. The oil was redissolved in water and N,N-dimethyl-N-allylamine buffer (pH 9.5; 3ml) added. Solid sodium 3,6disulfonapthylisothiocyanate (234mg; 0.60mmol) was then added and the resulting solution heated under nitrogen at 53* for two hours and then cooled. The solution was concentrated and the brownish solid residue purified by gel filtration (Sephadex LH20; water). The pure fractions were combined, passed through a column of Amberlite IR 120(Na) and freeze dried to give BHAlyslyslys 4 1ys 8 lys1 terminated with 32 sodium 3,6-disulfonapthylthiourea groups as a fluffy off-white solid (119mg). 'H nmr (D 2 0) 8 1.0-2.0; 3.18; 3.43; 4.31; 7.22; 7.80; 7.89; 8.25.

"C nmr (D 2 0) 8 27.2; 32.4; 35.3; 43.7; 49.0; 58.5; 63.6; 128.4; 129.1; 131.4; 136.1; 136.6; 138.6; 139.0; 145.1; 145.6; 178.4; 184.8; 186.7.

EXAMPLE 7 Preparation of sodium 4-sulfonapthylthiourea terminated dendrimers PAMAM 4.0 (Compound No. 8) Solid sodium 4-sulfonapthylisothiocyanate (180mg; 0.5mmol) was added to a solution of PAMAM 4.0 (51mg; 0.Olmmol) in water (5ml) and the mixture heated under nitrogen at 530 for two hours and then cooled. The water was distilled under reduced pressure from the resulting suspension and the off white solid residue purified by gel filtration (Sephadex LH20; water). The pure fractions were

I,

WO 95/34595 PCT/AU95/00350 -21 combined and freeze dried to give the sodium 4-sulfonapthylthiourea terminated PAMAM 4.0 dendrimer as a fluffy white solid (60mg). 'H nmr (D20) 8 2.20; 2.60; 3.14; 3.48; 7.23; 7.47; 7.56; 7.77; 7.93 J=6Hz); 8.56 J=6Hz)."C nmr

(D

2 0) 5 35.8; 40.5; 43.1; 48.4; 53.6; 55.9; 127.6; 128.6; 130.3; 131.9; 132.5; 133.5; 134.7; 140.5; 142.7; 177.8; 178.0; 185.4.

EXAMPLE 8 Preparation of sodium 3,5-disulfophenylthiourea terminated dendrimers PAMAM 4.0 (Compound No. 7) Solid sodium 3,5-disulfophenylisothiocyanate (110mg; 0.32mmool) was added to a solution of PAMAM 4.0 (63mg; 0.012mmol) in water (3ml) and the resulting solution heated under nitrogen at 530 for two hours and then cooled. The solution was concentrated and the brownish solid residue purified by gel filtration (Sephadex G25; water). The pure fractions were combined and concentrated to give PAMAM 4.0 terminated with 24 sodium 3,5-disulfophenylthiourea groups as an off-white solid (110mg). 1H nmr (D 2 0) 6 2.53; 3.08; 3.36; 3.66; 7.90; 7.95. 13C nmr (D 2 0) 6 34.8; 41.0; 43.1; 48.0; 53.7; 56.2; 124.1; 128.6; 143.5; 148.8; 177.6; 185.0.

EXAMPLE 9 Preparation of sodium 3, 6, 8-trisulfonaphthylthiourea terminated dendrimers PAMAM 4.0 (Compound No. Solid sodium 3, 6, 8-trisulfonaphthylisothiocyanate (250mg; 0.5mmol) was added to a solution of PAMAM 4.0 (51mg; 0.Olmmol) and N,N-dimethyl-N-allylamine buffer (pH 9.5; lml) in water (2ml) and the mixture heated under nitrogen at 530 for two hours and then cooled. The mixture was concentrated under reduced pressure to give an orange solid. The residual solid was dissolved in water (2ml) and passed through a short column of Amberlite IR-120(Na). The filtrate was then concentrated and the residue purified by gel filtration (Sephadex LH20; water).

The pure fractions were combined and freeze dried to give the sodium 3, 6, 8i I- I~ WO 95/34595 PCT/AU95/00350 22 trisulfonaphthylthiourea terminated PAMAM 4.0 dendrimer as an off-white solid (102mg). IH nmr (D 2 0) 6 2.65; 3.02; 3.30; 3.66- 8.05; 8.42; 8.59; 8.67. 13C nmr (D 2 0) 6 33.2; 38.7; 43.2; 43.7; 47.8; 54.0; 54.3; 56.7; 131.0; 131.3; 131.9; 135.9; 138.0; 139.6; 143.8; 144.1; 145.6; 176.2; 176.5; 186.0.

EXAMPLE 10 Preparation of sodium 4-(sulfomethyl)benzamide terminated dendrimers PAMAM 4.0 (Compound No. 13) Solid 4-nitrophenyl 4-(chloromethyl)benzoate (200mg; 0.68mmol) was added to a stirred solution of PAMAM 4.0 (70mg; 0.014mmol) in dry DMSO (4ml) and the resulting yellow solution stirred at room temperature foi' two hours. The solution was then concentrated (10 4mmHg; 40 and the residue extracted with a mixture of water and dichloromethane The remaining solid material was dissolved in DMSO (5ml) and a solution of sodium sulfite (130mg; Immol) in water (3ml) added. The slightly cloudy mixture that resulted was left to stand for four days, after which time the addition of more water (2ml) resulted in the formation of a clear homogeneous yellow solution. The solution was then concentrated, first at 25mmHg and 400 then at 10 mmHg and 500 to give the crude product. The crude product was purified by gel filtration (Sephadex water) to give PAMAM 4.0 terminated with 24 sodium 4-(sulfomethyl)benzamide groups (24mg). 1H nmr (20) 6 2.25; 2.66; 3.08; 3.20; 3.33; 3.38; 4.01; 7.40 13 (br 7.62 (br C nmr (D20) 6 36.7; 40.9; 43.0; 43.6; 53.5; 55.5; 61.0; 131.6; 135.0; 137.2; 140.4; 174.5; 178.6; 179.2.

EXAMPLE 11 Preparation of 4-sulfobenzamide terminated dendrimers PAMAM 4.0 (EDA) Solid potassium N-hydroxysuccinimidyl 4-sulfobenzoate (100mg; 0.3mmol) was added to a solution of PAMAM 4.0 (EDA) (35mg; 0.005mmol) in 0.1M pH borate buffer (5ml) and the solution stirred at room temperature for two hours.

The resulting milky solution at this stage had a pH of 4.5. 1M Sodium carbonate I i IP- LI-~ i qlC WO 95/34595 PCT/AU95/00350 -23 solution (Iml) was then added to give a clear solution which was concentrated to give the crude product as a white solid. The crude product was purified by gel filtration (Sephadex G25; water) to give PAMAM 4.0 (EDA) terminated with 32 sodium 4-sulfobenzamide groups (47mg). 1H nmr (D20) 6 2.25; 2.42; 2.63; 3.05; 3.18; 3.31; 3.38; 7.72 J=8Hz); 7.78 J=8Hz). 13C nmr (D 2 6 36.0; 40.4; 43.0; 43.7; 53.7; 55.8; 130.2; 132.2; 140.4; 150.1; 173.6; 178.0; 178.5.

EXAMPLE 12 Preparation of Sodium N-(4-sulfophenyl)propanamide terminated dendrimers PAMAM 4.0 (EDA) Solid sodium N-(4-sulfophenyl)acrylamide (250mg; Immol) and solid sodium carbonate (106mg; Immol) were added successively to a stirred solution of PAMAM 4.0 (EDA) (78mg; 0.01 1mmol) in water (4ml). The resulting solution was stirred under nitrogen for four days and then freeze dried to give a fluffy white solid. The crude product was purified by gel filtration (Sephadex water to give PAMAM 4.0 (EDA) terminated with 64 sodium N-(4- 13 sulfophenyl)propanamide groups (206mg). 1C nmr showed a faint trace of what was taken to be mono alkylated terminal amino groups. H nmr (D20): 2.10; 2.48; 2.58; 2.79; 3.20; 7.42 J=7Hz); 7.65 J=7Hz). 13C nmr (D20) 36.5; 37.9; 41.1; 53.4; 55.6; 124.8; 130.9; 143.0; 144.2; 177.4; 178.5.

EXAMPLE 13 Preparation of Sodium 4-sulfophenylurea terminated dendrimers PAMAM 4.0 (EDA) A solution of sodium sulfanilic acid (195mg; Immol) in dry DMSO (3ml) was added dropwise to a solution ofN,N'- disuccinimidyl carbonate (530mg; 2mmol) in dry DMSO (4ml) and the resulting brownish solution stirred at room temperature for 20 hours. A solution of PAMAM 4.0 (EDA) (75mg; 0.011mmol) in dry DMSO (Iml) added and the solution stirred for a further 18 hours. The solution was then concentrated under high vacuum (10 mmHg; 35 to give a WO 95/34595 PCT/AU95/00350 -24yellowish semi solid. The crude product was dissolved in DMSO (4ml) and the solution added to 200ml of well stirred ethyl acetate. The precipitated white solid was collected by filtration and washed with ethyl acetate (2X) and ether (2X), then dried to give a white powder (275mg). This material was further purified by gel filtration (Sephadex LH20; water) to give PAMAM 4.0 (EDA) terminated with 32 sodium 4-sulfophenylurea groups (106mg). 1H nmr (D20): 6 2.31; 2.55; 2.75; 3.19; 7.32 J=9Hz); 7.63 J=9Hz). 13C nmr (D 2 6 36.3; 40.7; 43.3; 43.8; 53.7; 55.7; 123.3; 130.9; 140.9; 146.0; 161.4; 178.2; 178.6.

EXAMPLE 14 Preparation ofN,N,N-trimethylglycinamide chloride terminated dendrimers.

BHAlyslys 2 lys 4 1ys 8 lys (Compound No. Trifluoroacetic acid (4ml) was added to a suspension of BHAlyslys 2 lys 4 lysDBLi6 (220mg; 30pmol) in dry dichloromethane (2ml) and the resulting solution stirred at room temperature under nitrogen for two hours and then concentrated. The residue was dissolved in dry DMSO (5ml) and the pH adjusted to 8.5 with triethylamine. Solid 4-nitrophenyl N,N,N-trimethylglycinate chloride (0.50g; 1.8mmol) was then added and the mixture stirred overnight at room temperature.

The cloudy solution was then concentrated (500/13" 5 mmHg) and the residue partitioned between water and dichloromethane. The aqueous layer was separated, washed with dichloromethane (3X) and ethyl acetate, and then concentrated to give an oil (1.128g). The crude product was purified by gel filtration (Sephadex water) to give the N,N,N-trimethylglycinamide terminated BHAlyslys 2 lys 4 lyslys, 6 dendrimer (116mg). "C nmr (D 2 8 25.5, 30.5, 30.8, 33.4, 42.1, 56.5, 57.1, 67.5, 68.1, 166.7, 167.0, 167.1, 176.0, 176.2.

EXAMPLE 15 Preparation of 4-Trimethylammoniumbenzamide terminated dendrimers PAMAM 4.0 (Compound No. 16) i WO 95/34595 PCT/AU95/00350 1,1'-Carbonyldiimidazole (85mg; 0.52mmol) was added to a solution of 4trimethylammoniumbenzoic acid iodide (154mg; 0.5mmol) in dry DMF (4ml) and the mixture stirred at room temperature under argon for two hours. During this time a white solid separated from the solution. A solution of PAMAM 4.0 (58mg; 0.01 immol) in dry DMF (2ml) was then added and the mixture stirred overnight at room temperature. After this time most of the precipitate had dissolved and a ninhydrin test of the solution was negative. The mixture was concentrated mmHg; 300) to give a white solid residue. The crude product was purified by gel filtration (Sephadex LH20; 10% AcOH) to give PAMAM 4.0 terminated with 24 4-trimethylammoniumbenzamide groups as the acetic acid salt (89mg). 1H nmr

(D

2 0) 6 1.96; 2.65-2.85; 3.25-3.55; 3.64; 7.92. 13C nmr (D 2 0) 625.8; 33.1; 33.5; 38.7; 43.1; 43.5; 53.5; 54.1; 56.4; 61.2; 124.8; 133.6; 139.9; 153.2; 173.2; 176.3; 176.8; 182.6.

The corresponding PAMAM 2.0 dendrimer terminated with 6 4trimethylammonium benzamide groups was similarly prepared.

EXAMPLE 16 Preparation of 4-(Trimethylammoniummethyl)benzamide terminated dendrimers PAMAM 4.0 (Compound No. 17) Solid 4-nitrophenyl 4-(chloromethyl)benzoate (150mg; 0.5nmmol) was added to a stirred solution of PAMAM 4.0 (52mg; 0.01mmol) in dry DMSO (3ml). The resulting yellow solution was stirred at room temperature for 20 hours, when a ninhydrin test was negative (pH ca.8.5). The solution was then concentrated 40 and the residue shaken with a mixture of water and dichloromethane The insoluble gel-like material was collected by filtration, washed with water (2X) and dichloromethane and then air dried. The crude 4-(chloromethyl)benzamide terminated dendrimer was dissolved in 25% aq.

trimethylamine (20ml) and the yellow solution left to stand overnight. The solution was then concentrated, the residue dissolved in water (5ml) and the solution passed through a column of Amberlite IRA-401 The colourless -Lsrr~BB ~PLls WO 95/34595 PCr/AU95/00350 26 filtrate was concentrated to give a viscous oil which was purified by gel filtration (Sephadex G10; 10% AcOH) to give PAMAM 4.0 terminated with 24 4- (trixnethylammnoniummethyl)benaxnide groups (90mg). IH nmr (D)20) J 1.88; 13 2.65-2.80; 2.98; 3.10-3.60; 7.52 (br d, J=9Hz); 7.72 (br d, J=9Hz). C nmr (D20) 6J 26.6; 33.4; 38.8; 43.2; 43.5; 53.6; 53.6; 54.1; 56.8; 62.8; 73.0;i 132.1; 135.3; 137.5; 140.0; 176.4; 176.9; 183.6.

EXAMPLE. 17 Preparation of N-(2-Acetoxyethyl)-,N,N- (dimethylammonium)methylcarboxamide terminated in~' dendrimers PAMAM Solid 1,l'-carbonyldiinuidazole (85mg; 0.52mmol) was added to a solution of N- (2-acetoxyethyl)-N-(carboxymethyl)-N,N-dimethylammonium bromide (135mg; 0.5mmol) in dry DMF (3m1) and the resulting solution stirred under nitrogen for two hours. A solution of PAMAM 4.0 (60mg; 0.Ol2nimol) in DMF (2rnl) was then added, which caused the immediate formation of a flocculant precipitate which slowly redissolved. The mixture was stirred for two days and then concentrated (10 -4mmHg; 40 0) to give a viscous oil. The crude product was purified by gel filtration (Sephadex GIO; 10% AcOH) to give PAMAM te rrm in at ed with Z24 N '2 -A c e tox y ethy I-N ,N (dimethylammoniun )methylcarboxaniide groups (64mg). IH nmr (D)20):16 1.93; 2.05; 2.70; 3.10-3.60; 3.28; 3.93 4.14; 4.48 13C ninr (D0 6 24.6; 26.2; 33.2; 38.7; 42.8; 42.9; 53.9; 57.4; 62.6; 67.3; 67.5; 158.9; 176.4; 176.8; 177.3; 183.2.

EXAMPLE 18 Preparation of Guanidino terminated dendrimers PAMAM 4.0 (Compound No. 18) 3V A solution of PAMAM 4.0 (63mg; 0.0 l2mmol) and methylthiopseudourea sulfate (1 70mg; 0.61lmmol) ir. water (5mb) (pH 10.5) was heated under nitrogen at, 800 for two hours. The solution was then concentrated and the residue purified by gel WO 95/34595 PCr/AU95/00350 -27filtration (Sephadex G10; 10% AcOH) to give PAMAM 4.0 terminated with 24 guanidino groups as the acetate salt (107mg). H nmr (D 2 2.00; 2.80 (brt); 3.09 (br 3.32; 3.45 (br 3.60 (br 13C nmr (D 2 0) 6 25.2; 33.2; 33.4; 38.7; 41.2; 42.6; 43.4; 44.7; 53.5; 54.0; 56.3; 176.5; 176.7; 176.9; 181.6.

The corresponding PAMAM 2.0 dendrimer terminated with 6 guanidino groups was similarly prepared.

EXAMPLE19 P repa rati o n o f 1,4, 8 11tetraazacyclotetradecane] methyl)benzamide terminated dendrimers PAMAM 4.0 (Compound No. 19) A solution of 1-(4-carboxyphenyl)methyl-l,4,8,11-tetraazacyclotetradecane tetra hydrochloride (120mg; 0.25mmol), N-hydroxysuccinimide (60mg; 0.52mmol) and 1-(3-dimethylaminopropy)-hy)-3-ethylcarbodiimide hydrochloride (250mg; 1.3mmol) in pH 7 phosphate buffer (10ml) was allowed to stand a room temperature for one hour and then a solution of PAMAM 4.0 (32mg; 0.006mmol) in pH 7 phosphate buffer (10mi) added. The mixture was allowed to stand for two days and then concentrated. The residue was purified by gel filtration (Sephadex LH20; AcOH) to give PAMAM 4.0 terminated with ca. 12 4-([1,4,8,11- 1 13 tetraazacyclotetradecane]methyl)benzamide groups as determined by H and C nmr (80mg). The product was then dissolved in water and passed through a column of Amberlite IRA-401 (Cl) resin and then concentrated. The residue was dissolved in water (Iml), concentrated HCI (iml) added, and the solution diluted with ethanol (30ml) to precipitate a white solid. The solid was collected by 1 13 filtration (68mg). Once again H and C nmr showed ca. 50% functionalisation of the terminal amino groups. H nmr (D 2 0) 6 2.17; 2.36; 2.50; 2.78; 2.85; 3.25; 3.40; 3.50; 3.60; 3.62; 4.49; 7.63 (br 7.78 (br 3C nmr (D 2 0) 6 22.7; 23.1; 33.2; 38.8; 39.9; 40.2; 40.3; 41.0; 41.2; 42.0; 42.9; 43.2; 43.6;45.5; 46.1; 49.1; 52.2; 53.9; 54.3; 56.6; 62.7; 132.5; 135.7; 137.1; 139.7; 174.3; 176.2; 176.3; 176.7; 177.0; 178.2; 178.5.

WO 95/34595 PCT/AU95/00350 -28 EXAMPLE 20 Preparation of 4-Carboxy-3-hydroxybenzylamine terminated dendrimers PAMAM 4.0 (EDA) Sodium cyanoborohydride (32mg; 0.5mmol) was added to a mixture of PAMAM (EDA) (69mg; 0.Olmmol), 4-formyl-2-hydroxybenzoic acid (83mg; and sodium hydrogen carbonate (42mg; 0.5mmol) in water (4ml). The inhomogeneous orange mixture was stirred for four hours at room temperature, during which time it became homogeneous. The orange solution was then concentrated and the residue purified by gel filtration (Sephadex LH20; water) to give PAMAM 4.0 (EDA) terminated with ca. 32 4-carboxy-3-hydroxybenzylamine groups (91mg). 1H andl3C nmr (D20) shows mostly mono alkylation but with some signs of dialkylation of the terminal amino groups, both spectra show broad peaks. "C nmr (D 2 0):6 37.0; 41.1; 50.9; 53.4; 55.5; 55.8; 61.5; 120.9; 122.2; 122.4; 132.3; 132.7; 135.0; 135.8; 163.5; 163.7; 169.0; 178.6; 179.3.

H nmr (D 2 6 2.20; 2.35; 2.60; 3.15; 3.30; 3.55; 4.25; 6.68; 7.12; 7.55.

EXAMPLE 21 Test for anticoagulant activity Bovine blood was collected from the abattoir, where an animal was bled into a bucket containing sodium citrate at a concentration of 3.5g per litre of fresh blood.

This blood was returned to the laboratory where it was kept in a 37 0 C water bath.

Aliquots of the whole blood were then centrifuged at 3000rpm for 5 minutes to separate the plasma. This was collected and returned to the water bath. Extra plasma was also prepared and stored in liquid nitrogen for later testing.

The procedure actually tests the recalcification time of the citrated blood at 37 0

C.

All glassware was washed, dried and silated with 'Coatasil' before re-washing and drying. Each 12 X 75mm culture tube contained O.lml of plasma, O.lml of saline solution NaC.) followed by O.1ml of 0.025M CaCl, at which time the stop WO 95/34595 PCT/AU95/00350 29 watch was started. Every 15 sec the tube was tilted to one side and clotting was assessed. When a firm clot had formed, the watch was stopped and the time recorded. In the case of testing anti-coagulants 0.1 ml of the test substance replaced the saline. The times for a range of concentrations for the test compounds are recorded in Table 1. Heparin, sodium citrate and test compouond were made up in water as 10mg/ml solutions. These solutions were diluted to give a range of concentrations. The final concentrations in the test tubes are given in the table.

The figures in the table represent average times for, up to ten replicates.

TABLE 1 Time for plasma coagulation at 37"C for following concentrations of anticoagulant Compound Control 0.0003 0.003 0.03 0.33 3.33 s mg/ml mg/ml mg/ml mg/m g/mlmgm Heparin 2.29min 2.30min >30min >30min >30min Sodium citrate 2.29min 2.30min 2.30min Compound No.1 2.11min 2.29min 4.00min Compound No.4 2.08min 1.72min 10.00min* Compound No.15 2.20min 2.34min 7min* 8mln* Compound No.9 2.27min 2.19min 4min 5.40min EXAMPLE 23 The results Test for antiviral activity.

of tests of activity against HIV1, HIV2, CMV and various herpes simplex viruses (HSV) are recorded in Tables 2 to 5, respectively.

WO 95/34595 PCT/U95/00350 (1) (2) (3) (4) (6) (7) 30 TABLE 2 HIV 1 Activity Results DendriTer erminal Group JECso iM JCC5o P Antivral PAMAM 4.0 s 0.0492 >125 >2543 -Nf NH 24 end groups

H

Sq 3 Na PAMAM 5.0 S 0.0096 >11 >1180 -N A NH 48 end groups

H(

SO

3 Na PAMAM 4.0 (EDA) S 0.0142 >17.2 >1212 -N ANH 32 end groups H 0

SO

3 Na BH-IAlyslS5ysl6 32 end groups BHklysS311YS32 64 end groups BHAlys63lys64 128 end groups PAMAM 4.0 24 end groups

S

-N kNH SONa

S

-N 'k NH

SO

3 Na

S

N

NH

ONa

S

-N.NH

H H NaO 3 S SqNa U.ui 4 2c ILuJ.Y I 0.0332 0.0530 0.003 >10 >5 >18.8 >320 >100 >9091

IYYT

SUBSTITUTE SHEET (RULE 26) WO 95/34595 PCTIAU95OO350 31 (8) (9)

(U)

(12) (13) (14) (1) (16) PAMAM 4.0 24 end groups PAMAM 4.0 24 end groups Pa-MAM 4.0 24 end groups PAMAM 4.0 (EDA) 32 end groups BHAysl5lysl6 32 end groups PAMAM 4.0 24 end groups PAMAM 4.0 24 end groups 3HAlys5lysl16 32 end groups PAMAM 4.0 24 end groups

S

-RNH

NaO 3

SO

3

N

S

-NANH ON NaO3S't )SO 3 Na

S

.Nol N SO Na

-CH

NaO 3 S iJi SO 3 Na

S

-ANH

NaO 3 S iJi-j. SO 3 Na 91 SQ N

I

NaO 3 S HNa

H

-N

SQNa HOQC., Mle 3

NCICONH

CONH-

Nft 0.0694 0.0032 0.0041 0.0051 0.0088 0.0088 0.2849 0.0725 1.2559 >20 >125 >14 >12.9 >15 >24 >25 >125 >25 >300 >39000 >3500 >2537 >1700 >2737 >88 >1720 SUBSTITUTE SHEET (RULE 26) I WO 95/34595 WO 9534595PC/AU95/00350 32 (17) (18) (19) PAMAM 4.0 24 end groups PAMAM 4.0 24 end groups PAMAM 4.0 ca. 12 end groups

CONH-

*NMe 3

NH

H~ NBQCNH.

Ko^) 0.2973 0.2739 0.0538 2.3406 3 .498 3 3.2183 SUBSTITUTE SHEET (RULE 26) WO 95/34595 3PC/AU9S/00350 (1) (2) (3) (4) (6) (7) 33- TABLE 3 HIV 2 Activity Results Dendrimer Terminal Group -ECSo gM I MV PAMAM 4.0 S 0.2237 >125 >560 N PNH 24 end groups

H

SO

3 Na PAMAM 5.0 S 0.1490 >11 >76 N kNH 48 end groups

H

SQ

3 Na PAMAM 4.0 (EDA) S 0.2368 >17.2 >73 N ANH 32 end groups H :1 SQ3Na BHADYsS1ys16 s U 108.0417 9 32 end groups 0

SQ

3 Na BHAys311ys32 S 0.2063 >10 >52 64 end groups

SO

3 Na BHWys631YS64 S 10.2227 5 1>24 128 end groups -[1

SQ

3 Na PAMAM 4.0 S 0.24 >18.8 >77 24 end groups H NaO3S S 3 Na SUBSTITUTE SHEET (RULE 26) 1

-I

WO 95/34595 PCr/AU9S/0035O 34 Y- q-- (8) (9) (1) (12) (13) (14) (16) PAMAM 4.0 24 end groups PAMAM 4.0 24 end groups PAMAM 4.0 24 end groups PAMAM 4.0 (EDA) 32 end groups BHAlysl5lysl6 32 end groups PAMAM 4.0 24 end groups PAMAM 4.0 24 end groups BHAlysl5lysl6 32 end groups PAMAM 4.0 24 end groups

S

H

SO Na

S

-N NH

H

NaO 3 b8' SO 3 Na

S

NIA. NH SO 3 Na

H

NaO 3 S 2

S

3 Na

S

-NI NH

H

Nao 3 Sto SO

S

-Nk NH

H

NaO 3 S t b 3 Na

SQ

3 Na aO3S H Me 3 kNG 2

CNH-

CONH-

4NMe 3 0.4913 0.0674 0.0299 0.0234 0.1778 0.1080 6.002 >125 >25 >20 >125 >14 >12.9 >15 >24 >25 >125 >42 >1854 >493 >551 >222 >4 SUBSTITUTE SHEET (RULE 26) WO 95/34595 WO 954595PAU95OO350 35 (17) (18) (19) PAMAM 4.0 24 end groups PAMAM 4.0 24 end groups PAMAM 4.0 ca. 12 end groups

CONH-

.NM6e3

NH

N--

H NF >7.175 >10.817 0.2175 7.175 10.817 2.4245 L I J. SUBSTITUTE SHEET (RULE 26) WO 95134595 PCT/AU95/00350 36 TABLE 4 Activ/ty against Human Cytomegalovirus Cell culture(Davis strain) Compound -EC5 0 (jig/mi) T CC 5 0 (pg/mi) Compound No. 15 1.0 !250 Compound No. 9 0.2-<0.4 z!250 Tests in human embrionic lung cells (HEL).

1C50= Inhibitory concentration to reduce virus plaque by 50 concentration required to reduce 1-JEL cell growth by TABLE Activity of BRI compounds against miscellaneous viruses.

Minimum Inhibitory Concentrationb (pag/ml) Compound Minimum Herpes Herpes Herpes Herpes Cytotoxic simplex simplex simplex Simplex Concentrationa %iirus-1 virus-2 virus-1 viwus-l (pg/mi) (KOS) TK-B32006 TK-VM WI837, Compound !400 70 7 150 No.4 Compound 400 7 20 20 a Required to cause a microscopically detectable alteration of normal morphology.

b Required to reduce virus-induced cytopathogenicity by

Claims (39)

1. (Amended) An antiviral compound comprising a dendrimer having a plurality of terminal groups wherein at least one of said terminal groups has an anionic- or cationic-containing moiety, other than a 2-thiosialic acid moiety, bonded thereto.

2. A compound according to claim 1, wherein said dendrimer, comprises a polyvalent core covalently bonded to at least two dendritic branches, and extends through at least two generations.

3. A compound according to claim 1 or claim 2, wherein said dendrimer is a polyamidoamine dendrimer based on an ammonia core.

4. A compound according to claim 1 or claim 2, wherein said dendrimer is a polyamidoamine dendrimer based on an ethylene diamine core.

A compound according to claim 1 or claim 2, wherein said dendrimer is a polylysine dendrimer based on a benzhydrylamine or other suitable core.

6. A compound according to any of claims 1 to 5 wherein said anionic- or cationic- containing moiety or moieties are bonded to terminal amine, sulfhydryl, hydroxy or other reactive functional terminal groups of said dendrimer by amide or thiourea linkages.

7. (Amended) A compound according to any of claims 1 .to 6 wherein said anionic- containing moiety or moieties are sulfonic acid-containing moieties or carboxylic acid-containing moieties other than 2-thiosialic acid moieties.

8. A compound according to any of claims 1 to 6 wherein said cationic-containing moiety or moieties are trimethylammonium-containing moieties or polyamino- macrocyclic-containing moieties. AMENDED SHEET IPEA/AU I ~Rs WO 95/34595 PCUTAU95/0,35S 38

9. A compound according to any of claims 1 to 6, wherein the moiety or moieties which are bonded to amino or other reactive functional terminal groups of the dendrimer are selected from the following groups: -NH(CH 2 ),S0 3 -(CH 2 )nSO Ar(S0 3 1 1 -CH 2 CH(S0o3)COOH CH(SCH 2 COOH -ArX(CH 2 )nS0 3 X=0, S, NH (CH 2 ),NMe 3 -Ar(NMe 3 )n Ar(CH 2 NMe 3 )n Y.SO3Na 0 2Jk- SO 3 Na 0 /k/N)(V*SONa 0 0 S $%Na I -OO S HA S0 3 Na S HA Na0 3 SbSONa S HA (K-q S0 3 Na S Na0 3 S HNX NaO 3 S b L0N S0 3 Na CO d%.M 0 0-J e 0 HNA S0 3 Na 0 S0 3 Na S0 3 Na 0~ 0~e 3 EN N' Co SUBSTITUTE SHEET (RULE 26) WO 95/34595 PCT/AU95/00350 39 in which n is zero or a positive integer.

A compound according to any of claims 1 to 6 which is an alkylsulfonic acid terminated dendrimer.

11. A compound according to any of claims 1 to 6 which is a sulfoacetamide terminated dendrimer.

12. A compound according to any of claims I to 6 which is a sulfosuccinamic acid terminated dendrimer.

13. A compound according to any of claims 1 to 6 which is a N-(2-sulfoethyl) succinamide terminated dendrimer.

14. A compound according to any of claims 1 to 6 wherein the moiety bonded to the terminal groups of the dendrimer is an aryl or heteroarylthiourea substituted with one or more sulfonic acid groups.

A compound according to claim 14 which is a 4-sulfophenylthiourea terminated dendrimer.

16. A compound according to claim 14 which is a 3,6-di-sulfonapttylthiourea terminated dendrimer.

17. A compound according to claim 14 which is a 4-sulfonapthylthiourea terminated dendrimer.

18. A compound according to claim 14 which is a terminated dendrimer.

19. A compound according to claim 14 which is a 3,6,8-tri-sulfonapthylthiourea terminated dendrimer.

WO 95/34595 PCT/AU95/00350 A compound according to any of claims 1 to 6 wherein the moiety bonded to the terminal groups of the dendrimer is an aryl or heteroaryl amide substituted with one or more sulfonic acid, sulfoalkyl, sulfoalkoxy, sulfoalkylamino or sulfoalkylthio groups.

21. A compound according to claim 20 which is a 4-(sulfomethyl) benzamide terminated dendrimer.

22. A compound according to claim 20 which is a 4-sulfobenzamide terminated de: rimer.

23. A compound according to any of claims 1 to 6 wherein the moiety bonded to the terminal groups of the dendrimer is an aryl or hetetoaryl alkanamide substituted with one or more sulfonic acid groups.

24. A compound according to claim 23 which is a N-(4-sulfophenyl) propanamide terminated dendrimer.

A compound according to any of claims 1 to 6 wherein the moiety bonded to the terminal groups of the dendrimer is an aryl or heteroaryl urea substituted with one or more sulfonic acid groups.

26. A compound according to claim 25 which is a 4-sulfophenyiurea terminated dendrimer.

27. A compound according to any of claims 1 to 6 wherein the moiety bonded to the terminal groups of the dendrimer is an N.N,N-trimethyl derivative of an amino acid.

28. A compond according to claim 27 which is a NN,N-ti-methylglycinam'de terminated dendrimer. WO 95/34595 PCT/AU95/00350 41

29. A compound according to any of claims 1 to 6 wherein the moiety bonded to the !rminal groups of the dendrimer is an aryl or heteroaryl amide substituted with one or more trialkylamino, trialkylaminoalkyl, trialkylaminoalkyloxy, trialkylaminoalkylamino or trialkylaminoalkylthio groups.

A compound according to claim 29 which is a 4-trimethylaimmonium benzamide terminated dendrimer.

31. A compound according to claim 29 which is a 4-(trimethylammonium methyl)benzamide terminated dendrimer.

32. A compound according to any of claims 1 to 6 which is a N-(2-acetoxyethyl)- N,N-(dimethylammonium)methylcarboxamide terminated dendrimer.

33. A compound according to any of claims 1 to 6 which is a guanidino terminated dendrimer.

34. A compound according to any of claims 1 to 6 wherein the dendrimer is a macrocyclic polyamino group containing one or more macrocyclic rings connected through an alkyl or aryl spacer moiety to the terminal group of the dendrimer.

A compound according to claim 34 which is a 4-([1,4,8,11-tetra- azacyclotetradecane]methyl)benzamide terminated dendrimer.

36. A compound according to any of claims 1 to 6 which is a 4-carboxy-3-hydroxy- benzylamine terminated dendrimer.

37. A pharmaceutical or veterinary composition for prophylactic or therapeutic antiviral treatment of a human or non-human animal, which comprises a compound of any of claims 1 to 36, in association with at least one pharmaceutically or veterinarily acceptable carrier or diluent. 1 I 970723,p:\opc ims,26659/95,42 -42-

38. A method for prophylactic or therapeutic antiviral treatment of a human or non- human animal, which comprises administering to said human or animal or prophylactic- or therapeutic-antiviral-effective amount of a compound of any of claims 1 to 36.

39. A method according to claim 38, wherein said antiviral treatment is treatment of infection by HIV1 or HIV2, Hepatitis B or C, Bovine Viral Diarrhoea Virus, Human Influenza Virus A and B, Rhinovirus, Human Parainfluenza Virus, Respiratory Syncytial Virus (RSV), Varicella Zoster Virus (VSV), Human Cytomegalovirus (CMV), Epstein Barr Virus (EBV), Human Papilloma Virus (HPV), Adenovirus-8, Herpes Simplex Virus (HSV) type 1 and 2, Measles Virus, or Vesicular Stomatitis Virus (VSV). Use of a compound of any of claims 1 to 36, in the manufacture of a medicament for prophylactic or therapeutic antiviral treatment of, a human or non-human animal. Dated this 23rd day of July, 1997. Biomolecular Research Institute By its Patent Attorneys Davies Collison Cave S *o •o