AU6779294A - Enhancing performance capacity by sparing muscle glycogen with medium chain fatty acids - Google Patents
Enhancing performance capacity by sparing muscle glycogen with medium chain fatty acidsInfo
- Publication number
- AU6779294A AU6779294A AU67792/94A AU6779294A AU6779294A AU 6779294 A AU6779294 A AU 6779294A AU 67792/94 A AU67792/94 A AU 67792/94A AU 6779294 A AU6779294 A AU 6779294A AU 6779294 A AU6779294 A AU 6779294A
- Authority
- AU
- Australia
- Prior art keywords
- fatty acids
- medium chain
- chain fatty
- exercise
- triglyceride
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 150000004667 medium chain fatty acids Chemical class 0.000 title claims description 53
- 210000003205 muscle Anatomy 0.000 title claims description 53
- 229920002527 Glycogen Polymers 0.000 title claims description 50
- 229940096919 glycogen Drugs 0.000 title claims description 50
- 230000002708 enhancing effect Effects 0.000 title claims description 7
- 150000001720 carbohydrates Chemical class 0.000 claims description 52
- 238000000034 method Methods 0.000 claims description 29
- 230000037081 physical activity Effects 0.000 claims description 21
- UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 claims description 21
- 230000001965 increasing effect Effects 0.000 claims description 20
- 230000037406 food intake Effects 0.000 claims description 13
- 125000004432 carbon atom Chemical group C* 0.000 claims description 7
- 150000004671 saturated fatty acids Chemical class 0.000 claims description 6
- 235000003441 saturated fatty acids Nutrition 0.000 claims description 5
- 229920006395 saturated elastomer Polymers 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 2
- 239000007787 solid Substances 0.000 claims description 2
- 150000004670 unsaturated fatty acids Chemical class 0.000 claims description 2
- 235000021122 unsaturated fatty acids Nutrition 0.000 claims description 2
- 230000036314 physical performance Effects 0.000 claims 1
- 235000014633 carbohydrates Nutrition 0.000 description 51
- 239000000047 product Substances 0.000 description 44
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 31
- 150000003626 triacylglycerols Chemical class 0.000 description 26
- 150000004668 long chain fatty acids Chemical class 0.000 description 24
- 239000008280 blood Substances 0.000 description 20
- 210000004369 blood Anatomy 0.000 description 20
- 238000012360 testing method Methods 0.000 description 18
- 150000004665 fatty acids Chemical class 0.000 description 16
- 239000000194 fatty acid Substances 0.000 description 15
- 235000014113 dietary fatty acids Nutrition 0.000 description 14
- 229930195729 fatty acid Natural products 0.000 description 14
- 239000003925 fat Substances 0.000 description 13
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 13
- 150000002576 ketones Chemical class 0.000 description 12
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 11
- 239000008103 glucose Substances 0.000 description 11
- GHVNFZFCNZKVNT-UHFFFAOYSA-N decanoic acid Chemical compound CCCCCCCCCC(O)=O GHVNFZFCNZKVNT-UHFFFAOYSA-N 0.000 description 8
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 7
- 230000007423 decrease Effects 0.000 description 7
- 229910052760 oxygen Inorganic materials 0.000 description 7
- 239000001301 oxygen Substances 0.000 description 7
- 102000004877 Insulin Human genes 0.000 description 6
- 108090001061 Insulin Proteins 0.000 description 6
- 229940125396 insulin Drugs 0.000 description 6
- 238000011282 treatment Methods 0.000 description 6
- 101710137760 Malonyl-CoA-acyl carrier protein transacylase, mitochondrial Proteins 0.000 description 5
- 230000008901 benefit Effects 0.000 description 5
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 210000004185 liver Anatomy 0.000 description 5
- 210000003470 mitochondria Anatomy 0.000 description 5
- 239000005632 Capric acid (CAS 334-48-5) Substances 0.000 description 4
- 108010004103 Chylomicrons Proteins 0.000 description 4
- 238000011056 performance test Methods 0.000 description 4
- 230000017531 blood circulation Effects 0.000 description 3
- 235000005911 diet Nutrition 0.000 description 3
- 235000021588 free fatty acids Nutrition 0.000 description 3
- 239000000446 fuel Substances 0.000 description 3
- 235000012054 meals Nutrition 0.000 description 3
- 210000000813 small intestine Anatomy 0.000 description 3
- 125000005457 triglyceride group Chemical group 0.000 description 3
- PHIQHXFUZVPYII-ZCFIWIBFSA-O (R)-carnitinium Chemical compound C[N+](C)(C)C[C@H](O)CC(O)=O PHIQHXFUZVPYII-ZCFIWIBFSA-O 0.000 description 2
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 2
- 210000000577 adipose tissue Anatomy 0.000 description 2
- GZCGUPFRVQAUEE-VANKVMQKSA-N aldehydo-L-glucose Chemical compound OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)C=O GZCGUPFRVQAUEE-VANKVMQKSA-N 0.000 description 2
- 238000001574 biopsy Methods 0.000 description 2
- 238000004159 blood analysis Methods 0.000 description 2
- 210000004204 blood vessel Anatomy 0.000 description 2
- 229960004203 carnitine Drugs 0.000 description 2
- 235000013339 cereals Nutrition 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 230000037213 diet Effects 0.000 description 2
- 229910052739 hydrogen Inorganic materials 0.000 description 2
- 239000001257 hydrogen Substances 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 238000001964 muscle biopsy Methods 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 239000000700 radioactive tracer Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 235000020183 skimmed milk Nutrition 0.000 description 2
- 235000021055 solid food Nutrition 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- LADGBHLMCUINGV-UHFFFAOYSA-N tricaprin Chemical compound CCCCCCCCCC(=O)OCC(OC(=O)CCCCCCCCC)COC(=O)CCCCCCCCC LADGBHLMCUINGV-UHFFFAOYSA-N 0.000 description 2
- 229940093633 tricaprin Drugs 0.000 description 2
- 102000009027 Albumins Human genes 0.000 description 1
- 108010088751 Albumins Proteins 0.000 description 1
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 1
- 108090001030 Lipoproteins Proteins 0.000 description 1
- 102000004895 Lipoproteins Human genes 0.000 description 1
- 239000005913 Maltodextrin Substances 0.000 description 1
- 229920002774 Maltodextrin Polymers 0.000 description 1
- 241000700159 Rattus Species 0.000 description 1
- 241000283984 Rodentia Species 0.000 description 1
- 235000009499 Vanilla fragrans Nutrition 0.000 description 1
- 244000263375 Vanilla tahitensis Species 0.000 description 1
- 235000012036 Vanilla tahitensis Nutrition 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- OGBUMNBNEWYMNJ-UHFFFAOYSA-N batilol Chemical class CCCCCCCCCCCCCCCCCCOCC(O)CO OGBUMNBNEWYMNJ-UHFFFAOYSA-N 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 235000019577 caloric intake Nutrition 0.000 description 1
- 230000025938 carbohydrate utilization Effects 0.000 description 1
- 229940071162 caseinate Drugs 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 235000013353 coffee beverage Nutrition 0.000 description 1
- 230000002860 competitive effect Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 230000000378 dietary effect Effects 0.000 description 1
- 102000038379 digestive enzymes Human genes 0.000 description 1
- 108091007734 digestive enzymes Proteins 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 230000037149 energy metabolism Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 230000002650 habitual effect Effects 0.000 description 1
- 235000012171 hot beverage Nutrition 0.000 description 1
- 125000004435 hydrogen atom Chemical group [H]* 0.000 description 1
- 239000008173 hydrogenated soybean oil Substances 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 210000004324 lymphatic system Anatomy 0.000 description 1
- 229940035034 maltodextrin Drugs 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000000663 muscle cell Anatomy 0.000 description 1
- 210000004165 myocardium Anatomy 0.000 description 1
- 125000004430 oxygen atom Chemical group O* 0.000 description 1
- 230000036284 oxygen consumption Effects 0.000 description 1
- 235000019629 palatability Nutrition 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 230000000241 respiratory effect Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 150000004666 short chain fatty acids Chemical class 0.000 description 1
- 235000021391 short chain fatty acids Nutrition 0.000 description 1
- 210000002027 skeletal muscle Anatomy 0.000 description 1
- 235000021195 test diet Nutrition 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/21—Esters, e.g. nitroglycerine, selenocyanates
- A61K31/215—Esters, e.g. nitroglycerine, selenocyanates of carboxylic acids
- A61K31/22—Esters, e.g. nitroglycerine, selenocyanates of carboxylic acids of acyclic acids, e.g. pravastatin
- A61K31/23—Esters, e.g. nitroglycerine, selenocyanates of carboxylic acids of acyclic acids, e.g. pravastatin of acids having a carboxyl group bound to a chain of seven or more carbon atoms
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/21—Esters, e.g. nitroglycerine, selenocyanates
- A61K31/215—Esters, e.g. nitroglycerine, selenocyanates of carboxylic acids
- A61K31/22—Esters, e.g. nitroglycerine, selenocyanates of carboxylic acids of acyclic acids, e.g. pravastatin
- A61K31/225—Polycarboxylic acids
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Emergency Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Description
ENHANCING PERFORMANCE CAPACITY BY SPARING MUSCLE GLYCOGEN WITH MEDIUM CHAIN FATTY ACIDS FIELD OF THE INVENTION This invention relates to enhancing physical activity performance capacity. In particular, this invention relates to a method of sparing muscle glycogen in a subject by increasing medium chain fatty acids in the subject. More in particular, this invention relates to the use of a triglyceride of medium chain fatty acids to facilitate muscle glycogen sparing during periods of physical activity.
BACKGROUND OF THE INVENTION Triglycerides are the principal form in which fats are eaten and stored in the body. Triglycerides are composed of two different compounds—fatty acids and glycerol. Three fatty acids are attached to each glycerol molecule as follows:
H2COH H2C - OC(O)R
HCOH + 3 fatty acids HC - OC(O)R- + 3 H2O
1
H2COH H2C - OC(O)R" glycerol triglyceride where R, R' and R" are the same or different fatty acid side chains.
The fatty acid side chain comprises a chain of carbon atoms onto which are bonded hydrogen and oxygen atoms. These chains vary in degree of saturation with hydrogen and length. Fatty acids are classified as saturated, monounsaturated or polyunsaturated depending upon the number of hydrogen atoms. For the purposes of this document, short chain fatty acids are defined as containing fewer than six carbons, medium chain fatty acids contain six to twelve carbons, and long chain fatty acids contain fourteen or more carbons.
Most fats are present in food as triglycerides of long chain fatty acids (LCFAs) . After ingestion, these triglycerides are broken down into LCFAs,
monoglycerides, and glycerol which are absorbed into the cell walls of the small intestine. In order to be transported into the body, the LCFAs are then packaged in a fat droplet called a chylomicron. Chylomicrons pass into the lymphatic system and then slowly into the blood stream from which they are subsequently removed by the liver. The chylomicron is then broken down into smaller components called lipoproteins, which are circulated throughout the bloodstream to various tissues, particularly adipose fat storage tissue. LCFAs can be released through further complex processes from the adipose tissue to provide a source of energy to the muscle under certain exercise conditions.
During exercise, triglycerides of medium chain fatty acids (MCFAs) may provide a more readily available source of energy to the muscle because they are metabolized differently than LCFAs.
1. After triglycerides of MCFAs are ingested, MCFAs are released from the triglyceride backbone at higher rates than LCFAs by digestive enzymes in the small intestine.
2. MCFAs, being more soluble in the blood than LCFAs, are transported and circulated differently throughout the body. MCFAs are absorbed directly through the wall of the small intestine. MCFAs are then released directly into the blood stream that flows to the liver. This is unlike LCFAs which must be packaged into chylomicrons for transport in the body.
3. Any fatty acid must enter the mitochondria to be utilized as an energy source. In the case of
LCFAs, a substance called carnitine is required for transport into the mitochondria. Unlike LCFAs, MCFAs can enter the mitochondria without the help of carnitine due to their shorter chain length. MCFAs are converted within the liver to form ketone bodies. These ketone bodies, uniquely derived from MCFAs, may be transported to the muscle to be used as an additional energy source.
Owing to their increased solubility, some MCFAs may bypass the liver and be directly transported to mitochondria located within the muscle as a further source of energy. So, due to these three main differences in metabolism of fatty acids with different chain lengths, MCFAs may provide the muscle with readily available energy.
Physical activity, among other factors, is dependent upon the body's ability to store, deliver and restore energy sources. The diet provides energy mainly from carbohydrates and fats. These energy sources are stored in the body until needed. Protein is not used as an energy source unless the body is in a calorie restricted state. Proteins are the body's building blocks needed for growth and repair of damaged cells such as muscle tissue.
Muscles use energy at a rate proportional to the intensity of physical activity. Energy is produced when the mitochondria contained within muscle cells burn up carbohydrate and fatty acids in the presence of oxygen to make a biochemical compound called ATP. ATP is the substance that actually provides the direct source of energy to the muscles. ATP can also be produced by alternative mechanisms without oxygen, but in this case only carbohydrates and not fats are used.
Although fat provided in the diet can be an important energy source during exercise, most emphasis is placed on the intake and use of carbohydrates. This has resulted from research showing that the use of LCFAs by the muscle during exercise typically decreases as exercise intensity increases. For example, during walking or low intensity exercise, the body uses LCFAs more efficiently than during a fast jog or sprint or high intensity exercise. This inefficiency at higher intensities is primarily due to the inability of the blood to provide LCFAs to the muscle. As exercise
intensity increases, the blood vessels narrow or constrict in the adipose tissue in order to provide adequate blood flow to the working muscles. Blood vessel constriction in the adipose at these high exercise intensities decreases blood flow to the muscle limiting the availability of albumin, a necessary compound required to transport LCFAs in the blood. Therefore, LCFA availability is a limiting factor for the muscle to use fat as an energy source during high intensity exercise.
Exercise intensity is defined as the tempo, speed or resistance of an exercise. Intensity can be increased by working faster—doing more work in a given period of time. It is generally measured by evaluating the metabolic response of an individual challenged by exercise of increasing intensity, therefore increasing oxygen consumption (V02) . A point is reached where, even though the exercise intensity can be increased still further, there is no accompanying increase in oxygen uptake. This is called the maximum oxygen uptake
(V02max) , and is related to size, age, sex, genetic potential and level of habitual activity of the individual. The relative intensity of the oxygen cost of an activity is expressed as a percentage of V02max. The relative exercise intensity reflects the physiological and psychological demands on an individual more than the absolute values for work loads.
Despite this theoretical basis, research groups in the exercise science field did not show any advantage in performance of using triglycerides of MCFAs versus carbohydrate or triglycerides of LCFAs.
In this respect, J. L. Ivy et al., Int. J. Sports Medicine (1980) 1: 15-20 describes that ten well- trained men participated in 60 minutes of exercise on a bicycle ergometer or a motor driven treadmill at 70% of the subjects' V02max (as described earlier) . Subjects randomly consumed a control containing only carbohydrate
(CHO) , 30 grams of triglyceride of MCFAs, otherwise known as medium chain triglyceride (MCT) , mixed into cereal with skim milk, and 30 grams of triglyceride of LCFAs, otherwise known as long chain triglycerides (LCTs) , mixed into cereal with skim milk. All meals were consumed 1 hour before the exercise. Based on the respiratory exchange ratio, the percentage of total energy used by the subject obtained from fat metabolism during all trials was similar. It was concluded that "MCT in combination with carbohydrate is not a viable energy source during an acute endurance exercise."
J. Deco baz et al., Eur. J. Appl. Physiol. (1983) 52: 9-14 followed up the Ivy et al. 1980 study and describes that twelve subjects exercising at 60% of their V02max on a bicycle ergometer for 1 hour found no advantage of ingesting test meals of only MCTs versus carbohydrate (maltodextrin, D.E. = 22). Both test meals provided approximately 238 calories and were presented as a hot, instant decaffeinated coffee drink (300 ml) to aid in the palatability of the test materials. The MCT had previously been prepared as a powdered emulsion with caseinate. The effects of MCT and carbohydrate on energy metabolism and glycogen levels were compared by measuring C02 output (break down products of carbohydrate and fat after their use for energy) , glycogen concentrations via muscle biopsies, and ketone levels (fat by-products) in the blood. The glycogen concentration in the working muscles decreased equally, about 55%, in both groups. They concluded that, with normal carbohydrate stores, the ingestion of MCT did not decrease the contribution of carbohydrate during the exercise protocol.
E. Auclair et al., Eur. J. Appl. Physiol. (1988) 57: 126-131 describes a method to test the premise that fat ingestion could increase endurance by slowing the rate of glycogen depletion. Trained rats ran on a rodent treadmill following infusion of water, glucose, MCTs or LCTs. The intake of MCTs or LCTs did not reduce
glycogen use in liver, heart or skeletal muscle. The conclusion was made that increased fat availability before exercise did not modify glycogen depletion. Finally, D. Massicotte et al., J. Applied Physiology, October (1992) 1334-39 describes subjects participating in 2 hour exercise rides on a bicycle ergometer at 65% of their maximum oxygen uptake (V02max) . Test diets of MCT compared with carbohydrate were ingested 1 hour prior to the exercise ride. 25 grams of MCT in a hot drink flavored with vanilla were ingested 1 hour before the beginning of exercise and compared with the ingestion of a 6% glucose solution. Ingestion of MCT or glucose did not contribute to the reduction of endogenous carbohydrate utilization. This study by Massicotte et al. is unique in contrast to the previously described studies in that glucose and MCTs were labeled with a 13C tracer to assess more accurately their use for energy throughout the exercise bout. Subjects break down the labeled MCT and glucose as 13C02 which is easily measured in the expired breath. The contribution of MCT and carbohydrate to the total energy expenditure as measured with a 13C tracer was not statistically significantly different.
These exercise studies have not found MCTs as a unique energy source under the conditions tested.
As previously stated, MCFAs are metabolized differently than LCFAs. MCFAs may provide a readily available source of energy to the muscle when blood flow is constricted during a high intensity exercise. The discovery that this preferential use of MCFAs by the muscle would decrease the muscle's reliance on its major carbohydrate store, glycogen, would be a substantial advance in the technology of enhancing physical activity performance capacity. The amount of glycogen is limited and the depletion of this fuel is a limitation on duration and intensity of exercise.
OBJECTS OF THE INVENTION
It is thus an object of the invention to provide a means for enhancing physical activity performance capacity. It is another object of this invention to provide a method of sparing muscle glycogen in a subject during periods of high intensity physical activity.
These and other objects of the invention will be readily apparent from the following description and claims.
SUMMARY OF THE INVENTION It has been found for the first time that MCFAs can be made available to the muscle and utilized as an additional fuel source to muscle glycogen and blood glucose at high exercise intensities.
It has also been found for the first time that providing medium chain fatty acids to the body reduces the rate of muscle glycogen utilization under certain exercise conditions. The maintaining of muscle glycogen levels tends to: (1) increase the exercise time before muscle exhaustion is manifested, (2) decrease the time required for glycogen to be restored to capacity prior to the next physical activity, and (3) increase interval training capacity—train more in a given period of time. It has further been found that consumption of a product containing triglycerides of MCFAs provided a benefit in the sprint phase at the end of an endurance activity. Specifically, in sprints at greater than 70% of V02max at the end of an extended exercise bout at 50% of V02max, performance capacity was enhanced, even though a product containing triglycerides of MCFAs was consumed prior to the low intensity exercise indicating that the MCFAs were held in reserve for the sprint.
Under certain test conditions, there was a greater glycogen sparing effect (or lowering of the glycogen utilization rates) for subjects who consumed a product containing a mixture of triglycerides of MCFAs
and carbohydrate compared to other products containing triglycerides of LCFAs and carbohydrate or carbohydrate alone. The glycogen utilization rate (amount of muscle carbohydrate used per minute during exercise) for each subject consuming a product containing triglycerides of MCFAs and carbohydrate was significantly reduced in comparison to subjects who consumed products containing either carbohydrate alone or carbohydrate and triglycerides of LCFAs at the same caloric intake. This indicates that subjects who consume triglycerides of MCFAs can use less muscle carbohydrate during physical activity levels which primarily rely on muscle glycogen as a carbohydrate source.
Nevertheless, how effective these potential benefits are depends upon the duration and intensity of the exercise bout.
In one aspect, the present invention is a method of providing a preferential fuel to enhance performance, particularly a method of sparing muscle glycogen in a subject by increasing medium chain fatty acid for undergoing physical activity at an intensity of greater than 70% of V02max. Advantageously, the concentration of medium chain fatty acid is increased by administering a triglyceride of a least one medium chain fatty acid, particularly by administering a triglyceride of three medium chain fatty acids, to the subject. The medium chain fatty acids are saturated or unsaturated fatty acids having 6 to 12 carbon atoms. Advantageously, the medium chain fatty acids are saturated fatty acids having 6 to 12 carbon atoms, particularly a saturated fatty acid having 10 carbon atoms.
The triglyceride is administered in an amount effective for sparing muscle glycogen, preferably 1 to 500 grams, more preferably 4 to 50 grams. Advantageously, the physical exercise intensity is at greater than 70% of V02max, more preferably at least
75% of V02max, still more preferably at least 85% of V02max.
The medium chain triglyceride may be administered in a solid or liquid form. Preferably, the triglyceride is administered by ingestion. The triglyceride may be administered together with a carbohydrate and/or other ingredients.
The concentration of medium chain fatty acid is increased before the subject undergoes the physical activity or while the subject undergoes the physical activity.
The invention is also a method of sparing muscle glycogen in a subject by increasing medium chain fatty acid in the subject for undergoing physical activity at a level of muscle activity at which glycogen would be metabolized as a source of energy, particularly a method of enhancing physical activity performance capacity of a subject by administering to the subject a triglyceride of medium chain fatty acids for undergoing physical activity at an intensity greater than 70% of V02max.
DETAILED DESCRIPTION OF THE INVENTION
The present invention is further described and illustrated in the following examples. Further objects of this invention, together with additional features contributing thereto and advantages accruing therefrom, will be apparent from the following examples of the invention. It will be appreciated that variations and modifications to the products and methods can be made by the skilled person without departing from the spirit or scope of the invention as defined in the appended claims.
EXAMPLE 1
After consumption of a solid food bar (the MCT product) containing 16% by weight of triglycerides of capric acid (tricaprin) and 73% by weight carbohydrate, or an otherwise identical solid food bar (the LCT product) wherein the 16% by weight tricaprin is replaced by 16% by weight of partially hydrogenated soybean oil, glycogen use rates were compared during similar exercise protocols at 85% of V02max for 30 minutes. EXERCISE PROTOCOL
Ten (n=10) highly trained, male competitive cyclists, classified as national caliber cyclists, participated in this study. All subjects performed all exercise bouts in a double-blind random order. The cyclists completed three 30 minute rides on separate days during which the two different dietary treatments were randomly assigned. Workloads, based on economy tests prior to participation in the study, corresponded to intensities of 85% of maximum oxygen uptake (85% V02max) . The exercise bout was of a continuous nature with no rest intervals during the 30 minute exercise regimen.
Subjects were tested in the morning in the fasted state. One hour prior to the start of the 30 minute exercise bout, subjects consumed 100 grams of the MCT product or 100 grams of the LCT product. BLOOD ANALYSIS
Blood analysis was done before, at 15 minutes and after the 30 minute ride. Blood samples were analyzed for (1) glucose, (2) insulin, (3) free fatty acids, (4) triglycerides, (5) glycerol, and (6) ketone bodies. MUSCLE ANALYSIS
A muscle biopsy of the vastuε lateralis was taken prior to the exercise bout and after the 30 minute ride. Muscle samples were analyzed for glycogen.
MUSCLE GLYCOGEN USE
The amount of muscle carbohydrate used per minute during exercise was significantly less for subjects consuming the MCT product versus the LCT product under identical exercise protocols.
The results were as follows:
Glycogen Utilization Rate (mmol/kg/min)
MCT product 0.46
LCT product 1.01
BLOOD PARAMETERS
There was no significant difference between blood values of fatty acids, ketones, glucose or insulin at the end of the exercise bouts for all treatment groups. This indicates that fatty acids and ketones provided by the MCT product were used immediately as a source of energy.
Glycerol, the backbone of the triglycerides of capric acid and of LCFAs, was present at higher levels for the subjects consuming the MCT product versus the LCT product. This indicates that MCFAs were being removed from the glycerol backbone more readily than LCFAs during the exercise protocol.
EXAMPLE 2
After consumption of 100 grams of the MCT product of Example 1 or the caloric equivalent of carbohydrate only, glycogen use rates were compared during similar exercise protocols at 85% of V02max for 30 minutes.
The test protocol was the same as in Example 1. MUSCLE GLYCOGEN USE
The amount of muscle carbohydrate used per minute during exercise was significantly less for subjects consuming the MCT product versus the subjects
given carbohydrate alone under identical exercise protocols.
The results were as follows:
Glycogen Utilization Rate (mmol/kg/min)
MCT product 0.46
Carbohydrate 1.09
BLOOD PARAMETERS
There was no significant difference between blood values of fatty acids, ketones, glucose or insulin at the end of the exercise bouts for all treatment groups. This indicates that fatty acids and ketones provided by the MCT product were used immediately as a source of energy.
Glycerol, the backbone of the triglycerides of capric acid, was present at high levels for subjects consuming the MCT product indicating that MCFAs were being removed from the triglyceride backbone during the exercise protocol.
EXAMPLE 3
After consumption of 100 grams of the MCT product of Example 1 or the caloric equivalent of carbohydrate only, glycogen use rates were compared during similar exercise protocols at 100% of V02max for 5 minutes.
The test protocol was similar to Example 1, however five subjects were tested. MUSCLE GLYCOGEN USE
The amount of muscle carbohydrate used per minute during exercise was significantly less for subjects consuming the MCT product versus the subjects given carbohydrate alone under identical exercise protocols.
The results were as follows:
Glycogen Utilization Rate (mmol/kg/min)
MCT product 0.66
5.44
Carbohydrate
BLOOD PARAMETERS
Again, there was no significant difference between blood values of fatty acids, ketones, glucose or insulin at the end of the exercise bouts for all treatment groups. This indicates that fatty acids and ketones provided by the MCT product were used immediately as a source of energy.
Glycerol, the backbone of the triglycerides of capric acid, was present at high levels for subjects consuming the MCT product indicating that MCFAs were being removed from the triglyceride backbone during the exercise protocol.
EXAMPLE 4
After consumption of 100 grams of the MCT product of Example 1 or the caloric equivalent of carbohydrate only, glycogen use rates were compared during similar exercise protocols at 65% of V02max for 60 minutes.
The test protocol was similar to Example 1, however four subjects were tested at 65% of V02max for 60 minutes. MUSCLE GLYCOGEN USE The amount of muscle carbohydrate used per minute during exercise was slightly higher for subjects consuming the MCT product versus the subjects given carbohydrate alone under identical exercise protocols.
The results were as follows;
Glycogen Utilization Rate (mmol/kg/min)
MCT product 0.70
Carbohydrate 0.28
BLOOD PARAMETERS
Blood values of glucose and insulin at the end of the exercise bouts were slightly higher for all subjects consuming carbohydrate versus the MCT product as expected. Blood values of triglycerides, free fatty acids, glycerol, and ketones were not significantly different for either treatment group. This indicates that fatty acids were also provided by endogenous sources of triglycerides during this low intensity exercise.
EXAMPLE 5
After consumption of 100 grams of the MCT product of Example 1 or the caloric equivalent of carbohydrate only, glycogen use rates were compared during similar exercise protocols at 50% of V02max for 60 minutes.
The test protocol was similar to Example 1, however four subjects were tested at 50% of V02max for 60 minutes. MUSCLE GLYCOGEN USE
The amount of muscle carbohydrate used per minute during exercise was not significantly different for subjects consuming the MCT product versus the subjects given carbohydrate alone under identical exercise protocols. The results were as follows:
Glycogen Utilization Rate (mmol/kg/min)
MCT product 0.45
Carbohydrate 0.40
BLOOD PARAMETERS
Blood values of glucose and insulin at the end of the exercise bouts were slightly higher for all subjects consuming carbohydrate versus the MCT product as expected. Blood values of triglycerides, free fatty acids, glycerol, and ketones were not significantly different for either treatment group. This indicates that fatty acids were also provided by endogenous sources of triglycerides during this low intensity exercise.
EXAMPLE 6
Immediately after the exercise protocol described in Example 5, performance testing was measured as follows. After the post biopsy, subjects performed one-minute intervals at 115% of V02max with a 1:1 work:rest ratio. Subjects continued until they could not maintain a cadence of greater than 60 RPM or until they completed 10 intervals, whichever came first. The time of the performance test is the total number of seconds of work, not counting rest intervals. PERFORMANCE TESTING RESULTS
TEST TIME (seconds)
MCT product 308
Carbohydrate 145
All subjects that participated in the performance testing after riding for 60 minutes at 50% of V02max performed longer due to the ingestion of the MCT product prior to exercise.
Although the study was double-blind in nature, it was noted in the results that all subjects commented after completion of the study that the ride performed after only carbohydrate ingestion was significantly more difficult than the ride after the ingestion of the MCT
product. It was also noted that riders worked visibly harder during the carbohydrate performance test.
Although the rate of glycogen utilization was not different during the 60 minute ride at 50% of V02max, after exercise intensity was increased to 115% of V02max, there was a positive impact on performance time. The maintaining of muscle glycogen levels increased the exercise time before muscle exhaustion was manifested. This will decrease the time required for glycogen to be restored to capacity prior to the next physical activity, and increase the capacity to train more in a given period of time.
EXAMPLE 7
Immediately after the exercise protocol described in Example 4, performance testing was measured on only two of the four subjects as follows. After the post biopsy, subjects performed one-minute intervals at 115% of V02max with a 1:1 work:rest ratio. Subjects continued until they could not maintain a cadence of greater than 60 RPM or until they completed 10 intervals, whichever came first. The time of the performance test is the total number of seconds of work, not counting rest intervals. PERFORMANCE TESTING RESULTS
TEST TIME (seconds)
MCT product
Subject 1 227 Subject 2 600
Carbohydrate
Subject 1 176 Subject 2 600
All subjects that participated in the performance testing after riding for 60 minutes at 65% of
V02max performed longer due to the ingestion of the MCT product prior to exercise.
Again it was noted that both subjects commented after the completion of the study that the ride performed after only carbohydrate ingestion was significantly more difficult than the ride after the ingestion of the MCT product. One subject rode for 10 intervals, the maximum, for both products. It was also noted that riders worked visibly harder during the carbohydrate performance test. Although the rate of glycogen utilization was not different during the 60 minute ride at 65% of V02max, after exercise intensity was increased to 115% of V02max, there was a positive impact on performance time. The maintaining of muscle glycogen levels increased the exercise time before muscle exhaustion was manifested. This will decrease the time required for glycogen to be restored to capacity prior to the next physical activity, and increase the capacity to train more in a given period of time.
Claims (20)
1. A method of sparing muscle glycogen in a subject, which comprises increasing medium chain fatty acid in the subject for undergoing physical activity at an intensity greater than 70% of V02max.
2. A method as claimed in claim 1, wherein the medium chain fatty acid is increased by administering a triglyceride of at least one medium chain fatty acid to the subject.
3. A method as claimed in claim 1, wherein the medium chain fatty acid is increased by administering a triglyceride of three medium chain fatty acids to the subject.
4. A method as claimed in claim 3, wherein the medium chain fatty acids are saturated or unsaturated fatty acids having 6 to 12 carbon atoms.
5. A method as claimed in claim 4, wherein the medium chain fatty acids are saturated fatty acids having 6 to 12 carbon atoms.
6. A method as claimed in claim 5, wherein the medium chain fatty acids are saturated fatty acids having 10 carbon atoms.
7. A method as claimed in claim 2, wherein the triglyceride is administered by ingestion.
8. A method as claimed in claim 2, wherein the triglyceride is administered together with a carbohydrate.
9. A method as claimed in claim 1, wherein medium chain fatty acid is increased before the subject undergoes the physical activity.
10. A method as claimed in claim 1, wherein medium chain fatty acid is increased while the subject undergoes the physical activity.
11. A method as claimed in claim 2, wherein the triglyceride is administered in an amount effective for sparing muscle glycogen.
12. A method as claimed in claim 3 , wherein the triglyceride is administered in an amount effective for sparing muscle glycogen.
13. A method as claimed in claim 12, wherein the amount is 1 to 500 grams.
14. A method as claimed in claim 13, wherein the amount is 10 to 25 grams.
15. A method as claimed in claim 1, wherein the subject is a human.
16. A method as claimed in claim 1, wherein the physical exercise intensity is at least 75% of V02max.
17. A method as claimed in claim 1, wherein the physical exercise intensity is at least 85% of V02max.
18. A method as claimed in claim 3, wherein the triglyceride is administered in solid form.
19. A method as claimed in claim 3, wherein the triglyceride is administered in liquid form.
20. A method of enhancing physical performance capacity of a subject, which comprises administering to the subject a triglyceride of medium chain fatty acids for undergoing physical activity at an intensity greater than 70% of V02 max.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US5603593A | 1993-04-30 | 1993-04-30 | |
US056035 | 1993-04-30 | ||
PCT/US1994/004796 WO1994025019A1 (en) | 1993-04-30 | 1994-04-29 | Enhancing performance capacity by sparing muscle glycogen with medium chain fatty acids |
Publications (1)
Publication Number | Publication Date |
---|---|
AU6779294A true AU6779294A (en) | 1994-11-21 |
Family
ID=22001735
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AU67792/94A Abandoned AU6779294A (en) | 1993-04-30 | 1994-04-29 | Enhancing performance capacity by sparing muscle glycogen with medium chain fatty acids |
Country Status (4)
Country | Link |
---|---|
EP (1) | EP0706390A4 (en) |
AU (1) | AU6779294A (en) |
CA (1) | CA2161784A1 (en) |
WO (1) | WO1994025019A1 (en) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2005120484A1 (en) * | 2004-06-09 | 2005-12-22 | Kurume University | Regulator for physiological functions of ghrelin |
CN106793800A (en) * | 2014-10-10 | 2017-05-31 | 雀巢产品技术援助有限公司 | For strengthening motility or activity or treating weak composition and method |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5747446A (en) * | 1980-09-05 | 1982-03-18 | Nippon Oil & Fats Co Ltd | Nutrious food composition |
US4687782A (en) * | 1984-12-10 | 1987-08-18 | Nutri-Fuels Systems, Inc. | Nutritional composition for enhancing skeletal muscle adaptation to exercise training |
FR2668039B1 (en) * | 1990-10-18 | 1993-06-25 | Pernod Ricard | DIETETIC DRINK INTENDED TO SUPPORT EFFORT. |
GB9121467D0 (en) * | 1991-10-10 | 1991-11-27 | Sandoz Nutrition Ltd | Improvements in or relating to organic compounds |
EP0537113A1 (en) * | 1991-10-10 | 1993-04-14 | SANDOZ NUTRITION Ltd. | Energy supplementary food |
-
1994
- 1994-04-29 EP EP94915965A patent/EP0706390A4/en not_active Withdrawn
- 1994-04-29 CA CA002161784A patent/CA2161784A1/en not_active Abandoned
- 1994-04-29 WO PCT/US1994/004796 patent/WO1994025019A1/en not_active Application Discontinuation
- 1994-04-29 AU AU67792/94A patent/AU6779294A/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
EP0706390A4 (en) | 2000-08-23 |
EP0706390A1 (en) | 1996-04-17 |
CA2161784A1 (en) | 1994-11-10 |
WO1994025019A1 (en) | 1994-11-10 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Spriet et al. | Nutritional strategies to influence adaptations to training | |
Wright et al. | Carbohydrate feedings before, during, or in combination improve cycling endurance performance | |
Kreider | Dietary supplements and the promotion of muscle growth with resistance exercise | |
Sherman | Metabolism of sugars and physical performance | |
Millard-Stafford et al. | Recovery from run training: efficacy of a carbohydrate-protein beverage? | |
Williams et al. | Effects of recovery beverages on glycogen restoration and endurance exercise performance | |
Van Essen et al. | Failure of protein to improve time trial performance when added to a sports drink | |
Greer et al. | Branched-chain amino acid supplementation and indicators of muscle damage after endurance exercise | |
CA2334415C (en) | Compositions for increasing energy in vivo | |
Raastad et al. | Omega‐3 fatty acid supplementation does not improve maximal aerobic power, anaerobic threshold and running performance in well‐trained soccer players | |
Niles et al. | Carbohydrate-protein drink improves time to exhaustion after recovery from endurance exercise. | |
RU2503269C1 (en) | Muscular proteins synthesis enhancement method | |
US6429198B1 (en) | Compositions for increasing athletic performance in mammals | |
RU2492705C2 (en) | FOOD ADDITIVE THAT CONTAIN α-KETO ACIDS | |
Lawrence et al. | Feeding status affects glucose metabolism in exercising horses | |
Murdoch et al. | Differences in the effects of carbohydrate food form on endurance performance to exhaustion | |
Manetta et al. | Substrate oxidation during exercise at moderate and hard intensity in middle-aged and young athletes vs sedentary men | |
Antonio et al. | Supplements for endurance athletes | |
Aras et al. | The effects of active recovery and carbohydrate intake on HRV during 48 hours in athletes after a vigorous-intensity physical activity | |
AU6779294A (en) | Enhancing performance capacity by sparing muscle glycogen with medium chain fatty acids | |
Cinar et al. | The effect of magnesium supplementation on glucose and insulin levels of tae-kwan-do sportsmen and sedentary subjects. | |
Hasson et al. | Effect of carbohydrate ingestion on exercise of varying intensity and duration: practical implications | |
Silva | The Effects of One-Week Exogenous Ketone Consumption on Time Trial Running Performance | |
Peepathum et al. | Effects of Osmolality of Rice Sports Drinks on Sports Performance | |
Malmivaara | The effect of high-intensity interval exercise program on blood lipids and hormones in recreationally active adults |