AU669027B2 - Salt taste enhancers - Google Patents

Description

I II I -ce 6690 7 -1-

AUSTRALIA

PATENTS ACT 1990 COMPLETE SPECIFICATION S P E C I F I C A T 1 0 N FOR A STANDARD PATENT rrre

I

cae r rr

I:

rict ririr r rr r r Ir r r r

ORIGINAL

c 4444 '4,4 4(44 V C Ct t S 4( 4- 4 Name of Applicant: Actual Inventors: Address for Service: SOCIETE DES PRODUITS NESTLE S.A.

Eldon Chen-Hsiung LEE and John Stewart TANDY SHELSTON WATERS 55 Clarence Street SYDNEY NSW 2000 Invention Title: "SALT TASTE ENHANCERS" The following statement is a full description of this invention, including the best method of performing it known to us:la SALT TASTE ENHANCERS The present invention relates to salt taste enhancers more particularly to encapsulated ammonium salts as salt enhancers for food compositions.

Excessive dietary sodium ion intake, the primary source of which is sodium chloride or table salt in foods, has long been associated with a number of health problems such as hypertension. It is generally recognized that the sodium ion intake of most persons is in excess of 0 minimal physiological needs of the body. Consequently a marked reduction in sodium consumption is recommended for most persons. However, the inclusion of sodium chloride in the diet contributes a good deal to the palatability of foods, and food without salt is 15 perceived to be tasteless, flat and unpalatable.

Heretofore, a number of sodium-free compositions have been suggested as salt substitutes to replace sodium chloride in foods while retaining the palatability of the food.

Examples of such salt substitutes are disclosed in US patent Nos. 2,471,144; 2,601,112; 3,782,974; 4,243,691; 4,340,614; and 4,451,494. Among the most popular salt substitutes are potassium chloride, ammonium salts such as ammonium chloride and mixtures thereof. However, such salt substitutes suffer from a number of disadvantages, including off-taste or bitter flavor, a taste perception different than sodium chloride and a salty impression much less than that of sodium chloride. In particular, ammonium salts are hygroscopic and have a sour aftertaste. Typically a number of other components must be included to mask the bitterness which the salt P.!l -2substitute, such as potassium or ammonium chloride imparts, such as the combination with potassium chloride of calcium and magnesium formate and citrate salts, sugar, choline citrate and hydrolysed animal protein.

Another procedure which has been suggested for reducing sodium ion intake is to incorporate salt taste enhancers in foods and beverages. That is, compounds, which 'potentiate or amplify the taste of sodium chloride in foods and beverages so that the sodium chloride content thereof may be reduced without adversely affecting the desired salty taste of the food. For example, US patent No. 4,997,672 and the prior art discussed therein, disclose the use of substances such as cationic surfactants, bretylium tosylate, certain polypeptides, and the like as salt taste enhancers.

We have found that an encapsulated ammonium salt, when added to a food or beverage containing less than a normal amount of sodium chloride, will enhance or potentiate the salty taste of the food or beverage.

Accordingly, the present invention provides a composition enhanced in sodium chloride taste which comprises a food or beverage containing a less than normal amount of sodium chloride, and a sodium chloride taste potentiating amount of an encapsulated ammonium salt, the composition having a sodium chloride content of at least 0.20% by weight.

Examples of ammonium salts which may be encapsulated are food acceptable salts such as the chlorides, phosphates, citrates, lactates, tartrates, fumarates, adipates, malates, 0 S succinates and gluconates.

*4 o a 4, -7 4q~ft 9*4* f *tt* ft C it Ct 3 One useful source of ammonia which can be converted into an ammonium salt to be encapsulated is ammonia recovered from fermented soy sauce during spray drying.

However, one especially advantageoLs source of ammonia which can be converted into an ammonium salt is formed during protein hydrolysis, e.g. from the evaporator condensate waste stream obtained in the production of acid hydrolysed proteins. In this acid hydrolysis process, vegetable plant or animal proteins derived from 10 corn, soy, wheat, rice, yeast, peanut or casein are commonly used as starting protein sources and are usually obtained as a result of the separation of the protein fraction during milling of grains or following solvent extraction of'oils. The protein contents of these raw materials may range from 40% to 90% with a general average of about 60%. Normally, the protein source is hydrolysed with hydrochloric acid having a concentration of about 20% by weight at a temperature from about 1200 135 0 C over a period from about 5 to 8 20 hours and elevated pressure up to 30 psig (2 bar).

Following hydrolysis, the slurry is neutralised with a suitable alkaline material such as sodium hydroxide or sodium carbonate to a pH from 5.0 to 5.3 and the residual unhydrolysed material (lignin, humin) filtered out. The slurry may be decolourised prior to filtration or the filtrate following filtration may be decolourised by conventional means e.g. activated carbon, absorption resins. Following filtration of the unhydrolysed material and salts formed, the filtered liquid containing about 42% solids may be further concentrated to pastes by a vacuum evaporator and the pastes then dried in a vacuum oven. Ammonia is removed during vacuum evaporation and may be trapped in the distillate by a condensor forming an evaporator condensate waste stream containing dilute ammonia.

i -4 The encapsulated ammonium salt may be produced from the evaporator condensate waste stream by an evaporator system or by a degassifier system to give a concentrated ammonium salt which may then be spray dried with the encapsulating agent.

The present invention further provides an encapsulated ammonium salt wherein the ammonium salt is derived from ammonia formed during protein hydrolysis.

In the evaporator system, the encapsulated ammonium salt enhancer is prepared by neutralising the evaporator condensate waste stream with an acid hydrochloric acid), concentrating using a vacuum evaporator, purifying with activated carbon, filtering and then spray drying the filtrate with an encapsulating agent.

In the degassifier system, the encapsulated ammonium salt enhancer is prepared by heating the evaporator condensate waste stream to a temperature of from 30 0 C to just below the boiling point of water, preferably from 0 C to 96 0 C and more preferably from 40 to 90 0

C,

20 through a heat exchanger, raising the pH to above 9, i o ft preferably above 10 and especially 11 with alkali, injecting through a nozzle into a packed tower followed S" by a forced air degassifier, trapping the volatiles in 9 an acid solution, e.g. concentrated HC1 phosphoric, tartaric, lactic or citric acid to form a salt solution and then spray drying the salt solution containing usually from 15% to 25% by weight of solids with an encapsulating carrier.

Encapsulating agents that may be used include 30 maltodextrin, gum arabic, and gelatinised starches which are advantageously hydrolysed.

5 The starches are preferably starches with a high amylopectin content such as waxy cereal starches e.g.

waxy maize starch and waxy rice starch. The starches are conveniently cooked to be gelatinized before hydrolysis and, if desired, they may be modified, preferably at the raw stage. The starches may be hydrolysed at their 1,6-alpha-D-glucosidic linkages.

The hydrolysing enzyme may, for instance, be an isoamylase (1,6-alpha-D-glucosidase), a debranching s o 10 enzyme which specifically hydrolyses the o*i 1,,6-alpha-D-glucoside bonds of branch-chain amylopectin to form amylose without formation of reducing sugars and/or oligosaccharides. Isoamylases may be obtained from broad beans (R-enzyme) or from the fermentation of yeast and bacterial species such as Pseudomonas and Cytophaga. The isoamylase, Amano DB-250 (Amano Enzyme USA Co.Ltd) is obtained by a fermentation process from a selected strain of Bacillus sectorramus. Another suitable hydrolysing enzyme is a heat-stable pullulanase 20 debranching enzyme, pullulan 6-glucano-hydrolase, which hydrolyses the 1,6-alpha-D-glucosidic linkages of amylopectin as well as pullulan, a linear polysaccharide composed of maltotriose units linked by 1,6-alpha-D-glucosidic bonds. The pullulanase, Amano pullulanase ;t 3 (Amano Enzyme USA Co, Ltd) is obtained by a fermentation process from a selected strain of Aerobacter sp. The pullulanase, Novo promozyme 200L (Novo Nordisk A/S) is obtained from a selected strain of Bacillus acidopullulyticus by submerged fermentation and is a U.S. food grade enzyme, which has been used for preparing the debranching starch hydrolysate as the encapsulating agent.

The encapsulated ammonium salt taste enhancers of the present invention are effective in potentiating or 6amplifying the salty taste of foods and beverages even when incorporated at relatively low levels. For example, the salty taste of foods and beverages is significantly enhanced by the incorporation therein of encapsulated ammonium salt containing the ammonium salt in an amount of from 0.05 to 0.75% preferably from 0.1 to 0.5% and especially from 0.15 to 0.3% by weight based on the weight of food or beverage. If the encapsulated ammonium salt contains about 50% by weight of the ammonium salt, 10 then the levels of the encapsulated ammonium salt are from 0.1 to preferably from 0.2 to 1.0% and especially from 0.3 to 0.6% by weight based on the weight of food or beverage. Thus, the salt taste enhancers of the present invention allow the sodium chloride content of a food or beverage to be reduced without adversely affecting the desired salty taste of the product. It is to be understood that the salt taste enhancers of this invention are not salt substitutes and do not completely replace sodium chloride in the food or 20 beverage. Rather, they are saltiness enhancers and require a minimum level of sodium chloride in the food or beverage of about 0.20% in order to potentiate the sodium chloride taste in the product.

A salt taste potentiating amount of encapsulated ammonium salt is effective in potentiating the sodium chloride taste in a wide variety of foods and beverages containing a less than normal amount of sodium chloride, but having a minimum level of sodium chloride of at least about 0420%. For example, the salty taste of foods such as low sodium chicken broth, soups, salad dressing, sauces, mayonnaise, cooked ground beef, oat-meal, and the like, containing a less than normal amount of sodium chloride, is significantly enhanced by the addition of encapsulated ammonium salt in the amounts indicated i} 35 above. Greater amounts up to about 3% or more of the 7encapsulated salt may of course, be used but, preferably, the amount of encapsulated salt used is from 0.1% to 1.5% by weight based on the weight of food or beverage as indicated above.

Accordingly, the present invention provides, in addition, a process for potentiating the salty taste of a food or beverage containing a less than normal amount of sodium chloride which comprises adding to the said ooo* food or beverage a sodium chloride taste potentiating amount of an encapsulated ammonium salt.

The present invention also provides a process for i. preparing a salty tasting food or beverage containing a reduced amount of sodium chloride which comprises formulating a food or beverage with a reduced amount of 15 sodium chloride less than is necessary to achieve a desired salty taste in said food or beverage, and potentiating the sodium chloride taste in said reduced sodium chloride content food or beverage by adding thereto an encapsulated ammonium salt.

20 The following Examples further illustrate the present invention. Parts and percentages are by weight when not stated.

Examiple 1 Waxy maize starch was dispersed in an aqueous solution, cooked to 95 0 C, and then cooled to 40°C in a Brabender amylograph. Amano pullulanase 43 (Amano Enzyme USA Co. Ltd) (3,000 units/ml) was added at the use level of 0.2% based on starch. The hydrolysis reaction was carried out with agitation at a constant temperature, 40 0 C and viscosity is monitored during reaction.

8 HPP evaporator condensate (100 kg, ammonium nitrogen 0.210%) was neutralized with 32% hydrochloric acid, concentrated using a vacuum evaporator to 3kg, purified with activated carbon (100 g Nuchar SA) at 70 0 C for min., and then filtered. The filtrate (ammonium nitrogen 5.79%; NH 4 C1 22.12%) was mixed with the above starch hydrolysate (2,27 kg), and then spray-dried with inlet air temperature of 150 0 C and outlet temperature of to finished powder product (NH 4 Cl The recovery i 10 yield of ammonium chloride during spray drying was 68.7%. The product gave an excellent clean, white powder Swith low hygroscopicity.

Example 2 Waxy rice starch was used instead of waxy maize starch in the Example 1 and the isoamylase alpha-1,6-D-glucosidase (Amano DB-250-Amano Enzyme USA Co. Ltd) (300 units/ml) was used instead of pullulanase at the use level of 2% based on starch. The resulting finished powder product contained 50.0% NH4CL. The recovery yield of ammonium chloride was 74.0%. The finished product gave an excellent clean, white and low Shygroscopic powder.

Example 3 A typical low-cost cheese sauce contains approximately 1.6% by wt. sodium chloride. In order to demonstrate the effectiveness of the salt taste enhancers of the present invention, a comparable low-cost cheese sauce was formulated containing 0.5% sodium chloride, and was used as a control. To one aliquot of this control cheese sauce was added 0.40% by weight of the spray dried encapsulated ammonium chloride (48% NH 4 C1 by weight) Sprepared in Example 1. The control cheese sauce and the sauce to which the salt enhancers had been added were then evaluated by a sensory panel consisting of eight trained judges who were of the opinion that the salt enhancer significantly amplified the sodium chloride taste.

Example 4 3.41g of a low sodium chicken base containing 41 mg Na/g base and a 1.69 g of a low sodium beef base containing 83 mg Na/g base were used as test media each in a 10 serving portion of 100g.

Control samples were made by adding NaC1 and KC1 to the test media and a sample according to the present invention was made by adding 0.40g of the spray dried encapsulated ammonium chloride (48% NH 4 C1 by weight) as 15 prepared in Example 1. The amounts of NaC1 and KC1 as well the sodium content 100 g serving portion are .t given in the following Table 1.

it c 10 Table 1 Sample Sodium content 100 g serving chicken broth Beef broth 4**A 40*00*

A

1) Test medium 5 2) Control with NaCl added 3) Encapsulated ammonium salt 4) KCl 140 mg 280 mg 140 mg 14 0mg 3.41g base 3.41g base +0.36g NaCi 3.41g base 40g 3.41g base 46g 1.69g base 1.69g base +0.36g NaCl 1.69g base +0.40g 1. 69g base +0.46g PA A C C A AC

*.ACC*

A sensory panel judged that the encapsulated ammonium salt significantly amplified the sodium chloride taste; the sample gave comparable salt taste to the control sample and gave better salt taste than the potassium is chloride sample which had an undesirable aftertaste.

Claims (11)

1. A composition enhanced in sodium chloride taste which comprises a food or beverage containing a less than normal amount of sodium chloride, and a sodium chloride taste potentiating amount of an encapsulated ammonium salt, the composition having a sodium chloride content of at least 0.20% by weight.

2. A composition according to claim 1 wherein the ammonium salt is the chloride, phosphate, citrate, lactate, tartrate, fumarate, adipate, malate, succinate or gluconate.

3. A composition according to claim 1 wherein the ammonium salt is obtained from ammonia recovered by spray drying fermented soy sauce.

4. A composition according to claim 1 wherein the ammonium salt is obtained from ammonia formed during protein hydrolysis. A composition according to claim 1 wherein the ammonium salt is obtained from ammonia derived from the evaporator concentrate waste stream formed in the production of acid hydrolysed proteins.

S***m 15

6. A composition according to any one of claims 1 to 5 wherein the ammonium salt is present in an amount of from 0.05 to 0.75% by weight based on the weight of the food or beverage. t. t t t

7. A composition according to any one of claims 1 to 6 wherein the encapsulated S ammonium salt is encapsulated by means of a gelatinised starch hydrolysed at its 1,6- alpha-D-glucosidic linkage by isoamylase, pullulanase or pullulan 6-glucano-hydrolase.

8. A process for potentiating the salty taste of a food or beverage containing a less Sthan normal amount of sodium chloride which comprises adding to the food or beverage a sodium chloride taste potentiating amount of an encapsulated ammonium salt, the food or beverage having a sodium chloride content of at least 0.20% by weight.

9. A process for preparing a salty tasting food or beverage containing a reduced Samount of sodium chloride which comprises formulating a food or beverage with a -12- reduced amount of sodium chloride less than is necessary to achieve a desired salty taste in said food or beverage, and potentiating the sodium chloride taste in said reduced sodium chloride content food or beverage by adding thereto an encapsulated ammonium salt, the food or beverage having a sodium chloride content of at least 0.20% by weight.

10. A composition enhanced in sodium chloride taste, substantially as herein described with reference to any of the Examples.

11. A process for preparing a composition enhanced in sodium chloride taste, substantially as herein described with reference to any one of the Examples. DATED this 4th day of March, 1996 SOCIETE DES PRODUITS NESTLE S.A. Attorney: RUTH M. CLARKSON Fellow Institute of Patent Attorneys of Australia of SHELSTON WATERS t L t t iit C t t I ABSTRACT A composition enhanced in sodium chloride taste which comprises a food or beverage containing a less than normal amount of sodium chloride, and a sodium chloride taste potentiating amount of an encapsulated ammonium salt especially wherein the ammonium salt is obtained from ammonia formed during protein hydrolysis. sees 000* o o o 0 a 0* o 5550 *a *e e«6 a« *c 0 .9.5 C0 0 k~j L