AU668033B2 - Colostrum preparation and storage - Google Patents
Colostrum preparation and storage Download PDFInfo
- Publication number
- AU668033B2 AU668033B2 AU63136/94A AU6313694A AU668033B2 AU 668033 B2 AU668033 B2 AU 668033B2 AU 63136/94 A AU63136/94 A AU 63136/94A AU 6313694 A AU6313694 A AU 6313694A AU 668033 B2 AU668033 B2 AU 668033B2
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- AU
- Australia
- Prior art keywords
- colostrum
- ultra
- subjecting
- filtration
- product
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/20—Dietetic milk products not covered by groups A23C9/12 - A23C9/18
- A23C9/206—Colostrum; Human milk
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- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Dairy Products (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Description
AUSTRALIA
Patents Act 668 03 3 COMPLETE SPECIFICATION
(ORIGINAL)
Class Int. Class Application Number: Lodged: Complete Specification Lodged: Accepted: Published: Priority Related Art:
F-
Av1A -1 4444 o oC 64441 4 Name of Applicant: Adbe7" KI"1...
lr*< i-Y zc76,~a~s i 2ic~.
/11 I I ~v~f~rP-T-~vP1Ii.~.Ci 4" Actual Inventor(s): 0Z4- 0.
Peter Bennett Duff Whyte Address for Service: PHILLIPS ORMONDE FITZPATRICK Patent and Trade Mark Attorneys 367 Collins Street Melbourne 3000 AUSTRALIA Inwmtion Title: COLOSTRUM PREPARATION AND STORAGE Our Ref 368662 POF Code: 114677/115908 The following statement is a full description of this invention, including the best method of performing it known to applicant(s): -1- 2 This invention relates to a process for the preparation and storage of colostrum products. It is particularly applicable to preparing and storing antibody enriched colostrum without loss of the antibody activity.
Colostrum is the milk secreted by a mammal just before and for a short period after giving birth, containing antibodies to protect offspring against disease.
It has been recognised in the prior art that mammals such as cows may be immunized with specific antigens. The antibody enriched colostrum may then be harvested.
A difficulty in the prior art has been that there has been no economical process for preparing and storing colostrum products.
It is known in the prior art that milk products may be spray dried to give a powdered product which may be o stored. When known spray drying processes were used to process colostrum it was unexpectedly discovered that the high salt content of the colostrum was a problem which 0rendered known processes uneconomical.
20 Colostrum has an extremely high salt content. If known spray drying processes are used to process colostrum the spray drier must undergo extensive cleaning every day for approximately 10 hours to prevent corrosion of the drier. As indicated above this renders the process uneconomic and is a problem which does not relate to normal milk.
0 Accordingly, it is an object of the present invention to overcome or at least alleviate, one or more of the difficulties related to the prior art.
Accordingly as a first aspect of the present invention there is provided a process for the preparation of a colostrum product which process includes: providing colostrum; a process for the preparation of a colostrum product which process includes providing colostrum; removing or substantially reducing the salt content of said colostrum; subjecting the colostrum to a bactofugation step; 3and subjecting the colostrum to a spray drying process.
A further aspect of the present invention provides a process for the preparation of a colostrum product which process includes: providing colostrum; subjecting the colostrum to a bactc;jugtion step; subjecting the colostrum to an ul-ra-filtration process; and subjecting retentate of the ultra-filtered colostrum to a spray drying process.
The product so formed is provided in a preserved form without loss of antibody activity. Normal pasteurisation cannot be employed due to the extreme heat sensitivity of immuno-proteins. The ,.bactofugation step reduces the bacterial contamination o'f 'the product.
The inventors have found that the step of removal of salt from the colostrum renders the process economic.
E Salt removal is significant as it reduces the likelihood 20 of corrosion damage to stainless steel plant and reduces the necessity to wet wash drying equipment, offering a significant saving of time and money.
The colostrum may be freshly harvested colostrum or frozen colostrum. The colostrum may be bovine colostrum harvested from freshly calved cows. The colostrum may be harvested using modern milking equipment. The colostrum may be antibody enriched.
The ultra-filtration apparatus preferably removes all molecules of molecular weight less than 20,000 for example water, minerals, salts and non-protein nitrogen.
Any suitable ultra-filtration apparatus may be used. In a preferred embodiment a hollow fibre ultra-filtration plant is used. The ultra-filtration step may also remove or reduce the lactose from the content of the colostrum.
This is also advantageous from a medical point of view.
In a preferred aspect of the present invention the process may further include the preliminary step of pasteurisation. Low temperature, long time pasteurisation is preferably employed due to the extreme heat sensitivity
L
4 of immuno-proteins. In a preferred embodiment the pasteurisation is conducted at approximately 63 0 C for approximately 30 minutes. The pasteurised colostrum is then preferably cooled to approximately 55 0 C before undergoing the ultra-filtration step. The pasteurised colostrum may suitably be cooled by regeneration.
In a further preferred aspect of the present invention the process may further include the preliminary step of separating the colostrum. The colostrum may be preferably separated into a light phase (cream) and a heavy phase (skim). The light phase is preferably discarded.
Accordingly yet a further aspect of the present invention provides a process for the preparation of a colostrum product which process include,s providing colostrum; j 'subjecting the colostrum to a separation step to produce a light phase and a heavy phase; o subjecting the heavy phase colostrum to a S 20 bactofugation process; subjecting the bactofugated colostrum to an ultra-filtration process to remove or substantially reduce the salt content thereof; and subjecting the filtered colostrum to a spray drying process.
Any suitable separation apparatus may be used. A preferable separation apparatus is a self desludging type apparatus.
The colostrum is preferrably pre-heated to S 30 approximately 50 0 C prior to the separation step. Any suitable means may be used to preheat the colostrum, for example by means of a plate heat exchanger.
In a preferred embodiment it is introduced at a temperature of approximately 55 0 C to 65 0 C. A two-fluid nozzle may be used to introduce the concentrate into the drying chamber. In a preferred embodiment, the drying chamber is maintained at approximately 180°C inlet temperature and 70 0 C outlet temperature. The Applicants have unexpectedly found that this drying technique which would normally be expected to inactivate the antibodies, has been found by the Applicants not to do so. The energy of the spray drying is taken up as latent heat of evaporation, and accordingly there is very little particle temperature elevation. Accordingly heat damage is minimised. The dried particles preferably fall into a static fluidised bed where further drying and fines agglomeration take place. This produces a product with good dispersibility in water.
It will be understood that the steps of the process of the present invention as described above may take place in any suitable order. Other steps may also be included in the process of the present invention. For example in another preferred embodiment of the present application casein is removed from the milk by precipitation. This step preferably.-takes place after the colostrum has been subjected to a separation step and the o.o oo light phase has been discarded.
0o; The present invention will now be more fully described with reference to the accompanying drawings and example. It should be understood, however, that the description following is illustrative only, and should not 0ooo be taken in any way as a restriction on the generality of the invention described above.
In the drawings: 00* Figure 1 is a flow diagram of the process %o-ooo according to one embodiment of the present invent.ion.
EXAMPLE 1 o« o' Antibody enriched bovine colostrum was harvested 000000 30 from freshly calved cows using modern milking equipment.
The colostrum was quickly cooled and held under refrigeration (<4 0 C) until it was collected. The colostrum was collected daily in a refrigerated van Colostrum from individual farms was segregated to prevent cross-contamination.
The colostrum was weighed and tested for microbial quality, inhibitory substances and for specific antibodies to known pathogens. The colostrum was then frozen and stored at -20 0 C pending the results of these tests.
L 6 The frozen colostrum was thawed in a water bath then pooled according to antibody content, to produce a uniform product. The pool was kept refrigerated prior to processing.
The pooled colostrum was pre-heated to 55 0 C by means of a plate heat exchanger and separated in a self desludging type separator. The light phase (cream) was discarded and the heavy phase (skim) was bactofugated in a bacteria-removing centrifuge.
The skim was passed through a hollow fibre ultra-filtration plant to remove molecules of molecular weight less than 20,000; namely water, lactose, minerals, salts, non-protein nitrogen. The ultra-filtered retentate was preheated to reduce viscosity prior to spray drying.
The concentrate, at 58 0 C, was introduced into a spray drying chamber of aseptic design with hepa filters in the feed line, atomising air, and drying air supply, purpose built for pharmaceutical grade products. The S° drying chamber was maintained at 180 0 C inlet temperature o, i 20 and 70°C outlet temperature. The dried particles fell into a static fluidised bed where further drying and fines agglomeration took place, producing a product with good oo^ dispersibility in water. The finished product was then packaged.
EXAMPLE 2 Antibody levels and quantities of colostrum collected from cows varies dramatically over the first o" week, after calving. The colostrum from the first six t 30 milking was collected. For practical handling reasons all milk collected on one day was pooled at each farm with each farms milk kept separate by collection date. Each of these farm's milk deliveries was kept separate and assessed individually for quality. Colostrum quality was shown to be satisfactory with respect to microbial and inhibitory substances tests, and pooled on the basis of quantity (batch size) and antibody titre to produce a standard, weighted average antibody titre to a known pathogen. This pooling step ensured that a standard Li_ 7 product was consistently produced with respect to both the colostrum composition and antibody potency.
Approved colostrum was pooled for processing so as to provide correct antibody levels in the final powder product. The pooled colostrum was processed by skimming in a cream separator to reduce fat, bactofugation to reduce microbiological load, ultra filtration to concentrate protein and reduce lactose and mineral levels, including salt, and spray drying in an aseptic, pharmaceutical model spray drier with filtered air supply. Powder was packed in new food grade poly-ethylene bags with new fibre-board containers and sealed for transport.
Table 2 illustrates a production flow chart.
TABLE 2 Production Flow Chart: Raw colostrufn is received, Colostrum Receival weighed and stored pending quality control and release.
Approved milk is pooled according Pool raw to age and potency to produce a colostrum homogeneous batch of standard potency.
Cream (milk fat) is removed by Separation to separation in a standard dairy remove cream separator The skim colostrum is centrifuged Bactofugation to eliminate pathogens and reduce total microbial load L 1 ~1~5~ -tl I -8- Product is concentrated by reduction of water, lactose Ultrafiltration and electrolyte levels through ultrafiltration membranes. Salt content is reduced during this process.
Spray drying Product spray is dried in an aseptic spray drier.
Product is held in quarantine until approved for Bulk quality control microbiological quality, moisture level and potency.
Approved bulk product packaged into sachets. Packed Packing product is returned for quality control.
_Packed product is held in quarantine until approved for S.Finishd P k microbiological quality, moisture level, pack quality, Quality Control potency and documentation review.
00 0 EXAMPLE 3 Table 3 lists the constituents of a sample of colostrum product which has been prepared by the process of Example 1, Table 3 Colostrum Product Prepared by Process of Example 1 Moisture Fat 2.7 Solubility Index Af 0,1 SC C.\WINWORD\SIMONlNODELEtiM3136C).DOC 8a- Sc.Pts./Ex. Matter Protein (TN x 6.38)% Lactose Ash 500'0 Salt Whey Protein Nitrogen mg/g Dispersability Wettability (Secs) 2500 Aluminium mg/kg Penicillin Other Inhibitory Substances (As Pencillin I.U./mL) (1,U./mL) 71.0 14.3 0.48 56 60+, 60+, 3.4 <0.0025 <0.0025 44 #4 4, SC CAWINW0R0\SIM,0N M-0DMLET WI 36000.~C *41 1 ~"iriri=31"~"~~cPu~ I LPULIII~ 9 The above process reduces salt content by approximately 25%. First colostrum contains approximately 32 mmol/L of NaCl. If concentrated 7 fold using conventional means, the salt content of the powder would be 217 mmol/kg, which is equivalent to 1.25% of the total weight. This concentration of salt could seriously effect the stainless steel surfaces of the spray drier.
The process described reduces the salt content from 1.25% to less than 0.94% of the final powder, significantly reducing the risk of corrosion, and negating the need for frequent wet cleansing procedures as described.
For pooled colostrum (milkings 1-6, skimmed) the process reduces salt content by approximately 59%. Pooled colostrum contains approximately 22 mmol/L of NaCl. If concentrated 9 fold using conventional means, the salt ccntent of the powder would be 199 mmol/kg, which is equivalent to 1.15% NaCl of the total weight.
The process described reduces the salt content from 1.15% to less than 0.47% NaCI of the final powder, significantly reducing the risk of corrosion and negating frequent wet cleansing procedures.
Finally, it is to be understood that various other modifications and/or alterations may be made without departing from the spirit of the invention as outlined herein.
Claims (11)
1. A process for the preparation of a colostrum product which process includes: providing colostrum; subjecting the colostrum to a bacterial reduction step utilizing centrifugation; removing or substantially reducing the salt content of said colostrum by subjecting the colostrum to an ultra-filtration process; and subjecting retentate of the ultra-filtration colostrum to a spray drying process.
2. A process as claimed in claim 1 wherein a hollow fibre ultra-filtratation apparatus is used.
3. A process as claimed in claim 1 or claim 2 wherein said ultra-filtration process removes substantailly all molecules of molecular weight less than about 20,000.
4. A process as claimed in any preceding claim wheloi.in the ultra-filtration 20 process removes or reduces the lactose content of the colostrum. 4,4 4
5. A process as claimed in any preceding claim further including subjecting the colost'um to a pasteurisation process. S 25
6. A process according to any preceding claim wherein the bacterial reduciton step takes place prior to the ultra-filtration process. 4 i
7. A process as claimed in any preceding claim wherein the process includes a step of separating the colostrum into a light phase and a heavy phase.
8. A process as claimed in claim 7 wherein said light phase is discarded. SC CANNWORDISMONNOD LMB t11)6C9H,DOC I.4 il j -11-
9. A process as claimed in claim 7 or claim 8 wherein the separation step takes place prior to the bacterial reduction.
A process substantially as hereinbefore described with reference to any one of the examples or drawings.
11. A colostrum product produced by the process of any preceding claim. DATED: 5 January, 1996 PHILLIPS, ORMONDE FITZPATRICK Attorneys for: MINISTER OF PRIMARY INDUSTRIES I ii Z 0< SC C\\VNWORD\SIMONXWNODELEB61136C09,DOC Eh-- IIIU~I) LL ABSTRACT A process for the preparation of a colostrum product which process includes providing colostrum; removing or substantially reducing the salt content of said colostrum; subjecting the colostrum to a bacterial reduction step utilizing centrifugation; and subjecting the colostrum to a spray drying process. o os "3d
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AU63136/94A AU668033B2 (en) | 1994-05-16 | 1994-05-16 | Colostrum preparation and storage |
NZ26056894A NZ260568A (en) | 1994-05-16 | 1994-05-20 | Preparation of spray-dried colostrum which includes a bacterial reduction step utilising centrifugation |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AU63136/94A AU668033B2 (en) | 1994-05-16 | 1994-05-16 | Colostrum preparation and storage |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AU82527/91A Addition AU644468C (en) | 1990-08-17 | 1991-08-19 | Colostrum preparation |
Publications (2)
Publication Number | Publication Date |
---|---|
AU6313694A AU6313694A (en) | 1995-12-07 |
AU668033B2 true AU668033B2 (en) | 1996-04-18 |
Family
ID=3748003
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AU63136/94A Expired AU668033B2 (en) | 1994-05-16 | 1994-05-16 | Colostrum preparation and storage |
Country Status (2)
Country | Link |
---|---|
AU (1) | AU668033B2 (en) |
NZ (1) | NZ260568A (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0859552A1 (en) * | 1995-11-08 | 1998-08-26 | Northfield Laboratories Pty. Ltd. | Liquid colostrum for dairy products |
EP1889547A2 (en) | 1998-04-30 | 2008-02-20 | Numico Research Australia Pty. Ltd. | A food composition and method of using same |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU709952B2 (en) * | 1995-11-08 | 1999-09-09 | N.V. Nutricia | Liquid colostrum for dairy products |
AU741299B2 (en) * | 1998-04-30 | 2001-11-29 | N.V. Nutricia | A food composition and method of using same |
-
1994
- 1994-05-16 AU AU63136/94A patent/AU668033B2/en not_active Expired
- 1994-05-20 NZ NZ26056894A patent/NZ260568A/en not_active IP Right Cessation
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0859552A1 (en) * | 1995-11-08 | 1998-08-26 | Northfield Laboratories Pty. Ltd. | Liquid colostrum for dairy products |
EP0859552A4 (en) * | 1995-11-08 | 1999-04-28 | Northfield Lab Pty Ltd | Liquid colostrum for dairy products |
EP1889547A2 (en) | 1998-04-30 | 2008-02-20 | Numico Research Australia Pty. Ltd. | A food composition and method of using same |
Also Published As
Publication number | Publication date |
---|---|
AU6313694A (en) | 1995-12-07 |
NZ260568A (en) | 1996-05-28 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
HB | Alteration of name in register |
Owner name: NUMICO RESEARCH AUSTRALIA PTY LTD Free format text: FORMER NAME WAS: NORTHFIELD LABORATORIES PTY LTD |