AU663641B2 - A blood product, a method of producing the same and a method of determining the virus inactivation capacity of an inactivation treatment - Google Patents

Description

AUSTRALIA

Patents Act 1990 COM~PLETE SPECIFICATION STANDARD PATENT Applicant IMMUNO AKTIENGESELLSCHAFT Invention Title: A BLOOD PRODUCT, A METHOD OF PRODUCING THE SAJ'E AND A METHOD OF DETERMINING THE VIRUS INACTIVATION CAPACITY OF AN INACTIVATION

TREATMENT.

0 0 *0 00 .090 o .0.000 0 The following statement is a full description of this invention, including the best method of performing it known to me/us: I 2 The invention relates to a virus-inactivated blood product, a method of producing the same as well as a method of determining the virus-inactivating capacity of an inactivation treatment.

By blood products, products from human or animal blood or plasma are meant, which are intended for therapeutic, prophylactic or diagnostic applications.

Such products may contain enzymes, proenzymes including coagulation factors, enzyme co-factors, enzyme inhibitors, immunoglobulins, albumin, plasminogen, fibrinogen, fibronectin or plasma.

The administration of blood products involves the risk of infection due to infectious agents possibly present in the donor plasma, such as hepatitis or AIDS viruses. Even if exclusively plasma that has been S. tested for its absence of these infectious agents is used, the danger that a patient might be infected cannot be excluded because of the limited sensitivity of the test methods. When producing blood products one is therefore forced to inactivate possibly present infectious agents by various methods.

There exists a great number of publications concerned with the inactivation of pathogens in blood products.

:The various methods include: heating the blood products in aqueous solution, possibly with the addition of virucidal Il--pP

I

3 substances, heating the blood products in aqueous solution in the presence of stabilizing agents treating the blood products with organic solvents and/or detergents, heating the blood products in dry and wet states, combined treatment of the blood products with an organic solvent/detergent and heating the blood products in the dry state.

All these inactivation methods aim at eliminating the potential infectiousness of the preparations, while maintaining their biological activity as far as possible. However, so far this aim could only be achieved in the case of albumin preparations, by heating aqueous albumin solutions at a temperature of 60°C for 10 h, because albumin is substantially more stable with regard to the influence of heat than all the other blood proteins.

In detail, the following publications may, e.g., "be mentioned to the prior art: DE-A- 29 16 711 describes a method of treating coagulation-factors-containing preparations in aqueous solution by applying a temperature of from 30 to 100°C, wherein an amino acid or a mono-, oligosaccharide or sugar alcohol are admixed to the solution of the coagulation factors.

053 338 describes a method of inactivating I 1-

I

4 hepatitis viruses in preparations containing factors IX and X, wherein the aqueous solution of a blood preparation is heated in the presence of calcium ions and, possibly, an amino acid and/or a saccharide or a sugar alcohol at temperatures of up to 100*C.

In EP-A2-0 035 204 a method of inactivating aqueous protein solutions, which may contain factor VIII, fibronectin, globulin, fibrinogen and other proteins, is disclosed, wherein the composition is mixed with a polyol and the mixture is heated to a temperature of from 60 to 75 0

C.

In EP-A2-0 052 827 a method of inactivating hepatitis viruses in an aqueous solution containing factors II and VII in the presence of a c' Icting agent and, possibly, an amino acid and/or a saccharide or a sugar alcohol is described.

In US-A-4,379,085 a method for the thermal inactivation of a plasma protein, such as C 1 -inhibitor or factor IX, in aqueous solution in the presence of potassium or ammonium citrate is disclosed.

In EP-A2-0 077 870 an inactivation method is described, in which an aqueous, factor VIII-containing solution is heated with amino acids, monosaccharides, oligosaccharides, sugar alcohols and hydrocarbon- or hydroxy-hydrocarbon-carboxylic acids having from 3 to carbon atoms, to a temperature of from 50 to In the PCT application WO 83/04371 a method of II- I 5 inactivating hepatitis viruses is disclosed, wherein a preparation containing the virus is treated at a temperature of from 4 to 40 0 C with a halohydrocarbon, in particular chloroform.

EP-Bl-0 015 055 discloses a method of treating a blood product, wherein the product is subjected to a microwave radiation treatment in the anhydrous condition so as to inactivate any microorganisms present.

In a treatise of the XII. International Congress on Blood Transfusion, Abstracts, "MIR" Publishers, Moscow 1969, pp. 473-475, Rosenberg et al. disclose a method of inactivati l albumin-containing preparations and fibrinogen in the dry state by heating for 10 hours at EP-A2-0 094 611 discloses a method of treating a factor VIII-containing composition in the dry, for instance the lyophilised, state by applying a temperature of at least 60*C for inactivating any hepatitis viruses present.

e The published PCT application WO 82/03871 describes a method of treating preparations containing blood coagulation enzymes, the preparations being heated in the dry state so as to inactivate infectious viruses present; the dry state is defined to be a state with less than 5 by weight (0,05) of water.

It has shown that the method of dry-heating a s~ -a p, I-s~ T-C I_ 6 lyophilised factor VIII concentrate at 60 0 C for 10 h does cause a certain virus inactivation, yet that hepatitis and also AIDS viruses are transmitted by administering these dry-heated products (Eur. J.

Epidemiol. 3, 103-118 (1987)). To increase the effectiveness of dry-heating, PCT application WO 88/08710 proposes a sequence of heat treatments.

Likewise, in EP-A-0 378 208, protein-containing compositons are subjected to a treatment with trialkyl phosphate in combination with a dry heating treatment.

The method of EP-B-0 159 311 suggests a treatment of blood products in the solid, wet state. A content of water, methanol or ethanol of more than 0.05 (5 by weight) and less than 0.70 (70 by weight) is adjusted and heating is effected in a closed container at a eeee: temperature in the range of from 50 to 121°C.

EP-B-0 050 061 discloses a method comprising the treatment of biological and pharmaceutical products with from 0.25 to 10 by weight of a non denaturating amphiphile (detergent). In the following the detergent concentration is understood to be a percentage by weight. In EP-B-0 131 740 it is, however, shown that the treatment with a detergent alone is relatively ineffective with regard to the virus inactivation. That publication suggests a mixture of a detergent and a di- 0 or trialkylphosphate for an effective treatment. In that instance, the concentration of the detergent was ~ae e~~8s da~~lI II~ I;_ 7 1% and that of the solvent was 0.1%.

A combined treatment with an organic solvent/detergent and with heat, wherein the blood product is heated in the dry state, has also been documented in the literature (American Journal of Hematology 35, 142 (1990).

Today, virus inactivation methods are termed as effective, if after application of the method on a blood product srmple admixed with a high dose of a test virus corresponding to a maximum possible titer of approximately 105 in a coagulation factor preparation) it is no longer possible to detect any viruses, the virus titer thus having been reduced to below the detection limit.

As a measure of inactivation, the so-called S...o *0 reduction factor is known, which is calculated after a single addition of the test virus from the decadic logarithm of the quotient of the initial and final virus titers. From the directive EC III/8115/89-EN of the Commission of the European Communities, furthermore the so-called total reduction factor is known. It is calculated from the sum of the reduction factors of 0 individual subsequent inactivation measures.

In modern medicine it is necessary to administer many blood products over long periods of time in many cases even as a permanent treatment in large quantities, also for prophylactic reasons. This will I s 8 necessarily lead to a cumulation of infectious particles and thus to a substantially increased risk of infection, even if preparations that have already been virus-inactivated are administered.

Reduction factors must be compared with the socalled "worst case situation" for a virus contamination of the entire plasma pool. From Zeitschrift fur Allgemeine Medizin 65, 429-433 (198F) it is known that despite the use of plasma that has been tested and found to be HIV negative, an HIV content of 105 ID/ml (infectious units per milliliter) is possible in plasma derivatives. Assuming that a patient is administered 100 1 of a factor VIII preparation in the course of his life, a method of virus-inactivating plasma derivatives must thus permit a virus titer reduction of at least 10 10 so as to avoid the infection of a patient with AIDS viruses.

The invention has as its object to provide a virus-safe blood product, exclusive of albumin, from which it can be expected that the transmission of infectious agents is excluded even if large quantities of blood product are administered, and which nevertheless still has a high biological activity.

According to one embodiment of the invention, a.

this object is achieved by a blood product, exclusive 0 of albumin, inactivated relative to infectious agents, which blood product conforms to a total virus reduction factor of a re 9 at least 40, and has a biological activity of at least based on the activity prior to carrying out the inactivation of the infectious agents, to be obtained by an inactivation treatment in which a) the blood product is treated in an aqueous solution containing at least 2 preferably at least 5 of a detergent, and subsequently is heated in the solid state, or b) the blood product is heated in the solid state and subsequently is treated in an aqueous solution containing at least 2 preferably at leist 5 of a detergent.

Preferably, the invention consists in a blood product inactivated relative to infectious agents, wherein the blood product is treated in an aqueous o. I solution containing more than 10 of a detergent and is heated in the solid state.

aAnother embodiment consists in a blood product, exclusive of albumin, inactivated relative to infectious agents, which blood product cc'nforms to a oo total virus reduction factor of at least 40, and has a biologic activity of at least 50 based on the activity prior to carrying out the inactivation of the infectious agents, to be obtained by an inactivation treatment comprising two or more different inactivation methods, wherein at least one method consists in a heat treatment of the blood product in solid and dry state 10 having a water content of from 0.05 to 0.70. In this embodiment, advantageously at least one method is to be a treatment with an aqueous detergent solution.

The biologic activity is determined as che enzymatic activity of blood clotting enzymes and co-factors), as avidity (of immunoglobulins) or as antigenic activity possibly by the use of activity or antigen markers.

The blood product according to the invention may be produced from conventional blood products by carrying out the inactivation treatment for a period of time which suffices to obtain a total virus reduction factbr of at least 40. This period of time may be determined experimentally at a blood product sample, by repeatedly adding certain amounts of test virus during the treatment, each repetition being effected only when the virus titer has decreased to a certain value, preferably to below the detection limit. The total virus reduction factor results from the su- of the "individual reduction factors.

r Thus, if test virus is added repeatedly at chosen time intervals to a biological product during the eoe.* treatment for virus inactivation, after determination of the initial and final virus titer, the decadic logarithm of the virus titer ratio may be multiplied by

S

the number of intervals and the reduction factors may be added up to a total virus reduction factor. This ~s I raLPIL% IIII~ L~es-e~~ ~SI 11 calculation requires that the virus titer reduction after the final test virus addition does not exceed the previous titer reductions, which also has shown to be so.

As the test virus, the AIDS virus or the Sindbis virus (as the model virus for hepatitis viruses) may be used.

The invention is based on the finding that a treatment with Tween or a detergent according to the prior art which is carried out at detergent concentrations of below 10 does not yield a satisfactory result, if the blood product is not subjected to a further method of virus inactivation.

"This may be due to a protective effect of proteins on viruses against inactivating agents, such as detergents. This protective effect may, however, be eliminated by a higher concentration of Tween or detergent, respectively, without substantially impairing the biologic activity of the proteins. Such a procedure makes it possible to do without the addition of further substances, such as, solvents, whose toxic effect is known.

It has proven to be advantageous if the treatment with Tween or detergent is carried out at a concentration of more than 10 and less than 25 by mass, for a period of time of between 1 min and 30 min, in part 4 cular at a pH of between 5.5 and 8, at

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12 temperatures of between 0°C and 56°C, advantageously between 15°C and 37°C, and optionally at an electric conductivity of from 7 to 20 mS.

A preferred method of producing inactivated blood products according to the invention consists in that the blood product is treated with hot vapor before or after the treatment with the aqueous detergent solution, wherein the blood product is adjusted to a content of water, methanol or ethanol of more than 0.05 by mass) and less than 0.70 (70 by mass), preferably less than 0.40 (40 by mass) in the solid state, and is treated in a closed container at a temperature in the range of from 50 to 121°C.

The invention also relates to a method of determining the virus inactivating capacity of an inactivation treatment comprising at least one inactivation method, by determining the reduction factor by means of a test virus, which is characterised in that the test virus is repeatedly added during the at least one inactivation method, and the individual reduction factors of the at least one inactivation method are optionally added up with the reduction factors of further inactivation methods to give a total virus reduction factor.

The invention will be explained in more detail by the following Examples.

I- -r i 13 Example 1 From human plasma a coagulation-factor-VIIIcontaining cryoprecipitate solution was produced according to a method disclosed in AT-B 391,808. The solution was adjusted to 8 Tween 80 and 7 times admixed with a HIV-1 virus suspension at 2 min intervals. After a total incubation period of 14 min at 0 C, the virus was centrifuged and the titer was determined. The control value of the preparation without Tween addition was 105-1. After the Tween treatment, the virus titer was below the detection limit of 100.5 and could be termed to be 0 based on the negative tests with the reverse transcriptase. This results in a virus reduction factor of 7 x 5.1 35.7.

SThe solution freed from Tween was again admixed *o with HIV-1 virus suspension, lyophilised, and, according to the method of EP-A-0 159 311 heated at 0 C for 10 h at a water content of 8 The virus titer was lowered from 106.2 to 0.

Both inactivation steps thus resulted in a total virus reduction factor of 41.9. Thus, it could be proven that a factor VIII preparation which is subjected to the detergent and heat treatments under the conditions stated above conforms to a total virus reduction factor of 41.9 and can be viewed to be virussafe.

The determination of the residual activity of c rr II ~C q I 14 factor VIII was effected by the aid of the thromboplastin formation test (2 step test). The residual activity of factor VIII was calculated by forming the quotient of the factor VIII activity of the heated sample and the factor VIII activity of the starting material prior to the Tween treatment and amounted to Example 2 A preparation containing the clotting factors II, IX and X (partial prothrombin complex, PPC) was recovered according to the method disclosed in Vox.

Sang. 33, 37-50 (1977) from human plasma by adsorption on DEAE Sephadex, washing of the ionic exchanger and Selution of the complex.

o. The PPC was admixed with HIV-1 virus in a solution containing 22 Tween 80 and incubated at 25"C. The virus suspension was added 15 times at 20 s intervals.

The virus titer of the control without the addition of Tween was 105.7 After the treatment with Tween the Soo final virus titer was below the detection limit of 100.5. From this, a total virus reduction factor of at least 15 x 5.2 78 is calculated. A PPC preparation subjected to the above treatment with Tween thus corresponds to a total virus reduction factor of 78 and is to be viewed as virus-safe.

The activity of the coagulation factors was determined by way of factor IX via the addition of the ~l~asP~ IL--L ~IIPP I 15 sample to be tested to a factor IX deficient plasma and the determination of the activated partial thromboplastin time (1 step test) and was hardly influenced by the treatment with Tween. The ratio of the activity of the treated sample treated to the activity of factor IX in the untreated PPC was approximately 100 Example 3 A PPC preparation as described in Example 2 was incubated at 25 0 C with 12 dimethyl octyl amine-Noxide in the presence of model viruses (Sindbis and vesicular stomatitis virus=VSV). The addition of virus suspension was effected 10 times at 5 min intervals.

After the treatment with detergent, the virus titer was each below the detection limit of 101.5. The control values without detergent addition were 106.4 and 106.1, respectively. From this a total virus reduction factor of at least 10 x 4.9 49 and 10 x 4.6 46 was calculated.

The biological activity was hardly impaired by the detergent treatment and amounted to approximately 100 Example 4 Plasma was fractionated according to Cohn, and the fibrinogen-containing COHN I fraction was admixed with model viruses (Sindbis or VSV, respectively). After lyophilisation, the concentrate having a water content 16 of 8% was heated according to the method of EP-A-0 159 311 for 10 hours at 60 0 C and subsequently for 3 h at 80*C. The virus titer was lowered by lyophilisation from 105.5 and 106, respectively, to 104.9 and 105-5, respectively, and furthermore, by the treatment at 60C to below the detection limit of 100.5 The inactivation capacity of the second treatment step at 80 0 C was determined in parallel preparations: lyophilisation again lowered the virus titer from 105.5 and 106.0 to 104.9 and 1055, respectively, and further on, the treatment at lowered it to below the detection limit.

The reduction factor is calculated from the twotime reduction by 5 or 5.5 log-steps, respectivly, minus 0.6 or 0.5 log-steps, respectively, because during the two-step treatment the fibrinogen preparation was subjected to a single lyophilisation only. The reduction factors thus were 9.4 and 10.5, respectively.

Subsequently, the powder was dissolved in a medium containing 5% octyl glucoside, and at intervals of min Sindbis or VSV, respectively, was added 9 times thereto. After the incubation (a total of 45 min at the virus titer was determined. The treatment with detergent reduced the virus titer to a value of below the detection limit of 100-5. The control value IP~ -~Cbl~~k 17 of a preparation without detergent addition was 106.9 and 106.0, respectively. From this, virus reduction factors of at least 57.6 and 49.5, respectively, were calculated. Thus, the total virus reduction factors were at least 67.0 and 60.0, respectively. A fibrinogen preparation which is subjected to the heat and detergent treatments under the above indicated conditions conforms to a total virus reduction factor of at least 60.0 and is to be considered as virus-safe.

In the precipitate precipitated with 8% ethyl alcohol from the octyl-glucoside-containing fraction, the biologic activity of the fibrinogen was determined by means of a cross-linking test of the fibrin-a-chains Seelich, H. Redl "Theoretische Grundlagen des Fibrinklebers" in K. Schimpf "Fibrinogen, Fibrin und **0 Fibrinkleber", F.K. Schattauer Verlag, Stuttgart-New York, 199-208, 1980) and by means of thrombelastography Hartert in "Thrombosis and Bleeding Disorders", Bang et al., eds.) Georg Thieme Verlag Stuttgart, Acad. Press New York London, 70-76, 1971), coagulation factor XIII having been admixed in each case.

The biologic activity of the treated fibrinogen, based on the biologic activity of the COHN I fraction, was 87 measured by the cross-linking, and 56 measured in the thrombelastogram.

Example Selected plasma was fractionated according to B 1 1 s ~B~n~orm~e a 18 Cohn. The COHN III fraction which contained antitetanus-toxoid gamma globulin was admixed with HIV-1 and Sindbis virus, respectively, in the presence of Triton X-100 and incubated at 25 0 C. Virus addition was effected 30 times at one-minute intervals. After an incubation period of a total of 30 min, the virus titer was below the detection limit of 102.5 and 101-5, respectively. The control values of the preparation without the addition of detergent were 105.7 and 107.5, respectively. From this a total virus reduction factor of at least 30 x 3.2 96 and 30 x 6 180, respectively, is calculated. A gamma globulin preparation which had been subjected to the above detergent treatment corresponds to a total virus i ereduction factor of at least 96 and is to be viewed as S. virus-safe.

The biologic activity was determined with an avidity test. For this, tetanus toxoid was adsorbed on o a microtiter plate, covered with gelatine and washed.

Subsequently, the gamma globulin to be tested was applied to the coated microtiter plate in several dilutions, and non-adsorbed immunoglobulin was washed off. The gamma globulin bound to the tetanus toxoid was .determined by adsorption of an anti human IgG peroxidase conjugate to the Fc portion of the immunoglobulin and furthermore by colour'reaction of the peroxidase with diaminobenzidine and H 2 0 2 and I Ib I=~a 19 subsequent measurement of the optical density.

The avidity of the gamma globulin for the tetanus toxoid was hardly influenced by the treatment with detergent. No significant avidity differences could be detected before and after the treatment.

Example 6 0.95 ml of a solution containing C 1 esterase inhibitor (produced according to Vogelaar E F et al.

(1973) Vox Sang. 26, 118-127 "Contributions to the Optimal Use of Human Blood") were admixed with 20 mg Triton X-100 and incubated at 25°C. To determine the virus inactivation capacity, VSV virus (10il) was repeatedly admixed at intervals of 5 min. When virus had been admixed 5 times, the virus titer was detected to be below "he detection limit of 100.5. The control o value of the preparation without addition of detergent was 107 5 From this, a virus reduction factor of at least 5 x 7 35 is calculated.

The C esterase inhibitor was adsorbed on DEAE 0 Sephadex and washed with 8.89 g/l NaCl solution until .o it was free of detergent. After desorption of the Sinhibitor with 59 g/l NaCl solution, it was dialysed against a buffer containing 1.0 g/l sodium citrate and o 0.4 g/l NaC1 (pH To this solution again VSV virus was admixed before it was lyophilized. The preparation was dry-heated for 24 h at 72 0 C. During lyophilization and the subsequent heat treatment, the virus titer was a -s 20 reduced from 107.2 to below the detection limit of 100-5. The virus reduction factor thus was at least 6.7.

From the virus reduction factors of the detergent and thermal treatments, a total virus reduction factor of at least 41.7 is calculated. The biologic activity of the inhibitor was hardly impaired by the inactivation of the VSV viruses and was approximately 100%.

This application is divided from our co-pending Application No. 18306/92 and the entire disclosure in the complete specification and claims of that application is by cross-reference incorporated into the present specification.

0 0 e ue 0 0 *eo *e

Claims (2)

1. A method of determining the virus inactivation capacity of an inactivation treatment which comprises at least one inactivation method, wherein the reduction factor is determined by means of a test virus, said method comprising the step of adding said test virus repeatedly during the at least one inactivation method and adding up the individual reduction factors of the at least one inactivation method so as to obtain a total virus reduction factor.

2. A method of determining the virus inactivation capacity as set forth in Claim 1, further comprising further inactivation methods, wherein the individual reduction factors of all of said inactivation methods are added up so as to obtain a total virus reduction factor. 00 DATED this 17th day of February 1994 IMMUNO AKTIENGESELLSCHAFT By Its Patent Attorneys: GRIFFITH HACK CO Fellows Institute of Patent a0'as Attorneys of Australia O 0o I l- a II I -a 22 ABSTRACT The invention relates to a method of determining the virus inactivation capacity of an inactivation treatment for blood products which comprises at least one inactivation method, wherein a reduction factor is determined by means of a test virus, said method comprising the step of adding said test virus repeatedly during the at least one inactivation method and adding up the individual reduction factors of the inactivation method(s) so as to obtain a total virus reduction factor. S* 9* 99*