AU656525B2 - Method of preparation of vaccines, and the use of the vaccines thus prepared - Google Patents

Method of preparation of vaccines, and the use of the vaccines thus prepared

Info

Publication number
AU656525B2
AU656525B2 AU78949/91A AU7894991A AU656525B2 AU 656525 B2 AU656525 B2 AU 656525B2 AU 78949/91 A AU78949/91 A AU 78949/91A AU 7894991 A AU7894991 A AU 7894991A AU 656525 B2 AU656525 B2 AU 656525B2
Authority
AU
Australia
Prior art keywords
lytic
gene
vaccines
proteins
partial sequences
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
AU78949/91A
Other versions
AU7894991A (en
Inventor
Werner Lubitz
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EVAX TECHNOLOGIES AG
Original Assignee
EVAX TECHNOLOGIES AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by EVAX TECHNOLOGIES AG filed Critical EVAX TECHNOLOGIES AG
Publication of AU7894991A publication Critical patent/AU7894991A/en
Application granted granted Critical
Publication of AU656525B2 publication Critical patent/AU656525B2/en
Assigned to EVAX TECHNOLOGIES AG reassignment EVAX TECHNOLOGIES AG Alteration of Name(s) in Register under S187 Assignors: LUBITZ, WERNER
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/39—Medicinal preparations containing antigens or antibodies characterised by the immunostimulating additives, e.g. chemical adjuvants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/12—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria
    • C07K16/1203—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-negative bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/52—Bacterial cells; Fungal cells; Protozoal cells
    • A61K2039/523—Bacterial cells; Fungal cells; Protozoal cells expressing foreign proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/555—Medicinal preparations containing antigens or antibodies characterised by a specific combination antigen/adjuvant
    • A61K2039/55588—Adjuvants of undefined constitution
    • A61K2039/55594—Adjuvants of undefined constitution from bacteria
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2795/00—Bacteriophages
    • C12N2795/00011—Details
    • C12N2795/10011—Details dsDNA Bacteriophages
    • C12N2795/10022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Mycology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Virology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Epidemiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Oncology (AREA)
  • Communicable Diseases (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Modified bacteria, obtd. by (1) transforming a Gram negative bacterium with (a) the gene of a lytic membrane protein of a bacteriophage, (b) a lytic toxin-release gene or (c) their partial sequences which code for lytic proteins; (2) culturing the bacteria, (3) expressing the lytic genes and (4) isolation from the culture broth, are used as vaccines or adjuvants. - Pref. during culture, activation of the gene is inhibited or expression is repressed, and lifted only at selected times. The lytic gene is the DNA sequence for the E or L proteins (or their membrane-penetrating domains) or the EL-hybrid protein, or their active partial sequences.
AU78949/91A 1990-07-26 1991-05-24 Method of preparation of vaccines, and the use of the vaccines thus prepared Ceased AU656525B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE4023721 1990-07-26
DE4023721A DE4023721A1 (en) 1990-07-26 1990-07-26 Modified Gram negative bacteria for use as vaccine and adjuvant - are transformed to express lytic protein or toxin, forming ghost(s) which present many cell wall epitope(s)
PCT/EP1991/000967 WO1992001791A1 (en) 1990-07-26 1991-05-24 Method of preparation of vaccines, and the use of the vaccines thus prepared

Publications (2)

Publication Number Publication Date
AU7894991A AU7894991A (en) 1992-02-18
AU656525B2 true AU656525B2 (en) 1995-02-09

Family

ID=6411039

Family Applications (1)

Application Number Title Priority Date Filing Date
AU78949/91A Ceased AU656525B2 (en) 1990-07-26 1991-05-24 Method of preparation of vaccines, and the use of the vaccines thus prepared

Country Status (9)

Country Link
EP (1) EP0540525B1 (en)
JP (1) JP3329451B2 (en)
AT (1) ATE184915T1 (en)
AU (1) AU656525B2 (en)
DE (2) DE4023721A1 (en)
DK (1) DK0540525T3 (en)
IE (1) IE911843A1 (en)
NZ (1) NZ238303A (en)
WO (1) WO1992001791A1 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9918319D0 (en) 1999-08-03 1999-10-06 Smithkline Beecham Biolog Vaccine composition
DE60232071D1 (en) 2001-06-11 2009-06-04 Applied Nanosystems Bv METHOD OF BINDING ACMA-TYPE PROTEIN ANCHOR FUSIONS ON MICRO-ORGANISMS OF CELL WALL MATERIALS
PL399492A1 (en) 2002-08-02 2012-11-19 Glaxosmithkline Biologicals S.A. Vaccine
JP5275982B2 (en) 2006-06-12 2013-08-28 グラクソスミスクライン バイオロジカルズ ソシエテ アノニム vaccine
RU2477145C2 (en) 2008-05-30 2013-03-10 ДЗЕ Ю.Эс.Эй., ЭС РЕПРЕЗЕНТЕД БАЙ ДЗЕ СЕКРЕТАРИ ОФ ДЗЕ АРМИ, ОН БЕХАФ ОФ УОЛТЕР РИД АРМИ Multivalent vaccine of native vesicles of meningococcal outer membrane, method for making and using it
GB201015132D0 (en) 2010-09-10 2010-10-27 Univ Bristol Vaccine composition
KR101825439B1 (en) * 2016-04-15 2018-02-05 배재대학교 산학협력단 Method for preparing Gram positive bacteria ghosts by the treatment with hydrochloric acid

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0291021A2 (en) * 1987-05-12 1988-11-17 Lubitz, Werner, Dr. Recombinant DNA encoding a chimaeric protein with a lytic activity, and its application

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2143238A (en) * 1983-03-31 1985-02-06 Dr Anthony Stuart Breeze A method for enzyme liberation from bacterial cells
US4637980A (en) * 1983-08-09 1987-01-20 Smithkline Beckman Corporation Externalization of products of bacteria
DE4005874A1 (en) * 1990-02-24 1991-11-07 Boehringer Mannheim Gmbh CARRIER-TIED RECOMBINANT PROTEINS, METHOD FOR THE PRODUCTION AND USE AS IMMUNOGENIC AND VACCINE

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0291021A2 (en) * 1987-05-12 1988-11-17 Lubitz, Werner, Dr. Recombinant DNA encoding a chimaeric protein with a lytic activity, and its application

Also Published As

Publication number Publication date
NZ238303A (en) 1993-07-27
JPH06501378A (en) 1994-02-17
EP0540525A1 (en) 1993-05-12
DE59109155D1 (en) 1999-10-28
EP0540525B1 (en) 1999-09-22
JP3329451B2 (en) 2002-09-30
ATE184915T1 (en) 1999-10-15
DE4023721A1 (en) 1992-01-30
DK0540525T3 (en) 2000-04-03
AU7894991A (en) 1992-02-18
WO1992001791A1 (en) 1992-02-06
IE911843A1 (en) 1992-01-29

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