AU651139B2 - Human IFN-beta 2/IL-6, its purification and use - Google Patents

Description

Regulntion 3.2 A US TRA LI A, Patents Act 1952 COMPLETE SPECIFICATION FOR A STANDARD PATENT

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Address for Service: Invention Title: DAVIES COLLISON, Patent Attorneys, 1 Little Collins Street, Melbourne, 3000.

"HUMAN IFN-BETA 2/IL-6, ITS PURIFICATION AN11D

USE"

The following statement is a full description of this invention, including the best method of performing it known to me/us: -1Ir FIELD OF THE INVENTION The present invention relates to human interferon-02 (IFN-P2), to monoclonal antibodies and to hybridoma cell lines producing said monoclonal antibodies and to a method for purification of interferon-F2 employing said monoclonal antibodies. It further relates to pharmaceutical compositions comprising human interfercn-P2, BACKGROUND OF THE INVENTION Human interferon-p2 was first described and cloned from doublestranded RNA-induced human fibroblasts as a 0-type interferon-like *9 activity in U.K. Patent 2063882 of filing date 19.11.80 and the **9o recombinant protein expressed by CHO cells was described in Australian Patent 602872 of 14.10.86, both of the same applicant.

This cytokine has multiple functions and activities and it has been shown to be identical to several proteins described later on in the literature and identified by other biological activities, namely B-cell differentiation or stimulatory factor (BCDF or BSF-2), also named interleukin-6 (IL-6) Hirano et al. (1986) Nature 324, pp. 73-76), hybridoma/plasmacytoma growth factor (HGF or HPGF) Van Damme et al.

(1987) J.Exp.Med. 165, pp. 914-919), hepatocyte stimulatory factor (HSF) Gauldie et al. (1987) Proc.Natl.Acad.Sci.U.S.A. 84, pp. 7251-7255), 26-kDa protein inducible in human fibroblast cells (Haegeman et al. (1986) Eur.J.Biochem. 159, pp. 625-632), and a monocyte-derived human B-cell growth factor that stimulates growth of Epstein-Barr Virus (EBV)transformed human B-cells Tosato et al. (1988) Science 239, pp.

502-504). The protein will be designated hereinafter in this application as IFN-32- IL-6 or IFN-02.

Natural and recombinant IFN-02 produced by mammalian, e.g. CHO cells appear in several forms modified by various glycosylation and phosphorylation processes (A.Zilberstein et al. (1986) EMBO J.5, pp. 259-2537; L.T. May et al.

(1988) J.Biol. Chem. 263, pp. 7760-7768; L.T. May et al. (1988) Biophys. Biochem.

Res. Commun. 152, pp. 1144-1148). Thus immunoblots of denaturing gels may show bands of about 23, 26, 45 and 66 Kd forms. The removal of the carbohydrate moiety of the glycosylated protein yields a smaller form of about 20 Kd which still retains most or all of its biological activity.

SUMMARY OF THE INVENTION The present invention relates to the production of an unglycosylated polypeptide comprising the amino acid sequence of mature human IFN-02, to recombinant vectors comprising a DNA sequence which codes for said polypeptide and microorganisms transformed therewith and to a process for producing said unglycosylated polypeptide by culturing said transformed microorganisms and e see recovering the polypeptide.

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BRIEF DESCRIPTION OF THE DRAWINGS Figure 1 shows the nucleotide sequence and the amino acid sequence of IFN-R2.

Figure 2 shows the construction of plasmid pSVR 2

HB,

Figure 3 shows the construction of plasmids pRIB2802 and pRIB 2 604.

Figure 4 illustrates the analysis of IFN-R2 preparations by Western blotting.

Figure 5 shows silver stain analysis of SDS-PAGE of IFN-P2 eluted fractions.

Figure 6 shows the construction of plasmid pSVR 2 29.

Figure 7 shows the HGF activity of unbound fractions containing IFN-2 .0 produced by CHO clone A2-5-10 after affinity chromatography with monoclonal antibody 34-1.

*p S Figure 8 shows the HGF activity of elution fractions containing IFN-r2 produced by CHO clone A2-5-10 after affinity chromatography with monoclonal antibody 34-1.

Figure 9 illustrates the construction of plasmids pTL2501 and pKKz 2 7.

Figure 10 shows silver stain analysis of SDS-PAGE of E. coli IFN-P2 after immunoaffinity purification and chromatography with S-Sepharose.

Figure 11 shows inhibition of breast carcinoma cell line T47D colony o 9 formation by E. coli IFN- 1 Figure 12 shows results of breast carcinoma T47D end MCF-7 cells clonogenic assay with E. coli IFN-R2.

Figure 13 shows differentiation of myeloleukemic Ml cells induced by E. coli IFN-82.

Figure 14 shows growth and Oligo A synthetase induction in myeloleukemic M1 cells treated by IFN-B2 (in HGF units/ml).

Figure 15 shows effect of IFN-B2 on growth of hematopoietic colonies from normal human bone marrow.

DETAILED DESCRIPTION OF THE INVENTION The anti-IFN-R2 monoclonal antibodies of the invention are produced from a hybridoma cell line obtained by fusion of murine myeloma cells with spleen cells from a mouse previously immunized with IFN-52 or with a fusion protein comprising IFN-2, e.g. Protein A-IFN-52 fusion protein.

According to the invention, there is described the construction of a plasmid for the expression in E. coli of a Protein A-IFN-32 fusion protein and its use for obtaining monoclonal antibodies. The complete translated sequence of the cDNA coding for human IFN-2 of Fig. 1 was fused, in phase, to the 3' end of the coding sequence for the staphylococcal Protein A affinity tail (Uhlen et al. (1984) J.Biol.Chem. 259, p. 1695). For efficient expression in E. col> the hybrid gene was fused to the strong lambda PR promoter.

6@ The resulting Protein A-IFN-2 fusion protein was purified and used to immunize mice. After six injections of the purified protein into mice, positive sera were tested for their binding titer in a solid phase radioimmunoassay (SRIA) and for the specificity of binding by Western blots.

Spleen cells derived from a mouse showing the highest Linding titer g (dilution 1:25,000) were fused to mouse myeloma cells. The fusion of the cells is done in the presence of a suitable fusion promoter of those known in the art. The fused cells are then cultured in separate wells. The oe0o supernatant of each well is then tested for the presence of the desired monoclonal antibody capable of specifically binding to IFN-52, preferably with IFN-02 from a different source than the IFN-a2 used for the immunization of the mice. Thus, if a Protein A-IFN-02 fusion protein expressed by E. coli cells is used to immunize the mice, then the screening of the monoclonal antibodies is performed with IFN- 2 produced by CHO cells. This prevents cross-reaction of the Protein A and of any E. coli contaminants in the antigen preparations used for injection with some of the monoclonal antibodies during the =creening. For the SRIA, crude supernatants of CHO cells, harboring a plasmid containing the human IFN-02 gene under the control of the SV40 early promoter and expressing high levels of this gene but no Protein A or any bacterial antigen, were bound to a solid support and reacted with supernatants of the hybridomas and with [x251] goat antimouse antibodies.

The hybridomas were screened by the SRIA and several positive clones were isolated and characterized. The positive clones producing the desired antibodies are then selected and subcloned and either cultured in a suitable growth medium or injected into mice, and the desired monoclonal S. antibodies are then recovered from the supernatant of the cultured cells or from the ascitic fluid of said mice, respectively.

The monoclonal antibody obtained is bound to a solid phase support contained within a column. Any suitable gel matrix known in the art may be used to immobilize the monoclonal antibody e.g. agarose-polyacrylhydrazide. Crude preparations of human IFN-R2 preparations are loaded on the column and IFN-P2 is eluted from the gel by a change in pH or ionic 1 strength. In a preferred embodiment, the fractions containing IFN-P2 are eluted with a buffer pH 2 and neutralized immediately after elution with a buffer pH The crude natural IFN-f2 preparations purified according to the invention are obtained from induced fibroblast cells together with IFN-Rl.

The recombinant IFN-R2 produced by CHO cells to be purified is obtained according to the process described in A. Zilberstein et al.

(1986) EMBO J. 5, pp. 2529-2537.

According to the present invention, an unglycosylated polypeptide comprising the amino acid sequence of IFN-82 is obtained by recombinant DNA techniques. Human DNA, particularly cDNA coding for a polypeptide comprising the amino acid sequence for IFN-P2, is fused through the coding region to a strong bacterial promoter, such as hybrid tryp-lac promoter, and this fused DNA molecule is inserted into a suitable plasmid so as to obtain a recombinant vector comprising said DNA sequence and regulatory regions which are positioned in such a way that expression of said polypeptide in bacterial cells is possible. Bacterial cells, e.g. E. coli, are transformed by said recombinant plasmids and cultured in order to express the desired polypeptide, which is subsequently recovered and purified.

The IFN-P2 molecule as described is a polypeptide comprising 212 amino acids. At the N-terminus of the protein may be found the sequences Ala 28 -Pro-Val-Pro-Pro- or -Pro 29 -Val-Pro-Pro- or -Val 30 -Pro-Pro- of Fig.

1. In our preferred embodiment, the IFN-R2 cDNA was fused at the Pro 29 codon to a Met initiator codon and a tryp-lac promoter, but this and all other sequences are covered by the present invention as long as the polypeptide has IFN-B2/IL-6 activity.

The DNA vectors used in this invention were constructed by standard procedures. Plasmid DNAs were purified by banding in CsCl-ethidium bromide gradients. DNA restriction fragments separated by electrophoresis in agarose or polyacrylamide gels were purified on DE-52 columns. Restriction endonucleases (Boehringer, New England Biolabs), T4 DNA ligase (New England Biolabs), the large fragment of E. coli DNA polymerase (Boehringer) and T4 polynucleotide kinase (Pharmacia), were used as

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recommended by the suppliers. E. coli transformation was carried out with frozen competent bacteria Morrison (1979) Methods Enzymol. 79, pp.

326-331) using strains HB101 ATCC 33694, JM101 ATCC 33876, N4830-1 (Gootesman et al. (1980) J.Mol.Biol. 140, p. 57) and JM105 (Messing et al.

(1981) Nucleic Acids Res. 9, pp. 309-321).

The present invention discloses the hematopoietic effect of IFN-R2 and its use in the inhibition of breast carcinoma cell growth, in the growth inhibition and differentiation of myeloleukemic cells and in the induction of Complement Factor B in fibroblasts. Thus human IFN-52 is used as active ingredient of pharmaceutical compositions for the treatment of breast cancer, leukemia, infectious diseases and bone marrow progenitor cell disorders.

The invention will now be illustrated by the following examples, without delimiting its scope: Example 1. Preparation of Protein A-IFN-B2 fusion protein A) Construction of plasmids pRIz2802 and pRIt 2 604 Plasmid pSV 2 HB (Fig. 2) is one of the vectors used for constitutive 4g.

expression of the IFN-P2 gene in CHO cells under the strong SV40 early promoter and is derived from plasmid pSVCIFB Zilberstein et al, a9 (1986) EMBO J. 5, pp. 2529-2537) by removing all the 5' and 3' non-coding a a sequences of the cDNA by standard cloning techniques.

The 661 bp CDNA insert coding for IFN-R2 was excised from plasmid pSVRaHB as a 661 bp Hind III/Cla I fragment and digested with Eco RII. The resulting five fragments were separated on a preparative agarose gel. The three small fragments of 55, 12 and 37 bp coding for the signal peptide sequence and for the first three amino acids of the mature protein were discarded and the two fragments of 239 a, 318 bp were recovered from the gel. In order to restore the sequence coding for the first amino acids and to maintain the Protein A frame, a double stranded synthetic oligonucleotide was prepared (sequence shown in Figure 3) and ligated together with the 239 bp and 318 bp fragments into plasmid pGEM-1 previously digested with Eco RI and Acc I. The resulting plasmid was called pf2132 (Fig. 3) and contains the whole IFN-P2 sequence preceded by an asparagine and a serine codon within the multiple coding site of plasmid pGEM-1. The asparagine and serine codons are the two codons at the unique Eco RI site (at the 3' end of the Protein A gene) of plasmid pRIT2T (Pharmacia) that was used for subsequent cloning. Plasmid pR213 2 was digested with Eco RI and Hind III and the complete IFN-P2 cDNA sequence was isolated and introduced into plasmid pRIT2T digested either with Eco RI and Pvu II or with Eco RI and Sma I restriction endonucleases for the obtention of the plasmids pRIR2802 and pRIR 2 604, respectively (Fig. 3).

B) Production of Protein A-IFN-52 fusion protein and its purification Strain E. coli N4830-1 (Gootesman et al. (1980) J.Mol.Biol. 140, p.

57) was transformed with recombinant plasmids pRIR2802 and pRIp2604 giving origin to new microorganisms E. coli N4830-l/pRa2802 and E. coli N4830-1/ pRB2604, respectively.

Diluted cell cultures of the microorganisms were grown overnight at 30 0 C in M9 medium containing ampicillin to early stationary phase, then *9 incubated at 42*C for 90 minutes, cooled and harvested by centrifugation.

0After repeated resuspension and centrifugation, 20% SDS was added to a final concentration of 1% and 10M urea to a final concentration of 8M, and the extract containing the expressed Protein A-IFN-2 fusion protein was dialyzed against TST (50 mM Tris pH 7,6, 150 mM NaCI and 0.05% Tween 0* The clear supernatant after dialysis was applied to the IgG Sepharose 6 Fast Flow (FF) equilibrated column. After loading on the column the gel was washed and the bound fusion protein was eluted with 0.5 M NH 4 COOH, pH 3.4 and lyophilized directly without prior dialysis.

Example 2. Preparation of anti-IFN-82 monoclonal antibodies A) Immunization of mice and cell fusion Three-month old female Balb/c mice were first injected with the partially purified Protein A IFN-R2 fusion protein obtained in Example 1 above (10g/mouse, emulsified in complete Freund's adjuvant). Three weeks later the mice were given a subcutaneous boost with the fusion protein in solution. Four additional injections were given at 10 days intervals. The mouse showing the highest binding titer (Table 1) and the strongest signal in Western blot analysis received an intra- peritoneal injection of the fusion protein and three days later its splenic lymphocytes (150 x 106 cells) were fused with 30 x 106 NSO/I myeloma cell line. The fused cells were distributed into microculture plates (3 x 104 cells/well) and selected for hybridoma growth. Hybridomas that were found to secrete anti-IFN-82 antibodies were cloned and recloned by the limiting dilution technique.

SB) Screening for anti'IFN-2 monoclonal antibody-producing specific e hybridomas Hybridoma supernatants were tested for the presence of anti-IFN-2 S antibodies by a solid phase radioimmunoassay (SRIA). PVC microtiter plates (Dynatech Laboratories, Alexandria, VA) were coated with a crude, serum-free supernatant of CHO cells secreting IFN-R2 Following an incubation of 2 hrs at 37'C or 16 hrs at 4'C the plates were washed twice with PBS containing BSA and Tween 20 and d« blocked in washing solution for hrs at 37*C. Hybridoma culture supernatants (50 ig/well) were added and the plates were incubated tir 4 hrs at 37'C. The plates were then washed three times with the washing solution and x 25 s-goat anti-mouse (Fab')a (50jl, 10 s cpm) was added for S further incubation of 16 hrs at 4'C- The plates were washed 4 times and individual wells were cut and counted in a gamma counter. Samples giving counts that were at least four times higher than the negative control value were considered positive (Table 1).

Table 1: Screening of hybridomas by SRIA Sample dilution cpm Immune serum (mouse) 1:4000 2800 negative control (mouse) 1:4000 100 Hybridoma 12 4000 12 1:125 300 27 1100 28 200 34 6200 34-1 1:2500 1000 38 2600 48 1500 102 1200 117 1400 123 1100 125 1600 132 5400 136 2700 154 1500 157 2400 negative hybridoma 200 Ascitic fluid 1:62,000 1400 Ascitic fluid negative 1:12,000 300 0 As can be seen from Table 1, fourteen anti-IFN-R2 hybridomas were

V.

selected using the SRIA. Hybridoma No. 34-1, sub-cloned from hybridoma 34, was further charaLterized and was found to belong to IgGI class.

Hybridoma 34-1 was deposited with the Collection Nationale des Cultures de Microorganismes CNCM, Institute Pasteur, Paris, on 14.11.88. It was accorded No. 1-813.

Hybridoma 34-1 was found suitable for Western blotting and for affinity purification of natural and recombinant IFN-2 expressed both by E. Coli and by CHO cells. It was used in the following experiments.

9 Example 3. Applications of the anti-IFN-32 monoclonal antibodies A) Western blotting Samples of crude preparations of either natural or recombinant IFN-02 expressed by CHO and E. coli cells were analyzed by SDS-PAGE under reducing conditions and electroblotted onto nitrocellulose sheets Schleicher and Shuell). Following electroblotting the sheet was incubated overnight with a blocking buffer non-fat milk in PBS containing 0.05% Tween 20 and 0.02% sodium azide) and then for 2 hrs at room temperature with the anti-IFN-52 antibody No. Following washing in 0.05% Tween 20 in PBS, the nitrocellulose was incubated for 3 hrs at room temperature with x 25 I-goat anti-mouse serum (0.7 x 106 cpm/ml in the blocking buffer). The sheet was then washed, dried and autoradiographed.

The results are shown in Fig. 4: Lane A: natural IFN-B2; Lane B: recombinant IFN-2 of CHO cells; Lane C: recombinant IFN-52 of E. coli cells; Lane D: recombinant IFN-a1 of CHO cells (comparison).

B) Affinity chromatography of IFN-82 preparations Ascitic fluids of mice containing monoclonal antibodies secreted by hybridoma 34-1 were precipitated with ammonium sulfate (50% saturation) 16 *hrs at 4°C. The precipitate was collected by centrifugation, redisolved in water and dialysed against saline. About 10 mg of immunoglobulins were bounl to 1 ml agarose-polyaeryl-hydrazide according to Wilcheck and Miron Methods Enzym. 34, p.72). Crude preparations of either natural (fibroblast) or recombinant coli or CHO) IFN-R2 (containing 0.5 M NaCI) were loaded at 4°C at a flow rate of 0.25 ml/min. The column was S* washed with 30 column volumes of 0.5 M NaCI in PBS. IFN-62 was eluted by 50 mM citric acid buffer, pH 2 (8 x 1 column volume fractionsj and see** immediately neutralized by 0.1 M Hepes buffer, pH Crude recombinant IFN-52 coli extract depleted from DNA) was Se* loaded on 1 ml of the anti-IFN-32 column. Purification of 1000 fold was does achieved in one step, and the recovery of IFN-P2 was 100% (Table The procedure was scaled up using 8 ml affinity column. The capacity of the column was 400 pig pure IFN-B2 per 1 ml of column. Silver stain analysis of SDS-PAGE of the eluted fractions revealed a major band of a M.W. of 21,000 and some minor contaminants of a higher M.W. (Fic. When crude recombinant IFN-82 (CHO) was loaded on 1 ml affinity column, purification was achieved in one step with a recovery of 100%. Silver stain analysis of SDS-PAGE of the eluted fractions revealed two major bands VZ 23 kDa and 28 kDa, both belonging to the glycosylated forms of IFN-P2 (Fig. The same bands were obtained when natural IFN-P2 (foreskin fibroblasts) was immunoaffinity purified.

Table 2 Source Sample HGF Prot.conc. Spec.act. Purif. Recovery of IFN-B2 units/ml mg/ml units/mg fold t x 10- 4 E. coli load 1.4 4.1 3400 efluent 0.54 4.1 1300 eluate 25 0.09 3.6 x 106 1060 100 CHO load 0.2 efluent 0.06 eluate 2.7 0.14 0.2 x 106 100 Foreskin load 0.014 1.46 fibroblast efluent 0.014 eluate 0.014 0.008 17 x 104 180 100 so Example 4. Monitoring of IFN-a2 produced by CHO cells t IFN-B2 produced and secreted in one liter of culture medium by CHO clones was quantitated using monoclonal antibody 34-1 for purification by immunoaffinity.

Clone A2-5-10 is a CHO clone obtained by transfection of CHO cells with plasmid pSVp 2 29 (Fig. 6) and selection with 50 nM methotrexate (MTX).

G Plasmid pSVRa29 was obtained as follows: a DNA fragment containing the sequence coding for IFN-R2 fused to the early promoter of SV40 and to the SV40 polyadenilation site was excised from plasmid pSVcl5 as a 2.5 kb o; BamHI fragment. Plasmid pSVcl5, one of the vectors previously used for constitutive expression of IFN-82 in CHO cells under the strong SV40 early promoter, was derived from plasmid pSVCIF0 2 Zilberstein et al. (1986) EMBO J. 5, pp. 2529-2537) by removing all the sequence 5' to the XhoI site of the IFN-02 cDNA. This BamHI fragment was cloned into the BamHI site of a pDHFR plasmid containing a mouse DHFE cDNA fused to the SV40 early promoter and a splicing region of mouse IgG gamma-2a.

The clone A2-5-10 was grown to confluency in roller bottles. The culture medium war changed to a low fetal calf serum and collected 24 hours after the change. One liter of culture was concentrated to 45 ml and loaded on the monoclonal antibody affinity column prepared in Example 3B. The column was extensively washed and the bound IFN-R2 was eluted with mM citric acid pH 2, in four fractions of one ml each. The IFN-R2 purified in this way seems to be homogeneously pure as indicated by .ilver stain analysis of SDS-PAGE (Figure The amount of IFN-R2 protein recovered from one liter of culture was 6F9 ug.

The amount of IFN-R2 in each fraction was estimated by measuring the hybridoma growth factor (HGF) activity of the protein in the crude preparation and in the different fractions of the affinity column. About 40% of the IFN-R2 loaded on the column was recovered in the unbound fraction (Figure while the remaining activity was recovered in fractions eluted with pH 2 (Fig e 8) with a peak in elution 2. These results indicate that, under the conditions described above, clone A2-5-10 produces about 800 pg/l of IFN-R2.

0 The specific activity of the IFN-52 produced and secreted by the CHO 6 clone A2-5-10 was determined by measuring the HGF activity and the protein concentration in each of the purified fractions of the immunoaffinity column. One unit of HGF is defined as the amount of protein that gives 50% of the maximal effect in the assay. The HGF activity was assayed in 0.1 ml cultures of murine plasmacytoma T1165 cells, treated for 24 hours and pulsed for 16 hours with [(H]thymidine as described by Nordan R.P. and Potter M. (1986) Science 233, pp. 566-568. Table 3 summarizes the results of such an analysis. The specific HGF activity of IFN-R2 in the three fractions, eluted from the affinity chromatography column, ranged from 1.18x106 to 2.1x106 with an average of 1.47x10 6 Table 3 Specific Activity of IFN-82 Fraction HGF Protein Specific activity concentration activity U/ml mg/ml U/ml Elution 1 128,000 0.108 1.18x106 Elution 2 333,000 0.239 1.39x10 6 Elution 3 166,000 0.079 2.10x10 6 Total 627,000 0.426 1.47x106 The elutiot, fractions from the affinity chromatography column were pooled, dialyzed to 10mM acetate buffer pH 5 and loaded on a Mono S cation exchange column (Pharmacia). The column was washed with 10 mM acetate e* buffer pH 5 and then eluted using a linear sodium chloride gradient from 0 to 600 mM. HGF activity was determined in the different fractions.

Activity coincided with the main peak of protein.

6 Example 5. Preparation and purification of recombinant IFN-02 produced by E. coli cells A) Construction of plasmids pTLz 2 501 and pKK 2 7 Plasmid p12324 containing the whole IFN-P2 sequence preceded by an ATG

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codon within the multiple coding site of plasmid pGEM-1 was prepared in the same way as plasmid p3z132 in Example 1A and Figure 3, except for S the fact that a different synthetic oligonucleotide (Fig. 9) was used.

Plasmid pD2 3 2 4 was digested with Eco RI and Hind III and the complete IFN-R2 cDNA sequence was isolated and introduced into either one of plasmids pTLal43-4 Chernajovsky et al., (1983) Ann. N.Y. Acad. Sci.

413, pp. 88-96) or pKK223-3 (Pharmacia) digested with Eco RI and Hind III for the obtention of the plasmids pTL 2 501 and pKKa7 (Fig. 9).

B) Expression of the biologically active IFN-2 polypeptide in E. coli Strain E. coli JM105 (described in J. Messing et al., (1981) Nucleic Acids Res. 9, pp. 309-321) was transformed with recombinant plasmid pKKB27 giving origin to new microorganism E. coli JM105/pKK 2 7 deposited with the ATCC under the Budapest Treaty on 17.12.1987 and assigned the number ATCC 67583. The transformation of strain E. coli JM101 (ATCC 33876) with the recombinant plasmid pTLz 2 501 gave origin to new microorganism E. coli JM101/pTLB 2 501 deposited with the ATCC under the Budapest Treaty on 17.12.1987 and assigned the number ATCC 67584. The microorganisms were cultured, lysed and the extracts containing IFN-02 were purified.

C) Purification of E. coli IFN-f2 by chromatography Nucleic acid-free bacterial extracts were obtained by polyethyleneimine precipitation and passage on DEAE cellulose. The effluent fractions were adsorbed onto S-Sepharose in 10 mM Na acetate buffer pH 5 (buffer A) Sand eluted with a 0-0.03 M NaCI gradient. Active fractions were pooled, dialyzed against buffer A, chromatographed on a Mono-S FPLC column and eluted with a 0-0.03 M NaCl gradient. HGF activity was followed during purification and coincided with the IFN-P2 protein revealed by immunoblots using polyclonal antibodies against a N-terminus peptide of IFN-R2. The final preparation showed a single band of about 20 kDa on SDS-polyacrylamide gel electrophoresis.

9, D) Immunopurification of E. coli IFN-i2 For purification, 1-1.5 1 of Dyno-mill extract obtained from 3-6 S liter of fermentor culture were precipitated with polyethyleneimine, the solution was concentrated down to 100 ml (A4) or 500 ml (A8) and loaded on o 9 an 8 ml column of monoclonal antibody 34-1 (Ig from ascites, 8 mg Ig/ml column) in phosphate buffered saline (PBS) with 0.5M NaCI pH 7.0 (A4) or 1M NaCI After washing the column with the same buffer, elution was carried out by 50mM citric acid buffer pH 2 (A4) or with the same citric buffer and propylene glycol 25% (A8) and samples were immediately neutralized by addition of 0.1M Hepes buffer pH8.5. The results were as follows: A4: Immunopurification of E. coli IFN-02: Fraction Volume Protein HGF activity Load 90 ml 5,700 mg 29 million U eluted: Total 40 ml 8.2 mg 2.9 million U (Tube 3) 10 ml 2.2 mg HGF U/ml 325,000 110,000 HGF U/mg 5,100 500,000 A8: Immunopurification of E. coli Fraction Volume Protein Load 350 ml 2,550 mg eluted: Total 37 ml 5.2 mg (Tube 3) 9.5 ml 2 mg IFN-32: HGF activity 10 million U 22 million U HGF U/ml 330,000 1.3 million HGF U/mg 4,100 4,2 million *5U

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94 9 *0 09 0 09 *9 9 0*90 9 0 9.

Following immunoaffinity, an S-Sepharose column was used as the final purification step. In a typical experiment, (S12), the input from pooled A4 fractions was dialyzed against 10mM acetate buffer pH 5, adsorbed and eluted by a gradient from 0.1-0.4 M NaCl. The peak eluted at 0.3 M. The results were as follows: S12: S-Sepharose after immunoaffinity Fraction Volume Protein HGF activity HGF U/ml Input: A4 pool 17.5 ml 3.6 mg 1.4 million U dial. 22.5 ml 2.2 mg ND Eluted: Total peak 4.5 ml 0.6 mg 0.9 million U Tube 58 0.5 ml 0.04mg 210,000 HGF U/mg 0.5 million 2 million 5.2 million The yield in this step was 64%. The product was run on SDS polyacrylamide gel under reducing and non-reducing conditions and showed one single band at 21 Kd. (Fig. Example 6. Growth inhibition of breast carcinoma cells While IFN-P2/IL-6 stimulates growth of plasmacytoma/hybridoma cells (HGF activity), it is growth inhibitory on other cell types. We have studied colony formation by the T47D line of human Breast Ductal carcinoma cells (ATCC HTB 133), which is poorly inhibited by IFN-81 but is sensitive to inhibition by IFN-R2. Fig. 11 shows inhibition of T47D colony formation in culture dishes by pure E. coli rIFN-2/IL-6. The 50% inhibition is seen with 10-20 HGF U/ml. At doses over 100 U/ml, the remnant colonies are very small and appear to represent mainly growth arrested cells (insert).

Inhibition of 3 H-thymidine incorporation in semiconfluent T47D cells by E. coli and CHO rIFN-R2/IL-6 was found to be neutralized by the anti-IFN-52 monoclonal antibody 34-1.

To investigate if the inhibition of 3 H-thymidine incorporation in T47D cells represents a genuine antigrowth activity, we used a 15 day clonogenic assay (Fig. 12). A 50% decrease in the number of colonies of T47D cells was observed with 2 BSF-2 U/ml of E. coli IFN-R 2 and an almost complete inhibition of growth in these conditions was reached at 50 U/ml.

The mock preparation had no inhibitory activity in this clonogenic assay I (Fig. 12 In the same experiment we observed that IFN-Px at 500 antiviral U/ml produced no inhibition of T47D cell growth. Decreased colony formation was similarly observed in another breast carcinoma cell line MCF-7 (ATCC HTB 22) (Fig. 12 By 3 H-thymidine incorporation, MCF-7 appears somewhat less sensitive than T47D cells.

For the clonogenic assay, T47D cells were seeded at 200 cells per well and MCF-7 at 1,000 cells per well in 6-well Costar plates in 1 ml RPMI 1640 with 10% fetal calf serum (FCS), insulin 0.2 U/ml, glutamine 2 mM, penicillin 100 U/ml, streptomycin 100 ug/ml. Cells received 24 hours later serial 5 fold dilutions of E. coli IFN-a2 or mock E. coli preparations at the same protein concentration. After 15 days the colonies were stained with crystal violet and counted under an inverted microscope. For DNA synthesis measurements, cells were seeded at 15-25 x 103 per well of a 96-well microplate, and after 3 days FCS was removed for 24 hours and readded in fresh medium with serial 5 fold dilutions of E. coli IFN-R2 or mock preparations. After 16-24 hours, cells were labeled with 15 Ci/ml 3 H-thymidine (25 Ci/mmol, Amersham) for 1 hour, washed twice with PBS, treated by 5% trichloroacetic acid (TCA) for 30 min at 4°C and washed 3 times with 5% TCA. The precipitate was dissolved in 0.1 ml of 0.2 M NaOH at 37°C for 30 minutes, neutralized by 0.01 ;nl of 2M HCl and counted in a Tricarb counter with toluene scintillator and Lumax (3:2 Similar results were obtained without serum starvation, S, 3H-thymidine incorporation was lower but inhibition was the same.

Extracts containing IFN-R2 were assayed for BSF-2 activity as measured by the stimulation of IgG secretion by CESS cells in response to treatment with said extracts Muraguchi et al., (1981) J. Immunol. 128, pp. 1296-1301; T. Hirano et al., (1985) Proc. Natl. Acad. Sci. USA' pp. 5490-5494) and for HGF activity as measured by the ability of the extracts to support the growth of plasmacytoma cell line T1165 (R.P.

Nordan and M. Potter, (1986) Science 233, pp. 566-569). Stimulation of 3 H-thymidine incorporation in T1165 cells and of IgG secretion by CESS cells showed half-maximum at a dilution of 1:12,500 which was therefore defined as one unit of BSF-2/HGF activity. This unitage is used in the present experiments.

Example 7. Growth inhibition and differentiation of mycloleukemic cells IFN-R2 is also active in growth inhibition and differentiation of myeloleukemic cells. Murine myeloleukemic MI cells and human histiocytic lymphoma U937 cells were grown in RPMi 1640 with 10% fetal calf serum (FCS). The cells were seeded at 10 s per ml in wells of 12-well Costar plates. Pure E. coli IFN-R2 was added at 0,1-75 ng/ml and the cultures were observed for 4-6 days. Cells were counted and stained for Giemsa and for non-specific esterase using the a-naphtyl acetate esterase kit 91-A of Sigma (St. Louis, MO). Lysozyme activity was measured in 0.5% Triton-Xl00 cell extracts by a turbimetric assay of the lysis of Micrococcus lysodeikticus (Sigma the assay being calibrated with egg-white lysozyme as described (Weisinger, G. and Sachs, L. (1983) EMBO J. 3, pp.

2103-2107). The oligo-A synthetase activity was assayed in cell extracts as described (Resnitzky et al. (1986) Cell 46, pp. 31-40).

Table 4 0 1 2.3 19.0 25.0 35.0 1 2.0 3.5 0.9 0.7 1 1.4 2.3 0.8 1.2

OS

R Recombinant E. coli IFN-R2 purified by Mono-S FPLC (Example 5C) ,•oEf fWithout addition, the M1 cells grew without adhering to the dish and showed typical myeloblastic morphology. In contrast, after 4 days of cultre with IFN-, th e ells were adherent and showed dramatic morphological changes (Figure 13). About 60% of the cells acquired macrophage-like morphology, the rest showing various degrees of maturation. Cytoplasms were enlarged, contained vacuo (Examples and -quired typica l foamy appearance. Nuclei were eccentric, less round and contrasted and had less prominent nucleoli. Viable cell counting showed culture with IFN-R2, the cells were adherent and showed dramatic macrophage-like morphology, the rest showing various degrees of typical foamy appearance. Nuclei were eccentric, less ound and contrasted and had less prominent nucleoli. Viable cell counting showed that while the control culture grew for 6 days, the Ml cells treated by U/ml IFN-R2 underwent 2-3 divisions and growth was arrested (Table 4).

At day 4 after seeding, less than 1 U/ml IFN-a2 (expressed in plasmacytoma growth units) was sufficient to cause a 50% decrease in Ml cell number. The growth-arrest effect was maximal above 30U/ml IFN-R2 ng/ml). Even with the chemically purified rIFN-a2, this concentration corresponds to no more than 2.5 pg/ml LPS which had no effect on the Ml cells. Crowth inhibition and differentiation of Ml cells was observed when IFN-52 was added with 5 ig/ml polymyxin B, further excluding any role of LPS traces. As a biochemical marker of differentiation we measured lysozyme activity in extracts of 5 x 100 M1 cells cultured 4 days with U/ml IFN-R2. Lysozyme was undetectable in the control M1 cultures.

Treatment with IFN-B2 induced lysozyme to levels of 0.85 ag lysozym.e equivalent per 5 x 106 cells. Phagocytic activity on latex beads was also observed in the differentiated M1 cells.

In another experiment, addition of IFN-P2/IL-6 to cultures of M1 cells arrested the growth of the cells after 24 hours (Fig. 14) and induced their differentiation into macrophages. At 24 hours, tie cells already showed cytoplasmic enlargement with acentric nuclei and after 3-4 G* days acquired typical macrophage morphology demonstrated by increase in G St. lysozyme phagocytotic activity and increase in Mac 1 antigen. The growth inhibition of M1 cells was observed with about 0.5 ng/ml of rIFN-f2/IL-6, less than what is required for stimulation of plasmacytoma T1165 cells. The effect of IFN-P2/IL-6 was more rapid than that of the combination of IL-1 (10 U/ml) and TNF (10 3 U/ml) which produced growth arrest only after 48 hours. These cytokines which also cause Ml differentiation are known indu"x, of IFN-02/IL-6. The growth-arrest by IFN-a2/IL-6 was fully neutraliz' ,y monoclonal antibody 34.

Table Effect of IFN-B2/IL-6 on histiocytic lymphoma U937 cell growth and differentiation Expt IFN-52 Cell number Esterase positive BSF U/ml x 10- 5 Cells, per cent 1. 0 14.0 (100).0 4 100 10.0 71) 24 2. 0 26.7 (100) N.D.

150 23.5 88) 1500 14.5 54) Cells treated for 5 days with or without rIFN-p2 purified on Mono-S FPLC. (Example Human histiocytic lymphoma U937 cells can be induced to differentiate by phorbol esters and Vitamin D3, partially by IFN-gamma and other yet unidentified cytokines. We examined the effect of 100 HGF U/ml IFN-a2 addition on U937 cultures. After 4-5 days, about 25% of the cells showed monocytic/macrophage morphology and there was a 30% reduction in cell growth (Table The cells were stained for a-naphtyl acetate esterase as a biochemical marker of differentiation not induced by IFN-gamma. About a fourth of the cells in the IFN-52 treated culture were strongly positive for the non-specific esterase, whereas few positive cells were observed in 4 a o* the non-treated culture (Table 5) or in mock treated culture (not shown).

With higher amounts of the pure rIFN-P2 preparations, gr. :th inhibition 0 0 (Table 5) and partial morphological changes, such as cytoplasmic 0 enlargement and nucleus indentation, were more pronuanced. However, we found that when added together with IFN-gamma, the effect of low dose TFN-32 was significantly potentiated (Table Under these conditions, cell growth was reduced and most of the cells showed cytoplasmic enlargement, changes in nuclear shape and nucleoli reduction, although monocytic differentiation was still incomplete. Thus we found that the combination of IFN-gamma 100 U/ml and IFN-a2 (1-10 HGF U/ml) has a synergistic effect and triggers growth arrest and differentiation. In optimal conditions, IFN-gamma alone reduced growth (after 6 days) by IFN-P2 alone by 25% and the combination IFN-gamma and IFN-R2 by Table 6 Synergistic effects of IFN-32/IL-6 and IFN-gamma on histiocytic lymphoma U937 cells IFN-52 IFNgamma Cell number A Synthetase Activity BSF U/ml U/ml x 10-5 32 P-A2'pA, cpm 0 0 20 (100) 110 0 14 70) 310 0 100 12 60) 940 100 8.5 42) 4,000 Cells treated for 6 days with or without rIFN-52 immunoaffinity purified.

a* The additien of IFN-gamma also strongly potentiated the induction of *4 Oligo A synthetase by IFN-B2 (Table 6) suggesting that the two cytokines cooperate to initiate the differentiation process, although other additions may be required to see complete differentiation of the type seen with the Ml cells.

IFN- 2 activity on fresh leukemic cells of acute myelogenous leukemia (AML) was also studied. Peripheral blood mononuclear cells from AML patients incubated 5 days with rIFN-02/IL-6 showed a decrease in the percentage of blast cells (from 20-30% in control cultures to 6-12% with OI IFN-P2/IL-6) with an increase in myelocytic forms at various stages of differentiation and in the ratio of myelomonocytes to blast cells. The results with two AML patients are shown in Table 7. GM'CSF was also tested (comparison). IFN-f2/IL-6, therefore acts also on fresh leukemic cells and such tests may be useful to foresee the therapeutic value of the cytokine in AML.

Table 7 Effect of IF, .2/IL-6 on blood cells from AML patients Patient #I 1 Percent Ratio: Blasts Myelomonocytes Blast cells Patient D 2 Percent Ratio: Blasts Myelomonocytes Blast cells 9 a a..

A

a

S

*5 a a a at a aS *4 5 00* S 9 49 A A .4 After 5 days of culture with: FCS ALONE 30 2.3 20 3.6 +GM-CSF 39 1.6 40 1.4 +IFN-a2 (CH017) 11 8.1 4 22.5 +IFN-R2 (E.COLI) 15 5.3 7 12.4 Example 8. Hematopoietic effects on normal bone marrow cultures Monocyte and TI-cell depleted human bone marrow cells treated by 4-hydroperoxy cyclophosphamide (4-HC) (100jig/ml, 30 min.) to eliminate committed progenitors of CFU-mix (colony-forming unit granulocyte, monocyte, erythroid, megakaryocytes), CFU-GM (colony-forming unit granulocyte, monocyte) and BFU-E (burst-forming unit erythroid) colonies, were used to study the effec~ts of rIFN-f32/IL-6 on the early steps in hematopoietic differentiation. When added at the time cells were plated on methylcellulose (105 cells/ml), IFN-132/IL-6 by itself could not support the growth of colonies, indicating it does not function as a growth-promoting CSF (colony stimulating factor) (Fig, 15), However, IFN-a2/XL-6 markedly increased the ability of IL-3 to cause formation of colonies with mixed (CFU-GEM4) aud erythroid (BFU-E) as well as granulocytic monocytic (CGU-GM) phenotypes. In this action IFN-f32/IL-6 appears more potent than IL-1 (Fig, 15). In a two-stage assay, where IFN-f32/IL-6 Wass added in liquid culturas one week before the cells were plated in methylcellulose with a full supplement of CSF, an increase in the number of progenitor cells able to respond to CSF was produced by IFN-f32/IL-6 alone (Fig. 15, right). This increase was only slightly lower than that caused by IL-3 and the two factors seem to work independently in a hue a. ~q a kg 0 ha..

9 4

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98659 a at U C a ass.

a teSSS a a a this first stage of the assay. In Fig. 15, colonies were counted after days and classified as CFU-mix, CFU-GM and BFU-E Left half: Day 0 cultures with no addition (10% fetal calf serum and erythropoietin), and with the addition of 10 HGF U/ml rIFN-R2/IL-6, 2 U/ml rIL-1, 10 U/ml rIL-3, IFN-P2/IL-6+IL-3 or IL-1+IL-3. Right half: Cells were first incubated for one week in liquid cultures with no addition and in the presence of rIFN-82/IL-6, rIL-1. rIL-3, IFN-P2/IL-6+IL-3 or IL-1+IL-3. The cells were then plated as above in methylcellulose for 15 days with PHA-induced leucocyte conditioned medium (containing all CSF) and colonies counted, The stimulation of mixed colonies from normal bone marrow progenitor cells is significant enough to warrant the use of rIFN-R2/IL-6 in bone Smarrow transplants.

0 SExample 9. Induction of Complement Factor B in fiL"oblasts S The induction of Complement Factor B in human diploid skin fibroblasts GM8399 by IFN-S2 was studied. When used alone, the immunopurified IFN-2 S' induced the secretion of Complement Factor B but the effect was again strongly potentiated by IFN-gamma. This effect is of importance since Complement Factor 8 is an essential component of the alternative pathway *mse S* of complement which kills bacteria and parasites without need for antibodies. A local increase in resistance to such infectious agents can be expected in response to the IFN-a2 IFN-gamma combination. A o biological ussay for Factor B activity showed increase in complement activity for cell lysis. The synergistic effect of IFN-gamma and IF-32 suggest that this combination may prove very attractive.

Pharmaceutical Compositions Human IFN-A2 may be used according to the invention for the treatment of b-Past cancer, leukemia, e.g. acute myelogenous leukemia, infectious diseases caused by bacteria or parasite and in bone marrow transplants. It may be used alone or in combination with other cytokines, in particular with IFN-gamma for the treatment of infectious diseases and certain types of leukemia. The active IFN-52 may be administered by any route appropriate to the condition being treated. It may be formulated with one or more pharmaceutically acceptable carriers and systematically administered either parenterally, intravenously or subcutaneously, or enterally, e.g. in the form of a tablet, capsules, etc.

The amount of active ingredient to be administered will be determined by the physician and will depend upon several factors; e.g. the severity of the condition being treated, weight, age and general condition of the patient, the route of administration chosen and the specific activity of the active IFN-P2. Daily dosages could be in the range of about micrograms to about 800 micrograms, preferably within the range of 10-100 Ss"e micrograms per day.

e S, The pharmaceutical compositions of the invention may conveniently be presented in unit dosage form and may e prepared by any of the methods o well known in the art.

S

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a S f

Claims (2)

1. A recombinant vector comprising a DNA sequence which codes for a polypeptide comprising the amino acid sequence of mature human interferon- 02 and regulatory regions which are positioned in a way that expression in bacterial cells of the polypeptide comprising the amino acid sequence of mature human interferon-02 is possible, said vector being selected from the plasmid pKK3 2 7 and the plasmid pTLS 2

501. 2. A microorganism transformed by a recombinant vector according to claim 1. 3. A microorganism according to claim 2 which is E.coli JM 105/pKKO 2 7 (ATCC 67583). 4. A microorganism according to claim 2 which is E.coli JM 101/pTL0 2 501 (ATCC 67584). A process for producing an unglycosylated polypeptide comprising the amino acid sequence of mature human interferon-02, which comprises culturing a transformed microorganism according to any of claims 2 to 4, causing the microorganism to express said polypeptide and recovering it. go Dated this 11th day of May, 1994 Yeda Research and Development Company Limited By its Patent Attorneys Davies Collison Cave 94051I ,q-Aopermns,8387-po. 131,26 9b b 27 ABSTRACT Human IFN-p2/IL-6, its purification and use. Monoclonal antibodies to IFN-p2/IL-6 and hybridomas producing them and production and purification of glycosylated and unglycosylated IFN- p2/ IL-6 00 0 are disclosed. The proteir useful in the treatment of breast cancer, 50 .0 leukemia, infectious diseases and bone marrow progenitor cell disorders. 00 0 0 00 00 0 0 0.S 0 0* 0 4 .0 0 000000 9 0 0000 OS 0. 0 000. 0 0000 0 000000 0 00 *0 0 000 0 040000 4 0 91 IOISjmsicmc.012,28813,Iv,27