AU649242C - Amino acids, peptides or derivatives thereof coupled to fats - Google Patents
Amino acids, peptides or derivatives thereof coupled to fatsInfo
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- AU649242C AU649242C AU70336/91A AU7033691A AU649242C AU 649242 C AU649242 C AU 649242C AU 70336/91 A AU70336/91 A AU 70336/91A AU 7033691 A AU7033691 A AU 7033691A AU 649242 C AU649242 C AU 649242C
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Description
Amino acids, peptides or derivatives thereof coupled to fats The present invention relates to novel compounds in which peptides, amino acids or derivatives thereof are bound to other molecules which facilitate the use of such peptides, amino acids or derivatives thereof.
In recent years there have been substantial advances made in the area of developing biological reactive peptides and peptide based compounds. One of the main difficulties encountered in the use of εuch compounds is the absence of an effective delivery system. It haε been shown that antibodies raised against small peptides are active against large proteins (e.g. foot and mouth virus), however, such small peptides are rarely antigenic in the absence of an appropriate adjuvant. The present inventor has discovered a novel means by which peptides and/or amino acid(s) and peptide derivatives may be linked to other molecules which may facilitate the therapeutic use of such peptides, amino acids or derivatives thereof. In addition, the preεent inventor haε produced novel compositions having these characteristics.
Accordingly, in a first aspect the present invention conεists in a compound of the following formula: -
CH2O - Ri
Y - NH - C - CH2O - R2
I
CH2O - R3
in which Y represents an amino acid, peptide or derivative thereof and R1 R2 and R3 are the same or different and are either hydrogen or fatty acids.
In a second aεpect the preεent invention conεiεts in a compound of the following formula: -
Y - NH - CH2 - CH20 - R4
in which Y represents an amino acid, peptide or derivative thereof and R4 represents hydrogen or a fatty acid.
As will be recognised by personε εkilled in the art the compound of the first aspect of the present invention consists of an amino acid, peptide or derivative thereof linked to a tromethamine derivative to which is optionally linked a fatty acid(s). Similarly, the compound of the second aspect can be recognised as an amino acid, peptide or derivative thereof linked to an ethanolamine derivative to which is optionally linked a fatty acid. In a preferred embodiment of the first aspect of the present invention, each of j_, and R3 are a fatty acid, and more particularly, are each the same fatty acid. It iε presently preferred that the fatty acid has a carbon chain of 3 to 18 carbon atoms, more preferably 10 to 18 carbon atoms, most preferably 16 carbon atomε. In the same manner, it is preferred that R4 iε a fatty acid having a carbon chain of 3 to 18 carbon atomε, more preferably 10 to 18 carbon atomε, and most preferably 16 carbon atomε. in a further preferred embodiment of the firεt and εecond aεpectε of the preεent invention Y iε cysteine. It iε believed that cysteine in this position would be used to create a generic reagent(s) that could be coupled to free cysteines in proteins, peptides or derivatives thereof using standard croεs linking reagents.
The novel compounds of the present invention enable alternative preεentation of an amino acid, peptide or derivative thereof due to itε position of attachment of fatty acids. Some of the potential uses of the novel
compoundε of the preεent invention are:-
1. immune enhancing affectε of fatty acid-peptide conjugateε. A large amount of reεearch haε been conducted into the potentiation of the immune reεponεe uεing fatty acid-peptide complexes. It is known that the presence of fatty acidε does lead to an enhanced immune responεe to the peptide (Baεchang,
1989, Tetrahedron, 4_5:6331-6360; Floc'h et al, 1984, Drugε Future, 9_: 763-776; Jung et al, 1985 Angewandte Chemie Int. Ed. Eng. 24 (10) pp872-873.
2. Slow releaεe delivery of peptideε. Lipoεomeε have been uεed as delivery and slow releaεe vectorε. (Moroder and Muriol, 1989, in_ Peptideε Chemiεtry, Structure and Function, J.E. Rivier & G.R. Marεhall Edε pp 811-812). Attaching fatty acids to peptides would help their incorporation into liposomes as well aε be a method of incorporating peptideε into "oily depotε" (US 4829142; Gregoriadiε, 1989, i_n Targeting of Drugε : Implicationε in Medicine, F.H.D. Roerdinl & AM Kroon Eds pp 1-29).
3. Altering the mechanism of action. (De Vrije et al
1990, J. Mol Microbiol., _4 : 143-150). Fatty acids attached to peptide hormones etc. could alter their biological activity e.g. by prolonging biological half life.
4. Fats are absorbed intact from the digestive syεtem. It iε poεεible that a peptide/fatty acid complex could be ad iniεtered orally to deliver bioactive peptideε and/or antigenic peptideε. 5. Tromethamine (TRIS) in general haε a variety of industrial uεeε including an emulεifying agent for cosmetic creams and lotions. Therapeutically uεed as an alkalizer (Merch Index, Vol 11, entry 1536). It is poεεible that some of these useε could be improved by the addition of amino acidε or peptideε optionally
with fatty acidε (Molinero et al 1988, JAOCS, 65: 975-978).
The preεent inventorε enviεage that by attaching peptideε or peptide derivativeε by their C-terminal to either TRIS- tripalmitate (or other mono, di or tri conεtructs with various fatty acids) or amino acid-TRIS-fatty acid constructε it will be possible to facilitate interaction of the peptideε-fatε with a variety of lipid structures (both natural and artificial) e.g. liposomes of various types, cell walls, membraneε etc.
(ethanolamine and similar compoundε may be uεed in place of TRIS). By thiε manner alternative methodε can be derived for the delivery and in vivo preεentation of peptideε and for modifying their mode of action and antigenicity. For example, the uεe of εynthetic antigens in variouε veterinary and human pharmacology εtill εetε the very important problem of finding a εuitable and inexpenεive carrier devoid of side effects. Carrierε uεed in animal trialε, such as keyhole limpet haemocyanin (KLH) , bovine serum albumin, ovalbumin and the tetanus toxoid are generally unsuitable for human application due to side effects such as immunogenicity. In addition some of them (e.g. KLH) have the additional problem of being very expensive. Potentially more acceptable synthetic substitutes have been investigated by a number of laboratories. The full chemical syntheεis of the N-terminal lipopeptide of the outer membrane of E.coli haε been deεcribed by G. Jung and hiε collaboratorε (Bessler et al 1984, Biochem. Biophyε. Reε. Comm. 121, pp55-61 and Jung 1988 "Low Molecular Weight Lipopeptide Carrier-Adjuvant Syεte ε for Immuniεations: Developments and Resultε" in Peptide Chemistry T. Shiba and S. Sakakibara Ed. p.751-758) and intensive εtudieε of the variouε peptide conjugates have been described. These compoundε conεiεt
of three fatty acid molecules linked to S- (dihydroxypropyl) cysteine attached to a tetrapeptide of the sequence Ser-Ser-Asn-Ala. This type of construct and analogues of it have itogenic activity and can used to anchor a variety of biological agents in lipid membranes.
A completely synthetic, low molecular weight vaccine against foot and mouth diseaεe virus (FMDV) was developed in this manner (Weismuller et al 1989, Vaccine 1_, pp29-33). A εequential epitope of FMDV εurface glycoprotein (135-154) coupled to the tripalmitoyl-S-glyceryl-cyεteinyl- εerine-εerine moiety induced long laεting high protection againεt foot and mouth diεease after a εingle adminiεtration without any adjuvant or carrier. Similarly, a vaccine againεt influenza viruε haε been prepared (Deres et al 1989. Nature 342, pp561-564). Other biological effects noted for this type of construct include the induction of cytotoxic T lymphocytes (Deres et al, as above), the release of cytokines εuch aε interferon and tumour necroεiε factor alpha from macrophages (Hauεchildt et al 1990. Eur. J. Immunol. 20, pp63-68) and the activation of neutrophilε (Seifert et al 1990. Biochem. J. 267, pp795-802).
A εecond novel oligopeptide delivery syεtem for poorly abεorbed peptideε and drugε haε been deεcribed by Toth et al 1989 ("A Novel Oligopeptide Delivery Syεtem for Poorly Absorbed Peptideε and Drugε" in Peptideε, Chemiεtry, Structure and Function, pageε 1078-1079). Thiε system iε baεed on the development of synthetic, non natural amino acids with long alkyl side-chainε; εo called "fatty amino acidε".
The covalent attachment of theεe compoundε to variouε drugs has been reported to have increased oral absorption as well aε overcoming the limited paεεage of e.g. GABA acroεs the blood-brain barrier. TRIS and ethanolamine seem to be ideal bi-functional
molecules to join peptides (via their carboxyl group to the NH2 group of TRIS or ethanolamine) and fats (via ester linkage to the hydroxyl group(s) of TRIS or ethanolamine). To demonstrate the feasibility of thiε approach εeveral amino acidε and peptideε have been reacted with TRIS and the productε characteriεed. Four of theεe, Z-Ala-TRIS, Z-Gly TRIS, Z-Leu-TRIS and BOC-Ala-Ile-Phe-TRIS were succeεεfully palmitoylated and purified. As well as constructε of 2-Ala-TRIS with sloster chain fatty acids. After removal of the
N-protecting group, the free amino terminal would be available to react with other amino acids or peptides either by enzymatic procedures or by traditional methods of peptide chemiεtry. In order that the nature of the preεent invention may be more clearly understood preferred forms thereof will now be described with reference to the following examples and Figures in which: -
Fig. 1 showε a time profile of Z-Ala-OMe pluε TRIS incubated at 60°C, pH8 in 60% DMF (—{U— ) percentage efficiency; (— -4 ) percentage εubεtrate uεed after 7 dayε;
Fig. 2 shows substrate preference; 60°C incubation for 18 hours, % efficiency ( g ) and substrate used ( βt ) vary with ester type and the nature of the amino terminal blocking group;
Fig. 3 showε temperature profile: BZ-Ala-OMe pluε
TRIS; 60% DMF pH 8.0 18 hourε ( + ) percentage substrate used;( £__ ) percentage efficiency); Fig. 4 showε pH profile Z-Ala-TRIS; 60% DMF incubated 2 days 60°C (—£D—) efficiency; (• — ) percentage subεtrate uεed;
Fig. 5 DMF profile Z-Ala-TRIS; pH 9 incubated 2 dayε 60%C (—ø— ) efficiency; ( ) percentage subεtrate uεed;
Fig. 6 εhowε 3_H-thymidine uptake in RD10 cellε
) ATP3; ( ♦— ) ZATP3; and
Fig. 7 εhowε 3f_-thymidine uptake in U937 cellε
(—£. ) ATP3; ( — ) ZATP3. MATERIALS AND METHODS
Abbreviationε: -
TLC Thin layer chromatography
HPLC High Performance Liquid Chromatography
NMR Nuclear Magnetic Reεonance GLC Gas-liquid chromatography
DCM Dichloromethane
DCC Dicyclohexylcarbodiimide
DCU Dicyclohexylurea
DMAP Dimethylaminopyridine DMF Dimethyl formamide
Z Benzyloxycarbonyl
Bz Benzoyl
BOC Tertiary, butyloxycarbonyl
TRIS Tromethamine OMe, OEt, OBzl Methyl, Ethyl and Benzyl esterε resp.
Ala, Leu, Gly, The amino acids:- alanine, leucine, lie, Phe, Tyr , glycine, iεoleucine, phenylalanine,
Arg, Trp, Lyε & tyrosine, arginine, tryptophan, lyεine
Val and valine reεpectively A. CHEMICAL AND ANALYTICAL METHODS
All εolventε were analytical reagentε and thoεe used for HPLC were especially purified for liquid chromatography.
THIN LAYER CHROMATOGRAPHY i) Plates:- a) Pre-coated TLC plates, Silica gel 60 (without fluorescence indicator, Merck 5721) 20x20 cm, 0.25 mm thicknesε. b) TLC aluminium εheetε, εilica gel coated (with fluoreεcence indicator) DC Alufolien Kieεelgel 60
20x20 cm, 0.2 mm thicknesε, Merck 5554. c) TLC aluminium εheetε, pre-coated with celluloεe (20x20 cm, 0.1 mm thickneεs, Merck 5552). ii) Detection of compoundε:- a) Ninhydrin 0.1% (Merck ARt, 6758) b) 2,4 dichlorofluorescein, 0.2% in ethanol c) iodine atmosphere SOLVENT SYSTEMS a. chloroform/methanol/water 13:5:0.8* b. chloroform/methanol/aπtmonia 17% 70:35:10* c. chloroform, εaturated* d. ether, εaturated* e. petroleum ether/ether/acetic acid 70:30:1* f. butanol/acetic acid/ water/acetic acid 5: 2:3* g. petroleum ether/ether/acetic acid 50:50:1* h. ethyl acetate εaturated with water* i. ethyl acetate/chloroform/ammonia 17% 98:6:1* j. chloroform/methanol 9:1*# k. chloroform/methanol 8:2*# 1. ether/benzene/ethunol/acetic acid 40:50:2:0.2* Rf values were determined at 20°C *TLC; # Column COLUMN CHROMATOGRAPHY
Three methods were used for separation of palmitoylated (or other fatty acid acylated) TRIS constructs: - i) LH SEPHADEX
SEPHADEX LH 20 (PHARMACIA, Fine Chemicals) was packed into glasε columnε 1x25 cm) and samples eluted with chloroform/methanol mixtures at a rate of 1 ml/min. Fractionε (5 ml) were collected and their contentε analysed by TLC. Separation iε by size, ii) SILICA GEL COLUMN
Samples were loaded onto a silica gel column (BDH 60-120, size 2.6 x 30 cm) and eluted with chloroform/
methanol mixtures (9:1 or 8:2 as required) at a rate of 2 ml/min. The effluent was monitored at 260nm (ETP KORTEC, K95 UV monitor), 5 ml fractions collected and tested by TLC. Separation iε by charge and hydrophobicity. iϋ) FLORISIL COLUMN for separation of neutral lipids (a gift from Dr. Fogarthy, CSIRO Div. of Food Preservation). Chromatography waε in glasε columnε of 12 gr acid washed FLORISIL and eluted with hexane/ethyl ether mixtures according to Carroll, K.K. and Serdarevich, B. (1967) in "Lipid Chromatography Analysis" p.205-237, Dekker, New York. The effluent waε monitored by TLC. ANALYSES i)Elemental analyεeε (CHN ratio) were carried out by the Analytical Unit of the Chemiεtry Department, Reεearch School of Chemiεtry Auεtralian National Univerεity GPO Box 4, Canberra. ii) 1 C and PROTON NMR SPECTRA were done by the CSIRO Division of Bio olecular Engineering, Parkville, Vic. 3052. The compounds of the present invention behaved aε expected with a εlow exchange of protons which are good indications of peptide bonds. iii) GLC analysis for fat content were done by Analchem Consultantε Pty. Ltd. iv) HPLC. Analytical HPLC of the N-protected amino acid-TRIS compoundε were carried out uεing Millipore Waterε HPLC equipment 6000A εerieε εolvent delivery εyste , connected to a Lambda Max Spectrophotometer Model 480, an Automated Gradient controller and Model 746 Data Module. Analytical HPLC was performed on a 100 x 8 mm NovaPak C^g reverse phase radial pack cartridge and eluted at a flow rate of 2-3 ml/min. with a triethylamine phosphate pH3/acetonitrile gradient. The eluted substanceε were detected εpectrophotometrically at 254 nm. The program uεed waε a 20-100(6)5'(2) where 20-100 iε the % acetonitrile at the beginning and end; (6) is the
gradient program (6 iε linear); 5' is time in minutes; (2) is the flow in ml/min. Preparative HPLC
Separations were performed on a Millipore Waters Prep500 HPLC. The column was PrepPAK 500-Cig (125A and 55-105 micron particle size) and the eluant waε various concentrations of aqueous ethanol at 100 ml/min. Some εeparations required the addition of acetic acid at 1 ml/L. PROTEASE TREATMENT OF Z-Ala-TRIS, Z-Gly-TRIS and Z-Leu-Arg-TRIS
Z-Gly-TRIS and Z-Ala-TRIS were incubated with papain, and Z-Leu-Arg-TRIS with trypsin, to test that the action of proteolytic enzymes could regenerate the starting compounds Z-Gly-OH, Z-Ala-OH and Z-Leu-Arg-OH respectively. i) Activation of Papain
50 ul of a εuεpenεion of papain (25 mg/ml, Sigma) was incubated with 20 mM aqueous solution of EDTA (50 ul); 1 Molar solution of mercaptoethanol (25 ul); 50 mM triethylamine (25 ul); and water (100 ul) at 37°C for 30 min. ii) Digestion Examples a) Papain. Z-Ala-TRIS in 50 mmolar phosphate buffer pH 6.5 (650 ul), 20 mM ethylene-diamiεe-tetra-acetic acid (100 ul) and water to 1 ml. Activated papain (5mg/ml) waε added to a final enzyme/substrate ratio of 1:50. The reaction waε followed by HPLC on Cj_s reverse phase columns and the hydrolysiε product identified by co-chromatography with εtandardε. Blankε were incubated aε above uεing water inεtead of enzyme εolution. b) Trypεin. Z-Leu-Arg-TRIS waε incubated at a concentration between 2 and 10 mg/ml in 0.2 M ammonium hydrogen carbonate. Freshly prepared TPCK trypsin (1 mg/ml in water) was added to the solution giving a final enzyme/substrate ratio of 1:50.
Samples were incubated at 37°C and aliquotε examined by reverεe phaεe HPLC. Additional amounts of enzyme were added as required. RESULTS i) Z-Ala-TRIS and Z-Gly-TRIS.
HPLC waε used to monitor the progreεε of enzymatic hydrolyεiε. Both Z-Ala-TRIS and Z-Gly-TRIS were hydrolyεed by papain, albeit very εlowly. The main product waε identical with Z-Ala-OH and Z-Gly-OH reεpectively by co-chro atography (i.e. εpiking). This is a check that the type of linkage between the protected amino acid and TRIS iε the same in the productε made by method A and B (see below) and that it iε most likely an amide bond. In addition we have the evidence from the NMR analysis of Z-Ala-TRIS (incubation method) that the bond is an amide linkage. Also, elemental and GLC analyεes on fatty acid derivatives indicate three fat molecules per molecule of TJRIS Indicating the presence of three OH groups on the amino acid-TRIS derivative used in the esterification. ii) Z-Leu-Arg-TRIS waε hydrolyzed slowly with trypsin. The product of hydrolysiε was shown to be identical with an authentic sample of Z-Leu-Arg-OH by co-chromatography on HPLC. The amino acid-TRIS compounds were made by incubation of the N-protected amino acid eεterε and peptide eεterε with TRIS in 60% aqueouε DMF at elevated temperature (εee incubation method). A typical reaction time profile is shown in Figure 1, effect of Z verεus BZ plus ester types are shown in Figure 2 , temperature profile in Figure 3, pH profile in Figure 4 and DMF profile in Figure 5.
As additional proof that the amino acid to TRIS linkage was a true amide bond, Z-Ala-TRIS and Z-Gly-TRIS were prepared by both the "incubation procedure" and normal organic chemistry methods for comparison by TLC.
Tri-palmitoylated forms of these two compoundε were alεo prepared and compared by TLC. In a recent work by Otvoε et al on the εolid phaεe synthesis of certain glycopeptides (Peptide Research, 1989, _2, 362) the observation was made that in coupling of amino acids to carbohydrates unprotected hydroxyl groups are mostly unaffected when the symmetrical anhydride method is uεed. Symmetrical anhydrides of Z-Ala-OH and Z-Gly-OH were prepared and reacted with large excesε of TRIS. The major products were indistinguishable by HPLC, TLC and CHN ratio from Z-Ala-TRIS and Z-Gly-TRIS prepared by the incubation method. Accordingly, we are confident that this method will only interact with the NH2*gro of TRIS and not any of the CH2OH groups. It is also to be noted that only one synthetic compound is evident during the incubation method. EXAMPLES
1. PREPARATION OF Z-AMINO ACID-TRIS AND ETHANOLAMINE COMPOUNDS METHOD A - CHEMICAL SYNTHESIS (SYMMETRICAL ANHYDRIDE METHOD) EXAMPLE 1. Z-Ala-TRIS
The title compound was produced from Z-Ala-OH via the symmetrical anhydride procedure (Schussler, H. , Zahn, H., Chem Ber. ^5, p.1076 (1962) and Bodansky, M. , and
Bodansky, A. (1984) "The Practice of Peptide Synthesiε". p.102, Springer-Verlag) .
Z-Ala-OH (8g) in DCM (50ml) and a few drops of DMF was reacted with DCC(4.2g) to form the symmetrical anhydride. The DCU so formed was filtered off and the solvent removed in vacuo. The residue was diεεolved in DMF (50 ml) and added to a solution of TRIS in DMF (10 g/80ml) and stirred at room temperature for 16 hours.
Purification of the title compound was by preparative HPLC which yielded an analytically pure white product.
Yield 2.90 g (49% theory); Rf 0.49 (solvent h) and 0.78 (solvent b), and Rt 5.19' on HPLC. Anal. calc. for
C15H22N2°6 : C55.20, H6.80, N8.58. Found C55.28,
H6.87, N8.13. The compound had identical characteristics 5 to that formed by the "Incubation Method" - Method B (see
Example 3) .
EXAMPLE 2. Z-Gly-TRIS
Z-Gly-TRIS was prepared in the same manner aε Example
1 i.e. from Z-Gly-OH (7q) and purified by preparative 0 HPLC. Yield 1.50 g (29% theory). The resulting
Z-Gly-TRIS waε chromatographically identical with the corresponding compound made by method B (see Example 4).
Rf 0.36 (solvent h) and 0.66 (εolvent b) , and Rt 4.97' on
HPLC. Anal. calc. for C14H2oN2θ6 : C53.84, H6.45, 5 N8.97. Found C54.53, H6.84, N8.85.
METHOD B. INCUBATION METHOD
EXAMPLE 3. Z-Ala-TRIS
5.04 g of Z-Ala-OMe (50 mmole) waε added to 20.37 g. of TRIS (400 mmole) in DMF (252 ml) and water 168 ml) and 0 incubated at 55°C at pH 9.0. The reaction was monitored by HPLC which showed that after four days the conversion to the title compound waε complete with a 65% efficiency as determined by HPLC.
Purification by preparative HPLC gave an analytically 5 pure Z-Ala-TRIS as a white powder. Rf 0.49 (solvent h) and 0.79 (solvent b). HPLC Rt was 5.19 minutes. Anal. calc. for C15H22 2O6 : C55.20, H6.80, N8.58.
Found C56.02, H7.03, N8.00. The structure was confirmed by NMR analysiε. ° EXAMPLE 4. Z-Gly-TRIS
10.02 g of Z-Gly-OBzl waε added to 32.4 g of TRIS in
DMF (401 ml) and water (267 ml) and incubated at 60°C at pH 9.0 for two dayε. Purification by preparative HPLC gave analytically pure Z-Gly-TRIS aε a white powder. 5 Yield 3.98 g (38.3% theory); Rf 0.36 (solvent h) and 0.66
(solvent b); HPLC Rt was 4.97'. Anal. calc. for C14H20N2°6 : C53.84, H6.48, N8.97. Found C54.15, H6.56, N8.68. The structure waε confirmed by NMR analysis. EXAMPLE 5. Z-Leu-TRIS Z-Leu-OMe (5g) and TRIS (16.2 g) in DMF (200 ml) and water (134 ml) was incubated at pH 8.0 and 55°C in the manner previously described (Example 3).
Purification by preparative HPLC gave 3.60 g (54% of theory) of analytically pure title product; Rf 0.62 (solvent h) and 0.25 (εolvent i); HPLC Rt 6.37'. EXAMPLE 6. Z-Leu-Arg-TRIS
Z-Leu-Arg-Ome (5.17 g) and TRIS (10.67 g) in a εolution of DMF (132 ml) and water (88ml) was incubated at 55°C for seven days in the manner previously described. The efficiency of the reaction was 55% by HPLC. Pure product was iεolated by preparative HPLC. TLC Rf 0.70 (εolvent f) and 0.02 (εolvent b); Rt 5.37'. EXAMPLE 7. BOC-Ala-Ile-Phe-TRIS
8.6 g of BOC-Ala-Ile-Phe-OMe was added to 20 g of TRIS in DMF (240 ml) and water (160ml). The mixture was incubated at 55°C for four days (yield by HPLC 66%) and the title compound purified by preparative HPLC to give the analytically pure product (yield 1.4 g; 14% theory); Rf 0.50 (solvent h) and 0.85 (εolvent b); HPLC Rt 6.55'. Anal. calc. for 26H45N4°8 : C57.65, H8.37, N10.34. Found C58.01, H8.12, N9.57. EXAMPLE 8 Z•Ala-ETHANOLAMINE
Z-Ala-OEt (5.73 g) waε incubated in DMF (273 ml) and water (91ml) at 50°C with 91 ml ethanolamine at pH 11.
The reaction waε monitored by HPLC and after twenty hourε the formation of the title compound was complete with 82% efficiency by HPLC. Preparative HPLC yielded a chromatographically pure product (2.26 g: 38% theory); Rf 0.50 (solvent h) and 0.82 (solvent b); HPLC Rt 5.10'.
EXAMPLE 9
FORMATION OF AMINO ACID TRIS COMPLEXES
Comparative reactionε were run uεing Z-Leu-OMe; Z-Ala-OMe; Bz-Tyr-OEt; Z-Ala-OBzl; Z-Arg-OMe; Z-Val-OMe and Bz-Arg-OMe. The efficiency of the reaction of these compounds with TRIS and the rate at which the reaction proceeded are set out in Tableε 1 and 2, reεpectively.
TABLE 1
TABLE 2
EXAMPLE 10
FORMATION OF Z-LEU-ARG-TRP-TRIS
5.28 grams of Z-Leu-Arg-Trp-OMO was added to 7.6 grams of TRIS in 160 mlε of εolvent conεisting of 60% DMF and 40% H2O. The reaction was allowed to proceed at 60° for four dayε at pH 10. Z-Leu-Arg-Trp-TRIS waε recovered by preparative HPLC. Efficiency by HPLC - 43%. Rf 0.72 (εolvent f) . EXAMPLE 11 FORMATION OF Bz-Tyr-Arg-Lys-TRIS
5.025 grams of Bz-Tyr-Arg-Lys-OE was added to 7.275 grams of TRIS in 150 mis. of solvent comprising 60% DMF and 40% H2O. The reaction waε allowed to proceed at 60° for four days 50% yield by HPLC and Bz-Tyr-Arg-Lys-TRIS was recovered by preparative HPLC. 2. PREPARATION OF FATTY ACID COMPLEXES EXAMPLE 12 Z-Ala-TRIS-TRIPALMITATE
A solution of palmitic acid (8.10 g) in chloroform (100 ml) was added to a stirred solution of Z-Ala-TRIS (2.75 g) in chloroform (60 ml) and DMF (15 ml) and the mixture cooled in ice. After dropwise addition of 6.50 g of DCC in chloroform (30 ml) followed by 0.4 g of DMAP in chloroform (10 ml), stirring was continued at 0°C for two hours and then at room temperature for 18 hours. The esterification reaction waε monitored by TLC (εolvent εyεtems b, h, i and j). The resulting DCU was filtered off and the chloroform solution serially washed with water; 10% aqueous citric acid; water; 10% aqueous sodium hydrogen carbonate and water. The solution was dried over sodium sulphate and the solvent evaporated under reduced pressure. The crude product was purified by chromatography by three different methods:- LH Sephadex; silica gel and Florisil. All techniqueε gave ~nalytically pure productε that gave a εingle spot on TLC; Rf 0.72
- 17
(solvent g) and Rf 0.29 (solvent e). Yields were 62.0%, 63.8% and 68.5% respectively. Anal. chem. for C63H112N2°9 : C72.65, H10.84, N2.69. Found C72.58, Hll.22, N2.31. Fat (palmitic acid) content by GLC analysis was 70.1% indicating full esterification of all three hydroxyl groups of the Z-Ala-TRIS (Theory 68.7%). NMR results were conεiεtent with the propoεed εtructure. Productε made with Z-Ala-TRls prepared by both the chemical and incubation methodε yielded analytically indiεtinguiεhable productε. EXAMPLE 13 Z-Gly-TRIS-TRIPALMITATE
0.94 g of Z-Gly-TRIS was reacted with 3.30 g of palmitic acid; 2.5 g of DCC and 0.12 g of DMAP (as per Example 12) to give a crude sample of the title product, 2.05 g, 67% Theory. Purification on LH Sephadex and silica gel gave analytically pure product in 43% and 45% yield respectively; TLC Rf 0.18 (solvent e) and 0.85 (solvent 1).
Fat (palmitic acid) content by GLC analysiε was 70% indicating full esterification of all three hydroxyl groups of the Z-Gly-TRIS (Theory 69.6%). NMR resultε were consistent with the proposed structure. Products made with Z-Gly-TRIS prepared by both the chemical and incubation methods yielded analytically indistinguishable products.
EXAMPLE 14
Z-Leu-TRIS-TRIPALMITATE 1.10 g of Z-Leu-TRIS waε reacted with 3.30 g of palmitic acid; 2.5 g of DCC and 0.12 g of DMAP (aε per Example 12) to give a crude sample of the title product. Purification on a silica column yielded 1.78 g (54.9% Theory) of analytically pure product; TLC Rf 0.90 (solvent g) and 0.87 (solvent k). Anal. calc. for
C67H121N2°9 : C73.08,m Hll.06, N2.58. Found C73.74, H11.52, N2.29. Fat content 66.0% (Theory 66.1%). EXAMPLE 15
Z-Ala-TRIS-MYRISTATE Z-Ala-TRIS (326 mg) waε reacted with myristic acid (914 mg) in chloroform in a εimilar manner to Example 10. DCC (825 mg) and DMAP (40 mg) in cold DCM (10ml) waε added and εtirred for two hours at 0°C followed by 16 hourε at room temperature. Initial purification yielded 720 mg crude product. Precipitation from DCM/acetonitrile yielded 640 mg. (66.9% of the theoretical) of the title product. TLC gave a single spot of Rf 0.63 (solvent g) and 0.29 (solvent e) . EXAMPLE 16 Z-Ala-TRIS-TRILAURATE
326 mg of Z-Ala-TRIS and 801 mg lauric acid was reacted with 825 mg DCC and 45 mg DMAP in a manner similar to that described for Example 12. Evaporation of the solvent yielded 710 mg of crude title product. This waε further purified on a Floriεil column uεing the hexane-ethyl ether solvent mixtures aε eluants. 420 mg (49.9% of theoretical) of the title compound was recovered; TLC Rf 0.59 (solvent g) and 0.27 (εolvent e). EXAMPLE 17 Z-Ala-TRIS-TRICAPRATE
326 mg of Z-Ala-TRIS and 464.7 mg of capric acid (n-decanoic acid) waε reacted with 825 mg of DCC and 45 mg of DMAP in DCM in a manner deεcribed in Example 13. Purification on Floriεil with hexane/ether and methanol gave chromatographically pure title product; yield 480 mg (61% Theory); Rf 0.54 (εolvent g) and 0.25 (εolvent e). Anal. calc. for C45H76N2O9 : C68.49, H9.71, N3.55. Found C68.96, H10.230, N3.18. Fat content by GLC was 63% (Theory 58.9%).
EXAMPLE 18
Z-Ala-TRIS-MONOPALMITATE
700 mg of Z-Ala-TRIS waε reacted with 500 mg palmitic acid and 410 mg DCC and 20 mg DMAP in the manner εimilar to that deεcribed in Example 12 except a 1.3 molar exceεs of reagents was used inεtead of the usual 4 molar excesε to generate a conεtruct with predominantly one fatty acid attached: some di and tri-palmitoylated product would also be expected. Yield of crude, mixed products 1.12 g (Theory 1.20 g) .
Fractionation on Floriεil gave three major productε in the ratio of 0.27:1:1 with Rf'ε on εolvent g of 0.73; 0.26 and 0.0 reεpectively. Fractionε 1 and 2 are iεomeric dipalmitate derivativeε analyεing by GLC as containing two fat molecules relative to Z-Ala-TRIS (56% and 59% reεp. ; Theory 59.6%) while Fraction 3 waε conεiεtent with being a monopalmitate derivative (fat content waε 40%; Theory 42.5%). The -yield by weight of Fraction 3 waε 208 mg (24.5% Theory) . EXAMPLE 19
BOC-Ala-Ile-Phe-TRIS-TRIPALMITATE
BOC-Ala-Ile-Phe-TRIS-tripalmitate waε prepared in a εimilar manner to Example 12 from a εolution of BOC-Ala-Ile-Phe-TRIS (500 mg in 30 ml chloroform; 5 ml DMF); palmitic acid (1.1 g in 20 ml chloroform), 825 mg of DCC in 10 ml chloroform and DMAP (40 mg in 10 ml chloroform) at 0°C for 2 hourε and at room temperature for 16 hours.
After the removal of DCU, the chloroform solution was washed as previously deεcribed (water, citric acid, water, εodium hydrogen carbonate, water). After the removal of εolvent, the oily reεidue waε dried (Crude yield 1.66 g) .
The crude product waε dissolved in hexane and a portion of the solution purified on a Florisil column. Hexane: ether solvent mixtures removed the unreacted
palmitic acid and other impurities. The title product was then eluted with chloroform:methanol 50:50 (150 ml). The product waε obtained as an oil, which was lyophilised from t-butylalcohol. The white fluffy compound (770 mg; 66.4% Theory) gave a single spot on TLC; Rf 0.44 (solvent g) and 0.13 (solvent e). Anal. chem. for C74H135N4°11 : C70.71, H10.83, N4.46. Found C70.72, H10.78, N4.14. Fat content by GLC was 58% (Theory 56.9%). EXAMPLE 20
Z-Ala-ETHANOLAMINE- ONOPALMITATE
540 mg of the Z-Ala-ethanolamine was reacted with 670 mg palmitic acid, 540 mg DCC and 32 mg of DMAP in DCM and DMF as per Example 12. The mixture was evaporated to drynesε and purified on Florisil. 219 mg of product (20% theory) was isolated. TLC Rf 0.16 (solvent g), 0.14 (solvent c, twice) and 0.12 (solvent d). EXAMPLE 21 MITOGENIC EFFECTS OF Z-Ala-TRIS-TRIPALMITATE AND Ala-TRIS- TRIPALMITATE
Lipopeptides of the type Pam3-Cyε-Ser- (Lys)4 and Pan_3-Cyε-Ala-Gly are potent lymphocyte and macrophage activatorε (e.g. Reitermann et al., Biol. Chem. Hoppe-Seyler, 370, pp343-352). Theεe compounds have also been shown to induce tumour cytotoxicity (Hoffmann et al 1989. Biol. Chem. Hoppe-Seyler, 370, pp575-582) in murine bone marrow derived macrophages.
The mitogenic effects of two of our constructs, Z-Ala-TRIS-Tripalmitate and Ala-TRIS-Tripalmitate were examined as followε:
INCORPORATION OF 3H-THYMIDINE
3H-thymidine incorporation experimentε were carried out in Linbro-Titertek microtitre plateε (Flow Laboratorieε, inc., U.S.A.) following the method of L. Bradley in "Selected Methods of Cellular Immunology (1980)
Edε. B.B. Miεhell and S.M. Shiigi; Publiεher. W.H. Freeman and Co., U.S.A. ppl56. The teεts were carried out externally by Dr. D. Rathjen of Peptide Technology Ltd., Dee Why, N.S.W. Cell lines tested were:-
1. RDlO; a B-cell lymphoma (obtained from the Centre for Immunology, Sydney University, Sydney, NSW.
2. U937; a human monocyte-like3 histiocytic lymphoma (source: American Tisεue Culture
Collection) . METHOD (EXAMPLE)
RDlO cellε (at a cell denεity 5xl05/ml, 100 ul/well) were cultured for 20 hourε at 37°C with 5% CO2 in RPMI 1640 medium, εupplemented with 10% Fetal Calf. Serum. Sampleε to be teεted were mixed and εonicated with thiε medium at a concentration of 5000, 500.50 and 5-ug/ml and 100 ul aliquotε added to the cellε. The cellε were pulεed with 0.5 uCi/well of 3H-thymidine and the plates incubated for four more hours before harveεting (Titertek Skatron harveεter, Lier, Norway) . Incorporated radioactivity waε meaεured by liquid scintillation counting. All asεays were performed in triplicate. RESULTS
Figures 6 and 7 summarize the results.
3H-thymidine uptake by RDlO cells showed a 3-4 fold increase in cell proliferation compared to control. Thiε iε in the range of other known mitogenic compounds e.g., phytohaemagglutinin gives a 5-10 fold increase under these conditions. The sampleε containing Ala-TRIS-Tripalmitate, where the N-terminal of the Alanine iε free, εhowed a εlightly diminiεhed response compared with Z-Ala-TRIS-Tripalmitate. Thymidine incorporation of the U937 cells was, in
slight contrast, close to control, and at higher concentrations indicated slight cytotoxic effects. As in the experiments uεing B-cellε, Ala-TRIS-Tripalmitate εampleε εhowed εlightly diminiεhed reεponεeε compared with Z-Ala-TRIS-Tripalmitate.
It will be appreciated by persons skilled in the art that numerous variations and/or modifications may be made to the invention aε shown in the εpecific embodiments without departing from the spirit or scope of the invention as broadly deεcribed. The present embodiments are, therefore, to be considered in all respects aε illustrative and not restrictive.
Claims (12)
1. A compound of the following formula: -
CH2O - Rx
Y - NH - C I - CH 0 - R2
CH20 - R3
in which Y represents an amino acid, peptide or derivative thereof and Rx R2 and R3 are the same or different and are either hydrogen or fatty acidε.
2. A compound aε claimed in claim 1 in which each of Rx, R2 and R3 are fatty acidε.
3. A compound aε claimed in claim 2 in which R , R2 and R3 are the εame fatty acid.
4. A compound as claimed in any one of claims 1 to 3 in which the fatty acid has a carbon chain of 3 to 18 carbon atoms.
5. A compound as claimed in claim 4 in which the fatty acid has a carbon chain of 10 to 18 carbon atomε.
6. A compound aε claimed in claim 5 in which the fatty acid haε 16 carbon atomε.
7. A compound as claimed in any one of claims 1 to 7 in which Y is cysteine.
8. A compound of the following formula: -
Y - NH - CH - CH2O - R4
in which Y represents an amino acid, peptide or derivative thereof and R4 represents hydrogen or a fatty acid.
9. A compound as claimed in claim 8 in which R4 is a fatty acid having a carbon chain of 3 to 18 carbon atoms.
10. A compound aε claimed in claim 9 in which the fatty acid has a carbon chain of 10 to 18 carbon atoms.
11. A compound as claimed in claim 10 in which the fatty acid has 16 carbon atoms.
12. A compound aε claimed in any one of claims 8 to 11 in which Y is cysteine.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AU70336/91A AU649242C (en) | 1989-12-22 | 1990-12-19 | Amino acids, peptides or derivatives thereof coupled to fats |
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AUPJ8035 | 1989-12-22 | ||
AUPJ803589 | 1989-12-22 | ||
AU70336/91A AU649242C (en) | 1989-12-22 | 1990-12-19 | Amino acids, peptides or derivatives thereof coupled to fats |
PCT/AU1990/000599 WO1991009837A1 (en) | 1989-12-22 | 1990-12-19 | Amino acids, peptides or derivatives thereof coupled to fats |
Publications (3)
Publication Number | Publication Date |
---|---|
AU7033691A AU7033691A (en) | 1991-07-24 |
AU649242B2 AU649242B2 (en) | 1994-05-19 |
AU649242C true AU649242C (en) | 1995-05-25 |
Family
ID=
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