AU611716B2 - Multi-layered gaseous phase cultivation - Google Patents

Description

PCT

AIZ0-63O61/8 fifi;If*1AM~S~ bP K ft (Si) ~3~ilft AOIK 63/04, C12N 1/20, 5/00 (11) MIM4,1-a WO 89/ 02218 Al (43) NM:J PAE 19893,q 236B(23 03 89) (21) R[*l:B9:9-j3 PCT/JP88/00925 (22) IAS~ffi 9 B 1 988 I2 6(12. 09. 88) (31 441A06 6 2 2 3 17 2 WOM662-142316 U (32) ffl B 1987!94 616sB(16. 09. 87) 1987-9R18B18. 09. 87) (33) fIIXt3 -Y JP (71) WlXi\k (t~-RhSvR c) VPr! 1--f 1 (YUUQEN KAI SYA PARASIGHT)XJP/JP) 7 9 0 AYEZYAK8ZAMT3EB 39T 3 3 4 Kagoshima, (JP) (72) -A 6.U Z4 VIr (NOJ I\A, Yo u k o i J P/J P N(NOJ I A, Hi s a t ake) JP/JP) XD.J. P. 25 mAY 1989 T 8 9 0 MYERAP iaMA" 4 T 14-10- 24 Kagoshima, (JP?) 81 t97_9 )AUSTRALIAN AT(Eafli4F), AU:, BE(Riiil, BR, CM- ~17 APR 1989 DE(DJli4K), OK, F1, FR. WiONae t, G B iW ),H040 H L, I T(mJai;, K R, LL'. AJiMtV"r,, NL PATENT OFFICE NO, RO, SE(w)4t SU, US.

(54) Title: MULTI-LAYERED GASEOUS PHASE CULTIVATION 4, 3 /1 00 6 6 0,op 0 0, 0 ov 0 0 o 0 0 0 0 0 0 000 oc 0 (57) Abstract This invention relates to a method of providing three-dimensional mass-cultivation of aquatic organims kept immediately below multi-layered gaseous phases, in which the problem of oxygen supply is solved by fixing a large number of containers in the water and allowing air to stay in multiple layers. When air is supplied from below, it can pass from lower containers to upper containers through hollow tubes and a natural underwater environment can be produced at all points in the water.

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DESCRIPTION

MULTI-LAYERED GASEOUS PHASE FORMATION METHOD AND ITS APPARATUS TECHNICAL FIELD: This invention relates to a multi-layered gaseous phase formation method and its appratus which makes it possible to conduct mass cultivation of all aquatic organisms.

00 BACKGROUND ART: Conventional cultivation for mass cultivation of fish relies on the method which causes forceful aeration by use of water wheels or aerators to dissolve oxygen into ponds. Even if an attempt is made to cultivate a greater mass of fish in deeper ponds, productivity of cultivation cannot be improved due to oxygen deficiency. Therefore, the increase 0* *in the scale of cultivation results inevitably in the the increase in pond area. Furthermore, it is •difficult to remove feed debris and excretes through- Se out the pond or the like having a predetermined capacity and eventually, this results in the problem of contamination of the pond itself. In addition, since large rotary vanes are disposed inside a tank so as to supply oxygen into a culture solution in the conventional mass cultivation of cells and 0379k/SC Smicroorganisms, there occurs the problem of physical destruction of the cells and microorganisms.

DISCLOSURE OF INVENTION: The present invention provides a multi-layered gaseous phase formation method comprising the steps of: disposing a plurality of open mouthed containers in a liquid filled tank with their open mouths downwards and in at least two layers; providing each container with a gas vent to communicate a point in the interior of the container below the uppermost interior point with the exterior of the container; arranging the containers such that each container of one layer is above the exterior end of a gas vent of a container of the next lowermost layer; and feeding gas to the containers of the lowermost layer.

S e The invention also provides an apparatus for multi-layered gaseous phase formation comprising: a liquid tank; a plurality of containers disposed in the tank and arranged in at least two layers, the containe-rs having open lower ends and closed upper ends; means to supply gas to the lowermost layer of containers.

each container including a gas vent communicating the container interior with its exterior and arranged such that gas escaping from a container of one layer is directed into a S-2- -3 container of the next uppermost layer, the vent positioned below the upper end of the container and thereby causing a gas pocket to form in the container.

The container may be formed as a container 2 having a substantially H-shaped section, and sand, small stones, and the like are put into open space on -the upper surface so as to form the cultivation places of the acuatic organisms.

in this manner, it becomes possible to permit a gas such as air to stay horizontally in mulitple layers in the cultivation water in the liquid tank, to establish an oxygean-rich state under the gas staying state in the same way as below the ordinary water surface and to cultivate various aquatic organisms.

BRIEF DESCRIPTION OF DRAWINGS: The present invention will be explained in further detail with reference to the accompanying drawings.

so 4 49 ISc 0379k/SC Fig. 1 is a sectional view showing an embodiment of the present invention and Fig. 2 is an explanatory view showing another embodiment of the present invention but with details omitted for clarity.

BEST MODE FOR CARRYING OUT THE INVENTION: In Fig. 1, reference numeral 1 represents a liquid tank and reference numeral 2 a shallow vat. A plurality of (four, in Fig. 1) vats 2 are disposed with their open surface 2a facing downward as shown in Fig. 1. These vats 2 are disposed at four oil levels inside the tank 1 with predetermined gaps between them by means of a support pole 3 which is located substantially at the centre of the tank 1. Reference numeral 4 represents an air feed pipe which is connected to a compressor (not shown in the drawings). The outlet 4a of this air feed pipe 4 is extended in such a manner as to feed the air to the lower open surface of the vats 2 of the lowermost stage. Reference numeral 5 represents a round vent hole which is bored at part of the upper wall of each vat 2. The vent holes 5 are shown disposed at different positions to one another in the embodiment shown in Fig. 1. Reference numeral 6 represents a cylinder (partition wall) implanted at each vent hole 5 for guiding the air into each vent hole 5. The height of this cylinder 6 is a little lower than the height of the peripheral wall of the vat 2. Incidentally, though the cylinder 6 represents the partition wall in this embodiment, the present invention is not particularly limited to the cylinder 6. For j ample, the 4-.

0379k/sc partition wall may be square and in short, it may be a partition-like member having -the function of guiding the gas via the vent hole 5 to the vat 2 above it.

Next, the function of the present invention having the construction described above will be explained.

First of all, when the air is pressure-fed by the air feed pipe 4 via the lower open surface 2a of the vats 2 of the lowermost stage, the gas accumulates in the vats 2 until the water level is at the base of the cylinders 6 of the vats 2 of that stage. When the supply of the air is continued, the air rises up through the vent hole 5 of the cylinder 6 and is again :00permitted to accumulate in the vats of the next higher stage.

S When this air feed operation is repeated, the air is caused to oeo accumulate in each space 7 of each vat 2 which is disposed in the four stages. Thus, in accordance with the present invention, the unstable gas existing in water can be allowed to accumulate to the depth of cylinder 6 and can be controlled accurately. Accordingly, the spaces in the vats 2 become the air zones and at the same time, become suitable cultivation places of aquatic organisms. Accordingly, a large number of air layers and a large number of cultivation places can be disposed inside one liquid tank 1.

*0*00*Fig. 2 shows another embodiment of the present invention. In this embodiment, for clarity, the air feed pipe 4, support pole 3 and vent holes 5 are not shown. In this embodiment, H-shaped vats 12 are formed as shown in the drawing, and sand and small etones 8 are put on the open spaces on their upper surface so as to form cultivation areas for aquatic organisms and to accomplish the atmospheric underwater environment which is the P PA Z-7P_ r if j 6

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0379k/SC same as the environment below -the natural water surface. The construction of the vats are the same as those of the foregoing embodiment of Fig. 1. incidentally, the shape of the vat is not particularly limited to the shape described above, but the upper part of the vat may be kept free as the space capable of storing sand or the like.

In the embodiments described above, the shape of the vat 2 and 12 are not particularly limitative. In short, the vat may be disposed with its open surface facing downward. The gap between the vats 2 is determined appropriately in accordance with the size and kind of the aquatic organisms. Furthermore, the number of the vent holes 5 to be formed in one vat 2 is not particularly limited to one but may be more than one.

INDUSTRIAL APPLICABILITY: As described above, in accordance with the present invention, which is suitable for cultivating various aquatic organisms it is possible to keep oxygen, nitrogen and any other desired gases suitable for the cultivation of all aquatic organisms at desired positions in water and to control easily the mass of such a gas. Accordingly, the present invention is suitable for the Mass cultivation of aerobic and anaerobic organisms and further chemosynthetic bacteria. Furthermore, the present invention is suitable for the cultivation of fish and shell fish, animal and plant cells and microorganisms as the nuclei of biotechnology.

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Claims (9)

1. An apparatus for multi-layered gaseous phase formation comprising: a liquid tank; a plurality of containers disposed in the tank and arranged in at least two layers, the containers having open lower ends and closed upper ends; means to supply gas to the lowermost layer of containers. each container including a gas vent communicating the container interior with its exterior and arranged such that gas escaping from a container of one layer is directed into a container of the next uppermost layer, the vent positioned *9@ below the upper end of the container and thereby causing a gas pocket to form in the container.

2. The apparatus of claim 1 wherein the gas vent comprises a tube passing through the wall to the container.

3. The apparatus of claim 1 wherein the gas vent is one or more orifice in the container wall.

4. The apparatus of any one of claims 1 to 3 wherein, in cross section the containers have an H-section, and the upper surface supports earth and plants.

5. A multi layered gaseous phase formation method, comprising the steps of: disposing a plurality of open mouthed containers in a liquid filled tank with their open mouths downwards and in at least two layers; providing each container with a gas vent to communicate a point in the interior of the container below the uppermost interior point with the exterior of the container; i379k/SC arranging- the containers such that each container of on(eA layer is above the exterior end of a gas vent of a containeor of the next lowermost layer; and feeding gas to -che containers of the lowermost layer.

6. The method of claim 5 further .including the stop of- providing a surface for the support of aquatic plants.

7. The method of claim 5 or claim 6 wherein the step of providing a gas vent comprises providing a tube passing through the wall of the container.

8. The method of claim 5 or claim 6 wherein the stel? of providing a gas vent comprises providing an orifice in the container wall.

9. An apparatus for multi-layered gaseous phase formation, substantially as herein described with reference to the Sdrawings. A method of multi-layered gaseous phase formation, substantially as herein described with reference to the drawings. DATED this 30th day of August, 1990. 0 YUUGEN KAISYA PARASIGHT By Its Patent Attorneys ARTHUJR S. CAVE CO. A)I 0379k/SC