AU609794B2 - A test set and a process for the determination of antibiotics in milk and a novel streptococcus thermophilus strain to be used therein - Google Patents

A test set and a process for the determination of antibiotics in milk and a novel streptococcus thermophilus strain to be used therein Download PDF

Info

Publication number
AU609794B2
AU609794B2 AU14158/88A AU1415888A AU609794B2 AU 609794 B2 AU609794 B2 AU 609794B2 AU 14158/88 A AU14158/88 A AU 14158/88A AU 1415888 A AU1415888 A AU 1415888A AU 609794 B2 AU609794 B2 AU 609794B2
Authority
AU
Australia
Prior art keywords
test set
milk
antibiotics
indicator
streptococcus thermophilus
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
AU14158/88A
Other versions
AU1415888A (en
Inventor
Annika Mayra-Makinen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Valio Oy
Original Assignee
Valio Meijerien Keskusosuusliiki
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Valio Meijerien Keskusosuusliiki filed Critical Valio Meijerien Keskusosuusliiki
Publication of AU1415888A publication Critical patent/AU1415888A/en
Application granted granted Critical
Publication of AU609794B2 publication Critical patent/AU609794B2/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/14—Streptococcus; Staphylococcus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/18—Testing for antimicrobial activity of a material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/315—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Streptococcus (G), e.g. Enterococci

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Dairy Products (AREA)
  • Saccharide Compounds (AREA)
  • Compounds Of Unknown Constitution (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The invention relates to a test set suitable for the determination of antibiotics in milk and comprising a Streptococcus thermophilus T101 concentrate and a water-based protective agent in a dilution ratio of 4 to 5 x 10<-><2>. The invention is also concerned with a novel Streptococcus thermophilus T101 strain to be used in the test set and with a process of determining antibiotics in milk by means of said test set.

Description

COMMONWEALTH OF AUSTRALIA 6 K..
PATENTS ACT 1952-69 COMPLETE SPECIFICATION
(ORIGINAL)
Class Application Number: Lodged: Int. Class Cprnpleta Specif icatlon Lodged: Accepted: .0t# Published: Priority: Related Art, a C NRape of Applicant: Address of Applicant: VALIO MEIJERIEN KESKUSOSUUSLIIKE Actual Inventor: Address for Service: Kalevankatu 56, SF-00180 Helsinki, Finland ANNIKAL MAYRA-MAKINEN EDWD. WATERS SONS, 50 QUEEN STREET, MELBOURNE, AUSTRALIA, 3000.
Complete Specification for the invention entitled: A TEST SET AND A PROCESS FOR THE DETERMINATION OF ANTIBIOTICS IN MILK AND A NOVEL STREPTOCOCCUS THERMOPHILUS STRAIN TO BE USED THEREIN The following statement is a full description of this invention, including the best methtod of performing it known to :-u Seal of Company and Signatures of its Olficers as by prescribed by its Articles of Association, A s s o c l t, D. B. Mischlewski A test set and a process for the determination of antibiotics in milk and a novel Streptococcus thermophilus strain to be used therein The invention relates to a test set suitable Sfor the determination of antibiotics in milk. The invention is also concerned with a novel Streptococcus thermophilus strain to be used in the test set and a Scc process for the determination of antibiotics in milk ao 6 by means of said test set.
0ce In many situations it is of vital importance to be able to detect the presence of small amounts of antibiotics. This is the case in food industries, for instance, where the increased use of antibiotics and chemotherapeutic substances in the treatment of animals has created a need for a simple, reliable and sensitive process of determination. Since antibiotics ;are used also in the treatment of dairy cows and since antibiotic residues in milk may both cause health hazards and be disadvantageous for food technological reasons, it is especially important to develop processes suitable for an accurate and rapid screening of o milk.
Antibiotic residues in milk are generally detected by microbiological processes which utilize the fact that bacteria are able to produce acid, reduce colours and produce growth on an agar medium. These processes are based on the bactericidal, inhibitory and morphological effect of antibiotics on certain microorganisms.
I The Thermocult disk technique is an agar diffusion technique which is widely used in Finland and S accepted as an official antibiotic determination procedure. In this technique the test organism is B. stearothermophilus var. calidolactis. It has been
V
2 developed on the basis of an IDF standard process (IDF 1970. Detection of Penicillin in Milk by a Disk Assay Technique. International Standard FIL-IDF 57. Brussels).
A process of corresponding sensitivity is disclosed by van OS et al., Diffusion Test for the Determination of Antibiotic Residues in Milk. Neth. Milk and Dairy J. 29 (1975) 16. The Delvotest process, too, uses B. stearothermophilus var. calidolactis as the i ctest organism. A sample (0.1 ml) is pipetted on agar S, contained in an ampoule, and a tablet containing nutrients and a pH indicator is added to the ampoule.
The process is based on the acid producing capability Ecc 'of the test organism. The ampoules are incubated at S' 64 0 C for 2.5 hours. The evaluation is based on the colour change of the agar layer.
Standard techniques further include the Intertest (BCP-Test). The test microbe used in the process S' is Str. thermophilus. A test tablet containing a lyoc*c philized culture of the test microbe, nutrients, and a C t t V pH indicator (bromocresol purple) is added to a milk sample. The incubation time is 4 hours at 45 0 C. If the sample does not contain any antibiotic, the colour of the solution turns from blue to green and further to yellow. The amount of the antibiotic can be determined to some extent on the basis of the colour by comparing to a colour map (THOROGOOD et al., An Evaluation on the Charm Test A Rapid Method for the Detection of Penicillin in milk. J. Dairy Research 50 (1983) 185).
A drawback of these processes is their insufficient sensitivity in view of the needs of milk technology.
The determination of antibiotic residues in milk by means of chemical or physico-chemical processes is considerably less usual than the use of micro- 3 biological processes. Colorimetric and chrLnatographic processes require skilled labour and often a complicated and expensive analyzing equipment. The processes are seldom suitable for routine analyses.
The Charm test (CHARM, A 15-minute Assay for Penicillin and other Antibiotics. Cultured Dairy Products J. 14 (1979) 24) is based on the detection of radioactivity. A lyophilized culture of B. stearothermophilus culture and lyophilized 14 C-labelled penicillin are added to a sample. The amount of 14
C
CO
contained in the bacterium cells is detected by a "0a Geiger counter; the lower the penicillin concentration cnco of the sample, the higher is the reading of the Geiger counter. The detection time is only 15 minutes and the sensitivity of the process is 0.005 I.U. of penicillin per ml. This process, either, is not suitable for routine use; it is expensive and complicated and requires 00 skilled persons and expensive equipment to be carried out.
a Thus there is still a practical need for a sensitive process which is as broad-spectrum as possible. The process should also be simple and it should be possible to carry out the process by an equipment ready for use, whereby the test does not require skilled persons, but can be carried out e.g. on a farm.
These advantages are obtained by means of a test set according to the invention, which is characterized in that it comprises a Streptococcus thermophilus TI01 concentrate and a water-based protective agent in a dilution ratio of 4 to 5 x 10-2. The determination is carried out according to the invention by adding a sample to a test set comprising a Streptococcus thermophilus T01 concentrate and a water-based protective agent in a dilution ratio of 4 to 5 x 10 2 r
S
4 and possibly an indicator, and if the test set does not comprise an indicator, an indicator is added, too; incubating the test set and the sample at 38 to 42 0
C
for about 4 hours; and evaluating the colour.
The invention is based on the novel Streptococcus thermophilus T101 strain, which has been isolated at the Lammi creamery of Valio. The strain has been deposited at the Deutsche Sammlung von Mikroor- C ganismen under the deposit number DSM 4022 on March 3, 1987, and it possesses the following properties: C gram positive, S- forms long coccus chains 0 'c growing temperature: growth at 50 0
C
no growth at 10 0
C
salt resistance growth at a NaCl concentration of 2% c no growth at a NaCl concentration of titrated acidity 25 to 29 OSH after 7-hour cc incubation at 42 0 C (sterilized 10% milk powder milk) lactic acid 0.8% (incubated 2 days at 42 0 C, from milk powder milk) S, fermentates lactose, saccharose and glucose.
Judging from the values given in the prior art, the novel microorganism strain is clearly more sensitive than known Streptococcus thermophilus strains, especially to penicillin and oxytetracycline.
The test set is prepared in the following way: The microorganism strain is grown in a fermentor at a pH of 6.2 to 6.5 and at 38 to 42 0 C in a culture medium based on whey permeate. The growth is observed and the growth is arrested at the end of the logarithmic growth phase, whereafter the culture broth is concentrated by filtrating to a 20-fold concentration. The I A| r I concentrate is measured in a dilution ratio of about 4 to 5 x 10-2, preferably about 5 x 10-2, into a protective agent. The protective agent may consist of water-based protective agents used in the preparation of lyophilized microbe preparations. Preferably the protective agent is an aqueous solution comprising 1.1% of sodium glutamate, 1.1% of ascorbic acid, and optionally 7% of lactose, and the pH of which is The indicator can be added to the protective agent, or c it can be added to the test set in connection with the determination. The indicator is e.g. an acid-base in- O dicator, such as bromocresol purple. The concentrate is measured into a vessel which may be a conventional o ampoule, a sealable test tube, a sample bottle, or the rco like. The vessel is cooled in a carbonic acid-sulphite alcohol bath, whereafter it is lyophilized and stored under vacuum. The finished test set contains about 1 to 2 x 106 bacteria per ml.
The antibiotic determination is carried out by adding a liquid sample and possibly an indicator to the test set. The test set and the sample are incubated and the colour changes are observed. If the C cc sample contains antibiotics, the microorganisms in the test set are not able to grow and the colour does not change. On the other hand, if the sample does not contain antibiotics, the microorganisms grow and induce a colour change while growing.
The sensitivity of the process according to the invention was compared with the corresponding commercial THERMOCULT (Orion Diagnostica) and DELVOTEST P i (Gist-Brocades) techniques and the CHARM II technique.
The sample consisted of milk preheated at 95 0 C for minutes and the determinations were carried out according to the instructions given by the manufacturers. The results are presented in the following i4
I
L :1 6 table, from which further appears the data given by the manufacturer Intervet concerning the INTERTEST.
The results show that the process according to the invention is more sensitive than the other processes and detects clearly the presence of all types of antibiotics/combinations.
c
CIE
I
I
non ftF t
TABLE
Experimentally determined antibiotic sensitivities (pg/ml) of the tested determination processes ANTIBIOTIC PROCESS ACCORDING TO THE THERMOCULT DELVOTEST P INTERTEST b) CHARM INVENTION TEST II A B own deter- a) own deter- a) mination mination PENICILLIN 0.001- 0.001- 0.006-0.0075 0.005- 0.0025 0.0025 0.005 0.003 0.002 I.U. 0.002 I.U. 0.0075 STREPTOMYCIN 1.25 0.25-0.4 5.0 2.5-5.0 5.0 2.5-5.0 5.0 0.1 TETRACYCLINE 0.05-0.1 0.05 0.2 c) 0.1 0.2 0.1 0.5 0.2 OXYTETRACYCLINE 0.1 0.05 0.2 c) 0.1 0.2 0.1 0.2 AMPICILLIN 0.01 0.003 0.01 c) 0.005 0.01 c) 0.005 0.005 ERYTHROMYCIN 0.01-0.05 0.01 0.1 c) 0.5-0.75 0.1 c) 0.75-1.0 0.1 0.01 CHLORAMPHENICOL 0.1-0.5 0.1 1.0 c) 7.5 1.0 c) 7.5-10.0 1.0 0.05 NEOMYCIN 0.5 0.1-0.2 0.5 1.0 1.0 0.5 20.0 STREPTOMAX 0.004 I.U. PEN 0.01 I.U. PEN (penicillin +0.001 pg/ml +0.008 pg/ml +streptom.) STREPTOM. STREPTOM.
MASTALONE 0.1 0.05 0.2 c) (oxytetracycline+the others) A indicator added in connection with the test (duration of the test 4 hours) B indicator added before freeze drying (duration of the test 5 hours) a) Sandstr6m Siveld, 1984, Karjantuote 4 b) concentrations given by the manufacturers c) could not be determined Fz^^ f, j 8 Example 1 Preparation of the test set Bacteria of the Streptococcus thermophilus T101 strain are inoculated in a culture medium having the following composition: of whey permeate powder of casein hydrolysate of tryptone 1% of yeast extract The culture medium has been sterilized at 120 0 C for c to 20 minutes, and its pH is 6.4 after the sterilization.
,cc The test strain is grown in a fermentor at a pH of about 6.2 and at about 42 0 C, and the growth is Smonitored by observing the turbidity of the culture broth. At the end of the logarithmic growth phase the growth is arrested and the culture broth is concentrated by filtrating using a Millipore Pellicon filtration unit (0.45 )um) to a 20-fold concentration, whereby the bacterium concentration of the concentrate is about 2 x 109 bacteria per ml. The concentrate is washed with a small amount of protective agent, and v, O about 5 ml is added to 100 ml of protective agent, whereto is possibly also added 1 ml of bromocresol purple colour (a 0.8% solution). The bacterium concentration of the solution so obtained is about 1 x 108 bacteria per ml. 1 ml of the solution is added to a conventional 10 ml ampoule which withstands drying and can be closed by vacuum. The ampoule is cooled rapidly to 60 s) in a -60 0 C carbonic acid-sulphite alcohol bath, whereafter it is freeze dried and vacuum closed for storage. The ampoule thus prepared contains 1 to 2 x 106 bacteria per ml.
2I E p 1_ Prepraton o th tes se 9 Example 2 Determination of antibiotics in a milk sample Raw milk is heated at 95 0 C for 5 minutes. 2 ml of milk and possibly 20 ,ul of a colour indicator are added to a test set prepared according to Example 1.
The test set is incubated for 4 hours at about 42 0
C
and the colour is evaluated. Milk prepared from sour milk powder and heated at 95 0 C for 5 minutes is used as a control. If the milk contains antibiotics, the colour turns blue. Yellow indicates a negative result.
r C C C C C t C I i c~c

Claims (6)

1. A test set suitable for the determination of antibiotics in milk, characterized in that it comprises a Streptococcus thermophilus T101 concentrate in a water-based protective agent in a dilution ratio of 4 to 5 x 10
2. A test set according to claim 1, characterized in that the protective agent comprises an aqueous solution comprising 1.1% of sodium glutamate, 1.1% of ascorbic acid, and optionally 7% of lactose, and the ph of which is
3. A test set according to claim 1, characterized in that it further comprises an indicator.
4. A test set according to claim 3, characterized in that the indicator is bromocresol purple.
5. Substantially pure culture of Streptococcus thermophilus tl01, dsm 4022 grown on a nutrient medium.
6. A process of determinating antibiotics in milk, characterized by the steps of adding a sample to a test set comprising a Streptococcus thermophilus Ti01 concentrate and a water-based protective agent in a dilution ratio of 4 to x 10- 2 and possibly an indicator, and if the test set does not contain an indicator, an indicator is also added; incubating the test set and the sample at 38 to 42 0 c for about 4 hours; and evaluating the colour. DATED this 28th day of November, 1990. VALIO MEIJERIEN KESKUSOSUUSLIIKE a a 0 Ca. ea€ a ao G o C a a. O 0€ C €COGCC C WATERMARK PATENT TRADE MARK ATTORNEYS "THE ATRIUM", 2ND FLOOR 290 BURWOOD ROAD HAWTHORN VIC. 3122
AU14158/88A 1987-04-07 1988-04-05 A test set and a process for the determination of antibiotics in milk and a novel streptococcus thermophilus strain to be used therein Ceased AU609794B2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FI871512 1987-04-07
FI871512A FI75865C (en) 1987-04-07 1987-04-07 TESTANORDNING OCH FOERFARANDE FOER BESTAEMNING AV ANTIBIOTIKA I MJOELK SAMT I DESSA ANVAENDBAR NY STREPTOCOCCUS THERMOPHILUS-STAM.

Publications (2)

Publication Number Publication Date
AU1415888A AU1415888A (en) 1988-10-06
AU609794B2 true AU609794B2 (en) 1991-05-09

Family

ID=8524275

Family Applications (1)

Application Number Title Priority Date Filing Date
AU14158/88A Ceased AU609794B2 (en) 1987-04-07 1988-04-05 A test set and a process for the determination of antibiotics in milk and a novel streptococcus thermophilus strain to be used therein

Country Status (13)

Country Link
US (1) US4929546A (en)
EP (1) EP0285792B1 (en)
JP (1) JPH072119B2 (en)
AT (1) ATE76437T1 (en)
AU (1) AU609794B2 (en)
CA (1) CA1316442C (en)
DD (1) DD268481A5 (en)
DE (1) DE3871216D1 (en)
DK (1) DK173966B1 (en)
FI (1) FI75865C (en)
IE (1) IE60699B1 (en)
NO (1) NO174898C (en)
NZ (1) NZ223750A (en)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5658790A (en) * 1986-09-04 1997-08-19 Bio 101, Inc. Cell culture media formulated in unit dose
IL91596A0 (en) * 1989-09-11 1990-04-29 Orgenics Ltd Microbiological assay kit and method for detecting antibacterial compounds
DK0611001T3 (en) * 1993-02-11 2002-09-09 Dsm Nv Device for detecting residues of antibacterial compounds in liquids
FR2725212B1 (en) * 1994-09-30 1996-12-20 Gervais Danone Co STREPTOCOCCUS THERMOPHILUS STRAIN, FERMENTATION PROCESS USING THIS STRAIN AND PRODUCT OBTAINED
EP0755456B1 (en) * 1995-02-01 2000-11-08 Dsm N.V. A rapid microbiological test for the detection of antibacterial compounds
FR2732693B1 (en) * 1995-04-06 1997-05-09 Bio Veto Tests Bvt CONTAMINANT REVELATION INDICATOR AND METHOD OF APPLICATION TO THE PRODUCTION OF AN ANTIBIOGRAM DIRECTLY CARRIED OUT ON A SAMPLING
US5827675A (en) * 1995-07-12 1998-10-27 Charm Sciences, Inc. Test apparatus, system and method for the detection of test samples
WO1997003209A1 (en) * 1995-07-12 1997-01-30 Charm Sciences, Inc. Test apparatus, system and method for the detection of test samples
US5792622A (en) * 1995-11-16 1998-08-11 New Mexico State University Technology Transfer Corporation Assay for chemicals
WO2001025471A2 (en) * 1999-10-04 2001-04-12 Dsm N.V. Method for the detection of antimicrobial residues
NZ532039A (en) 2001-10-15 2004-12-24 Dsm Ip Assets B Apparatus and method for detecting undesired residues in a sample
WO2005005656A1 (en) * 2003-07-02 2005-01-20 Dsm Ip Assets B.V. Improved method for the determination of the presence of an antibiotic in a fluid
EP1664330B1 (en) 2003-09-11 2008-11-19 DSM IP Assets B.V. Blood and urine test
US7897365B2 (en) * 2003-11-18 2011-03-01 Charm Sciences, Inc. Method for adjusting antibiotic sensitivity of a test culture
US7824882B2 (en) 2004-06-02 2010-11-02 Dsm Ip Assets B.V. Oligosaccharides in a test system for the determination of the presence of an antibiotic in a fluid
EP1984518A1 (en) 2006-02-08 2008-10-29 DSMIP Assets B.V. Combination of reader and incubator
US8476064B2 (en) * 2006-05-09 2013-07-02 Charm Sciences, Inc. Inhibition assay method and device for detection of antibiotics
WO2011091805A1 (en) 2010-01-29 2011-08-04 Tartu Ülikool (University Of Tartu) On-line system, method of its calibration and simultaneous detection of antibiotic residues and their concentration in milk
WO2012156528A1 (en) 2011-05-19 2012-11-22 Dsm Ip Assets B.V. Method for the determination of the presence of an antibiotic in a fluid
BR112014009194A8 (en) 2011-10-18 2017-06-20 Dsm Ip Assets Bv method for determining the presence of an antibiotic in a fluid
EP2799881A1 (en) 2013-05-02 2014-11-05 DSM IP Assets B.V. Method for the determination of the presence of an antibiotic in a fluid
US9732370B2 (en) 2013-05-02 2017-08-15 Dsm Ip Assets B.V. Method for the determination of the presence of an antibiotic in a fluid
SI3368681T1 (en) 2015-11-20 2019-11-29 Centrient Pharmaceuticals Netherlands B V Assay for determining antibiotics in waste
CN106319023A (en) * 2016-08-19 2017-01-11 浙江工商大学 Method for quickly detecting residual antibiotic in cow's milk or chicken tissues on basis of PGM
CN108676841A (en) * 2018-05-25 2018-10-19 广州南侨食品有限公司 The method of inspection of gemma and heat resistant spores

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU1052288A (en) * 1986-12-02 1988-06-30 Prosciences, Inc. Diagnostic test for staphylococcal mastitis

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3897307A (en) * 1974-10-23 1975-07-29 Hansens Lab Inc Stabilized dry cultures of lactic acid-producing bacteria
DE2930394A1 (en) * 1978-07-26 1980-02-14 Seppo Kolehmainen Determn. of antibiotic concn. in a medium - by observing change in adenosine concn.
US4235964A (en) * 1978-09-28 1980-11-25 Bochner Barry R Method for testing and identifying microorganisms
US4615978A (en) * 1979-06-28 1986-10-07 The State Of Oregon, By And Through The Oregon State Board Of Higher Education On Behalf Of Oregon State University Bacterial growth medium and method of use

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU1052288A (en) * 1986-12-02 1988-06-30 Prosciences, Inc. Diagnostic test for staphylococcal mastitis

Also Published As

Publication number Publication date
FI75865B (en) 1988-04-29
NZ223750A (en) 1990-03-27
AU1415888A (en) 1988-10-06
JPS63269999A (en) 1988-11-08
FI75865C (en) 1988-08-08
NO174898B (en) 1994-04-18
NO880885L (en) 1988-10-10
NO880885D0 (en) 1988-02-29
ATE76437T1 (en) 1992-06-15
NO174898C (en) 1997-11-20
IE60699B1 (en) 1994-08-10
DK117488A (en) 1988-10-08
DK117488D0 (en) 1988-03-04
JPH072119B2 (en) 1995-01-18
FI871512A0 (en) 1987-04-07
EP0285792B1 (en) 1992-05-20
DK173966B1 (en) 2002-03-18
DD268481A5 (en) 1989-05-31
DE3871216D1 (en) 1992-06-25
CA1316442C (en) 1993-04-20
EP0285792A1 (en) 1988-10-12
IE880520L (en) 1988-10-07
US4929546A (en) 1990-05-29

Similar Documents

Publication Publication Date Title
AU609794B2 (en) A test set and a process for the determination of antibiotics in milk and a novel streptococcus thermophilus strain to be used therein
Domig et al. Methods used for the isolation, enumeration, characterisation and identification of Enterococcus spp.: 1. Media for isolation and enumeration
Merlino et al. Evaluation of CHROMagar Orientation for differentiation and presumptive identification of gram-negative bacilli and Enterococcus species
US5786167A (en) Method and culture medium for identification of salmonellae
FI67725C (en) FOERFARANDE FOER FRAMSTAELLNING AV ENHETER AVSEDDA FOER BESTAEMNING AV ANTIBIOTIKA- OCH SULFARESTER I BIOLOGISKA VAETSKOR OCH FRAMSTAELLDA ENHETER
CA2027536C (en) Method for determination of e.coli in water
Neal et al. The use of 2, 3, 5-triphenyltetrazolium chloride as a test for antibiotic substances in milk
CN105713859B (en) Method for detecting multiple antibiotic residues in bifidobacterium breve and milk and application
KR101747347B1 (en) Method for identifying bacteria from the bacillus cereus group
EP1403378B1 (en) Medium for detecting target microbes in a sample
WO1999032656A1 (en) Colorimetric assessment of the sensitivity of helicobacter pylori to antimicrobial substances
Hayward et al. Development of specific tests for rapid detection of Escherichia coli and all species of Proteus in urine
Dalet et al. Evaluation of a new agar in Uricult-Trio for rapid detection of Escherichia coli in urine
US7470520B1 (en) Method for enterobacter identification
Hatcher et al. Radiometric analysis of frozen concentrated orange juice for total viable microorganisms
CN109971669A (en) The detection method and application of bifidobacterium pseudocatenulatum and Residue of Antibiotics in Milk
RU2203317C2 (en) Method of detection of bacillus anthracis in milk
US4229530A (en) Methods and media for rapid detection of pathogenic neisseria
Salter et al. Evaluation of Peel Plate™ EC for Determination of E. coli and Coliform or Total Coliform in Dairy Products
US4952497A (en) Simple and rapid method for detection of virulent Yersinia enterocolitica
US20040241786A1 (en) Single tube screen
Westhoff et al. Detection of penicillin in milk by bioluminescence
ES2755385T3 (en) Liquid nutrient medium for the cultivation of harmful clostridia in dairies and detection procedures
RU2154105C1 (en) Method of quantitative estimation of bifidobacteria content in mixed cultures of microorganisms
JPH0799995A (en) Determination of bifidobacterium bifidus count