AU605839B2 - Compounds having anti-metastatic and/or anti-inflammatory activity - Google Patents

Description

AU-AI-12410/88 PCT WORLD INTELLECTUAL PROPERTY ORGANIZATION 1 t itet rn Mipal Bureau INTERNATIONAL APPLICATION PiBSI D D bE H TE COOPERATION TREATY (PCT) (51) International Patent Classification 4 (11) International Publication Number: WO 88/ 05301 A61K 31/725, 45/05 A l (43) International Publication Date: 28 July 1988 (28.07.88) (21) International Application Number: PCT/AU88/00017 (22) International Filing Date: 22 January 1988 (22.01.88) (31) Priority Application Number: PH 9991/87 (32) Priority Date: (33) Priority Country: 23 January 1987 (23.01.87)

AU

(71) Applicant (for all designated States except US): THE AUSTRALIAN NATIONAL UNIVERSITY [AU/ AU]; Acton, ACT 2601 (AU).

(72) Inventors; and Inventors/Applicants (for US only) PARISH, Christopher, Richard [AU/AU]; 41 Goulburn Street, Macquarie, ACT 2614 SNOWDEN, John, McKinnon [AU/AU]; 4 Duncton Court, Leeming, W.A. 6155

(AU).

(74) Agents: CORBETT, Terence, G. et al.; Davies Collison, 1 Little Collins Street, Melbourne, VIC 3000 (AU).

(54) 1 Cem p HAVING TORY ACTIVITY (81) Designated States: AT (European patent), AU, BE (European patent), CH (European patent), DE (European patent), FR (European patent), GB (European patent), IT (European patent), JP, LU (European patent), NL (European patent), SE (European patent),

US.

Published With international search report.

SThis do ument contains the in:,dincnts made under Section 49 and is correct for printing.

A.O.J.P. 1 5 SEP 1988

AUSTRALIAN

10 AUG 1988 PATENT OFFICE ANTI-METASTATATIC AND/OR ANTI-INFLAMMA- (57) Abstract A method of anti-metastatic and/or anti-inflammatory treatment of an animal or human patient comprises administration to the patient of an effective amount of at least one sulphated polysaccharide which blocks or inhibits endoglycosidase, particularly heparanase, activity. Suitable sulphated polysaccharides include heparin and modified heparin, fucoidan, pentosan sulphate, dextran sulphate and carrageenan lambda.

*'1 WO 88/05301 PCT/AU88/00017 LH9P SiA2PfI IA~ rDE:HAVING ANTI-METASTATIC AND/OR ANTI-INFLAMMATORY ACTIVITY This invention relates to compounds having anti-metastatic and/or anti-inflammatory activity, and in particular it relates to the use of these compounds as anti-metastatic -nd/or anti-inflammatory agents in animals and man.

One of the key events in inflammation and tumour metastasis is the adherence of leukocytes or tumour cells to blood vessel walls and their subsequent emigration into tissues. The molecular basis of these processes is poorly understood although it is generally accepted that penetration of the vessel wall requires localised degradation of the interendothelial cell junctions and subendothelial matrix by specific degradative enzymes.

It has now been discovered that certain sulphated polysaccharides can inhibit tumour cell I 1, metastasis. While some of these sulphated polysaccharides (such as heparin) exhibit anti-coagulant activity, the anti-metastatic activity appears to be unrelated to their anticoagulant activity, the polysaccharides not inhibiting the attachment of tumour cells to vessel walls but preventing vessel wall penetration. Subsequent studies have revealed that the sulphated polysaccharides block tumour cell-derived endoglycosidases that degrade the subendothelial extracellular matrix (ECM) and allow penetration and passage of tumour cells. In particular, these Ssulphated polysaccharides have been found to inhibit the action of heparan sulphate specific glycosidase 15 (heparanase) that degrades the heparan sulphate side-chains of the ECM.

Work leading to the present invention has also revealed that continuous infusion of certain sulphated polysaccharides, such as heparin and fucoidan, can completely prevent experimental alleraic encephalomyelitis (EAE), an animal inflammatory disease similar to multiple sclerosis in humans.

25 n r sp, tpresent invention relates to the use of certain sulphated polysaccharides as anti-metastatic and/or anti-inflammatory agents.

In this aspect, this invention provides a method of anti-metastatic and/or antiinflammatory treatment of an animal or human patient in need of such treatment, which comprises administration to the patient of an effective amount of at least one sulphated polysaccharide which blocks endoglycosidase activity as the sole anti-metastatic and/or anti-inflammatory active agent.

1 j 3 This invention particularly relates to the use of sulphated polysaccharides which block heparinase activity. Suitable sulphated polysaccharides include heparin, fucoidan, pentosan sulphate, dextran sulphate, and carrageenan-lambda.

As previously described, one sulphated polysaccharide which has been found to exhibit endoglycosidase-inhibitory activity is heparin, and in one particularly preferred embodiment of this invention the active component is heparin or a Ge* similar sulphated polysaccharide having I anti-coagulant activity that has been appropriately modified to reduce this anti-coagulant activity.

Examples of such modified polysaccharides are (a) decarboxylated heparin that has a 20 fold reduction in its anti-coagulant activity and negligible loss of anti-metastatic activity and periodate oxidized, reduced heparin that has negligible anti-coagulant activity but is a potent anti-metastatic agent. In both and the S* endoglycosidase-inhibitory activity of the polysaccharides is retained.

The following Example demonstrates that a range of sulphated polysaccharides can inhibit the metastasis of the mammary adenocarcinoma 13762 MAT; _I i i the anti-metastatic activity of the sulphated polysaccharides does not correlate with their anticoagulant activity; and the sulphated polysaccharides do not inhibit adhesion of tumour cells to the vascular endothelium but appear to prevent passaging of tumour cells through the blood vessel wall.

The following Example demonstrates that sulphated polysaccharides block tumour metastasis by inhibiting tumour cell-derived endoglycosidases.

In the Example, hyaluronic acid (which is not a sulphated polysaccharide) is included as a control: o 6 0:S f 0000 I a 0 0 0 S* 0 li- i; WO 88/05301 PCT/AU88/0001,7 MATERIALS AND METHODS EXAMPLE 1 Polysaccharides Hyaluronic acid (Grade III-S from human umbilical cord), chondroitin-4-sulphate (chondroitin sulphate type A from cartilage), chondroitin-6-sulphate (chondroitin sulphate type C from shark cartilage), fucoidan (from Fucus vesiculosus), pentosan polysulphate, carrageenan-kappa (Type III from Eucheuma cottonii), carrageenan-lambda (Type IV from Gigartina aciculaire and Gigartina pistillata) were all purchased from Sigma Chemical Co. (St. Louis, Mo). Heparin (mucous) was supplied by Evans Medical Ltd. (Liverpool, Heparin CSL was obtained from the Commonwealth Serum Laboratories (Melbourne, Australia). Dextran sulphate (2.3 sulphates/monosaccharide, MW 500,000) was purchased from Pharmacia Fine Chemicals (Uppsala, Sweden) and arteparon (Luitpold Werk, Munich, W. Germany) was a generous gift of Dr P. Ghosh, Royal North Shore Hospital (St. Leonards, Sydney, Australia). The polysaccharides, with the exception of Heparin, CSL, which was purchased as a solution, were dissolved in 0.15M NaC1, in most cases to a stock concentration of 20 mg/ml. Hyaluronic acid and the carrageenans because of their viscosity in solution were dissolved in 0.15M NaCI to concentrations of 10 mg/ml and 2 mg/ml respectively. All polysaccharide solutions were stored at -200.

Animals and cell lines Female Fisher F344 inbred rats were bred at the John I wo 88/05301 PCT/AU88/00017 6 Curtin School of Medical Research and used at 10 weeks of age.

The 13762 MAT cell line is a mammary adenocarcinoma of Fisher 344 rats adapted to in vitro culture in RPM1 1640 medium (Gibco) supplemented with 10% foetal calf serum (FCS; Flow Labs), 100 units/ml penicillin and 100 ug/ml streptomycin sulphate as described previously These cells are highly metastatic and exhibit stable metastatic properties over a number of passages in culture.

Haematogenic metastases assay 13762 MAT cells were dislodged from the surface of tissue culture bottles by vigorous shaking, the cells were then washed and resuspended in complete medium. 2x10 viable cells in 0.6 mis were injected into the tail vein of Fisher 344 rats. Twelve days after injection the animals were killed, the lungs removed and fixed in Bouins fluid and the number of surface metastatic foci determined. By this injection route metastases are confined to the lung.

Soft agar plating The plating of cells in soft agar was performed essentially as described by Reid Briefly, an underlayer consisting of 2 mis of 0.5% Difco Bacto-agar in 1640 medium 2containing 10% calf serum was poured into 60 mm petri dishes (Sterilin, Teddington Middlesex) and allowed to solidify at for 1h. The cells to be plated were suspended in 0.33% agar in 1640 medium and 10% calf serum and 6 mis of this WO 88/05301 PCT/AU88/00017 7 mixture was poured over the underlayer. The plates were first placed at 4 0 C for 1h to allow the agar to solidify and then incubated at 370 in a humidifed 5% C02 atmosphere for 14 days. Colonies are visible after 7-10 days at 370 and can be scored at 14 days.

Rosetting assay for cell surface receptors for sulphated polysaccharides Rosetting assays were carried out in 96-well roundbottomed microplates (Linbro Chemical Co.) and based on a procedure reported earlier 13762 MAT cells were washed and resuspended in phosphate buffered 0.15M NaC1 (pH 7) supplemented with 0.1% bovine serum albumin (PBS/BSA). To 25 ul of ice cold 13762 MAT cells (1x10 6 /ml in PBS/BSA) was added 25 ul of a 17 suspension in PBS/BSA of either washed sheep erythrocytes or sheep erythrocytes coupled with a sulphated polysaccharide using CrC13 as previously described This mixture was pelleted by centrifugation at 1,000 rpm for 1 min 2at 40 and left on ice for 1h to allow the rosettes to stabilize. The cell pellets were then gently resuspended with a short Pasteur pipette and stained with Methyl violet; ul of 0.057 methyl violet being added to the wells. Cell samples were transferred to a haemocytometer chamber and the percentage of rosette-forming cells assessed. A minimum of 100-200 tumour cells were examined for rosettes. A tumour cell with 6 or more erythrocytes attached was considered as a rosette.

S WO 88/05301 PCT/AU88/00017 8 Labelling 13762 MAT cells with Hoechst dye No. 33342 Hoechst dye No. 33342 (H33342; Calbiochem-Behring, Kingsgrove, NSW, Australia) was stored at 40 as a stock solution of 600 ug/ml in distilled water. For labelling, 13762 MAT cells were washed and resuspended to a concentration of 5x10 7 cells/ml in RPM1 1640 medium supplemented with 10% FCS. The cells were transferred to a 370 water bath and 6 ug/ml of H33342 was added. Labelling was continued for 15 min after which the cells were washed twice with cold RPM1 1640 medium and resuspended for injection.

Quantification of tumour cell lodgement The method used was, similar to that of Brenan and Parish 13762 MAT cells (2x106) were injected i.v. into rats, in 0.6 ml of RPM1 1640 medium containing either 200 units of CSL heparin (ca 1.6 mg) or 4 mg of chondroitin-4sulphate. Labelled cells injected in RPM1 1640 alone served as controls. At various times after injection the rats were anesthetized, bled by cardiac puncture and the lungs were removed, washed and placed in saline. Each lung was then minced in PBS and made into a single-cell suspension by gently pressing the tissue fragments through a fine seive.

y The cell suspension was centrifuged, washed with PBS and resuspended in 4 ml of PBS. A haemocytometer was used to estimate the number of fluorescent cells within each lung.

At least 3 animals were used for each timepoint and each treatment.

-a.

WO 88/05301 PCT/AU88/00017 9 Anticoagulant and procoagulant assays To obtain rat plasma blood was collected by the cardiac puncture of anesthetized rats; nine vols of rat blood being drawn into one volume of 3.8% sodium citrate. The erythrocytes were removed by centrifugation (10,000 g, 4°C) and the plasma collected and stored at -700 until use.

The effect of the sulphated polysaccharides on the coagulation state of plasma was determined as follows.

Polysaccharide, 100 ul diluted in 0.15M NaC1 was mixed with 50 ml of normal plasma and 50 ul of 0.15M NaC1 and the mixture was warmed at 370C for 2 mins. To activate the coagulation pathway either 20 ul of bovine thrombin (Sigma) at a concentration of 30 NIH units/ml in 1.5M NaCl or 20 ul of activated "Thrombofax" reagent-optimized (partial thromboplastin with activator, Ortho Diagnostic Systems Inc.) was added to the mixture. The clotting reaction was then initiated by the addition of 100 ul of 30 mM CaC1 2 and the time, in seconds, required for clot formation was recorded.

These values were compared to the time taken for plasma to clot in the absence of sulphated polysaccharides, when the 100 ul of polysaccharide was replaced with 100 ul of saline. The highest concentration of polysaccharide having no detectable effect on the clotting time was taken as the endpoint. The addition of thrombin or "Thrombofax" was necessary to remove the variability introduced by the incomplete activation of the intrinsic pathway that resulted when surface-contact was the only agent activating the coagulation cascade.

WO 88/05301 PCT/AU88/00017 A similar assay was used to determine the effect of sulphated polysaccharides on the procoagulant activity of the 13762 MAT cells. To 100 ul of normal rat plasma was added ul of polysaccharide in,0.15M NaCl.and 50 ul containing 2x10 4 13762 MAT cells in 1640 medium (no serum). After warming for 2 min at 370 100 ul of 30 mM CaCI2 was added and the time (seconds) for clot formation was measured. The clotting time of plasma when the coagulation cascade was activated by 2x10 4 13762 MAT cells in the absence of polysaccharide (the 50 ul of polysaccharide was substituted with 50 ul of saline) served as the control. The highest concentration of polysaccharide that does not produce a detectable increase in the clotting time above that recorded for the controls was the end point. For both assays the effect of each polysaccharide concentration was measured in duplicate and the control values were determined from the mean of at least eight clotting times.

WO 88/05301 PCT/AU88/00017 11 FIGURE 1 Lodgement pattern within the rat lung of i.v. injected, H33342 labelled, MAT cells. The cells were injected in either saline alone (A and C) or saline containing 1.6 mg heparin (B and The pattern of 13762 MAT cell lodgement is shown 15 min (A and B) and 360 min (C and D) after injection. Magnification FIGURE 2 Quantitative assessment of the effect of sulphated polyaccharides on the lodgement of H33342 labelled, 13762 MAT within the rat lung over a 22h period. Experiment 1: Cells were injected i.v. in saline alone or saline containing 1.6 mg of heparin A Experiment 2: Cells were injected 3.v. in saline alone Q or saline containing 4 mg of chondroitin-4sulphate The mean and standard error of the number of fluorescent tumour cells within at least 3 replicate rat lungs is given.

OIL.r- S WO 88/05301 PCT/AU88/00017 12 i RESULTS i i Inhibition of metastasis with sulphated polysaccharides To test whether sul.phated polysaccharides could alter the number of 13762 MAT cell lung metastases the following experiment was performed. Single cell suspensions of 13762 MAT cells (2x10 5 were mixed with 4 mg of sulphated polysaccharide in RPM1 1640 medium immediately prior to their injection into the tail vein of rats. Twelve days after injection the numbers of visible surface lung lesions were determined. Although a score of the number of visible lesions does not represent the total number of tumours within the lung it is regarded as a reliable estimate of the extent of metastatic tumour colonization It is clear from Table I that certain sulphated polysaccharides substantially decreased the number of lung lesions. Heoarin was the most effective polysaccharide followed by carrageenan lambda, pentosan sulphate and fucoidan.

It was necessary to eliminate the possibility that the apparent antimetastatic effects of the sulphated polysaccharides were caused by their direct toxicity for tumour cells.

20 Accordingly, samples of 13762 MAT cells were incubated for 1h at S370 with one of each of the sugars shown to inhibit metastasis.

After incubation the cells were washed and plated in soft agar.

The concentration of cells and sugars was the same as that used for injecting the rats i.e. 3.3x10 5 cells/6.6 mg sugar/ml or 3.3x10 5 cells/3.3 mg sugar/ml in the case of the carrageenans.

Cells incubated in RFM1 1640 medium alboe served as controls. The effect of the sugars on the viability of the tumour cells was -1C WO 88/05301 PCT/AU88/00017 13 assessed from the number of cell colonies visible at 14 days after plating. Dextran sulphate was the only sugar found to reduce either the size or the number of 13762 MAT cell colonies (Table II); the other sugars had no detectable effect on tumour cell viability in vitro.

This suggests, with the exception of dextran sulphate, that the reduction in the number of metastases was a consequence of some in vivo action of the polysaccharides. To determine the validity of this interpretation heparin was administered independently of the i.v. injected tumour cells. It was found that the route of heparin injection, whether intraperitoneal, subcutaneous or via another tail vein, did not alter the result.

Heparin continued, in each case, to decrease the number of metastases to less than 10% of the control (data not shown); thus, confirming that heparin, at least, was acting in vivo to reduce the number of metastases.

Detection of receptors for sulphated polysaccharides on 13762 MAT cells Sulphated polysaccharides constitute a major component of the extracellular matrix of endothelial cells, hence it is possible that 13762 MAT cells may adhere to the lung endothelium via receptors for these molecules. To determine whether molecules associated with the surface of 13762 MAT cells bound sulphated polysaccharides a rosetting assay was used.

Sulphated polysaccharides from a variety of sources were coupled to the surface of sheep erythrocytes and the ability of these erythrocytes to attach to 13762'AT cells was assessed.

rrmn~- ra~-T WO 88/05301 PCT/AU88/00017 14 Uncoupled sheep erythrocytes served as controls. It was found that erythrocytes coupled with the glycosaminoglycans (GAG) Schondroitin-4-sulphate and chondroitin-6-sulphate bound strongly to the surface of 13762 MAT cells while those coupled with hyaluronic acid (a nonsulphated GAG) bound moderately; 77% of the S13762 MAT cells being classified as rosettes (Table III). In contrast, arteparon (an artificially oversulphated GAG from bovine lung) and heparin-coupled erythrocytes bound very poorly to 13762 SMAT cells. A similar pattern of selective adhesion was displayed 0by 13762 MAT cells for erythrocytes coupled with sulphated Spolysaccharides from non-mammalian sources. Although the carrageenans kappa and lambda bound very strongly to 13762 MAT cells a subpopulation of these cells (ca 32%) consistently did not bind carrageenan lambda. No binding of pentosan sulphate-coupled erythrocytes could be detected and only a subpopulation of 13762 MAT cells (ca 50%) bound rather weakly to dextran sulphate-coupled erythrocytes.

The selective nature of the binding pattern displayed by the 13762 MAT cells indicates that binding is not simply due to the anionic nature of the polysaccharides. For example, the tumour reacted strongly with the chondroitin sulphates and yet did not bind heparin a GAG with a much higher charge density.

SSimilarly, hyaluronic acid, a molecule having a relatively low charge density was found to adhere quite strongly to 13762 MAT I cells. It can therefore be concluded that 13762 MAT cells possess 2 5 surface associated molecules (receptors) that bind particular sulphated polysaccharides. There was, however, no positive correlation between the antimetastatic properties of the sugars and their ability to bind to the tumour cell surface (Tables I and III). In fact, with the exception of carrageenan lambda, a l iII WO 88/05301 PCT/AU88/00017 negative correlation was evident. This suggests that the antimetastatic activity of the majority of the sulphated polysaccharides could not have been due to the blocking of sulphated polysaccharide -specific 'receptors on the 13762 MAT cell surface.

Anticoagulant activity of the sulphated polysaccharides 13762 MAT cells are known to exhibit procoagulant activity in vitro -a property believed to contribute to the metastatic capability of tumour cells by increasing the probability that these cells will become entrapped in the microcirulation of an organ The sugars 'most effective as antimetastatic agents were found, at quite low concentrations, to both exhibit anticoagulant activity and inhibit the procoagulant activity of the 13762 MAT cells (Table IV). Nevertheless, it is noteworthy that the correlation between antimetastatic and anticoagulant activity is not absolute.

Dextran sulphate, for example, inhibited the clotting of plasma at concentrations as low as 1-0.5 ug/ml as did carrageenan lambda 2 (Table IV) yet carrageenan lambda was substantially more effective at preventing metastasis than dextran sulphate (Table I).

Moreover, dextran sulphate was found to impair the viability of the 13762 MAT cells (Table II) thus, it is probable that the reduction in metastasis observed with this sugar was a reflection of its toxicity. Pentosan sulphate and arteparon similarly exhibited identical endpoints in the anticoagulant assays but differed significantly in their efficacy as antimetastatic agents.

It is worth noting that the anticoagulant and procoagulant assays WO 88/05301 PCT/AU88/00017 16 give accurate end points as a narrow range of sugar concentrations can alter plasma from giving coagulation times identical to that of the control to an incoagulable state (data not shown).

Effect of sulphated polysaccharides on the arrest of tumour cells in the lung Do the sulphated polysaccharides that reduce metastasis prevent the-arrest and/or adhesion of tumour cells to the lung endothelium or do they act at a later stage in the metastatic process? To examine this question the effect of heparin and fucoidan on the arrest, in the lung, of 13762 MAT cells labelled with a fluorescent dye (H33342) was determined. This dye has been used previously to follow lymphocyte recirculation and is reported neither to be toxic nor to modify the cell surface Fluorescently.labelled 13762 MAT cells (2x10 6 were injected i.v. in either saline or with fucoidan (4 mg) or heparin (4 mg and 1.6 mg). At 15, 90 or 360 min after injection the rats were bled by cardiac puncture, then killed and the lungs were removed. After washing the lungs were fixed in 5% neutral 20 formalin for 20h at room temperature. Hand sections of the fixed tissue were examined using low power (100x) fluorescence microscopy. In all cases the cells exhibited a patchy distribution spread throughout the lobes of the lung (Fig. la and b) and no qualitative difference in the number of cells arresting after 15 min could be detected. However, the numbers of cells visible in the lung 6h after injection had declined substantially when heparin or fucoidan had been.administered (Fig. 1c and d).

SWO 88/05301 PCT/AU88/00017 17 In a subsequent experiment the effect of sulphated polysaccharides on the arrest and lodgement of 13762 MAT cells in Sthe lung was quantified over a 22h period. Tumour cells labelled with H33342 were injected with either a sugar, heparin (1.6 mg) or chondroitin-4-sulphate (4 mg), or in RPM1 1640 alone, and at the times specified (Fig. 2) the numbers of labelled cells remaining in the lungs were estimated. Plasma prepared from the blood samples taken at each time point was used to monitor the anticoagulant state of the rats. It was found that 1.6 mg of heparin significantly anticoagulated the animals and inhibited the procoagulant activity of the tumour cells for between 3-5 hours.

Plasma taken from rats 5h after the injection of heparin exhibited a clotting pattern indistinguishable from that of normal plasma.

Chondroitin-4-sulphate had no effect on the coagulation state of the rats.

The results of this experiment confirmed the qualitative assessment. Heparin did not prevent the initial arrest of the tumour cells but it did increase the rate at which these cells were lost from the lung, such that, after 22h only 38% of the cells initially arrested could be detected. In contrast, i chondroitin-4-sulphate, a sugar having no anticoagulant and no antimetastatic activity, had no effect on the retention of tumour cells in the lung (Fig. The displacement of cells from the lung observed with both heparin and fucoidan is thus not due simply to the introduction of a sulphated polysaccharide per se but appears to be more specific. Whether the displacement of cells is due to the anticoagulant effect of the sugars is not clear. However, the data do suggest that the procoagulant activity of the 13762 MAT cells is of little consequence for the L i *n

J

J* 'n i- WO 88/05301 PCT/AU88/00017 18 initial steps of tumour cell arrest, as heparin and fucoidan are potent inhibitors of procoagulant activity (Table IV).

Effect of the time of heparin administration on its antimetastatic activity The effects of heparin on the number of 13762 MAT cells remaining in the lung first became evident 1-2 hours after injection (Fig. Thus, it could be argued that heparin interferes with the metastatic process after the cells have lodged 10 in the lung capillaries but before penetration of the vascular endothelium. To determine the time of heparin administration most effective in preventing metastases, heparin was given both before and after the tumour cells and the resulting number "of lung lesions were recorded.

Heparin most efficiently inhbited metastasis formation when an i.v. injection of tumour cells was immediately followed by an injection of heparin into a different tail vein. However, around inhibition of metastasis could still be achieved if heparin was given up to one hour before or three hours after the tumour cells (Table As before the rats were significantly anticoagulated three hours after the heparin injection but after six hours their coagulation state had returned to that of the saline injected controls (data not shown).

Separation of the antimetastatic and anticoagulant effects of heparin From the data presented above it appears that anticoagulation WO 88/05301 PCT/AU88/00017 19 may not be the complete explanation for the antimetastatic effects of the sulphated polysaccharides. Commercial preparations of sulphated polysaccharides are composed of a heterogeneous set of molecules, different preparations of the same polysaccharide having a slightly different set of molecules. It is therefore possible that heparin batches could vary in their potency as antimetastatic agents, yet possess identical anticoagulation properties. This was found to be the case. Heparin preparations from two different sources had identical anticoagulant properties but differed by approximately 10-fold in their antimetastatic capabilities (Table VI). The quantity of Evans Medical Ltd heparin and CSL heparin required to drop the number of lung metastases by 50% was 0.53 mg and 0.06 mg respectively. These results indicate that the antimetastatic effect is due, at least in part, to some component of heparin distinct from that required for anticoagulation.

-r

-I

WO 88/05301 PCT/AU88f00017 TABLE I THE EFFECT OF SULPHATED POLYSACCHARIDES ON THE METASTASES FORMED BY 1 3762 MAT CELLS No M~etastases Polsachriel(Mean

SE)

2 of control Hyaluronic acid (or\)295 31 100.1 Chondroitin-4-sulphate 285 27 96.5 Chondroitin-6-sulphate 273 36 9'2.5 Heparin 30 10 10.2* Fucoidan 96 21 32-7* Carrageenan Kappa 282 29 95.6 CarrageenarvLambda 56 13 18.9* Dextran sulphate 151 21 51.1* Pentosan sulphate 65 13 22.0* Arteparon 139 25 47.3* None (control) 295 lFour mg of polysaccharide were injected per rat with the exception of both the carrageenans, where 2 mg were injected.

2 Mean of 5 replicates.

*Significantly different from the control as determined by an analysis of variance followed by an a priori t-test In each case p K 0.001.

V~'

WO 88/05301 PCT/AU88/00017 TABLE II EFFECT OF SULPHATED POLYSACCHARIDES ON THE VIABILITY OF 13762 MAT CELLS No. of MAT cell colonies Polysaccharide' R SE Heparin 333 39 Fucoidan 330 32 Carrageenan Kappa 379 12 Carrageenan Lambda 305 2 Dextran sulphate 172 27 Pentosan suLphate 322 3 None (control) 310 +'7 13762 MAT cells were incubated for 1h at 370 in polysaccharide (3.3 mg/ml for the carrageenans Kappa and Lambda; 6.6 mg/ml for the others) in 1640 medium before being added to agar and plated out. Four replicate plates were prepared for each sugar.

I

WO 8805301PCT/AU88/ 000 17 2 2 TABLE III -RECEPTOR STATUS OF 13762 MAT CELLS FOR SULPHATED POLYSACCHARIDES of MAT cells Polysaccharidel forming rosettes 2 Hyaluronic acid 77 Chonidroitin-4- sulphate 98 Chcndroitin-6,-su iphate 74 H epar in <1 Fucoidan Carrageenan Kappa 92 Carrageenan Lambda 7.8 Dextran sulphate 53 Pentosan sulphate <1 Arteparon <1 None (control) <1 lPolysaccharides were coupled to sheep erythrocytes.

213762 MAT cells possessing 6 or more attached erythrocytes constitute a rosette. Figures-given are the average of three separate experiments.

WO 88/05301 WO 8/05301P CT/A U88/000 17 TABLE IV EFFECT OF SULPHATED POLYSACCHARIDES ON OF RAT PLASMA THE COAGULATION STATE Antico agulant a'~tivity (ug/ml), inhibition of procoagulant effect Polysaccharide of MAT cells (ug/ml) 2 APTT TT Hyaluronic acid Ccockzxa\) >2000 >2000 >2000 Chondroitin-4-sulphate 500 >5000 >2000 Chondroitin-4-sulphate 500 >5000 >2000 Heparin 0.3 0.3 3 Fucoidan 1.0 1.0 Carrageenan Kappa 3.0 10.0 200 Carrageenan Lambda 1 .0 0.3 Dextran sulphate 0.3 0.3 2 Pentosan sulphate 3.0 3.0 Arteparon 3.0 3.0 lHighest concentition, of sugar having no detectable effect on plasma coagulation.

2 Highest concentration of sugar having no inhibitory effect on the procoagulant activity of 2xj0 4 13762 MAT cells.

WO 88/05301 WO 8805301PCT/AU88/000 17 TABLE V EFFECT OF TIME OF HEPARIN ADMINISTRATION ON ITS XNTTItETASTATIC ACTIVITY Time (hours) of injection Number of M~etastases 13762 MAT Heparin 2 (mean SE) 3 of control cells 1 O 0 7 +3 2.4 0 +1 21 +4 7.1 0 +3 73 9 25.0 0 +6 202 68 69.1 0 +22 248 48 85.2 0 No sugar (control) 291 39 0 0 25 7 9.7 +0 0 82 26 31.9 +3 0 106 6 41.7 +6 0 166 47 65.3 +22 0 272 66 106.8 0 No sugar (control) 254 51 1 2x10 5 cells injected i.v.

2 Each rat received 1 .6 mg Heparin i.v.

3MIean of at least 3 replcates.

-I I II i hi' WO 88/05301 2 PCT/AU'88/00017 TABLE VI COMPARISON OF THE ANTIMETASTATIC AND ANTICOAGULANT ACTIVITY OF TWO HEPARIN BATCHES No. of metastases of control) 1 with: Quantity of heparin injected (mg/rat) Evans Medical Ltd heparin CSL heparin 1.6 13.9 6.3 10.9 2.2 0.53 45.8 10.8 1-8.5 9.4 0.18 125 20.1 24.4 6.2 0.06 135 16.6 50.8 3.9 0.02 ND 71.8 10.7 Anticoagulant activity (ug/ml) 2

APTT

3 0.3 0.3

TT

4 0.3 0.1 1 Mean standard error calculated from at least 3 replicates.

2 Highest concentration of heparin having no detectable effect on plasma coagulation.

3 Activated partial thromboplastin time test.

4 Thrombin time test.

ND not determined.

c I S WO 88/05301 PCT/AU88/00017 26 The following Example demonstrates that sulphated polysaccharides block tumour metastasis by i inhibiting tumour cell-derived endoglycosidases.

EXAMPLE 2 Table VII presents results from endoglycosidase-inhibition experiments which demonstrate that there is a correlation between the endoglycosidase inhibitory activity of the different sulphated polysaccharides and their ability to inhibit tumour metastasis. Thus, the five polysaccharides that exhibited anti-metastatic activity were comparable inhibitors of tumour cell derived endoglycosidases. In contrast, of the four polysaccharideS that failed to inhibit tumour metastasis, three had no detectable endoglycosidase inhibitory activity and one polysaccharide (carrageenan-kappa) was approximately 4-7 times less effective at inhibiting the tumour endoglycosidases as the anti-metastatic polysaccharides.

Although experiments described in Example 1 above suggest that the anticoagulant activity of the sulphated polysaccharides plays little or no role in their antimetastatic activity, it was important to obtain more direct evidence that this is indeed the case. Table VIII presents the results of an experiment in which heparin was separated into anticoagulant enriched and anticoagulant depleted fractions by passage over an anti-thrombin III column (heparin exerts its anticoagulant activity by interacting with anti-thrombin III in plasma).

Approximately 40% of the heparin preparation used in this experiment bound to the anti-thrombin III column and was eluted with 2M NaC1 (termed high affinity rl 1 W 0 88/0530 t PCT/AU88/00017 27 heparin). It was found that the heparin fractions with high and low affinity for anti-thrombin III had identical endoglycosidase-inhibitory activity, almost i identical anti-metastatic activity (comparable to unfractionated heparin) but differed approx. 300-500 fold in their anticoagulant activity. Such a result clearly indicates that the anticoagulant activity of heparin plays little or no role in the j anti-metastatic properties of the polysaccharide.

In additional experiments attempts were made to chemically modify heparin such that the anticoagulant activity of the polysaccharide was destroyed but the anti-metastatic activity of the molecule retained. Such procedures eliminate the undesirable anticoagulant properties of heparin if it is to be used clinically as an anti-metastatic and anti-inflammatory drug and (ii) unlike anti-thrombin III fractionation, provide a commercially viable approach for preparing an effective drug. The results obtained with two chemically modified preparations of heparin, which are virtually devoid of anticoagulant activity, are presented in Table IX. Both preparations exhibited substantial anti-metastatic activity although they were less effective than unmodifed heparin.

WO8/-50 28 PCT/AU88/00017 TABLE VI I Ability ofDifferent Sulphated Polysaccharides to Inhibit Tumour Cell Derived Endoglycosidases Inhibits Endoglycosidase Polysaccharide Tumour Metastasis Inhibitory Activity Hyaluronic acid LnUr1) Chondroitin-4-sulphate Chondroitin-6--sulphate Carrageenan-kappa Carrageenan-lambda Dextran sulphate 1.6 Fucoidan 2.8 Pentosan sulphate 2.4 Heparin 'Concentration of polysaccharide required to produce a inhibition of degradation of the extracellular matrix so S 4 -labelled) of endothelial cells by 13762 MAT cells.

J-

TABLE VIII 0- Biological Activity of Heparin Fractions with High and Low Affinity for Anti-Thrombin III 00 Anticoagulant Activity Endoglycosidase Number of Heparin (pg/ml) 1 Inhibitory Activity metastases Fraction APTT TT (pg/ml) 2 control) 3 Unfractionated 0.25 1.5 1.5 6.3% High affinity 0.13 0.75 2.3 7.7% heparin 4 .Low affinity 62 250 2.0 17.6% heparin 1 Highest concentration of polysaccharide having no detectable effect on plasma coagulation. APTT activated partial thromboplastin time test, TT thrombin time test.

2 Concentration of polysaccharide producing a 50% inhibition of degradation of the extracellulac matrix of endothelial cells by 13762 MAT cells.

3 5 2 x 10 MAT cells injected i.v. into each rat with 2 mg of each heparin fraction and number of lung metastases -o quantified 12 days later.

4 Heparin separated into fractions with high and low affinity for anti-thrombin III by passage through an C anti-thrombin III coupled column. 00 o

®C)

i

Y

wp~ uK WO 88/05301 PCT/AU88/00017 i i H Preparation of Chemically Modified Heparins i Heparin was periodate oxidized and potassium borohydride reduced based on the method of Fransson Heparin (10 mg/ml, bovine lung) in 50mM sodium phosphate buffer, pH 7.0, containing 40mM sodium periodate was incubated at 37 0 C in the dark for hr. The reaction was stopped by the addition of D-mannitol (5 mg/ml). Low molecular weight reaction products were removed by dialysis against distilled water. The oxidized heparin was then reduced by the addition of 20 mg/ml of KBH 4 and incubation for 3hr at 200C. Excess borohydride was decomposed by the addition of 20p1/ml of glacial acetic acid. The oxidized and reduced heparin was precipitated twice at 4 0 C by the addition of 2 vols ethanol and finally redissolved in 0.15 M NaCI.

N-desulphated heparin was prepared by heating the material in 0.04 M HC1 at 1000C for min. N-acetylated heparin was obtained by treatment of N-desulphated heparin with acetic anhydride as previously described

I

WO 88/05301 XVO 8/050 1PCT/AU88/00017 TNBLE I X Anti-Metastatic Activity of Chemically Modified Heparins with Negligible Anticoagulant Activity Polysaccharide' Number of Metastases (mean SE) of control N-acetylated 158 24 43.8 heparin No polysaccharide 360 54 (c~n tr 01) Periodate-oxidised 214 35 43.6 he par in Unmodified heparin 95 10 19 No polysaccharide 491 (control) 1'Chemically modified heparins had anticoagulant activity of unmodified heparin.

2 etastasis experiment as in Table VIII S WO 88/05301 PCT/AU88/00017 32 The following Example demonstrates the r Vi anti-inflammatory activity of the sulphated !i polysaccharides, heparin, fucoidan and pentosan i sulphate.

5 EXAMPLE 3 j MATERIALS AND METHODS Rats. Lewis (RT-1) rats were bred at the John Curtin School of Medical Research. Both males and females of 8-10 weeks of age were used. In each experiment controls and experimental rats were matched for sex.

WO 88/05301 PCT/AU88/00017 33 Induction of EAE.

Active. Guinea pig BP was prepared according to the method of Deibler et al (11) and BP in saline was emulsified in an equal volume of incomplete Freund's adjuvant containing 4 mg/ml added Mycobacterium butyricum. Rats received 0.1 ml emulsion in one footpad of both hin'd feet.

Total dose received was 50 Ag BP and 400 s.g Mycobacterium butyricum.

Passive. Cells for passive transfer were generated following the method of Panitch and McFarlin (12) Single cell suspensions were prepared from spleens of donor rats sensitized 12 days previously with BP-CFA as described above. Cells were cultured at 2x10 6 /ml in RPMI 1640 fetal calf serum, 5xl0-5M 2-mercaptoethanol, 200 mM Lglutamine and'penicillin and streptomycin. Con A was added at 2 Ag/ml and cultures were incubated at 37 0 C in an atmosphere of 10% CO2, 7% 02 and the balance N 2 Cells were harvested after 72 hrs, washed with Hanks balanced salt solution (BSS) and transferred to recipients via a lateral tail vein. All transfer populations contained 30x10 6 viable cells.

i -e WO 88/05301 34 PCT/AU88/00017 Evaluation of clinical EAE Clinical EAE was graded according to the following scheme: 0, asymptomatic; 1, flaccid distal half of tail; 2, entire tail flaccid; 3, ataxia, difficulty in righting; 4, hind limb weakness; 5, hind limb paralysis.

In most experiments we also calculated the mean day of onset of disease (MDO), the mean clinical score (MCS) and the mean length or duration of disease (MLD). Values are expressed standard error of the mean.

Histology Rats were perfused with 10% neutral buffered formalin.

Their spinal cords removed and prepared by standard histological techniques. Slides were stained with H and E.

Polysaccharides Chondroitin-4-sulfate, fucoidan (from Fucus i vesiculosus), pentosan polysulfate and heparin (sodium salt from bovine lung) were all purchased from Sigma (St. Louis, MO). The polysaccharides were dissolved in 0.15 M NaC1 and stored at -20 0 C. They were thawed and then boiled for 2 min immediately before use.

WO 88/05301 35 PCT/AU88/00017 j Heparin devoid of anticoagulant activity was prepared by periodate oxidation and borohydrate reduction using a similar procedure to that described by Fransson 9).

Bovine lung heparin (10 mg/ml) was dissolved in 0.05 M sodium phosphate buffer, pH 7.0, containing 40 mM sodium periodate and left to react for 18 hrs at 37 0 C in the dark.

The reaction was stopped by the addition of solid D-mannitol mg/ml) and the solution then dialyzed against distilled water at room temperature for 2 hrs, the dialysate being changed every 30 min. The oxyheparin was then reduced by the addition of solid potassium borohydride (2 mg/mg heparin), the reduction reaction being left for 3 hrs at room temperature and then terminated by the addition of glacial acetic acid (1 u]/mg of borohydride). The heparin was then ethanol precipitated twice (2 vols ethanol, 4 0

C,

18 hrs) and finally dissolved in 0.15 M NaC1 to a concentration of 20 mg/ml. Approximately 50% of the heparin was recovered as the periodate oxidised borohydride reduced preparation.

The anticoagulant activity of heparin and periodate oxidized heparin was determined using rat plasma in the activated partial thromboplastin time and thrombin time Stests Based on these assays periodate oxidation resulted in a 500-2000 fold reduction in the anticoagulant activity of heparin.

*n a i i r ii WO 88/05301 PCT/A1J88/00017 Delivery of sulfated polysaccharides. Because of the short half life of some of the polysaccharides in vivo we thought it necessary to give repeated doses. We first attempted ip injections of heparin every twelve hours.

Unfortunately, this produced unacceptable levels of hemorrhage and even death of some animals. We chose therefore to use mini osmotic pumps (type 2ML, ALZA Corp, Palo Alto, Calif.) which were implanted subcutaneously in the back. The pumps have a 2m1 capacity and deliver approximately 10 Al/hr for 7 days. Plasma levels of heparin was measured in rats implanted with an osmotic pump containing 20 mg/ml. The method employed was essentially the dimethylmethylene blue procedure of Farndale et al (13).

A steady state concentration of 10-20 4g/ml was reached by 24 hrs after implanting the pump on day 0 and no heparin was detectable on day 8, i.e. 24 hrs after the pump ceased to deliver.

RESULTS

Recipient rats received 30x10 6 EAE effector cells and at the same time osmotic pumps were placed subcutaneously in the back. The pumps contained 2 ml of heparin at either mg/ml or 20 mg/ml. As shown in Table X there was a degree of protection in both heparin treated groups. Only 3 of 6 rats receiving 10 mg/ml heparin developed disease and only 1 of 5 receiving 20 mg/ml. In the latter case, the one animal WO 88/05301 PCT/AU88/00017 which did develop disease did so later after cell transfer and also exhibited a milder disease.

In the next experiment we used heparin again as well as three further sulfated polysaccharides, fucoidan, chondroitin-4-sulfate, 'and pentosan sulfate, and also asked if initiation of treatment could be delayed for 3 days and still provide protection. Fucoidan as well as heparin gave complete protection against EAE even when treatment was delayed till day 3 after cell transfer (Table Pentosan sulfate gave partial protection as evidenced by later onset, milder clinical disease, and shorter duration of disease.

Chondroitin-4-sulfate had no protective effect.

Often, therapeutic studies of various agents in EAE demonstrate that clinical disease may be abrogated, yet histopathologic examination will reveal quite extensive inflammatory lesions (14, 15). To examine this in the present context, 3 control and 3 heparin treated animals from the experiment described in Table XI were killed on day 8 post cell transfer and examined for inflammatory lesions.

Virtually every low power field of a 2 cm longitudinal section through the lower thoracic/upper lumbar cord of control rats had numerous perivascular inflammatory lesions.

In contrast, no lesions were seen in any of sections from the same area of the three heparin treated rats.

1:I-

V

7i WO 88/05301 PCT/AU88/00017 To determine if the sulfated polysaccharides are inhibiting adoptive EAE by simply killing the transferred cells, we examined the ability of treated rats to exhibit memory to a challenge with BP-CFA. We (16-17) and others (18-19) have reported that following recovery from passively induced EAE, or in the case of neonates, in the absence of any initial disease symptoms following cell transfer (16- 17), a later active challenge with BP-CFA leads to a much earlier onset of disease symptoms than is seen in control animals which never received EAE effector cells. The interpretation of these data is that early onset is the result of activation of memory cells which persist in the animal and arise from the original transfer population.

Therefore, the animals represented in Table xi were challenged with 50 g BP-CFA on day 14 after receiving the cell transfer. Control rats which had never received cells were also challenged. The results are shown in Table XII Naive animals developed disease day 10-11 after active immunization. Cell recipients on the other hand showed a memory response following challenge irrespective of treatment regimen or presence or absence of initial clinical signs, thus demonstrating that treatment did not inhibit adoptively transferred disease by killing the cells.

2 Is the effect that heparin, and possibly the other polysaccharides have on adoptively transferred EAE a function of their anticoagulant activity? To answer this question, anticoagulant free heparin was prepared by

_U

WO 88/05301 39 PCT/AU88/00017 periodate oxidation and borohydride reduction as described in MATERIALS AND METHODS and then tested for its EAE inhibiting activity. As seen in TablexIIT,all animals receiving anticoagulant free heparin developed EAE; however, there was a significant delay in onset of disease, a diminution in the clinical severity and also a decrease in the duration of the disease symptoms. These results strongly suggest that the EAE inhibiting effect of heparin is not due solely to its anticoagulating activity.

The effect of heparin on actively induced EAE was also examined and the results presented in TableXIV. When heparin treatment was begun at the time of sensitization there was a sgnificant delay in the onset of disease, however, the clinical score attained or the duration of disease did not differ from controls animals. It is interesting to note that the delay of 6 days is approximately the length of time the pumps deliver heparin, 7 days.

PCT/AU88/00017 wo 88/05301

TABLEX

Effect of heparin on adoptively transferred EAEa Treatment Rats with EAE/ Mean Day Mean Clinical Total of Onset Score Control 4/4 4.3. 0.2 3.9 ±0.1 Heparinb 3/6 5.3 0 3 c 3.5 ±0.2

C

mg/ml) Heparinb 1/5 7.0 c 2.5 c mg/ml) a. 30x10 6 Con A incubated EAE spleen cells given iv.

b. Heparin given in osmotic pumps placed subcutaneously at time of cell transfer.

c. Represent the score only for the animal(s) which developed clinical signs.

S88/0301 WO 88/05301 4 1 PCT/AU88/00017 TABLE XI Effect of sulfated polysaccharides on adoptively transferred EAE Treatment With Mean Day Mean Mean EAE/ Onset Clinical Length Total Score Disease Control 7/7 4.4 ±0.2 4.1 ±0.9 5.0 ±0.4 Heparin 0/8 mg/ml) Fucoidan 0/6 mg/ml) Chondroitin- 4-sulfate 5/5 5 3.5 ±0.2 5.0 ±0.4 mg/ml) Pentosan sulfate 5/5 6.2 ±0.3 2.4 ±0.2 3.0 ±0.4 mg/ml) a. Osmotic pumps containing sulfated polysaccharides were implanted day 3 after cell transfer.

L _1 cl B> at PCT/AIJ88/00017 WO 88/05301 TABLE XII Memory response in rats receiving sulfated polysaccharides 3 days after adoptive transfer of EAE effector cells Treatment With EAE/ Individual Group Challenged a Day of Onset Naive 4/4 4/4 Control (cells only) 10, 11, 11, 11 7, 7, 7, 8 7, 7, 7, 8, 8 7, 7, 8, 8, 8, Heparin Fucoidan 5/5 6/6 5/5 Chondroitin- 4-sulfate 7, 7, 8, 8, N.D. Pentosan sulfate N.D.b a. Rats challenged with 50 Ag BP-CFA on day 14 after initial cell transfer.

b. Not determined.

WO 88/05301 PCT/AU88/000 17 TABLE XIII Effect of anticoagulant-free heparin on passively induced EAE Treatment With EAE/ Mean Mean Mean Total Day Clinical Duration Onset Score Disease Control 7/7 5 .0 3 .4 0O.l1 4.0 j-0.2 Hepari n mg/mi Period ateoxidised 5/5 6.4 ±0.2 1.6 ±0.2 2.4 ±0.4 h e pa ri n mg/mi Li I- PCT/AU88/00017 WO 88/05301 TABLE XIV on actively Effect of heparin induced EAE Treatment #f With EAE/ Mean Mean Mean Challenged Day Clinical Duration Onset Score Disease Control 5/5 1 1 .4 4. 2 fO. 3 6.6 tO 6 H(,)ari na 5/5 17 .6 0 .8 5.0 6.0 ±Q mg/ml a Heparin pumps implanted at time of sensitization wi th 50 BP- CFA/r at L- WO 88/05301 PCT/A U88/000 17

REFERENCES

1. BADENOCH-JONES, P. and RAMSHAW, Aust.

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2. BRENAN, and PARISH, C.R.

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3. PARISH, C.R. and SNOWDEN, J.M. Cell Imrnunol., 91, 201-214 (1985).

4. RAI4SHAW, CARLSEN, WANG, and BADENOCH-JONES, P. Int,J.Cancer. 32,. 471-478 (1983).

REID, Cloning. In: Jakoby, W.B., and Pastan, I.G. (eds). Methods of Enzymology. Vol. LVIII, pp 152-164, Academic Press, New York (1979).

6. RICKLES, F.R. and EDWARDS, R.L. Blood, 62, 14-31 (1983).

7. SOKAL, R.R. and ROHLF, F.J. Biometry.

W.H.Freman and Co., San Francisco, p.770 (1969).

8. WELCH, NERY, and NICOLSON, G.L.

Invas. Metast. 3, 65-80 (1983).

9. FRANSSON, L-A. Carbohydr. Res. 62, 235-244 (1978).

LEVVY, G.A. and McALLAN, A. Biochem. J. 73, 127-133 (1959).

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Claims (6)

1. A method of anti-metastatic and/or anti-inflammatory treatment of an animal or human patient in need of such treatment, which comprises administration to the patient of an effective amount of at least one sulphated polysaccharide which blocks or inhibits endoglycosidase activity as sole anti- metastatic and/or anti-inflammatory active agent.

2. A method according to claim 1, wherein said sulphated polysaccharide is one which blocks or inhibits heparinase activity. 0

3. A method according to claim 1, wherein said sulphated polysaccharide is selected from the group consisting of heparin (including modified heparin), fucoidan, pentosan sulphate, dextran sulphate and carrageenan-lambda.

4. A method according to claim 3, wherein said sulphated polysaccharide is heparin.

5. A method according to claim 3, wherein said sulphated polysaccharide is heparin which has been modified to reduce its anti-coagulant activity. 00

6. A method according to claim 5, wherein said modified heparin is decarboxylated heparin or periodate oxidized, reduced heparin. 901010,jmsres.020,12410.res,47 48 which blocks or inhibits endoglycosidase activity as salf_ *-metas tatic and/or anti-inflamrmatory active agent for t parationof a medicament for anti- and/or n matr reatment of an animal or human patient Dated this 10th day of October, 1990. DAVIES COLLISON Patent Attorneys for 00 THE AUSTRALIAN NATIONAL UNIVERSITY aft, 901t110,msres.020,12410.res,48