AU589005B2

AU589005B2 - Virucidal composition

Info

Publication number: AU589005B2
Application number: AU72878/87A
Authority: AU
Inventors: Timothy Paul Masters
Original assignee: VETHEALTH Ltd
Current assignee: VETHEALTH Ltd
Priority date: 1986-04-16
Filing date: 1987-04-16
Publication date: 1989-09-28
Anticipated expiration: 2007-04-16
Also published as: GB8709209D0; GB2189146B; FI875510A0; GB2189146A; EP0275262A1; GB8609329D0; JPS63502990A; AU7287887A; FI875510A; WO1987006099A1

Description

'

VIRUCIDAL COMPOSITION

Field of the Invention

This invention relates to virucidal compositions and to methods of disinfecting using such compositions .

The invention is more specifically concerned with a virucidal 5 composition which includes glutaraldehyde .

' Description of the Prior Art

The use of sporicidal compositions containing glutaraldehyde and a cationic surfactant is disclosed in British Patent Specifica¬ tion number 1052537. In use , it has been discovered that 10 these compositions have a virucidal as well as sporicidal activity ,

A disadvantage of the particular compositions disclosed in Patent Specification number 1052537 is that aqueous solutions of the glutaraldehyde and surfactant need to be mixed with a buffering agent to enhance the activity of the composition.

15 Enveloped viruses which are surrounded by an inert sheath have been found to be treatable by such solutions . However , unenveloped viruses which have no inert sheath are much more difficult to treat . One such virus is the parvovirus , which has at least four strains , i . e . canine , feline , bovine and porcine

20 parvoviruses . It is an object of the invention to provide a virucidal composi¬ tion which is effective against unenveloped viruses and which can be used without the addition of a buffering agent .

Summary of the Invention

According to one aspect of the present invention there is provided a virucidal composition comprising glutaraldehyde and dimethylcocobenzylammonium chloride .

According to another aspect of the invention there is provided a method of disinfecting an object which comprises subjecting the object to treatment with a virucidal composition comprising glutaraldehyde and dimethylcocobenzylammonium chloride .

The virucidal composition may be used as an aqueous solution which does not contain a buffering agent . •

Description of the Preferred Embodiments

In one example of the invention a virucidal composition is prepared by introducing 200 litres of water into a mixing vessel equipped with a stirrer and adding thereto 30 grams of solubilised brown dye ( 18409 ) . With continuous stirring 150 kg of glutaraldehyde solution are added followed by 100 kg . of cationic surfactant Arquad B50. 10 kg. of an aromatic perfume are then added and , when the mixture has been made up to 500 litres by the addition of further water , the mixture is then stirred for a further twenty minutes before batches of the composition are run off into suitable containers .

The above composition is an aqueous solution of the glutaraldehyde and surfactant and is hereinafter referred to as Composition A.

The Arquad B50 included in Composition A comprises an aqueous 50% by weight solution of quaternary ammonium salt the principal component of which is dimethylcocobenzylammonium chloride. The glutaraldehyde solution of Composition A is itself a 50% by weight solution of glutaraldehyde in water. Thus, Composition A comprises 15% by weight glutaraldehyde and 10% by weight cationic surfactant.

Tests have been carried out to establish the virucidal activity of Composition A in accordance with the standard test procedures of the Ministry of Agriculture, Fisheries and Food. These tests have shown the effectiveness of Composition A against the following viruses at the dilutions quoted, which give a 4 log_jQ reduction in virus titre:-

Virus Dilution of Composition A

Parainf luenza 3 virus 1 750 Aujeszky's Disease Virus 1 1400

Paraviccima virus 1 1000

Transmissible gastroentiritis virus 1 900

Chlamydia psittaci 1 1000

Canine parvovirus 1 50

For use, therefore, as a spray in veterinary surgeries or as a disinfectant/detergent in premises where parvoviruses may be encountered, Composition A is used at a dilution in water of approximately 1:50 whereas, for general purposes, it can be used at a dilution of 1:500. At a dilution of 1:50, the concen- tration of glutaraldehyde is 0.3% by weight, the preferred concen¬ tration range being 0.2% to 1% by weight. At the same dilution the concentration of dimethylcocobenzylammonium chloride is 0.2%, the preferred concentration range being 0.1% to 0.6% by weight.

Similarly, at a dilution of 1:500, the concentration of glutaralde¬ hyde is 0.03% and the concentration of dimethylcocobenzylammonium chloride is 0 . 02 %

The preferred aromatic perfume for inclusion in the composition is that sold under the trade mark SOL-ODO-MASK H by Kilgern Chemicals Limited , of West Yorkshire .

Arquad B50 is produced by Akzo Chemie of Holland and chemical analysis of Arquad B50 shows that it comprises a number of structurally similar quaternary ammonium compounds and that the percentages by weight of the different carbon chain length compounds are as follows :

C CIIOO C C1122 C C1144 C C1166 C C1188 C20

3 54 22 10 10

The term dimethylcocobenzylammonium chloride as used herein is* thus to be interpreted as including a product one of the principal components of which has a 12 carbon chain.

Other cationic surfactants produced by Akzo Chemie include

Arquad S50 ( oleyltrimethylammoniu chloride ) and Arquad T50 ( tallow trimethylammonium chloride ) .

The percentages by weight of the different carbon chain length constituents ^"of Arquad S50 and Arquad T50 are as follows : -

CIO C12 C14 C16 C18 C20

S50 - 1 4 12 82 1

T50 - 1 4 31 64 1

Tests have been carried out to compare the effectiveness of Composition A with that of Compositions in which the Arquad B50 is replaced by Arquad S50 or Arquad T50. In these tests , a 5% aqueous solution of milk is exposed to a disinfectant for varying lengths of time and the cultui e so obtained is plated on to a blood/agar plate which is then incubated at 37 °c for 72 hours prior to examination.

The results which were obtained using 1% aqueous solutions of the disinfectants demonstrate the increased effectiveness of Composition A containing Arquad B50 and were as follows :

5 minutes 10 minutes 20 minutes 30 minutes

Arquad B50 No growth No growth No growth No growth

Arquad S50 Light Light Moderate Light haemol ysis haemolysis haemolysis haemolysis

Arquad T50 Light Moderate Severe Moderate haemolysis haemolysis haemolysis haemolysis

On repetition of the 10 minute exposure time tesjs to ascertairr colony formation , no colonies were formed with Arquad B50 , between 4 and 10 colonies were formed with Arquad S50 , and between 11 and 50 colonies were formed with Arquad T50 .

The 10 minute exposure tests were then repeated with two further surfactants produced b y Akzo Chemie . These were Arquad 2-C75._., which is dicocodimethylammonium chloride , and Ethoquad C-25 , which is polyoxyethylenecocomethylammonium chloride . The percentages b y weight of the different carbon chain length constituents of Arquad 2-C75 and Ethoquad C- 25 are : -

0! CIO C12 C14 C16 C18

2-C75 3 5 50 19 10 10

C-25 3 5 50 19 10 10

With Arquad B50 no colony growth occurred whereas with Arquad 2-C75 there was significant colony growth at two sites and with Ethoquad C-25 there was rapid colonial growth at one site .

These test results clearly demonstrate the surprising increased 5 effectiveness of dimethylcocobenzylammonium chloride as compared to other surfactants of similar structural formulae .

Composition A contains an aqueous solution of glutaraldehyde and surfactant. As an alternative thereto , the glutaraldehyde , surfactant , dye and perfume may be mixed together directly 10 without the addition of water to form a concentrate . This concentrate is storage-stable and can be produced particularly for export purposes .

Composition A, or the concentrate when dissolved in an equal weight of water , has a pH in the region of ' 7.5. The glutaralde- - 15 hyde and surfactant interact synergistically at this pH to obtain a virucidal activity which is significantly greater than either glutaraldehyde or the surfactant separately.

Claims

CLAIMS:

1. A virucidal composition comprising glutaraldehyde and dimethylcocobenzylammonium chloride .

2 . A virucidal composition according to Claim 1 , wherein the glutaraldehyde comprises a 50% by weight aqueous solution.

3 . A virucidal composition according to claim 1 , in the form of an aqueous solution containing 15% by weight glutaraldehyde and 10% by weight dimethylcocobenzylammonium chloride .

4. A virucidal composition according to claim 1 , in the form of an aqueous solution containing 0.2 to 1% by weight glutaraldehyde and 0.1 to 0.6% by weight dimethylcocobenzylammonium chloride .

5. A virucidal composition for use in combatting parvovirus infections comprising an aqueous solution of Composition A as hereinbefore described .

6. A method of disinfecting an object which comprises subject- ing the object to treatment with a virucidal composition comprising glutaraldehyde and dimethylcocobenzylammonium chloride .

7. A method according to Claim 6 , wherein the virucidal composition comprises an aqueous solution of Composition A as hereinbefore described .