AU5429199A - Modified green fluorescent protein - Google Patents

Modified green fluorescent protein

Info

Publication number
AU5429199A
AU5429199A AU54291/99A AU5429199A AU5429199A AU 5429199 A AU5429199 A AU 5429199A AU 54291/99 A AU54291/99 A AU 54291/99A AU 5429199 A AU5429199 A AU 5429199A AU 5429199 A AU5429199 A AU 5429199A
Authority
AU
Australia
Prior art keywords
fluorescent protein
green fluorescent
modified green
modified
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU54291/99A
Inventor
Philippe Bastiaens
Stefan Geley
Rainer Pepperkok
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cancer Research Horizons Ltd
Original Assignee
Imperial Cancer Research Technology Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9817227.3A external-priority patent/GB9817227D0/en
Priority claimed from GBGB9817229.9A external-priority patent/GB9817229D0/en
Priority claimed from GBGB9817225.7A external-priority patent/GB9817225D0/en
Application filed by Imperial Cancer Research Technology Ltd filed Critical Imperial Cancer Research Technology Ltd
Publication of AU5429199A publication Critical patent/AU5429199A/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Toxicology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Physics & Mathematics (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
AU54291/99A 1998-08-08 1999-08-06 Modified green fluorescent protein Abandoned AU5429199A (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
GB9817227 1998-08-08
GB9817225 1998-08-08
GBGB9817227.3A GB9817227D0 (en) 1998-08-08 1998-08-08 Multiple frequency fluorescence lifetime imaging
GBGB9817229.9A GB9817229D0 (en) 1998-08-08 1998-08-08 Fluorescent assay for biological systems
GBGB9817225.7A GB9817225D0 (en) 1998-08-08 1998-08-08 Fluorescent protein
GB9817229 1998-08-08
PCT/GB1999/002596 WO2000008054A1 (en) 1998-08-08 1999-08-06 Modified green fluorescent protein

Publications (1)

Publication Number Publication Date
AU5429199A true AU5429199A (en) 2000-02-28

Family

ID=27269431

Family Applications (3)

Application Number Title Priority Date Filing Date
AU54292/99A Abandoned AU5429299A (en) 1998-08-08 1999-08-06 Multiple frequency fluorescence lifetime imaging
AU54293/99A Abandoned AU5429399A (en) 1998-08-08 1999-08-06 Fluorescent assay for biological systems
AU54291/99A Abandoned AU5429199A (en) 1998-08-08 1999-08-06 Modified green fluorescent protein

Family Applications Before (2)

Application Number Title Priority Date Filing Date
AU54292/99A Abandoned AU5429299A (en) 1998-08-08 1999-08-06 Multiple frequency fluorescence lifetime imaging
AU54293/99A Abandoned AU5429399A (en) 1998-08-08 1999-08-06 Fluorescent assay for biological systems

Country Status (4)

Country Link
EP (3) EP1102976A1 (en)
JP (2) JP2002522040A (en)
AU (3) AU5429299A (en)
WO (3) WO2000008444A1 (en)

Families Citing this family (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100503701B1 (en) 1996-11-20 2005-07-26 인트로겐 테라페티스, 인코퍼레이티드 An improved method for the production and purification of adenoviral vectors
EP1190092A2 (en) 1999-04-06 2002-03-27 Yale University Fixed address analysis of sequence tags
US6403319B1 (en) 1999-08-13 2002-06-11 Yale University Analysis of sequence tags with hairpin primers
AU2001232219A1 (en) * 2000-08-14 2001-06-06 Michiyuki Matsuda Protein monitoring the activity of small gtp-binding protein
CA2419503A1 (en) * 2000-08-14 2003-02-14 Michiyuki Matsuda Protein monitoring the activity of low-molecular weight gtp-binding protein
AU2001260625A1 (en) * 2000-08-14 2002-02-25 Michiyuki Matsuda Activity monitor protein for low-molecular weight gtp-binding protein
GB0024351D0 (en) * 2000-10-04 2000-11-22 Amersham Pharm Biotech Uk Ltd Dye-labelled peptide and method
US6447995B1 (en) 2000-10-04 2002-09-10 Genvec, Inc. Utilizing intrinsic fluorescence to detect adenovirus
JP2005504261A (en) * 2001-01-12 2005-02-10 アフィボディ・アーベー Detection method
EP1304561A4 (en) * 2001-03-23 2006-01-11 Japan Science & Tech Agency Probe for visualizing phosphorylation/dephosphorylation of protein and method of detecting and quantifying phosphorylation/dephosphorylation of protein
GB0109858D0 (en) 2001-04-23 2001-06-13 Amersham Pharm Biotech Uk Ltd Fluorscent proteins
GB0208989D0 (en) * 2002-04-19 2002-05-29 Amersham Biosciences Uk Ltd Methods for measuring enzyme activity
EP1659944A4 (en) * 2003-08-19 2008-10-29 Cedars Sinai Medical Center Method for fluorescence lifetime imaging microscopy and spectroscopy
WO2006029449A1 (en) * 2004-09-14 2006-03-23 Btf Pty Ltd Chromosomal insertion of gfp into bacteria for quality control
DE112005002567T5 (en) * 2004-10-19 2007-11-22 Wallac Oy A new probe and its use in bioaffinity analyzes
WO2006055735A2 (en) * 2004-11-16 2006-05-26 Illumina, Inc Scanner having spatial light modulator
DE602005019791D1 (en) 2004-11-16 2010-04-15 Illumina Inc METHOD AND DEVICE FOR READING CODED MICROBALLS
DE102005005438A1 (en) * 2005-02-05 2006-08-24 Bayer Healthcare Ag Mutants of the fluorescent protein CGFPs, as well as their use
US7830575B2 (en) 2006-04-10 2010-11-09 Illumina, Inc. Optical scanner with improved scan time
DE102006026586A1 (en) * 2006-06-07 2007-12-13 Bayer Healthcare Ag Fluorescent proteins wfCGFP, as well as their use
US8063386B2 (en) 2007-01-30 2011-11-22 Ge Healthcare Bio-Sciences Corp. Time resolved fluorescent imaging system
DE102008033214A1 (en) * 2008-07-15 2010-01-21 Endress + Hauser Conducta Gesellschaft für Mess- und Regeltechnik mbH + Co. KG Method for optically determining a measured variable of a measuring medium
CA2748500C (en) 2009-01-29 2017-10-17 Commonwealth Scientific And Industrial Research Organisation Measuring g protein coupled receptor activation
CN102292630A (en) * 2009-02-10 2011-12-21 三井造船株式会社 Fluorescence detection device and fluorescence detection method
JP5569894B2 (en) * 2009-03-26 2014-08-13 学校法人東京農業大学 Screening method of test compounds for decreased memory ability
WO2011039953A1 (en) * 2009-09-29 2011-04-07 三井造船株式会社 Method and device for fret measurement
WO2014168734A1 (en) 2013-03-15 2014-10-16 Cedars-Sinai Medical Center Time-resolved laser-induced fluorescence spectroscopy systems and uses thereof
WO2014165814A1 (en) 2013-04-04 2014-10-09 Georgia State University Research Foundation, Inc. Rna microchip detection using nanoparticle-assisted signal amplification
FR3020874A1 (en) * 2014-05-12 2015-11-13 Univ Bourgogne METHOD FOR DETECTING FLUORESCENCE RESONANCE ENERGY TRANSFER AND STREAM CYTOMETER FOR IMPLEMENTING SAID METHOD
CN104359880B (en) * 2014-11-05 2017-03-15 合肥学院 Chemical preparation method of CdTe quantum dot fluorescent probe for detecting trace paraquat
JP6525199B2 (en) * 2014-11-26 2019-06-05 国立大学法人広島大学 Insulin detection method and insulin detection kit
WO2017049213A1 (en) 2015-09-16 2017-03-23 PetaOmics, Inc. Methods and compositions for genomic target enrichment and selective dna sequencing
US11181727B2 (en) 2016-03-10 2021-11-23 University Of Notre Dame Du Lac Super-sensitivity multiphoton frequency-domain fluorescence lifetime imaging microscopy
JP7266519B2 (en) 2016-04-01 2023-04-28 ブラック ライト サージカル， インコーポレイテッド Systems, devices and methods for time-resolved fluorescence spectroscopy
CN107271422B (en) * 2017-08-02 2019-08-23 华南师范大学 A method of it is separated based on excitation-emission spectrum while measuring the ratio between the ratio between Acceptor-Donor quantum yield and extinction coefficient
CN112129737B (en) * 2020-09-24 2022-03-15 华南师范大学 Method for simultaneously and automatically measuring FRET system correction parameter and donor/acceptor extinction coefficient ratio and application thereof
CN112444509B (en) * 2020-11-12 2022-04-19 中国科学技术大学 Biological probe for detecting activity of CLK3 kinase in living cells and application thereof

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4937457A (en) * 1989-02-10 1990-06-26 Slm Instruments, Inc. Picosecond multi-harmonic fourier fluorometer
WO1990009637A1 (en) * 1989-02-13 1990-08-23 Research Corporation Technologies, Inc. Method and means for parallel frequency acquisition in frequency domain fluorometry
US5151869A (en) * 1990-02-16 1992-09-29 The Boc Group, Inc. Frequency domain fluorometry using coherent sampling
US5196709A (en) * 1991-05-03 1993-03-23 University Of Maryland Systems Fluorometry method and apparatus using a semiconductor laser diode as a light source
US5625048A (en) * 1994-11-10 1997-04-29 The Regents Of The University Of California Modified green fluorescent proteins
GB9504446D0 (en) * 1995-03-06 1995-04-26 Medical Res Council Improvements in or relating to gene expression
EP0851874B1 (en) * 1995-09-22 1999-09-15 Novo Nordisk A/S Novel variants of green fluorescent protein, gfp
US6803188B1 (en) * 1996-01-31 2004-10-12 The Regents Of The University Of California Tandem fluorescent protein constructs
US6124128A (en) * 1996-08-16 2000-09-26 The Regents Of The University Of California Long wavelength engineered fluorescent proteins
AU5090498A (en) * 1997-01-07 1998-08-03 California Institute Of Technology Optical sensors of cell signaling

Also Published As

Publication number Publication date
JP2002542453A (en) 2002-12-10
JP2002522040A (en) 2002-07-23
EP1102976A1 (en) 2001-05-30
WO2000008444A1 (en) 2000-02-17
WO2000008443A9 (en) 2000-05-25
EP1102977A1 (en) 2001-05-30
AU5429299A (en) 2000-02-28
AU5429399A (en) 2000-02-28
WO2000008054A1 (en) 2000-02-17
EP1102791A1 (en) 2001-05-30
WO2000008443A1 (en) 2000-02-17

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Legal Events

Date Code Title Description
MK6 Application lapsed section 142(2)(f)/reg. 8.3(3) - pct applic. not entering national phase