AU4975300A - Dna melting curve analysis - Google Patents

Dna melting curve analysis

Info

Publication number
AU4975300A
AU4975300A AU49753/00A AU4975300A AU4975300A AU 4975300 A AU4975300 A AU 4975300A AU 49753/00 A AU49753/00 A AU 49753/00A AU 4975300 A AU4975300 A AU 4975300A AU 4975300 A AU4975300 A AU 4975300A
Authority
AU
Australia
Prior art keywords
melting curve
curve analysis
dna melting
dna
analysis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU49753/00A
Inventor
James W. Hazel
Mark A. Jensen
Aaron J. Owens
Michael Schaffer
George Tice Jr.
Ganesh Vaidyanathan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Qualicon Inc
Original Assignee
Qualicon Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qualicon Inc filed Critical Qualicon Inc
Publication of AU4975300A publication Critical patent/AU4975300A/en
Abandoned legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Optics & Photonics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AU49753/00A 1999-04-30 2000-04-26 Dna melting curve analysis Abandoned AU4975300A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US13190199P 1999-04-30 1999-04-30
US60131901 1999-04-30
PCT/US2000/011084 WO2000066777A2 (en) 1999-04-30 2000-04-26 Assay based on the analysis of dna melting curves

Publications (1)

Publication Number Publication Date
AU4975300A true AU4975300A (en) 2000-11-17

Family

ID=22451516

Family Applications (1)

Application Number Title Priority Date Filing Date
AU49753/00A Abandoned AU4975300A (en) 1999-04-30 2000-04-26 Dna melting curve analysis

Country Status (4)

Country Link
EP (1) EP1185697A2 (en)
JP (1) JP2002543408A (en)
AU (1) AU4975300A (en)
WO (1) WO2000066777A2 (en)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6852986B1 (en) 1999-11-12 2005-02-08 E. I. Du Pont De Nemours And Company Fluorometer with low heat-generating light source
DE10027113A1 (en) 1999-12-23 2001-09-27 Andreas Hoeft Method for determining microbial DNA / RNA, kit therefor and use of the method
ES2253551T3 (en) * 2001-07-25 2006-06-01 Posco METHOD OF DETECTION OF THE HYBRIDIZATION OF NUCLEIC ACIDS.
JP2005523701A (en) 2002-04-26 2005-08-11 ユニバーシティ・オブ・ユタ・リサーチ・ファウンデーション Characterization of single-stranded nucleic acids by secondary structure melting analysis using double-stranded nucleic acid-specific dyes
JP2008539727A (en) * 2005-05-03 2008-11-20 ハンディーラブ インコーポレイテッド Freeze-dried pellets
KR101095093B1 (en) 2007-02-02 2011-12-16 디지탈 지노믹스(주) Weighted-sum computation method based on competitive hybridization reaction
EP2166470A1 (en) 2008-09-19 2010-03-24 Corbett Research Pty Ltd Analysis of melt curves, particularly dsDNA and protein melt curves
US8606527B2 (en) 2009-02-27 2013-12-10 Bio-Rad Laboratories, Inc. SNP detection by melt curve clustering
WO2010111509A1 (en) * 2009-03-25 2010-09-30 Life Technologies Corporation Discriminatory positive/extraction control dna
CN102428459B (en) * 2009-05-15 2014-10-22 BioFire诊断公司 Systems and methods for automated melting curve analysis
EP2456884A1 (en) 2009-07-22 2012-05-30 E. I. Du Pont De Nemours And Company Sequences and their use for detection and characterization of e. coli o157:h7
US20130157265A1 (en) 2009-10-22 2013-06-20 Jesus Mingorance Cruz Composition, method and kit for detecting bacteria by means of sequencing
CN103890192A (en) 2011-09-28 2014-06-25 纳幕尔杜邦公司 Sequences and their use for detection and characterization of stec bacteria
GB2506375B (en) * 2012-09-27 2017-10-18 Epistem Ltd Data processing and analysis systems
CA2920636A1 (en) 2013-08-20 2015-02-23 E. I. Du Pont De Nemours And Company Sequences and their use for detection of salmonella enteritidis and/or salmonella typhimurium
CN106661626A (en) * 2014-07-18 2017-05-10 纳幕尔杜邦公司 Sequences and their use for detection of listeria monocytogenes
WO2018119443A1 (en) * 2016-12-23 2018-06-28 The Regents Of The University Of California Method and device for digital high resolution melt
EP3746565A4 (en) * 2018-01-30 2021-11-03 BioFire Defense, LLC Methods and systems for validation of a nucleic acid amplification assay
US20210324452A1 (en) 2018-09-06 2021-10-21 Hygiena, Llc Sequences and their use for detection and characterization of escherichia coli serotype o157:h7
CN109520564B (en) * 2018-11-05 2020-10-30 福建石子科技有限公司 Monitoring application method of intelligent analysis model
US11634782B2 (en) 2019-03-20 2023-04-25 Hygiena, Llc Quantification of microorganisms in samples and methods of determining quantification conditions thereof
WO2020205491A1 (en) 2019-04-05 2020-10-08 Hygiena, Llc Sequences and their use for detection and characterization of genus cronobacter
DE102020207587A1 (en) 2020-06-18 2021-12-23 Robert Bosch Gesellschaft mit beschränkter Haftung Method and control device for evaluating a luminescence signal in an analysis device for analyzing a sample of biological material and analysis device for analyzing a sample of biological material

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL93522A0 (en) * 1989-02-28 1990-11-29 Du Pont Apparatus and method for controlling a process
DE4234086A1 (en) * 1992-02-05 1993-08-12 Diagen Inst Molekularbio METHOD FOR DETERMINING NUCLEIC ACID SEQUENCES AMPLIFIED IN VITRO
US5766889A (en) * 1994-06-08 1998-06-16 The Perkin-Elmer Corporation Method for determining the characteristics of the concentration growth of target nucleic acid molecules in polymerase chain reaction sample
AU6534898A (en) * 1997-02-14 1998-09-08 E.I. Du Pont De Nemours And Company Detection of double-stranded dna in a homogeneous solution

Also Published As

Publication number Publication date
JP2002543408A (en) 2002-12-17
EP1185697A2 (en) 2002-03-13
WO2000066777A2 (en) 2000-11-09
WO2000066777A3 (en) 2001-08-02

Similar Documents

Publication Publication Date Title
AU4975300A (en) Dna melting curve analysis
HUP0201438A2 (en) Dna vaccine - pcv
AU3678100A (en) Biosensor
AU2634600A (en) Pipetter
AU2925600A (en) Genetic screening
AU2733200A (en) Dna mobility modifier
AU7933000A (en) Material analysis
AU141040S (en) Pipette
AU3178200A (en) Genetic analysis
AU2308900A (en) Analysis method
AU6458400A (en) Nucleic acid, polypeptides, assays, therapeutic methods and means
AU1882900A (en) Assay for homocysteine
AU2370901A (en) Methods for collecting genetic material
AU5049001A (en) Dna analysis
AU6382000A (en) Genome analysis
WO2001029756A8 (en) Autogrid analysis
GB9905644D0 (en) Dna
AU4808200A (en) Dna balls
AU3269401A (en) Simultaneous hybridization/priming for snp analysis
AU6871900A (en) Tsg-like gene
AU1870000A (en) Gene therapy-2
AU3444300A (en) Genetic analysis
AU1134601A (en) Assay method
AU2188401A (en) Assay method
AU5854000A (en) Dna chip

Legal Events

Date Code Title Description
MK6 Application lapsed section 142(2)(f)/reg. 8.3(3) - pct applic. not entering national phase