AU4608589A - A method of site-specific mutagenesis of dna and development of plasmid vectors - Google Patents

A method of site-specific mutagenesis of dna and development of plasmid vectors

Info

Publication number
AU4608589A
AU4608589A AU46085/89A AU4608589A AU4608589A AU 4608589 A AU4608589 A AU 4608589A AU 46085/89 A AU46085/89 A AU 46085/89A AU 4608589 A AU4608589 A AU 4608589A AU 4608589 A AU4608589 A AU 4608589A
Authority
AU
Australia
Prior art keywords
dna
site
development
plasmid vectors
specific mutagenesis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
AU46085/89A
Other versions
AU630387B2 (en
Inventor
Walter Arnold
Jorn Kalinowski
Friedrich Kassing
Petra Sabine Kautz
Alfred Puhler
Georg Thierbach
Andrea Winterfeldt
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Evonik Operations GmbH
Original Assignee
Degussa GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Degussa GmbH filed Critical Degussa GmbH
Publication of AU4608589A publication Critical patent/AU4608589A/en
Application granted granted Critical
Publication of AU630387B2 publication Critical patent/AU630387B2/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
AU46085/89A 1988-12-09 1989-12-08 A method of site-specific mutagenesis of dna and development of plasmid vectors Ceased AU630387B2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE3841454 1988-12-09
DE3841454A DE3841454A1 (en) 1988-12-09 1988-12-09 PROCESS FOR THE LOCAL MUTAGENESIS OF DNA AND DEVELOPMENT OF PLASMID VECTORS

Publications (2)

Publication Number Publication Date
AU4608589A true AU4608589A (en) 1990-06-14
AU630387B2 AU630387B2 (en) 1992-10-29

Family

ID=6368767

Family Applications (1)

Application Number Title Priority Date Filing Date
AU46085/89A Ceased AU630387B2 (en) 1988-12-09 1989-12-08 A method of site-specific mutagenesis of dna and development of plasmid vectors

Country Status (3)

Country Link
EP (1) EP0375889B1 (en)
AU (1) AU630387B2 (en)
DE (2) DE3841454A1 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4006637A1 (en) * 1990-03-03 1991-09-05 Degussa TRANSPOSON, TEST VECTORS CONTAINING THIS TRANSPOSON AND MUTAGENESIS PROCESS
JPH07108228B2 (en) * 1990-10-15 1995-11-22 味の素株式会社 Temperature sensitive plasmid
GB9114734D0 (en) * 1991-07-09 1991-08-28 Univ London Process for modifying proteins
EP1043398B1 (en) * 1992-08-10 2007-07-25 Kanegafuchi Kagaku Kogyo Kabushiki Kaisha Expression vector pUC NT and use thereof
EP1731613A3 (en) 1999-07-09 2009-03-11 Evonik Degussa GmbH Nucleotide sequence for the zwf gene
DE10109690A1 (en) 2000-09-02 2002-03-14 Degussa New nucleotide sequences encoding the metY gene
DE10217058A1 (en) 2002-04-17 2003-11-27 Basf Ag Process for the production of fine chemicals containing sulfur
US20070092951A1 (en) 2005-03-24 2007-04-26 Degussa Ag Alleles of the zwf gene from coryneform bacteria
EP2762571A1 (en) 2013-01-30 2014-08-06 Evonik Industries AG Microorganism and method for the production of amino acids by fermentation

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS59166087A (en) * 1983-03-09 1984-09-19 Dai Ichi Seiyaku Co Ltd Plasmid

Also Published As

Publication number Publication date
AU630387B2 (en) 1992-10-29
EP0375889A2 (en) 1990-07-04
EP0375889B1 (en) 1994-07-20
EP0375889A3 (en) 1990-09-12
DE3841454A1 (en) 1990-06-13
DE58908072D1 (en) 1994-08-25

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