AU4586902A - Use of thiazolidinediones for the treatment of hyperglycaemia - Google Patents

Description

AUSTRALIA

PATENTS ACT 1990 COMPLETE SPECIFICATION FOR A STANDARD PATENT Name of Applicant: Actual Inventors: Address for Service: Invention Title: SmithKline Beecham plc Robin Edwin BUCKINGHAM Stephen Alistair SMITH DAVIES COLLISON CAVE, Patent Attorneys, 1 Little Collins Street, Melbourne, 3000 Use ofthiazolidinediones for the treatment ofhyperglycaemia The following statement is a full description of this invention, including the best method of performing it known to Q.\OPER\PDB\DIVISIONALS\2541205 DIV.DOC 7/6/02 USE OF THIAZOLIDINEDIONES FOR THE TREATMENT OF HYPERGLYCAEMIA This application is a divisional of Australian Patent Application No 95470/98, the entire contents of which are incorporated herein by reference.

This invention relates to a novel method of treatment, in particular to a method for treatment and/or prophylaxis of a certain, specified hyperglycaemia.

European Patent Application, Publication Number 0,306,228 relates to certain thiazolidinedione derivatives disclosed as having hypoglycaemic and hypolipidaemic activity. One particular thiazolidinedione disclosed in EP 0306228 is 5-[4-[2-(N-methyl-

N-(

2 -pyridyl)amino)ethoxy]benzyl]thiazolidine-2,4.dione (hereinafter 'Compound W094/05659 discloses certain salts of Compound including the maleate salt.

Compound is an example of a class of anti-hyperglycaemic agents known as 'insulin sensitisers' (or 'insulin action enhancers'). In particular Compound is a thiazolidinedione insulin sensitiser.

European Patent Applications, Publication Numbers: 0008203, 0139421, 0032128, 0428312, 0489663, 0155845, 0257781, 0208420,0177353,0319189 0332331, 0332332, 0528734, 0508740; International Patent Application, Publication Numbers 92/18501, 93/02079, 93/22445 and United States Patent Numbers 5104888 and 5478852, also disclose certain thiazolidinedione insulin sensitisers.

Another series of compounds generally recognised as having insulin sensitiser activity are those typified by the compounds disclosed in International Patent Applications, Publication Numbers W093/21166 and W094/01420. These compounds are herein referred to as 'acyclic insulin sensitisers'. Other examples of acyclic insulin sensitisers are those disclosed in United States Patent Number 5232945 and International Patent Applications, Publication Numbers W092/03425 and W091/19702.

Examples of other insulin sensitisers are those disclosed in European Patent Application, Publication Number 0533933, Japanese Patent Application Publication Number 05271204 and United States Patent Number 5264451.

The Report of the Expert Committee of the Diagnosis and Classification of Diabetes Mellitus (Diabetes Care, vol 20(7), 1997, 1183-1197) states that Type 2 diabetes is characterised by fasting plasma glucose levels of 2 126mg/dl (where fasting is defined as no calorific intake for at least 8 hours). It is also described therein how the development of diabetes commonly occurs over a period of several years characterised by a rise in fasting serum glycaemia levels from levels generally considered to be normal plasma glucose levels of approximately 1 l0mg/dl through to the stated hyperglycaemia characteristic of frank Type 2 diabetes. The Report also refers to metabolic stages intermediate between normal glucose homeostasis and diabetes, including impaired glucose tolerance and impaired fasting glucose.

It is known from EP0306228 that Compound I is useful in the prophylaxis of hyperglycaemia and hence for the treatment of impaired glucose tolerance. International -1A- Patent Application, Publication number WO 95/07694 also discloses that thiazolidinediones can be used to treat impaired glucose tolerance to prevent or delay the onset of Type 2 diabetes mellitus. However, EP0306228 and WO 95/07694 do not mention the treatment of any specified range of glycaemias.

It is indicated that Compound provides a particularly beneficial effect on glycaemic control in the range of hyperglycaemias from elevated normal to ,126mg/dl, thereby delaying or preventing further elevation of the hypergylcaemia.

Accordingly, the invention provides a method for the treatment of hyperglycaemia, especially fasting hyperglycaemia, wherein plasma glucose levels are in the range of elevated normal to f126mg/dl which method comprises administering an effective non-toxic and pharmaceutically acceptable amount of an insulin sensitiser, to a mammal in need thereof.

Accordingly, the invention provides a method for improving glycaemic control in conditions characterised by hyperglycaemia, especially fasting hyperglycaemia, wherein the improvement is provided in hyperglycaemias wherein plasma glucose levels are in the range of from elevated normal to 1 26mg/dl, thereby delaying or preventing further elevation of the hypergylcaemia, which method comprises administering an effective non-toxic and pharmaceutically acceptable amount of an insulin sensitiser, to a mammal in need thereof.

In yet a further aspect, the invention provides a method for the prophylaxis of hyperglycaemia, especially fasting hyperglycaemia, wherein plasma glucose levels are in the range of>126mg/dl, which method comprises administering an effective non-toxic and pharmaceutically acceptable amount of an insulin sensitiser, to a mammal in need thereof.

One particular group of the conditions defined herein, in addition to being characterised by fasting hyperglycaemia wherein plasma glucose levels are in the range of from elevated normal to <126mg/dl are further characterised by hyperglycaemias wherein plasma glucose levels following an oral glucose tolerance test are in the range of <140mg/dl.

A further group of the conditions defined herein, in addition to being characterised by fasting hyperglycaemia in the range of from elevated normal to S126mg/dl are further characterised by hyperglycaemias wherein plasma glucose levels following an oral glucose tolerance test are in the range of from 140 to <200 mg/dl.

A suitable insulin sensitiser is a thiazolidinedione insulin sensitiser.

A suitable thiazolidinedione insulin sensitiser is Compound or a tautomeric form thereof, or a pharmaceutically acceptable salt thereof, or a pharmaceutically acceptable solvate thereof.

Other suitable thiazolidinedione insulin sensitisers include dihydro-6-hydroxy-2,5,7,8-tetramethyl-2H- 1 -benzopyran-2-yl)methoxy]phenyl]methyl]- 2,4-thiazolidinedione (or troglitazone), 5-[4-[(.1methylcyclohexyl)methoxy]benzyl] thiazolidine-2,4-dione (or ciglitazone), 2 -(5-ethylpyridin-2-yl)ethoxy]benzyl] thiazolidine-2,4-dione (or pioglitazone) or 5 -[(2-benzyl-2,3-dihydrobenzopyran)-5.

ylmethyl)thiazolidine-2,4-dione (or englitazone) In one particular aspect, the method comprises the administration of 2 to 12 mg of Compound especially when administered per day.

Particularly, the method comprises the administration of 2 to 4 ,4 to 8 or 8 to 12 mg of Compound per day.

Particularly, the method comprises the administration of 2 to 4mg of Compound especially when administered per day.

Particularly, the method comprises the administration of 4 to 8mg, such as greater than 4 for example 4.1, to 8 mg, of Compound especially when administered per day.

Particularly, the method comprises the administration of 8 to 12 mg of Compound especially when administered per day.

Preferably, the method comprises the administration of 2 mg of Compound especially when administered per day.

Preferably, the method comprises the administration of 4 mg of Compound especially when administered per day.

Preferably, the method comprises the administration of 8 mg of Compound especially when administered per day.

It will be understood that the insulin sensitiser, such as Compound is administered in a pharmaceutically acceptable form, including pharmaceutically acceptable derivatives such as pharmaceutically acceptable salts, esters and solvates thereof, as appropriate.

Suitable pharmaceutically acceptable salted forms of the insulin sensitisers, such as Compound include those described in the above mentioned patents and patent applications such as in EP 0306228 and W094/05659 for Compound A preferred pharmaceutically acceptable salt for Compound is a maleate.

Suitable pharmaceutically acceptable solvated forms of the insulin sensitisers, such as Compound include those described in the above mentioned patents and patent applications, such as in EP 0306228 and W094/05659 for Compound in particular hydrates.

The insulin sensitisers, such as Compound or, a pharmaceutically acceptable salt thereof, or a pharmaceutically acceptable solvate thereof, may be prepared using known methods, for example those disclosed in the above mentioned patents and patent -3applications, such as EP 0306228 and W094/05659 for Compound The disclosures of the above mentioned patents and patent applications, such as EP 0306228 and W094/05659, are incorporated herein by reference.

The thiazolidinedione insulin sensitisers, such as Compound may exist in one of several tautomeric forms, all of which are encompassed herein either as individual tautomeric forms or as mixtures thereof. Certain of the insulin sensitisers, such as Compound contains one or more chiral carbon atom, and hence can exist in two or more stereoisomeric forms: All such forms are encompassed herein whether as individual isomers or as mixtures of isomers, including racemates.

As used herein the term 'pharmaceutically acceptable' embraces both human and veterinary use: for example the term 'pharmaceutically acceptable' embraces a veterinarily acceptable compound.

As used herein the oral glucose tolerance test is that referenced in Diabetes Care vol 20(7), 1997, 1183-1197.

As used herein 'elevated normal' hyperglycaemia is to be taken as generally understood in the art, with reference for example to the Report of the Expert Committee of the Diagnosis and Classification of Diabetes Mellitus but is usually taken to mean glycaemias wherein plasma glucose levels are >11 Omg/dl.

In the method of the invention, the active medicaments are preferably administered in pharmaceutical composition form. As indicated above, such compositions can include both medicaments or one only of the medicaments.

Such compositions may be prepared by admixing an insulin sensitiser, such as Compound and especially 2 to 12 mg thereof, and a pharmaceutically acceptable carrier therefor.

Usually the compositions are adapted for oral administration. However, they may be adapted for other modes of administration, for example parenteral administration, sublingual or transdermal administration..

The compositions may be in the form of tablets, capsules, powders, granules, lozenges, suppositories, reconstitutable powders, or liquid preparations, such as oral or sterile parenteral solutions or suspensions.

In order to obtain consistency of administration it is preferred that a composition of the invention is in the form of a unit dose.

Unit dose presentation forms for oral administration may be tablets and capsules and may contain conventional excipients such as binding agents, for example syrup, acacia, gelatin, sorbitol, tragacanth, or polyvinylpyrrolidone; fillers, for example lactose, sugar, maize-starch, calcium phosphate, sorbitol or glycine; tabletting lubricants, for example magnesium stearate; disintegrants, for example starch, polyvinylpyrrolidone, -4sodium starch glycollate or microcrystalline cellulose; or pharmaceutically acceptable wetting agents such as sodium lauryl sulphate.

The compositions are preferably in a unit dosage form in an amount appropriate for the relevant daily dosage.

Suitable dosages for the insulin sensitisers include those disclosed in the above mentioned patents and patent applications.

SSuitable dosages, including unit dosages, of Compound comprise 1, 2, 3, 4, 6, 7, 8, 9, 10, 11 or 12 mg of Compound In the treatment involving compounds other than Compound the required dosages and formulations are generally as described in the above mentioned patent publications which as stated above are incorporated by reference herein: An example includes the administration of 2 0 0-800mg of Troglitazone, for example 200, 300 or 400mg.

In the treatment the medicaments may be administered from 1 to 6 times a day, but most preferably I or 2 times per day.

The solid oral compositions may be prepared by conventional methods of blending, filling or tabletting. Repeated blending operations may be used to distribute the active agent throughout those compositions employing large quantities of fillers.

Such operations are of course conventional in the art. The tablets may be coated according to methods well known in normal pharmaceutical practice, in particular with an enteric coating.

Oral liquid preparations may be in the form of, for example, emulsions, syrups, or elixirs, or may be presented as a dry product for reconstitution with water or other suitable vehicle before use. Such liquid preparations may contain conventional additives such as suspending agents, for example sorbitol, syrup, methyl cellulose, gelatin, hydroxyethylcellulose, carboxymethylcellulose, aluminium stearate gel, hydrogenated edible fats; emulsifying agents, for example lecithin, sorbitan monooleate, or acacia; non-aqueous vehicles (which may include edible oils), for example almond oil, fractionated coconut oil, oily esters such as esters of glycerine, propylene glycol, or ethyl alcohol; preservatives, for example methyl or propyl p-hydroxybenzoate or sorbic acid; and if desired conventional flavouring or colouring agents.

For parenteral administration, fluid unit dosage forms are prepared utilizing the compound and a sterile vehicle, and, depending on the concentration used, can be either suspended or dissolved in the vehicle. In preparing solutions the compound can be dissolved in water for injection and filter sterilized before filling into a suitable vial or ampoule and sealing. Advantageously, adjuvants such as a local anaesthetic, a preservative and buffering agents can be dissolved in the vehicle. To enhance the stability, the composition can be frozen after filling into the vial and the water removed under vacuum. Parenteral suspensions are prepared in substantially the same manner, except that the Compound is suspended in the vehicle instead of being dissolved, and sterilization cannot be accomplished by filtration. The compound can be sterilized by exposure to ethylene oxide before suspending in the sterile vehicle. Advantageously, a surfactant or wetting agent is included in the composition to facilitate uniform distribution of the compound.

Compositions may contain from 0.1% to 99% by weight, preferably from 10-60% by weight, of the active material, depending upon the method of administration.

Composition may, if desired, be in the form of a pack accompanied by written or printed instructions for use.

The compositions are prepared and formulated according to conventional methods, such as those disclosed in standard reference texts, for example the British and US Pharmacopoeias, Remington's Pharmaceutical Sciences (Mack Publishing Co.), Martindale The Extra Pharmacopoeia (London, The Pharmaceutical Press) and Harry's Cosmeticology (Leonard Hill Books).

Accordingly, the invention provides the use of an insulin sensitiser, such as Compound and especially 2 to 12 mg thereof, for the manufacture of a medicament for the treatment ofhyperglycaemia, especially fasting hyperglycaemia, wherein plasma glucose levels are in the range of from elevated normal to <126mg/dl.

The invention also provides the use of an insulin sensitiser, such as Compound and especially 2 to 12 mg thereof, for the manufacture of a medicament for improving glycaemic control in conditions characterised by hyperglycaemia, especially fasting hyperglycaemia, the improvement being provided wherein plasma glucose levels are in the range of from elevated normal to <126mg/dl, thereby delaying or preventing further elevation of the hypergylcaemia.

In yet a further aspect, the invention provides the use of an insulin sensitiser, such as Compound and especially 2 to 12 mg thereof, for the manufacture of a medicament for the prophylaxis of hyperglycaemia, especially fasting hyperglycaemia, wherein plasma glucose levels are >1 2 6mg/dl.

In particular, the present invention provides a pharmaceutical composition comprising an insulin sensitiser, such as Compound and especially 2 to 12 mg thereof, and a pharmaceutically acceptable carrier therefor, for use in the treatment of hyperglycaemia, especially fasting hyperglycaemia, wherein plasma glucose levels are in the range of from elevated normal to Sl26mg/dl or for the improvement of glycaemic control in conditions characterised by fasting hyperglycaemia, the improvement being provided in the range of hyperglycaemia wherein plasma glucose levels are in the range of from elevated normal to 5126mg/dl, thereby delaying or preventing further elevation of the hypergylcaemia or for the prophylaxis of hyperglycaemia, especially fasting hyperglycaemia, wherein plasma glucose levels are >126mg/dl.

No adverse toxicological effects are expected for the compositions or methods of the invention in the above mentioned dosage ranges.

Claims (12)

1. A method for the treatment of hyperglycaemia wherein plasma glucose levels are in the range of from elevated normal to <126mg/dl, which method comprises administering an effective non-toxic and pharmaceutically acceptable amount of an insulin sensitiser, to a mammal in need thereof.

2. A method according to claim 1, wherein the hyperglycaemia is fasting hyperglycaemia.

3. A method according to claim 2, wherein the hyperglycaemia is characterised by fasting plasma glucose levels in the range of from elevated normal to <126mg/di and is further characterised by hyperglycaemia following an oral glucose tolerance test wherein plasma glucose levels <140mg/.

4. A method according to claim 2, wherein the hyperglycaemia is characterised by fasting plasma glucose levels in the range in the range of from elevated normal to 5126mg/dl and is further characterised by hyperglycaemias by hyperglycaemia following an oral glucose tolerance test wherein plasma glucose levels are in the range of from 140 to <200 mg/dl.

A method according to any one of claims 1 to 4, wherein the insulin sensitiser is a thiazolidinedione insulin sensitiser.

6. A method according to any one of claims 1 to 5, wherein the insulin sensitiser is Compound

7. A method according to claim 6, wherein 2 to 12 mg of Compound is administered per day.

8. A method according to any one of claims 1 to 4, wherein the insulin sensitiser is selected from the list consisting of: 4 -[(3,4-dihydro-6.hydroxy-2,5,7,8. tetramethyl-2H. -benzopyran.2-yl)methoxy]phenyl]methyl.2,4-thiazolidinedione (or troglitazone), 5-(4-[(1-methylcyclohexyl)methoxy]benzyl] thiazolidine-2,4-dione (or ciglitazone), 5-[ 4 2 -(5-ethylpyridin-2-yl)ethoxy]benzyl] thiazolidine-2,4-dione (or pioglitazone) and 5-[(2-benzyl-2,3-dihydrobenzopyran)-5-ylmethyl)thiazolidine2,4- dione (or englitazone); or a tautomeric form thereof, or a pharmaceutically acceptable salt thereof, or a pharmaceutically acceptable solvate thereof.

9. A method according to any one of claims 1 to 8, wherein the insulin sensitiser is in the form of a compositions adapted for oral administration.

A method according to claim 9, wherein the composition is in unit dosage form.

11 The use of an insulin sensitiser for the manufacture of a medicament for the treatment of hyperglycaemia wherein plasma glucose levels are in the range of from elevated normal to <126mg/dl.

12. A pharmaceutical composition comprising an insulin sensitiser and a pharmaceutically acceptable carrier, for use in the treatment of hyperglycaemia wherein plasma glucose levels are in the range of from elevated normal to s126mg/dl.