AU3919900A - Method of differential display of prokaryotic messenger rna by rtpcr - Google Patents

Method of differential display of prokaryotic messenger rna by rtpcr

Info

Publication number
AU3919900A
AU3919900A AU39199/00A AU3919900A AU3919900A AU 3919900 A AU3919900 A AU 3919900A AU 39199/00 A AU39199/00 A AU 39199/00A AU 3919900 A AU3919900 A AU 3919900A AU 3919900 A AU3919900 A AU 3919900A
Authority
AU
Australia
Prior art keywords
rtpcr
messenger rna
differential display
prokaryotic
prokaryotic messenger
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU39199/00A
Inventor
William E. Bentley
Ryan T. Gill
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Maryland Biotechnology Institute UMBI
Original Assignee
University of Maryland Biotechnology Institute UMBI
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Maryland Biotechnology Institute UMBI filed Critical University of Maryland Biotechnology Institute UMBI
Publication of AU3919900A publication Critical patent/AU3919900A/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU39199/00A 1999-03-25 2000-03-24 Method of differential display of prokaryotic messenger rna by rtpcr Abandoned AU3919900A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US12603899P 1999-03-25 1999-03-25
US60126038 1999-03-25
PCT/US2000/007912 WO2000056936A1 (en) 1999-03-25 2000-03-24 Method of differential display of prokaryotic messenger rna by rtpcr

Publications (1)

Publication Number Publication Date
AU3919900A true AU3919900A (en) 2000-10-09

Family

ID=22422673

Family Applications (1)

Application Number Title Priority Date Filing Date
AU39199/00A Abandoned AU3919900A (en) 1999-03-25 2000-03-24 Method of differential display of prokaryotic messenger rna by rtpcr

Country Status (2)

Country Link
AU (1) AU3919900A (en)
WO (1) WO2000056936A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6387624B1 (en) 2000-04-14 2002-05-14 Incyte Pharmaceuticals, Inc. Construction of uni-directionally cloned cDNA libraries from messenger RNA for improved 3′ end DNA sequencing

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992007095A1 (en) * 1990-10-15 1992-04-30 Stratagene Arbitrarily primed polymerase chain reaction method for fingerprinting genomes
WO1993018176A1 (en) * 1992-03-11 1993-09-16 Dana-Farber Cancer Institute METHODS TO CLONE mRNA
US5580726A (en) * 1994-04-29 1996-12-03 Geron Corporation Method and Kit for enhanced differential display
RU2111254C1 (en) * 1994-07-11 1998-05-20 Институт молекулярной биологии им.В.А.Энгельгардта РАН Method of detection of differentially expressing template rnas and cloning the corresponding cdna fragments

Also Published As

Publication number Publication date
WO2000056936A1 (en) 2000-09-28

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Legal Events

Date Code Title Description
MK6 Application lapsed section 142(2)(f)/reg. 8.3(3) - pct applic. not entering national phase