AU3631293A - Method for producing high cell densities - Google Patents

Method for producing high cell densities

Info

Publication number
AU3631293A
AU3631293A AU36312/93A AU3631293A AU3631293A AU 3631293 A AU3631293 A AU 3631293A AU 36312/93 A AU36312/93 A AU 36312/93A AU 3631293 A AU3631293 A AU 3631293A AU 3631293 A AU3631293 A AU 3631293A
Authority
AU
Australia
Prior art keywords
reactor
producing high
high cell
cell densities
nutrients
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU36312/93A
Inventor
Herbert Markl
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of AU3631293A publication Critical patent/AU3631293A/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/34—Internal compartments or partitions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Sustainable Development (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
  • Inorganic Compounds Of Heavy Metals (AREA)

Abstract

The invention concerns a method of producing high cell densities in a reactor, wherein the culture of micro-organisms, or plant or animal cells, is supplied with nutrient and the influence of growth-inhibiting substances is reduced. In one embodiment of the process, two chambers are present in the reactor and are separated by at least one membrane. A highly concentrated solution of nutrients is fed into the chamber containing the culture, while a salive solution without any nutrients is fed into at least the other chamber to facilitate osmotic balancing through the membrane. In a further variant of the method, the growth-inhibiting metabolic products are bound to an adsorbent resin added directly into the reactor and thereby neutralized. After fermentation, a separation device is used to separate the adsorbent resin from the micro-organisms produced.
AU36312/93A 1992-03-17 1993-03-05 Method for producing high cell densities Abandoned AU3631293A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE4208429 1992-03-17
DE4208429A DE4208429C2 (en) 1992-03-17 1992-03-17 Process for generating high cell densities

Publications (1)

Publication Number Publication Date
AU3631293A true AU3631293A (en) 1993-10-21

Family

ID=6454222

Family Applications (1)

Application Number Title Priority Date Filing Date
AU36312/93A Abandoned AU3631293A (en) 1992-03-17 1993-03-05 Method for producing high cell densities

Country Status (6)

Country Link
EP (1) EP0632827B1 (en)
JP (1) JPH07504573A (en)
AT (1) ATE151811T1 (en)
AU (1) AU3631293A (en)
DE (2) DE4208429C2 (en)
WO (1) WO1993019161A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19945162A1 (en) * 1999-09-21 2001-04-26 Herbert Maerkl Process for culturing cells, membrane module, use of a membrane module and reaction system for culturing cells
WO2022103252A1 (en) * 2020-11-12 2022-05-19 Imu Education Sdn Bhd Culture medium for bacteria

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3418208A (en) * 1966-02-28 1968-12-24 Mobil Oil Corp Growing increased yields of micro-organisms
US3816261A (en) * 1968-04-30 1974-06-11 Johnson & Johnson Culture medium for lertospira organisms
GB8607046D0 (en) * 1986-03-21 1986-04-30 Fisons Plc Culture device
US4937196A (en) * 1989-08-18 1990-06-26 Brunswick Corporation Membrane bioreactor system
GB8921458D0 (en) * 1989-09-22 1989-11-08 Univ Strathclyde Cell culture apparatus

Also Published As

Publication number Publication date
WO1993019161A1 (en) 1993-09-30
JPH07504573A (en) 1995-05-25
ATE151811T1 (en) 1997-05-15
DE59306203D1 (en) 1997-05-22
DE4208429A1 (en) 1993-09-23
DE4208429C2 (en) 1996-02-08
EP0632827A1 (en) 1995-01-11
EP0632827B1 (en) 1997-04-16

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