AU3287400A - Cells coexpressing an amyloid precursor protein and an alpha-secretase and theiruses in test methods and diagnostics - Google Patents

Cells coexpressing an amyloid precursor protein and an alpha-secretase and theiruses in test methods and diagnostics

Info

Publication number
AU3287400A
AU3287400A AU32874/00A AU3287400A AU3287400A AU 3287400 A AU3287400 A AU 3287400A AU 32874/00 A AU32874/00 A AU 32874/00A AU 3287400 A AU3287400 A AU 3287400A AU 3287400 A AU3287400 A AU 3287400A
Authority
AU
Australia
Prior art keywords
die
oder
ein
das
alpha
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU32874/00A
Inventor
Falk Fahrenholz
Rolf Postina
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of AU3287400A publication Critical patent/AU3287400A/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6489—Metalloendopeptidases (3.4.24)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/24—Metalloendopeptidases (3.4.24)
    • C12Y304/24086—ADAM 17 endopeptidase (3.4.24.86), i.e. TNF-alpha converting enyzme
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Abstract

Die Erfindung betrifft eine rekombinante Zelle, die ein Substratprotein exprimiert, das mindestens einen Sequenzbereich von 18 Aminosäuren des humanen Amyloidvorläuferproteins (APP, amyloid precursor protein) oder eines homologen Proteins umfasst, wobei der Sequenzbereich die alpha -Sekretasespaltstelle sowie 6 Aminosäurereste aminoterminal und 12 Aminosäurereste carboxyterminal bezogen auf die alpha -Sekretasespaltstelle enthält, und a) entweder rekombinante Nukleinsäure enthält, umfassend mindestens ein Gen für ein Proteaseprotein, das entweder mindestens den/die für die proteolytische Aktivität notwendigen Sequenzbereich oder Sequenzbereiche der Disintegrin-Metalloprotease ADAM 10 aus dem Rind (Bos taurus), aus dem Menschen oder aus einem anderen Säugetier umfasst oder ein Mutein einer Säugetier Disintegrin-Metalloprotease ADAM 10 darstellt, das im wesentlichen die gleichen enzymatischen Eigenschaften besitzt, wobei das Gen oder die Gene unter der Kontrolle eines heterologen Promotors stehen, b) oder heterologe RNA codierend für ein Substratprotein und für ein Proteaseprotein. Die Erfindung betrifft weiterhin diverse Anwendungen der Zellen.
AU32874/00A 1999-03-08 2000-03-08 Cells coexpressing an amyloid precursor protein and an alpha-secretase and theiruses in test methods and diagnostics Abandoned AU3287400A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE19910108 1999-03-08
DE19910108A DE19910108C2 (en) 1999-03-08 1999-03-08 Cells that co-express an amyloid precursor protein and an alpha-secretase, a test procedure for the identification of alpha-secretase-active substances and one for the identification of further secretases, a test procedure for determining the susceptibility to Alzheimer's disease and the use of nucleic acids that code for an alpha-secretase , for gene therapy
PCT/EP2000/002018 WO2000056871A2 (en) 1999-03-08 2000-03-08 CELLS COEXPRESSING AN AMYLOID PRECURSOR PROTEIN AND AN α-SECRETASE AND THEIR USES IN TEST METHODS AND DIAGNOSTICS

Publications (1)

Publication Number Publication Date
AU3287400A true AU3287400A (en) 2000-10-09

Family

ID=7900090

Family Applications (1)

Application Number Title Priority Date Filing Date
AU32874/00A Abandoned AU3287400A (en) 1999-03-08 2000-03-08 Cells coexpressing an amyloid precursor protein and an alpha-secretase and theiruses in test methods and diagnostics

Country Status (5)

Country Link
EP (1) EP1161524B1 (en)
AT (1) ATE354647T1 (en)
AU (1) AU3287400A (en)
DE (2) DE19910108C2 (en)
WO (1) WO2000056871A2 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2318902T3 (en) 1998-09-24 2009-05-01 PHARMACIA & UPJOHN COMPANY LLC SECRETASE IN THE DISEASE OF ALZHEIMER.
US6844148B1 (en) 1998-09-24 2005-01-18 Pharmacia & Upjohn Company Alzheimer's disease secretase, APP substrates therefor, and uses therefor
US6699671B1 (en) 1998-09-24 2004-03-02 Pharmacia & Upjohn Company Alzheimer's disease secretase, APP substrates therefor, and uses therefor
GB2357767B (en) * 1998-09-24 2002-08-21 Upjohn Co Alzheimer's disease secretase app substrates therefor and uses therefor
US7115410B1 (en) 1999-02-10 2006-10-03 Elan Pharmaceuticals, Inc. β-secretase enzyme compositions and methods
US7456007B1 (en) 1998-12-31 2008-11-25 Elan Pharmaceuticals, Inc. β-secretase enzyme compositions and methods
WO2000047618A2 (en) 1999-02-10 2000-08-17 Elan Pharmaceuticals, Inc. HUMAN β-SECRETASE ENZYME, INHIBITORS AND THEIR COMPOSITIONS AND USES
US7514408B1 (en) 1999-12-02 2009-04-07 Elan Pharmaceuticals, Inc. β-secretase enzyme compositions and methods
JP2004000060A (en) * 2002-05-31 2004-01-08 Otsuka Pharmaceut Co Ltd METHOD FOR SCREENING COMPOUND AFFECTING PRODUCTION OF AMYLOID beta
GB0311228D0 (en) * 2003-05-16 2003-06-18 Celltech R&D Ltd Assay
US7674597B2 (en) 2004-08-02 2010-03-09 Elan Pharmaceuticals, Inc. Signaling intermediates in an in vitro model of Alzheimer's disease
EP2090664A1 (en) * 2008-02-15 2009-08-19 Universität Konstanz Neuroprotective peptides

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020082224A1 (en) * 1997-01-14 2002-06-27 Douglas J. Jolly Non-immunogenic prodrugs and selectable markers for use in gene therapy
AU3054899A (en) * 1998-03-31 1999-10-25 Chugai Seiyaku Kabushiki Kaisha Cell line not expressing beta-secretase activity
DE19849073A1 (en) * 1998-10-24 2000-04-27 Aventis Pharma Gmbh Transgene encoding amyloid precursor protein or its fragment, used to produce transgenic nematodes used, e.g. to screen for agents for treating Alzheimer's disease

Also Published As

Publication number Publication date
EP1161524B1 (en) 2007-02-21
DE19910108C2 (en) 2001-02-22
EP1161524A2 (en) 2001-12-12
WO2000056871A3 (en) 2001-03-29
DE19910108A1 (en) 2000-09-21
DE50014086D1 (en) 2007-04-05
WO2000056871A2 (en) 2000-09-28
ATE354647T1 (en) 2007-03-15

Similar Documents

Publication Publication Date Title
AU3287400A (en) Cells coexpressing an amyloid precursor protein and an alpha-secretase and theiruses in test methods and diagnostics
EP1361229A3 (en) Use of Tyrosine Kinase (PYK2) to produce antibodies
DE69520569D1 (en) AGENT FOR THE TREATMENT OF BLOOD COAGLING PROCESS DISORDERS
DE3855525T2 (en) Use of a protein-bound polysaccharide For the manufacture of a medicament for the treatment of AIDS.
DE69519929D1 (en) METHOD AND COMPOSITIONS FOR THE SPECIFIC COAGULATION OF TUMOR VESSELS
EP1439230A3 (en) Human transmembrane protein TMP-2 gene
EP0712862A3 (en) Selective acylation of epsilon-amino groups
ATE266723T1 (en) SOLUTION CONTAINING CHAOTROPIC AGENTS AND USE OF THIS SOLUTION IN PROCEDURES FOR ISOLATION OF DNA, RNA AND PROTEINS
DE59509908D1 (en) Pre-fillable, low-particle, sterile disposable syringe for the injection of preparations and processes for their manufacture
CA2182207A1 (en) Paramunity Inducer Based on Combinations of Poxvirus Components, Process to Prepare It and Its Use as Medicament
ATE181422T1 (en) METHOD FOR DIAGNOSIS OF PREECLAMPSIA
EP1036844A4 (en) Examination method, examination reagent and remedy for diseases caused by variation in lkb1 gene
AU5505198A (en) Methods for diagnosis of allergic bronchopulmonary aspergillosis
Ishikawa et al. Systemic hyalinosis (juvenile hyaline fibromatosis) Ultrastructure of the hyaline with particular reference to the cross-banded structure
WO2000026367A3 (en) Therapeutics and diagnostics for ocular abnormalities
WO1992000378A1 (en) Dna sequence for a serine protease and associated items
Yu et al. Damage and repair of the peripheral myelin sheath and node of Ranvier after treatment with trypsin.
EP1179588A4 (en) Human brain carboxypeptidase b
CA2278782A1 (en) Methods for the diagnosis, prognosis and treatment of glaucoma and related disorders
EP0284898A3 (en) Hinge peptides, process for their preparation and their use in manufacturing synthetic immunogenes
Sato et al. Ultrastructure of inborn errors of keratinization: VII. Porokeratosis Mibelli and disseminated superficial actinic porokeratosis
DE69002742T2 (en) Separation material for blood coagulation factors, its production and use.
DE69003960T2 (en) The use of deacetylated chitin in the manufacture of a medicament for the treatment of inflammatory diseases.
CA2319699A1 (en) Placental protein 13
DE19653498C1 (en) Cloning vector system with fusion sequence

Legal Events

Date Code Title Description
MK6 Application lapsed section 142(2)(f)/reg. 8.3(3) - pct applic. not entering national phase