AU3274793A - Enzymatic inverse polymerase chain reaction library mutagenesis - Google Patents

Enzymatic inverse polymerase chain reaction library mutagenesis

Info

Publication number
AU3274793A
AU3274793A AU32747/93A AU3274793A AU3274793A AU 3274793 A AU3274793 A AU 3274793A AU 32747/93 A AU32747/93 A AU 32747/93A AU 3274793 A AU3274793 A AU 3274793A AU 3274793 A AU3274793 A AU 3274793A
Authority
AU
Australia
Prior art keywords
chain reaction
polymerase chain
inverse polymerase
enzymatic inverse
reaction library
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU32747/93A
Inventor
Willem P.C. Stemmer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hybritech Inc
Original Assignee
Hybritech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hybritech Inc filed Critical Hybritech Inc
Publication of AU3274793A publication Critical patent/AU3274793A/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids

Landscapes

  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU32747/93A 1991-12-12 1992-12-10 Enzymatic inverse polymerase chain reaction library mutagenesis Abandoned AU3274793A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US80615491A 1991-12-12 1991-12-12
US806154 1991-12-12

Publications (1)

Publication Number Publication Date
AU3274793A true AU3274793A (en) 1993-07-19

Family

ID=25193448

Family Applications (1)

Application Number Title Priority Date Filing Date
AU32747/93A Abandoned AU3274793A (en) 1991-12-12 1992-12-10 Enzymatic inverse polymerase chain reaction library mutagenesis

Country Status (2)

Country Link
AU (1) AU3274793A (en)
WO (1) WO1993012257A1 (en)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6165793A (en) * 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
WO1996038591A1 (en) * 1995-06-02 1996-12-05 Incyte Pharmaceuticals, Inc. IMPROVED METHOD FOR OBTAINING FULL-LENGTH cDNA SEQUENCES
GB9519299D0 (en) 1995-09-21 1995-11-22 Farrar Gwyneth J Genetic strategy
US5789166A (en) * 1995-12-08 1998-08-04 Stratagene Circular site-directed mutagenesis
US8551970B2 (en) 1996-04-02 2013-10-08 Optigen Patents Limited Genetic suppression and replacement
GB9606961D0 (en) * 1996-04-02 1996-06-05 Farrar Gwyneth J Genetic strategy III
GB9727512D0 (en) * 1997-12-31 1998-02-25 Adprotech Plc Fuzzy genes and their application in molecular adjuvants
CA2317896C (en) 1998-01-09 2004-08-10 University Of Utah Research Foundation Method for in vitro amplification of circular dna
AU4609301A (en) * 1999-11-02 2001-05-14 Curagen Corporation Method of identifying a nucleic acid sequence
GB0008582D0 (en) * 2000-04-08 2000-05-31 Adprotech Plc DNA immunization vectors
US20020155439A1 (en) * 2000-12-04 2002-10-24 Ana Rodriguez Method for generating a library of mutant oligonucleotides using the linear cyclic amplification reaction
US6958213B2 (en) 2000-12-12 2005-10-25 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function
US20020086292A1 (en) 2000-12-22 2002-07-04 Shigeaki Harayama Synthesis of hybrid polynucleotide molecules using single-stranded polynucleotide molecules

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4959312A (en) * 1985-05-31 1990-09-25 The University Of Tennessee Research Corporation Full spectrum mutagenesis
JPH0376580A (en) * 1989-08-17 1991-04-02 Japan Tobacco Inc Escherichia coli manifestation vector and production of antiviral protein using the same

Also Published As

Publication number Publication date
WO1993012257A1 (en) 1993-06-24

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