AU2215695A

AU2215695A - Nitro compounds and their compositions having anti-inflammatory, analgesic and anti-thrombotic acitivities

Info

Publication number: AU2215695A
Application number: AU22156/95A
Authority: AU
Inventors: Piero Del Soldato; Francesco Sannicolo'
Original assignee: Nicox SA
Current assignee: Nicox SA
Priority date: 1994-05-10
Filing date: 1995-04-04
Publication date: 1995-11-29
Anticipated expiration: 2015-04-04
Also published as: GR3032078T3; HU9603107D0; CA2190087C; EP0759899B1; CA2190087A1; SI0759899T1; WO1995030641A1; AU702662B2; KR970702839A; KR100387359B1; ES2139199T3; EP0759899A1; ATE184589T1; DK0759899T3; HUT75961A; JPH09512798A; DE69512232T2; US5861426A; RU2145595C1; JP4043046B2

Abstract

PCT No. PCT/EP95/01233 Sec. 371 Date Mar. 6, 1997 Sec. 102(e) Date Mar. 6, 1997 PCT Filed Apr. 4, 1995 PCT Pub. No. WO95/30641 PCT Pub. Date Nov. 16, 1995New compounds and their compositions having anti-inflammatory, analgesic and anti-thrombotic activities, of the general formula: A-X1-NO2 or their salts, wherein: A is R(COXu)t, wherein t is zero or 1 and u is zero or 1; and X is O, NH or NR1C wherein R1C is C1-C10 alkyl; and R is(Ia) wherein R1 is acetoxoy, preferably n ortho-position with respect to -CO- and R2 is hydrogen; or derivatives of acetylsalylsalicyclic acid; and X1 is -YO- wherein Y is C1-C20 alkylene, C5-C7 cycloalkylene, oxy-alkyl derivatives and oxy-methyl benzyl derivatives.

Description

NITRO COMPOUNDS AND THEIR COMPOSITIONS HAVING ANTI-INFLAMMATORY, ANALGESIC AND ANTI-THROMBOTIC ACTIVITIES

The present invention relates to new products having anti-inflammatory, analgesic and anti-thrombotic activities.

In particular it relates to inhibitors of cyclo-oxygenase (COX).

It is known that the anti-inflammatory and anti-thrombotic efficacy, but most of all the tolerance, of NSAIDs (Non Steroid Anti- Inflammatory Drugs), also known as FANS, seem to be considerably affected by their cyclo-oxygenase (COX)-inhibiting activity in the inflammatory site as well as in healthy tissue. See for example FASEB Journal 1, 89, 1987; Bioch. Biophys. Acta 1083, 1, 1991. It is generally believed that the more potent a COX inhibitor is the more effective it isi.

The disadvantage of these products is that they are toxic.

Furthermore, it is also known that the COX-inhibiting properties seem to depend on some factors related to the physico-chemical and structural characteristics of the molecules themselves, such as for example the acidic function.

See for example J. Pharmacol. Exp. Therap. 196, 226, 1976;

Arch. Toxicol. 60, 261, 1987. The known cyclo-oxygenase inhibitors are generally acids which can be brought back to general structures, including:

carboxyl acids, either acetylated such as, for example, aspirin and triflusal, or nonacetylated such as, for example, salycilate, diflunisal, salsalate;

acetic acids, for example diclofenac, indomethacin, tolmetin, sulindac, etodolac, ketorolac;

propionic acids, such as, for instance, ibuprofen, naproxen, pirprofen, tiaprofenic acid, loxoprofen, indoprofen, oxaprozin, ketoprofen fenoprofen, fenbufen, flurbiprofen, carprofen, suprofen;

enolic acids, such as, for example, oxyphenbutazone, phenylbutazone, piroxicam, sudoxicam, tenoxicam, isoxicam, meloxicam.

See patents USP 3,558,690; USP 3,755,427; USP 3,641,127; FR 2,112,111; USP 4,035,376; USP 3,997,669; USP 3,784,701; USP 3,896,145; USP 3,600,437; USP 3,843,681; USP 3,904,682; USP 3,228,831; USP 4,161,538; USP 4,233,299; USP 3,591,584; DE 2,537,070; USP 3,161,654; USP 4,061,779; USP 4,556,672; USP 4,089,969.

The disadvantage of these products is that they are very effective but highly toxic. The importance of the acidic function lies in the fact that a masking of this function in COX inhibitors results in a virtually complete loss of its prostanoid-inhibiting properties. See Drugs 35, 504, 1988.

Also known are products which are highly effective in inhibiting cyclooxygenase and have a low toxicity even though they do not contain the acidic function in their molecule.

These products are known as nitric esters with nonacidic ending. See for example patents PCT WO 94/04484, which describes a particular group of compounds including the well known commercial product diclofenac,- PCT/EP 93/03193, which describes another specific group of compounds including the commercial products flurbiprofen and indoprofen.

The Applicant has unexpectedly found that other compounds having the termination group -ONO₂, when X₁ = -YO-, as defined hereinafter, have anti-inflammatory, analgesic and anti-thrombotic activities when used as medicaments with high efficacy in cyclo-oxigenase inhibition and have low toxicity.

A further object of the invention is that the known products as reported in PCT WO 94/04484 and PCT/EP 93/03193 and the new compounds found by the Applicant having X₁ = -YO- have a pharmaco-dynamic disadvantage. In fact, in the bio-chemical test evaluating the cyclo-oxygenase- inhibiting activity, experiments conducted by the applicant showed a high response variability, in the order of 10-40%.

This generally results in an erratic and unpredictable efficacy, so that the determination of a correct dosage is difficult.

In practice, higher doses must be administered to limit the above variability. The disadvantage lies in the risks of a higher incidence of side effects.

Another disadvantage is that these products are difficult from a formulation point of view because oral or parenteral preparations are more difficult to prepare than traditional preparations based on acidic FANS.

Molecule solubility is known be one of the most important properties affecting the molecule pharmacokinetic and dynamic processes.

For example, for parenteral administration, particularly by the intravenous route, drugs must be formulated in soluble form.

Similarly, by the oral route, the solubilisation process is decisive for absorption and interaction with the effector. In this respect, the choice of particular solvents and/or excipients, including surfactants, etc., is also toxicologically critical. For example, an intravenous formulation should not cause haemolysis or incompatibility with blood constituents.

However, there is much evidence which indicates that surfactants and apolar solvents may be irritant. See, for instance, J. Pharm. Science 72, 1014, 1983.

Trials conducted by the applicant using 0.1% Tween 80 and 1% dimethylsulphoxide to suspend nitroxybutylflurbiprofen showed that this solvent was irritant to the gastric mucous membrane.

However, it was unpredictably found that, using a NO-flurbiprofen derivative as described below which is part of the object of the present invention, the amounts of Tween 80 and dimethylsulphoxide required for suspension were lower, such that no irritant effects were caused, even though results were the same in terms of solubilisation.

It was unpredictably and surprisingly found after numerous investigations that it is possible to prepare anti-inflammatory products, as described below, having a high cyclo-oxygenase-inhibiting activity combined with low toxicity and pharmacokinetically satisfactory responses, and having a very limited response variability with an average variation coefficient of about one half that of known products pharmacodynamically, and easier to formulate as oral or parenteral preparations.

This was totally surprising and unexpected as the factors which affect the anti-inflammatory and anti-thrombotic efficacy of NSAIDs depend on a number of parameters. Therefore, it is not possible to forecast pharmacokinetics, for example the product fraction absorbed, the pharmacodynamic activity, the toxicity and the COX-inhibiting properties and, most of all, no assumptions may be made to predict or limit response variability.

Object of the present invention are compounds, or their compositions, of general formula:

A-X₁-NO₂

or their salts, for use as medicaments, in particular as anti-inflammatory or antithrombotic agents, wherein:

A = R(COX_u)_t, wherin t is zero or 1; u is zero or 1,

X = O, NH, NR_1C wherein R_1C is a linear or branched alkyl having 1 to 10 C atoms;

R is chosen from the following groups:

group I), wherein t = 1 and u = 1

wherein:

R₁ is an OCOR₃ group, wherein R₃ is methyl, ethyl or a linear or branched C₃-C₅ alkyl, or the residue of a heterocycle with a single ring having 5 or 6 atoms which may be aromatic, partially or totally hydrogenated, containing one or more heteroatoms independently chosen from O, N, and S;

R, is hydrogen, hydroxy, halogen, a linear or when permissible branched alkyl having 1 to 4 C atoms, a linear or when permissible branched alkoxyl having 1 to 4 C atoms, a linear or when permissible branched perfluoroalkyl having 1 to 4 C atoms, for example trifluoromethyl, nitro, amino, mono- or di-(C_1-4)alkylamino;

R₁ and R, together are a dioxymethylene group, with the provisos that when X = NH, then X₁ is ethylene and R₂ = H; R₁ cannot be OCOR₃ in position 2 when R₃ is methyl; nl being 0 or 1.

Preferably, in Ia) X is equal to O or -NH, R₁ is acetoxy, preferably in ortho-position, with respect to -CO-, X₁ is (CH₂-CH₂-O)₂, R₂ is hydrogen, most preferred are the following A-X₁-NO₂ compounds: 3-acetoxy-N-(2-nitroxyethyl)-benzamide, 4-acetoxy-N-(2-nitroxyethyl)-benzamide, 3-acetoxy-N-(5-nitroxypentyl)-benzamide; 2-acetoxy-n-(5-nitroxypentyl)benzamide, N-2-(nitroxyethyl)-2-propionoxy-benzamide, 2-acetoxy- 2-nitroxy-ethyl benzoate, 2-acetoxy-N-(cis-2-nitroxycyclohexyl)-benzamide, 2-acetoxy-4-chloro-N-(2-nitroxyethyl)-benz ami de , N - ( 2 - ni t roxye t hyl ) - 2 - ( ( 4 -thiazolyldinyl)carbonyloxy)-benzamide hydro chloride, 2-nicotinoyloxy-N-(2-nitroxyethyl)-benzamide, 2-acetoxy-5-nitroxypentylbenzoate;

preferably, in Ib) R₃ = CH₃, nl = 0;

X is equal to O, X₁ is ethylene: in this case lb) is the residue of acetylsalicylsalicylic acid;

Compounds Ic) of the class Ic₁) 5-amino salicylic acid derivatives (5-amino-2-hydroxybenzoic acid) are known as mesalamine when the valence is saturated with -COOH.

In compounds Ic₂) at least one of the -COOH is reacted to form the compounds of the invention. When both -COOH are reacted one obtains bifunctional compounds. When the compound is saturated with -COOH, is known as olsalazine.

Compounds Ic₃) are known, when the starting radical has a -COOH as sulfasalazine: 2-hydroxy-5-[(4-[(2-pyridinylamino)sulphonyljphenyl]azojbenzoic acid.

The preferred compounds of Ic) have X = O and u = 1 and X₁ is different from -YO-.

group II) wherein t = 1, u = 1

wherein:

R_II5 is H, a linear or branched C₁-C₃ alkyl when permissible R_II6 has the same meaning as R_II5, or, when R_II5 is H, it may be benzyl;

R_II1, R_II2 and R_III3 independently from one another are hydrogen, a linear or when permissible branched C₁-C₆ alkyl, or C₁-C₆ alkoxy, or Cl, F, Br;

R_II4 is R_II1 or bromine;

preferred are the compounds wherein R_II1, R_II2 and R_II4 are H and R_II3 is chlorine and R_II3 is in the ortho position rela- tive to NH;

R_II5 and R_II6 are H, X is equal to O, and X₁ is (CH₂-CH₂-O)₂; lIb) is the residue of 2-[(2-methyl-3-(trifluoromethyl)phenyl]amino]-3-pyridinecarboxylic acid] and when -COOH is present is known as flunixin.

Preferred compounds are those in which u = 1 and X = O.

group III), wherein t = 1, u = 1 and R is:

wherein:

R_2a and R_3a are H, a linear or when permissible branched, substituted or non-substituted C₁-C₁₂ alkyl, allyl, with the proviso that when one of the two groups is allyl, the other is H; preferably R_2a is H, an alkyl having from 1 to 4 C, R_3a is H;

R_1a is chosen from

III I)) has the following compounds:

X

wherein the meanings are as follows:

in the compound of formula (IV), residue of Ketoprofen:

R _III1 is H, SR_III3 wherein R_III3 contains from 1 to 4 C atoms, linear or when permissible branched;

R_III2 is H, hydroxy;

preferred are the compounds wherein R _III1 and R_III2 are H,

R_3a is H and R_2a is methyl, X = 0;

in the compounds of formula (XXI), residue of carprofen:

R_xxio is H, a linear or when permissible branched alkyl having from 1 to 6 C atoms, a C₁-C₆ alkoxycarbonyl bound to a C₁-C₆ alkyl, a C₁-C₆carboxylalkyl, a C₁-C₆ alkanoyl, optionally substituted with halogens, benzyl or halobenzyl, benzoyl or halobenzoyl;

R_xxi is H, halogen, hydroxy, CN, a C₁-C₆ alkyl optionally containing OH groups, a C₁-C₆ alkoxy, acetyl, benzyloxy,

SR_χχi2 wherein R_χχi.2 is an alkyl C₁-C₆; a perfluoroalkyl having from 1 to 3 C atoms, a C₁-C₆ carboxyalkyl optionally containing OH groups, NO₂, ammino, sulphamoyl, a dialkyl sulphamoyl with the alkyl having from 1 to 6 C atoms, or a difluoroalkylsulphonyl with the alkyl having from 1 to 3 C atoms;

R_XXil is halogen, CN, a C₁-C₆ alkyl containing one or more OH groups, a C₁-C₆ alkoxy, acetyl, acetamide, benzyloxy, SR_III3 as above defined, a perfluoroalkyl having from 1 to 3 C, hydroxy, a carboxyalkyl having from 1 to 6 C, NO₂, ammino, a mono- or di-alkylamino having from 1 to 6 C, sulphamoyl, a di-alkyl sulphamoyl having from 1 to 6 C, or a difluoroalkylsulphamoyl as above defined; or R_xxi together with R_XXi1 is an alkylene dioxy having from 1 to 6 C;

preferred are the compounds wherein R_xxio is H, the connecting bridge is in position 2, R_xxi is H, R_xxil is chlorine and is in the para position relative to nitrogen; R_3a is H, R_2a is methyl and X is O;

in the compounds of formula (XXXV), residue of tiaprofenic acid:

Ar is phenyl, a hydroxyphenyl optionally mono- or poly-substituted with halogen, an alkanoyl and an alkoxy having from 1 to 6 C, a trialalkyl having from 1 to 6 C, preferably from 1 to 3 C, cyclo-pentyl, cylo-hexyl, cyclo-heptyl, heteroaryl, preferably thienyl, a furyl optionally containing OH, pyridyl;

the preferred (XXXV) compounds are those wherein Ar is phenyl, R_3a is H, R_2a is methyl and X is O;

in the compound of formula (II), residue of suprofen, of which the one preferred has been shown, wherein R_3a is H, R_2a is methyl and X = O; its equivalents as described and obtained in USP 4,035,376, which is incorporated herein in full as a reference, may also be used;

in the compound of formula (VI),

of which the ones preferred indoprofen, when R_2a is CH₃ and indobufen when R_2a is equal to H, R_3a = -CH₃ and X = O have been shown;

its equivalents as described in and obtained in accordance with USP 3,997,669, which is incorporated herein in full as reference, may also be used;

in the compounds of formula (VIII),

of which the one preferred, etodolac, wherein R_2a = R_3a = H and X = O has been shown; its equivalents as described in and obtained in accordance with USP 3,843,681, which is incorporated herein in full as reference, may also be used; - in the compounds of formula (VII),

of which the one preferred, fenoprofen, wherein R_3a = X, R_2a = -CH₃ and X = O has been shown; its equivalents as described in and obtained in accordance with USP 3,600,437, which is incorporated herein in full as reference, may also be used;

- in the compounds of formula (III),

of which the preferred, fenbufen, wherein R_2a = R_3a = H and X = O has been shown; its equivalents as described in and obtained in accordance with patent USP 3,784,701, which is incorporated herein in full as a reference, may also be used;

- in the compounds of formula. (IX), residue of flurbiprofen wherein R_3a is H, R_2a is -CH₃ and X = O;

- in the compounds of formula (X), residue of tolmetin, wherein R_2a = R_3a = H and X = O;

its equivalents as described in and obtained in accordance with patent FR 1,574,570, which is incorporated herein in full as a reference, may also be used;

In class III D) the meaning is the following:

- IIIa) when it contains the -CH(CH₃)-COOH is known as pranoprofen: α-methyl-5H-[1]benzopyrano [2,3-b]pyridine-7-acetic acid. In the preferred compound R_2a = H, R_3a = CH₃, u = 1 and X =

O.

- The residue (XXX) when contains -CH(CH₃)-COOH is known as bermoprofen: dibenz[b, f]oxepin-2-acetic acid.

The preferred compound has u = 1, X = O, R_2a = H, R_3a = CH₃. - The residue of (XXXI) is known as CS-670: 2-[4-(2-oxo-1-cyclohexylidenemethyl)phenyl] propionic acid, when the radical is -CH(CH₃)-COOH.

The preferred compound has R_2a = H, R_3a = CH₃, u = 1, X = O. - The residue (XXXII) derives from the known pemedolac which contains the -CH₂COOH groups.

The preferred compound has R_2a = R_3a = H, u = 1 and X = O. - This residue (XXXIII) is known as pirazolac when is saturated with -CH₂COOH:

4-(4-chlorphenyl)-1-(4-fluorphenyl)3-pyrazolyl acid derivatives.

Preferred compounds have R_2a = R_3a = H, u = 1 and X = O.

- The residue (XXXVI) when saturated with -CH(CH₃)-COO-, is known as zaltoprofen.

When the residue is saturated with an hydroxy or an amino group or the salts of the acid, the compounds are known as dibenzothiepin derivatives.

The preferred products have a R_2a = H, R_3a = CH₃, u = 1, X = O.

The residue (XXXVII) is deriving from the known mofezolac:

3,4-di(p-methoxyphenyl)isoxazol-5-acetic acid when the residue is -CH₂-COOH.

Preferred compounds R_2a = R_3a = H, t = 1, X = O.

group IV) in which t = 1, u = 1 and R is

wherein:

R_Ivd and R_Ivd1 are at least one H and the other a linear or when permissible branched C₁-C₆ alkyl, preferably C₁ and C₂, or a difluoroalkyl with the alkyl having from 1 to 6 C, C₁ is preferred, or R_IVd and R_IVd1 together form a methylene group;

R_IV has the following meaning:

wherein the compounds of group IV) have the following meanings:

- in the compounds of formula (II):

R_iv-ii is a 1-6 C alkyl, a cycloalkyl having from 3 to 7 C, an alkoxymethyl having from 1 to 7 C, a trifluoroalkyl having from 1 to 3 C, vinyl, ethinyl, halogen, an alkoxy having from 1 to 6 C, a difluoroalkoxy with the alkyl having from 1 to 7 C, an alkoxymethyloxy having from 1 to 7 C, an alkylthiomethyloxy with the alkyl having from 1 to 7 C, an alkyl methylthio with the alkyl having from 1 to 7 C, cyano, difluoromethylthio, phenyl-or phenylalkyl substituted with the alkyl ha ving from 1 to 8 C;

preferably R_lv-li is -CH₃O, R_IVd is H and R_IVd1 is -CH₃, and is known as a residue of naproxen;

X = NH and X₁ is equal to -(CH₂-CH₂-O)₂; also preferred is the same compound wherein X is equal to O;

in the compounds of formula (X),

of which the residue of loxoprofen has been shown, the residues described in USP 4,161,538, which is incorporated herein in full as a reference, may be used as equivalents. Preferred are the compounds in which R_IVd is H and R_IVd1 is -CH₃, X = NH and X₁ is equal to (CH₂-CH₂-O)₂; also preferred is the same compound wherein X is equal to O;

in the compounds of formula (III):

R_iv-iil is a C₂-C₅ alkyl, even branched whenever possible, a C₂ and C₃ alkyloxy, allyloxy, phenoxy, phenylthio, a cycloalkyl having from 5 to 7 C atoms, optionally substituted in position 1 by a C₁-C₂ alkyl; preferred is the compound wherein R_iv-iii is

and R_IVd = H, R_IVd1 is -CH₃, a compound known as a residue of ibuprofen;

X = NH and X₁ is equal to (CH₂-CH₂-O)₂; also preferred is the same compound wherein X is equal to O;

group V)

In group V), the compounds have the following meanings: in the compounds of formula (II)

R_vii is H or a linear or when permissible branched alkyl having from 1 to 4 C;

R_vii-l is R_vii or a linear or when permissible branched alkoxy having from 1 to 4 C; Cl, F, Br; the position of R_vii-1 being o-, m- or p-;

preferred is the residue of the known ketorolac, wherein R_vii and R_vii-1 are H, and A = R and t = 0

in the compounds of formula (V),

of which the residue of the known tenidap has been shown, its equivalents as described and obtained in USP 4,556,672, which is incorporated herein in full as a reference, may also be used;

in these compounds of formula (V) A = R and t = 0, in the compounds of formula (VII)

of which the residue of the known tenoxicam has been shown, A is RCO and t = 1 and u = 0 or A is R and t = 0; its equivalents as described and obtained in patent DE 2,537,070, which is incorporated herein in full as a reference, may also be used;

in the compounds of formula (IX)

where A = R and t = 0, or A = RCO with t = 1 and u = 0, of which the residue of the known piroxicam has been shown, its equivalents as described and obtained in

USP 3,591,584, which is incorporated herein in full as a reference, may also be used;

in the compounds of formula (III)

where A = RCOO, t = 1 and u = 0 or 1; or t = 0 and A =

R, of which the residue of the known nabumetone has been shown, its equivalents as described and obtained in USP 4,061,779, which is incorporated herein in full as reference, may also be used;

in the compounds of formula (IV)

where A = RCOO, t = 1, u = 1 of which the residue of the known indomethacin has been shown, its equivalents as described and obtained in USP 3,161,654, which is incorporated herein in full as reference, may also be used.

in compounds of formula (X):

the residue (X) is known as meloxicam.

Preferred compounds are those in which t = 0.

The residue (XI) is known as ampiroxicam when the termination is -COOC₂H₅.

The preferred compounds have u = 1 and X = O; or t = O.

The residue (XII) when is saturated with -CH₂COO- is known as bromfenac.

The preferred compounds have u = 1, X = 0 and R_2a = R₃a = H; or t = 0.

The residue XIII) derives from the known Lornoxicam when the valence is saturated with H.

Preferred compounds have t = 0.

X₁ in the formula A-X₁-NO₂ is a bivalent connecting bridge chosen from the following:

-YO- where Y is:

a linear or when permissible branched C₁-C₂₀ alkylene, preferably having from 2 to 5 carbon atoms, excluding this connecting bridge when R is:

a radical of group I) except class lb) and Ic); a radical of group II) except II_b);

a radical of group III ) except class of compounds of IIID)

a radical of group IV);

a radical of group V), except X) and including -(CH₂)₄- for the compounds of formulae (III) and (IV);

or a cycloalkylene having from 5 to 7 carbon atoms optionally substituted; - wherein n₃ is 0 or an integer from 1 to 3

-

wherein nf' is an integer from 1 to 6, preferably from 1 to 3;

- wherein R_1f = H, -CH₃ and nf is an integer from 1 to 6, preferably from 2 to 4.

The compounds containing R of group I of type la) are described in patent WO92/01668 wherein the preparation methods are also described. This patent is incorporated herein in full as a reference. The compounds of type Ib) are prepared, for instance, using the method described in the Merck Index, XI Ed., 1989, page 16, n.95, for the residue of acetylsalicylsalicylic acid. The changes in the compounds of formula lb) may be obtained applying the processes described in patent WO 92/01668.

Compounds Ic) of the class Ic₁), in which the radical is a 5 -amino salicylic acid derivative (5-amino-2-hydroxybenzoic acid) known as mesalamine, when the starting radical contains -COOH, are prepared by reduction of m-nitrobenzoic acid with Zn dust and HCl (see H. Weil et al., Ber. 55B, 2664 (1922)); or by electrolitic reduction: Le Guyader, Peltier, Compt. Rend. 253, 2544 (1961). These publications are incorporated here by reference.

The starting radical Ic₂) when it contains -COOH is known as olsalazine: 3,3'-azobis(6-hydroxybenzoic acid); and it is prepared according to EP 36,636 or USP 4,528,367, here both incorporated by reference.

Compounds Ic₃) are prepared according to USP 2,396,145 here incorporated by reference.

Equivalent compounds to Ic₁), Ic₂) and Ic₃) contain the substituents indicated in the above references.

The products of the present invention having the general formula

A-X₁-NO₂ with the connecting bridges X₁ as above defined, with respect to the compounds of group I), may be obtained using the above methods of the known art or changing the known methods by introducing bridges X₁ when these are different from the connecting bridges described in the above patents.

The compounds wherein R is of group II) are described in patents WO94/04484 and USP 3,558,690 wherein the preparation methods are also described. These patents are incorporated herein in full as a reference.

The starting compound of IIb), when the valence is saturated with -COOH (flunixin), is obtained according to USP 3,337,570 and USP 3,689,653 here incorporated by reference. Compounds containing the substituents indicated in the above patents are equivalent to flunixin.

With respect to the compounds of group II), the connective bridges X₁ as above defined may be obtained using the above methods of the known art or changing the known methods by introducing bridges X₁ when these are different from the connecting bridges described in the above patents.

The compounds wherein R is of group III) are described and obtained by the processes explained in the following patents: patent application PCT/EP/93 03193; for the compounds of formula (IV) also see USP 3,641,127; for the com pounds of formula (XXI) also see USP 3,896,145; for the compounds of formula (IX), residue of flurbiprofen, also see USP 3,755,427; for the compounds of formula (II) also see USP 4,035,376; for the compounds of formula (VI) also see USP 3,997,669; for the compounds of formula (VIII) also see USP 3,843,681; for the compounds of formula (VII) also see USP 3,600,437; for the compounds of formula (III) also see USP 3,784,701. All these patents are incorporated herein in full as a reference.

The processes for the preparation of compounds of class III D) are the following:

IIIa) residue is obtained by preparing the acid compound, according to USP 3,931,205, the valence is saturated with -CH(CH₃)-COOH. Compounds containing the substituents indicated in the above patent are equivalent to pranoprofen.

The residue (XXX) is prepared through the compound with -CH(CH₃)-COOH (bermoprofen) according to USP 4,238,620 here incorporated by reference.

Other equivalent products are listed in the above patent.

The residue (XXXI) is prepared by starting from the corresponding acid -CH(CH₃)-COOH, according to USP 4,254,274. Equivalent compounds are listed in that patent.

The residue (XXXII) is prepared according to EP 238226 here incorporated by reference when the valence is saturated with -CH₂COOH. Equivalent products are reported in said patent as substituted 1,3,4,9 tetrahydropyrane [3,4-b] indole1-acetic acids.

The residue (XXXIII) is prepared by pirazolac (the valence is saturated with -CH₂COOH) , as indicated in EP 54,812 here incorporated by reference. Equivalent products are listed in the said patent.

The residue (XXXVI) is prepared according to the patent UK 2,035,311 here incorporated by reference, by starting from zaltoprofen having termination -CH(CH₃)-COO-. Equivalent products are listed in the said patent.

The process of preparation of the residue XXXVII) is obtained by starting from the Mofezolac and it is prepared according to EP 26928. Equivalent products are reported therein.

With respect to the compounds of group III), the connecting bridges X₁ as above defined may be obtained using the above methods of the known art or changing the known methods by introducing bridges X₁ when these are different from the connecting bridges described in the above patents.

The compounds wherein R is of group IV) are described in the English patent application 9320599.5 wherein the pre paration methods are also described. This patent is incorporated herein in full as a reference.

In group IV) the compounds may also be obtained: for the compounds of formula (II), using patent USP 3,904,682; for the compounds of formula (X), in accordance with patent USP 4,161,538; for the compunds of formula (III), in accordance with patent USP 3,228,831. These patents are fully included in the present application as a reference.

With respect to the compounds of group IV), the connecting bridges X₁ as above defined may be obtained using the above methods of the known art or changing the known methods by introducing bridges X₁ when these are different from the connecting bridges described in the above patents.

The compounds wherein R is of group V) are described in the Italian patent MI94A 000916 wherein the methods of preparation are also described. This patent is incorporated herein in full as a reference. In group V) the compounds may also be obtained: for the compounds of formula (II), using patent USP 4,089,969 which is incorporated herein in full as a reference; for the compounds of formula (V) may be obtained in accordance with patent USP 4,556,672 which is incorporated herein in full as a reference.

The residue (X) is prepared according to German patent 2,756,113. Equivalent products are listed in the said patent.

The residue (XI) is prepared according to the patent EP 147,177 here incorporated by reference, by starting from ampiroxicam having the termination -COOC₂H₅. Equivalent products are listed in the said patent.

The residue (XII) is prepared according to J. Medicinal Chem., vol. 27, No. 11, Nov. 1984, Walsh et al, Antiinflammatory Agents. 3. Synthesis and Pharmacological Evaluation of 2-Amino-3-Benzoylphenylacetic Acid and Analogues, here incorporated by reference. Equivalent products are listed in said publication.

The residue (XIII) is prepared by starting by the Lornoxicam, wherein the valence is saturated with H. It is prepared according to GBP 2,003,877. Equivalent products are described in said patent.

With respect to the compounds of group V), the connecting bridges X₁ as above defined may be obtained using the above methods of the known art or changing the known methods by introducing bridges, X₁ when these are different from the connecting bridges described in the above patents.

Generally, the connection between A and X₁ is, as we saw, generally, of the ester or amide type (NH or NR_1C, as defined in X) when R is of groups I), II), III), IV). All well known synthetic routes for forming these bonds may be used to form this connection.

In the case of esters of group I), III) and IV), the most direct synthetic route involves a reaction of acyl chlorides R-CO-Cl with halogen alcohols of the HO-Y-Cl, HO-Y-Br, HO-Y-I types, in the experimental conditions of the known art.

The reaction products of formula R-CO-O-Y-Cl (Br, I) may also be obtained for class II by reacting the sodium or potassium salts of said R-CO-OH acids with dihalogen derivatives of the general formula YCl₂, YBr₂ or YI₂.

The reaction products are converted into the final products by reacting with AgNO₃ in acetonitrile, in accordance with literature reports.

The general route for groups I), III), IV) is as follows:

R-CO-Cl + HO -Y- Br - - - - -> R-CO-O-Y-Br + AgNO₃ - - - - ->

A-X₁-NO₂ wherein X₁ = YO.

The general route for group II is as follows:

R-CO-ONa + Br₂Y - - - - -> R-CO-O-Y-Br + AgNO₃ - - - - -> A-X₁-NO₂ wherein X₁ = YO.

In the case of amides the synthetic route involves a reaction of the same acyl chlorides RCOCl with amino alcohols of the general formula NH₂-Y-OH, NHR_1C-Y-OH to give amides of the general formula:

R-CO-NH-Y-OH and R-CO-NR_1C-Y-OH

in accordance with known methods.

The reaction of said amides with halogenating agents such as, for example, PCl₅, PBr₃, SOCl₂, etc., leads to halogen derivatives of the general formula:

R-CO-NH-Y-Br(Cl) and R-CO-NR_1C-Y-Br(Cl).

These, by reacting with AgN0₃ in acetonitrile in accordance with known literature methods, lead to the final products A-X₁-NO₂.

The route may be outlined as follows:

wherein YO is X_X1.

An alternative route to form the esters is a reaction of the sodium or potassium salts of the acids with the nitric esters of halogen alcohols of the general formula:

NO₂-O-Y-Cl(Br, I)

to directly give the products of the invention.

The reaction route is as follows:

wherein YO is X₁.

Synthetic routes similar to those described above can be used for products Va and Vb of group V), wherein the dihalogen derivative Br₂Y is reached with enolates, for example, of tenoxicam or piroxicam. The reaction products are then converted, in acetonitrile, by reacting with AgNO₃ in accordance with the above reaction.

The general route shown below relates to the piroxicam of formula IX in group V).

The above indicated products in the various groups are used as anti-inflammatory, analgesic, and anti-thrombotic activities. For group I) no exclusion in the meanings of X₁ is necessary.

For groups II), III), IV) and V), the meaning of X₁ is limited as above indicated for these uses, when X₁ = -YO- for some compounds.

A further object of the invention is that it was surprisingly found that the products of the invention containing -ONO₂ groups are capable of having an effect inhibiting the inflammation induced by liposaccharide (LPS), and can, therefore, be used in septic shock.

This was surprising since it is well known that, generally, anti-inflammatories do not significantly change the nitrosynthetase actitivity induced by lipopolysaccharides in rats and, therefore, cannot be used in septic shock.

The products which may be used for this pharmaceutical use are the products of the general formula

A-X₁-NO₂

described above, wherein the bivalent connecting bridge X₁ has no limitation in this case, i.e. the known connecting bridges are not excluded as nothing was described in previous patents for this use.

It must be understood that when the compounds of the various groups contain at least one asymmetric carbon, the products can be used in racemic form or as single isomers.

It is in fact well known that in the therapeutic uses of the invention in general an isomeric form is more active than the others.

The following examples are being given as an explanation not a limitation of the present invention.

EXAMPLES

Example 1: Chemical Examples - Product Preparation

Example 1a:

Preparation of compound A-X₁-NO₂, wherein R belongs to class I, X₁ is -(CH₂-CH₂-O)₂-, herein referred to as ASA.NO-DEG, and having the general formula:

2-acetoxy-benzoate of 2-[2-(nitroxy)ethoxy]ethyl

Preparation of the intermediate of the formula:

2-acetoxy-benzoate of 2-[2-(chloro)ethoxy]ethyl

1.0 g of sodium hydride (NaH) (80% suspension in white mineral oil) was added portionwise to a solution of:

acetylsalicylic acid 5.6 g and

dimethylformamide 20 ml

kept at 0°C in a stream of nitrogen.

The mixture was stirred for one hour and then added dropwise over 5 hours to a stirred solution of

2,2'-dibromo-diethylether 10.0 g and

dimethylformamide 15 ml

at 25°C. The mixture was stirred continuously for 3 days, then dried at reduced pressure. The residue was treated with:

water 50 ml and

dichloromethane 50 ml.

The phases were separated and the aqueous phase was extracted further with dichloromethane 10 ml.

The pooled organic phases were washed with water (3 × 25 ml), dried (MgSO₄), decoloured with animal charcoal (1 g), and brought to dryness in vacuum.

The residue (11.2 g) was used crude for the next reaction. Preparation of ASA-NO-DEG:

8.6 g of silver nitrate were added to a solution of

ASA-(CH₂)₂-O-(CH₂)₂ Cl 11.2 g and

acetonitrile 25 ml

kept at ambient temperature and sheltered from light.

After stirring for two days, 2.2 g of silver nitrate were added.

After another two days in the same conditions, the insoluble salts were filtered and the filtrate was freed of the solvent at reduced pressure.

A residue of 7.0 g was obtained and chromatographed on a silica gel column (500 g of silica) eluting with a toluol/ethyl acetate 95/5 v/v mixture.

The fractions which were found to be uniform by TLC (Thin Layer Chromatography) were pooled and brought to dryness. They yielded 3.0 g of ASA-NO-DEG.

A ¹H NMR analysis (CDCl₃) (80MHz) provided the following data:

2.28(3H,S); 3.7(4H,m); 4 .5 (2H, t); 4.52(2H,t); 7.3 (3H,m); 7.98 (1H,dd).

The IR analysis (nujol) provided the following results.

e 1287 cm^-1.

Mass spectrometry gave a molecular weight value of 313. Example 1b:

Preparation of compound A-X₁-NO₂, wherein R belongs to class

II), X₁ is - (CH₂-CH₂-O)₂-, herein referred to as DICLOFENAC¬

NO-DEG, and having formula:

2-{N-[2,6-(dichloro)phenyl]amino}phenylacetate of 2-[2-(nitroxy)ethoxy]ethyl

Preparation of the intermediate having formula

2-{N-[2,6-(dichloro)phenyl]amino}phenylacetate of 2-[2-(broo)ethoxy]ethyl

A solution of

DICLOFENAC sodium salt 13.3 g and

dimethylformamide 25 ml

was added to a solution of

2.2'-dibromo-diethylether 12.3 g and

dimethylformamide 15 ml

kept at ambient temperature in a stream of nitrogen.

The mixture was allowed to react for two days, and the solvent was then removed at reduced pressure. The residue was treated with ethyl acetate (50 ml), washed with a 5% solution of potassium carbonate (2 × 10 ml), then with water (20 ml), dried over anhydrous sodium sulphate. The solvent was removed at reduced pressure.

The residue weight was 16 g and was used for the next reaction with no purification.

Preparation of DICLOFENAC-NO-PEG:

Silver nitrate 8 g in

acetonitrile 16 ml

were added to a solution of

DICLOFENAC -(CH₂)₂-O-(CH₂)₂-Br 16 g and

acetonitrile 30 ml

kept at room temperature and sheltered from light.

The mixture was stirred at ambient temperature for 3 days. Silver nitrate 3 g after 1 day

silver nitrate 3 g after 2 days

were then added.

The mixture was stirred for another 2 days. The insoluble salts were then filtered and the solvent removed from the filtrate at reduced pressure. The residue was treated with ethyl acetate (50 ml), the insoluble salts were then filtered and discarded. The solvent was removed from the filtrate at reduced pressure. A residue of 16.2 g was obtained and chromatographed on a silica gel column (700 g of silica) eluting first with toluol, then with a toluol/ethyl acetate 99/1 v/v mixture, finally with a toluol/ethyl acetate 98/2 v/v mixture.

The fractions found to be uniform by TLC analysis (thin layer chromatography) were pooled and brought to dryness to yield 4.38 g of DICLOFENAC-NO-DEG.

A Η-NMR analysis (CDCl₃ ) (300 MHz) provided the following data : 3 . 69 (4H, t ); 3.87 (2H,S); 4.3 (2H,m); 4.52 (2H,t); 6.55 (1H,d); 6.88 (1H, wide s exchanged for D₂O, NH); 6.97 (2H,t); 7.11 (2H,d); 7.23 (2H,d); 7.35 (2H, d).

Mass spectrometry yielded a molecular weight value of 588. Example 1c:

Preparation of compound A-X₁-NO₂, wherein R belongs to class III) and represents the residue of the compound of formula

IV, X₁ is -C₆H₅CH₂-, herein referred to as KETOPROFEN-NO-DEG, and having formula:

2-(3-benzoyl)phenylpropionate of 3-(nitroxynethyl)phenyl

Preparation of intermediate 3 -nitroxymethyl-phenol having formula:

The reagents below are used in the amounts indicated and reacted as described below:

3 -hydroxy-benzylalcohol 10 g

48% HBr by weight 50 ml

CH₂Cl₂ 30 ml

AgNO₃ 13.7 g CH₃CN 70 ml

3-Hydroxy-benzylalcohol in CH₂Cl₂ was reacted with HBr at ambient temperature for 4 hours.

CH₂Cl₂ was then evaporated at reduced pressure at 30°C after washing with an aqueous 5% NaHCO₃ solution and drying over anhydrous Na₂SO₄.

The oily residue was dissolved in CH₃CN (50 ml) and a solution of AgNO₃ in the remaining amount of CH₃CN was added dropwise. The flask was sheltered from light.

After 8 hours the AgBr precipitate was filtered and the organic phase was evaporated at reduced pressure.

The oily residue so obtained was dissolved in toluene (45 ml) and the solution was filtered on a silica gel column (400 g). The eluate was brought to dryness at reduced pressure at 30°C to give 20 g of 3-nitroxymethylphenol.

Preparation of intermediate KETOPROFEN -COCl:

a chloride of 2-(3-benzoyl)phenyl propionic acid

KETOPROFEN 20 g

thionyl chloride 50 ml

were reacted and the solution was refluxed for 45 minutes. Thionyl chloride was evaporated off at reduced pressure. An oily yellow residue weighing 21 g was obtained and used with no further purification. Preparation of KETOPROFEN-Ar-NO₂

The reagents below were used in the following amounts

KETOPROFEN -COCl 5.45 g

3-nitroxymethylphenol 3.9 g

K₂CO₃ 5.5 g

AcOEt 50 ml

, K₂CO₃ and AcOEt were added together; ketoprofen chloride was then added under nitrogen at t = 0 in 30 minutes.

The whole was allowed to react for 5 hours at ambient temperature, then diluted with H₂O (50 ml). The organic phase was washed with 5% NaOH (2 × 10 ml) and evaporated off at reduced pressure. The resulting oily residue was chromatographed on silica using a toluol/EtOAc 9.5/0.5 v/v mi xture as an eluant. The evaporation of the eluate gave KETOPROFEN-Ar-NO₂ with a yield of 85%.

A Η-NMR analysis (CDCl₃) (300 MHz) provided the following data: 1.63 (3H,d); 4.00 (1H Q); 5.37 (2H.S); 7.01-7.89 (m,13H) .

Mass spectrometry yielded a molecular weight value of 405. Example Id:

Preparation of compound A - X₁ - NO₂, herein referred to as IBUPROFEN-NO-DEG, wherein R belongs to group IV; X, is

- (CH₂-CH₂-O)₂-, A = RCOO, R residue of IBUPROFEN, having formula:

- (CH₃)₂CHCH₂C₅H₄-CH(CH₃) - The same procedure of example la was followed, using the above R, residue of IBUPROFEN, instead of residue R of group I as shown in example 1a.

Example 1e:

Preparation of compound A-X₁-NO₂, herein referred to as FLURBIPROFEN-NO-DEG, wherein R belongs to group III; X1 is

- (CH₂-CH₂-O)₂-, A = RCOO, R_3a = H, R_2a = CH₃ , R having formula:

The same procedure of example la was followed, using the above R, residue of FLURBIPROFEN, instead of residue R of group I as shown in example la.

Example If:

Preparation of compound A-X₁-NO₂, KETOROLAC-NO-DEG, wherein R belongs to group V; X₁ is - (CH₂-CH₂-O)₂-; A = R, R of formula

II, having formula

The same procedure of example la was followed, using the above R, residue of KETOROLAC, instead of residue R of group

I as shown in example la.

Example 1g:

Preparation of compound A-X₁-NO₂, TIAPROFENIC ACID NO DEG, wherein R belongs to group III; X₁ is -(CH₂-CH₂-O)₂-,

A= RCOO, R is the residue of formula XXXV, wherein R is:

The same procedure of example la was followed, using the above R, residue of TIAPROFENIC ACID, instead of residue R of group I as shown in example la.

Example 1h:

Preparation of compound A - X₁ - NO₂, NAPROXEN NO-DEG, wherein R belongs to group IV; X₁ is - ( CH₂-CH₂-O) ₂ - , A = RCOO , R is the residue of formula II of NAPROXEN, having the general formula

The same procedure of example la was followed, using the above R, residue of NAPROXEN, instead of residue R of group I as shown in example 1a.

EXAMPLE 2: Pharmacological Examples

The products used above were pharmacologically characterised.

Example 2a: ASA-NO-DEG as prepared in example 1a; Example 2b: DICLOFENAC-NO-DEG as prepared in example lb;

Example 2c: KETOPROFEN-NO-DEG as prepared in example lc;

Example 2d: IBUPROFEN-NO-DEG as prepared in example Id;

Example 2e: FLURBIPROFEN-NO-DEG as prepared in example le;

Example 2f: KETOROLAC NO-DEG as prepared in example If;

Example 2g: TIAPROFENIC ACID NO-DEG as prepared in example

1g;

Example 2h: NAPROXEN NO-DEG as prepared in example lh.

Toxicity

Acute toxicity was evaluated by orally administering a single dose of 1, 3, 10, 30, 100 mg/Kg of product groups of 10 mice.

The death rate and the occurence of toxic symptoms were reported over an observation period of 14 days. Even after administration of a 100 mg/Kg dose the animals showed no sign of apparent toxicity.

Anti-inflammatory activity

Anti-inflammatory activity was determined by the carrageenin-oedema method as described by Winter et al. (Proc. Soc. Exp. Biol. Med. Ill, 544, 1962) in rats.

Analgesic activity

Analgesic activity was determined in Swiss mice as described by Hendershot et al. (J. Pharmacol. Exp. Therap. 125, 237, 1959).

Tolerance

Gastric tolerance was measured by oral administration to rats assessing the severity of the gastropathy induced in accordance with the criteria described by Wallace et al. (Am. J. Physiol. 259, G642, 1990).

Platelet anti-aggregating activity

Platelet anti-aggregating activity was evaluated in vitro on human platelets stimulated by thrombin in accordance with the method described by Bertele et al . (Science 220, 517. 1983).

Vasodilative activity

Vasodilative activity was determined in isolated rat aorta measuring the inhibition of the contraction induced by epinephrine in the tissue prepared in accordance with the method described by Reynolds et al. (J. Pharmacol. Exp. Therap. 252, 915, 1990).

COX Inhibition

The activity inhibiting cyclo-oxygenase was determined in isolated cells. Endothelial cells of bovine aorta were used as a source of COX-1 and macrophage line J774.2 as a source of COX-2. The same conditions described byMitchell et al. (Proc. Nat. Acad. Sci. 90, 11693, 1993) for growth and the viability test were used.

In brief, the cells were incubated for 30 minutes with scalar concentrations of the test product and the substrate (arachidonic acid) was then added and incubated for another 15 minutes. Enzyme activity was determined radioimmunologically by measuring the formation of 6-keto-PGF 1 alpha. In the case of cell lines J.774.2, the cells were incubated for 12 hours with endotoxin to promote COX-2 formation.

Nitrosynthetase inhibition by LSP

The nitrosynthetase inhibition activity induced by lipopolysaccharide (LPS) was determined in rat neutrophils and stomach after administration of one of the test compounds and compared with that obtained after treatment of the suspension vehicle only.

In brief, Wistar rats fasting for 24 hours before treatment were orally administered the test product (10 mg/Kg) and intravenously (caudal vein) administered LPS (5 mg/Kg).

Four hours later the animals were sacrificed and the blood - for neutrophils isolation - and the stomach taken.

Enzyme activity was determined in accordance with the method described by Assreuy et al. (Br. J. Pharmacol. 108, 833, 1993). Results:

The results obtained are described below.

As it may be observed from the data shown in tables 1 to 4, the pharmacodynamic activities (I and II in Table 1; Table 2) and the tolerance (Table 1 column III) of the nitroderivatives show a better balance as compared to natural products.

Table 4 also shows that, similarly to diclofenac nitroxybutylester, the diclofenac nitroderivative which is an object of this patent is capable of directly inhibiting cyclo-oxygenase COX-1 and COX-2, but with a significantly lower variability.

TABLE 1 (Pharmacology col. I and II; Toxicology col. Ill)

Study of the anti-inflammatory (I) and analgesic (II) properties (pharmacodynamics) and gastrointestinal tolerance (III) (toxicity) of the test compounds after oral administration of doses ranging from 3 to 30 mg/Kg in carboxymethylcellulose suspensions and constructing doseresponse curves. The results shown are the potency ratio as compared to the reference standard.

Activities are expressed as the potency ratio compared to the natural product used as a unit standard. The nitroderivative is that of the shown examples, the natural

Claims

1. Compounds, or their compositions, of the general formula:

A - X₁ - NO₂

or their salts, wherein:

A = R(COX_u)_t, wherein t is zero or 1; u is zero or 1, X = O, NH, NR_1C wherein R_1C is a linear or branched alkyl having 1 to 10 C atoms;

R is chosen from the following groups:

- ggroup I), wherein t = 1 and u = 1

wherein:

R₁ is an OCOR₃ group, wherein R₃ is methyl, ethyl or a linear or branched C₃-C₅ alkyl, or the residue of a heterocycle with a single ring having 5 or 6 atoms which may be aromatic, partially or totally hydrogenated, containing one or more heteroatoms independently chosen from O, N, and S;

R₂ is hydrogen, hydroxy, halogen, a linear or when permissible branched alkyl having 1 to 4 C atoms, a linear or when permissible branched alkoxyl having 1 to 4 C atoms, a linear or when permissible branched perfluoroalkyl having 1 to 4 C atoms, for example trifluoromethyl, nitro, amino, mono- or di-(C_1-4)alkylamino;

R₁ and R₂ together are a dioxymethylene group, with the proviso that when X = NH, X₁ is ethylene and R₂ = H; R₁ cannot be OCOR₃ in position 2 when R₃ is methyl; nl being 0 or 1;

- group II) wherein t = 1 and u = 1

wherein:

R_II5 is H, a linear or when permissible branched C₁-C₃ alkyl, R_II6 has the same meaning as R_II5, or, when R_II5 is

H, it may be benzyl;

R_II1, R_II2 and R_III3, independently from one anotner, are hydrogen, a linear or when permissible branched C₁-C₆ alkyl or C₁-C₆ alkoxy, or Cl, F, Br;

R_II4 is R_II1 or bromine;

- ggroup III), wherein t = 1, u = 1 and R is:

wherein:

R_2a and R_3a are H, a linear or when permissible branched, substituted or nonsubstituted C₁-C₁₂ alkyl, allyl, with the proviso that when one of the two groups is allyl, the other is H; preferably R_2a is H, an alkyl having from 1 to 4 C, R_3a is H;

R_1a is chosen from

wherein the meanings are as follows:

- in the compound of formula (IV), residue of Ketoprofen:

R_III1 is H or SR_III3 wherein R_III3 contains from 1 to 4 C atoms, linear or when permissible branched;

R_III2 is H, hydroxy;

preferred are the compounds wherein R_III1 and R_III2 are H,

R_3a is H and R_2a is methyl, X = 0;

- in the compounds of formula (XXI), residue of carprofen:

R_XXio is H, a linear or when permissible branched alkyl having from 1 to 6 C atoms, a C₁-C₆ alkoxycarbonyl bound to a C₁-C₆ alkyl, a C₁-C₆carboxylalkyl, a C₁-C₆ alkanoyl, optionally substituted with halogens, benzyl or halobenzyl, benzoyl or halobenzoyl;

Rxxi is H, halogen, hydroxy, CN, a C₁-C₆ alkyl optionally containing OH groups, a C₁-C₆ alkoxy, acetyl, benzyloxy,

SR_XXi2 wherein R_XXi2 is an alkyl C₁-C₆; a perfluoroalkyl having from 1 to 3 C atoms, a C₁-C₆ carboxyalkyl optionally containing OH groups, NO₂, ammino, sulphamoyl, a dialkyl sulphamoyl with the alkyl having from 1 to 6 C atoms, or a difluoroalkylsulphonyl with the alkyl having from 1 to 3 C atoms;

R_XXi1 is halogen, CN, a C₁-C₆ alkyl containing one or more OH groups, a C₁-C₆ alkoxy, acetyl, acetamide, benzyloxy, SR_III3 as above defined, a perfluoroalkyl having from 1 to 3 C, hydroxy, a carboxyalkyl having from 1 to 6 C, NO₂, ammino, a mono- or di-alkylamino having from 1 to 6 C, sulphamoyl, a di-alkyl sulphamoyl having from 1 to 6 C, or a difluoroalkylsulphamoyl as above defined; or R_XXi together with R_XXil is an alkylene dioxy having from 1 to 6 C;

preferred are the compounds wherein R_XXio is H, the connecting bridge is in position 2, R_XXi is H, R_XXi1 is chlorine and is in the para position relative to nitrogen; R_3a is H, R_2a is methyl and X is O;

- in the compounds of formula (XXXV), residue of tiaprofenic acid:

Ar is phenyl, a hydroxyphenyl optionally mono- or poly- substituted with halogen, an alkanoyl and an alkoxy having from 1 to 6 C, a trialalkyl having from 1 to 6 C, preferably from 1 to 3 C, cyclo-pentyl, cylohexyl, cyclo-heptyl, heteroaryl, preferably thienyl, a furyl optionally containing OH, pyridyl;

the preferred (XXXV) compounds are those wherein Ar is phenyl, R_3a is H, R_2a is methyl and X is O;

- in the compound of formula (II), residue of suprofen, wherein R_3a is H, R_2a is methyl and X = O, or its equivalents;

- in the compound of formula (VI), residue of indoprofen, wherein R_2a is CH₃, and residue of indobufen

wherein R_2a is H, R_3a = -CH₃, and X = 0 and its equivalents;

- in the compounds of formula (VIII), residue of etodolac, wherein R_2a = R_3a = H and X = 0 and its equivalents; - in the compounds of formula (VII), residue of fenoprofen, wherein R_3a = X, R_2a = -CH₃ and X = 0 and its equivalents;

- in the compounds of formula (III), residue of fenbufen, wherein R_2a R_3a = H and X = 0 and its equivalents;

- in the compounds of formula (IX), residue of flurbiprofen, wherein R_3a is H, R_2a is -CH₃ and X = 0;

- i in the compounds of formula (X), residue of tolmetin, wherein R_2a = R_3a = H and X = 0 or its equivalents;

- compound IlIa), when it contains the -CH(CH₃)-COOH, is pranoprofen residue: α-methyl-5H- [1] benzopyrano [2,3b]- pyridine-7-acetic acid;

the preferred compound has R_2a = H, R_3a = -CH₃, u = 1 and X = O;

- compound (XXX), when it contains -CH (CH₃ ) -COOH is bermoprofen residue: dibenz[b,f]oxepin-2-acetic acid; the preferred compound has u = 1, X = O, R_2a = H, R_3a = CH₃;

- compound (XXXI) is CS-670 residue: 2-[4-(2-oxo-1- cyclohexylidenemethyl)phenyl] propionic acid, when the radical is -CH(CH₃)-COOH; the preferred compound has R_2a = H, R_3a = CH₃, u = 1, X = 0;

- compound (XXXII) derives from the pemedolac which contains the -CH₂COOH groups; the preferred compound has R_2a = R_3a = H, u = 1 and X = O;

- compound (XXXIII) is pirazolac residue when is saturated with -CH₂COOH: 4-(4-chlorphenyl)-1-(4-fluorphenyl)3-pyrazolyl acid derivatives; the preferred compounds have R_2a = R_3a = H, u = 1 and X = O;

- compound (XXXVI) when saturated with -CH(CH₃)-COO-, is zaltoprofen residue, when saturated with an hydroxy or an amino group or the salts of the acid is one of the dibenzothiepin derivatives; the preferred products have a R_2a = H, R_3a = CH₃, u = 1, X = 0;

- compound (XXXVII) is deriving from the mofezolac: 3,4- di(p-methoxyphenyl)isoxazol-5-acetic acid, when the residue is -CH₂-C00H; the preferred compounds are R_2a =

R_3a = H, t = 1, X = O;

group IV) in which t = 1, u = 1 and R is

wherein:

R_Ivd and R_Ivd1 are at least one H and the other a linear or when permissible branched C₁-C₆ alkyl, preferably C₁ and C₂, or a difluoroalkyl with the alkyl having from 1 to 6 C, C₁ is preferred, or R_Ivd and R_1vdx together form a methylene group;

R_IV has the following meaning:

wherein the compounds of group IV) have the following meanings:

- in the compounds of formula (II):

R_iv-ii is a 1-6 C alkyl, a cycloalkyl having from 3 to 7 C, an alkoxymethyl having from 1 to 7 C, a trifluoroalkyl having from 1 to 3 C, vinyl, ethinyl, halogen, an alkoxy having from 1 to 6 C, a difluoroalkoxy with the alkyl having from 1 to 7 C, an alkoxymethyloxy having from 1 to 7 C, an alkylthiomethyloxy with the alkyl having from 1 to 7 C, an alkyl methylthio with the alkyl having from 1 to 7 C, cyano, difluoromethylthio, phenyl-or phenylalkyl substituted with the alkyl having from 1 to 8 C; preferably R_iv-li is -CH₃O, R_IVd is H and R_Ivd1 is -CH₃, which is a residue of naproxen,X = NH or O and X₁ is equal to -(CH₂-CH₂-O)₂;

- in the compounds of formula (X), the residue of loxoprofen or its equivalents; preferred are the compounds in which R_Ivd is H and R_1vd1 is -CH₃, X = NH or O and X₁ is equal to (CH₂-CH₂-O)₂; - in the compounds of formula (III): R_iv-iii is a C₂-C₅ alkyl, even branched whenever possible, a C₂ and C₃ alkyloxy, allyloxy, phenoxy, phenylthio, a cycloalkyl having from 5 to 7 C atoms, optionally substituted in position 1 by a C₁-C₂ alkyl;

preferred is the compound wherein R_iv-iii is

and R_Ivd = H, R_Ivd1 is -CH₃, which is a residue of ibuprofen;

X = NH or O and X₁ is equal to (CH₂-CH₂-O)₂;

- group V)

( in group V), the compounds have the following meanings: in the compounds of formula (II), R_vii is H or a linear or when permissible branched alkyl having from 1 to 4

C; R_vii-l is R_vii or a linear or when permissible branched alkoxy having from 1 to 4 C; Cl , F, Br; the position of

R_vii-1 being o-, m- or p-; preferred is the residue of

Ketorolac, wherein R_vii and R_vii-1 are H, and A = R and t =

0

in the compounds of formula (V), wherein A = R and t =

0, the residue of tenidap or its equivalents;

in the compounds of formula (VII), wherein A is RCO and t = 1 and u = 0 or A is R and t = 0; the residue of tenoxicam or its equivalents;

in the compounds of formula (IX) where A = R and t = 0, or A = RCO with t = 1 and u = 0, the residue of piroxicam or its equivalents;

in the compounds of formula (III) where A = RCOO, t = 1 and u = 0 or 1; or t = 0 and A = R the residue of nabumetone or its equivalents;

in the compounds of formula (IV) where A = RCOO, t = 1, u = 1 or the residue of indomethacin or its equivalents;

In compounds of formula (X) the residue of meloxicam, the preferred compounds are those in which t = 0;

- the residue (XI) ampiroxicam when the termination is -COOC₂H₅; the preferred compounds have u = 1 and X =

0; or t = 0;

- the residue (XII) when saturated with -CH₂COO- is bromfenac,- the preferred compounds have u = 1, X = 0 and R_2a = R₃a = H; or t = 0;

- the residue XIII) derives from Lornoxicam when the valence is saturated with H, the preferred compounds have t = 0;

X₁ in the formula A-X₁-NO₂ is a bivalent connecting bridge chosen from the following:

- -YO- where Y is:

- a linear or when permissible branched C₁-C₂₀ alkylene, preferably having from 2 to 5 carbon atoms, excluding this connecting bridge when R is:

a radical of group I) except classes lb and Ic; . a radical of group II) excpet II_b)_;

. a radical of group III) except class compounds of IIID);

. a radical of group IV);

. a radical of group V), except X) and inclu ding -(CH₂)₄- for the compounds of formulae (III) and (IV);

- or a cycloalkylene having from 5 to 7 carbon atoms optionally substituted; - wherein n₃ is 0 or an integer from 1 to 3

-

-

wherein nf' is an integer from 1 to 6, preferably from 2 to 4;

-

wherein R_1f = H, -CH₃ and nf is an integer from 1 to

6, preferably from 2 to 4.

The compounds or their compositions according to claim 1, wherein, in group I): in the compounds of formula la):

X is O, R1 is acetoxy, preferably in ortho-position with respect to -CO-, X₁ is (CH--CH₂-0) ,, R₂ is hydrogen; in Ib): R₃ = CH₃, nl = 0, X is equal to 0, X_x is ethylene; in this case lb) is the residue of acetylsalicylsalicylic acid;

in group II: where R_II1, R_II2 and R_II4 are H, R_II3 is chlorine and R_II3 is in the ortho position relative to NH; R_II5 and R_II6 are H; X is equal to 0, and X₁ is (CH₂-CH₂-O)₂.

3. The compounds or their compositions according to claim 1 or 2, for use as a medicament.

4. Use of the compounds or their compositions having general formula:

A-X_x-N0₂

for the treatment of septic shock, wherein A has the meanings reported in claim 1 or 2 and X₁ is a bivalent connecting bridge chosen from the following:

-YO- where Y is:

- aa linear or when permissible branched C₁C₂₀ alkylene, preferably having from 2 to 5 carbon atoms, - a cycloalkylene having from 5 to 7 carbon atoms optionally substituted;

-

- wherein n₂ is 0 or an integer from 1 to 3

-

- wherein nf' is an integer from 1 to 6, preferably from 2 to 4;

-

wherein R_1f = H, -CH₃ and nf is an integer from 1 to

6, preferably from 2 to 4.

Use of the compounds or their compositions having general formula: as antiinflammatory, wherein A has the meanings reported in claim 1 or 2 and X₁ is a bivalent connecting bridge chosen from the following:

-YO- where Y is:

- a linear or when permissible branched C₁-C₂₀ alkylene, preferably having from 2 to 5 carbon atoms, excluding this connecting bridge when R is:

. a radical of group II) except II_b) ;

. a radical of group III) except class of compounds of IIID)

. a radical of group IV);

. a a radical of group V), execpt X) and including -(CH₂)₄- for the compounds of formulae (III) and (IV);

- a cycloalkylene having from 5 to 7 carbon atoms optionally substituted; - wherein n₃ is 0 or an integer from 1 to 3 -

-

wherein nf is an integer from 1 to 6, preferably from 2 to 4

- wherein R_1f = H, -C_H3 and nf is an integer from 1 to 6, preferably from 2 to 4.

6. Use of the compounds or their compositions having general formula

A-X_x-NO₂

as anti-thrombotic wherein A has the meaning reported

in claim 1 or 2 and X₁ is a bivalent connecting bridge

chosen from the following:

-YO- where Y is:

- a linear or when permissible branched C₁-C₂₀ alkylene , preferably having from 2 to 5 carbon atoms, excluding this connecting bridge when R is:

. a radical of group II) except II_b);

. a radical of group III) except class of compounds of IIID)

. a radical of group IV);

. a radical of group V), except X) and including -(CH₂)₄- for the compounds of formulae (III) and (IV);

- a cycloalkylene having from 5 to 7 carbon atoms optionally substituted;

-

- wherein n₃ is 0 or an integer from 1 to 3

-

-

wherein nf is an integer from 1 to 6, preferably from 2 to 4

- wherein R_1f = H, -CH₃ and nf is an integer from 1 to

6, preferably from 2 to 4.

7. Use of the compounds or their compositions of the general formula:

A-X₁-NO₂

as analgesic wherein A has the meanings reported in claim l or 2 and X₁ is a bivalent connecting bridge chosen from the following:

-YO- where Y is:

- a linear or when permissible branched C₁-C₂₀ alkylene, preferably having from 2 to 5 carbon atoms, excluding this connecting bridge when R is:

. a radical of group II) except II_b) _;

. a radical of group III) except class of compounds of IIID) . a radical of group IV); . a radical of group V), except X) and including -(CH₂)₄- for the compounds of formulae (III) and (IV);

- a cycloalkylene having from 5 to 7 carbon atoms optionally substituted; wherein n₃ is 0 or an integer from 1 to 3

- -

wherein nf' is an integer from 1 to 6, preferably from 2 to 4

- wherein R_1f = H, -CH₃ and nf is an integer from 1 to 6, preferably from 2 to 4.