AU2021221158A1 - Methods for characterizing cells using gene expression and chromatin accessibility - Google Patents

Methods for characterizing cells using gene expression and chromatin accessibility Download PDF

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Publication number
AU2021221158A1
AU2021221158A1 AU2021221158A AU2021221158A AU2021221158A1 AU 2021221158 A1 AU2021221158 A1 AU 2021221158A1 AU 2021221158 A AU2021221158 A AU 2021221158A AU 2021221158 A AU2021221158 A AU 2021221158A AU 2021221158 A1 AU2021221158 A1 AU 2021221158A1
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Prior art keywords
cells
cell
nucleic acid
sequence
molecules
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AU2021221158A
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English (en)
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Arundhati Shamoni MAHESHWARI
Li Wang
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10X Genomics Inc
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10X Genomics Inc
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Publication of AU2021221158A1 publication Critical patent/AU2021221158A1/en
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    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
    • G16B20/30—Detection of binding sites or motifs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Theoretical Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Medical Informatics (AREA)
  • Evolutionary Biology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AU2021221158A 2020-02-13 2021-02-12 Methods for characterizing cells using gene expression and chromatin accessibility Pending AU2021221158A1 (en)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US202062976270P 2020-02-13 2020-02-13
US62/976,270 2020-02-13
US202062979986P 2020-02-21 2020-02-21
US62/979,986 2020-02-21
US202063114378P 2020-11-16 2020-11-16
US63/114,378 2020-11-16
US202063125331P 2020-12-14 2020-12-14
US63/125,331 2020-12-14
PCT/US2021/018020 WO2021163611A1 (en) 2020-02-13 2021-02-12 Methods for characterizing cells using gene expression and chromatin accessibility

Publications (1)

Publication Number Publication Date
AU2021221158A1 true AU2021221158A1 (en) 2022-09-22

Family

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AU2021221158A Pending AU2021221158A1 (en) 2020-02-13 2021-02-12 Methods for characterizing cells using gene expression and chromatin accessibility

Country Status (4)

Country Link
EP (1) EP4104177A1 (zh)
CN (1) CN115398547A (zh)
AU (1) AU2021221158A1 (zh)
WO (1) WO2021163611A1 (zh)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3788171B1 (en) 2018-05-03 2023-04-05 Becton, Dickinson and Company High throughput multiomics sample analysis

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AU7516694A (en) 1993-07-30 1995-02-28 Affymax Technologies N.V. Biotinylation of proteins
WO2008021123A1 (en) 2006-08-07 2008-02-21 President And Fellows Of Harvard College Fluorocarbon emulsion stabilizing surfactants
CA2881685C (en) 2012-08-14 2023-12-05 10X Genomics, Inc. Microcapsule compositions and methods
US20140378345A1 (en) 2012-08-14 2014-12-25 10X Technologies, Inc. Compositions and methods for sample processing
CN111394426B (zh) 2013-05-23 2024-05-10 斯坦福大学托管董事会 用于个人表观基因组学的至天然染色质的转座
BR112016023625A2 (pt) 2014-04-10 2018-06-26 10X Genomics, Inc. dispositivos fluídicos, sistemas e métodos para encapsular e particionar reagentes, e aplicações dos mesmos
JP6838969B2 (ja) 2014-06-26 2021-03-03 １０エックス ジェノミクス， インコーポレイテッド 個々の細胞または細胞集団由来の核酸の分析方法
SG10202012440VA (en) 2016-10-19 2021-01-28 10X Genomics Inc Methods and systems for barcoding nucleic acid molecules from individual cells or cell populations
US20190177800A1 (en) 2017-12-08 2019-06-13 10X Genomics, Inc. Methods and compositions for labeling cells
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11821027B2 (en) * 2017-01-10 2023-11-21 Juno Therapeutics, Inc. Epigenetic analysis of cell therapy and related methods
WO2018140966A1 (en) 2017-01-30 2018-08-02 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
US20180312822A1 (en) 2017-04-26 2018-11-01 10X Genomics, Inc. Mmlv reverse transcriptase variants
WO2018218226A1 (en) 2017-05-26 2018-11-29 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
US10844372B2 (en) 2017-05-26 2020-11-24 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
US10549279B2 (en) 2017-08-22 2020-02-04 10X Genomics, Inc. Devices having a plurality of droplet formation regions
US10590244B2 (en) 2017-10-04 2020-03-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
WO2019099751A1 (en) 2017-11-15 2019-05-23 10X Genomics, Inc. Functionalized gel beads
EP3749740B1 (en) * 2018-02-05 2023-08-30 The Board Of Trustees Of The Leland Stanford Junior University Systems and methods for multiplexed measurements in single and ensemble cells
CN112005115A (zh) 2018-02-12 2020-11-27 10X基因组学有限公司 表征来自单个细胞或细胞群体的多种分析物的方法
CN112262218A (zh) 2018-04-06 2021-01-22 10X基因组学有限公司 用于单细胞处理中的质量控制的系统和方法
WO2019204560A1 (en) * 2018-04-18 2019-10-24 The Regents Of The University Of California Method to connect chromatin accessibility and transcriptome

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Publication number Publication date
EP4104177A1 (en) 2022-12-21
WO2021163611A1 (en) 2021-08-19
CN115398547A (zh) 2022-11-25

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